This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1029_N5D-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1766.251 CPU sec, 239.549 real sec
[M::process] read 686460 sequences (100000123 bp) in 1.252 CPU sec, 1.259 real sec...
[M::process] read 684322 sequences (100000248 bp) in 1.879 CPU sec, 1.883 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 279512, 6, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 248, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621)
[M::mem_pestat] mean and std.dev: (234.47, 117.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 780)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 316, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (336.90, 167.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 331, 594)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1418)
[M::mem_pestat] mean and std.dev: (389.03, 264.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1830)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683558 sequences (100000179 bp) in 1.910 CPU sec, 1.901 real sec...
[M::process] read 682962 sequences (100000160 bp) in 1.820 CPU sec, 1.827 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 279131, 6, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 254, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (302.55, 177.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 329, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (346.66, 168.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 302, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (290.69, 131.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686460 reads in 233.074 CPU sec, 7.375 real sec
[M::mem_process_seqs] Processed 684322 reads in 142.356 CPU sec, 4.546 real sec
sorting block 0, #reads = 1072521 mem = 536882938 peak = 606924800
[M::process] read 681814 sequences (100000227 bp) in 2.282 CPU sec, 2.331 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 278510, 9, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 244, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (297.16, 137.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1099)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 335, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (351.81, 170.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 272, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (262.24, 119.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681840 sequences (100000022 bp) in 1.920 CPU sec, 1.919 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 278840, 13, 52)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 226, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (244.00, 135.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 339, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (355.80, 172.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1133, 3019, 3019)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6791)
[M::mem_pestat] mean and std.dev: (2313.15, 1073.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8677)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 301, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (300.81, 128.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683558 reads in 204.977 CPU sec, 6.515 real sec
[M::mem_process_seqs] Processed 682962 reads in 231.460 CPU sec, 7.374 real sec
sorting block 1, #reads = 1071099 mem = 536880761 peak = 734326784
[M::process] read 681530 sequences (100000261 bp) in 2.257 CPU sec, 2.331 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278580, 7, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 217, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (205.00, 90.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 345, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (361.27, 173.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 220, 286)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526)
[M::mem_pestat] mean and std.dev: (241.39, 99.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 646)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681606 sequences (100000101 bp) in 1.840 CPU sec, 1.856 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 278833, 8, 54)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 317, 1524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4254)
[M::mem_pestat] mean and std.dev: (657.45, 685.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5619)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 346, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (361.74, 173.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 383, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (342.73, 163.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1292)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681840 reads in 209.655 CPU sec, 6.661 real sec
sorting block 2, #reads = 1070928 mem = 536880724 peak = 852045824
[M::mem_process_seqs] Processed 681814 reads in 236.280 CPU sec, 7.530 real sec
[M::process] read 681956 sequences (100000127 bp) in 2.397 CPU sec, 2.474 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 277487, 14, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 214, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (211.00, 99.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 343, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (359.55, 171.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (960, 2105, 3040)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7200)
[M::mem_pestat] mean and std.dev: (1749.92, 1371.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9280)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 252, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (260.42, 102.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 681884 sequences (100000078 bp) in 2.010 CPU sec, 2.003 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 277429, 7, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 278, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (262.34, 100.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 335, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (351.35, 166.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 270, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (311.97, 169.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 277953, 1, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 299, 601)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1415)
[M::mem_pestat] mean and std.dev: (341.89, 282.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1822)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 339, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (355.05, 168.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 318, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724)
[M::mem_pestat] mean and std.dev: (314.20, 136.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681530 reads in 299.700 CPU sec, 9.610 real sec
sorting block 3, #reads = 1070454 mem = 536878520 peak = 852045824
[M::mem_process_seqs] Processed 681956 reads in 230.942 CPU sec, 7.476 real sec
[M::mem_process_seqs] Processed 681606 reads in 257.660 CPU sec, 8.345 real sec
sorting block 4, #reads = 1068157 mem = 536882940 peak = 896290816
[M::process] read 681614 sequences (100000055 bp) in 2.435 CPU sec, 2.574 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 278042, 6, 59)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 238, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (282.70, 147.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 335, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (351.53, 166.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 299, 1163)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3077)
[M::mem_pestat] mean and std.dev: (611.60, 638.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4034)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681532 sequences (100000244 bp) in 2.237 CPU sec, 2.305 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 277657, 2, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 249, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (264.00, 127.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 338, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (353.87, 167.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 319, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1090)
[M::mem_pestat] mean and std.dev: (330.73, 170.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1382)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685466 sequences (100000117 bp) in 1.880 CPU sec, 1.879 real sec...
[M::mem_process_seqs] Processed 681884 reads in 215.502 CPU sec, 6.833 real sec
sorting block 5, #reads = 1068733 mem = 536876608 peak = 896290816
[M::mem_process_seqs] Processed 681614 reads in 144.986 CPU sec, 4.601 real sec
sorting block 6, #reads = 1067283 mem = 536880941 peak = 939360256
[M::process] read 684034 sequences (100000294 bp) in 2.258 CPU sec, 2.317 real sec...
