Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/49/e0df3073c44643725e65b3b35c7f27/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/54/a46528562f43ea547c793801a73ffe/1136_27F-T1-TDNA-1_A23MHWWLT4_1/0001.1136_27F-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/49/e0df3073c44643725e65b3b35c7f27/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/54/a46528562f43ea547c793801a73ffe/1136_27F-T1-TDNA-1_A23MHWWLT4_1/0001.1136_27F-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
==> STAGING COMPLETE (7 inputs)

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_27F-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
Received only 12 licenses out of 31 requested for sentieon:klib
[M::main_mem] bwa index loaded in 1642.900 CPU sec, 1052.758 real sec
[M::process] read 700894 sequences (100000042 bp) in 0.992 CPU sec, 0.992 real sec...
[M::process] read 700564 sequences (100000280 bp) in 1.220 CPU sec, 1.221 real sec...
[M::process] read 699786 sequences (100000169 bp) in 1.169 CPU sec, 1.169 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 291863, 17, 62)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 341, 816)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2028)
[M::mem_pestat] mean and std.dev: (393.70, 309.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2634)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 234, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (258.97, 129.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (242, 983, 2201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6119)
[M::mem_pestat] mean and std.dev: (1108.35, 904.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8078)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 281, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (327.53, 176.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 699696 sequences (100000083 bp) in 1.140 CPU sec, 1.140 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 291901, 14, 63)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 206, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (257.00, 157.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 239, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (264.61, 133.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 1088, 1732)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4870)
[M::mem_pestat] mean and std.dev: (837.08, 653.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6439)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 294, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (294.07, 179.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 292337, 28, 58)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 315, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044)
[M::mem_pestat] mean and std.dev: (304.22, 184.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1335)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 236, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (260.86, 129.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (237, 526, 1037)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2637)
[M::mem_pestat] mean and std.dev: (679.92, 615.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3437)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 333, 612)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1388)
[M::mem_pestat] mean and std.dev: (419.48, 288.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1776)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700894 reads in 200.503 CPU sec, 6.370 real sec
[M::mem_process_seqs] Processed 699786 reads in 133.234 CPU sec, 4.275 real sec
[M::mem_process_seqs] Processed 700564 reads in 145.045 CPU sec, 4.669 real sec
sorting block 0, #reads = 1090523 mem = 536881891 peak = 616673280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 291728, 25, 62)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 250, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (283.36, 183.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 238, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (264.65, 133.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::process] read 697104 sequences (100000181 bp) in 2.005 CPU sec, 2.152 real sec...
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (547, 1178, 1890)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4576)
[M::mem_pestat] mean and std.dev: (1100.80, 734.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5919)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 351, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1161)
[M::mem_pestat] mean and std.dev: (344.66, 193.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1478)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696254 sequences (100000190 bp) in 1.243 CPU sec, 1.258 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 291129, 32, 84)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (243, 398, 842)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2040)
[M::mem_pestat] mean and std.dev: (454.14, 370.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2639)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 248, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (273.32, 137.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (73, 741, 1430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4144)
[M::mem_pestat] mean and std.dev: (696.90, 638.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5501)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 306, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1174)
[M::mem_pestat] mean and std.dev: (379.11, 273.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1507)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699696 reads in 125.104 CPU sec, 4.037 real sec
sorting block 1, #reads = 1090466 mem = 536881081 peak = 847884288
[M::mem_process_seqs] Processed 697104 reads in 78.468 CPU sec, 2.473 real sec
[M::process] read 696622 sequences (100000122 bp) in 1.305 CPU sec, 1.337 real sec...
sorting block 2, #reads = 1090589 mem = 536882261 peak = 889937920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 290400, 30, 57)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 350, 669)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1627)
[M::mem_pestat] mean and std.dev: (422.11, 293.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2106)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 250, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (275.03, 137.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (304, 863, 1212)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3028)
[M::mem_pestat] mean and std.dev: (829.14, 589.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3936)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 308, 641)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1489)
[M::mem_pestat] mean and std.dev: (382.31, 244.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1913)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695146 sequences (100000008 bp) in 1.834 CPU sec, 1.897 real sec...
[M::process] read 694212 sequences (100000219 bp) in 1.200 CPU sec, 1.204 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 290729, 23, 59)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 290, 693)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1727)
[M::mem_pestat] mean and std.dev: (331.51, 224.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2244)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 248, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (272.32, 135.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (334, 856, 2035)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5437)
[M::mem_pestat] mean and std.dev: (1258.52, 1315.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7138)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 282, 601)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1435)
[M::mem_pestat] mean and std.dev: (369.22, 318.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1852)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696254 reads in 124.064 CPU sec, 3.939 real sec
[M::mem_process_seqs] Processed 696622 reads in 78.508 CPU sec, 2.494 real sec
sorting block 3, #reads = 1089002 mem = 536878956 peak = 889937920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 290638, 30, 65)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 265, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1154)
[M::mem_pestat] mean and std.dev: (337.41, 233.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1483)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 254, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (277.89, 138.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 560, 1021)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2731)
[M::mem_pestat] mean and std.dev: (600.63, 630.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3586)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 294, 589)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1327)
[M::mem_pestat] mean and std.dev: (368.82, 280.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1696)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 694432 sequences (100000229 bp) in 1.837 CPU sec, 1.943 real sec...
[M::process] read 693878 sequences (100000173 bp) in 1.230 CPU sec, 1.230 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (95, 289394, 27, 69)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 347, 584)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1414)
[M::mem_pestat] mean and std.dev: (387.00, 268.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1829)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 255, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (278.02, 136.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 578, 1112)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3042)
[M::mem_pestat] mean and std.dev: (495.04, 431.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4007)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 331, 540)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1264)
[M::mem_pestat] mean and std.dev: (342.64, 229.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1626)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695146 reads in 125.667 CPU sec, 4.002 real sec
[M::mem_process_seqs] Processed 694212 reads in 79.533 CPU sec, 2.536 real sec
sorting block 4, #reads = 1089216 mem = 536881256 peak = 889937920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 290259, 26, 71)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 305, 659)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1615)
[M::mem_pestat] mean and std.dev: (428.79, 326.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2093)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 253, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (275.99, 135.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (62, 765, 1242)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3602)
[M::mem_pestat] mean and std.dev: (875.08, 1012.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4927)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 299, 848)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2158)
[M::mem_pestat] mean and std.dev: (468.47, 403.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2813)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 693068 sequences (100000087 bp) in 1.911 CPU sec, 2.014 real sec...
