.command.log - Workdir - DAQ:SENTIEON_BWAMEM (FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1;L001;0001)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/cc/08d4fee30c2e6ca3bc83fac2b1dddd/.command.log
Size: 203.8 KB
Task: DAQ:SENTIEON_BWAMEM (FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1;L001;0001)
Attempt
Content

  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/cc/08d4fee30c2e6ca3bc83fac2b1dddd/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/07/6becf5d5568856c1b9f986d3d8bec9/FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1/FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1_R2.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/cc/08d4fee30c2e6ca3bc83fac2b1dddd/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/07/6becf5d5568856c1b9f986d3d8bec9/FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1/FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1_R1.fastq.gz
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1958.212 CPU sec, 91.850 real sec
[M::process] read 676182 sequences (100000245 bp) in 1.576 CPU sec, 1.576 real sec...
[M::process] read 675670 sequences (100000145 bp) in 1.801 CPU sec, 2.041 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281531, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 371, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (380.28, 169.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675118 sequences (100000290 bp) in 1.802 CPU sec, 2.019 real sec...
[M::process] read 674860 sequences (100000152 bp) in 1.838 CPU sec, 1.935 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281147, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 377, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (384.34, 168.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676182 reads in 192.981 CPU sec, 7.245 real sec
[M::mem_process_seqs] Processed 675670 reads in 123.301 CPU sec, 4.813 real sec
sorting block 0, #reads = 1064460 mem = 536882308 peak = 562532352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280918, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 382, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (388.63, 170.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674900 sequences (100000013 bp) in 2.378 CPU sec, 2.687 real sec...
[M::process] read 674610 sequences (100000235 bp) in 1.902 CPU sec, 1.993 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 279518, 0, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (637, 637, 637)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (637, 637)
[M::mem_pestat] mean and std.dev: (637.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (637, 637)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 385, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (392.93, 171.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675118 reads in 190.712 CPU sec, 6.761 real sec
[M::mem_process_seqs] Processed 674860 reads in 125.186 CPU sec, 4.647 real sec
sorting block 1, #reads = 1064611 mem = 536879832 peak = 696291328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280629, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 388, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (394.86, 172.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674378 sequences (100000002 bp) in 2.472 CPU sec, 2.941 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280131, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 387, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (394.39, 171.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674216 sequences (100000001 bp) in 2.028 CPU sec, 2.156 real sec...
[M::mem_process_seqs] Processed 674900 reads in 190.775 CPU sec, 6.674 real sec
[M::mem_process_seqs] Processed 674610 reads in 123.439 CPU sec, 4.401 real sec
sorting block 2, #reads = 1064695 mem = 536880607 peak = 798195712
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280070, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 389, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (395.64, 170.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673912 sequences (100000073 bp) in 2.736 CPU sec, 3.345 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280449, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 393, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (398.33, 171.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673758 sequences (100000027 bp) in 2.177 CPU sec, 2.237 real sec...
[M::mem_process_seqs] Processed 674378 reads in 202.486 CPU sec, 7.516 real sec
sorting block 3, #reads = 1063610 mem = 536880005 peak = 798195712
[M::mem_process_seqs] Processed 674216 reads in 130.548 CPU sec, 4.745 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280654, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 395, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (400.99, 172.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1280)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674592 sequences (100000064 bp) in 2.734 CPU sec, 3.218 real sec...
sorting block 4, #reads = 1063516 mem = 536880130 peak = 812855296
[M::process] read 674632 sequences (100000040 bp) in 2.107 CPU sec, 2.168 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279806, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 399, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (403.72, 173.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673912 reads in 209.663 CPU sec, 7.929 real sec
[M::mem_process_seqs] Processed 673758 reads in 133.116 CPU sec, 4.984 real sec
sorting block 5, #reads = 1063630 mem = 536878093 peak = 812855296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 280285, 1, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (379, 459, 596)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (475.79, 174.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 393, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (398.49, 173.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1291)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674576 sequences (100000241 bp) in 2.977 CPU sec, 3.654 real sec...
[M::process] read 674610 sequences (100000189 bp) in 2.148 CPU sec, 2.286 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280391, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 389, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (395.58, 173.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674592 reads in 223.113 CPU sec, 8.515 real sec
[M::mem_process_seqs] Processed 674632 reads in 140.198 CPU sec, 5.282 real sec
sorting block 6, #reads = 1064818 mem = 536878223 peak = 812855296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 279671, 0, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (254, 254, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (319.31, 153.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 389, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (396.52, 173.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674544 sequences (100000056 bp) in 2.734 CPU sec, 3.357 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 280328, 1, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (107, 270, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (197.60, 91.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 388, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (396.12, 173.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 674576 reads in 197.226 CPU sec, 7.186 real sec
[M::mem_process_seqs] Processed 674610 reads in 127.784 CPU sec, 4.599 real sec
[M::process] read 674324 sequences (100000078 bp) in 2.370 CPU sec, 2.812 real sec...
sorting block 7, #reads = 1064835 mem = 536879034 peak = 812855296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 279737, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 388, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (395.50, 173.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674598 sequences (100000200 bp) in 2.626 CPU sec, 3.109 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 279686, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 387, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (394.51, 171.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674324 sequences (100000181 bp) in 2.184 CPU sec, 2.312 real sec...
[M::mem_process_seqs] Processed 674544 reads in 196.408 CPU sec, 7.220 real sec
sorting block 8, #reads = 1065962 mem = 536880655 peak = 812855296
[M::mem_process_seqs] Processed 674324 reads in 127.251 CPU sec, 4.597 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279569, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 386, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (394.66, 172.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 9, #reads = 1065344 mem = 536878835 peak = 897548288
[M::process] read 674434 sequences (100000061 bp) in 2.681 CPU sec, 3.335 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280048, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 384, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (392.00, 170.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674598 reads in 193.672 CPU sec, 6.987 real sec
[M::mem_process_seqs] Processed 674324 reads in 126.167 CPU sec, 4.652 real sec
[M::process] read 674834 sequences (100000223 bp) in 2.805 CPU sec, 3.113 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280081, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 381, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (389.89, 169.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 10, #reads = 1064433 mem = 536880649 peak = 897548288
[M::process] read 676418 sequences (100000102 bp) in 3.436 CPU sec, 3.929 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280169, 2, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 375, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (383.67, 166.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (134, 340, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (273.60, 102.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 674434 reads in 192.335 CPU sec, 7.018 real sec
[M::mem_process_seqs] Processed 674834 reads in 125.817 CPU sec, 4.614 real sec
[M::process] read 674606 sequences (100000196 bp) in 2.814 CPU sec, 3.098 real sec...
sorting block 11, #reads = 1065449 mem = 536880792 peak = 897548288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281382, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 361, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (369.04, 160.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674698 sequences (100000278 bp) in 3.065 CPU sec, 3.791 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279841, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 376, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (385.04, 167.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676418 reads in 200.999 CPU sec, 6.845 real sec
[M::mem_process_seqs] Processed 674606 reads in 130.571 CPU sec, 4.402 real sec
sorting block 12, #reads = 1062661 mem = 536878080 peak = 897548288
[M::process] read 674484 sequences (100000057 bp) in 3.077 CPU sec, 3.495 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 279451, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 377, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (386.07, 168.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279635, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 377, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (387.02, 169.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674416 sequences (100000023 bp) in 3.153 CPU sec, 3.855 real sec...
[M::mem_process_seqs] Processed 674698 reads in 195.317 CPU sec, 6.755 real sec
sorting block 13, #reads = 1061996 mem = 536881021 peak = 897548288
[M::mem_process_seqs] Processed 674484 reads in 126.099 CPU sec, 4.358 real sec
[M::process] read 674350 sequences (100000245 bp) in 3.219 CPU sec, 3.716 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279387, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 377, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (386.60, 168.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674416 reads in 107.162 CPU sec, 3.745 real sec
sorting block 14, #reads = 1064013 mem = 536877643 peak = 897548288
[M::process] read 674320 sequences (100000093 bp) in 2.708 CPU sec, 3.061 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279396, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 377, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (386.64, 167.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279688, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 380, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (389.24, 169.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674350 reads in 191.811 CPU sec, 6.620 real sec
[M::process] read 674222 sequences (100000058 bp) in 3.109 CPU sec, 3.734 real sec...
[M::mem_process_seqs] Processed 674320 reads in 125.077 CPU sec, 4.344 real sec
sorting block 15, #reads = 1064826 mem = 536879594 peak = 897548288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280655, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 380, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (390.35, 170.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674222 reads in 102.113 CPU sec, 3.480 real sec
[M::process] read 674160 sequences (100000166 bp) in 3.373 CPU sec, 3.697 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279852, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 379, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (389.23, 169.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 16, #reads = 1064038 mem = 536881584 peak = 897548288
[M::mem_process_seqs] Processed 674160 reads in 98.581 CPU sec, 3.336 real sec
[M::process] read 673932 sequences (100000163 bp) in 3.221 CPU sec, 3.501 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280120, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 380, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (390.73, 170.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673786 sequences (100000116 bp) in 2.852 CPU sec, 3.021 real sec...
sorting block 17, #reads = 1063873 mem = 536880545 peak = 897548288
[M::mem_process_seqs] Processed 673932 reads in 96.180 CPU sec, 3.224 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279889, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 383, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (393.39, 171.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675276 sequences (100000043 bp) in 2.640 CPU sec, 2.844 real sec...