[M::process] read 683378 sequences (100000099 bp) in 1.540 CPU sec, 1.540 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 278998, 4, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (240, 342, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (290.00, 103.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 333, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (349.72, 170.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 262, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (323.65, 137.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684034 reads in 96.930 CPU sec, 3.096 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 277554, 3, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 199, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (216.70, 117.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 343, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (358.49, 170.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 324, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (331.08, 144.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 279199, 8, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 249, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (271.86, 132.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 324, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (343.64, 170.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 331, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765)
[M::mem_pestat] mean and std.dev: (290.25, 128.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682706 sequences (100000080 bp) in 1.927 CPU sec, 1.930 real sec...
[M::mem_process_seqs] Processed 681532 reads in 273.654 CPU sec, 8.781 real sec
sorting block 7, #reads = 1070526 mem = 536878232 peak = 939360256
[M::mem_process_seqs] Processed 685466 reads in 212.134 CPU sec, 6.762 real sec
[M::process] read 682224 sequences (100000228 bp) in 2.555 CPU sec, 2.621 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 278575, 3, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 329, 895)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2381)
[M::mem_pestat] mean and std.dev: (355.00, 337.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3124)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 338, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (353.71, 170.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 262, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (275.07, 134.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 865)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681870 sequences (100000212 bp) in 2.069 CPU sec, 2.069 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278754, 7, 62)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (211, 265, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (295.18, 128.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 342, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (357.57, 172.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 273, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (282.52, 124.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683378 reads in 198.730 CPU sec, 6.304 real sec
sorting block 8, #reads = 1071866 mem = 536882183 peak = 939360256
[M::mem_process_seqs] Processed 682706 reads in 133.027 CPU sec, 4.232 real sec
sorting block 9, #reads = 1070478 mem = 536889744 peak = 939360256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 278260, 3, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 288, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604)
[M::mem_pestat] mean and std.dev: (254.41, 95.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 346, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (360.53, 173.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 364, 545)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1175)
[M::mem_pestat] mean and std.dev: (384.54, 214.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1490)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681510 sequences (100000272 bp) in 2.676 CPU sec, 2.798 real sec...
[M::process] read 681576 sequences (100000062 bp) in 2.150 CPU sec, 2.151 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 277710, 4, 48)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 271, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (299.89, 154.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 347, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (361.44, 172.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 362, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1136)
[M::mem_pestat] mean and std.dev: (368.76, 241.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1454)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682224 reads in 196.650 CPU sec, 6.225 real sec
[M::mem_process_seqs] Processed 681870 reads in 133.381 CPU sec, 4.257 real sec
sorting block 10, #reads = 1071312 mem = 536879834 peak = 939360256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 278136, 9, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (205, 332, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (317.66, 155.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 344, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (359.33, 170.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 292, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (288.30, 124.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681768 sequences (100000193 bp) in 2.635 CPU sec, 2.698 real sec...
[M::process] read 682154 sequences (100000182 bp) in 2.050 CPU sec, 2.049 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 278317, 7, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 254, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (310.22, 156.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1304)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 340, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (355.57, 167.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 331, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (299.65, 121.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681510 reads in 189.552 CPU sec, 6.023 real sec
sorting block 11, #reads = 1070133 mem = 536880945 peak = 939360256
[M::mem_process_seqs] Processed 681576 reads in 120.090 CPU sec, 3.816 real sec
[M::process] read 681866 sequences (100000077 bp) in 2.221 CPU sec, 2.285 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 277854, 7, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 234, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (272.67, 150.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 337, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (352.63, 166.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 294, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (284.73, 130.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681790 sequences (100000157 bp) in 1.830 CPU sec, 1.837 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 278569, 10, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 238, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (223.55, 95.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 340, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (356.20, 169.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 285, 898)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2630)
[M::mem_pestat] mean and std.dev: (374.70, 406.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3496)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 251, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (277.61, 123.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 897)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 278569, 1, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 217, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586)
[M::mem_pestat] mean and std.dev: (202.10, 78.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 735)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 339, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (354.60, 168.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 288, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (264.34, 104.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 905)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681768 reads in 251.342 CPU sec, 8.007 real sec
sorting block 12, #reads = 1068929 mem = 536878166 peak = 939360256
[M::mem_process_seqs] Processed 681866 reads in 191.623 CPU sec, 6.140 real sec
[M::mem_process_seqs] Processed 682154 reads in 212.937 CPU sec, 6.830 real sec
sorting block 13, #reads = 1068415 mem = 536880807 peak = 939360256
[M::process] read 686086 sequences (100000049 bp) in 2.990 CPU sec, 3.105 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 278633, 6, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (211, 346, 1008)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2602)
[M::mem_pestat] mean and std.dev: (602.43, 620.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3399)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 343, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (358.81, 172.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 290, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (281.55, 100.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 873)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685556 sequences (100000193 bp) in 2.321 CPU sec, 2.328 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 279324, 5, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 339, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (325.24, 164.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 319, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (338.77, 168.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 342, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (327.70, 136.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1105)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681790 reads in 181.738 CPU sec, 5.760 real sec
[M::mem_process_seqs] Processed 686086 reads in 120.978 CPU sec, 3.804 real sec
sorting block 14, #reads = 1068563 mem = 536879651 peak = 939360256
[M::process] read 684476 sequences (100000247 bp) in 2.551 CPU sec, 2.615 real sec...