[M::process] read 700498 sequences (100000255 bp) in 1.272 CPU sec, 1.282 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 289452, 29, 86)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 337, 782)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1988)
[M::mem_pestat] mean and std.dev: (487.84, 437.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2591)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 256, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (278.39, 136.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (27, 352, 839)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2463)
[M::mem_pestat] mean and std.dev: (422.52, 473.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3275)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 334, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1187)
[M::mem_pestat] mean and std.dev: (378.73, 259.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1514)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 694432 reads in 124.992 CPU sec, 3.971 real sec
sorting block 5, #reads = 1087320 mem = 536880008 peak = 889937920
[M::mem_process_seqs] Processed 693878 reads in 78.501 CPU sec, 2.494 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 289394, 25, 58)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 273, 751)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1935)
[M::mem_pestat] mean and std.dev: (355.31, 296.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2527)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 259, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (281.18, 137.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 561, 901)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2303)
[M::mem_pestat] mean and std.dev: (515.96, 347.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3004)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 420, 891)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2153)
[M::mem_pestat] mean and std.dev: (528.20, 391.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2784)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 6, #reads = 1086979 mem = 536881567 peak = 889937920
[M::process] read 700454 sequences (100000244 bp) in 2.028 CPU sec, 2.128 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 291949, 29, 63)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 293, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (257.17, 117.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 237, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (262.12, 131.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 327, 741)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1959)
[M::mem_pestat] mean and std.dev: (390.46, 306.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2568)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 345, 615)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1461)
[M::mem_pestat] mean and std.dev: (373.03, 260.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1884)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 699784 sequences (100000191 bp) in 1.310 CPU sec, 1.311 real sec...
[M::mem_process_seqs] Processed 693068 reads in 126.845 CPU sec, 4.031 real sec
[M::mem_process_seqs] Processed 700498 reads in 81.397 CPU sec, 2.576 real sec
sorting block 7, #reads = 1086832 mem = 536879550 peak = 889937920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 291414, 21, 77)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 307, 633)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1547)
[M::mem_pestat] mean and std.dev: (385.81, 284.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2004)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 236, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (262.46, 132.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 910)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (59, 493, 1091)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3155)
[M::mem_pestat] mean and std.dev: (660.40, 785.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4187)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 364, 1101)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2905)
[M::mem_pestat] mean and std.dev: (571.03, 558.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3807)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 699078 sequences (100000219 bp) in 2.128 CPU sec, 2.246 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 291938, 25, 69)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 319, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (302.96, 146.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 239, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (265.34, 133.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 716, 1258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3394)
[M::mem_pestat] mean and std.dev: (694.48, 639.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4462)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 277, 609)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1501)
[M::mem_pestat] mean and std.dev: (371.57, 319.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1947)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 697620 sequences (100000188 bp) in 1.340 CPU sec, 1.339 real sec...
[M::mem_process_seqs] Processed 700454 reads in 126.425 CPU sec, 4.015 real sec
[M::mem_process_seqs] Processed 699784 reads in 79.736 CPU sec, 2.526 real sec
sorting block 8, #reads = 1092962 mem = 536878579 peak = 889937920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 291304, 35, 55)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (223, 358, 598)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1348)
[M::mem_pestat] mean and std.dev: (409.54, 250.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1723)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 242, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (269.26, 136.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (236, 603, 1257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3299)
[M::mem_pestat] mean and std.dev: (820.53, 772.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4320)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 290, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (263.67, 115.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696910 sequences (100000205 bp) in 2.175 CPU sec, 2.282 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 291808, 51, 57)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 355, 1442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4008)
[M::mem_pestat] mean and std.dev: (461.95, 472.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5291)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 247, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (273.32, 138.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 956)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 337, 1285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3509)
[M::mem_pestat] mean and std.dev: (548.93, 567.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4621)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 337, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (310.11, 171.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1111)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695704 sequences (100000169 bp) in 1.360 CPU sec, 1.368 real sec...
[M::mem_process_seqs] Processed 699078 reads in 126.159 CPU sec, 4.008 real sec
[M::mem_process_seqs] Processed 697620 reads in 79.450 CPU sec, 2.511 real sec
sorting block 9, #reads = 1091420 mem = 536877751 peak = 889937920
[M::process] read 694666 sequences (100000199 bp) in 2.230 CPU sec, 2.355 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 290162, 29, 51)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 260, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (278.31, 158.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 252, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (276.75, 138.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 959)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (880, 1248, 2468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5644)
[M::mem_pestat] mean and std.dev: (1492.56, 1210.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7232)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 324, 571)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1221)
[M::mem_pestat] mean and std.dev: (387.75, 237.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1546)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 694258 sequences (100000167 bp) in 1.376 CPU sec, 1.383 real sec...
[M::mem_process_seqs] Processed 695704 reads in 71.969 CPU sec, 2.274 real sec
sorting block 10, #reads = 1090050 mem = 536887391 peak = 889937920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 290365, 16, 61)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 250, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (257.17, 153.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 250, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768)
[M::mem_pestat] mean and std.dev: (276.30, 139.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (110, 342, 778)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2114)
[M::mem_pestat] mean and std.dev: (414.44, 409.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2782)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 460, 837)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2109)
[M::mem_pestat] mean and std.dev: (554.28, 470.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2745)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 289802, 16, 72)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (199, 297, 664)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1594)
[M::mem_pestat] mean and std.dev: (393.11, 323.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2059)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 255, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (279.10, 138.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 962)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (232, 689, 1829)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5023)
[M::mem_pestat] mean and std.dev: (814.73, 787.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6620)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 352, 674)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1618)
[M::mem_pestat] mean and std.dev: (396.75, 317.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2090)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696910 reads in 195.441 CPU sec, 6.210 real sec
[M::process] read 694410 sequences (100000183 bp) in 1.680 CPU sec, 1.695 real sec...
[M::mem_process_seqs] Processed 694666 reads in 93.204 CPU sec, 2.956 real sec
sorting block 11, #reads = 1088143 mem = 536877788 peak = 932093952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 289523, 22, 62)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 268, 555)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1359)
[M::mem_pestat] mean and std.dev: (312.82, 251.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1761)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 256, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (279.30, 137.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 962)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (272, 1652, 2240)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6176)
[M::mem_pestat] mean and std.dev: (1264.45, 869.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8144)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 318, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (320.81, 137.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 694434 sequences (100000221 bp) in 2.285 CPU sec, 2.412 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 289566, 29, 83)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 232, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1092)
[M::mem_pestat] mean and std.dev: (283.12, 198.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1401)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 254, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (276.94, 136.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (384, 722, 1396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3420)
[M::mem_pestat] mean and std.dev: (915.86, 663.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4432)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 241, 670)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1652)
[M::mem_pestat] mean and std.dev: (381.68, 343.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2143)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 694258 reads in 127.588 CPU sec, 4.059 real sec
[M::process] read 694064 sequences (100000132 bp) in 1.385 CPU sec, 1.386 real sec...
sorting block 12, #reads = 1088156 mem = 536878907 peak = 932093952
[M::mem_process_seqs] Processed 694410 reads in 81.848 CPU sec, 2.588 real sec
sorting block 13, #reads = 1086211 mem = 536883963 peak = 932093952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 289594, 20, 75)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 323, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1244)
[M::mem_pestat] mean and std.dev: (329.48, 245.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1602)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 254, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (276.95, 136.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (93, 796, 1838)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5328)
[M::mem_pestat] mean and std.dev: (896.00, 799.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7073)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 255, 851)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2165)
[M::mem_pestat] mean and std.dev: (405.04, 351.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2822)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 699548 sequences (100000168 bp) in 2.225 CPU sec, 2.323 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (95, 289303, 23, 60)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 322, 590)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1428)
[M::mem_pestat] mean and std.dev: (369.09, 298.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1847)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 255, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (278.18, 136.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (233, 1228, 2421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6797)
[M::mem_pestat] mean and std.dev: (1866.70, 2313.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11122)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 319, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (289.55, 115.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 700958 sequences (100000281 bp) in 1.380 CPU sec, 1.379 real sec...
