cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_4XR-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2035.717 CPU sec, 80.279 real sec [M::process] read 684588 sequences (100000039 bp) in 1.084 CPU sec, 1.086 real sec... [M::process] read 684316 sequences (100000180 bp) in 1.190 CPU sec, 1.194 real sec... [M::process] read 684224 sequences (100000153 bp) in 1.147 CPU sec, 1.151 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 281668, 7, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 256, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (259.28, 123.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 294, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849) [M::mem_pestat] mean and std.dev: (314.92, 151.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1067) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 307, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848) [M::mem_pestat] mean and std.dev: (303.00, 130.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684276 sequences (100000115 bp) in 1.119 CPU sec, 1.116 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 281234, 16, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 214, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (242.62, 122.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 297, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (316.27, 150.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1069) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (985, 1057, 1685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3085) [M::mem_pestat] mean and std.dev: (1181.06, 571.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3785) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 404, 1007) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2543) [M::mem_pestat] mean and std.dev: (618.51, 528.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3311) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 281001, 10, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 261, 574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1386) [M::mem_pestat] mean and std.dev: (338.88, 269.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1792) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 297, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (315.92, 149.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1069) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 742, 1235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3623) [M::mem_pestat] mean and std.dev: (820.90, 748.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4817) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 272, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1125) [M::mem_pestat] mean and std.dev: (312.44, 180.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1436) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684588 reads in 232.855 CPU sec, 7.412 real sec [M::mem_process_seqs] Processed 684316 reads in 169.683 CPU sec, 5.454 real sec [M::mem_process_seqs] Processed 684224 reads in 185.744 CPU sec, 5.974 real sec sorting block 0, #reads = 1077743 mem = 536885225 peak = 597434368 [M::process] read 683730 sequences (100000274 bp) in 1.767 CPU sec, 2.019 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 281604, 9, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 253, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (232.31, 64.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 298, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (317.45, 150.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1077) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 282, 576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1318) [M::mem_pestat] mean and std.dev: (392.97, 311.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1689) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683584 sequences (100000077 bp) in 1.834 CPU sec, 1.907 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 281246, 11, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 164, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (198.03, 109.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 302, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (320.82, 152.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1091) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (421, 518, 684) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1210) [M::mem_pestat] mean and std.dev: (556.27, 261.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1604) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 254, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979) [M::mem_pestat] mean and std.dev: (267.06, 134.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1247) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683708 sequences (100000191 bp) in 1.369 CPU sec, 1.366 real sec... [M::mem_process_seqs] Processed 684276 reads in 163.353 CPU sec, 5.214 real sec sorting block 1, #reads = 1077414 mem = 536877121 peak = 809771008 [M::mem_process_seqs] Processed 683730 reads in 105.924 CPU sec, 3.382 real sec sorting block 2, #reads = 1077908 mem = 536880419 peak = 867328000 [M::process] read 683908 sequences (100000190 bp) in 1.803 CPU sec, 1.880 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 281604, 7, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 265, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1233) [M::mem_pestat] mean and std.dev: (349.80, 249.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 304, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (322.85, 154.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1100) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 211, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (229.77, 97.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682334 sequences (100000281 bp) in 1.720 CPU sec, 1.737 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282411, 10, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (325, 526, 1037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2461) [M::mem_pestat] mean and std.dev: (464.32, 280.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3173) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 302, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (321.35, 153.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (574, 2763, 3071) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8065) [M::mem_pestat] mean and std.dev: (2733.10, 2188.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11488) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 316, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1049) [M::mem_pestat] mean and std.dev: (282.05, 129.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1335) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 281820, 10, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 224, 902) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2392) [M::mem_pestat] mean and std.dev: (444.05, 403.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3137) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 303, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (323.22, 155.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (487, 487, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (487, 487) [M::mem_pestat] mean and std.dev: (487.00, 0.00) [M::mem_pestat] low and high boundaries for proper pairs: (487, 487) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 349, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1089) [M::mem_pestat] mean and std.dev: (338.30, 189.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1396) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683584 reads in 230.837 CPU sec, 7.387 real sec [M::mem_process_seqs] Processed 683908 reads in 173.673 CPU sec, 5.588 real sec [M::mem_process_seqs] Processed 683708 reads in 189.669 CPU sec, 6.123 real sec sorting block 3, #reads = 1077928 mem = 536878500 peak = 867328000 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 281162, 5, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 339, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1208) [M::mem_pestat] mean and std.dev: (327.69, 177.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 312, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (330.09, 156.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 235, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (236.80, 99.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681778 sequences (100000267 bp) in 2.550 CPU sec, 2.750 real sec... [M::mem_process_seqs] Processed 682334 reads in 87.206 CPU sec, 2.910 real sec sorting block 4, #reads = 1077661 mem = 536879096 peak = 867328000 [M::process] read 681600 sequences (100000076 bp) in 1.926 CPU sec, 2.055 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 281039, 11, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 271, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1265) [M::mem_pestat] mean and std.dev: (319.08, 206.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 315, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (332.58, 156.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (561, 908, 1909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4605) [M::mem_pestat] mean and std.dev: (1074.09, 799.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5953) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 318, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (287.67, 106.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681304 sequences (100000154 bp) in 1.705 CPU sec, 1.739 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 281056, 0, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 235, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (205.92, 67.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 318, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (334.93, 157.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 279, 607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1405) [M::mem_pestat] mean and std.dev: (400.17, 301.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1804) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681778 reads in 157.197 CPU sec, 4.979 real sec [M::process] read 681530 sequences (100000030 bp) in 1.772 CPU sec, 1.792 real sec... sorting block 5, #reads = 1077412 mem = 536880584 peak = 867328000 [M::mem_process_seqs] Processed 681600 reads in 102.900 CPU sec, 3.246 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 280216, 8, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 298, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (253.00, 106.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 317, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (334.51, 156.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 309, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (277.14, 103.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 6, #reads = 1076137 mem = 536885951 peak = 867328000 [M::process] read 681290 sequences (100000212 bp) in 2.404 CPU sec, 2.545 real sec... [M::process] read 682094 sequences (100000208 bp) in 1.369 CPU sec, 1.369 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 280920, 3, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 288, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (312.32, 175.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 317, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (333.22, 155.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 365, 633) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1549) [M::mem_pestat] mean and std.dev: (366.36, 202.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2007) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681304 reads in 160.227 CPU sec, 5.114 real sec [M::mem_process_seqs] Processed 681530 reads in 101.353 CPU sec, 3.234 real sec sorting block 7, #reads = 1077038 mem = 536880181 peak = 867328000 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 280934, 13, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 190, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (221.65, 114.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 318, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (334.38, 156.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (580, 855, 1602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3646) [M::mem_pestat] mean and std.dev: (978.38, 484.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4668) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 253, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (255.92, 95.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680634 sequences (100000017 bp) in 2.414 CPU sec, 2.557 real sec... [M::process] read 680674 sequences (100000148 bp) in 1.359 CPU sec, 1.361 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 280692, 4, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 280, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (284.49, 128.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 308, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (327.31, 154.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 220, 688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1716) [M::mem_pestat] mean and std.dev: (258.34, 183.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2230) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681290 reads in 165.361 CPU sec, 5.283 real sec [M::mem_process_seqs] Processed 682094 reads in 105.221 CPU sec, 3.359 real sec sorting block 8, #reads = 1076990 mem = 536882736 peak = 867328000 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 279999, 9, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 408, 1423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3779) [M::mem_pestat] mean and std.dev: (808.18, 663.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4957) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 318, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (333.89, 154.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 337, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (319.45, 132.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680534 sequences (100000024 bp) in 2.090 CPU sec, 2.217 real sec... [M::process] read 680106 sequences (100000219 bp) in 1.410 CPU sec, 1.409 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 280513, 8, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 264, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (240.08, 104.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 319, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (335.86, 156.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 212, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1150) [M::mem_pestat] mean and std.dev: (327.39, 227.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1475) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680634 reads in 165.486 CPU sec, 5.308 real sec [M::mem_process_seqs] Processed 680674 reads in 104.641 CPU sec, 3.360 real sec sorting block 9, #reads = 1076260 mem = 536880142 peak = 867328000 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 280143, 11, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (196.97, 78.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 320, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (336.55, 156.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (384, 676, 1206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2850) [M::mem_pestat] mean and std.dev: (753.27, 439.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3672) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 306, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016) [M::mem_pestat] mean and std.dev: (329.56, 196.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1278) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680244 sequences (100000236 bp) in 2.272 CPU sec, 2.416 real sec... [M::process] read 680254 sequences (100000292 bp) in 1.458 CPU sec, 1.459 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 280215, 11, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 242, 809) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2061) [M::mem_pestat] mean and std.dev: (436.07, 438.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 321, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (337.01, 155.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1056, 1239, 1766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3186) [M::mem_pestat] mean and std.dev: (1089.10, 599.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3896) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 246, 589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1409) [M::mem_pestat] mean and std.dev: (301.57, 181.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1819) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680534 reads in 166.321 CPU sec, 5.323 real sec sorting block 10, #reads = 1075562 mem = 536882033 peak = 867328000 [M::mem_process_seqs] Processed 680106 reads in 106.970 CPU sec, 3.419 real sec sorting block 11, #reads = 1075986 mem = 536879974 peak = 867328000 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 279980, 5, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 346, 1002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2678) [M::mem_pestat] mean and std.dev: (449.06, 447.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 320, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (335.38, 154.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (143, 257, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (222.83, 88.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680746 sequences (100000160 bp) in 2.465 CPU sec, 2.614 real sec... [M::process] read 680506 sequences (100000188 bp) in 1.560 CPU sec, 1.558 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 280763, 8, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 357, 1628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4448) [M::mem_pestat] mean and std.dev: (645.13, 720.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5858) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 318, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (334.36, 154.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 263, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1129) [M::mem_pestat] mean and std.dev: (283.58, 152.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1437) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680244 reads in 169.289 CPU sec, 5.413 real sec [M::mem_process_seqs] Processed 680254 reads in 109.733 CPU sec, 3.500 real sec sorting block 12, #reads = 1075537 mem = 536880033 peak = 867328000 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 280551, 20, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 288, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056) [M::mem_pestat] mean and std.dev: (299.86, 167.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1350) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 318, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (333.97, 154.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (461, 871, 2434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6380) [M::mem_pestat] mean and std.dev: (1265.55, 887.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8353) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (253, 501, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018) [M::mem_pestat] mean and std.dev: (382.13, 138.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1273) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680444 sequences (100000191 bp) in 2.484 CPU sec, 2.652 real sec... [M::process] read 680280 sequences (100000237 bp) in 1.880 CPU sec, 1.878 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 280550, 5, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 287, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (304.45, 190.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1167) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 317, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (333.19, 153.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1104) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 224, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (251.62, 119.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680746 reads in 170.394 CPU sec, 5.440 real sec [M::mem_process_seqs] Processed 680506 reads in 110.808 CPU sec, 3.529 real sec sorting block 13, #reads = 1076280 mem = 536878834 peak = 867328000 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 280124, 13, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 282, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (235.34, 96.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 315, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (331.59, 153.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (84, 470, 1090) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3102) [M::mem_pestat] mean and std.dev: (696.77, 716.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4108) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 203, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1284) [M::mem_pestat] mean and std.dev: (299.52, 217.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1663) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 679928 sequences (100000248 bp) in 2.543 CPU sec, 2.706 real sec... [M::process] read 679872 sequences (100000060 bp) in 1.484 CPU sec, 1.491 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 280179, 11, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 231, 702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1764) [M::mem_pestat] mean and std.dev: (273.78, 184.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2295) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 318, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (333.41, 153.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1097) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 619, 656) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1940) [M::mem_pestat] mean and std.dev: (494.55, 513.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2582) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (136, 293, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1216) [M::mem_pestat] mean and std.dev: (328.40, 260.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1576) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680444 reads in 172.449 CPU sec, 5.519 real sec [M::mem_process_seqs] Processed 680280 reads in 105.217 CPU sec, 3.363 real sec sorting block 14, #reads = 1075723 mem = 536879260 peak = 867328000 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 280099, 6, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 191, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1140) [M::mem_pestat] mean and std.dev: (249.52, 145.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 321, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (336.41, 155.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 382, 599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1453) [M::mem_pestat] mean and std.dev: (404.18, 264.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1880) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679572 sequences (100000154 bp) in 2.820 CPU sec, 2.981 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 280122, 9, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (306, 340, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (357.79, 161.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1138) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 319, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (335.51, 154.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 268, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (312.76, 144.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679666 sequences (100000086 bp) in 1.609 CPU sec, 1.604 real sec... [M::mem_process_seqs] Processed 679928 reads in 158.244 CPU sec, 5.035 real sec sorting block 15, #reads = 1076171 mem = 536876620 peak = 867328000 [M::mem_process_seqs] Processed 679872 reads in 103.352 CPU sec, 3.271 real sec sorting block 16, #reads = 1074978 mem = 536877863 peak = 948678656 [M::process] read 684126 sequences (100000094 bp) in 2.731 CPU sec, 2.905 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 280979, 3, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 271, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (271.40, 146.