[M::mem_process_seqs] Processed 673786 reads in 96.726 CPU sec, 3.213 real sec
sorting block 18, #reads = 1064072 mem = 536881723 peak = 897548288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280691, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 675334 sequences (100000074 bp) in 2.903 CPU sec, 3.141 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (253, 376, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (385.40, 170.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280483, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 378, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (386.91, 170.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675016 sequences (100000282 bp) in 2.411 CPU sec, 2.472 real sec...
[M::mem_process_seqs] Processed 675276 reads in 172.592 CPU sec, 5.570 real sec
[M::mem_process_seqs] Processed 675334 reads in 112.782 CPU sec, 3.573 real sec
sorting block 19, #reads = 1064247 mem = 536878312 peak = 897548288
[M::process] read 674546 sequences (100000145 bp) in 2.947 CPU sec, 3.096 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280814, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 383, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (391.45, 172.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 20, #reads = 1065402 mem = 536879776 peak = 898179072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 279866, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 390, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (396.73, 173.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674268 sequences (100000024 bp) in 2.901 CPU sec, 3.112 real sec...
[M::mem_process_seqs] Processed 675016 reads in 170.345 CPU sec, 5.474 real sec
[M::mem_process_seqs] Processed 674546 reads in 110.821 CPU sec, 3.515 real sec
[M::process] read 673938 sequences (100000236 bp) in 3.037 CPU sec, 3.215 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279665, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 393, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (399.80, 174.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 21, #reads = 1066191 mem = 536877828 peak = 898179072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279980, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 396, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (403.20, 176.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674268 reads in 170.559 CPU sec, 5.474 real sec
[M::process] read 674104 sequences (100000032 bp) in 3.320 CPU sec, 3.579 real sec...
[M::mem_process_seqs] Processed 673938 reads in 110.359 CPU sec, 3.513 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 279977, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 395, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (401.07, 174.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674104 reads in 93.986 CPU sec, 3.131 real sec
sorting block 22, #reads = 1064700 mem = 536879644 peak = 898179072
[M::process] read 673838 sequences (100000170 bp) in 3.305 CPU sec, 3.520 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280013, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 397, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037)
[M::mem_pestat] mean and std.dev: (402.92, 174.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673838 reads in 94.856 CPU sec, 3.163 real sec
[M::process] read 673676 sequences (100000109 bp) in 3.340 CPU sec, 3.577 real sec...
sorting block 23, #reads = 1065275 mem = 536879829 peak = 898179072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279565, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 400, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (405.52, 174.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673676 reads in 95.937 CPU sec, 3.186 real sec
[M::process] read 673468 sequences (100000274 bp) in 3.093 CPU sec, 3.321 real sec...
sorting block 24, #reads = 1065346 mem = 536880469 peak = 898179072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279564, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 403, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044)
[M::mem_pestat] mean and std.dev: (408.35, 176.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1301)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673468 reads in 95.262 CPU sec, 3.120 real sec
[M::process] read 673582 sequences (100000118 bp) in 3.472 CPU sec, 3.723 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279664, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 402, 531)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1049)
[M::mem_pestat] mean and std.dev: (407.72, 175.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1308)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 25, #reads = 1064413 mem = 536881098 peak = 898179072
[M::mem_process_seqs] Processed 673582 reads in 96.371 CPU sec, 3.140 real sec
[M::process] read 674350 sequences (100000107 bp) in 3.450 CPU sec, 3.694 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280517, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 393, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (400.62, 175.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1296)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674388 sequences (100000073 bp) in 2.678 CPU sec, 2.762 real sec...
[M::mem_process_seqs] Processed 674350 reads in 90.420 CPU sec, 2.914 real sec
sorting block 26, #reads = 1064651 mem = 536878513 peak = 898179072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279612, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 391, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037)
[M::mem_pestat] mean and std.dev: (398.92, 175.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674388 reads in 94.359 CPU sec, 3.043 real sec
[M::process] read 674414 sequences (100000105 bp) in 3.097 CPU sec, 3.289 real sec...
sorting block 27, #reads = 1066004 mem = 536878516 peak = 898179072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280168, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 390, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (397.85, 174.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 265, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (77, 547)
[M::mem_pestat] mean and std.dev: (279.25, 48.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 641)
[M::mem_pestat] skip orientation RR
[M::process] read 674338 sequences (100000157 bp) in 2.876 CPU sec, 2.972 real sec...
[M::mem_process_seqs] Processed 674414 reads in 93.970 CPU sec, 3.018 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279737, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 389, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (397.18, 174.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674338 reads in 92.262 CPU sec, 2.967 real sec
[M::process] read 674368 sequences (100000088 bp) in 3.296 CPU sec, 3.460 real sec...
sorting block 28, #reads = 1064659 mem = 536879137 peak = 898179072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280155, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 388, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (396.65, 174.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674298 sequences (100000270 bp) in 2.535 CPU sec, 2.592 real sec...
[M::mem_process_seqs] Processed 674368 reads in 93.954 CPU sec, 3.006 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280248, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 386, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (394.69, 172.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 290, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (51, 466)
[M::mem_pestat] mean and std.dev: (236.56, 70.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 549)
[M::mem_pestat] skip orientation RR
[M::process] read 674534 sequences (100000199 bp) in 3.105 CPU sec, 3.276 real sec...
[M::mem_process_seqs] Processed 674298 reads in 92.696 CPU sec, 2.989 real sec
sorting block 29, #reads = 1065574 mem = 536880973 peak = 898179072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279885, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 383, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (392.48, 172.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674534 reads in 90.587 CPU sec, 2.907 real sec
[M::process] read 674800 sequences (100000115 bp) in 3.122 CPU sec, 3.270 real sec...
sorting block 30, #reads = 1065345 mem = 536879021 peak = 898179072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280200, 7, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 381, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (390.14, 171.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 413, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (319.60, 94.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 881)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 674800 reads in 90.934 CPU sec, 2.929 real sec
[M::process] read 674666 sequences (100000007 bp) in 3.742 CPU sec, 3.930 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280614, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 379, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (388.81, 171.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674898 sequences (100000121 bp) in 2.790 CPU sec, 2.887 real sec...
[M::mem_process_seqs] Processed 674666 reads in 93.749 CPU sec, 3.008 real sec
sorting block 31, #reads = 1065735 mem = 536876973 peak = 898179072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280240, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 380, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (389.40, 172.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674898 reads in 94.804 CPU sec, 3.047 real sec
[M::process] read 676176 sequences (100000102 bp) in 3.607 CPU sec, 3.804 real sec...
sorting block 32, #reads = 1064088 mem = 536874778 peak = 898179072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281103, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 368, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (377.07, 166.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674568 sequences (100000249 bp) in 2.751 CPU sec, 2.847 real sec...
[M::mem_process_seqs] Processed 676176 reads in 98.031 CPU sec, 3.132 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280055, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 380, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (390.71, 172.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674568 reads in 93.355 CPU sec, 3.009 real sec
[M::process] read 674364 sequences (100000123 bp) in 3.643 CPU sec, 3.826 real sec...
sorting block 33, #reads = 1062450 mem = 536878830 peak = 898179072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280325, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 380, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (390.85, 172.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674566 sequences (100000036 bp) in 2.853 CPU sec, 2.977 real sec...
[M::mem_process_seqs] Processed 674364 reads in 92.666 CPU sec, 2.985 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280317, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 378, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (389.46, 171.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 34, #reads = 1064703 mem = 536886209 peak = 898179072
[M::mem_process_seqs] Processed 674566 reads in 90.670 CPU sec, 2.929 real sec
[M::process] read 674380 sequences (100000023 bp) in 3.649 CPU sec, 3.870 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280553, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 378, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (389.22, 171.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674350 sequences (100000287 bp) in 2.419 CPU sec, 2.500 real sec...
[M::mem_process_seqs] Processed 674380 reads in 89.028 CPU sec, 2.844 real sec
sorting block 35, #reads = 1065408 mem = 536880100 peak = 898179072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280378, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 380, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (390.10, 170.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674350 reads in 90.302 CPU sec, 2.899 real sec
[M::process] read 674228 sequences (100000190 bp) in 3.682 CPU sec, 3.866 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281018, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 380, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (390.74, 170.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674228 reads in 89.232 CPU sec, 2.873 real sec
sorting block 36, #reads = 1064497 mem = 536878780 peak = 898179072
[M::process] read 674084 sequences (100000237 bp) in 3.266 CPU sec, 3.414 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280772, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 380, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (390.60, 170.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674084 reads in 90.098 CPU sec, 2.899 real sec
[M::process] read 674046 sequences (100000264 bp) in 3.321 CPU sec, 3.481 real sec...
sorting block 37, #reads = 1064682 mem = 536878218 peak = 898179072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280310, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 383, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (393.23, 172.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673704 sequences (100000268 bp) in 2.322 CPU sec, 2.462 real sec...
