cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1047_JG-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1497.452 CPU sec, 52.997 real sec
[M::process] read 704192 sequences (100000267 bp) in 0.985 CPU sec, 0.989 real sec...
[M::process] read 701796 sequences (100000035 bp) in 1.428 CPU sec, 1.430 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292498, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 212, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610)
[M::mem_pestat] mean and std.dev: (235.12, 111.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701124 sequences (100000171 bp) in 1.260 CPU sec, 1.298 real sec...
[M::process] read 700744 sequences (100000195 bp) in 1.243 CPU sec, 1.298 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292019, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.59, 115.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 704192 reads in 154.691 CPU sec, 4.957 real sec
[M::mem_process_seqs] Processed 701796 reads in 85.157 CPU sec, 2.750 real sec
sorting block 0, #reads = 1094688 mem = 536878589 peak = 609972224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291906, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (243.99, 116.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700042 sequences (100000134 bp) in 1.660 CPU sec, 1.726 real sec...
[M::process] read 699174 sequences (100000113 bp) in 1.300 CPU sec, 1.303 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292065, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (245.74, 118.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701124 reads in 126.986 CPU sec, 4.062 real sec
[M::mem_process_seqs] Processed 700744 reads in 81.239 CPU sec, 2.625 real sec
sorting block 1, #reads = 1094757 mem = 536881171 peak = 735801344
[M::process] read 699162 sequences (100000099 bp) in 1.613 CPU sec, 1.691 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291376, 6, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 219, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492)
[M::mem_pestat] mean and std.dev: (208.00, 70.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 601)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (248.63, 120.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698300 sequences (100000024 bp) in 1.308 CPU sec, 1.324 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290731, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.47, 119.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 291568, 1, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (122, 147, 2594)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7538)
[M::mem_pestat] mean and std.dev: (777.19, 1054.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10010)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (251.14, 121.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 236, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 532)
[M::mem_pestat] mean and std.dev: (216.89, 59.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 636)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700042 reads in 186.945 CPU sec, 6.038 real sec
[M::mem_process_seqs] Processed 699174 reads in 139.704 CPU sec, 4.539 real sec
sorting block 2, #reads = 1094238 mem = 536877085 peak = 884416512
[M::mem_process_seqs] Processed 699162 reads in 151.422 CPU sec, 4.936 real sec
sorting block 3, #reads = 1094018 mem = 536885658 peak = 884416512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290622, 10, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.66, 122.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 555, 760)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1990)
[M::mem_pestat] mean and std.dev: (541.10, 384.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2605)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 698136 sequences (100000121 bp) in 2.076 CPU sec, 2.268 real sec...
[M::mem_process_seqs] Processed 698300 reads in 74.169 CPU sec, 2.476 real sec
sorting block 4, #reads = 1094510 mem = 536881723 peak = 884416512
[M::process] read 697844 sequences (100000235 bp) in 1.832 CPU sec, 1.987 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290948, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.80, 121.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696916 sequences (100000277 bp) in 1.283 CPU sec, 1.384 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290961, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.92, 121.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696770 sequences (100000232 bp) in 1.289 CPU sec, 1.281 real sec...
[M::mem_process_seqs] Processed 698136 reads in 137.226 CPU sec, 4.365 real sec
[M::mem_process_seqs] Processed 697844 reads in 88.166 CPU sec, 2.803 real sec
sorting block 5, #reads = 1093362 mem = 536878456 peak = 884416512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 289924, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.47, 119.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697136 sequences (100000164 bp) in 2.138 CPU sec, 2.305 real sec...
[M::process] read 696986 sequences (100000284 bp) in 1.370 CPU sec, 1.375 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290788, 6, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 234, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (223.75, 82.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1083)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.11, 118.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696916 reads in 137.366 CPU sec, 4.394 real sec
[M::mem_process_seqs] Processed 696770 reads in 86.209 CPU sec, 2.749 real sec
sorting block 6, #reads = 1092696 mem = 536877149 peak = 884416512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290365, 3, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (125, 163, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536)
[M::mem_pestat] mean and std.dev: (190.22, 94.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 673)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (250.14, 117.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 795, 2754)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7976)
[M::mem_pestat] mean and std.dev: (1398.60, 1348.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10587)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 697108 sequences (100000183 bp) in 1.752 CPU sec, 1.851 real sec...
[M::process] read 696916 sequences (100000229 bp) in 1.370 CPU sec, 1.366 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290314, 4, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 262, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (277.00, 161.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (250.84, 118.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697136 reads in 136.150 CPU sec, 4.355 real sec
sorting block 7, #reads = 1092690 mem = 536878485 peak = 884416512
[M::mem_process_seqs] Processed 696986 reads in 84.126 CPU sec, 2.681 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290280, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (250.59, 117.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696384 sequences (100000192 bp) in 1.994 CPU sec, 2.130 real sec...
[M::process] read 699776 sequences (100000067 bp) in 1.420 CPU sec, 1.415 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290573, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (252.05, 118.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697108 reads in 141.285 CPU sec, 4.516 real sec
sorting block 8, #reads = 1091705 mem = 536875934 peak = 884416512
[M::mem_process_seqs] Processed 696916 reads in 89.014 CPU sec, 2.845 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 289914, 4, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 234, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (275.90, 129.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (255.05, 120.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 316, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (324.29, 130.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 9, #reads = 1090964 mem = 536876810 peak = 884416512
[M::process] read 702350 sequences (100000049 bp) in 2.053 CPU sec, 2.182 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291343, 7, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (120, 264, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (206.60, 80.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 764)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (246.67, 117.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696384 reads in 130.794 CPU sec, 4.167 real sec
[M::process] read 701748 sequences (100000061 bp) in 1.407 CPU sec, 1.413 real sec...