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 324, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (339.42, 156.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 341, 699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1767) [M::mem_pestat] mean and std.dev: (331.10, 218.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2301) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684140 sequences (100000254 bp) in 1.760 CPU sec, 1.751 real sec... [M::mem_process_seqs] Processed 679666 reads in 97.290 CPU sec, 3.075 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 280218, 10, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 339, 1881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5319) [M::mem_pestat] mean and std.dev: (987.62, 1330.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7038) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 322, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (337.20, 155.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1104) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (342, 1469, 1469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3723) [M::mem_pestat] mean and std.dev: (1200.20, 778.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4850) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 270, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (259.73, 97.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 281760, 11, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 275, 991) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2645) [M::mem_pestat] mean and std.dev: (447.92, 458.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 300, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (319.82, 153.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1093) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1800, 1814, 2028) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1344, 2484) [M::mem_pestat] mean and std.dev: (1894.86, 90.69) [M::mem_pestat] low and high boundaries for proper pairs: (1116, 2712) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 262, 733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1861) [M::mem_pestat] mean and std.dev: (418.91, 383.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2425) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684018 sequences (100000180 bp) in 1.663 CPU sec, 1.668 real sec... [M::mem_process_seqs] Processed 679572 reads in 255.752 CPU sec, 8.128 real sec sorting block 17, #reads = 1076370 mem = 536880868 peak = 948678656 [M::mem_process_seqs] Processed 684126 reads in 127.087 CPU sec, 4.054 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282013, 17, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 251, 999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2593) [M::mem_pestat] mean and std.dev: (413.11, 378.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3390) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 298, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (318.23, 152.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1081) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (526, 2133, 2585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6703) [M::mem_pestat] mean and std.dev: (1710.18, 1213.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8762) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 319, 754) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1784) [M::mem_pestat] mean and std.dev: (452.67, 329.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2299) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 18, #reads = 1077069 mem = 536881114 peak = 948678656 [M::process] read 683798 sequences (100000197 bp) in 2.747 CPU sec, 2.918 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 281897, 2, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 359, 685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1657) [M::mem_pestat] mean and std.dev: (491.10, 429.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2208) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 302, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (321.07, 153.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 193, 704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1820) [M::mem_pestat] mean and std.dev: (381.97, 372.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2378) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684140 reads in 159.932 CPU sec, 5.075 real sec [M::process] read 683602 sequences (100000058 bp) in 1.840 CPU sec, 1.844 real sec... [M::mem_process_seqs] Processed 684018 reads in 103.822 CPU sec, 3.288 real sec sorting block 19, #reads = 1079394 mem = 536879384 peak = 948678656 [M::process] read 683184 sequences (100000041 bp) in 2.446 CPU sec, 2.629 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 281748, 3, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 217, 1919) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5485) [M::mem_pestat] mean and std.dev: (702.10, 825.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7268) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 304, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (322.52, 153.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1100) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 377, 824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2070) [M::mem_pestat] mean and std.dev: (540.97, 475.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2693) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683032 sequences (100000106 bp) in 1.880 CPU sec, 1.881 real sec... [M::mem_process_seqs] Processed 683602 reads in 91.340 CPU sec, 2.882 real sec sorting block 20, #reads = 1078403 mem = 536879839 peak = 948678656 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281464, 7, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 314, 921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2377) [M::mem_pestat] mean and std.dev: (436.32, 420.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3105) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 302, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (321.31, 153.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 263, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1230) [M::mem_pestat] mean and std.dev: (329.12, 245.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1584) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 281023, 4, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 220, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (241.10, 125.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 307, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (325.34, 154.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 300, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (276.83, 142.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683798 reads in 250.124 CPU sec, 7.945 real sec [M::process] read 682870 sequences (100000104 bp) in 2.157 CPU sec, 2.193 real sec... [M::mem_process_seqs] Processed 683184 reads in 122.008 CPU sec, 3.882 real sec sorting block 21, #reads = 1078410 mem = 536879196 peak = 948678656 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 281134, 14, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 189, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (236.90, 119.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 309, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (326.56, 154.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (852, 1136, 1456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2664) [M::mem_pestat] mean and std.dev: (1076.14, 446.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3268) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 198, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (248.08, 135.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682430 sequences (100000301 bp) in 2.933 CPU sec, 3.144 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 281482, 12, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 279, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (281.47, 120.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1026) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 311, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (329.95, 157.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (583, 1650, 1650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3784) [M::mem_pestat] mean and std.dev: (1380.33, 655.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4851) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 337, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1092) [M::mem_pestat] mean and std.dev: (367.12, 260.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1408) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683032 reads in 163.350 CPU sec, 5.197 real sec [M::process] read 681932 sequences (100000017 bp) in 1.599 CPU sec, 1.601 real sec... [M::mem_process_seqs] Processed 682870 reads in 105.748 CPU sec, 3.343 real sec sorting block 22, #reads = 1078631 mem = 536880657 peak = 948678656 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281670, 6, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (271, 321, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943) [M::mem_pestat] mean and std.dev: (380.40, 175.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1167) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 315, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (332.93, 158.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1142) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 372, 833) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2097) [M::mem_pestat] mean and std.dev: (547.07, 474.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2729) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681462 sequences (100000039 bp) in 2.772 CPU sec, 3.020 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 281037, 10, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 236, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (277.41, 204.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 318, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (335.08, 158.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (444, 2655, 6341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18135) [M::mem_pestat] mean and std.dev: (2858.90, 2432.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 24032) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 261, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075) [M::mem_pestat] mean and std.dev: (308.09, 181.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1369) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681416 sequences (100000206 bp) in 1.820 CPU sec, 1.812 real sec... [M::mem_process_seqs] Processed 682430 reads in 172.630 CPU sec, 5.513 real sec [M::mem_process_seqs] Processed 681932 reads in 114.497 CPU sec, 3.654 real sec sorting block 23, #reads = 1077749 mem = 536877630 peak = 948678656 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 281100, 7, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 175, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (71, 286) [M::mem_pestat] mean and std.dev: (163.04, 33.06) [M::mem_pestat] low and high boundaries for proper pairs: (28, 329) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 321, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (337.56, 159.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1150) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 214, 578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1380) [M::mem_pestat] mean and std.dev: (331.98, 229.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1781) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681414 sequences (100000296 bp) in 2.611 CPU sec, 2.906 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 280172, 8, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 267, 2041) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5651) [M::mem_pestat] mean and std.dev: (955.12, 1114.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 321, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (337.13, 158.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 194, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (233.30, 105.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680816 sequences (100000278 bp) in 1.454 CPU sec, 1.475 real sec... [M::mem_process_seqs] Processed 681462 reads in 178.029 CPU sec, 5.707 real sec sorting block 24, #reads = 1078095 mem = 536879083 peak = 948678656 [M::mem_process_seqs] Processed 681416 reads in 113.439 CPU sec, 3.630 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 280780, 6, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (253, 373, 1690) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4564) [M::mem_pestat] mean and std.dev: (1048.23, 1378.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 321, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (336.73, 157.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1142) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (139, 170, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937) [M::mem_pestat] mean and std.dev: (273.26, 178.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1203) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 25, #reads = 1076596 mem = 536879433 peak = 948678656 [M::process] read 681142 sequences (100000273 bp) in 2.724 CPU sec, 3.006 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 281180, 9, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 298, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1172) [M::mem_pestat] mean and std.dev: (356.72, 277.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 322, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (337.82, 157.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1136) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 239, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (43, 433) [M::mem_pestat] mean and std.dev: (228.42, 70.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680536 sequences (100000201 bp) in 1.662 CPU sec, 1.675 real sec... [M::mem_process_seqs] Processed 681414 reads in 171.775 CPU sec, 5.495 real sec [M::mem_process_seqs] Processed 680816 reads in 114.488 CPU sec, 3.657 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 280882, 3, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 290, 887) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2287) [M::mem_pestat] mean and std.dev: (445.50, 402.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2987) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 319, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (335.12, 155.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 352, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (349.47, 176.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1062) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 26, #reads = 1076467 mem = 536877221 peak = 948678656 [M::process] read 680362 sequences (100000059 bp) in 3.146 CPU sec, 3.440 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 280408, 11, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 224, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (216.65, 89.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 322, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (338.03, 156.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (3, 318, 555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1659) [M::mem_pestat] mean and std.dev: (412.18, 545.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2594) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 295, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (264.33, 73.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681142 reads in 174.287 CPU sec, 5.587 real sec [M::mem_process_seqs] Processed 680536 reads in 116.484 CPU sec, 3.695 real sec [M::process] read 680370 sequences (100000162 bp) in 2.044 CPU sec, 2.154 real sec... sorting block 27, #reads = 1076039 mem = 536880224 peak = 948678656 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 280607, 17, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 378, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021) [M::mem_pestat] mean and std.dev: (361.67, 178.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1286) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 323, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (338.04, 155.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1123) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (784, 1268, 2204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5044) [M::mem_pestat] mean and std.dev: (1219.13, 718.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6464) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 268, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (334.93, 197.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680336 sequences (100000023 bp) in 2.898 CPU sec, 3.167 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 280426, 8, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 342, 650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1578) [M::mem_pestat] mean and std.dev: (421.28, 310.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2042) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 321, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (336.54, 155.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 325, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (285.32, 116.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680362 reads in 172.588 CPU sec, 5.542 real sec [M::mem_process_seqs] Processed 680370 reads in 115.765 CPU sec, 3.680 real sec [M::process] read 680106 sequences (100000167 bp) in 2.240 CPU sec, 2.291 real sec... sorting block 28, #reads = 1075920 mem = 536878845 peak = 948678656 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 280211, 6, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 279, 951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2463) [M::mem_pestat] mean and std.dev: (600.92, 727.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 322, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (337.80, 156.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 269, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (286.16, 148.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680304 sequences (100000207 bp) in 2.901 CPU sec, 3.074 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 280996, 16, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 251, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (254.26, 112.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 322, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (337.04, 154.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (635, 1424, 1754) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3992) [M::mem_pestat] mean and std.dev: (1067.93, 749.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5111) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 320, 575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1301) [M::mem_pestat] mean and std.dev: (387.31, 271.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1664) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680336 reads in 170.301 CPU sec, 5.471 real sec sorting block 29, #reads = 1075076 mem = 536879148 peak = 948678656 [M::mem_process_seqs] Processed 680106 reads in 112.616 CPU sec, 3.576 real sec [M::process] read 680434 sequences (100000149 bp) in 1.666 CPU sec, 1.732 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 280182, 18, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 355, 1208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3164) [M::mem_pestat] mean and std.dev: (648.92, 551.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4142) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 320, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (335.36, 154.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (170, 910, 2535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7265) [M::mem_pestat] mean and std.dev: (1389.67, 1197.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9630) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 293, 827) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2159) [M::mem_pestat] mean and std.dev: (483.39, 418.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2825) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 30, #reads = 1076345 mem = 536884347 peak = 948678656 [M::process] read 680414 sequences (100000191 bp) in 2.787 CPU sec, 2.947 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 280657, 8, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 256, 548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1346) [M::mem_pestat] mean and std.dev: (363.55, 295.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1745) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 319, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (333.70, 153.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1104) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (116, 196, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (198.50, 91.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680304 reads in 152.835 CPU sec, 4.860 real sec [M::mem_process_seqs] Processed 680434 reads in 99.056 CPU sec, 3.121 real sec [M::process] read 680598 sequences (100000209 bp) in 1.992 CPU sec, 2.061 real sec... sorting block 31, #reads = 1074965 mem = 536878193 peak = 948678656 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 280225, 5, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 213, 803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2107) [M::mem_pestat] mean and std.dev: (367.94, 277.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2759) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 318, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (333.43, 153.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1104) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 300, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (292.21, 156.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1087) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680522 sequences (100000096 bp) in 2.505 CPU sec, 2.644 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 280312, 4, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 304, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (314.41, 184.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1165) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 317, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (332.79, 153.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 252, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (247.38, 114.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 995) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680414 reads in 153.111 CPU sec, 4.872 real sec [M::process] read 680532 sequences (100000075 bp) in 1.866 CPU sec, 1.887 real sec... [M::mem_process_seqs] Processed 680598 reads in 101.086 CPU sec, 3.203 real sec sorting block 32, #reads = 1075069 mem = 536878129 peak = 948678656 [M::process] read 680202 sequences (100000133 bp) in 2.805 CPU sec, 2.990 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 280966, 6, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 283, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (233.46, 95.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 317, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (333.24, 153.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1104) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 302, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066) [M::mem_pestat] mean and std.dev: (310.26, 204.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1370) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680532 reads in 95.496 CPU sec, 3.005 real sec [M::process] read 680540 sequences (100000130 bp) in 1.889 CPU sec, 1.893 real sec... sorting block 33, #reads = 1075053 mem = 536879336 peak = 948678656 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 280850, 15, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 240, 602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1444) [M::mem_pestat] mean and std.dev: (423.90, 427.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2135) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 316, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (332.33, 153.