[M::mem_process_seqs] Processed 674046 reads in 87.839 CPU sec, 2.820 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280216, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 387, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (397.73, 174.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673704 reads in 86.974 CPU sec, 2.790 real sec
[M::process] read 674180 sequences (100000097 bp) in 3.264 CPU sec, 3.441 real sec...
sorting block 38, #reads = 1063862 mem = 536878203 peak = 898179072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281030, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 388, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (398.20, 176.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674180 reads in 86.024 CPU sec, 2.765 real sec
[M::process] read 675684 sequences (100000256 bp) in 3.324 CPU sec, 3.500 real sec...
sorting block 39, #reads = 1063816 mem = 536880667 peak = 898179072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281209, 11, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 380, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (388.35, 173.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 533, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1595)
[M::mem_pestat] mean and std.dev: (297.00, 263.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2126)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 375, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1136)
[M::mem_pestat] mean and std.dev: (347.50, 187.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1448)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675684 reads in 86.406 CPU sec, 2.783 real sec
[M::process] read 675340 sequences (100000162 bp) in 3.137 CPU sec, 3.275 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281372, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 382, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (391.60, 173.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675340 reads in 86.620 CPU sec, 2.784 real sec
[M::process] read 674708 sequences (100000291 bp) in 3.252 CPU sec, 3.428 real sec...
sorting block 40, #reads = 1064536 mem = 536877963 peak = 898179072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280825, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 388, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (396.40, 174.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674708 reads in 86.080 CPU sec, 2.775 real sec
[M::process] read 674322 sequences (100000168 bp) in 3.009 CPU sec, 3.135 real sec...
sorting block 41, #reads = 1064868 mem = 536880422 peak = 898179072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280825, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 390, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (397.19, 173.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674322 reads in 84.359 CPU sec, 2.732 real sec
[M::process] read 674058 sequences (100000014 bp) in 3.401 CPU sec, 3.639 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 280163, 2, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 343, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (229.64, 95.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 395, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (401.68, 174.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 674058 reads in 83.747 CPU sec, 2.691 real sec
[M::process] read 673714 sequences (100000098 bp) in 2.618 CPU sec, 2.756 real sec...
sorting block 42, #reads = 1064423 mem = 536880124 peak = 898179072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279997, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 396, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (403.30, 174.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673714 reads in 85.634 CPU sec, 2.765 real sec
[M::process] read 673672 sequences (100000079 bp) in 3.674 CPU sec, 3.916 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280635, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 399, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (405.56, 175.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1291)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673450 sequences (100000125 bp) in 2.346 CPU sec, 2.431 real sec...
[M::mem_process_seqs] Processed 673672 reads in 83.650 CPU sec, 2.671 real sec
sorting block 43, #reads = 1064521 mem = 536879287 peak = 898179072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280774, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 403, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (407.55, 175.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673450 reads in 83.130 CPU sec, 2.688 real sec
[M::process] read 673236 sequences (100000068 bp) in 3.683 CPU sec, 3.912 real sec...
sorting block 44, #reads = 1064894 mem = 536878357 peak = 898179072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280378, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (275, 407, 535)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055)
[M::mem_pestat] mean and std.dev: (412.39, 178.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1315)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673230 sequences (100000234 bp) in 2.560 CPU sec, 2.661 real sec...
[M::mem_process_seqs] Processed 673236 reads in 83.127 CPU sec, 2.661 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280311, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (277, 408, 537)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1057)
[M::mem_pestat] mean and std.dev: (413.76, 178.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1317)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673230 reads in 85.266 CPU sec, 2.761 real sec
sorting block 45, #reads = 1064570 mem = 536877269 peak = 898179072
[M::process] read 673632 sequences (100000104 bp) in 3.917 CPU sec, 4.180 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280008, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 402, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054)
[M::mem_pestat] mean and std.dev: (408.61, 177.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1315)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674584 sequences (100000245 bp) in 2.346 CPU sec, 2.380 real sec...
[M::mem_process_seqs] Processed 673632 reads in 84.083 CPU sec, 2.681 real sec
sorting block 46, #reads = 1064149 mem = 536889151 peak = 898179072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280557, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 390, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (398.67, 176.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1297)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674584 reads in 86.848 CPU sec, 2.804 real sec
[M::process] read 674262 sequences (100000137 bp) in 3.878 CPU sec, 4.074 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280636, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 390, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (398.59, 175.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (125, 233, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (205.00, 79.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 674262 reads in 85.156 CPU sec, 2.726 real sec
[M::process] read 674034 sequences (100000085 bp) in 2.625 CPU sec, 2.746 real sec...
sorting block 47, #reads = 1065917 mem = 536878909 peak = 898179072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 280558, 1, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 415, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052)
[M::mem_pestat] mean and std.dev: (321.19, 146.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1346)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 392, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (399.94, 175.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 674034 reads in 86.984 CPU sec, 2.805 real sec
[M::process] read 674080 sequences (100000151 bp) in 2.822 CPU sec, 3.066 real sec...
sorting block 48, #reads = 1066089 mem = 536878384 peak = 898179072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280590, 4, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 390, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037)
[M::mem_pestat] mean and std.dev: (399.60, 176.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (323, 898, 1330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3344)
[M::mem_pestat] mean and std.dev: (842.85, 486.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4351)
[M::mem_pestat] skip orientation RR
[M::process] read 673998 sequences (100000180 bp) in 2.535 CPU sec, 2.747 real sec...
[M::mem_process_seqs] Processed 674080 reads in 87.626 CPU sec, 2.821 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280468, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 388, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (397.70, 175.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673998 reads in 89.018 CPU sec, 2.875 real sec
[M::process] read 674186 sequences (100000220 bp) in 3.709 CPU sec, 3.910 real sec...
sorting block 49, #reads = 1064670 mem = 536880265 peak = 898179072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280405, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 388, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (398.16, 175.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674186 reads in 84.362 CPU sec, 2.706 real sec
[M::process] read 674342 sequences (100000107 bp) in 2.647 CPU sec, 2.735 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280240, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 385, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (395.64, 175.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674342 reads in 86.867 CPU sec, 2.799 real sec
sorting block 50, #reads = 1064697 mem = 536880847 peak = 898179072
[M::process] read 674362 sequences (100000051 bp) in 3.569 CPU sec, 3.816 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280586, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 385, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (395.22, 174.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674400 sequences (100000292 bp) in 2.629 CPU sec, 2.751 real sec...
[M::mem_process_seqs] Processed 674362 reads in 87.548 CPU sec, 2.811 real sec
sorting block 51, #reads = 1064235 mem = 536877534 peak = 898179072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279763, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 383, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (393.21, 174.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674400 reads in 86.350 CPU sec, 2.786 real sec
[M::process] read 675034 sequences (100000144 bp) in 3.829 CPU sec, 4.087 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281283, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 377, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (387.88, 172.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674594 sequences (100000119 bp) in 2.393 CPU sec, 2.507 real sec...
[M::mem_process_seqs] Processed 675034 reads in 87.189 CPU sec, 2.786 real sec
sorting block 52, #reads = 1065192 mem = 536878033 peak = 916877312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281483, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 384, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (393.43, 173.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674594 reads in 87.740 CPU sec, 2.824 real sec
[M::process] read 674278 sequences (100000043 bp) in 4.258 CPU sec, 4.564 real sec...
sorting block 53, #reads = 1063898 mem = 536881631 peak = 916877312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280663, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 386, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (395.93, 174.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674238 sequences (100000157 bp) in 2.528 CPU sec, 2.667 real sec...
[M::mem_process_seqs] Processed 674278 reads in 85.919 CPU sec, 2.753 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280793, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 387, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (397.90, 176.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674238 reads in 85.629 CPU sec, 2.756 real sec
sorting block 54, #reads = 1064933 mem = 536879582 peak = 916877312
[M::process] read 674176 sequences (100000032 bp) in 3.957 CPU sec, 4.244 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280183, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 386, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (396.77, 175.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674208 sequences (100000129 bp) in 2.256 CPU sec, 2.347 real sec...
[M::mem_process_seqs] Processed 674176 reads in 82.550 CPU sec, 2.646 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280095, 3, 18)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 386, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (396.48, 176.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 294, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1078)
[M::mem_pestat] mean and std.dev: (336.44, 141.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1370)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 674208 reads in 85.337 CPU sec, 2.752 real sec
sorting block 55, #reads = 1065152 mem = 536880725 peak = 916877312
[M::process] read 674266 sequences (100000050 bp) in 3.009 CPU sec, 3.139 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280801, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 386, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (396.70, 175.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674096 sequences (100000006 bp) in 2.177 CPU sec, 2.331 real sec...
[M::mem_process_seqs] Processed 674266 reads in 83.379 CPU sec, 2.676 real sec
sorting block 56, #reads = 1065071 mem = 536879015 peak = 916877312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280686, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 387, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (397.63, 176.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674096 reads in 84.930 CPU sec, 2.726 real sec
[M::process] read 673966 sequences (100000022 bp) in 2.993 CPU sec, 3.087 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280765, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 387, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (397.69, 176.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673966 reads in 84.419 CPU sec, 2.714 real sec
sorting block 57, #reads = 1064322 mem = 536883400 peak = 916877312
[M::process] read 673912 sequences (100000040 bp) in 3.084 CPU sec, 3.262 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 280455, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 388, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (398.84, 177.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673684 sequences (100000277 bp) in 2.398 CPU sec, 2.491 real sec...
[M::mem_process_seqs] Processed 673912 reads in 82.283 CPU sec, 2.647 real sec
sorting block 58, #reads = 1064612 mem = 536877794 peak = 916877312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280175, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 391, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (401.97, 178.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673684 reads in 82.430 CPU sec, 2.648 real sec
[M::process] read 674060 sequences (100000220 bp) in 3.199 CPU sec, 3.345 real sec...