[M::mem_process_seqs] Processed 694434 reads in 129.352 CPU sec, 4.113 real sec
[M::mem_process_seqs] Processed 694064 reads in 82.137 CPU sec, 2.599 real sec
sorting block 14, #reads = 1086283 mem = 536878008 peak = 932093952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 291689, 33, 54)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 284, 803)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2087)
[M::mem_pestat] mean and std.dev: (410.22, 393.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2729)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 239, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (264.95, 133.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 724, 1688)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4758)
[M::mem_pestat] mean and std.dev: (1041.13, 1274.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6293)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 262, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879)
[M::mem_pestat] mean and std.dev: (270.91, 123.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 699822 sequences (100000175 bp) in 2.263 CPU sec, 2.350 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 292552, 33, 83)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 234, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (275.39, 182.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1302)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 236, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (261.67, 131.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 906)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (521, 831, 1715)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4103)
[M::mem_pestat] mean and std.dev: (1228.56, 1134.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5767)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 293, 854)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2170)
[M::mem_pestat] mean and std.dev: (524.96, 545.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2828)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 698768 sequences (100000215 bp) in 1.370 CPU sec, 1.376 real sec...
[M::mem_process_seqs] Processed 699548 reads in 128.935 CPU sec, 4.094 real sec
[M::mem_process_seqs] Processed 700958 reads in 81.782 CPU sec, 2.582 real sec
sorting block 15, #reads = 1087418 mem = 536879125 peak = 932093952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 291621, 19, 71)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 239, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (259.48, 130.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 239, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (264.53, 132.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (494, 994, 1817)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4463)
[M::mem_pestat] mean and std.dev: (866.41, 561.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5786)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 346, 589)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1355)
[M::mem_pestat] mean and std.dev: (368.69, 196.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1738)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 697530 sequences (100000028 bp) in 2.035 CPU sec, 2.160 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 291575, 29, 67)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 260, 806)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2022)
[M::mem_pestat] mean and std.dev: (398.73, 349.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2630)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 243, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (268.67, 135.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 934)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (360, 583, 1577)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4011)
[M::mem_pestat] mean and std.dev: (759.12, 655.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5228)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 347, 802)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2030)
[M::mem_pestat] mean and std.dev: (562.05, 520.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2644)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699822 reads in 127.005 CPU sec, 4.045 real sec
[M::process] read 697044 sequences (100000286 bp) in 1.470 CPU sec, 1.484 real sec...
[M::mem_process_seqs] Processed 698768 reads in 80.482 CPU sec, 2.541 real sec
sorting block 16, #reads = 1090683 mem = 536878442 peak = 932093952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 291397, 21, 60)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 263, 542)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1244)
[M::mem_pestat] mean and std.dev: (336.59, 234.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1595)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 246, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (273.20, 138.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 956)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (344, 565, 755)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1577)
[M::mem_pestat] mean and std.dev: (477.06, 259.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1988)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 259, 682)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1660)
[M::mem_pestat] mean and std.dev: (343.29, 227.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2149)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 17, #reads = 1088741 mem = 536877696 peak = 932093952
[M::process] read 696306 sequences (100000202 bp) in 2.301 CPU sec, 2.424 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 291016, 20, 60)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (276, 396, 610)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1278)
[M::mem_pestat] mean and std.dev: (388.21, 235.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1612)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 249, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759)
[M::mem_pestat] mean and std.dev: (275.05, 139.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (289, 972, 2665)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7417)
[M::mem_pestat] mean and std.dev: (1585.25, 1673.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9793)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 307, 1233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3311)
[M::mem_pestat] mean and std.dev: (712.14, 762.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4350)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697530 reads in 123.277 CPU sec, 3.922 real sec
[M::mem_process_seqs] Processed 697044 reads in 79.916 CPU sec, 2.518 real sec
[M::process] read 696400 sequences (100000161 bp) in 1.589 CPU sec, 1.622 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 290777, 27, 82)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 285, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1142)
[M::mem_pestat] mean and std.dev: (305.29, 176.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1458)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 250, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (275.92, 139.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 962)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (404, 729, 1350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3242)
[M::mem_pestat] mean and std.dev: (852.33, 620.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4188)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 245, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1138)
[M::mem_pestat] mean and std.dev: (281.67, 186.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1465)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 18, #reads = 1089672 mem = 536878271 peak = 932093952
[M::process] read 696170 sequences (100000004 bp) in 2.535 CPU sec, 2.678 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 290763, 34, 59)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 221, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886)
[M::mem_pestat] mean and std.dev: (244.20, 135.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 248, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (274.03, 137.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 465, 1241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3397)
[M::mem_pestat] mean and std.dev: (758.50, 761.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4475)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 257, 615)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1475)
[M::mem_pestat] mean and std.dev: (353.76, 295.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1905)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696306 reads in 125.193 CPU sec, 3.992 real sec
[M::mem_process_seqs] Processed 696400 reads in 81.373 CPU sec, 2.573 real sec
[M::process] read 695636 sequences (100000207 bp) in 2.263 CPU sec, 2.382 real sec...