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (239, 286, 1284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3374) [M::mem_pestat] mean and std.dev: (715.07, 836.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4419) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 310, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1211) [M::mem_pestat] mean and std.dev: (326.47, 174.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1554) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 280556, 7, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 261, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (285.59, 150.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 319, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (334.93, 154.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 269, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1132) [M::mem_pestat] mean and std.dev: (314.25, 181.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1440) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680522 reads in 263.842 CPU sec, 8.393 real sec [M::process] read 680134 sequences (100000075 bp) in 2.077 CPU sec, 2.109 real sec... [M::mem_process_seqs] Processed 680202 reads in 135.769 CPU sec, 4.340 real sec sorting block 34, #reads = 1075350 mem = 536879773 peak = 948678656 [M::process] read 683250 sequences (100000152 bp) in 3.210 CPU sec, 3.476 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 280445, 7, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 204, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (239.34, 116.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 322, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (338.30, 157.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 354, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1175) [M::mem_pestat] mean and std.dev: (346.16, 258.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1503) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680134 reads in 99.263 CPU sec, 3.139 real sec [M::process] read 685084 sequences (100000134 bp) in 2.144 CPU sec, 2.227 real sec... sorting block 35, #reads = 1074770 mem = 536880052 peak = 948678656 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 280645, 13, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 267, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (261.00, 165.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1007) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 317, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (333.90, 155.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (602, 1461, 3005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7811) [M::mem_pestat] mean and std.dev: (1800.23, 1334.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10214) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 268, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (304.68, 164.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281408, 8, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 174, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039) [M::mem_pestat] mean and std.dev: (231.59, 180.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1338) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 305, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (323.91, 154.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 239, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (252.81, 102.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680540 reads in 270.833 CPU sec, 8.672 real sec [M::mem_process_seqs] Processed 683250 reads in 135.336 CPU sec, 4.313 real sec [M::process] read 684428 sequences (100000126 bp) in 2.394 CPU sec, 2.535 real sec... sorting block 36, #reads = 1073369 mem = 536878248 peak = 948678656 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 282345, 3, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 241, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (234.63, 75.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 297, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (317.29, 152.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1087) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 315, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (314.64, 162.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1189) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683846 sequences (100000268 bp) in 2.695 CPU sec, 2.879 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 281642, 2, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 284, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (235.68, 76.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 299, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (319.39, 153.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1093) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 287, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1195) [M::mem_pestat] mean and std.dev: (350.72, 219.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1521) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683650 sequences (100000234 bp) in 1.626 CPU sec, 1.633 real sec... [M::mem_process_seqs] Processed 685084 reads in 177.494 CPU sec, 5.651 real sec [M::mem_process_seqs] Processed 684428 reads in 117.057 CPU sec, 3.720 real sec sorting block 37, #reads = 1078496 mem = 536878624 peak = 948678656 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 281904, 7, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 364, 984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2588) [M::mem_pestat] mean and std.dev: (491.88, 505.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3390) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 302, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (320.92, 152.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1091) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 314, 767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1875) [M::mem_pestat] mean and std.dev: (359.77, 289.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2429) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683434 sequences (100000026 bp) in 2.930 CPU sec, 3.128 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 281139, 3, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 315, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994) [M::mem_pestat] mean and std.dev: (328.21, 211.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1271) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 303, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (321.24, 152.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1092) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 298, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (296.85, 128.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683846 reads in 175.634 CPU sec, 5.605 real sec [M::process] read 683134 sequences (100000107 bp) in 1.762 CPU sec, 1.818 real sec... sorting block 38, #reads = 1077473 mem = 536886155 peak = 948678656 [M::mem_process_seqs] Processed 683650 reads in 115.991 CPU sec, 3.695 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 281729, 4, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (243, 390, 562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1200) [M::mem_pestat] mean and std.dev: (375.70, 215.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 305, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (322.62, 152.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1093) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 373, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (286.44, 105.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 990) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 39, #reads = 1076454 mem = 536878202 peak = 972566528 [M::process] read 682574 sequences (100000067 bp) in 2.432 CPU sec, 2.576 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 281453, 14, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 276, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1187) [M::mem_pestat] mean and std.dev: (299.22, 183.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 308, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (325.69, 153.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (8, 97, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1409) [M::mem_pestat] mean and std.dev: (188.00, 236.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1876) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 348, 676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1666) [M::mem_pestat] mean and std.dev: (398.26, 321.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2161) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682632 sequences (100000246 bp) in 1.796 CPU sec, 1.799 real sec... [M::mem_process_seqs] Processed 683434 reads in 171.545 CPU sec, 5.479 real sec [M::mem_process_seqs] Processed 683134 reads in 112.691 CPU sec, 3.586 real sec sorting block 40, #reads = 1077624 mem = 536879062 peak = 972566528 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 281194, 15, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 199, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (197.22, 81.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 312, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (329.22, 155.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (476, 476, 703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (22, 1157) [M::mem_pestat] mean and std.dev: (540.47, 279.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1659) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 247, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (299.00, 167.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1089) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682044 sequences (100000246 bp) in 2.810 CPU sec, 2.978 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 281386, 9, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 245, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (236.21, 98.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1189) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 313, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (331.56, 157.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 299, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1096) [M::mem_pestat] mean and std.dev: (316.03, 182.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1408) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681972 sequences (100000160 bp) in 1.870 CPU sec, 1.878 real sec... [M::mem_process_seqs] Processed 682574 reads in 169.549 CPU sec, 5.393 real sec [M::mem_process_seqs] Processed 682632 reads in 112.182 CPU sec, 3.601 real sec sorting block 41, #reads = 1076138 mem = 536877632 peak = 972566528 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 281360, 9, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 249, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1315) [M::mem_pestat] mean and std.dev: (329.52, 304.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 316, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (334.53, 158.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 208, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (44, 364) [M::mem_pestat] mean and std.dev: (196.71, 45.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 428) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681446 sequences (100000124 bp) in 2.779 CPU sec, 3.071 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 281506, 16, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 359, 656) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1564) [M::mem_pestat] mean and std.dev: (326.37, 168.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2018) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 319, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (336.38, 159.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (258, 1848, 2607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7305) [M::mem_pestat] mean and std.dev: (1592.56, 1642.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9654) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 250, 988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2576) [M::mem_pestat] mean and std.dev: (410.35, 469.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3370) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682044 reads in 174.517 CPU sec, 5.739 real sec [M::process] read 681726 sequences (100000053 bp) in 1.687 CPU sec, 1.832 real sec... [M::mem_process_seqs] Processed 681972 reads in 116.733 CPU sec, 4.073 real sec sorting block 42, #reads = 1076116 mem = 536878237 peak = 972566528 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 280990, 18, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 209, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033) [M::mem_pestat] mean and std.dev: (296.17, 241.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1330) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 319, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (336.79, 159.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 129, 1169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3455) [M::mem_pestat] mean and std.dev: (674.00, 990.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4635) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 286, 624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1406) [M::mem_pestat] mean and std.dev: (338.18, 232.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1797) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681670 sequences (100000135 bp) in 2.914 CPU sec, 3.175 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 281000, 10, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 262, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (305.29, 214.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1176) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 319, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (336.14, 159.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (277, 969, 1757) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4717) [M::mem_pestat] mean and std.dev: (1003.50, 809.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6197) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 242, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (237.80, 98.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681446 reads in 177.594 CPU sec, 5.913 real sec [M::process] read 681310 sequences (100000264 bp) in 1.687 CPU sec, 1.819 real sec... sorting block 43, #reads = 1076893 mem = 536879389 peak = 972566528 [M::mem_process_seqs] Processed 681726 reads in 119.010 CPU sec, 4.121 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 281361, 3, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 230, 548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1330) [M::mem_pestat] mean and std.dev: (253.64, 168.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 319, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (336.68, 159.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (244, 420, 639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1429) [M::mem_pestat] mean and std.dev: (361.43, 172.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1824) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 44, #reads = 1076361 mem = 536878198 peak = 1017032704 [M::process] read 681298 sequences (100000027 bp) in 2.875 CPU sec, 3.171 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 281072, 14, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 238, 643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1465) [M::mem_pestat] mean and std.dev: (353.86, 268.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1876) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 320, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (337.05, 158.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (122, 846, 2618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7610) [M::mem_pestat] mean and std.dev: (1580.86, 1523.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10106) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 286, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042) [M::mem_pestat] mean and std.dev: (359.05, 262.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1410) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681670 reads in 176.820 CPU sec, 5.914 real sec [M::mem_process_seqs] Processed 681310 reads in 117.521 CPU sec, 4.028 real sec [M::process] read 681190 sequences (100000235 bp) in 2.095 CPU sec, 2.144 real sec... sorting block 45, #reads = 1075749 mem = 536878334 peak = 1017032704 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 280486, 11, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 227, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971) [M::mem_pestat] mean and std.dev: (288.94, 204.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1241) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 318, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (335.66, 158.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (2402, 2402, 4471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8609) [M::mem_pestat] mean and std.dev: (2655.00, 1265.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10678) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (288, 308, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (180, 450) [M::mem_pestat] mean and std.dev: (291.38, 42.41) [M::mem_pestat] low and high boundaries for proper pairs: (122, 504) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681424 sequences (100000128 bp) in 2.498 CPU sec, 2.741 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 280673, 14, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (266, 383, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1127) [M::mem_pestat] mean and std.dev: (395.19, 229.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1414) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 317, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (334.51, 157.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (96, 471, 843) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2337) [M::mem_pestat] mean and std.dev: (532.00, 466.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3084) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 260, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (303.83, 162.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1155) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681298 reads in 179.499 CPU sec, 6.057 real sec [M::process] read 681502 sequences (100000164 bp) in 1.864 CPU sec, 1.985 real sec... [M::mem_process_seqs] Processed 681190 reads in 120.158 CPU sec, 4.245 real sec sorting block 46, #reads = 1076571 mem = 536882146 peak = 1017032704 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 280802, 15, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 272, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (290.14, 142.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1143) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 317, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (334.53, 157.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (2029, 2029, 4361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9025) [M::mem_pestat] mean and std.dev: (2332.53, 1547.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11357) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 227, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982) [M::mem_pestat] mean and std.dev: (271.64, 144.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1255) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681122 sequences (100000203 bp) in 2.485 CPU sec, 2.750 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 280577, 21, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 296, 1158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3068) [M::mem_pestat] mean and std.dev: (569.68, 514.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4023) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 315, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (333.07, 157.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (797, 1018, 3921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10169) [M::mem_pestat] mean and std.dev: (2341.95, 2111.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13293) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 323, 593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1317) [M::mem_pestat] mean and std.dev: (381.53, 248.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1679) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681424 reads in 178.091 CPU sec, 6.228 real sec [M::mem_process_seqs] Processed 681502 reads in 118.447 CPU sec, 4.155 real sec sorting block 47, #reads = 1076102 mem = 536880196 peak = 1017032704 [M::process] read 681412 sequences (100000038 bp) in 2.218 CPU sec, 2.449 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 280682, 8, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 379, 801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1985) [M::mem_pestat] mean and std.dev: (432.31, 310.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 312, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (330.43, 155.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 293, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1160) [M::mem_pestat] mean and std.dev: (275.00, 146.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1481) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681266 sequences (100000144 bp) in 2.572 CPU sec, 2.998 real sec... sorting block 48, #reads = 1075864 mem = 536878471 peak = 1017032704 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 280643, 8, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 245, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (271.08, 128.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 312, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (330.35, 155.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1104) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (129, 234, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (260.66, 160.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681122 reads in 178.708 CPU sec, 6.107 real sec [M::mem_process_seqs] Processed 681412 reads in 118.812 CPU sec, 4.164 real sec [M::process] read 681274 sequences (100000112 bp) in 1.966 CPU sec, 2.172 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 280281, 5, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 245, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (248.48, 102.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 314, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (332.47, 156.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 404, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (313.91, 113.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 49, #reads = 1075764 mem = 536878542 peak = 1017032704 [M::process] read 680982 sequences (100000271 bp) in 2.786 CPU sec, 3.080 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 280865, 2, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 237, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (268.76, 106.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1062) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 314, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (331.06, 154.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 273, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040) [M::mem_pestat] mean and std.dev: (324.31, 172.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1314) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681266 reads in 181.332 CPU sec, 6.128 real sec [M::mem_process_seqs] Processed 681274 reads in 120.824 CPU sec, 4.029 real sec [M::process] read 680814 sequences (100000066 bp) in 2.107 CPU sec, 2.309 real sec... sorting block 50, #reads = 1075616 mem = 536877234 peak = 1017032704 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 280301, 11, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (271, 421, 1857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5029) [M::mem_pestat] mean and std.dev: (1002.15, 1198.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 316, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (332.65, 154.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (777, 891, 2681) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6489) [M::mem_pestat] mean and std.dev: (1169.67, 854.