[M::process] read 675042 sequences (100000291 bp) in 2.103 CPU sec, 2.207 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280836, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 387, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (397.33, 175.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674728 sequences (100000090 bp) in 1.850 CPU sec, 1.857 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280383, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 382, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (391.15, 173.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674060 reads in 157.613 CPU sec, 4.948 real sec
[M::mem_process_seqs] Processed 675042 reads in 103.684 CPU sec, 3.253 real sec
sorting block 59, #reads = 1064396 mem = 536880030 peak = 916877312
[M::process] read 674324 sequences (100000289 bp) in 2.658 CPU sec, 2.750 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280096, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 387, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (395.82, 174.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 60, #reads = 1064410 mem = 536879555 peak = 916877312
[M::process] read 674088 sequences (100000109 bp) in 2.768 CPU sec, 2.899 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280120, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 392, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040)
[M::mem_pestat] mean and std.dev: (400.27, 176.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674728 reads in 155.054 CPU sec, 4.937 real sec
[M::mem_process_seqs] Processed 674324 reads in 101.269 CPU sec, 3.195 real sec
sorting block 61, #reads = 1065582 mem = 536879296 peak = 916877312
[M::process] read 673816 sequences (100000285 bp) in 2.277 CPU sec, 2.343 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 279971, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 396, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046)
[M::mem_pestat] mean and std.dev: (403.45, 177.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1306)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673598 sequences (100000003 bp) in 2.215 CPU sec, 2.270 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280147, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 398, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052)
[M::mem_pestat] mean and std.dev: (406.11, 178.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1313)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674088 reads in 156.038 CPU sec, 4.922 real sec
[M::mem_process_seqs] Processed 673816 reads in 99.143 CPU sec, 3.108 real sec
[M::process] read 673462 sequences (100000301 bp) in 1.902 CPU sec, 1.936 real sec...
sorting block 62, #reads = 1065651 mem = 536880260 peak = 916877312
[M::process] read 673342 sequences (100000031 bp) in 2.219 CPU sec, 2.292 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 279499, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 400, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052)
[M::mem_pestat] mean and std.dev: (407.39, 178.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1313)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672954 sequences (100000093 bp) in 1.550 CPU sec, 1.554 real sec...
[M::mem_process_seqs] Processed 673462 reads in 82.747 CPU sec, 2.605 real sec
sorting block 63, #reads = 1065463 mem = 536881387 peak = 921206784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 279572, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 401, 531)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051)
[M::mem_pestat] mean and std.dev: (408.00, 177.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1311)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279631, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (276, 406, 535)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053)
[M::mem_pestat] mean and std.dev: (411.80, 177.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1312)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672910 sequences (100000004 bp) in 1.879 CPU sec, 1.888 real sec...
[M::mem_process_seqs] Processed 673598 reads in 233.124 CPU sec, 7.381 real sec
[M::mem_process_seqs] Processed 673342 reads in 113.289 CPU sec, 3.601 real sec
sorting block 64, #reads = 1065570 mem = 536880435 peak = 971829248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 279742, 3, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (1122, 1701, 1701)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2859)
[M::mem_pestat] mean and std.dev: (1344.57, 601.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3751)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (277, 407, 534)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048)
[M::mem_pestat] mean and std.dev: (411.98, 176.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1305)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 672948 sequences (100000164 bp) in 2.629 CPU sec, 2.732 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 279475, 1, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 226, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490)
[M::mem_pestat] mean and std.dev: (197.92, 63.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 604)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (278, 410, 539)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1061)
[M::mem_pestat] mean and std.dev: (414.87, 178.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1322)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674152 sequences (100000201 bp) in 1.640 CPU sec, 1.645 real sec...
[M::mem_process_seqs] Processed 672954 reads in 150.668 CPU sec, 4.760 real sec
[M::mem_process_seqs] Processed 672910 reads in 98.493 CPU sec, 3.106 real sec
sorting block 65, #reads = 1065897 mem = 536882753 peak = 972595200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279076, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (277, 409, 538)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060)
[M::mem_pestat] mean and std.dev: (414.48, 178.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1321)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674274 sequences (100000283 bp) in 2.058 CPU sec, 2.141 real sec...
[M::process] read 673912 sequences (100000046 bp) in 1.650 CPU sec, 1.650 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279829, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 396, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051)
[M::mem_pestat] mean and std.dev: (403.59, 177.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1313)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672948 reads in 157.026 CPU sec, 4.980 real sec
sorting block 66, #reads = 1064139 mem = 536881087 peak = 972595200
[M::mem_process_seqs] Processed 674152 reads in 101.515 CPU sec, 3.216 real sec
sorting block 67, #reads = 1065644 mem = 536881358 peak = 1079775232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280197, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 394, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052)
[M::mem_pestat] mean and std.dev: (402.26, 178.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1315)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674056 sequences (100000130 bp) in 2.493 CPU sec, 2.574 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280153, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 395, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054)
[M::mem_pestat] mean and std.dev: (403.64, 177.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1317)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674282 sequences (100000205 bp) in 1.830 CPU sec, 1.835 real sec...
[M::mem_process_seqs] Processed 674274 reads in 156.280 CPU sec, 4.940 real sec
[M::mem_process_seqs] Processed 673912 reads in 101.048 CPU sec, 3.196 real sec
sorting block 68, #reads = 1066137 mem = 536879188 peak = 1079775232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280084, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 391, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (399.73, 175.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1296)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674344 sequences (100000020 bp) in 2.560 CPU sec, 2.631 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279790, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 674304 sequences (100000149 bp) in 1.730 CPU sec, 1.730 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (261, 388, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (397.17, 174.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674056 reads in 157.576 CPU sec, 4.980 real sec
[M::mem_process_seqs] Processed 674282 reads in 102.188 CPU sec, 3.230 real sec
sorting block 69, #reads = 1064648 mem = 536879640 peak = 1079775232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 279457, 1, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 514, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1218)
[M::mem_pestat] mean and std.dev: (347.44, 233.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1570)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 386, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (395.30, 174.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 163, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (236.93, 131.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 674370 sequences (100000083 bp) in 2.521 CPU sec, 2.602 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280455, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 386, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (394.95, 173.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (280, 747, 747)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1681)
[M::mem_pestat] mean and std.dev: (648.00, 438.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2401)
[M::mem_pestat] skip orientation RR
[M::process] read 674310 sequences (100000082 bp) in 1.760 CPU sec, 1.755 real sec...
[M::mem_process_seqs] Processed 674344 reads in 159.966 CPU sec, 5.060 real sec
[M::mem_process_seqs] Processed 674304 reads in 102.885 CPU sec, 3.255 real sec
sorting block 70, #reads = 1064746 mem = 536881382 peak = 1079775232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280408, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 385, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (393.15, 172.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674190 sequences (100000157 bp) in 2.879 CPU sec, 2.941 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 279947, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 383, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (392.49, 172.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674370 reads in 161.624 CPU sec, 5.102 real sec
sorting block 71, #reads = 1064912 mem = 536884786 peak = 1079775232
[M::process] read 674308 sequences (100000225 bp) in 2.425 CPU sec, 2.440 real sec...
[M::mem_process_seqs] Processed 674310 reads in 105.268 CPU sec, 3.306 real sec
sorting block 72, #reads = 1065036 mem = 536879952 peak = 1079775232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280403, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 385, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (393.26, 171.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674202 sequences (100000112 bp) in 2.767 CPU sec, 2.846 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280280, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 385, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (394.50, 172.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674190 reads in 160.427 CPU sec, 5.074 real sec
[M::mem_process_seqs] Processed 674308 reads in 101.591 CPU sec, 3.182 real sec
[M::process] read 674314 sequences (100000051 bp) in 2.361 CPU sec, 2.377 real sec...
sorting block 73, #reads = 1064725 mem = 536879769 peak = 1079775232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 280521, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 386, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (395.63, 174.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674454 sequences (100000197 bp) in 2.848 CPU sec, 2.922 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280663, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 386, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (395.17, 174.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674202 reads in 153.797 CPU sec, 4.871 real sec
[M::mem_process_seqs] Processed 674314 reads in 99.063 CPU sec, 3.105 real sec
[M::process] read 674412 sequences (100000159 bp) in 2.541 CPU sec, 2.576 real sec...
sorting block 74, #reads = 1064679 mem = 536878149 peak = 1079775232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279640, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 386, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (395.72, 175.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1291)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674086 sequences (100000071 bp) in 2.548 CPU sec, 2.628 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280402, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 383, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (392.95, 172.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674454 reads in 154.757 CPU sec, 4.901 real sec
[M::mem_process_seqs] Processed 674412 reads in 98.742 CPU sec, 3.103 real sec
sorting block 75, #reads = 1064605 mem = 536878407 peak = 1079775232
[M::process] read 674038 sequences (100000157 bp) in 2.358 CPU sec, 2.392 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280343, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 386, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (395.16, 172.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673758 sequences (100000066 bp) in 2.758 CPU sec, 2.821 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280463, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 387, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (395.93, 172.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674086 reads in 149.609 CPU sec, 4.739 real sec
sorting block 76, #reads = 1064929 mem = 536879743 peak = 1079775232
[M::mem_process_seqs] Processed 674038 reads in 95.982 CPU sec, 3.011 real sec
[M::process] read 673858 sequences (100000251 bp) in 2.290 CPU sec, 2.360 real sec...