[M::mem_process_seqs] Processed 699776 reads in 83.241 CPU sec, 2.631 real sec
sorting block 10, #reads = 1088664 mem = 536878431 peak = 884416512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292290, 1, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (241.03, 115.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 381, 742)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1718)
[M::mem_pestat] mean and std.dev: (401.45, 229.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2206)
[M::mem_pestat] skip orientation RR
[M::process] read 701238 sequences (100000296 bp) in 2.172 CPU sec, 2.276 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292320, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (243.50, 116.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 326, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (279.00, 107.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 702350 reads in 124.998 CPU sec, 3.963 real sec
[M::process] read 700270 sequences (100000039 bp) in 1.410 CPU sec, 1.411 real sec...
[M::mem_process_seqs] Processed 701748 reads in 79.217 CPU sec, 2.499 real sec
sorting block 11, #reads = 1095607 mem = 536876628 peak = 884416512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292064, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (245.51, 118.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699690 sequences (100000127 bp) in 1.907 CPU sec, 2.022 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 291210, 5, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (215, 247, 259)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 347)
[M::mem_pestat] mean and std.dev: (230.33, 49.00)
[M::mem_pestat] low and high boundaries for proper pairs: (34, 426)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (247.56, 119.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (307, 394, 1271)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3199)
[M::mem_pestat] mean and std.dev: (379.50, 317.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4163)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 701238 reads in 125.457 CPU sec, 3.995 real sec
[M::process] read 699146 sequences (100000276 bp) in 1.514 CPU sec, 1.523 real sec...
sorting block 12, #reads = 1096835 mem = 536878057 peak = 884416512
[M::mem_process_seqs] Processed 700270 reads in 80.283 CPU sec, 2.532 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291161, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (249.16, 120.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 13, #reads = 1094866 mem = 536878272 peak = 884416512
[M::process] read 699070 sequences (100000192 bp) in 2.231 CPU sec, 2.365 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291448, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (251.19, 121.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699690 reads in 125.121 CPU sec, 3.978 real sec
[M::mem_process_seqs] Processed 699146 reads in 79.644 CPU sec, 2.512 real sec
[M::process] read 698420 sequences (100000116 bp) in 1.551 CPU sec, 1.586 real sec...
sorting block 14, #reads = 1095070 mem = 536879998 peak = 884416512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291211, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 227, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (252.03, 122.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697490 sequences (100000083 bp) in 1.984 CPU sec, 2.129 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291444, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (253.31, 122.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 317, 1943)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5509)
[M::mem_pestat] mean and std.dev: (703.44, 751.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7292)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699070 reads in 124.573 CPU sec, 3.978 real sec
[M::process] read 697294 sequences (100000181 bp) in 1.627 CPU sec, 1.683 real sec...
sorting block 15, #reads = 1094905 mem = 536880134 peak = 884416512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 290553, 0, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 305, 9875)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 29347)
[M::mem_pestat] mean and std.dev: (2712.24, 4022.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 39083)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (254.55, 122.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 696896 sequences (100000178 bp) in 1.612 CPU sec, 1.622 real sec...
[M::mem_process_seqs] Processed 697490 reads in 73.405 CPU sec, 2.327 real sec
[M::mem_process_seqs] Processed 698420 reads in 177.364 CPU sec, 5.627 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290781, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.08, 120.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 16, #reads = 1093991 mem = 536877532 peak = 884416512
[M::process] read 696938 sequences (100000148 bp) in 1.924 CPU sec, 2.040 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 290407, 9, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 172, 896)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2376)
[M::mem_pestat] mean and std.dev: (273.09, 219.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3116)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.49, 119.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697294 reads in 137.369 CPU sec, 4.361 real sec
[M::mem_process_seqs] Processed 696896 reads in 81.164 CPU sec, 2.563 real sec
[M::process] read 696938 sequences (100000041 bp) in 1.710 CPU sec, 1.793 real sec...
sorting block 17, #reads = 1094729 mem = 536879051 peak = 884416512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 290385, 5, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 351, 1186)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3178)
[M::mem_pestat] mean and std.dev: (1005.07, 1047.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5194)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (252.03, 118.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697070 sequences (100000131 bp) in 2.136 CPU sec, 2.277 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290541, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (250.98, 117.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696938 reads in 127.713 CPU sec, 4.071 real sec
[M::mem_process_seqs] Processed 696938 reads in 80.979 CPU sec, 2.554 real sec
[M::process] read 697304 sequences (100000203 bp) in 1.600 CPU sec, 1.632 real sec...
sorting block 18, #reads = 1091538 mem = 536876975 peak = 884416512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290031, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (251.85, 119.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 380, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (340.33, 160.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RR
[M::process] read 697442 sequences (100000240 bp) in 1.985 CPU sec, 2.133 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290502, 8, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (250, 317, 3840)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11020)
[M::mem_pestat] mean and std.dev: (1152.67, 1553.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14610)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (251.41, 118.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697070 reads in 127.731 CPU sec, 4.068 real sec
sorting block 19, #reads = 1091756 mem = 536878057 peak = 884416512
[M::mem_process_seqs] Processed 697304 reads in 81.438 CPU sec, 2.573 real sec
[M::process] read 697254 sequences (100000122 bp) in 1.587 CPU sec, 1.620 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290672, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (252.38, 120.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 20, #reads = 1091400 mem = 536880732 peak = 935809024
[M::process] read 703398 sequences (100000040 bp) in 2.227 CPU sec, 2.362 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 290569, 2, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 256, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (219.69, 69.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (253.83, 121.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697442 reads in 130.488 CPU sec, 4.149 real sec
[M::process] read 703306 sequences (100000028 bp) in 1.457 CPU sec, 1.467 real sec...