sorting block 15, #reads = 1067938 mem = 536883330 peak = 939360256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 279360, 7, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 213, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (203.90, 84.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 324, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (342.68, 169.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 305, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (279.89, 112.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683684 sequences (100000214 bp) in 2.562 CPU sec, 2.607 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278890, 4, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 258, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (310.66, 175.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 331, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (348.72, 170.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 343, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1199)
[M::mem_pestat] mean and std.dev: (358.69, 202.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1534)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685556 reads in 174.471 CPU sec, 5.513 real sec
[M::process] read 682930 sequences (100000170 bp) in 2.048 CPU sec, 2.051 real sec...
[M::mem_process_seqs] Processed 684476 reads in 117.073 CPU sec, 3.694 real sec
sorting block 16, #reads = 1072025 mem = 536883182 peak = 939360256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 279492, 3, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 300, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (276.03, 150.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 335, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (352.49, 172.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 296, 598)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1432)
[M::mem_pestat] mean and std.dev: (333.94, 193.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1849)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682672 sequences (100000061 bp) in 2.513 CPU sec, 2.564 real sec...
[M::process] read 682812 sequences (100000001 bp) in 1.590 CPU sec, 1.591 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 279058, 6, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 189, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587)
[M::mem_pestat] mean and std.dev: (207.92, 92.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 735)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 342, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (358.08, 173.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 271, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (281.19, 136.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683684 reads in 175.826 CPU sec, 5.573 real sec
[M::mem_process_seqs] Processed 682930 reads in 121.037 CPU sec, 3.860 real sec
sorting block 17, #reads = 1072553 mem = 536878626 peak = 939360256
[M::process] read 682414 sequences (100000192 bp) in 2.266 CPU sec, 2.330 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 277834, 15, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 259, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (256.71, 112.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1107)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 343, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (358.64, 173.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (419, 562, 1050)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2312)
[M::mem_pestat] mean and std.dev: (635.40, 386.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2943)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 314, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (308.67, 130.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 681514 sequences (100000118 bp) in 1.650 CPU sec, 1.644 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 279041, 3, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (213, 273, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (291.56, 145.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 337, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (353.31, 170.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 246, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1122)
[M::mem_pestat] mean and std.dev: (332.97, 194.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1445)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682672 reads in 174.623 CPU sec, 5.536 real sec
sorting block 18, #reads = 1070342 mem = 536885062 peak = 939360256
[M::mem_process_seqs] Processed 682812 reads in 117.009 CPU sec, 3.762 real sec
[M::process] read 681476 sequences (100000065 bp) in 2.091 CPU sec, 2.166 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 278000, 4, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (281.46, 173.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 337, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (354.82, 170.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 304, 542)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1190)
[M::mem_pestat] mean and std.dev: (316.42, 136.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1514)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681880 sequences (100000029 bp) in 1.630 CPU sec, 1.631 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 278069, 4, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 323, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1071)
[M::mem_pestat] mean and std.dev: (304.65, 145.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1365)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 344, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (359.31, 171.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 237, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (314.50, 158.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1307)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 277903, 4, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 329, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1221)
[M::mem_pestat] mean and std.dev: (433.09, 346.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1817)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 344, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (360.25, 172.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 236, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528)
[M::mem_pestat] mean and std.dev: (231.23, 89.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 640)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682414 reads in 259.594 CPU sec, 8.328 real sec
sorting block 19, #reads = 1070723 mem = 536880239 peak = 939360256
[M::mem_process_seqs] Processed 681514 reads in 196.556 CPU sec, 6.303 real sec
[M::mem_process_seqs] Processed 681476 reads in 219.618 CPU sec, 7.047 real sec
sorting block 20, #reads = 1069108 mem = 536878939 peak = 970645504
[M::process] read 682060 sequences (100000132 bp) in 2.647 CPU sec, 2.761 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 278310, 1, 48)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (213, 355, 549)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1221)
[M::mem_pestat] mean and std.dev: (390.71, 237.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1557)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 342, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (357.90, 171.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 264, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (286.24, 150.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1100)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685384 sequences (100000078 bp) in 1.853 CPU sec, 1.888 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 278377, 10, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 204, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (238.75, 136.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 344, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (361.23, 174.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (229, 881, 881)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2185)
[M::mem_pestat] mean and std.dev: (473.50, 315.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2837)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 264, 578)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1408)
[M::mem_pestat] mean and std.dev: (352.03, 280.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1823)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 685338 sequences (100000194 bp) in 1.590 CPU sec, 1.587 real sec...