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8393) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 249, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (260.30, 84.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680756 sequences (100000015 bp) in 2.519 CPU sec, 2.858 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 280709, 6, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 250, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1211) [M::mem_pestat] mean and std.dev: (264.41, 183.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 318, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (333.96, 155.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 323, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (314.81, 134.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680982 reads in 178.402 CPU sec, 5.923 real sec [M::mem_process_seqs] Processed 680814 reads in 116.456 CPU sec, 3.807 real sec [M::process] read 680634 sequences (100000035 bp) in 1.781 CPU sec, 1.848 real sec... sorting block 51, #reads = 1073816 mem = 536878735 peak = 1017032704 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 280707, 12, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 303, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (287.60, 139.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1159) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 319, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (335.73, 156.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (781, 2167, 2264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5230) [M::mem_pestat] mean and std.dev: (1417.45, 807.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6713) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (238, 308, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976) [M::mem_pestat] mean and std.dev: (317.67, 132.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1222) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680580 sequences (100000221 bp) in 2.459 CPU sec, 2.733 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 280525, 4, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 294, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992) [M::mem_pestat] mean and std.dev: (262.97, 160.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1275) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 319, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (335.29, 156.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 398, 609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1449) [M::mem_pestat] mean and std.dev: (344.96, 225.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1869) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680756 reads in 173.240 CPU sec, 5.625 real sec sorting block 52, #reads = 1075607 mem = 536877604 peak = 1017032704 [M::process] read 680726 sequences (100000151 bp) in 1.881 CPU sec, 1.960 real sec... [M::mem_process_seqs] Processed 680634 reads in 115.511 CPU sec, 3.700 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 280805, 10, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 242, 558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1306) [M::mem_pestat] mean and std.dev: (336.12, 240.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 316, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (333.90, 155.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (375, 614, 792) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1626) [M::mem_pestat] mean and std.dev: (647.11, 363.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2102) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 272, 635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1499) [M::mem_pestat] mean and std.dev: (440.83, 379.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1958) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 53, #reads = 1074883 mem = 536878637 peak = 1029722112 [M::process] read 684190 sequences (100000212 bp) in 2.672 CPU sec, 2.956 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 280636, 10, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 215, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (262.75, 148.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 318, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (334.63, 156.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (541, 1126, 1270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2728) [M::mem_pestat] mean and std.dev: (790.00, 377.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3457) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 354, 567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1337) [M::mem_pestat] mean and std.dev: (369.64, 205.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1722) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680580 reads in 175.814 CPU sec, 5.846 real sec [M::mem_process_seqs] Processed 680726 reads in 119.271 CPU sec, 3.987 real sec [M::process] read 684192 sequences (100000264 bp) in 2.159 CPU sec, 2.291 real sec... sorting block 54, #reads = 1075313 mem = 536882204 peak = 1029722112 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282200, 11, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 241, 577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1351) [M::mem_pestat] mean and std.dev: (324.15, 221.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1738) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 301, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (321.54, 154.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 136, 684) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1976) [M::mem_pestat] mean and std.dev: (380.80, 557.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2622) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (130, 181, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (215.72, 118.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684058 sequences (100000016 bp) in 2.692 CPU sec, 2.839 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 281768, 12, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 234, 697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1741) [M::mem_pestat] mean and std.dev: (287.66, 201.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2263) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 300, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (321.63, 156.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (384, 390, 1381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3375) [M::mem_pestat] mean and std.dev: (866.00, 877.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4377) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 255, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (258.67, 64.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683640 sequences (100000065 bp) in 1.539 CPU sec, 1.548 real sec... [M::mem_process_seqs] Processed 684190 reads in 178.626 CPU sec, 5.729 real sec [M::mem_process_seqs] Processed 684192 reads in 116.093 CPU sec, 3.682 real sec sorting block 55, #reads = 1076102 mem = 536877732 peak = 1029722112 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 281380, 11, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 246, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (246.90, 114.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 302, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (323.19, 156.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (114, 580, 2268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6576) [M::mem_pestat] mean and std.dev: (1028.73, 976.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8730) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 352, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994) [M::mem_pestat] mean and std.dev: (364.94, 189.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1248) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683034 sequences (100000099 bp) in 2.647 CPU sec, 2.821 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 281733, 5, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 242, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1169) [M::mem_pestat] mean and std.dev: (324.43, 208.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 306, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (325.67, 156.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 351, 1037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2769) [M::mem_pestat] mean and std.dev: (589.16, 567.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3635) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683044 sequences (100000146 bp) in 1.695 CPU sec, 1.696 real sec... [M::mem_process_seqs] Processed 684058 reads in 173.737 CPU sec, 5.553 real sec [M::mem_process_seqs] Processed 683640 reads in 114.123 CPU sec, 3.631 real sec sorting block 56, #reads = 1079207 mem = 536882171 peak = 1029722112 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 281907, 5, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 276, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1230) [M::mem_pestat] mean and std.dev: (282.42, 152.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 308, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (327.30, 157.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 243, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (219.90, 57.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682534 sequences (100000018 bp) in 2.627 CPU sec, 2.794 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 281129, 8, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 218, 648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1588) [M::mem_pestat] mean and std.dev: (337.23, 354.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2058) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 310, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (328.69, 157.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 205, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1197) [M::mem_pestat] mean and std.dev: (267.06, 205.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1539) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682366 sequences (100000060 bp) in 1.648 CPU sec, 1.653 real sec... [M::mem_process_seqs] Processed 683034 reads in 172.656 CPU sec, 5.515 real sec sorting block 57, #reads = 1078775 mem = 536878067 peak = 1029722112 [M::mem_process_seqs] Processed 683044 reads in 115.236 CPU sec, 3.665 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 281108, 8, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 216, 1276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3516) [M::mem_pestat] mean and std.dev: (497.81, 554.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 311, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (329.88, 157.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 227, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977) [M::mem_pestat] mean and std.dev: (269.09, 150.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1245) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 58, #reads = 1077881 mem = 536883622 peak = 1084825600 [M::process] read 682190 sequences (100000225 bp) in 2.747 CPU sec, 2.912 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 280982, 5, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 356, 595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1491) [M::mem_pestat] mean and std.dev: (419.00, 288.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1939) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 312, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (330.15, 156.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 298, 835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2039) [M::mem_pestat] mean and std.dev: (474.34, 349.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2641) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682534 reads in 166.003 CPU sec, 5.301 real sec [M::process] read 681968 sequences (100000091 bp) in 1.886 CPU sec, 1.901 real sec... [M::mem_process_seqs] Processed 682366 reads in 108.934 CPU sec, 3.442 real sec sorting block 59, #reads = 1078899 mem = 536881749 peak = 1084825600 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 281648, 7, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 293, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1145) [M::mem_pestat] mean and std.dev: (303.21, 169.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 315, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (333.01, 158.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 338, 765) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1959) [M::mem_pestat] mean and std.dev: (478.91, 414.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2556) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681620 sequences (100000088 bp) in 2.652 CPU sec, 2.815 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281193, 13, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 209, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (227.00, 113.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 316, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (334.38, 159.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1299, 1616, 1622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (653, 2268) [M::mem_pestat] mean and std.dev: (1532.20, 155.80) [M::mem_pestat] low and high boundaries for proper pairs: (330, 2591) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 304, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (273.13, 80.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682190 reads in 163.421 CPU sec, 5.194 real sec [M::process] read 681728 sequences (100000245 bp) in 1.884 CPU sec, 1.888 real sec... [M::mem_process_seqs] Processed 681968 reads in 106.959 CPU sec, 3.378 real sec sorting block 60, #reads = 1077857 mem = 536877694 peak = 1084825600 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 280741, 8, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 293, 838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2138) [M::mem_pestat] mean and std.dev: (583.52, 550.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2788) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 319, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (336.73, 160.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 248, 1031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2799) [M::mem_pestat] mean and std.dev: (502.03, 470.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3683) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681622 sequences (100000151 bp) in 2.717 CPU sec, 2.869 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 280470, 11, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 324, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (270.86, 108.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 317, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (336.39, 160.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (477, 894, 4259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11823) [M::mem_pestat] mean and std.dev: (2178.09, 1871.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15605) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 264, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1273) [M::mem_pestat] mean and std.dev: (370.82, 276.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1647) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681404 sequences (100000240 bp) in 1.567 CPU sec, 1.570 real sec... [M::mem_process_seqs] Processed 681620 reads in 164.166 CPU sec, 5.221 real sec sorting block 61, #reads = 1078418 mem = 536880449 peak = 1084825600 [M::mem_process_seqs] Processed 681728 reads in 109.408 CPU sec, 3.469 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 281352, 9, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 300, 552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1290) [M::mem_pestat] mean and std.dev: (418.02, 309.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 320, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923) [M::mem_pestat] mean and std.dev: (338.60, 161.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (121, 191, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (221.94, 142.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1021) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 62, #reads = 1075955 mem = 536880584 peak = 1084825600 [M::process] read 681786 sequences (100000264 bp) in 2.856 CPU sec, 3.028 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 280794, 7, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 364, 1651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4615) [M::mem_pestat] mean and std.dev: (624.90, 644.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6097) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 318, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (338.15, 162.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 279, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (265.14, 88.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682086 sequences (100000253 bp) in 1.785 CPU sec, 1.789 real sec... [M::mem_process_seqs] Processed 681622 reads in 164.160 CPU sec, 5.213 real sec [M::mem_process_seqs] Processed 681404 reads in 109.175 CPU sec, 3.459 real sec sorting block 63, #reads = 1076211 mem = 536878993 peak = 1084825600 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 281332, 5, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 224, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008) [M::mem_pestat] mean and std.dev: (247.00, 128.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1288) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 317, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (335.62, 159.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (123, 234, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1107) [M::mem_pestat] mean and std.dev: (298.55, 244.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1435) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681956 sequences (100000247 bp) in 2.883 CPU sec, 3.032 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281421, 7, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 245, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1142) [M::mem_pestat] mean and std.dev: (251.40, 117.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 313, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (333.20, 159.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1138) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 327, 933) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2441) [M::mem_pestat] mean and std.dev: (546.95, 490.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3195) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681786 reads in 162.958 CPU sec, 5.167 real sec [M::process] read 682142 sequences (100000025 bp) in 1.830 CPU sec, 1.830 real sec... [M::mem_process_seqs] Processed 682086 reads in 106.392 CPU sec, 3.358 real sec sorting block 64, #reads = 1076535 mem = 536878499 peak = 1084825600 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 281492, 7, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 200, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799) [M::mem_pestat] mean and std.dev: (251.21, 138.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 312, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (332.24, 158.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 325, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (320.67, 136.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682132 sequences (100000238 bp) in 2.828 CPU sec, 2.967 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 280363, 10, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 189, 849) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2209) [M::mem_pestat] mean and std.dev: (308.18, 307.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2889) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 311, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (331.58, 159.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (425, 1029, 1159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2627) [M::mem_pestat] mean and std.dev: (745.00, 378.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3361) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 285, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (337.13, 202.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682082 sequences (100000272 bp) in 1.923 CPU sec, 1.931 real sec... [M::mem_process_seqs] Processed 681956 reads in 166.896 CPU sec, 5.292 real sec [M::mem_process_seqs] Processed 682142 reads in 112.086 CPU sec, 3.550 real sec sorting block 65, #reads = 1076863 mem = 536878384 peak = 1084825600 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 281636, 5, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 303, 694) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1714) [M::mem_pestat] mean and std.dev: (451.78, 365.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2224) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 309, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (329.45, 157.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (136, 252, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (252.85, 170.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1200) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681878 sequences (100000174 bp) in 2.958 CPU sec, 3.141 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 280577, 6, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 245, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (236.57, 93.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 310, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (329.54, 157.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 229, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (259.46, 107.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682132 reads in 173.614 CPU sec, 5.530 real sec [M::process] read 682066 sequences (100000276 bp) in 1.822 CPU sec, 1.826 real sec... sorting block 66, #reads = 1076686 mem = 536880144 peak = 1084825600 [M::mem_process_seqs] Processed 682082 reads in 114.955 CPU sec, 3.637 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 280857, 5, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 234, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (245.81, 97.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 310, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (329.83, 157.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 265, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (274.54, 130.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 67, #reads = 1076285 mem = 536879506 peak = 1084825600 [M::process] read 681684 sequences (100000183 bp) in 3.018 CPU sec, 3.178 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 281198, 13, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 313, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1315) [M::mem_pestat] mean and std.dev: (300.85, 166.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1696) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 309, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (328.36, 155.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (454, 454, 802) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1498) [M::mem_pestat] mean and std.dev: (511.69, 195.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1846) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 340, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996) [M::mem_pestat] mean and std.dev: (298.56, 151.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1263) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681878 reads in 171.009 CPU sec, 5.447 real sec [M::process] read 681480 sequences (100000057 bp) in 1.921 CPU sec, 1.947 real sec... [M::mem_process_seqs] Processed 682066 reads in 113.329 CPU sec, 3.580 real sec sorting block 68, #reads = 1076009 mem = 536880446 peak = 1084825600 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 280964, 4, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 244, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (249.90, 97.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1096) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 309, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (327.85, 154.