[M::mem_process_seqs] Processed 697254 reads in 84.169 CPU sec, 2.651 real sec
sorting block 21, #reads = 1092082 mem = 536878935 peak = 935809024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292572, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (237.77, 113.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703004 sequences (100000224 bp) in 1.999 CPU sec, 2.135 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292427, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626)
[M::mem_pestat] mean and std.dev: (239.19, 114.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 784)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703398 reads in 126.644 CPU sec, 4.037 real sec
[M::mem_process_seqs] Processed 703306 reads in 81.174 CPU sec, 2.558 real sec
[M::process] read 702156 sequences (100000251 bp) in 1.549 CPU sec, 1.581 real sec...
sorting block 22, #reads = 1092762 mem = 536880759 peak = 935809024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292541, 6, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 374, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (316.89, 137.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (240.51, 115.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 701302 sequences (100000250 bp) in 2.147 CPU sec, 2.253 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292439, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.21, 116.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703004 reads in 126.364 CPU sec, 4.024 real sec
[M::process] read 701054 sequences (100000226 bp) in 1.451 CPU sec, 1.456 real sec...
[M::mem_process_seqs] Processed 702156 reads in 80.808 CPU sec, 2.551 real sec
sorting block 23, #reads = 1096850 mem = 536880179 peak = 935809024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292552, 7, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (245.12, 118.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 24, #reads = 1095981 mem = 536880168 peak = 935809024
[M::process] read 700270 sequences (100000119 bp) in 2.023 CPU sec, 2.171 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292127, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 221, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (246.59, 119.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701302 reads in 126.126 CPU sec, 4.016 real sec
[M::process] read 699626 sequences (100000052 bp) in 1.556 CPU sec, 1.580 real sec...
[M::mem_process_seqs] Processed 701054 reads in 80.349 CPU sec, 2.534 real sec
sorting block 25, #reads = 1096135 mem = 536879770 peak = 935809024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291964, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (248.50, 120.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699424 sequences (100000212 bp) in 2.136 CPU sec, 2.256 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 291566, 6, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 323, 906)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2310)
[M::mem_pestat] mean and std.dev: (444.08, 318.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3012)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (250.17, 121.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 700270 reads in 126.933 CPU sec, 4.036 real sec
[M::process] read 698582 sequences (100000253 bp) in 1.454 CPU sec, 1.452 real sec...
[M::mem_process_seqs] Processed 699626 reads in 80.849 CPU sec, 2.548 real sec
sorting block 26, #reads = 1095789 mem = 536882285 peak = 935809024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291686, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.66, 121.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698972 sequences (100000066 bp) in 1.960 CPU sec, 2.078 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291355, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.50, 121.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699424 reads in 125.568 CPU sec, 3.995 real sec
[M::process] read 698504 sequences (100000147 bp) in 1.469 CPU sec, 1.487 real sec...
[M::mem_process_seqs] Processed 698582 reads in 79.941 CPU sec, 2.524 real sec
sorting block 27, #reads = 1095021 mem = 536878061 peak = 935809024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291175, 5, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (222, 238, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (272.00, 113.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (246.43, 116.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697456 sequences (100000158 bp) in 2.192 CPU sec, 2.290 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290345, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.27, 119.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698972 reads in 128.213 CPU sec, 4.072 real sec
[M::process] read 697544 sequences (100000008 bp) in 1.410 CPU sec, 1.413 real sec...
sorting block 28, #reads = 1094248 mem = 536880188 peak = 935809024
[M::mem_process_seqs] Processed 698504 reads in 81.167 CPU sec, 2.560 real sec
sorting block 29, #reads = 1093378 mem = 536878936 peak = 992407552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290681, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (253.24, 121.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697438 sequences (100000043 bp) in 1.959 CPU sec, 2.039 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290712, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (253.03, 121.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697766 sequences (100000186 bp) in 1.400 CPU sec, 1.409 real sec...
[M::mem_process_seqs] Processed 697456 reads in 127.820 CPU sec, 4.061 real sec
[M::mem_process_seqs] Processed 697544 reads in 81.012 CPU sec, 2.558 real sec
sorting block 30, #reads = 1092705 mem = 536879297 peak = 992407552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290535, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.60, 120.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696990 sequences (100000153 bp) in 2.237 CPU sec, 2.351 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290834, 8, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.23, 120.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702296 sequences (100000068 bp) in 1.390 CPU sec, 1.390 real sec...
[M::mem_process_seqs] Processed 697438 reads in 129.562 CPU sec, 4.109 real sec
[M::mem_process_seqs] Processed 697766 reads in 82.569 CPU sec, 2.609 real sec
sorting block 31, #reads = 1092203 mem = 536877298 peak = 992407552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290457, 8, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (254.76, 122.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 343, 895)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2395)
[M::mem_pestat] mean and std.dev: (444.33, 329.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3145)
[M::mem_pestat] skip orientation RR
[M::process] read 703120 sequences (100000152 bp) in 2.031 CPU sec, 2.180 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 292031, 12, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 341, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (257.73, 115.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.63, 116.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (86, 710, 1678)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4862)
[M::mem_pestat] mean and std.dev: (800.36, 796.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6454)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 696990 reads in 128.738 CPU sec, 4.092 real sec
[M::process] read 702728 sequences (100000077 bp) in 1.410 CPU sec, 1.404 real sec...