[M::mem_process_seqs] Processed 681880 reads in 184.297 CPU sec, 5.839 real sec
sorting block 21, #reads = 1067620 mem = 536881645 peak = 970645504
[M::mem_process_seqs] Processed 682060 reads in 128.768 CPU sec, 4.081 real sec
sorting block 22, #reads = 1067942 mem = 536878359 peak = 970645504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278504, 5, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (196, 248, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1117)
[M::mem_pestat] mean and std.dev: (312.97, 195.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1424)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 322, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (342.14, 169.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 338, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1217)
[M::mem_pestat] mean and std.dev: (338.97, 171.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1559)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684670 sequences (100000135 bp) in 2.558 CPU sec, 2.626 real sec...
[M::process] read 684108 sequences (100000054 bp) in 2.050 CPU sec, 2.053 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 278722, 5, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 293, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (289.03, 151.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 324, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (342.90, 169.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 249, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (263.39, 124.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1086)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685384 reads in 179.638 CPU sec, 5.677 real sec
[M::mem_process_seqs] Processed 685338 reads in 120.100 CPU sec, 3.808 real sec
sorting block 23, #reads = 1074081 mem = 536878593 peak = 970645504
[M::process] read 683372 sequences (100000226 bp) in 2.574 CPU sec, 2.637 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 278471, 5, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (296, 373, 917)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2159)
[M::mem_pestat] mean and std.dev: (582.39, 507.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2780)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 330, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (347.82, 170.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 287, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (322.62, 180.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682730 sequences (100000226 bp) in 2.180 CPU sec, 2.186 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 278716, 5, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 191, 286)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (206.75, 80.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 721)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 332, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (349.30, 170.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 335, 545)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1199)
[M::mem_pestat] mean and std.dev: (338.47, 163.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1526)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684670 reads in 184.199 CPU sec, 5.824 real sec
sorting block 24, #reads = 1071696 mem = 536879381 peak = 970645504
[M::mem_process_seqs] Processed 684108 reads in 212.811 CPU sec, 6.761 real sec
[M::process] read 682592 sequences (100000275 bp) in 2.224 CPU sec, 2.505 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 278185, 5, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 218, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (204.89, 100.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 800)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 338, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (356.01, 173.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 323, 542)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1220)
[M::mem_pestat] mean and std.dev: (306.38, 146.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1559)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681756 sequences (100000299 bp) in 1.618 CPU sec, 1.618 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278699, 6, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 251, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (254.07, 116.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 343, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (359.28, 174.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 473, 1228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3136)
[M::mem_pestat] mean and std.dev: (630.06, 602.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4090)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 278146, 8, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 304, 575)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1355)
[M::mem_pestat] mean and std.dev: (343.80, 191.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1745)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 342, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (358.07, 172.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 305, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (73, 563)
[M::mem_pestat] mean and std.dev: (298.32, 92.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 668)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683372 reads in 245.044 CPU sec, 7.808 real sec
sorting block 25, #reads = 1072009 mem = 536877316 peak = 970645504
[M::mem_process_seqs] Processed 682730 reads in 186.001 CPU sec, 5.956 real sec
[M::mem_process_seqs] Processed 682592 reads in 208.049 CPU sec, 6.668 real sec
sorting block 26, #reads = 1071287 mem = 536885657 peak = 970645504
[M::process] read 681410 sequences (100000284 bp) in 2.725 CPU sec, 2.867 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 277634, 4, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 275, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054)
[M::mem_pestat] mean and std.dev: (319.74, 234.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1352)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 345, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (359.52, 170.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 386, 653)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1483)
[M::mem_pestat] mean and std.dev: (429.06, 301.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1898)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681020 sequences (100000230 bp) in 2.377 CPU sec, 2.397 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 277574, 7, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 274, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (309.36, 162.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 346, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (359.80, 169.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (338, 381, 596)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1112)
[M::mem_pestat] mean and std.dev: (417.70, 208.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1370)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681756 reads in 180.555 CPU sec, 5.710 real sec
[M::mem_process_seqs] Processed 681410 reads in 121.894 CPU sec, 3.829 real sec
sorting block 27, #reads = 1069714 mem = 536880627 peak = 970645504
[M::process] read 681082 sequences (100000298 bp) in 2.077 CPU sec, 2.149 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 277774, 3, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 191, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (211.68, 85.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 347, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (361.37, 170.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 292, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 584)
[M::mem_pestat] mean and std.dev: (295.00, 97.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 694)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683088 sequences (100000130 bp) in 2.167 CPU sec, 2.246 real sec...