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 212, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (233.11, 100.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681274 sequences (100000202 bp) in 2.828 CPU sec, 2.983 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 280875, 9, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 248, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1255) [M::mem_pestat] mean and std.dev: (389.43, 316.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 312, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (330.66, 155.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 366, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (346.36, 155.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681684 reads in 167.532 CPU sec, 5.328 real sec [M::process] read 681366 sequences (100000162 bp) in 1.864 CPU sec, 1.867 real sec... [M::mem_process_seqs] Processed 681480 reads in 111.032 CPU sec, 3.511 real sec sorting block 69, #reads = 1076988 mem = 536883076 peak = 1084825600 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 281132, 11, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 355, 620) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1476) [M::mem_pestat] mean and std.dev: (370.22, 233.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1904) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 313, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (331.50, 155.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (75, 901, 2473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7269) [M::mem_pestat] mean and std.dev: (1017.09, 1114.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9667) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 302, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (291.33, 120.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681072 sequences (100000224 bp) in 2.998 CPU sec, 3.149 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 281142, 7, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 335, 764) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1846) [M::mem_pestat] mean and std.dev: (409.69, 294.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2387) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 314, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (332.61, 156.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 287, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (248.60, 90.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681274 reads in 167.173 CPU sec, 5.307 real sec [M::process] read 681176 sequences (100000296 bp) in 1.971 CPU sec, 2.005 real sec... [M::mem_process_seqs] Processed 681366 reads in 111.578 CPU sec, 3.538 real sec sorting block 70, #reads = 1076131 mem = 536881035 peak = 1084825600 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 281115, 12, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 229, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (213.45, 98.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 316, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (334.25, 157.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (351, 518, 7343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 21327) [M::mem_pestat] mean and std.dev: (3270.75, 3442.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 28319) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 371, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1129) [M::mem_pestat] mean and std.dev: (343.54, 200.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1427) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680858 sequences (100000209 bp) in 2.845 CPU sec, 3.007 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 280803, 13, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 291, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (278.38, 131.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1186) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 316, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (333.74, 157.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1268, 1912, 9143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 24893) [M::mem_pestat] mean and std.dev: (3408.31, 3253.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 32768) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 286, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (326.59, 158.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1097) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681072 reads in 167.958 CPU sec, 5.369 real sec sorting block 71, #reads = 1077212 mem = 536879088 peak = 1084825600 [M::process] read 685636 sequences (100000164 bp) in 1.990 CPU sec, 2.029 real sec... [M::mem_process_seqs] Processed 681176 reads in 112.706 CPU sec, 3.551 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 280681, 4, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 286, 562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1380) [M::mem_pestat] mean and std.dev: (305.81, 221.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 316, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (334.84, 157.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (262, 392, 1974) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5398) [M::mem_pestat] mean and std.dev: (778.42, 805.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7110) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 72, #reads = 1076754 mem = 536880649 peak = 1084825600 [M::process] read 687464 sequences (100000174 bp) in 3.333 CPU sec, 3.530 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 282501, 4, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 338, 698) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1804) [M::mem_pestat] mean and std.dev: (420.56, 400.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2357) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 293, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (317.49, 157.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 253, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1200) [M::mem_pestat] mean and std.dev: (298.92, 197.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1548) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680858 reads in 177.788 CPU sec, 5.655 real sec [M::mem_process_seqs] Processed 685636 reads in 123.162 CPU sec, 3.881 real sec [M::process] read 687046 sequences (100000160 bp) in 2.839 CPU sec, 2.949 real sec... sorting block 73, #reads = 1074815 mem = 536880010 peak = 1084825600 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 283306, 1, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 230, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016) [M::mem_pestat] mean and std.dev: (281.21, 189.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1294) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 282, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (309.38, 155.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1085) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 244, 676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1686) [M::mem_pestat] mean and std.dev: (312.37, 197.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2191) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686026 sequences (100000051 bp) in 3.126 CPU sec, 3.340 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 283274, 2, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 324, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (268.14, 128.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 286, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (312.29, 156.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1096) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 372, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1059) [M::mem_pestat] mean and std.dev: (311.31, 144.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1344) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687464 reads in 183.612 CPU sec, 5.852 real sec [M::mem_process_seqs] Processed 687046 reads in 120.167 CPU sec, 3.791 real sec [M::process] read 685490 sequences (100000155 bp) in 2.270 CPU sec, 2.365 real sec... sorting block 74, #reads = 1074848 mem = 536879885 peak = 1084825600 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 283274, 6, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 221, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (246.56, 113.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1030) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 291, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (317.02, 157.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 223, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (256.90, 115.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685428 sequences (100000241 bp) in 2.954 CPU sec, 3.115 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282540, 10, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 272, 719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1835) [M::mem_pestat] mean and std.dev: (402.95, 372.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2393) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 294, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (319.39, 157.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1001, 4837, 4837) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12509) [M::mem_pestat] mean and std.dev: (3600.50, 1895.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16345) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 262, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (263.49, 97.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686026 reads in 176.339 CPU sec, 5.601 real sec [M::process] read 686536 sequences (100000273 bp) in 1.991 CPU sec, 2.033 real sec... sorting block 75, #reads = 1077531 mem = 536880726 peak = 1084825600 [M::mem_process_seqs] Processed 685490 reads in 115.503 CPU sec, 3.638 real sec sorting block 76, #reads = 1077956 mem = 536882435 peak = 1084825600 [M::process] read 684746 sequences (100000253 bp) in 2.715 CPU sec, 2.862 real sec... [M::process] read 684276 sequences (100000023 bp) in 1.679 CPU sec, 1.685 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282940, 10, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 255, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (260.51, 162.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1185) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 284, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (310.13, 154.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (469, 469, 1135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2467) [M::mem_pestat] mean and std.dev: (703.11, 487.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3133) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 318, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014) [M::mem_pestat] mean and std.dev: (311.76, 183.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1297) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686536 reads in 100.454 CPU sec, 3.174 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 282998, 16, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 369, 1142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3032) [M::mem_pestat] mean and std.dev: (665.86, 823.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3977) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 295, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (320.46, 158.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (398, 1111, 1134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2606) [M::mem_pestat] mean and std.dev: (885.67, 417.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3342) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 323, 574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1290) [M::mem_pestat] mean and std.dev: (395.45, 224.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1648) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 282177, 9, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 276, 597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1441) [M::mem_pestat] mean and std.dev: (335.20, 247.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1863) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 300, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (323.03, 158.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 229, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (217.90, 77.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685428 reads in 275.997 CPU sec, 8.765 real sec [M::process] read 683634 sequences (100000243 bp) in 2.273 CPU sec, 2.293 real sec... sorting block 77, #reads = 1078296 mem = 536879151 peak = 1084825600 [M::mem_process_seqs] Processed 684746 reads in 134.224 CPU sec, 4.277 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 282507, 10, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 255, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1071) [M::mem_pestat] mean and std.dev: (331.46, 169.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1365) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 303, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (326.00, 159.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (450, 1239, 2058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5274) [M::mem_pestat] mean and std.dev: (1329.70, 1098.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6882) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 322, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1092) [M::mem_pestat] mean and std.dev: (330.84, 244.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1403) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 78, #reads = 1077967 mem = 536877846 peak = 1084825600 [M::process] read 683488 sequences (100000263 bp) in 2.688 CPU sec, 2.859 real sec... [M::process] read 683588 sequences (100000213 bp) in 1.620 CPU sec, 1.620 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 281976, 5, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 263, 846) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2226) [M::mem_pestat] mean and std.dev: (482.75, 508.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2916) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 307, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (328.19, 159.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 326, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955) [M::mem_pestat] mean and std.dev: (287.47, 148.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1221) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684276 reads in 180.978 CPU sec, 5.758 real sec [M::mem_process_seqs] Processed 683634 reads in 117.649 CPU sec, 3.743 real sec sorting block 79, #reads = 1076593 mem = 536880308 peak = 1084825600 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 281748, 9, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 353, 713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1649) [M::mem_pestat] mean and std.dev: (393.12, 260.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2117) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 309, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (329.94, 159.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (253, 344, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (27, 592) [M::mem_pestat] mean and std.dev: (297.67, 94.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684542 sequences (100000219 bp) in 2.560 CPU sec, 2.723 real sec... [M::process] read 683900 sequences (100000169 bp) in 1.890 CPU sec, 1.898 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 281704, 10, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 404, 702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1666) [M::mem_pestat] mean and std.dev: (356.21, 188.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2148) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 308, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (329.01, 160.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1155) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (213, 1176, 1176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3102) [M::mem_pestat] mean and std.dev: (976.60, 676.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4065) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 341, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (293.00, 147.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1006) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683488 reads in 181.443 CPU sec, 5.755 real sec [M::mem_process_seqs] Processed 683588 reads in 115.969 CPU sec, 3.673 real sec sorting block 80, #reads = 1074298 mem = 536883965 peak = 1084825600 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282595, 10, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 205, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062) [M::mem_pestat] mean and std.dev: (238.83, 131.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1358) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 299, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (322.38, 157.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1997, 2245, 2291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1409, 2879) [M::mem_pestat] mean and std.dev: (2270.12, 153.35) [M::mem_pestat] low and high boundaries for proper pairs: (1115, 3173) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 412, 999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2633) [M::mem_pestat] mean and std.dev: (673.39, 668.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3450) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683016 sequences (100000120 bp) in 2.826 CPU sec, 2.976 real sec... [M::process] read 683272 sequences (100000040 bp) in 1.564 CPU sec, 1.563 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282215, 7, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (258, 321, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (320.06, 129.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 301, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (323.56, 156.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 370, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (305.23, 114.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684542 reads in 180.022 CPU sec, 5.724 real sec sorting block 81, #reads = 1075300 mem = 536878420 peak = 1084825600 [M::mem_process_seqs] Processed 683900 reads in 119.392 CPU sec, 3.798 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281914, 5, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 232, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (312.12, 245.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1293) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 314, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (333.67, 161.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1167) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 210, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018) [M::mem_pestat] mean and std.dev: (273.94, 148.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1294) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682340 sequences (100000042 bp) in 2.499 CPU sec, 2.666 real sec... [M::process] read 682046 sequences (100000125 bp) in 1.660 CPU sec, 1.663 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282085, 7, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 238, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (243.10, 99.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 311, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (330.87, 159.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 271, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (256.59, 101.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1084) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683016 reads in 180.734 CPU sec, 5.753 real sec sorting block 82, #reads = 1074857 mem = 536880557 peak = 1084825600 [M::mem_process_seqs] Processed 683272 reads in 117.209 CPU sec, 3.722 real sec sorting block 83, #reads = 1075502 mem = 536876744 peak = 1101115392 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 281697, 6, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 299, 661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1657) [M::mem_pestat] mean and std.dev: (412.59, 287.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2155) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 317, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931) [M::mem_pestat] mean and std.dev: (335.57, 160.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1173) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 338, 632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1436) [M::mem_pestat] mean and std.dev: (374.40, 207.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1838) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681438 sequences (100000150 bp) in 2.578 CPU sec, 2.734 real sec... [M::process] read 681468 sequences (100000178 bp) in 1.743 CPU sec, 1.746 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 281115, 6, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 398, 933) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2357) [M::mem_pestat] mean and std.dev: (667.71, 642.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3236) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 316, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (331.72, 155.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 302, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (284.33, 127.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682340 reads in 182.818 CPU sec, 5.824 real sec [M::mem_process_seqs] Processed 682046 reads in 120.059 CPU sec, 3.815 real sec sorting block 84, #reads = 1073066 mem = 536879237 peak = 1101115392 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 281423, 4, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 246, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974) [M::mem_pestat] mean and std.dev: (293.57, 162.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1245) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 319, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (335.65, 157.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 292, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (251.03, 119.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1189) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680926 sequences (100000116 bp) in 2.493 CPU sec, 2.676 real sec... [M::process] read 680382 sequences (100000231 bp) in 1.650 CPU sec, 1.643 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 281026, 7, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 327, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (243.52, 108.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 318, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (334.68, 156.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 290, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (294.95, 163.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681438 reads in 184.558 CPU sec, 5.883 real sec [M::mem_process_seqs] Processed 681468 reads in 122.861 CPU sec, 3.912 real sec sorting block 85, #reads = 1073137 mem = 536877150 peak = 1101115392 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 280979, 18, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (238, 350, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (331.61, 168.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 319, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (336.28, 157.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1142) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (490, 594, 2463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6409) [M::mem_pestat] mean and std.dev: (762.64, 666.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8382) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 258, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985) [M::mem_pestat] mean and std.dev: (323.21, 153.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1240) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680680 sequences (100000185 bp) in 3.091 CPU sec, 3.242 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 280876, 4, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 259, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (252.37, 91.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 321, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (338.17, 158.