sorting block 19, #reads = 1088777 mem = 536880693 peak = 932093952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 290526, 25, 77)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 267, 633)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1561)
[M::mem_pestat] mean and std.dev: (351.31, 287.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2025)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 248, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (272.69, 135.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 934)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (251, 617, 1006)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2516)
[M::mem_pestat] mean and std.dev: (524.68, 351.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3271)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 279, 662)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1592)
[M::mem_pestat] mean and std.dev: (372.65, 292.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2057)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 694750 sequences (100000208 bp) in 2.302 CPU sec, 2.448 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 290727, 25, 73)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 242, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1151)
[M::mem_pestat] mean and std.dev: (323.41, 267.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1479)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 251, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (274.50, 136.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (254, 1184, 1690)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4562)
[M::mem_pestat] mean and std.dev: (1094.52, 838.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5998)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 309, 543)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1309)
[M::mem_pestat] mean and std.dev: (363.43, 270.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1692)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696170 reads in 129.990 CPU sec, 4.160 real sec
[M::mem_process_seqs] Processed 695636 reads in 83.974 CPU sec, 2.659 real sec
sorting block 20, #reads = 1087857 mem = 536881121 peak = 932093952
[M::process] read 694376 sequences (100000039 bp) in 2.351 CPU sec, 2.502 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 289751, 23, 57)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 325, 850)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2150)
[M::mem_pestat] mean and std.dev: (455.43, 402.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2800)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 253, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (276.32, 136.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (203, 725, 1834)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5096)
[M::mem_pestat] mean and std.dev: (1165.83, 1183.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6727)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 282, 703)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1685)
[M::mem_pestat] mean and std.dev: (408.19, 273.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2176)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 699158 sequences (100000175 bp) in 1.949 CPU sec, 2.051 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 290202, 19, 73)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 332, 582)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1372)
[M::mem_pestat] mean and std.dev: (342.25, 217.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1767)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 254, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (277.15, 136.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (237, 608, 1045)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2661)
[M::mem_pestat] mean and std.dev: (620.06, 457.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3469)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 303, 627)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1491)
[M::mem_pestat] mean and std.dev: (406.69, 321.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1923)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 694750 reads in 130.438 CPU sec, 4.178 real sec
sorting block 21, #reads = 1087718 mem = 536877392 peak = 932093952
[M::mem_process_seqs] Processed 694376 reads in 83.989 CPU sec, 2.662 real sec
[M::process] read 700174 sequences (100000062 bp) in 1.874 CPU sec, 1.987 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 291457, 26, 51)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 270, 819)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2089)
[M::mem_pestat] mean and std.dev: (411.12, 384.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2724)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 240, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (266.00, 134.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 945, 1189)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3245)
[M::mem_pestat] mean and std.dev: (698.00, 508.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4273)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 241, 609)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1481)
[M::mem_pestat] mean and std.dev: (362.15, 284.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1917)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 22, #reads = 1086644 mem = 536881361 peak = 932093952
[M::process] read 699422 sequences (100000144 bp) in 1.920 CPU sec, 2.022 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (86, 291874, 31, 75)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 345, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1183)
[M::mem_pestat] mean and std.dev: (343.56, 212.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1513)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 238, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (264.04, 134.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (120, 268, 851)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2313)
[M::mem_pestat] mean and std.dev: (430.07, 437.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3044)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 341, 824)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1980)
[M::mem_pestat] mean and std.dev: (474.69, 384.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2558)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699158 reads in 132.503 CPU sec, 4.224 real sec
[M::process] read 698382 sequences (100000150 bp) in 1.439 CPU sec, 1.469 real sec...
[M::mem_process_seqs] Processed 700174 reads in 83.382 CPU sec, 2.637 real sec
sorting block 23, #reads = 1089415 mem = 536879504 peak = 932093952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 291819, 29, 75)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 262, 562)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1382)
[M::mem_pestat] mean and std.dev: (372.90, 298.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1792)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 241, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (266.83, 134.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (106, 435, 1075)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3013)
[M::mem_pestat] mean and std.dev: (543.24, 516.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3982)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 320, 762)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1822)
[M::mem_pestat] mean and std.dev: (477.39, 379.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2352)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 697616 sequences (100000284 bp) in 2.200 CPU sec, 2.299 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 290818, 26, 88)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 275, 841)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2255)
[M::mem_pestat] mean and std.dev: (504.14, 469.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2962)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 244, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (270.24, 136.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (628, 1098, 1892)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4420)
[M::mem_pestat] mean and std.dev: (1177.40, 702.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5684)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 277, 704)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1720)
[M::mem_pestat] mean and std.dev: (461.22, 432.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2228)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699422 reads in 128.875 CPU sec, 4.109 real sec
[M::process] read 696948 sequences (100000087 bp) in 1.406 CPU sec, 1.419 real sec...
sorting block 24, #reads = 1091708 mem = 536877519 peak = 932093952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 290596, 36, 65)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 265, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (267.98, 164.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 247, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (273.13, 138.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 691, 1413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3919)
[M::mem_pestat] mean and std.dev: (849.56, 883.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5172)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 325, 562)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1246)
[M::mem_pestat] mean and std.dev: (389.34, 245.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1588)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696770 sequences (100000076 bp) in 1.716 CPU sec, 1.722 real sec...
[M::mem_process_seqs] Processed 697616 reads in 71.182 CPU sec, 2.256 real sec
[M::mem_process_seqs] Processed 698382 reads in 174.845 CPU sec, 5.549 real sec
sorting block 25, #reads = 1089366 mem = 536879393 peak = 932093952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 290827, 29, 67)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (261, 347, 617)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1329)
[M::mem_pestat] mean and std.dev: (381.27, 229.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1685)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 248, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (274.82, 139.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (123, 371, 1175)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3279)
[M::mem_pestat] mean and std.dev: (586.43, 536.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4331)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 363, 770)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1886)
[M::mem_pestat] mean and std.dev: (452.02, 334.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2444)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 697118 sequences (100000102 bp) in 2.201 CPU sec, 2.325 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 290821, 16, 60)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 346, 834)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2168)
[M::mem_pestat] mean and std.dev: (497.13, 445.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2835)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 247, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (274.24, 139.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 351, 1768)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5020)
[M::mem_pestat] mean and std.dev: (829.12, 1182.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6646)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 335, 699)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1695)
[M::mem_pestat] mean and std.dev: (413.22, 330.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2193)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696948 reads in 142.043 CPU sec, 4.515 real sec
[M::mem_process_seqs] Processed 696770 reads in 83.365 CPU sec, 2.641 real sec
[M::process] read 697038 sequences (100000008 bp) in 1.554 CPU sec, 1.589 real sec...
sorting block 26, #reads = 1088841 mem = 536877317 peak = 937525248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 290762, 19, 73)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 300, 785)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1951)
[M::mem_pestat] mean and std.dev: (520.55, 426.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2534)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 245, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (271.87, 137.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (68, 271, 554)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1526)
[M::mem_pestat] mean and std.dev: (331.50, 341.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2012)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 311, 644)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1488)
[M::mem_pestat] mean and std.dev: (397.90, 302.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1910)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696698 sequences (100000033 bp) in 2.070 CPU sec, 2.175 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 290784, 23, 71)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 274, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (278.68, 162.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1304)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 245, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (270.55, 136.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (84, 428, 927)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2613)
[M::mem_pestat] mean and std.dev: (533.67, 608.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3456)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 328, 538)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1228)
[M::mem_pestat] mean and std.dev: (339.12, 193.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1573)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695908 sequences (100000237 bp) in 1.350 CPU sec, 1.347 real sec...
[M::mem_process_seqs] Processed 697118 reads in 129.811 CPU sec, 4.126 real sec
sorting block 27, #reads = 1089040 mem = 536877467 peak = 937525248
[M::mem_process_seqs] Processed 697038 reads in 82.610 CPU sec, 2.618 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 290797, 22, 70)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 247, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1226)
[M::mem_pestat] mean and std.dev: (318.66, 237.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1578)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 246, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (270.46, 134.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (220, 676, 1615)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4405)
[M::mem_pestat] mean and std.dev: (641.33, 549.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5800)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 266, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (298.82, 159.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 28, #reads = 1088818 mem = 536879970 peak = 937525248
[M::process] read 695010 sequences (100000130 bp) in 2.051 CPU sec, 2.156 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 289990, 34, 70)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 307, 611)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1507)
[M::mem_pestat] mean and std.dev: (418.97, 338.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1955)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 249, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (273.63, 136.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (240, 767, 1618)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4374)
[M::mem_pestat] mean and std.dev: (925.91, 725.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5752)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 334, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053)
[M::mem_pestat] mean and std.dev: (341.47, 203.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1341)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 700594 sequences (100000244 bp) in 1.450 CPU sec, 1.451 real sec...