[M::process] read 688778 sequences (100000242 bp) in 1.580 CPU sec, 1.584 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 277899, 2, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 325, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (287.70, 149.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1335)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 343, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (357.74, 168.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 362, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1069)
[M::mem_pestat] mean and std.dev: (352.47, 164.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1345)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681020 reads in 180.525 CPU sec, 5.735 real sec
sorting block 28, #reads = 1068519 mem = 536883862 peak = 970645504
[M::mem_process_seqs] Processed 681082 reads in 119.263 CPU sec, 3.803 real sec
sorting block 29, #reads = 1067716 mem = 536879265 peak = 1007005696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 278277, 8, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 214, 602)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1476)
[M::mem_pestat] mean and std.dev: (286.96, 215.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1913)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 334, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (351.44, 169.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 300, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (280.58, 149.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686216 sequences (100000167 bp) in 2.679 CPU sec, 2.735 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 281195, 13, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 243, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (254.71, 101.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 304, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (329.88, 168.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (609, 609, 2097)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5073)
[M::mem_pestat] mean and std.dev: (869.08, 676.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6561)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 261, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687)
[M::mem_pestat] mean and std.dev: (255.71, 104.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 860)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 684446 sequences (100000291 bp) in 2.410 CPU sec, 2.410 real sec...
[M::mem_process_seqs] Processed 683088 reads in 190.496 CPU sec, 6.103 real sec
[M::mem_process_seqs] Processed 688778 reads in 126.660 CPU sec, 4.015 real sec
sorting block 30, #reads = 1066535 mem = 536878883 peak = 1007005696
[M::process] read 683948 sequences (100000013 bp) in 2.103 CPU sec, 2.164 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 280979, 5, 66)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 358, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1189)
[M::mem_pestat] mean and std.dev: (292.04, 146.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1530)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 319, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (340.46, 169.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 293, 554)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1210)
[M::mem_pestat] mean and std.dev: (355.15, 198.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1538)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683236 sequences (100000179 bp) in 1.650 CPU sec, 1.657 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 280211, 4, 51)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 177, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (231.86, 134.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 337, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (354.08, 173.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 324, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (336.98, 147.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 280033, 7, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 290, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (283.65, 141.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 333, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (351.42, 173.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 335, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (312.79, 135.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686216 reads in 259.087 CPU sec, 8.358 real sec
[M::mem_process_seqs] Processed 683948 reads in 194.873 CPU sec, 6.245 real sec
sorting block 31, #reads = 1070083 mem = 536880287 peak = 1007005696
[M::mem_process_seqs] Processed 684446 reads in 215.769 CPU sec, 6.927 real sec
sorting block 32, #reads = 1070163 mem = 536877974 peak = 1007005696
[M::process] read 682978 sequences (100000183 bp) in 2.842 CPU sec, 2.966 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 278692, 5, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 274, 1077)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2869)
[M::mem_pestat] mean and std.dev: (557.71, 689.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3765)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 344, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (360.25, 175.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 225, 627)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1517)
[M::mem_pestat] mean and std.dev: (327.48, 264.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1962)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682398 sequences (100000272 bp) in 2.022 CPU sec, 2.043 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278527, 6, 52)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 294, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (283.90, 119.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 918)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 340, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (356.70, 172.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 211, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (238.92, 116.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 777)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683236 reads in 190.525 CPU sec, 6.037 real sec
[M::process] read 682498 sequences (100000082 bp) in 1.675 CPU sec, 1.674 real sec...
[M::mem_process_seqs] Processed 682978 reads in 136.371 CPU sec, 4.309 real sec
sorting block 33, #reads = 1068054 mem = 536881684 peak = 1007005696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 278682, 4, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 241, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (290.65, 170.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 972)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 340, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (357.39, 172.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 235, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (310.90, 200.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682864 sequences (100000220 bp) in 2.347 CPU sec, 2.433 real sec...
[M::process] read 682522 sequences (100000175 bp) in 1.660 CPU sec, 1.656 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 278467, 8, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 292, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (271.56, 124.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1090)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 339, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (356.03, 171.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 255, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (259.73, 128.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682398 reads in 182.809 CPU sec, 5.802 real sec
sorting block 34, #reads = 1065258 mem = 536881485 peak = 1007005696
[M::mem_process_seqs] Processed 682498 reads in 126.729 CPU sec, 4.037 real sec
[M::process] read 682108 sequences (100000099 bp) in 2.414 CPU sec, 2.467 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 278917, 8, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 288, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056)
[M::mem_pestat] mean and std.dev: (287.96, 158.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1355)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 339, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (356.26, 171.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 211, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (251.00, 138.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 690712 sequences (100000208 bp) in 1.740 CPU sec, 1.742 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 278252, 6, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 245, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (239.37, 108.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 880)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 336, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (353.76, 170.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 286, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (290.64, 105.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 919)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682522 reads in 178.601 CPU sec, 5.655 real sec
sorting block 35, #reads = 1065428 mem = 536878588 peak = 1007005696
[M::mem_process_seqs] Processed 682864 reads in 201.147 CPU sec, 6.385 real sec
sorting block 36, #reads = 1064312 mem = 536879216 peak = 1087119360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 278671, 13, 51)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (226, 290, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (281.52, 88.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1070)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 340, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (357.35, 171.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (360, 1044, 1604)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4092)
[M::mem_pestat] mean and std.dev: (872.58, 578.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5336)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 372, 553)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1383)
[M::mem_pestat] mean and std.dev: (349.30, 197.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1798)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 689184 sequences (100000035 bp) in 2.912 CPU sec, 2.950 real sec...