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (246, 607, 695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1593) [M::mem_pestat] mean and std.dev: (467.42, 323.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2042) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680872 sequences (100000000 bp) in 1.922 CPU sec, 1.926 real sec... [M::mem_process_seqs] Processed 680926 reads in 182.386 CPU sec, 5.790 real sec sorting block 86, #reads = 1072098 mem = 536879512 peak = 1101115392 [M::mem_process_seqs] Processed 680382 reads in 120.156 CPU sec, 3.805 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 281061, 5, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 315, 629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1627) [M::mem_pestat] mean and std.dev: (382.17, 369.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2126) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 318, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (335.95, 157.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1138) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 221, 631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1589) [M::mem_pestat] mean and std.dev: (356.34, 257.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2068) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680864 sequences (100000281 bp) in 2.358 CPU sec, 2.481 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 281011, 4, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 250, 595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1413) [M::mem_pestat] mean and std.dev: (343.11, 267.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1822) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 320, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (337.53, 158.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (244, 377, 815) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1957) [M::mem_pestat] mean and std.dev: (493.78, 301.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2528) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681030 sequences (100000051 bp) in 1.970 CPU sec, 1.966 real sec... [M::mem_process_seqs] Processed 680680 reads in 184.048 CPU sec, 5.868 real sec sorting block 87, #reads = 1072071 mem = 536880955 peak = 1101115392 [M::mem_process_seqs] Processed 680872 reads in 119.123 CPU sec, 3.773 real sec sorting block 88, #reads = 1071867 mem = 536878624 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 281373, 4, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 299, 1106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2844) [M::mem_pestat] mean and std.dev: (626.33, 691.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 321, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (338.08, 158.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (281, 331, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992) [M::mem_pestat] mean and std.dev: (368.74, 166.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1229) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686026 sequences (100000111 bp) in 3.157 CPU sec, 3.291 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 280815, 5, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 385, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1159) [M::mem_pestat] mean and std.dev: (369.94, 192.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 321, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (339.52, 160.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1159) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 290, 573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1329) [M::mem_pestat] mean and std.dev: (303.74, 164.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1707) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689552 sequences (100000133 bp) in 1.977 CPU sec, 1.979 real sec... [M::mem_process_seqs] Processed 680864 reads in 181.853 CPU sec, 5.768 real sec [M::mem_process_seqs] Processed 681030 reads in 120.927 CPU sec, 3.821 real sec sorting block 89, #reads = 1071777 mem = 536879788 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 282956, 7, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 204, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841) [M::mem_pestat] mean and std.dev: (299.00, 206.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 292, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (315.93, 154.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 291, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (308.41, 155.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689736 sequences (100000247 bp) in 2.877 CPU sec, 3.020 real sec... [M::process] read 688056 sequences (100000246 bp) in 1.770 CPU sec, 1.774 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 284357, 2, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 221, 555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1371) [M::mem_pestat] mean and std.dev: (276.45, 198.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1779) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 272, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (296.79, 145.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 346, 705) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1701) [M::mem_pestat] mean and std.dev: (423.48, 317.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2199) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686026 reads in 196.577 CPU sec, 6.252 real sec [M::mem_process_seqs] Processed 689552 reads in 128.270 CPU sec, 4.074 real sec sorting block 90, #reads = 1070674 mem = 536878868 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 284497, 9, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 216, 660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1690) [M::mem_pestat] mean and std.dev: (411.63, 442.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2205) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 271, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (294.98, 143.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 247, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (261.44, 113.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687020 sequences (100000073 bp) in 3.380 CPU sec, 3.527 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 284039, 15, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 260, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (277.88, 133.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1070) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 280, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (301.77, 145.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (471, 2724, 2780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7398) [M::mem_pestat] mean and std.dev: (1755.07, 1193.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9707) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 218, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014) [M::mem_pestat] mean and std.dev: (292.57, 176.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1287) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686484 sequences (100000271 bp) in 2.093 CPU sec, 2.101 real sec... [M::mem_process_seqs] Processed 689736 reads in 200.068 CPU sec, 6.346 real sec sorting block 91, #reads = 1072908 mem = 536879737 peak = 1144324096 [M::mem_process_seqs] Processed 688056 reads in 129.605 CPU sec, 4.102 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 283299, 25, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 225, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (236.41, 101.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 285, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848) [M::mem_pestat] mean and std.dev: (307.55, 148.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1068) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (452, 624, 1896) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4784) [M::mem_pestat] mean and std.dev: (1162.43, 1059.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6228) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 305, 617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1519) [M::mem_pestat] mean and std.dev: (446.03, 328.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1970) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 92, #reads = 1070103 mem = 536886675 peak = 1144324096 [M::process] read 685300 sequences (100000194 bp) in 2.640 CPU sec, 2.786 real sec... [M::process] read 684516 sequences (100000012 bp) in 1.635 CPU sec, 1.642 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282976, 7, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 213, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1180) [M::mem_pestat] mean and std.dev: (349.53, 249.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 288, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (310.27, 150.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1081) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 335, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (333.14, 210.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1178) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687020 reads in 191.098 CPU sec, 6.099 real sec [M::mem_process_seqs] Processed 686484 reads in 125.792 CPU sec, 3.997 real sec sorting block 93, #reads = 1073194 mem = 536881685 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 282984, 5, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 362, 1052) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2716) [M::mem_pestat] mean and std.dev: (508.06, 469.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 296, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (316.15, 152.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (271, 465, 681) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1501) [M::mem_pestat] mean and std.dev: (467.31, 298.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1911) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684604 sequences (100000300 bp) in 3.009 CPU sec, 3.142 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282539, 14, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 522, 573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1315) [M::mem_pestat] mean and std.dev: (345.25, 220.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 300, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (320.85, 154.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (154, 483, 1180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3232) [M::mem_pestat] mean and std.dev: (788.86, 805.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4258) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 232, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1097) [M::mem_pestat] mean and std.dev: (260.88, 160.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1402) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684066 sequences (100000188 bp) in 2.047 CPU sec, 2.053 real sec... [M::mem_process_seqs] Processed 685300 reads in 193.250 CPU sec, 6.138 real sec [M::mem_process_seqs] Processed 684516 reads in 125.768 CPU sec, 3.975 real sec sorting block 94, #reads = 1072724 mem = 536879097 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 282844, 5, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 240, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (256.90, 162.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 302, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (321.99, 154.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 433, 1251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3307) [M::mem_pestat] mean and std.dev: (483.30, 443.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4335) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684288 sequences (100000106 bp) in 2.839 CPU sec, 2.974 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 282283, 11, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 322, 499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1137) [M::mem_pestat] mean and std.dev: (315.83, 180.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 303, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (323.40, 155.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 1822, 2258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6708) [M::mem_pestat] mean and std.dev: (1157.50, 1032.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8933) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 403, 680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1604) [M::mem_pestat] mean and std.dev: (389.00, 211.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2066) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683682 sequences (100000222 bp) in 1.981 CPU sec, 1.986 real sec... [M::mem_process_seqs] Processed 684604 reads in 193.427 CPU sec, 6.145 real sec sorting block 95, #reads = 1073121 mem = 536887574 peak = 1144324096 [M::mem_process_seqs] Processed 684066 reads in 133.524 CPU sec, 4.228 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282804, 11, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 255, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (243.06, 99.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 303, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (322.33, 154.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (876, 1317, 2587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6009) [M::mem_pestat] mean and std.dev: (1960.27, 1564.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8218) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 393, 626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1420) [M::mem_pestat] mean and std.dev: (429.56, 263.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1817) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683862 sequences (100000283 bp) in 2.999 CPU sec, 3.135 real sec... [M::process] read 683250 sequences (100000088 bp) in 1.935 CPU sec, 1.945 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 282013, 14, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 305, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1269) [M::mem_pestat] mean and std.dev: (352.68, 241.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 306, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (324.19, 154.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (409, 595, 966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2080) [M::mem_pestat] mean and std.dev: (750.36, 539.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2909) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 306, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (277.62, 100.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684288 reads in 191.350 CPU sec, 6.073 real sec sorting block 96, #reads = 1071154 mem = 536881063 peak = 1144324096 [M::mem_process_seqs] Processed 683682 reads in 122.839 CPU sec, 3.895 real sec sorting block 97, #reads = 1071791 mem = 536883959 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 282738, 8, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 387, 560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1290) [M::mem_pestat] mean and std.dev: (308.06, 144.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 303, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (322.73, 153.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (250, 364, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (291.62, 89.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683016 sequences (100000280 bp) in 2.907 CPU sec, 3.043 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281666, 12, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 218, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (229.19, 85.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 308, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (327.15, 155.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1235, 1235, 2045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3665) [M::mem_pestat] mean and std.dev: (1465.09, 512.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4475) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 189, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (225.79, 112.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683250 sequences (100000270 bp) in 1.977 CPU sec, 1.978 real sec... [M::mem_process_seqs] Processed 683862 reads in 189.266 CPU sec, 6.019 real sec [M::mem_process_seqs] Processed 683250 reads in 124.907 CPU sec, 3.951 real sec sorting block 98, #reads = 1073834 mem = 536877932 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282140, 22, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 274, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (62, 482) [M::mem_pestat] mean and std.dev: (246.23, 71.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 310, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (328.38, 156.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (526, 575, 1142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2374) [M::mem_pestat] mean and std.dev: (744.62, 418.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2990) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 220, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799) [M::mem_pestat] mean and std.dev: (258.85, 128.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1016) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682646 sequences (100000184 bp) in 2.412 CPU sec, 2.539 real sec... [M::process] read 681886 sequences (100000171 bp) in 1.840 CPU sec, 1.844 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 281901, 9, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 254, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (229.68, 92.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 308, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (327.64, 156.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 293, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (291.63, 133.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1080) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683016 reads in 185.151 CPU sec, 5.895 real sec [M::mem_process_seqs] Processed 683250 reads in 121.673 CPU sec, 3.867 real sec sorting block 99, #reads = 1072141 mem = 536879097 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 281195, 20, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 179, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1174) [M::mem_pestat] mean and std.dev: (314.54, 224.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 313, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (331.63, 158.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1155) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (312, 515, 1803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4785) [M::mem_pestat] mean and std.dev: (596.18, 549.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6276) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 424, 915) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2293) [M::mem_pestat] mean and std.dev: (572.13, 508.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2982) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681426 sequences (100000013 bp) in 2.291 CPU sec, 2.405 real sec... [M::process] read 681184 sequences (100000069 bp) in 1.630 CPU sec, 1.630 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 281063, 6, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (252, 439, 894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2178) [M::mem_pestat] mean and std.dev: (626.78, 441.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2820) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 318, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (335.73, 159.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 226, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (242.56, 91.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682646 reads in 180.756 CPU sec, 5.770 real sec sorting block 100, #reads = 1073242 mem = 536882475 peak = 1144324096 [M::mem_process_seqs] Processed 681886 reads in 117.506 CPU sec, 3.738 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 280773, 21, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 286, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (256.92, 126.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 321, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932) [M::mem_pestat] mean and std.dev: (339.26, 160.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1173) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (354, 354, 1788) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4656) [M::mem_pestat] mean and std.dev: (848.24, 758.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6090) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 232, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 468) [M::mem_pestat] mean and std.dev: (234.46, 74.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 101, #reads = 1073091 mem = 536879324 peak = 1144324096 [M::process] read 681598 sequences (100000190 bp) in 3.264 CPU sec, 3.402 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 280686, 11, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 357, 618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1434) [M::mem_pestat] mean and std.dev: (432.47, 266.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1842) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 321, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (339.34, 160.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1170) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (306, 435, 783) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1737) [M::mem_pestat] mean and std.dev: (442.90, 227.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2214) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 304, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (281.84, 106.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681426 reads in 182.936 CPU sec, 5.807 real sec [M::process] read 681790 sequences (100000023 bp) in 2.081 CPU sec, 2.086 real sec... [M::mem_process_seqs] Processed 681184 reads in 123.603 CPU sec, 3.903 real sec sorting block 102, #reads = 1072929 mem = 536878736 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 281328, 10, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 353, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1203) [M::mem_pestat] mean and std.dev: (318.88, 152.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 318, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (338.05, 161.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1168) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 976, 4178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12512) [M::mem_pestat] mean and std.dev: (1661.50, 1801.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16679) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 247, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031) [M::mem_pestat] mean and std.dev: (298.49, 165.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1312) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681340 sequences (100000063 bp) in 2.952 CPU sec, 3.094 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 281242, 4, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 241, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (250.50, 86.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 318, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931) [M::mem_pestat] mean and std.dev: (338.55, 163.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (258, 346, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1113) [M::mem_pestat] mean and std.dev: (337.86, 143.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1398) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682302 sequences (100000232 bp) in 2.026 CPU sec, 2.027 real sec... [M::mem_process_seqs] Processed 681598 reads in 180.522 CPU sec, 5.729 real sec [M::mem_process_seqs] Processed 681790 reads in 121.197 CPU sec, 3.829 real sec sorting block 103, #reads = 1072482 mem = 536877543 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 281074, 2, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 282, 669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1635) [M::mem_pestat] mean and std.dev: (388.91, 335.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2118) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 318, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (337.74, 161.