[M::mem_process_seqs] Processed 696698 reads in 130.234 CPU sec, 4.141 real sec
[M::mem_process_seqs] Processed 695908 reads in 83.408 CPU sec, 2.640 real sec
sorting block 29, #reads = 1087142 mem = 536884303 peak = 937525248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 289829, 33, 99)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 219, 760)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1982)
[M::mem_pestat] mean and std.dev: (337.77, 351.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2593)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 251, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (275.60, 137.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (276, 606, 1744)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4680)
[M::mem_pestat] mean and std.dev: (839.53, 832.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6148)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 264, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (329.49, 204.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1319)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 704608 sequences (100000285 bp) in 2.342 CPU sec, 2.476 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 291701, 18, 63)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (199, 372, 778)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1936)
[M::mem_pestat] mean and std.dev: (446.81, 354.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2515)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 235, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (261.91, 132.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 902)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (441, 1326, 2018)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5172)
[M::mem_pestat] mean and std.dev: (1413.06, 1302.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6749)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 263, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (312.00, 211.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1312)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695010 reads in 133.493 CPU sec, 4.244 real sec
[M::process] read 702902 sequences (100000013 bp) in 1.400 CPU sec, 1.405 real sec...
[M::mem_process_seqs] Processed 700594 reads in 87.530 CPU sec, 2.771 real sec
sorting block 30, #reads = 1086970 mem = 536875492 peak = 937525248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 294029, 24, 52)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 290, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (274.77, 151.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 223, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (250.40, 127.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 856)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 713, 1587)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4365)
[M::mem_pestat] mean and std.dev: (911.08, 783.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5754)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 272, 588)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1396)
[M::mem_pestat] mean and std.dev: (317.66, 208.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1800)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 702408 sequences (100000024 bp) in 2.307 CPU sec, 2.430 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 293413, 22, 79)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (197, 312, 715)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1751)
[M::mem_pestat] mean and std.dev: (379.88, 289.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2269)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 228, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.08, 131.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 335, 932)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2466)
[M::mem_pestat] mean and std.dev: (365.00, 289.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3233)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 257, 678)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1664)
[M::mem_pestat] mean and std.dev: (395.24, 381.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2157)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 700486 sequences (100000178 bp) in 1.410 CPU sec, 1.410 real sec...
[M::mem_process_seqs] Processed 704608 reads in 135.349 CPU sec, 4.301 real sec
[M::mem_process_seqs] Processed 702902 reads in 84.731 CPU sec, 2.684 real sec
sorting block 31, #reads = 1089100 mem = 536879940 peak = 946810880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 293342, 15, 52)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 220, 630)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1612)
[M::mem_pestat] mean and std.dev: (334.67, 298.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2103)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 228, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (255.53, 130.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (255, 388, 709)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1617)
[M::mem_pestat] mean and std.dev: (423.60, 235.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2071)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 393, 789)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2075)
[M::mem_pestat] mean and std.dev: (498.10, 504.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2718)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 700012 sequences (100000051 bp) in 2.090 CPU sec, 2.175 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 292831, 28, 71)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 282, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (321.55, 203.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 235, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (264.00, 135.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 914)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (474, 865, 1437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3363)
[M::mem_pestat] mean and std.dev: (760.14, 488.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4326)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 279, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (284.35, 125.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 699746 sequences (100000268 bp) in 1.410 CPU sec, 1.410 real sec...
[M::mem_process_seqs] Processed 702408 reads in 134.711 CPU sec, 4.287 real sec
sorting block 32, #reads = 1091799 mem = 536878513 peak = 980545536
[M::mem_process_seqs] Processed 700486 reads in 84.339 CPU sec, 2.676 real sec
sorting block 33, #reads = 1090434 mem = 536878121 peak = 1004511232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 292424, 30, 69)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 327, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1198)
[M::mem_pestat] mean and std.dev: (337.24, 198.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1534)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 237, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (266.50, 137.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (335, 965, 1325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3305)
[M::mem_pestat] mean and std.dev: (860.07, 692.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4295)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 235, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (318.56, 222.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1326)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 698594 sequences (100000120 bp) in 2.265 CPU sec, 2.344 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 292136, 23, 62)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 261, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1112)
[M::mem_pestat] mean and std.dev: (305.63, 184.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1423)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 235, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (263.19, 133.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (299, 584, 2353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6461)
[M::mem_pestat] mean and std.dev: (1175.36, 1161.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8515)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 310, 676)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1646)
[M::mem_pestat] mean and std.dev: (382.88, 273.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2131)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696816 sequences (100000234 bp) in 1.400 CPU sec, 1.402 real sec...
[M::mem_process_seqs] Processed 700012 reads in 137.051 CPU sec, 4.371 real sec
[M::mem_process_seqs] Processed 699746 reads in 86.680 CPU sec, 2.759 real sec
sorting block 34, #reads = 1088490 mem = 536880687 peak = 1004511232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 291835, 17, 67)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (234, 299, 588)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1296)
[M::mem_pestat] mean and std.dev: (373.52, 272.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1650)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 241, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (269.63, 138.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (511, 753, 2715)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7123)
[M::mem_pestat] mean and std.dev: (1335.56, 1425.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9327)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 294, 648)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1568)
[M::mem_pestat] mean and std.dev: (430.30, 392.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2028)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695544 sequences (100000035 bp) in 2.348 CPU sec, 2.464 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 290872, 28, 71)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 292, 598)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1434)
[M::mem_pestat] mean and std.dev: (374.92, 273.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1852)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 248, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (273.80, 137.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (233, 776, 1321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3497)
[M::mem_pestat] mean and std.dev: (965.21, 848.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4585)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 265, 773)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1957)
[M::mem_pestat] mean and std.dev: (402.83, 355.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2549)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695352 sequences (100000110 bp) in 1.400 CPU sec, 1.393 real sec...