[M::process] read 687412 sequences (100000163 bp) in 1.740 CPU sec, 1.741 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 281460, 6, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 242, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (242.52, 110.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 294, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (317.27, 159.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 222, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (249.31, 105.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682108 reads in 186.195 CPU sec, 5.887 real sec
[M::mem_process_seqs] Processed 690712 reads in 208.816 CPU sec, 6.626 real sec
sorting block 37, #reads = 1063355 mem = 536879040 peak = 1087119360
[M::process] read 685602 sequences (100000277 bp) in 2.395 CPU sec, 2.430 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 281604, 11, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 244, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (269.45, 119.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 300, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (320.83, 158.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1036, 1278, 2306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4846)
[M::mem_pestat] mean and std.dev: (1455.18, 702.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6116)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 378, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (342.91, 154.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 684910 sequences (100000058 bp) in 1.707 CPU sec, 1.714 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 280713, 3, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (216, 263, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (314.84, 158.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 322, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (338.71, 164.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 231, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (243.63, 121.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 280568, 5, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 272, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (278.89, 128.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 930)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 312, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (330.95, 162.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 259, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (294.28, 166.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1086)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 689184 reads in 264.872 CPU sec, 8.451 real sec
[M::mem_process_seqs] Processed 685602 reads in 200.063 CPU sec, 6.417 real sec
sorting block 38, #reads = 1063285 mem = 536891891 peak = 1087119360
[M::mem_process_seqs] Processed 687412 reads in 223.137 CPU sec, 7.169 real sec
sorting block 39, #reads = 1064325 mem = 536878972 peak = 1087119360
[M::process] read 684326 sequences (100000070 bp) in 2.952 CPU sec, 3.060 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 279784, 4, 55)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 253, 573)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1381)
[M::mem_pestat] mean and std.dev: (369.62, 312.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1785)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 327, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (345.10, 168.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 241, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1134)
[M::mem_pestat] mean and std.dev: (339.49, 226.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1452)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683086 sequences (100000096 bp) in 1.669 CPU sec, 1.678 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 279453, 12, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 274, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (269.09, 86.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 329, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (347.06, 168.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1117, 1584, 9362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 25852)
[M::mem_pestat] mean and std.dev: (3283.33, 3601.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 34097)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 297, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (22, 517)
[M::mem_pestat] mean and std.dev: (280.00, 101.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 685)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684910 reads in 179.195 CPU sec, 5.699 real sec
[M::process] read 682782 sequences (100000104 bp) in 1.636 CPU sec, 1.632 real sec...
sorting block 40, #reads = 1064453 mem = 536879566 peak = 1087119360
[M::mem_process_seqs] Processed 684326 reads in 118.121 CPU sec, 3.945 real sec
[M::process] read 682558 sequences (100000090 bp) in 2.317 CPU sec, 2.385 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 278824, 2, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 321, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (328.00, 163.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 340, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (357.64, 173.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 332, 641)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1431)
[M::mem_pestat] mean and std.dev: (393.50, 213.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1826)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682464 sequences (100000108 bp) in 1.640 CPU sec, 1.642 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 279187, 6, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 256, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (260.55, 104.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 338, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (354.37, 171.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 266, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617)
[M::mem_pestat] mean and std.dev: (260.68, 96.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 756)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682782 reads in 175.687 CPU sec, 5.767 real sec
sorting block 41, #reads = 1065600 mem = 536881161 peak = 1087119360
[M::mem_process_seqs] Processed 683086 reads in 193.895 CPU sec, 6.393 real sec
sorting block 42, #reads = 1065430 mem = 536878624 peak = 1087119360
[M::process] read 685066 sequences (100000289 bp) in 2.172 CPU sec, 2.225 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 278718, 2, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 216, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (236.53, 123.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 884)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 343, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (362.48, 178.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 300, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (342.33, 173.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 688554 sequences (100000121 bp) in 1.700 CPU sec, 1.696 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 279197, 3, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 273, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (299.89, 145.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1061)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 342, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (360.14, 176.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 330, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (326.98, 159.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682464 reads in 174.150 CPU sec, 5.554 real sec
[M::mem_process_seqs] Processed 682558 reads in 190.953 CPU sec, 6.176 real sec
sorting block 43, #reads = 1065414 mem = 536878702 peak = 1087119360
[M::process] read 686230 sequences (100000094 bp) in 2.116 CPU sec, 2.177 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 279166, 8, 53)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 240, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (296.07, 154.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 327, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (349.38, 175.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 295, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (291.81, 114.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 852)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684992 sequences (100000171 bp) in 1.657 CPU sec, 1.660 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 280639, 8, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 222, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (236.06, 97.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 926)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 318, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (340.72, 171.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 323, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1124)
[M::mem_pestat] mean and std.dev: (302.83, 136.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1430)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 281249, 1, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 260, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (279.17, 152.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1084)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 305, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (329.07, 167.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 261, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1203)
[M::mem_pestat] mean and std.dev: (288.13, 159.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1545)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685066 reads in 253.392 CPU sec, 8.165 real sec
[M::mem_process_seqs] Processed 686230 reads in 187.334 CPU sec, 6.009 real sec
sorting block 44, #reads = 1062216 mem = 536877993 peak = 1087119360
[M::mem_process_seqs] Processed 688554 reads in 203.474 CPU sec, 6.533 real sec
sorting block 45, #reads = 1071243 mem = 536876206 peak = 1087119360
[M::process] read 684168 sequences (100000204 bp) in 2.322 CPU sec, 2.427 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 279879, 4, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 241, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (273.90, 123.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 326, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (345.11, 170.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 295, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (296.32, 139.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 955)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684348 sequences (100000142 bp) in 1.802 CPU sec, 1.816 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 279716, 1, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 307, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (285.35, 156.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 330, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (347.97, 169.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 240, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (262.67, 164.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684992 reads in 164.888 CPU sec, 5.227 real sec
[M::process] read 684654 sequences (100000162 bp) in 1.662 CPU sec, 1.671 real sec...