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1164) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (250, 352, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (329.15, 94.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681724 sequences (100000063 bp) in 3.068 CPU sec, 3.203 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 281590, 8, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 346, 1646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4600) [M::mem_pestat] mean and std.dev: (1064.93, 1233.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6077) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 310, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (332.88, 161.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (245, 636, 1820) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4970) [M::mem_pestat] mean and std.dev: (925.96, 895.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6545) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686774 sequences (100000139 bp) in 2.031 CPU sec, 2.038 real sec... [M::mem_process_seqs] Processed 681340 reads in 185.930 CPU sec, 5.907 real sec [M::mem_process_seqs] Processed 682302 reads in 123.382 CPU sec, 3.895 real sec sorting block 104, #reads = 1071810 mem = 536878116 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 280997, 13, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 267, 649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1641) [M::mem_pestat] mean and std.dev: (452.96, 461.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2297) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 316, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932) [M::mem_pestat] mean and std.dev: (337.66, 163.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (175, 342, 2231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6343) [M::mem_pestat] mean and std.dev: (704.08, 840.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8399) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 383, 562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1268) [M::mem_pestat] mean and std.dev: (399.43, 263.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1621) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687496 sequences (100000099 bp) in 2.949 CPU sec, 3.079 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282747, 9, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 290, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (271.50, 129.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 287, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (310.76, 152.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1084) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 231, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (254.25, 122.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686896 sequences (100000210 bp) in 2.100 CPU sec, 2.101 real sec... [M::mem_process_seqs] Processed 681724 reads in 208.054 CPU sec, 6.626 real sec sorting block 105, #reads = 1071383 mem = 536879877 peak = 1144324096 [M::mem_process_seqs] Processed 686774 reads in 136.742 CPU sec, 4.330 real sec sorting block 106, #reads = 1064383 mem = 536877489 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283980, 9, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 192, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972) [M::mem_pestat] mean and std.dev: (240.58, 150.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1243) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 282, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846) [M::mem_pestat] mean and std.dev: (306.33, 150.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1066) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 300, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (265.56, 103.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685692 sequences (100000235 bp) in 3.124 CPU sec, 3.236 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 283101, 11, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 176, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (268.26, 228.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1181) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 286, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (309.83, 152.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1080) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1924, 1924, 2329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1114, 3139) [M::mem_pestat] mean and std.dev: (1821.12, 322.75) [M::mem_pestat] low and high boundaries for proper pairs: (530, 3544) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 218, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (232.62, 104.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687496 reads in 168.385 CPU sec, 5.323 real sec [M::process] read 685186 sequences (100000184 bp) in 1.960 CPU sec, 1.966 real sec... [M::mem_process_seqs] Processed 686896 reads in 107.505 CPU sec, 3.390 real sec sorting block 107, #reads = 1076322 mem = 536878188 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282474, 5, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 268, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1061) [M::mem_pestat] mean and std.dev: (309.36, 211.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1369) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 293, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (314.76, 153.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1088) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 253, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (280.03, 153.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684610 sequences (100000087 bp) in 2.822 CPU sec, 2.949 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282793, 7, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 184, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (218.72, 117.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 298, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (318.85, 154.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 288, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (263.38, 105.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684520 sequences (100000158 bp) in 1.620 CPU sec, 1.611 real sec... [M::mem_process_seqs] Processed 685692 reads in 169.724 CPU sec, 5.395 real sec [M::mem_process_seqs] Processed 685186 reads in 112.555 CPU sec, 3.561 real sec sorting block 108, #reads = 1077555 mem = 536880452 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282539, 7, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 239, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (244.25, 94.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 299, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (320.00, 154.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 204, 622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1538) [M::mem_pestat] mean and std.dev: (379.35, 382.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1996) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684088 sequences (100000077 bp) in 3.383 CPU sec, 3.536 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282804, 3, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 370, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (340.69, 168.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 301, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (322.35, 155.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 287, 692) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1724) [M::mem_pestat] mean and std.dev: (355.96, 337.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2240) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684610 reads in 173.001 CPU sec, 5.504 real sec [M::process] read 683504 sequences (100000194 bp) in 1.631 CPU sec, 1.648 real sec... [M::mem_process_seqs] Processed 684520 reads in 111.469 CPU sec, 3.525 real sec sorting block 109, #reads = 1077276 mem = 536877781 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282115, 5, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 154, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (182.88, 96.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 304, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (324.40, 156.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 341, 729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1817) [M::mem_pestat] mean and std.dev: (419.87, 299.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2361) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 110, #reads = 1077123 mem = 536877776 peak = 1144324096 [M::process] read 683538 sequences (100000185 bp) in 2.610 CPU sec, 2.712 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 281834, 11, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2127) [M::mem_pestat] mean and std.dev: (462.31, 581.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2790) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 307, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (326.55, 156.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (445, 1228, 8344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 24142) [M::mem_pestat] mean and std.dev: (3530.36, 3704.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 32041) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 261, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005) [M::mem_pestat] mean and std.dev: (302.57, 133.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1280) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684088 reads in 162.146 CPU sec, 5.141 real sec [M::process] read 683020 sequences (100000212 bp) in 1.858 CPU sec, 1.866 real sec... [M::mem_process_seqs] Processed 683504 reads in 105.240 CPU sec, 3.316 real sec sorting block 111, #reads = 1076028 mem = 536878145 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282094, 9, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 284, 929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2441) [M::mem_pestat] mean and std.dev: (436.62, 357.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3197) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 307, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (326.08, 155.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (276, 374, 624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1320) [M::mem_pestat] mean and std.dev: (449.32, 257.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1668) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683370 sequences (100000201 bp) in 2.771 CPU sec, 2.888 real sec... [M::process] read 682936 sequences (100000162 bp) in 1.460 CPU sec, 1.463 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 281684, 7, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 251, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (237.63, 76.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 310, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (328.81, 157.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1138) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 242, 566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1308) [M::mem_pestat] mean and std.dev: (344.79, 213.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1679) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683538 reads in 162.542 CPU sec, 5.163 real sec [M::mem_process_seqs] Processed 683020 reads in 107.293 CPU sec, 3.400 real sec sorting block 112, #reads = 1075189 mem = 536877653 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282069, 12, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 305, 1512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4198) [M::mem_pestat] mean and std.dev: (710.15, 758.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 308, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (328.28, 157.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 637, 720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2084) [M::mem_pestat] mean and std.dev: (477.00, 433.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2766) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 310, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1156) [M::mem_pestat] mean and std.dev: (305.00, 176.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1486) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682774 sequences (100000253 bp) in 2.338 CPU sec, 2.444 real sec... [M::process] read 682534 sequences (100000197 bp) in 1.490 CPU sec, 1.495 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 281270, 4, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 217, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (217.56, 109.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 311, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (330.36, 158.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1150) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 276, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (270.88, 83.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683370 reads in 165.094 CPU sec, 5.251 real sec [M::mem_process_seqs] Processed 682936 reads in 104.305 CPU sec, 3.314 real sec sorting block 113, #reads = 1074682 mem = 536878254 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 281894, 5, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 407, 1244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3324) [M::mem_pestat] mean and std.dev: (813.63, 919.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 312, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (331.68, 159.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1151) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 294, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (275.20, 113.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682396 sequences (100000022 bp) in 2.236 CPU sec, 2.329 real sec... [M::process] read 682196 sequences (100000181 bp) in 1.440 CPU sec, 1.439 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 281585, 19, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 315, 652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1636) [M::mem_pestat] mean and std.dev: (366.15, 288.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2128) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 313, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (332.92, 159.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (732, 1221, 1451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2889) [M::mem_pestat] mean and std.dev: (1048.84, 463.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3608) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 240, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837) [M::mem_pestat] mean and std.dev: (279.21, 164.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682774 reads in 163.657 CPU sec, 5.212 real sec sorting block 114, #reads = 1076121 mem = 536877801 peak = 1144324096 [M::mem_process_seqs] Processed 682534 reads in 106.868 CPU sec, 3.397 real sec sorting block 115, #reads = 1073971 mem = 536879819 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 281292, 8, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 359, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (293.71, 108.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1162) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 314, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (333.26, 159.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 305, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975) [M::mem_pestat] mean and std.dev: (285.21, 178.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1241) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682088 sequences (100000021 bp) in 2.175 CPU sec, 2.288 real sec... [M::process] read 682322 sequences (100000167 bp) in 1.450 CPU sec, 1.445 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 281488, 12, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 313, 506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1216) [M::mem_pestat] mean and std.dev: (293.94, 162.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 313, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (332.40, 158.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (237, 2517, 2517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7077) [M::mem_pestat] mean and std.dev: (1556.08, 1165.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9357) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 242, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (218.97, 80.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682396 reads in 165.890 CPU sec, 5.290 real sec [M::mem_process_seqs] Processed 682196 reads in 108.044 CPU sec, 3.435 real sec sorting block 116, #reads = 1073858 mem = 536881290 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 281338, 15, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 223, 540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1212) [M::mem_pestat] mean and std.dev: (387.63, 286.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 313, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (331.54, 156.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (721, 956, 3305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8473) [M::mem_pestat] mean and std.dev: (2140.20, 2402.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11751) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 420, 756) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1890) [M::mem_pestat] mean and std.dev: (423.56, 247.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2457) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682232 sequences (100000209 bp) in 2.734 CPU sec, 2.829 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 281203, 12, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (254, 338, 548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1136) [M::mem_pestat] mean and std.dev: (372.49, 190.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1430) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 312, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (330.37, 156.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (404, 2312, 9560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 27872) [M::mem_pestat] mean and std.dev: (3729.58, 4197.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 37028) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 301, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (284.05, 110.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682368 sequences (100000251 bp) in 1.880 CPU sec, 1.872 real sec... [M::mem_process_seqs] Processed 682088 reads in 167.809 CPU sec, 5.319 real sec [M::mem_process_seqs] Processed 682322 reads in 110.948 CPU sec, 3.511 real sec sorting block 117, #reads = 1072490 mem = 536879332 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 281357, 2, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 283, 929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2421) [M::mem_pestat] mean and std.dev: (556.08, 561.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3167) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 313, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (331.00, 156.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 331, 712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1668) [M::mem_pestat] mean and std.dev: (434.25, 322.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2146) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682506 sequences (100000252 bp) in 2.396 CPU sec, 2.479 real sec... [M::process] read 682642 sequences (100000288 bp) in 1.450 CPU sec, 1.444 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 281154, 12, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 272, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (264.48, 90.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 312, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (330.24, 156.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (562, 1772, 5309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14803) [M::mem_pestat] mean and std.dev: (2756.00, 2527.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19550) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 371, 1340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3568) [M::mem_pestat] mean and std.dev: (799.94, 744.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4682) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682232 reads in 166.834 CPU sec, 5.309 real sec sorting block 118, #reads = 1072404 mem = 536879371 peak = 1144324096 [M::mem_process_seqs] Processed 682368 reads in 109.616 CPU sec, 3.486 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 281593, 7, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 300, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (297.26, 165.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation FR... sorting block 119, #reads = 1072328 mem = 536878313 peak = 1144324096 [M::mem_pestat] (25, 50, 75) percentile: (204, 310, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (329.93, 157.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 335, 575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1273) [M::mem_pestat] mean and std.dev: (379.82, 241.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1622) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682502 sequences (100000069 bp) in 2.541 CPU sec, 2.619 real sec... [M::process] read 682104 sequences (100000219 bp) in 1.440 CPU sec, 1.441 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 281765, 18, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 273, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1243) [M::mem_pestat] mean and std.dev: (308.58, 233.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 309, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (330.30, 158.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (585, 2543, 3517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9381) [M::mem_pestat] mean and std.dev: (2163.22, 1312.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12313) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 376, 689) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1585) [M::mem_pestat] mean and std.dev: (402.20, 271.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2033) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682506 reads in 166.777 CPU sec, 5.306 real sec [M::mem_process_seqs] Processed 682642 reads in 106.596 CPU sec, 3.391 real sec sorting block 120, #reads = 1072885 mem = 536877148 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 281479, 12, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 364, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060) [M::mem_pestat] mean and std.dev: (325.75, 180.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1347) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 310, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (330.11, 158.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (436, 739, 8146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 23566) [M::mem_pestat] mean and std.dev: (3195.17, 3540.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 31276) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 311, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988) [M::mem_pestat] mean and std.dev: (293.20, 137.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1257) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682466 sequences (100000157 bp) in 2.184 CPU sec, 2.263 real sec... [M::process] read 683876 sequences (100000263 bp) in 1.520 CPU sec, 1.523 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281549, 8, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 262, 1402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3796) [M::mem_pestat] mean and std.dev: (687.84, 729.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4993) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 312, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (332.94, 159.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 300, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (326.49, 169.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1075) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682502 reads in 166.841 CPU sec, 5.314 real sec [M::mem_process_seqs] Processed 682104 reads in 108.435 CPU sec, 3.450 real sec sorting block 121, #reads = 1071771 mem = 536879789 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 281308, 10, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 288, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (264.24, 106.