sorting block 32, #reads = 1091486 mem = 536881970 peak = 992407552
[M::mem_process_seqs] Processed 702296 reads in 81.699 CPU sec, 2.584 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 292635, 2, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (327, 455, 1407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3567)
[M::mem_pestat] mean and std.dev: (799.22, 721.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4647)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626)
[M::mem_pestat] mean and std.dev: (238.94, 114.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 784)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 33, #reads = 1093362 mem = 536877371 peak = 992407552
[M::process] read 702672 sequences (100000196 bp) in 2.283 CPU sec, 2.383 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292869, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (240.03, 114.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 189, 9202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 27260)
[M::mem_pestat] mean and std.dev: (2677.91, 3997.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 36289)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 703120 reads in 124.043 CPU sec, 3.936 real sec
[M::process] read 701696 sequences (100000064 bp) in 1.468 CPU sec, 1.482 real sec...
[M::mem_process_seqs] Processed 702728 reads in 78.636 CPU sec, 2.484 real sec
sorting block 34, #reads = 1097096 mem = 536877753 peak = 992407552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291930, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (240.86, 115.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701456 sequences (100000085 bp) in 2.005 CPU sec, 2.136 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292338, 5, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (242.73, 117.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 178, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1296)
[M::mem_pestat] mean and std.dev: (301.17, 235.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1678)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 702672 reads in 124.833 CPU sec, 3.977 real sec
[M::process] read 700818 sequences (100000028 bp) in 1.517 CPU sec, 1.539 real sec...
[M::mem_process_seqs] Processed 701696 reads in 79.737 CPU sec, 2.509 real sec
sorting block 35, #reads = 1097569 mem = 536882123 peak = 992407552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292054, 3, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (244.28, 117.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 291, 1282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3310)
[M::mem_pestat] mean and std.dev: (587.69, 612.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4324)
[M::mem_pestat] skip orientation RR
[M::process] read 700130 sequences (100000155 bp) in 2.312 CPU sec, 2.468 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291689, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (245.88, 118.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701456 reads in 124.736 CPU sec, 3.968 real sec
[M::mem_process_seqs] Processed 700818 reads in 79.812 CPU sec, 2.513 real sec
[M::process] read 700064 sequences (100000277 bp) in 1.622 CPU sec, 1.660 real sec...
sorting block 36, #reads = 1095045 mem = 536879866 peak = 992407552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291442, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (248.16, 120.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699288 sequences (100000225 bp) in 1.877 CPU sec, 1.983 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292065, 1, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (228, 267, 1173)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3063)
[M::mem_pestat] mean and std.dev: (553.50, 482.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4008)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (249.65, 121.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 700130 reads in 124.594 CPU sec, 3.968 real sec
sorting block 37, #reads = 1095717 mem = 536877865 peak = 992407552
[M::process] read 698568 sequences (100000013 bp) in 1.501 CPU sec, 1.518 real sec...
[M::mem_process_seqs] Processed 700064 reads in 79.558 CPU sec, 2.505 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291299, 4, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (219, 249, 547)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1203)
[M::mem_pestat] mean and std.dev: (296.33, 150.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1531)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (250.33, 121.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (323, 465, 854)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1916)
[M::mem_pestat] mean and std.dev: (439.90, 243.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2447)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 38, #reads = 1095863 mem = 536876321 peak = 1001828352
[M::process] read 698248 sequences (100000199 bp) in 2.062 CPU sec, 2.210 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291287, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.20, 121.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699288 reads in 125.840 CPU sec, 4.013 real sec
[M::mem_process_seqs] Processed 698568 reads in 79.903 CPU sec, 2.517 real sec
[M::process] read 697368 sequences (100000299 bp) in 1.656 CPU sec, 1.706 real sec...
sorting block 39, #reads = 1093594 mem = 536879250 peak = 1001828352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291008, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.68, 121.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696710 sequences (100000255 bp) in 1.807 CPU sec, 1.905 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290637, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (255.19, 122.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 852)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698248 reads in 126.604 CPU sec, 4.036 real sec
[M::process] read 696230 sequences (100000235 bp) in 1.489 CPU sec, 1.515 real sec...
[M::mem_process_seqs] Processed 697368 reads in 80.940 CPU sec, 2.546 real sec
sorting block 40, #reads = 1093575 mem = 536879276 peak = 1001828352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291206, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (256.05, 122.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 853)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696220 sequences (100000101 bp) in 2.205 CPU sec, 2.320 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290062, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (255.59, 121.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 849)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696710 reads in 125.290 CPU sec, 3.983 real sec
[M::mem_process_seqs] Processed 696230 reads in 80.358 CPU sec, 2.529 real sec
[M::process] read 696434 sequences (100000085 bp) in 1.583 CPU sec, 1.610 real sec...
sorting block 41, #reads = 1092393 mem = 536878623 peak = 1001828352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 289889, 10, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (254.24, 120.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (260, 661, 992)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2456)
[M::mem_pestat] mean and std.dev: (616.90, 398.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3188)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 42, #reads = 1092092 mem = 536880137 peak = 1001828352
[M::process] read 700166 sequences (100000249 bp) in 1.890 CPU sec, 1.997 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 289806, 3, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (219, 335, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (302.90, 187.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.72, 120.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696220 reads in 127.645 CPU sec, 4.067 real sec
[M::process] read 706796 sequences (100000000 bp) in 1.563 CPU sec, 1.582 real sec...