[M::mem_process_seqs] Processed 684168 reads in 109.087 CPU sec, 3.439 real sec
sorting block 46, #reads = 1070151 mem = 536879931 peak = 1087119360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 279035, 6, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 269, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1132)
[M::mem_pestat] mean and std.dev: (298.33, 162.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1460)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 328, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (345.53, 167.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 318, 538)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1186)
[M::mem_pestat] mean and std.dev: (336.38, 153.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1510)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685088 sequences (100000004 bp) in 2.122 CPU sec, 2.181 real sec...
[M::process] read 683782 sequences (100000204 bp) in 1.610 CPU sec, 1.612 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 278987, 5, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 281, 882)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2362)
[M::mem_pestat] mean and std.dev: (414.48, 369.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3102)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 325, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (344.80, 168.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 260, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (267.84, 105.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 691)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684348 reads in 170.612 CPU sec, 5.428 real sec
sorting block 47, #reads = 1069452 mem = 536885317 peak = 1087119360
[M::mem_process_seqs] Processed 684654 reads in 111.666 CPU sec, 3.653 real sec
[M::process] read 683232 sequences (100000179 bp) in 1.978 CPU sec, 2.027 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 279944, 5, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (118, 208, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (205.48, 97.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 322, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (342.17, 168.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 256, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (286.94, 123.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683226 sequences (100000131 bp) in 1.650 CPU sec, 1.647 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 279097, 6, 55)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 244, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (261.25, 131.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 337, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (356.10, 174.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 292, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (298.96, 168.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 279770, 6, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 268, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582)
[M::mem_pestat] mean and std.dev: (248.38, 106.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 718)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 335, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (354.54, 174.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 300, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (328.18, 169.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685088 reads in 244.076 CPU sec, 7.879 real sec
sorting block 48, #reads = 1067945 mem = 536878776 peak = 1087119360
[M::mem_process_seqs] Processed 683232 reads in 188.695 CPU sec, 6.050 real sec
sorting block 49, #reads = 1066537 mem = 536876716 peak = 1087119360
[M::process] read 685986 sequences (100000183 bp) in 2.099 CPU sec, 2.173 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 279094, 5, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 287, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (296.40, 123.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 336, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (356.74, 176.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 368, 567)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1351)
[M::mem_pestat] mean and std.dev: (368.38, 218.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1743)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683226 reads in 98.012 CPU sec, 3.078 real sec
[M::process] read 691686 sequences (100000295 bp) in 1.827 CPU sec, 1.837 real sec...
[M::mem_process_seqs] Processed 683782 reads in 332.167 CPU sec, 10.584 real sec
sorting block 50, #reads = 1065932 mem = 536878136 peak = 1087119360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 279907, 8, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (224, 296, 584)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1304)
[M::mem_pestat] mean and std.dev: (372.82, 230.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1664)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 322, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (345.31, 174.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 299, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (346.56, 182.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1322)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 690678 sequences (100000193 bp) in 1.972 CPU sec, 2.207 real sec...