sorting block 77, #reads = 1064353 mem = 536877207 peak = 1079775232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280755, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 390, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (398.13, 173.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673736 sequences (100000185 bp) in 2.658 CPU sec, 2.727 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280366, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 390, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (399.18, 174.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673758 reads in 146.935 CPU sec, 4.647 real sec
[M::mem_process_seqs] Processed 673858 reads in 95.151 CPU sec, 2.980 real sec
[M::process] read 673620 sequences (100000040 bp) in 2.496 CPU sec, 2.552 real sec...
sorting block 78, #reads = 1064213 mem = 536878399 peak = 1079775232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280760, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 391, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (400.36, 174.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677802 sequences (100000081 bp) in 2.676 CPU sec, 2.744 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281122, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 395, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (403.65, 176.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673736 reads in 146.697 CPU sec, 4.663 real sec
[M::mem_process_seqs] Processed 673620 reads in 96.294 CPU sec, 3.024 real sec
sorting block 79, #reads = 1064174 mem = 536878706 peak = 1079775232
[M::process] read 677484 sequences (100000106 bp) in 2.802 CPU sec, 2.855 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281872, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 364, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (376.02, 173.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1296)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677272 sequences (100000165 bp) in 2.565 CPU sec, 2.612 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282131, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 369, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045)
[M::mem_pestat] mean and std.dev: (380.30, 175.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1314)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677802 reads in 163.841 CPU sec, 5.185 real sec
[M::mem_process_seqs] Processed 677484 reads in 105.254 CPU sec, 3.305 real sec
sorting block 80, #reads = 1064079 mem = 536882592 peak = 1079775232
[M::process] read 677248 sequences (100000177 bp) in 2.660 CPU sec, 2.706 real sec...
sorting block 81, #reads = 1063547 mem = 536880430 peak = 1079775232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281392, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 373, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050)
[M::mem_pestat] mean and std.dev: (382.90, 175.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1320)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676790 sequences (100000242 bp) in 2.667 CPU sec, 2.723 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 281713, 0, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (288, 590, 590)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1194)
[M::mem_pestat] mean and std.dev: (463.80, 303.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1678)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 370, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (378.80, 171.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 677272 reads in 171.436 CPU sec, 5.423 real sec
[M::process] read 676156 sequences (100000171 bp) in 2.016 CPU sec, 2.040 real sec...
[M::mem_process_seqs] Processed 677248 reads in 111.616 CPU sec, 3.505 real sec
sorting block 82, #reads = 1062363 mem = 536879362 peak = 1079775232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281929, 7, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 376, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (382.56, 170.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (110, 150, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (189.60, 107.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RR
[M::process] read 675530 sequences (100000214 bp) in 2.649 CPU sec, 2.728 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280858, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 381, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (387.20, 172.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1297)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676790 reads in 167.337 CPU sec, 5.295 real sec
[M::process] read 675388 sequences (100000003 bp) in 2.211 CPU sec, 2.223 real sec...
[M::mem_process_seqs] Processed 676156 reads in 107.636 CPU sec, 3.381 real sec
sorting block 83, #reads = 1061413 mem = 536880168 peak = 1079775232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 281597, 0, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (387, 387, 1258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3000)
[M::mem_pestat] mean and std.dev: (657.73, 426.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3871)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 385, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (390.33, 171.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675498 sequences (100000136 bp) in 2.894 CPU sec, 2.956 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280426, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 387, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (392.28, 172.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1292)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675530 reads in 164.278 CPU sec, 5.192 real sec
[M::mem_process_seqs] Processed 675388 reads in 104.609 CPU sec, 3.281 real sec
[M::process] read 676822 sequences (100000103 bp) in 2.377 CPU sec, 2.383 real sec...
sorting block 84, #reads = 1060986 mem = 536881485 peak = 1079775232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281196, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 386, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (390.98, 171.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677468 sequences (100000012 bp) in 2.563 CPU sec, 2.636 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282144, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 372, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (378.50, 169.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675498 reads in 162.859 CPU sec, 5.154 real sec
sorting block 85, #reads = 1061041 mem = 536880729 peak = 1079775232
[M::mem_process_seqs] Processed 676822 reads in 105.890 CPU sec, 3.323 real sec
[M::process] read 677400 sequences (100000262 bp) in 2.519 CPU sec, 2.538 real sec...
sorting block 86, #reads = 1061786 mem = 536879339 peak = 1087655936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282538, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 368, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (375.46, 169.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677348 sequences (100000208 bp) in 2.465 CPU sec, 2.544 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282846, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 365, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (373.60, 168.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677818 sequences (100000189 bp) in 1.940 CPU sec, 1.942 real sec...
[M::mem_process_seqs] Processed 677468 reads in 167.309 CPU sec, 5.298 real sec
[M::mem_process_seqs] Processed 677400 reads in 107.877 CPU sec, 3.400 real sec
sorting block 87, #reads = 1062540 mem = 536880746 peak = 1087655936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282467, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 366, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (374.13, 167.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676594 sequences (100000047 bp) in 2.779 CPU sec, 2.842 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282213, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 364, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (372.25, 166.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677348 reads in 176.311 CPU sec, 5.569 real sec
[M::process] read 675970 sequences (100000163 bp) in 2.290 CPU sec, 2.291 real sec...
[M::mem_process_seqs] Processed 677818 reads in 117.000 CPU sec, 3.674 real sec
sorting block 88, #reads = 1059896 mem = 536879778 peak = 1087655936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281399, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 372, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (379.58, 168.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676178 sequences (100000149 bp) in 2.460 CPU sec, 2.534 real sec...
[M::process] read 679100 sequences (100000138 bp) in 1.840 CPU sec, 1.843 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281384, 2, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 375, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (382.05, 168.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 418, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (420.85, 220.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1303)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 676594 reads in 165.376 CPU sec, 5.234 real sec
sorting block 89, #reads = 1058797 mem = 536880468 peak = 1087655936
[M::mem_process_seqs] Processed 675970 reads in 105.821 CPU sec, 3.355 real sec
sorting block 90, #reads = 1060953 mem = 536877460 peak = 1087655936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281650, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 377, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (383.20, 169.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677232 sequences (100000061 bp) in 2.227 CPU sec, 2.303 real sec...
[M::process] read 676952 sequences (100000024 bp) in 1.820 CPU sec, 1.815 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283140, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 349, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (357.76, 160.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676178 reads in 174.305 CPU sec, 5.533 real sec
[M::mem_process_seqs] Processed 679100 reads in 119.095 CPU sec, 3.773 real sec
sorting block 91, #reads = 1060463 mem = 536880788 peak = 1087655936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282420, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 364, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (372.90, 165.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676024 sequences (100000250 bp) in 3.105 CPU sec, 3.160 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282437, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 676146 sequences (100000153 bp) in 1.880 CPU sec, 1.882 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (240, 362, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (369.44, 162.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677232 reads in 178.435 CPU sec, 5.631 real sec
[M::mem_process_seqs] Processed 676952 reads in 116.708 CPU sec, 3.688 real sec
sorting block 92, #reads = 1056318 mem = 536880785 peak = 1087655936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281702, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 373, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (380.52, 167.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675718 sequences (100000237 bp) in 2.401 CPU sec, 2.467 real sec...
[M::process] read 675950 sequences (100000023 bp) in 1.780 CPU sec, 1.780 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 282200, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 372, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (379.26, 167.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676024 reads in 170.534 CPU sec, 5.401 real sec
[M::mem_process_seqs] Processed 676146 reads in 110.670 CPU sec, 3.503 real sec
sorting block 93, #reads = 1058348 mem = 536879903 peak = 1087655936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281718, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 377, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (384.55, 169.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676324 sequences (100000180 bp) in 2.823 CPU sec, 2.872 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282000, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 377, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (384.49, 170.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675718 reads in 168.342 CPU sec, 5.315 real sec
sorting block 94, #reads = 1058574 mem = 536879549 peak = 1087655936
[M::process] read 675808 sequences (100000229 bp) in 2.472 CPU sec, 2.483 real sec...
[M::mem_process_seqs] Processed 675950 reads in 108.085 CPU sec, 3.383 real sec
sorting block 95, #reads = 1058641 mem = 536881052 peak = 1108467712
[M::process] read 680264 sequences (100000208 bp) in 2.835 CPU sec, 2.894 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282066, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 379, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (388.69, 174.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1302)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675808 reads in 98.144 CPU sec, 3.063 real sec
[M::process] read 680068 sequences (100000015 bp) in 2.431 CPU sec, 2.446 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282080, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 371, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (380.18, 170.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (303, 313, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (13, 738)
[M::mem_pestat] mean and std.dev: (332.91, 141.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283240, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 343, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (357.36, 167.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676324 reads in 277.524 CPU sec, 8.758 real sec
[M::process] read 678220 sequences (100000153 bp) in 2.611 CPU sec, 2.617 real sec...
sorting block 96, #reads = 1058364 mem = 536879920 peak = 1108467712
[M::mem_process_seqs] Processed 680264 reads in 139.621 CPU sec, 4.400 real sec
sorting block 97, #reads = 1056677 mem = 536884234 peak = 1108467712
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 283743, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 343, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (355.64, 164.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678260 sequences (100000154 bp) in 3.192 CPU sec, 3.285 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282412, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 358, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (367.92, 166.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680068 reads in 176.153 CPU sec, 5.550 real sec
[M::process] read 677376 sequences (100000187 bp) in 2.336 CPU sec, 2.339 real sec...