[M::mem_process_seqs] Processed 698594 reads in 134.476 CPU sec, 4.273 real sec
[M::mem_process_seqs] Processed 696816 reads in 86.487 CPU sec, 2.739 real sec
sorting block 35, #reads = 1088279 mem = 536882435 peak = 1004511232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 290355, 24, 81)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 257, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (287.78, 145.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 251, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (276.87, 138.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 959)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 1105, 2855)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8277)
[M::mem_pestat] mean and std.dev: (1618.12, 1566.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10988)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 351, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1138)
[M::mem_pestat] mean and std.dev: (355.36, 220.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1451)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 702046 sequences (100000027 bp) in 2.686 CPU sec, 2.871 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 290499, 35, 78)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 253, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1244)
[M::mem_pestat] mean and std.dev: (297.40, 188.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1608)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 252, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765)
[M::mem_pestat] mean and std.dev: (278.26, 139.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 964)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 607, 1155)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3051)
[M::mem_pestat] mean and std.dev: (670.21, 545.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3999)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 348, 615)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1441)
[M::mem_pestat] mean and std.dev: (387.16, 267.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1854)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695544 reads in 138.660 CPU sec, 4.411 real sec
[M::mem_process_seqs] Processed 695352 reads in 89.283 CPU sec, 2.820 real sec
sorting block 36, #reads = 1085207 mem = 536879441 peak = 1004511232
[M::process] read 707514 sequences (100000002 bp) in 1.663 CPU sec, 1.693 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 292470, 37, 53)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 276, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1240)
[M::mem_pestat] mean and std.dev: (309.15, 223.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1594)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 231, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (258.54, 131.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (205, 506, 1505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4105)
[M::mem_pestat] mean and std.dev: (835.95, 769.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5405)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 392, 1243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3407)
[M::mem_pestat] mean and std.dev: (714.34, 715.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4489)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 37, #reads = 1083891 mem = 536880366 peak = 1004511232
[M::process] read 704652 sequences (100000132 bp) in 2.303 CPU sec, 2.433 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 294563, 28, 71)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 315, 745)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1975)
[M::mem_pestat] mean and std.dev: (443.32, 458.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2590)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 216, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (241.75, 120.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (211, 512, 1216)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3226)
[M::mem_pestat] mean and std.dev: (705.86, 600.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4231)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 320, 578)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1272)
[M::mem_pestat] mean and std.dev: (348.58, 199.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1619)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 701922 sequences (100000016 bp) in 1.450 CPU sec, 1.450 real sec...
[M::mem_process_seqs] Processed 702046 reads in 147.509 CPU sec, 4.692 real sec
[M::mem_process_seqs] Processed 707514 reads in 95.170 CPU sec, 3.018 real sec
sorting block 38, #reads = 1085951 mem = 536881728 peak = 1004511232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 293852, 36, 65)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 315, 719)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1791)
[M::mem_pestat] mean and std.dev: (423.03, 364.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2327)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 223, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (250.31, 126.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 856)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 264, 1063)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2885)
[M::mem_pestat] mean and std.dev: (657.28, 679.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3796)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 390, 880)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2140)
[M::mem_pestat] mean and std.dev: (494.31, 395.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2770)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 701024 sequences (100000068 bp) in 2.564 CPU sec, 2.715 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 292821, 29, 54)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 229, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1179)
[M::mem_pestat] mean and std.dev: (364.18, 281.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1516)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 231, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705)
[M::mem_pestat] mean and std.dev: (258.01, 130.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (311, 592, 1001)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2381)
[M::mem_pestat] mean and std.dev: (562.74, 372.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3071)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 378, 557)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1299)
[M::mem_pestat] mean and std.dev: (390.15, 246.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1670)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 700518 sequences (100000094 bp) in 1.450 CPU sec, 1.450 real sec...
[M::mem_process_seqs] Processed 704652 reads in 144.673 CPU sec, 4.600 real sec
[M::mem_process_seqs] Processed 701922 reads in 92.383 CPU sec, 2.927 real sec
sorting block 39, #reads = 1087535 mem = 536879485 peak = 1004511232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 292620, 26, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (197, 329, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1049)
[M::mem_pestat] mean and std.dev: (353.33, 210.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1333)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 234, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718)
[M::mem_pestat] mean and std.dev: (261.78, 133.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 905)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 474, 1317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3605)
[M::mem_pestat] mean and std.dev: (550.78, 649.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4749)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (278, 517, 1970)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5354)
[M::mem_pestat] mean and std.dev: (1025.95, 1179.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7046)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 699460 sequences (100000099 bp) in 2.373 CPU sec, 2.502 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 292256, 24, 67)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 263, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1065)
[M::mem_pestat] mean and std.dev: (323.69, 222.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1362)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 236, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (263.65, 133.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 918)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (565, 770, 1242)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2596)
[M::mem_pestat] mean and std.dev: (892.65, 467.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3273)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 297, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (279.22, 91.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 698402 sequences (100000129 bp) in 1.490 CPU sec, 1.493 real sec...
[M::mem_process_seqs] Processed 701024 reads in 144.442 CPU sec, 4.589 real sec
sorting block 40, #reads = 1086583 mem = 536876088 peak = 1004511232
[M::mem_process_seqs] Processed 700518 reads in 91.096 CPU sec, 2.887 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 291926, 16, 89)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 302, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066)
[M::mem_pestat] mean and std.dev: (272.33, 180.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1369)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 239, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (266.61, 135.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 743, 1477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4075)
[M::mem_pestat] mean and std.dev: (740.60, 786.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5374)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 285, 536)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1184)
[M::mem_pestat] mean and std.dev: (364.17, 225.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1508)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 41, #reads = 1085436 mem = 536880166 peak = 1004511232
[M::process] read 697018 sequences (100000094 bp) in 2.754 CPU sec, 2.925 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 291818, 20, 73)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 272, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (274.54, 131.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 241, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (268.73, 136.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (221, 573, 1191)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3131)
[M::mem_pestat] mean and std.dev: (677.60, 613.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4101)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 348, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1098)
[M::mem_pestat] mean and std.dev: (336.56, 157.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1385)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699460 reads in 150.099 CPU sec, 4.775 real sec
[M::mem_process_seqs] Processed 698402 reads in 94.396 CPU sec, 2.980 real sec
[M::process] read 696360 sequences (100000247 bp) in 1.880 CPU sec, 1.915 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 290939, 27, 80)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 334, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1160)
[M::mem_pestat] mean and std.dev: (341.25, 214.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1493)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 248, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762)
[M::mem_pestat] mean and std.dev: (275.71, 140.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 436, 1239)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3437)
[M::mem_pestat] mean and std.dev: (622.58, 569.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4536)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 303, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1064)
[M::mem_pestat] mean and std.dev: (345.69, 209.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1352)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 42, #reads = 1085866 mem = 536878037 peak = 1004511232
[M::process] read 697032 sequences (100000220 bp) in 2.960 CPU sec, 3.142 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 291110, 16, 59)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 261, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1172)
[M::mem_pestat] mean and std.dev: (280.02, 187.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1500)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 247, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759)
[M::mem_pestat] mean and std.dev: (275.13, 140.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (222, 475, 1291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3429)
[M::mem_pestat] mean and std.dev: (503.43, 434.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4498)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 328, 682)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1582)
[M::mem_pestat] mean and std.dev: (473.16, 362.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2032)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697018 reads in 149.192 CPU sec, 4.762 real sec
[M::mem_process_seqs] Processed 696360 reads in 94.965 CPU sec, 3.009 real sec
[M::process] read 705502 sequences (100000094 bp) in 2.146 CPU sec, 2.264 real sec...