[M::mem_process_seqs] Processed 696434 reads in 81.957 CPU sec, 2.583 real sec
sorting block 43, #reads = 1090223 mem = 536879342 peak = 1001828352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291317, 5, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (244.41, 116.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 800)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (401, 489, 1636)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4106)
[M::mem_pestat] mean and std.dev: (780.80, 576.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5341)
[M::mem_pestat] skip orientation RR
[M::process] read 706166 sequences (100000034 bp) in 2.315 CPU sec, 2.436 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 293696, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 205, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (228.72, 108.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 739)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704176 sequences (100000072 bp) in 1.430 CPU sec, 1.436 real sec...
[M::mem_process_seqs] Processed 700166 reads in 136.600 CPU sec, 4.336 real sec
[M::mem_process_seqs] Processed 706796 reads in 86.681 CPU sec, 2.740 real sec
sorting block 44, #reads = 1088784 mem = 536879688 peak = 1001828352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 293765, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 206, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595)
[M::mem_pestat] mean and std.dev: (230.40, 110.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 744)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703100 sequences (100000101 bp) in 1.968 CPU sec, 2.068 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 293344, 4, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 161, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559)
[M::mem_pestat] mean and std.dev: (161.56, 63.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 696)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (236.19, 113.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 702198 sequences (100000295 bp) in 1.430 CPU sec, 1.437 real sec...
[M::mem_process_seqs] Processed 706166 reads in 133.166 CPU sec, 4.236 real sec
[M::mem_process_seqs] Processed 704176 reads in 82.692 CPU sec, 2.614 real sec
sorting block 45, #reads = 1096272 mem = 536879327 peak = 1001828352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292527, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (239.87, 116.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701604 sequences (100000002 bp) in 2.234 CPU sec, 2.329 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292587, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (243.07, 118.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703100 reads in 129.091 CPU sec, 4.097 real sec
[M::process] read 701038 sequences (100000253 bp) in 1.497 CPU sec, 1.499 real sec...
sorting block 46, #reads = 1094187 mem = 536883777 peak = 1001828352
[M::mem_process_seqs] Processed 702198 reads in 81.493 CPU sec, 2.573 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292660, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (245.60, 120.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 47, #reads = 1094851 mem = 536881221 peak = 1060089856
[M::process] read 700910 sequences (100000170 bp) in 1.966 CPU sec, 2.071 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292103, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (247.32, 121.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701604 reads in 131.289 CPU sec, 4.177 real sec
[M::process] read 699218 sequences (100000156 bp) in 1.517 CPU sec, 1.522 real sec...
[M::mem_process_seqs] Processed 701038 reads in 83.404 CPU sec, 2.633 real sec
sorting block 48, #reads = 1094630 mem = 536878498 peak = 1060089856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291658, 3, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (246.80, 121.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 480, 1170)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3086)
[M::mem_pestat] mean and std.dev: (687.07, 549.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4044)
[M::mem_pestat] skip orientation RR
[M::process] read 698652 sequences (100000133 bp) in 2.334 CPU sec, 2.468 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291457, 2, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (387, 1237, 1853)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4785)
[M::mem_pestat] mean and std.dev: (1376.92, 1163.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6251)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (249.01, 120.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 439, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (334.67, 113.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 697968 sequences (100000173 bp) in 1.426 CPU sec, 1.423 real sec...
[M::mem_process_seqs] Processed 700910 reads in 133.025 CPU sec, 4.220 real sec
[M::mem_process_seqs] Processed 699218 reads in 85.485 CPU sec, 2.698 real sec
sorting block 49, #reads = 1093016 mem = 536875593 peak = 1060089856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290884, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.15, 120.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698526 sequences (100000107 bp) in 1.965 CPU sec, 2.078 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290610, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.60, 120.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698596 sequences (100000283 bp) in 1.440 CPU sec, 1.433 real sec...
[M::mem_process_seqs] Processed 698652 reads in 134.080 CPU sec, 4.267 real sec
sorting block 50, #reads = 1091402 mem = 536878908 peak = 1060089856
[M::mem_process_seqs] Processed 697968 reads in 84.427 CPU sec, 2.667 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290956, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (249.00, 119.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 51, #reads = 1090742 mem = 536882313 peak = 1060089856
[M::process] read 698566 sequences (100000192 bp) in 2.302 CPU sec, 2.399 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290651, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (247.91, 118.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698100 sequences (100000279 bp) in 1.430 CPU sec, 1.426 real sec...
[M::mem_process_seqs] Processed 698526 reads in 134.593 CPU sec, 4.270 real sec
[M::mem_process_seqs] Processed 698596 reads in 85.913 CPU sec, 2.715 real sec
sorting block 52, #reads = 1090892 mem = 536884844 peak = 1060089856
[M::process] read 698168 sequences (100000113 bp) in 2.042 CPU sec, 2.153 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290644, 8, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (230, 336, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (300.80, 129.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.11, 119.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 706782 sequences (100000290 bp) in 1.470 CPU sec, 1.474 real sec...
[M::mem_process_seqs] Processed 698100 reads in 77.268 CPU sec, 2.443 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290994, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 53, #reads = 1090414 mem = 536883949 peak = 1060089856
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.79, 119.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290708, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.20, 120.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698566 reads in 209.587 CPU sec, 6.659 real sec
[M::process] read 707462 sequences (100000042 bp) in 1.692 CPU sec, 1.695 real sec...