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 309, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (330.93, 159.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (301, 907, 2454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6760) [M::mem_pestat] mean and std.dev: (1378.70, 1226.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8913) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 311, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1081) [M::mem_pestat] mean and std.dev: (335.32, 192.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1384) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691182 sequences (100000162 bp) in 2.678 CPU sec, 2.753 real sec... [M::process] read 690310 sequences (100000121 bp) in 1.960 CPU sec, 1.958 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 281150, 7, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 249, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1204) [M::mem_pestat] mean and std.dev: (308.33, 185.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 305, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (327.34, 160.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 272, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (224.79, 76.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682466 reads in 184.459 CPU sec, 5.856 real sec [M::mem_process_seqs] Processed 683876 reads in 127.764 CPU sec, 4.053 real sec sorting block 122, #reads = 1070680 mem = 536884513 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 284584, 6, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 176, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (198.79, 114.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 264, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (290.72, 144.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 246, 667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1595) [M::mem_pestat] mean and std.dev: (386.58, 274.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2059) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688908 sequences (100000198 bp) in 2.411 CPU sec, 2.502 real sec... [M::process] read 687618 sequences (100000242 bp) in 1.920 CPU sec, 1.917 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 284283, 5, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 398, 1368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3746) [M::mem_pestat] mean and std.dev: (666.30, 657.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4935) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 269, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (294.15, 144.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1026) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (244, 416, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (349.14, 144.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691182 reads in 180.549 CPU sec, 5.730 real sec sorting block 123, #reads = 1062931 mem = 536881012 peak = 1144324096 [M::mem_process_seqs] Processed 690310 reads in 116.242 CPU sec, 3.690 real sec sorting block 124, #reads = 1072389 mem = 536879786 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 283765, 6, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 267, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (248.39, 89.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 274, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (298.44, 145.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 1053, 2554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7252) [M::mem_pestat] mean and std.dev: (1369.97, 1155.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9601) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686782 sequences (100000251 bp) in 2.619 CPU sec, 2.664 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 283845, 7, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 302, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1230) [M::mem_pestat] mean and std.dev: (359.93, 244.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 282, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (304.72, 148.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 270, 499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1149) [M::mem_pestat] mean and std.dev: (291.07, 188.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1474) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686016 sequences (100000070 bp) in 1.960 CPU sec, 1.955 real sec... [M::mem_process_seqs] Processed 688908 reads in 172.323 CPU sec, 5.461 real sec [M::mem_process_seqs] Processed 687618 reads in 109.045 CPU sec, 3.447 real sec sorting block 125, #reads = 1070982 mem = 536883013 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 283826, 14, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 363, 1380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3744) [M::mem_pestat] mean and std.dev: (627.60, 597.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4926) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 288, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (309.31, 149.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1077) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (730, 1091, 2736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6748) [M::mem_pestat] mean and std.dev: (1643.85, 1531.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8754) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 340, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952) [M::mem_pestat] mean and std.dev: (297.45, 128.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1210) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685028 sequences (100000041 bp) in 2.881 CPU sec, 2.986 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 283120, 6, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 244, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (245.49, 94.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 292, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (313.00, 150.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1091) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 218, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (224.00, 104.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684780 sequences (100000028 bp) in 1.970 CPU sec, 1.972 real sec... [M::mem_process_seqs] Processed 686782 reads in 167.910 CPU sec, 5.322 real sec [M::mem_process_seqs] Processed 686016 reads in 109.617 CPU sec, 3.460 real sec sorting block 126, #reads = 1072979 mem = 536881793 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 282033, 6, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 280, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (249.00, 95.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 298, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (318.51, 152.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 305, 669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1611) [M::mem_pestat] mean and std.dev: (361.96, 242.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2082) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684112 sequences (100000172 bp) in 2.918 CPU sec, 2.996 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282891, 11, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (281, 315, 608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1262) [M::mem_pestat] mean and std.dev: (411.29, 240.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 300, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (320.19, 153.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (766, 1100, 4226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11146) [M::mem_pestat] mean and std.dev: (1831.64, 1489.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14606) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 274, 586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1444) [M::mem_pestat] mean and std.dev: (377.33, 306.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1873) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683714 sequences (100000120 bp) in 1.530 CPU sec, 1.525 real sec... [M::mem_process_seqs] Processed 685028 reads in 164.498 CPU sec, 5.223 real sec sorting block 127, #reads = 1072669 mem = 536880375 peak = 1144324096 [M::mem_process_seqs] Processed 684780 reads in 108.207 CPU sec, 3.424 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282545, 12, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 238, 819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2179) [M::mem_pestat] mean and std.dev: (381.56, 308.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2859) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 305, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (323.65, 154.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (422, 1534, 5738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16370) [M::mem_pestat] mean and std.dev: (2267.33, 2255.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21686) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 355, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (278.87, 130.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 128, #reads = 1072467 mem = 536880310 peak = 1144324096 [M::process] read 684040 sequences (100000204 bp) in 3.221 CPU sec, 3.306 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 282116, 6, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 265, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1228) [M::mem_pestat] mean and std.dev: (294.42, 194.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 307, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (325.79, 155.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 241, 662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1614) [M::mem_pestat] mean and std.dev: (315.36, 249.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2090) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684112 reads in 173.668 CPU sec, 5.493 real sec [M::process] read 683866 sequences (100000049 bp) in 2.368 CPU sec, 2.377 real sec... [M::mem_process_seqs] Processed 683714 reads in 115.077 CPU sec, 3.623 real sec sorting block 129, #reads = 1072279 mem = 536879589 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 282507, 6, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 172, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (215.74, 120.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 306, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (326.59, 157.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 194, 576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1412) [M::mem_pestat] mean and std.dev: (238.76, 146.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1830) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683416 sequences (100000286 bp) in 3.202 CPU sec, 3.292 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 282415, 1, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 324, 2405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6835) [M::mem_pestat] mean and std.dev: (1443.16, 1733.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9050) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 305, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (326.20, 157.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 285, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014) [M::mem_pestat] mean and std.dev: (287.26, 131.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1295) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683556 sequences (100000130 bp) in 1.860 CPU sec, 1.862 real sec... [M::mem_process_seqs] Processed 684040 reads in 176.253 CPU sec, 5.587 real sec [M::mem_process_seqs] Processed 683866 reads in 117.381 CPU sec, 3.707 real sec sorting block 130, #reads = 1071540 mem = 536879969 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282124, 8, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 266, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (265.64, 94.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 305, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (325.74, 155.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 390, 632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1422) [M::mem_pestat] mean and std.dev: (337.24, 149.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1817) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682944 sequences (100000055 bp) in 2.828 CPU sec, 2.898 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 281752, 18, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 273, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936) [M::mem_pestat] mean and std.dev: (288.87, 134.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1187) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 304, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (325.53, 156.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (450, 775, 1841) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4623) [M::mem_pestat] mean and std.dev: (959.88, 815.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6014) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 336, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1165) [M::mem_pestat] mean and std.dev: (358.14, 177.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1484) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683460 sequences (100000129 bp) in 1.900 CPU sec, 1.895 real sec... [M::mem_process_seqs] Processed 683416 reads in 173.877 CPU sec, 5.512 real sec [M::mem_process_seqs] Processed 683556 reads in 113.417 CPU sec, 3.586 real sec sorting block 131, #reads = 1071831 mem = 536876835 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 281900, 6, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 301, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (281.25, 130.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1021) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 307, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (328.11, 157.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 253, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1085) [M::mem_pestat] mean and std.dev: (316.33, 175.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1386) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683480 sequences (100000282 bp) in 2.725 CPU sec, 2.798 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 281912, 8, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 166, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (190.17, 89.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 305, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (326.81, 157.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1136) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 348, 567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1223) [M::mem_pestat] mean and std.dev: (382.32, 243.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1551) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682804 sequences (100000072 bp) in 1.950 CPU sec, 1.952 real sec... [M::mem_process_seqs] Processed 682944 reads in 172.113 CPU sec, 5.455 real sec sorting block 132, #reads = 1071680 mem = 536879527 peak = 1144324096 [M::mem_process_seqs] Processed 683460 reads in 112.655 CPU sec, 3.567 real sec sorting block 133, #reads = 1072092 mem = 536881324 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 281826, 13, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 232, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1128) [M::mem_pestat] mean and std.dev: (347.64, 253.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 303, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (325.88, 157.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (157, 364, 959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2563) [M::mem_pestat] mean and std.dev: (369.33, 305.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3365) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (302, 433, 1386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3554) [M::mem_pestat] mean and std.dev: (647.74, 537.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4638) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682828 sequences (100000206 bp) in 2.512 CPU sec, 2.610 real sec... [M::process] read 682594 sequences (100000018 bp) in 1.420 CPU sec, 1.422 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 281548, 14, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 219, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (246.36, 121.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 308, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (330.09, 159.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (184, 2283, 2283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6481) [M::mem_pestat] mean and std.dev: (1865.57, 1393.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8580) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 275, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (262.66, 112.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683480 reads in 167.898 CPU sec, 5.341 real sec [M::mem_process_seqs] Processed 682804 reads in 108.966 CPU sec, 3.469 real sec sorting block 134, #reads = 1073651 mem = 536879612 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 281683, 9, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 219, 593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1419) [M::mem_pestat] mean and std.dev: (252.31, 157.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1832) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 308, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (329.45, 158.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 325, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (301.04, 104.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682428 sequences (100000094 bp) in 2.757 CPU sec, 2.818 real sec... [M::process] read 682086 sequences (100000193 bp) in 1.440 CPU sec, 1.438 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 281619, 19, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 268, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (278.72, 118.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 308, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (329.02, 158.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (838, 1679, 2382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5470) [M::mem_pestat] mean and std.dev: (1441.18, 1029.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7014) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 307, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (330.34, 161.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1093) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682828 reads in 162.956 CPU sec, 5.177 real sec [M::mem_process_seqs] Processed 682594 reads in 105.471 CPU sec, 3.346 real sec sorting block 135, #reads = 1074172 mem = 536875782 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 281994, 12, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (240, 310, 546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1158) [M::mem_pestat] mean and std.dev: (338.36, 164.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 310, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (331.62, 160.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (8, 175, 2114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6326) [M::mem_pestat] mean and std.dev: (493.55, 889.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8432) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 277, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (248.62, 99.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681990 sequences (100000194 bp) in 2.928 CPU sec, 2.989 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 281211, 5, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 201, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (241.17, 124.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 311, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (332.37, 160.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 328, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (274.12, 90.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682428 reads in 162.930 CPU sec, 5.162 real sec [M::process] read 681918 sequences (100000142 bp) in 1.840 CPU sec, 1.838 real sec... sorting block 136, #reads = 1074961 mem = 536880092 peak = 1144324096 [M::mem_process_seqs] Processed 682086 reads in 107.661 CPU sec, 3.392 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 281019, 14, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 191, 550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1370) [M::mem_pestat] mean and std.dev: (265.54, 215.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 314, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (334.31, 160.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (305, 1902, 2628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7274) [M::mem_pestat] mean and std.dev: (1587.43, 1325.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9597) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 265, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (223.45, 67.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 137, #reads = 1075655 mem = 536881345 peak = 1144324096 [M::process] read 599014 sequences (87774638 bp) in 2.539 CPU sec, 2.593 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 281419, 11, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (227, 439, 1051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2699) [M::mem_pestat] mean and std.dev: (450.62, 452.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 314, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (335.97, 163.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1158) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (73, 123, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1267) [M::mem_pestat] mean and std.dev: (255.82, 317.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1665) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (252, 418, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029) [M::mem_pestat] mean and std.dev: (361.71, 175.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1288) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681990 reads in 159.773 CPU sec, 5.126 real sec [M::mem_process_seqs] Processed 681918 reads in 103.053 CPU sec, 3.317 real sec sorting block 138, #reads = 1074244 mem = 536877679 peak = 1144324096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 247006, 7, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 251, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (263.86, 109.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 312, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (333.79, 162.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (252, 331, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (308.10, 110.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 599014 reads in 82.438 CPU sec, 2.726 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 149450552 overall: 70060257280 mem 22450.556 user 521.062 sys 760.997 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_4XR-T1-TDNA-1_A23MHWWLT4_1.L003\tPU:L003\tSM:1136_4XR-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_4XR-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1136_4XR-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 1136_4XR-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 761.048 sec; CPU: 22971.634 sec sorting block 139, #reads = 920438 mem = 460682643 peak = 1144324096 uniq header count 1 merging 140 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 8096815861 output reads: 150352062 bam_mem_sort: 140 calls 82.311 user 1.645 sys 84.581 real bam_write: 140 calls 49.492 user 3.312 sys 65.621 real execute: 1 calls 42.422 user 1.466 sys 812.073 real merge_files: 1 calls 42.371 user 1.465 sys 50.286 real parse_chunk: 2238 calls 96.424 user 24.535 sys 127.513 real read_chunk: 6038 calls 47.087 user 42.536 sys 694.884 real sort_block: 1 calls 32.277 user 41.817 sys 672.983 real write_chunk: 3798 calls 42.013 user 1.184 sys 49.375 real overall: 3345489920 mem 1996.048 user 90.578 sys 812.095 real ls: cannot access '*.cram': No such file or directory