[M::mem_process_seqs] Processed 678220 reads in 113.040 CPU sec, 3.554 real sec
sorting block 98, #reads = 1057636 mem = 536878094 peak = 1108467712
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282660, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 360, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (368.46, 166.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676776 sequences (100000178 bp) in 2.996 CPU sec, 3.075 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282596, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 366, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (373.11, 166.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 417, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (319.44, 117.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 678260 reads in 169.989 CPU sec, 5.363 real sec
[M::process] read 676250 sequences (100000253 bp) in 2.558 CPU sec, 2.568 real sec...
sorting block 99, #reads = 1059179 mem = 536879227 peak = 1108467712
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282775, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 372, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (378.23, 167.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676196 sequences (100000136 bp) in 2.325 CPU sec, 2.331 real sec...
[M::mem_process_seqs] Processed 676776 reads in 93.918 CPU sec, 2.958 real sec
[M::mem_process_seqs] Processed 677376 reads in 226.298 CPU sec, 7.126 real sec
sorting block 100, #reads = 1057620 mem = 536881476 peak = 1108467712
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282096, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 375, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (381.23, 167.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677092 sequences (100000172 bp) in 3.058 CPU sec, 3.137 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281799, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 377, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (383.52, 169.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676250 reads in 182.473 CPU sec, 5.759 real sec
[M::process] read 677018 sequences (100000116 bp) in 2.584 CPU sec, 2.614 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 282553, 0, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (111, 204, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (232.00, 119.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 367, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (375.95, 168.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 297, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509)
[M::mem_pestat] mean and std.dev: (213.30, 76.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 615)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 677092 reads in 93.043 CPU sec, 2.916 real sec
sorting block 101, #reads = 1059231 mem = 536878394 peak = 1108467712
[M::process] read 676794 sequences (100000186 bp) in 2.308 CPU sec, 2.313 real sec...
[M::mem_process_seqs] Processed 676196 reads in 225.631 CPU sec, 7.096 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281421, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 369, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (376.71, 169.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 102, #reads = 1061195 mem = 536880589 peak = 1139998720
[M::process] read 676760 sequences (100000218 bp) in 2.964 CPU sec, 3.033 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281660, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 367, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (376.64, 169.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677018 reads in 180.833 CPU sec, 5.704 real sec
[M::mem_process_seqs] Processed 676794 reads in 107.589 CPU sec, 3.376 real sec
[M::process] read 676972 sequences (100000011 bp) in 2.037 CPU sec, 2.076 real sec...
sorting block 103, #reads = 1061568 mem = 536879396 peak = 1139998720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281688, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 370, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (378.89, 170.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677222 sequences (100000017 bp) in 2.648 CPU sec, 2.712 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282433, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 369, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (377.85, 169.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676664 sequences (100000269 bp) in 1.770 CPU sec, 1.763 real sec...
[M::mem_process_seqs] Processed 676760 reads in 167.520 CPU sec, 5.305 real sec
sorting block 104, #reads = 1061362 mem = 536887223 peak = 1139998720
[M::mem_process_seqs] Processed 676972 reads in 110.401 CPU sec, 3.472 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 282547, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 367, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (374.98, 168.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678204 sequences (100000185 bp) in 3.034 CPU sec, 3.102 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282283, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 368, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (377.11, 168.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676926 sequences (100000120 bp) in 2.310 CPU sec, 2.311 real sec...
[M::mem_process_seqs] Processed 677222 reads in 175.920 CPU sec, 5.538 real sec
sorting block 105, #reads = 1059719 mem = 536878544 peak = 1139998720
[M::mem_process_seqs] Processed 676664 reads in 115.074 CPU sec, 3.621 real sec
sorting block 106, #reads = 1058771 mem = 536877486 peak = 1139998720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282885, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 357, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (366.92, 166.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675334 sequences (100000262 bp) in 2.757 CPU sec, 2.817 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281802, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 366, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (376.24, 169.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675202 sequences (100000062 bp) in 2.300 CPU sec, 2.300 real sec...
[M::mem_process_seqs] Processed 678204 reads in 186.759 CPU sec, 5.901 real sec
[M::mem_process_seqs] Processed 676926 reads in 120.476 CPU sec, 3.801 real sec
sorting block 107, #reads = 1056282 mem = 536880764 peak = 1139998720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281381, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 384, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048)
[M::mem_pestat] mean and std.dev: (392.85, 175.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1313)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675116 sequences (100000116 bp) in 3.024 CPU sec, 3.097 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 281806, 0, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (472, 529, 561)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (294, 739)
[M::mem_pestat] mean and std.dev: (525.25, 34.64)
[M::mem_pestat] low and high boundaries for proper pairs: (205, 828)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 383, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1049)
[M::mem_pestat] mean and std.dev: (391.45, 175.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1315)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675334 reads in 166.944 CPU sec, 5.267 real sec
[M::mem_process_seqs] Processed 675202 reads in 108.446 CPU sec, 3.395 real sec
[M::process] read 674768 sequences (100000140 bp) in 2.507 CPU sec, 2.521 real sec...
sorting block 108, #reads = 1060556 mem = 536879178 peak = 1139998720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 280844, 4, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 182, 703)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1815)
[M::mem_pestat] mean and std.dev: (373.40, 274.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2371)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 386, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1061)
[M::mem_pestat] mean and std.dev: (395.16, 177.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1330)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674842 sequences (100000001 bp) in 2.281 CPU sec, 2.355 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281566, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 391, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1069)
[M::mem_pestat] mean and std.dev: (399.32, 179.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1340)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674612 sequences (100000222 bp) in 1.730 CPU sec, 1.735 real sec...
[M::mem_process_seqs] Processed 675116 reads in 167.157 CPU sec, 5.307 real sec
sorting block 109, #reads = 1061539 mem = 536881501 peak = 1139998720
[M::mem_process_seqs] Processed 674768 reads in 107.535 CPU sec, 3.396 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281650, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 394, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1080)
[M::mem_pestat] mean and std.dev: (402.75, 181.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1354)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674720 sequences (100000101 bp) in 2.853 CPU sec, 2.921 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280516, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 396, 534)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1084)
[M::mem_pestat] mean and std.dev: (404.27, 182.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1359)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 505, 966)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2472)
[M::mem_pestat] mean and std.dev: (541.00, 374.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3225)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 674842 reads in 160.786 CPU sec, 5.072 real sec
sorting block 110, #reads = 1061484 mem = 536879864 peak = 1139998720
[M::mem_process_seqs] Processed 674612 reads in 102.488 CPU sec, 3.212 real sec
[M::process] read 674632 sequences (100000080 bp) in 2.428 CPU sec, 2.451 real sec...
sorting block 111, #reads = 1061307 mem = 536882117 peak = 1139998720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281136, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 394, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1081)
[M::mem_pestat] mean and std.dev: (404.17, 182.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1355)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676050 sequences (100000001 bp) in 2.792 CPU sec, 2.852 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280495, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 394, 535)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1087)
[M::mem_pestat] mean and std.dev: (405.78, 185.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1363)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674720 reads in 165.047 CPU sec, 5.226 real sec
[M::mem_process_seqs] Processed 674632 reads in 108.728 CPU sec, 3.408 real sec
[M::process] read 677226 sequences (100000039 bp) in 2.540 CPU sec, 2.559 real sec...
sorting block 112, #reads = 1061132 mem = 536878069 peak = 1139998720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281796, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 379, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060)
[M::mem_pestat] mean and std.dev: (391.58, 180.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1331)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675732 sequences (100000046 bp) in 2.829 CPU sec, 2.903 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281971, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 373, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1065)
[M::mem_pestat] mean and std.dev: (386.34, 181.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1340)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676050 reads in 179.686 CPU sec, 5.678 real sec
[M::process] read 675496 sequences (100000033 bp) in 2.238 CPU sec, 2.238 real sec...