[M::mem_process_seqs] Processed 698168 reads in 100.661 CPU sec, 3.203 real sec
sorting block 54, #reads = 1089678 mem = 536876723 peak = 1060089856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 293359, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 205, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (228.25, 108.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 735)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 706972 sequences (100000253 bp) in 2.469 CPU sec, 2.613 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 293882, 10, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 204, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587)
[M::mem_pestat] mean and std.dev: (227.21, 107.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 734)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 856, 2743)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7921)
[M::mem_pestat] mean and std.dev: (1503.20, 1483.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10510)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 705546 sequences (100000244 bp) in 1.448 CPU sec, 1.450 real sec...
[M::mem_process_seqs] Processed 706782 reads in 148.011 CPU sec, 4.699 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 293917, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 205, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (229.12, 108.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 739)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704588 sequences (100000248 bp) in 1.846 CPU sec, 1.846 real sec...
[M::mem_process_seqs] Processed 706972 reads in 81.401 CPU sec, 2.577 real sec
sorting block 55, #reads = 1088675 mem = 536880106 peak = 1060089856
[M::mem_process_seqs] Processed 707462 reads in 194.162 CPU sec, 6.159 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 293315, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 208, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602)
[M::mem_pestat] mean and std.dev: (232.44, 110.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 753)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 56, #reads = 1089713 mem = 536883098 peak = 1085501440
[M::process] read 702968 sequences (100000093 bp) in 2.312 CPU sec, 2.435 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292866, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 210, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (235.47, 113.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 410, 1245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3209)
[M::mem_pestat] mean and std.dev: (507.80, 436.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4191)
[M::mem_pestat] skip orientation RR
[M::process] read 702088 sequences (100000056 bp) in 1.410 CPU sec, 1.407 real sec...
[M::mem_process_seqs] Processed 705546 reads in 158.337 CPU sec, 5.029 real sec
[M::mem_process_seqs] Processed 704588 reads in 91.363 CPU sec, 2.890 real sec
sorting block 57, #reads = 1090281 mem = 536883395 peak = 1085501440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 293167, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (239.42, 115.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701788 sequences (100000123 bp) in 2.297 CPU sec, 2.448 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291977, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (241.30, 116.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (128, 176, 264)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536)
[M::mem_pestat] mean and std.dev: (215.80, 112.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 672)
[M::mem_pestat] skip orientation RR
[M::process] read 701202 sequences (100000232 bp) in 1.450 CPU sec, 1.446 real sec...
[M::mem_process_seqs] Processed 702968 reads in 136.890 CPU sec, 4.340 real sec
[M::mem_process_seqs] Processed 702088 reads in 86.804 CPU sec, 2.744 real sec
sorting block 58, #reads = 1092120 mem = 536879873 peak = 1085501440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292597, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (242.88, 117.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700300 sequences (100000143 bp) in 2.343 CPU sec, 2.474 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291849, 8, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (244.60, 119.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 218, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (234.67, 105.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RR
[M::process] read 699264 sequences (100000035 bp) in 1.420 CPU sec, 1.417 real sec...
[M::mem_process_seqs] Processed 701788 reads in 134.390 CPU sec, 4.262 real sec
sorting block 59, #reads = 1091849 mem = 536877423 peak = 1085501440
[M::mem_process_seqs] Processed 701202 reads in 85.377 CPU sec, 2.695 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291768, 9, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (246.51, 119.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 60, #reads = 1092208 mem = 536877967 peak = 1100197888
[M::process] read 699018 sequences (100000163 bp) in 2.257 CPU sec, 2.380 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291133, 5, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (248.18, 120.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 548, 548)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1262)
[M::mem_pestat] mean and std.dev: (443.15, 285.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1619)
[M::mem_pestat] skip orientation RR
[M::process] read 698894 sequences (100000296 bp) in 1.450 CPU sec, 1.447 real sec...
[M::mem_process_seqs] Processed 700300 reads in 134.161 CPU sec, 4.256 real sec
[M::mem_process_seqs] Processed 699264 reads in 85.119 CPU sec, 2.690 real sec
sorting block 61, #reads = 1092074 mem = 536880794 peak = 1100197888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291047, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.25, 120.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698764 sequences (100000050 bp) in 2.212 CPU sec, 2.356 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290769, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.66, 120.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699082 sequences (100000176 bp) in 1.420 CPU sec, 1.422 real sec...
[M::mem_process_seqs] Processed 699018 reads in 133.716 CPU sec, 4.245 real sec
[M::mem_process_seqs] Processed 698894 reads in 85.167 CPU sec, 2.690 real sec
sorting block 62, #reads = 1090649 mem = 536880142 peak = 1100197888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291277, 4, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.99, 120.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 284, 985)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2569)
[M::mem_pestat] mean and std.dev: (454.58, 343.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3361)
[M::mem_pestat] skip orientation RR
[M::process] read 698576 sequences (100000015 bp) in 2.286 CPU sec, 2.372 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291170, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (247.66, 120.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 706662 sequences (100000191 bp) in 1.440 CPU sec, 1.448 real sec...
[M::mem_process_seqs] Processed 698764 reads in 135.282 CPU sec, 4.291 real sec
sorting block 63, #reads = 1090176 mem = 536877419 peak = 1100197888
[M::mem_process_seqs] Processed 699082 reads in 86.655 CPU sec, 2.736 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290450, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.86, 120.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 64, #reads = 1090815 mem = 536879115 peak = 1100197888
[M::process] read 707102 sequences (100000201 bp) in 2.388 CPU sec, 2.513 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 293449, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 206, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (229.66, 109.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 747)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704968 sequences (100000169 bp) in 1.420 CPU sec, 1.413 real sec...