sorting block 43, #reads = 1085305 mem = 536877829 peak = 1004511232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 290487, 33, 77)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 253, 681)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1721)
[M::mem_pestat] mean and std.dev: (435.94, 403.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2241)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 244, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (271.83, 138.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 449, 1123)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3051)
[M::mem_pestat] mean and std.dev: (513.80, 520.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4015)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 465, 771)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1779)
[M::mem_pestat] mean and std.dev: (528.80, 391.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2283)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 702916 sequences (100000036 bp) in 2.562 CPU sec, 2.720 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 294313, 16, 61)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (209, 278, 562)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1268)
[M::mem_pestat] mean and std.dev: (344.28, 228.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1621)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 220, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (246.64, 124.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (316, 376, 1675)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4393)
[M::mem_pestat] mean and std.dev: (858.31, 841.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5752)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 469, 799)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1961)
[M::mem_pestat] mean and std.dev: (514.63, 417.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2542)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697032 reads in 158.863 CPU sec, 5.066 real sec
[M::mem_process_seqs] Processed 705502 reads in 98.120 CPU sec, 3.102 real sec
[M::process] read 701454 sequences (100000024 bp) in 1.747 CPU sec, 1.776 real sec...
sorting block 44, #reads = 1084447 mem = 536879303 peak = 1004511232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 292942, 16, 54)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 243, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (263.58, 142.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 229, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.31, 129.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (669, 1187, 1650)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3612)
[M::mem_pestat] mean and std.dev: (929.73, 604.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4593)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 275, 986)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2604)
[M::mem_pestat] mean and std.dev: (616.49, 625.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3413)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 700548 sequences (100000070 bp) in 2.483 CPU sec, 2.652 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 292734, 27, 70)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 306, 819)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2113)
[M::mem_pestat] mean and std.dev: (416.45, 483.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2760)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 232, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (259.99, 132.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 897)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (255, 402, 1464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3882)
[M::mem_pestat] mean and std.dev: (732.12, 647.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5091)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 281, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (333.75, 222.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1321)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 702916 reads in 146.473 CPU sec, 4.678 real sec
sorting block 45, #reads = 1086024 mem = 536879511 peak = 1004511232
[M::mem_process_seqs] Processed 701454 reads in 91.359 CPU sec, 2.891 real sec
[M::process] read 699652 sequences (100000034 bp) in 1.690 CPU sec, 1.727 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 292339, 11, 59)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 255, 606)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1446)
[M::mem_pestat] mean and std.dev: (370.75, 279.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1866)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 236, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (264.40, 135.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (828, 1805, 1886)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4002)
[M::mem_pestat] mean and std.dev: (1121.78, 694.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5060)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 230, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (299.78, 152.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1105)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 46, #reads = 1091124 mem = 536877835 peak = 1062010880
[M::process] read 698990 sequences (100000237 bp) in 2.550 CPU sec, 2.715 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 292012, 33, 63)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (224.79, 112.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 238, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (266.22, 135.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (66, 478, 634)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1770)
[M::mem_pestat] mean and std.dev: (407.07, 428.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2338)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 261, 625)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1515)
[M::mem_pestat] mean and std.dev: (313.25, 227.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1960)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700548 reads in 147.002 CPU sec, 4.692 real sec
[M::process] read 698466 sequences (100000102 bp) in 1.621 CPU sec, 1.638 real sec...
[M::mem_process_seqs] Processed 699652 reads in 92.929 CPU sec, 2.943 real sec
sorting block 47, #reads = 1089953 mem = 536880502 peak = 1062010880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 292059, 15, 72)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 278, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1099)
[M::mem_pestat] mean and std.dev: (295.70, 189.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1407)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 240, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (268.05, 136.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (554, 845, 1647)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3833)
[M::mem_pestat] mean and std.dev: (942.14, 595.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4926)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 273, 547)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1309)
[M::mem_pestat] mean and std.dev: (338.35, 272.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1690)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 697450 sequences (100000291 bp) in 2.541 CPU sec, 2.712 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 291481, 21, 80)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 345, 650)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1588)
[M::mem_pestat] mean and std.dev: (400.54, 368.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2057)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 242, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (270.28, 137.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (309, 458, 893)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2061)
[M::mem_pestat] mean and std.dev: (486.42, 295.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2645)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 293, 638)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1542)
[M::mem_pestat] mean and std.dev: (450.99, 434.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2189)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698990 reads in 147.908 CPU sec, 4.711 real sec
[M::process] read 697440 sequences (100000190 bp) in 1.529 CPU sec, 1.529 real sec...
[M::mem_process_seqs] Processed 698466 reads in 93.570 CPU sec, 2.964 real sec
sorting block 48, #reads = 1087454 mem = 536880753 peak = 1062010880
[M::process] read 697320 sequences (100000287 bp) in 2.732 CPU sec, 2.912 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 291305, 23, 69)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 243, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1130)
[M::mem_pestat] mean and std.dev: (337.95, 255.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1444)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 244, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (271.20, 137.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (104, 471, 934)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2594)
[M::mem_pestat] mean and std.dev: (534.41, 551.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3424)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 329, 934)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2378)
[M::mem_pestat] mean and std.dev: (489.48, 476.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3100)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 697048 sequences (100000084 bp) in 1.530 CPU sec, 1.531 real sec...
[M::mem_process_seqs] Processed 697440 reads in 88.064 CPU sec, 2.789 real sec
sorting block 49, #reads = 1086758 mem = 536878684 peak = 1062010880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 291091, 35, 75)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 248, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (247.34, 158.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 244, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (271.42, 137.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 674, 1732)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4910)
[M::mem_pestat] mean and std.dev: (946.14, 821.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6499)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 286, 660)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1622)
[M::mem_pestat] mean and std.dev: (355.23, 290.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2103)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 290822, 29, 80)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 301, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1223)
[M::mem_pestat] mean and std.dev: (298.87, 185.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1572)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 243, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (270.55, 136.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (447, 772, 3179)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8643)
[M::mem_pestat] mean and std.dev: (1607.69, 1828.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11375)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 312, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1115)
[M::mem_pestat] mean and std.dev: (311.23, 194.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1425)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697450 reads in 234.758 CPU sec, 7.470 real sec
[M::process] read 706908 sequences (100000121 bp) in 1.603 CPU sec, 1.608 real sec...
[M::mem_process_seqs] Processed 697320 reads in 110.025 CPU sec, 3.508 real sec
sorting block 50, #reads = 1085391 mem = 536880695 peak = 1062010880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 290185, 21, 85)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 277, 560)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1308)
[M::mem_pestat] mean and std.dev: (339.75, 247.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1682)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 244, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (271.17, 137.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (460, 686, 1744)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4312)
[M::mem_pestat] mean and std.dev: (1002.55, 779.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5596)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 303, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060)
[M::mem_pestat] mean and std.dev: (296.91, 160.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1354)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 707692 sequences (100000043 bp) in 2.745 CPU sec, 2.906 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 293936, 18, 74)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (211, 288, 554)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1240)
[M::mem_pestat] mean and std.dev: (328.77, 209.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1583)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 217, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (243.20, 122.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (914, 1286, 1773)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3491)
[M::mem_pestat] mean and std.dev: (1201.18, 668.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4350)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 244, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1180)
[M::mem_pestat] mean and std.dev: (256.25, 199.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1521)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 703922 sequences (100000107 bp) in 1.530 CPU sec, 1.532 real sec...