[M::process] read 687818 sequences (100000011 bp) in 1.660 CPU sec, 1.659 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 281762, 5, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (197, 315, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1112)
[M::mem_pestat] mean and std.dev: (379.67, 272.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1469)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 289, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (311.82, 156.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869)
[M::mem_pestat] mean and std.dev: (264.56, 122.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1103)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685986 reads in 211.453 CPU sec, 6.688 real sec
sorting block 51, #reads = 1062224 mem = 536880572 peak = 1087119360
[M::mem_process_seqs] Processed 691686 reads in 125.727 CPU sec, 3.999 real sec
[M::process] read 686296 sequences (100000234 bp) in 2.082 CPU sec, 2.143 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 281873, 3, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 272, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (332.38, 224.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1331)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 294, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (314.93, 156.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 236, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879)
[M::mem_pestat] mean and std.dev: (271.23, 132.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1111)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685696 sequences (100000243 bp) in 1.660 CPU sec, 1.658 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 280636, 5, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 240, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (242.37, 87.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 782)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 308, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (327.33, 160.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 325, 604)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1432)
[M::mem_pestat] mean and std.dev: (352.65, 211.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1846)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 280614, 6, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (233, 308, 531)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1127)
[M::mem_pestat] mean and std.dev: (310.48, 140.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1425)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 319, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (337.36, 165.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 293, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (288.12, 115.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 873)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 690678 reads in 265.230 CPU sec, 8.479 real sec
sorting block 52, #reads = 1064138 mem = 536879097 peak = 1087119360
[M::mem_process_seqs] Processed 687818 reads in 199.843 CPU sec, 6.420 real sec
[M::mem_process_seqs] Processed 686296 reads in 217.937 CPU sec, 7.006 real sec
sorting block 53, #reads = 1063975 mem = 536879971 peak = 1087119360
[M::process] read 684952 sequences (100000060 bp) in 2.409 CPU sec, 2.521 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 279615, 6, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (121, 222, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (253.15, 149.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 322, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (341.48, 167.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 472, 744)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1820)
[M::mem_pestat] mean and std.dev: (417.40, 233.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2358)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683838 sequences (100000102 bp) in 1.840 CPU sec, 1.858 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 279375, 4, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 244, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771)
[M::mem_pestat] mean and std.dev: (283.54, 152.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 965)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 328, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (346.53, 169.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 356, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (338.41, 146.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683220 sequences (100000184 bp) in 1.750 CPU sec, 1.758 real sec...
[M::mem_process_seqs] Processed 685696 reads in 185.268 CPU sec, 5.860 real sec
sorting block 54, #reads = 1067650 mem = 536881031 peak = 1087119360
[M::mem_process_seqs] Processed 684952 reads in 119.729 CPU sec, 3.801 real sec
sorting block 55, #reads = 1066375 mem = 536878901 peak = 1087119360
[M::process] read 682672 sequences (100000135 bp) in 2.232 CPU sec, 2.318 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 279039, 6, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 282, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (261.12, 102.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 868)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 332, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (348.52, 168.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 319, 552)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1230)
[M::mem_pestat] mean and std.dev: (319.71, 191.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1569)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682100 sequences (100000297 bp) in 1.637 CPU sec, 1.643 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 278547, 8, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 178, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869)
[M::mem_pestat] mean and std.dev: (236.16, 153.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 340, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (355.71, 170.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 219, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (223.33, 109.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 278668, 10, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (235, 342, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (317.48, 125.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1107)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 341, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (356.15, 171.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (616, 1086, 1292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2644)
[M::mem_pestat] mean and std.dev: (801.75, 371.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3320)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 236, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702)
[M::mem_pestat] mean and std.dev: (254.90, 134.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 880)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683838 reads in 255.073 CPU sec, 8.163 real sec
[M::mem_process_seqs] Processed 683220 reads in 190.982 CPU sec, 6.123 real sec
[M::process] read 63218 sequences (9239765 bp) in 0.440 CPU sec, 0.452 real sec...
[M::mem_process_seqs] Processed 682672 reads in 210.806 CPU sec, 6.790 real sec
sorting block 56, #reads = 1066953 mem = 536883398 peak = 1087119360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 278931, 5, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 280, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (287.47, 140.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 342, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (358.04, 171.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 281, 563)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1253)
[M::mem_pestat] mean and std.dev: (364.68, 261.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1598)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 25841, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 329, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (345.06, 166.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 63218 reads in 28.578 CPU sec, 0.929 real sec
[M::mem_process_seqs] Processed 682100 reads in 105.728 CPU sec, 3.576 real sec
sorting block 57, #reads = 1065498 mem = 536877655 peak = 1139216384
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: 1f8bfbff fffab20b
  extended: f1bf07ab 1a407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 62292058
overall: 70099841024 mem 10780.162 user 325.563 sys 544.689 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1029_N5D-T1-TDNA-1_A23MHWWLT4_1.L006\tPU:L006\tSM:1029_N5D-T1-TDNA-1_A23MHWWLT4_1\tLB:1029_N5D-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0001.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0001.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 544.720 sec; CPU: 11105.728 sec
sorting block 58, #reads = 703801 mem = 353955010 peak = 1154482176
uniq header count 1
merging 59 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: 1f8bfbff fffab20b
  extended: f1bf07ab 1a407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8559C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3939413276
output reads: 62658455
bam_mem_sort: 59 calls 30.773 user 0.340 sys 31.169 real
bam_write: 59 calls 14.125 user 0.694 sys 14.862 real
execute: 1 calls 15.483 user 0.780 sys 571.828 real
merge_files: 1 calls 15.453 user 0.749 sys 26.705 real
parse_chunk: 939 calls 53.087 user 11.865 sys 65.790 real
read_chunk: 2537 calls 22.852 user 16.367 sys 306.833 real
sort_block: 1 calls 16.653 user 16.023 sys 296.900 real
write_chunk: 1596 calls 15.090 user 0.512 sys 25.852 real
overall: 2871013376 mem 1028.011 user 37.437 sys 571.846 real
ls: cannot access '*.cram': No such file or directory