[M::mem_process_seqs] Processed 698576 reads in 144.905 CPU sec, 4.600 real sec
[M::mem_process_seqs] Processed 706662 reads in 89.839 CPU sec, 2.848 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 294075, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 65, #reads = 1085379 mem = 536880458 peak = 1100197888
[M::mem_pestat] (25, 50, 75) percentile: (147, 206, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (228.87, 108.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 739)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704378 sequences (100000247 bp) in 2.514 CPU sec, 2.681 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 293374, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 209, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605)
[M::mem_pestat] mean and std.dev: (233.08, 111.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 757)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 707102 reads in 134.298 CPU sec, 4.262 real sec
[M::process] read 702216 sequences (100000165 bp) in 1.491 CPU sec, 1.499 real sec...
[M::mem_process_seqs] Processed 704968 reads in 85.121 CPU sec, 2.686 real sec
sorting block 66, #reads = 1095164 mem = 536877824 peak = 1100197888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 293588, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 210, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (235.14, 112.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702250 sequences (100000262 bp) in 2.341 CPU sec, 2.476 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292508, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (240.65, 115.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 704378 reads in 128.970 CPU sec, 4.094 real sec
[M::process] read 701694 sequences (100000136 bp) in 1.506 CPU sec, 1.513 real sec...
[M::mem_process_seqs] Processed 702216 reads in 81.353 CPU sec, 2.567 real sec
sorting block 67, #reads = 1095695 mem = 536879011 peak = 1100197888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292629, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (240.17, 115.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701372 sequences (100000098 bp) in 2.270 CPU sec, 2.378 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292208, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (241.72, 115.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702250 reads in 127.649 CPU sec, 4.056 real sec
[M::process] read 700962 sequences (100000164 bp) in 1.543 CPU sec, 1.561 real sec...
sorting block 68, #reads = 1094431 mem = 536879663 peak = 1100197888
[M::mem_process_seqs] Processed 701694 reads in 81.465 CPU sec, 2.564 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292883, 7, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (242.13, 115.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 794)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 69, #reads = 1095688 mem = 536877606 peak = 1100197888
[M::process] read 701826 sequences (100000212 bp) in 2.246 CPU sec, 2.356 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292285, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (243.00, 116.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701372 reads in 130.871 CPU sec, 4.162 real sec
[M::process] read 701928 sequences (100000042 bp) in 1.438 CPU sec, 1.443 real sec...
[M::mem_process_seqs] Processed 700962 reads in 83.227 CPU sec, 2.633 real sec
sorting block 70, #reads = 1094488 mem = 536880590 peak = 1100197888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 292263, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (241.02, 115.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701178 sequences (100000047 bp) in 2.165 CPU sec, 2.288 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292174, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 215, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627)
[M::mem_pestat] mean and std.dev: (239.92, 114.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701826 reads in 131.131 CPU sec, 4.174 real sec
[M::process] read 700068 sequences (100000013 bp) in 1.575 CPU sec, 1.598 real sec...
[M::mem_process_seqs] Processed 701928 reads in 83.521 CPU sec, 2.631 real sec
sorting block 71, #reads = 1092815 mem = 536886871 peak = 1100197888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291857, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (242.00, 116.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699878 sequences (100000142 bp) in 2.167 CPU sec, 2.269 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291536, 5, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (304, 550, 885)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2047)
[M::mem_pestat] mean and std.dev: (562.27, 300.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2628)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 219, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (245.49, 118.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 337, 1221)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3247)
[M::mem_pestat] mean and std.dev: (542.67, 427.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4260)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 699300 sequences (100000136 bp) in 1.410 CPU sec, 1.412 real sec...
[M::mem_process_seqs] Processed 701178 reads in 132.150 CPU sec, 4.198 real sec
sorting block 72, #reads = 1093796 mem = 536879417 peak = 1100197888
[M::mem_process_seqs] Processed 700068 reads in 83.542 CPU sec, 2.638 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291196, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (246.77, 119.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 73, #reads = 1092519 mem = 536879919 peak = 1100197888
[M::process] read 700030 sequences (100000074 bp) in 2.292 CPU sec, 2.388 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291235, 12, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.81, 120.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 473, 1673)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4699)
[M::mem_pestat] mean and std.dev: (909.50, 1024.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6212)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 705132 sequences (100000269 bp) in 1.500 CPU sec, 1.503 real sec...
[M::mem_process_seqs] Processed 699878 reads in 132.357 CPU sec, 4.196 real sec
[M::mem_process_seqs] Processed 699300 reads in 84.075 CPU sec, 2.656 real sec
sorting block 74, #reads = 1091746 mem = 536886372 peak = 1100197888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290883, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 219, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638)
[M::mem_pestat] mean and std.dev: (243.94, 117.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 708880 sequences (100000039 bp) in 2.127 CPU sec, 2.229 real sec...
[M::process] read 709042 sequences (100000006 bp) in 1.470 CPU sec, 1.467 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 293085, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 209, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605)
[M::mem_pestat] mean and std.dev: (232.98, 111.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 757)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700030 reads in 145.615 CPU sec, 4.626 real sec
[M::mem_process_seqs] Processed 705132 reads in 96.063 CPU sec, 3.045 real sec
sorting block 75, #reads = 1088332 mem = 536878070 peak = 1100197888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 294501, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 201, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574)
[M::mem_pestat] mean and std.dev: (224.02, 105.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 717)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 707960 sequences (100000262 bp) in 2.312 CPU sec, 2.394 real sec...