[M::mem_process_seqs] Processed 697048 reads in 163.632 CPU sec, 5.206 real sec
sorting block 51, #reads = 1084356 mem = 536880124 peak = 1062010880
[M::mem_process_seqs] Processed 706908 reads in 106.266 CPU sec, 3.376 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 294640, 21, 61)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 296, 754)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1908)
[M::mem_pestat] mean and std.dev: (405.59, 324.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2485)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 217, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (242.49, 121.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (277, 633, 939)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2263)
[M::mem_pestat] mean and std.dev: (687.62, 511.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2925)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 250, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (306.47, 192.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 52, #reads = 1081510 mem = 536878955 peak = 1062010880
[M::process] read 702230 sequences (100000017 bp) in 2.527 CPU sec, 2.690 real sec...
[M::process] read 700758 sequences (100000135 bp) in 1.560 CPU sec, 1.559 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 293787, 30, 48)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 314, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (273.08, 136.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 225, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687)
[M::mem_pestat] mean and std.dev: (251.84, 126.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 865)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (248, 580, 1292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3380)
[M::mem_pestat] mean and std.dev: (728.00, 620.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4424)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 266, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (246.10, 105.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 707692 reads in 160.672 CPU sec, 5.111 real sec
[M::mem_process_seqs] Processed 703922 reads in 98.864 CPU sec, 3.136 real sec
sorting block 53, #reads = 1085674 mem = 536878216 peak = 1062010880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 292667, 17, 58)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 301, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1226)
[M::mem_pestat] mean and std.dev: (329.71, 210.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1576)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 232, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (258.97, 131.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (205, 742, 2455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6955)
[M::mem_pestat] mean and std.dev: (1164.65, 1120.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9205)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 374, 538)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1086)
[M::mem_pestat] mean and std.dev: (393.63, 235.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1360)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 699864 sequences (100000268 bp) in 2.487 CPU sec, 2.619 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 292112, 19, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 254, 612)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1528)
[M::mem_pestat] mean and std.dev: (364.02, 321.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1986)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 236, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (263.43, 134.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 914)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (432, 541, 1555)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3801)
[M::mem_pestat] mean and std.dev: (1010.47, 944.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4924)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 330, 721)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1729)
[M::mem_pestat] mean and std.dev: (428.77, 338.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2233)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 699092 sequences (100000176 bp) in 1.510 CPU sec, 1.509 real sec...
[M::mem_process_seqs] Processed 702230 reads in 153.674 CPU sec, 4.893 real sec
[M::mem_process_seqs] Processed 700758 reads in 96.902 CPU sec, 3.072 real sec
sorting block 54, #reads = 1086097 mem = 536877516 peak = 1062010880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 291744, 29, 70)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 245, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1071)
[M::mem_pestat] mean and std.dev: (298.02, 206.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1373)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 236, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (264.63, 135.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 494, 1952)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5518)
[M::mem_pestat] mean and std.dev: (1120.90, 1371.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7301)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 313, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (310.69, 150.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 698934 sequences (100000156 bp) in 2.504 CPU sec, 2.685 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 291782, 29, 64)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 183, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (198.40, 98.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 804)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 238, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (266.10, 135.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (251, 754, 1843)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5027)
[M::mem_pestat] mean and std.dev: (841.69, 645.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6619)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (289, 406, 1221)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3085)
[M::mem_pestat] mean and std.dev: (809.44, 771.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4017)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 698026 sequences (100000152 bp) in 1.470 CPU sec, 1.469 real sec...
[M::mem_process_seqs] Processed 699864 reads in 152.703 CPU sec, 4.861 real sec
[M::mem_process_seqs] Processed 699092 reads in 95.351 CPU sec, 3.029 real sec
sorting block 55, #reads = 1085761 mem = 536879475 peak = 1062010880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 291970, 22, 69)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 290, 962)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2564)
[M::mem_pestat] mean and std.dev: (455.37, 425.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3365)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 239, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (266.57, 135.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (330, 842, 1723)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4509)
[M::mem_pestat] mean and std.dev: (802.47, 772.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5902)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 269, 550)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1290)
[M::mem_pestat] mean and std.dev: (354.02, 259.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1660)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 697138 sequences (100000006 bp) in 2.476 CPU sec, 2.605 real sec...
[M::process] read 581082 sequences (83370225 bp) in 1.290 CPU sec, 1.284 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 291374, 23, 69)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 263, 1122)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2966)
[M::mem_pestat] mean and std.dev: (638.65, 639.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3888)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 242, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (268.97, 136.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 930)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 325, 951)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2483)
[M::mem_pestat] mean and std.dev: (479.61, 464.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3249)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 257, 681)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1683)
[M::mem_pestat] mean and std.dev: (409.88, 337.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2184)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698934 reads in 147.120 CPU sec, 4.687 real sec
sorting block 56, #reads = 1085070 mem = 536882673 peak = 1062010880
[M::mem_process_seqs] Processed 698026 reads in 91.464 CPU sec, 2.908 real sec
sorting block 57, #reads = 1086941 mem = 536880596 peak = 1116393472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 290839, 30, 64)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 246, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046)
[M::mem_pestat] mean and std.dev: (276.75, 176.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1340)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 244, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (271.54, 137.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (90, 532, 1196)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3408)
[M::mem_pestat] mean and std.dev: (633.50, 613.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4514)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 246, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1100)
[M::mem_pestat] mean and std.dev: (322.39, 245.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1407)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 242892, 25, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (218, 317, 767)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1865)
[M::mem_pestat] mean and std.dev: (447.26, 337.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2414)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 245, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (272.28, 138.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (215, 254, 1086)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2828)
[M::mem_pestat] mean and std.dev: (406.14, 400.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3699)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 227, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (227.94, 116.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 967)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697138 reads in 131.555 CPU sec, 4.272 real sec
[M::mem_process_seqs] Processed 581082 reads in 77.248 CPU sec, 2.509 real sec
sorting block 58, #reads = 1086920 mem = 536879520 peak = 1116393472
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 64156528
overall: 69971197952 mem 8216.811 user 313.330 sys 1281.857 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_27F-T1-TDNA-1_A23MHWWLT4_1.L003\tPU:L003\tSM:1136_27F-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_27F-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0001.1136_27F-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0001.1136_27F-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 1281.894 sec; CPU: 8530.144 sec
sorting block 59, #reads = 516006 mem = 255299166 peak = 1116393472
uniq header count 1
merging 60 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3630295906
output reads: 64696324
bam_mem_sort: 60 calls 26.060 user 0.435 sys 26.599 real
bam_write: 60 calls 31.322 user 0.969 sys 32.478 real
execute: 1 calls 12.810 user 0.879 sys 1301.280 real
merge_files: 1 calls 12.796 user 0.856 sys 18.737 real
parse_chunk: 952 calls 33.596 user 8.656 sys 42.817 real
read_chunk: 2573 calls 24.356 user 23.582 sys 230.492 real
sort_block: 1 calls 19.008 user 22.926 sys 222.400 real
write_chunk: 1619 calls 12.427 user 0.682 sys 17.968 real
overall: 2747232256 mem 730.197 user 43.372 sys 1301.299 real
ls: cannot access '*.cram': No such file or directory