[M::mem_process_seqs] Processed 677226 reads in 109.259 CPU sec, 3.432 real sec
sorting block 113, #reads = 1053660 mem = 536878076 peak = 1139998720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281287, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 387, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075)
[M::mem_pestat] mean and std.dev: (396.63, 182.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1350)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675620 sequences (100000235 bp) in 2.718 CPU sec, 2.790 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 281587, 2, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (256, 323, 904)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2200)
[M::mem_pestat] mean and std.dev: (411.77, 277.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2848)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 389, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1071)
[M::mem_pestat] mean and std.dev: (397.40, 180.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1344)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675732 reads in 157.144 CPU sec, 4.972 real sec
[M::mem_process_seqs] Processed 675496 reads in 98.880 CPU sec, 3.098 real sec
sorting block 114, #reads = 1062658 mem = 536877218 peak = 1139998720
[M::process] read 675348 sequences (100000229 bp) in 2.476 CPU sec, 2.511 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281248, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 387, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068)
[M::mem_pestat] mean and std.dev: (396.27, 180.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1340)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 115, #reads = 1063363 mem = 536879684 peak = 1139998720
[M::process] read 674360 sequences (100000216 bp) in 2.802 CPU sec, 2.870 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280894, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 388, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1058)
[M::mem_pestat] mean and std.dev: (396.88, 178.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1326)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675620 reads in 159.136 CPU sec, 5.037 real sec
[M::mem_process_seqs] Processed 675348 reads in 102.784 CPU sec, 3.229 real sec
[M::process] read 674416 sequences (100000036 bp) in 2.516 CPU sec, 2.550 real sec...
sorting block 116, #reads = 1062923 mem = 536878832 peak = 1139998720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280753, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 394, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052)
[M::mem_pestat] mean and std.dev: (401.94, 178.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1315)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674608 sequences (100000263 bp) in 2.644 CPU sec, 2.718 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280350, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 396, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1057)
[M::mem_pestat] mean and std.dev: (402.90, 178.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1322)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674360 reads in 155.026 CPU sec, 4.916 real sec
[M::mem_process_seqs] Processed 674416 reads in 100.650 CPU sec, 3.160 real sec
[M::process] read 674122 sequences (100000231 bp) in 2.580 CPU sec, 2.633 real sec...
sorting block 117, #reads = 1064393 mem = 536879381 peak = 1139998720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281306, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 400, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068)
[M::mem_pestat] mean and std.dev: (405.80, 180.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1336)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675102 sequences (100000098 bp) in 2.741 CPU sec, 2.808 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280546, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 402, 535)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075)
[M::mem_pestat] mean and std.dev: (408.56, 181.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1345)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674608 reads in 159.520 CPU sec, 5.050 real sec
[M::mem_process_seqs] Processed 674122 reads in 103.522 CPU sec, 3.252 real sec
sorting block 118, #reads = 1062583 mem = 536877304 peak = 1139998720
[M::process] read 676486 sequences (100000024 bp) in 2.543 CPU sec, 2.576 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281367, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 393, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062)
[M::mem_pestat] mean and std.dev: (400.11, 179.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1330)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675898 sequences (100000123 bp) in 2.640 CPU sec, 2.706 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281455, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 374, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047)
[M::mem_pestat] mean and std.dev: (385.76, 177.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1315)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675102 reads in 157.517 CPU sec, 4.991 real sec
sorting block 119, #reads = 1064638 mem = 536877205 peak = 1139998720
[M::mem_process_seqs] Processed 676486 reads in 101.630 CPU sec, 3.192 real sec
[M::process] read 675530 sequences (100000041 bp) in 2.611 CPU sec, 2.649 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 281170, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 383, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1057)
[M::mem_pestat] mean and std.dev: (392.52, 178.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1326)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 120, #reads = 1064831 mem = 536882547 peak = 1139998720
[M::process] read 675440 sequences (100000245 bp) in 2.702 CPU sec, 2.767 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281580, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 388, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1061)
[M::mem_pestat] mean and std.dev: (396.77, 179.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1330)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675898 reads in 156.727 CPU sec, 4.963 real sec
[M::mem_process_seqs] Processed 675530 reads in 100.985 CPU sec, 3.173 real sec
[M::process] read 675442 sequences (100000269 bp) in 2.658 CPU sec, 2.705 real sec...
sorting block 121, #reads = 1064255 mem = 536882099 peak = 1139998720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281576, 0, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 387, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060)
[M::mem_pestat] mean and std.dev: (395.49, 178.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1329)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 415, 753)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1767)
[M::mem_pestat] mean and std.dev: (425.92, 249.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2274)
[M::mem_pestat] skip orientation RR
[M::process] read 674858 sequences (100000020 bp) in 2.521 CPU sec, 2.590 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282286, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 388, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1058)
[M::mem_pestat] mean and std.dev: (396.30, 178.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1326)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675440 reads in 157.843 CPU sec, 5.005 real sec
[M::mem_process_seqs] Processed 675442 reads in 103.511 CPU sec, 3.255 real sec
[M::process] read 674486 sequences (100000205 bp) in 2.082 CPU sec, 2.117 real sec...
sorting block 122, #reads = 1062757 mem = 536878196 peak = 1139998720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281248, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 392, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1071)
[M::mem_pestat] mean and std.dev: (401.41, 181.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1342)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674812 sequences (100000102 bp) in 2.638 CPU sec, 2.710 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280981, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 394, 531)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075)
[M::mem_pestat] mean and std.dev: (403.58, 182.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1347)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674858 reads in 159.349 CPU sec, 5.043 real sec
[M::mem_process_seqs] Processed 674486 reads in 104.232 CPU sec, 3.273 real sec
sorting block 123, #reads = 1062294 mem = 536879273 peak = 1139998720
[M::process] read 675202 sequences (100000090 bp) in 2.529 CPU sec, 2.549 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281189, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 393, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1072)
[M::mem_pestat] mean and std.dev: (403.33, 182.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1343)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675724 sequences (100000194 bp) in 2.680 CPU sec, 2.754 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281938, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 385, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1057)
[M::mem_pestat] mean and std.dev: (395.44, 179.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1325)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674812 reads in 157.494 CPU sec, 4.980 real sec
sorting block 124, #reads = 1063631 mem = 536879019 peak = 1139998720
[M::mem_process_seqs] Processed 675202 reads in 103.549 CPU sec, 3.248 real sec
[M::process] read 675300 sequences (100000005 bp) in 2.512 CPU sec, 2.545 real sec...
sorting block 125, #reads = 1064152 mem = 536880149 peak = 1139998720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281683, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 379, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053)
[M::mem_pestat] mean and std.dev: (391.31, 179.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1321)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674924 sequences (100000167 bp) in 2.818 CPU sec, 2.883 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 281829, 0, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (400, 408, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (384, 424)
[M::mem_pestat] mean and std.dev: (405.60, 3.67)
[M::mem_pestat] low and high boundaries for proper pairs: (376, 432)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 387, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075)
[M::mem_pestat] mean and std.dev: (398.10, 182.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1349)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675724 reads in 161.030 CPU sec, 5.095 real sec
[M::mem_process_seqs] Processed 675300 reads in 103.612 CPU sec, 3.249 real sec
[M::process] read 674944 sequences (100000163 bp) in 2.427 CPU sec, 2.443 real sec...
sorting block 126, #reads = 1059503 mem = 536878329 peak = 1139998720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281233, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 390, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1080)
[M::mem_pestat] mean and std.dev: (401.26, 183.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1355)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675362 sequences (100000121 bp) in 2.563 CPU sec, 2.623 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281983, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 390, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1080)
[M::mem_pestat] mean and std.dev: (400.77, 182.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1355)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674924 reads in 159.157 CPU sec, 5.028 real sec
[M::mem_process_seqs] Processed 674944 reads in 103.016 CPU sec, 3.222 real sec
[M::process] read 675080 sequences (100000269 bp) in 2.221 CPU sec, 2.231 real sec...
sorting block 127, #reads = 1062468 mem = 536878545 peak = 1139998720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281257, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 386, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1077)
[M::mem_pestat] mean and std.dev: (398.21, 183.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1352)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675060 sequences (100000159 bp) in 2.502 CPU sec, 2.565 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281369, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 390, 534)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1096)
[M::mem_pestat] mean and std.dev: (403.25, 187.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1377)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675718 sequences (100000075 bp) in 1.670 CPU sec, 1.667 real sec...
[M::mem_process_seqs] Processed 675362 reads in 163.551 CPU sec, 5.184 real sec
sorting block 128, #reads = 1062521 mem = 536878993 peak = 1139998720
[M::mem_process_seqs] Processed 675080 reads in 106.581 CPU sec, 3.367 real sec
sorting block 129, #reads = 1061395 mem = 536879105 peak = 1139998720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281836, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 389, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088)
[M::mem_pestat] mean and std.dev: (402.07, 185.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1366)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675494 sequences (100000166 bp) in 2.514 CPU sec, 2.581 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281951, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 379, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1073)
[M::mem_pestat] mean and std.dev: (394.71, 183.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1348)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675060 reads in 167.601 CPU sec, 5.287 real sec
[M::process] read 678588 sequences (100000280 bp) in 2.280 CPU sec, 2.284 real sec...
[M::mem_process_seqs] Processed 675718 reads in 111.204 CPU sec, 3.501 real sec
sorting block 130, #reads = 1061591 mem = 536878259 peak = 1139998720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281309, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 381, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1072)
[M::mem_pestat] mean and std.dev: (395.78, 182.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1346)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678220 sequences (100000046 bp) in 2.640 CPU sec, 2.686 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282669, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 358, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046)
[M::mem_pestat] mean and std.dev: (374.97, 179.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1318)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676946 sequences (100000060 bp) in 2.320 CPU sec, 2.322 real sec...
[M::mem_process_seqs] Processed 675494 reads in 174.542 CPU sec, 5.503 real sec
[M::mem_process_seqs] Processed 678588 reads in 114.006 CPU sec, 3.597 real sec
sorting block 131, #reads = 1056266 mem = 536878687 peak = 1139998720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282963, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 364, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (377.19, 177.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1313)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676420 sequences (100000245 bp) in 2.743 CPU sec, 2.800 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282421, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 376, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1059)
[M::mem_pestat] mean and std.dev: (387.16, 178.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1331)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675682 sequences (100000177 bp) in 2.170 CPU sec, 2.173 real sec...