[M::process] read 706342 sequences (100000134 bp) in 1.470 CPU sec, 1.477 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 294841, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 201, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574)
[M::mem_pestat] mean and std.dev: (223.88, 105.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 717)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 708880 reads in 144.823 CPU sec, 4.595 real sec
[M::mem_process_seqs] Processed 709042 reads in 93.263 CPU sec, 2.954 real sec
sorting block 76, #reads = 1088722 mem = 536879175 peak = 1100197888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 294043, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 204, 292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584)
[M::mem_pestat] mean and std.dev: (226.81, 107.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704574 sequences (100000251 bp) in 2.385 CPU sec, 2.525 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 294028, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 206, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595)
[M::mem_pestat] mean and std.dev: (230.37, 109.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 744)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703742 sequences (100000010 bp) in 1.480 CPU sec, 1.474 real sec...
[M::mem_process_seqs] Processed 707960 reads in 145.546 CPU sec, 4.618 real sec
sorting block 77, #reads = 1092977 mem = 536884750 peak = 1100197888
[M::mem_process_seqs] Processed 706342 reads in 92.807 CPU sec, 2.938 real sec
sorting block 78, #reads = 1089931 mem = 536884546 peak = 1100197888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 293513, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (235.65, 113.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703496 sequences (100000122 bp) in 2.299 CPU sec, 2.382 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292898, 7, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 212, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (237.62, 114.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702940 sequences (100000267 bp) in 1.440 CPU sec, 1.445 real sec...
[M::mem_process_seqs] Processed 704574 reads in 137.682 CPU sec, 4.368 real sec
[M::mem_process_seqs] Processed 703742 reads in 86.840 CPU sec, 2.743 real sec
sorting block 79, #reads = 1093164 mem = 536879731 peak = 1100197888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292613, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (238.84, 115.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702126 sequences (100000033 bp) in 2.365 CPU sec, 2.474 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292735, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (239.78, 116.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700932 sequences (100000040 bp) in 1.400 CPU sec, 1.400 real sec...
[M::mem_process_seqs] Processed 703496 reads in 134.928 CPU sec, 4.280 real sec
[M::mem_process_seqs] Processed 702940 reads in 85.399 CPU sec, 2.698 real sec
sorting block 80, #reads = 1092636 mem = 536878709 peak = 1100197888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292314, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (241.32, 116.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700876 sequences (100000026 bp) in 2.332 CPU sec, 2.450 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291964, 10, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (244.64, 118.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (325, 921, 921)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2113)
[M::mem_pestat] mean and std.dev: (643.20, 371.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2709)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 205, 264)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (27, 422)
[M::mem_pestat] mean and std.dev: (180.56, 60.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 501)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 699924 sequences (100000050 bp) in 1.460 CPU sec, 1.463 real sec...
[M::mem_process_seqs] Processed 702126 reads in 135.038 CPU sec, 4.282 real sec
sorting block 81, #reads = 1092874 mem = 536880482 peak = 1100197888
[M::mem_process_seqs] Processed 700932 reads in 85.243 CPU sec, 2.693 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292285, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (245.18, 118.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 82, #reads = 1093137 mem = 536879840 peak = 1100197888
[M::process] read 699090 sequences (100000195 bp) in 2.536 CPU sec, 2.656 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291675, 5, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (249.17, 121.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 280, 924)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2434)
[M::mem_pestat] mean and std.dev: (455.21, 334.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3189)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700876 reads in 137.279 CPU sec, 4.360 real sec
[M::process] read 698774 sequences (100000261 bp) in 1.550 CPU sec, 1.569 real sec...
[M::mem_process_seqs] Processed 699924 reads in 88.157 CPU sec, 2.777 real sec
sorting block 83, #reads = 1093109 mem = 536881211 peak = 1100197888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291132, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.50, 121.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698104 sequences (100000101 bp) in 2.337 CPU sec, 2.462 real sec...
[M::process] read 10414 sequences (1482372 bp) in 0.300 CPU sec, 0.294 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291551, 7, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (106, 151, 681)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1831)
[M::mem_pestat] mean and std.dev: (256.78, 254.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2406)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.73, 120.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699090 reads in 137.048 CPU sec, 4.392 real sec
[M::mem_process_seqs] Processed 698774 reads in 86.983 CPU sec, 2.782 real sec
sorting block 84, #reads = 1092006 mem = 536884058 peak = 1100197888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290686, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 4288, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 217, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (239.61, 113.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 782)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.92, 119.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 10414 reads in 1.473 CPU sec, 0.052 real sec
[M::mem_process_seqs] Processed 698104 reads in 76.797 CPU sec, 2.508 real sec
sorting block 85, #reads = 1091585 mem = 536880276 peak = 1140998144
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 93891304
overall: 70064025600 mem 10897.143 user 303.253 sys 370.717 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1047_JG-T1-TDNA-1_A23MHWWLT4_1.L006\tPU:L006\tSM:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tLB:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0001.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0001.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 370.763 sec; CPU: 11200.399 sec
sorting block 86, #reads = 378352 mem = 186555965 peak = 1140998144
uniq header count 1
merging 87 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 5197287669
output reads: 94370132
bam_mem_sort: 87 calls 34.289 user 0.674 sys 35.178 real
bam_write: 87 calls 30.587 user 0.956 sys 31.832 real
execute: 1 calls 20.789 user 0.817 sys 398.874 real
merge_files: 1 calls 20.775 user 0.788 sys 27.844 real
parse_chunk: 1382 calls 46.008 user 13.694 sys 60.711 real
read_chunk: 3733 calls 32.819 user 30.894 sys 324.422 real
sort_block: 1 calls 25.380 user 30.263 sys 312.046 real
write_chunk: 2349 calls 20.427 user 0.554 sys 27.036 real
overall: 2989293568 mem 1088.667 user 54.375 sys 398.894 real
ls: cannot access '*.cram': No such file or directory