[M::mem_process_seqs] Processed 678220 reads in 167.250 CPU sec, 5.269 real sec
sorting block 132, #reads = 1057656 mem = 536881708 peak = 1139998720
[M::mem_process_seqs] Processed 676946 reads in 107.008 CPU sec, 3.371 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282108, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 383, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1063)
[M::mem_pestat] mean and std.dev: (391.43, 178.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1335)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675770 sequences (100000021 bp) in 3.014 CPU sec, 3.080 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281586, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 387, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048)
[M::mem_pestat] mean and std.dev: (393.68, 176.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1313)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676420 reads in 164.312 CPU sec, 5.182 real sec
sorting block 133, #reads = 1061319 mem = 536878449 peak = 1139998720
[M::mem_process_seqs] Processed 675682 reads in 106.611 CPU sec, 3.344 real sec
[M::process] read 675106 sequences (100000062 bp) in 2.443 CPU sec, 2.444 real sec...
sorting block 134, #reads = 1060106 mem = 536878308 peak = 1139998720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281657, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 385, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052)
[M::mem_pestat] mean and std.dev: (392.46, 176.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1319)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674996 sequences (100000086 bp) in 2.582 CPU sec, 2.647 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282053, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 393, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045)
[M::mem_pestat] mean and std.dev: (397.01, 174.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1307)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675770 reads in 160.859 CPU sec, 5.091 real sec
[M::mem_process_seqs] Processed 675106 reads in 106.819 CPU sec, 3.344 real sec
[M::process] read 674600 sequences (100000056 bp) in 2.392 CPU sec, 2.392 real sec...
sorting block 135, #reads = 1060514 mem = 536884818 peak = 1139998720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281496, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 396, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1061)
[M::mem_pestat] mean and std.dev: (400.16, 176.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1328)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675730 sequences (100000161 bp) in 2.886 CPU sec, 2.950 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280572, 3, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 398, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060)
[M::mem_pestat] mean and std.dev: (402.41, 177.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1326)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (348, 348, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (348, 348)
[M::mem_pestat] mean and std.dev: (348.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (348, 348)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 674996 reads in 164.614 CPU sec, 5.202 real sec
[M::process] read 675494 sequences (100000273 bp) in 1.849 CPU sec, 1.856 real sec...
[M::mem_process_seqs] Processed 674600 reads in 108.120 CPU sec, 3.408 real sec
sorting block 136, #reads = 1059738 mem = 536879452 peak = 1139998720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281585, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 383, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (391.17, 175.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1307)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674872 sequences (100000038 bp) in 2.646 CPU sec, 2.704 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281421, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 381, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (389.92, 173.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1296)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675730 reads in 164.167 CPU sec, 5.185 real sec
[M::process] read 674980 sequences (100000177 bp) in 2.227 CPU sec, 2.231 real sec...
sorting block 137, #reads = 1060610 mem = 536880720 peak = 1139998720
[M::mem_process_seqs] Processed 675494 reads in 106.052 CPU sec, 3.332 real sec
sorting block 138, #reads = 1061774 mem = 536877779 peak = 1139998720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280860, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 387, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040)
[M::mem_pestat] mean and std.dev: (394.29, 174.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1301)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674942 sequences (100000157 bp) in 2.886 CPU sec, 2.935 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280919, 8, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 386, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (393.64, 173.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1300)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 722, 722)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1736)
[M::mem_pestat] mean and std.dev: (470.00, 271.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2243)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 674872 reads in 167.413 CPU sec, 5.284 real sec
[M::process] read 675178 sequences (100000133 bp) in 2.405 CPU sec, 2.411 real sec...
[M::mem_process_seqs] Processed 674980 reads in 108.384 CPU sec, 3.398 real sec
sorting block 139, #reads = 1061667 mem = 536878517 peak = 1139998720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281282, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 385, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (392.13, 172.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674852 sequences (100000257 bp) in 2.684 CPU sec, 2.742 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281464, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 376, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (384.25, 167.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674942 reads in 168.983 CPU sec, 5.340 real sec
[M::process] read 674630 sequences (100000051 bp) in 2.316 CPU sec, 2.318 real sec...
[M::mem_process_seqs] Processed 675178 reads in 112.075 CPU sec, 3.517 real sec
sorting block 140, #reads = 1062584 mem = 536885949 peak = 1139998720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280894, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 379, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (387.38, 169.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674518 sequences (100000070 bp) in 2.761 CPU sec, 2.825 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280946, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 378, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (388.06, 170.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674852 reads in 168.027 CPU sec, 5.300 real sec
[M::process] read 674502 sequences (100000097 bp) in 2.270 CPU sec, 2.264 real sec...
sorting block 141, #reads = 1059500 mem = 536879319 peak = 1139998720
[M::mem_process_seqs] Processed 674630 reads in 109.342 CPU sec, 3.438 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280484, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 378, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (388.60, 171.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674384 sequences (100000019 bp) in 2.093 CPU sec, 2.172 real sec...
[M::process] read 674660 sequences (100000160 bp) in 1.720 CPU sec, 1.720 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280308, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 379, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (389.18, 171.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674518 reads in 166.148 CPU sec, 5.273 real sec
sorting block 142, #reads = 1061448 mem = 536880654 peak = 1139998720
[M::process] read 674776 sequences (100000086 bp) in 2.023 CPU sec, 2.027 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280685, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 376, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (386.50, 169.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674384 reads in 93.220 CPU sec, 2.950 real sec
sorting block 143, #reads = 1062042 mem = 536880089 peak = 1139998720
[M::mem_process_seqs] Processed 674502 reads in 229.229 CPU sec, 7.278 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280867, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 377, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (388.65, 172.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674396 sequences (100000107 bp) in 2.230 CPU sec, 2.336 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280568, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 374, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (386.58, 172.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674490 sequences (100000207 bp) in 1.750 CPU sec, 1.754 real sec...
[M::mem_process_seqs] Processed 674660 reads in 182.001 CPU sec, 5.773 real sec
sorting block 144, #reads = 1062137 mem = 536880092 peak = 1139998720
[M::mem_process_seqs] Processed 674776 reads in 107.904 CPU sec, 3.410 real sec
sorting block 145, #reads = 1062840 mem = 536880680 peak = 1139998720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 280600, 2, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 288, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534)
[M::mem_pestat] mean and std.dev: (236.78, 65.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 654)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 378, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (390.14, 173.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674436 sequences (100000076 bp) in 2.326 CPU sec, 2.425 real sec...
[M::process] read 674468 sequences (100000146 bp) in 1.650 CPU sec, 1.649 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280155, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 379, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (390.49, 172.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674396 reads in 166.416 CPU sec, 5.282 real sec
[M::mem_process_seqs] Processed 674490 reads in 108.280 CPU sec, 3.438 real sec
sorting block 146, #reads = 1063587 mem = 536881523 peak = 1139998720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280665, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 380, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (391.07, 173.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674408 sequences (100000277 bp) in 2.566 CPU sec, 2.634 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280362, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 380, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (392.22, 174.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 250, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479)
[M::mem_pestat] mean and std.dev: (223.55, 85.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 590)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 674436 reads in 159.868 CPU sec, 5.042 real sec
[M::process] read 674150 sequences (100000125 bp) in 2.267 CPU sec, 2.266 real sec...
[M::mem_process_seqs] Processed 674468 reads in 101.717 CPU sec, 3.193 real sec
sorting block 147, #reads = 1063447 mem = 536878431 peak = 1139998720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280768, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 383, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037)
[M::mem_pestat] mean and std.dev: (395.19, 177.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1297)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 486286 sequences (72051389 bp) in 2.007 CPU sec, 2.093 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 280919, 2, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 268, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (277.00, 140.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 385, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (397.33, 177.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 674408 reads in 154.655 CPU sec, 4.965 real sec
[M::mem_process_seqs] Processed 674150 reads in 98.842 CPU sec, 3.189 real sec
sorting block 148, #reads = 1064023 mem = 536880102 peak = 1139998720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 202870, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 380, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (391.82, 176.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1297)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 486286 reads in 64.325 CPU sec, 2.129 real sec
sorting block 149, #reads = 1063292 mem = 536879341 peak = 1173663744
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: 1f8bfbff fffab20b
  extended: f1bf07ab 1a407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 159121836
overall: 69681143808 mem 23176.354 user 536.738 sys 828.114 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1.L001\tPU:L001\tSM:FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1\tLB:FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1_R1.fastq.gz FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 828.141 sec; CPU: 23713.095 sec
sorting block 150, #reads = 393273 mem = 199080365 peak = 1173663744
uniq header count 1
merging 151 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: 1f8bfbff fffab20b
  extended: f1bf07ab 1a407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8559C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 8901386288
output reads: 159841354
bam_mem_sort: 151 calls 87.513 user 1.640 sys 89.938 real
bam_write: 151 calls 48.398 user 2.770 sys 68.639 real
execute: 1 calls 45.754 user 1.298 sys 900.279 real
merge_files: 1 calls 45.713 user 1.277 sys 71.892 real
parse_chunk: 2406 calls 135.186 user 30.569 sys 174.899 real
read_chunk: 6491 calls 72.451 user 43.435 sys 760.109 real
sort_block: 1 calls 48.245 user 42.661 sys 727.661 real
write_chunk: 4083 calls 45.313 user 0.979 sys 70.517 real
overall: 3169845248 mem 2779.601 user 96.757 sys 900.295 real
ls: cannot access '*.cram': No such file or directory