.command.log - Workdir - DAQ:SENTIEON_BWAMEM (1047_JG-T1-TDNA-1_A23MHWWLT4_1;L001;0001)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/54/0eec3dbed456e6891d8c3531221ede/.command.log
Size: 139.7 KB
Task: DAQ:SENTIEON_BWAMEM (1047_JG-T1-TDNA-1_A23MHWWLT4_1;L001;0001)
Attempt
Content

  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/54/0eec3dbed456e6891d8c3531221ede/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/88/93ad25caa6d19e5b4671f89b9976e6/1047_JG-T1-TDNA-1_A23MHWWLT4_1/0001.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/54/0eec3dbed456e6891d8c3531221ede/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/88/93ad25caa6d19e5b4671f89b9976e6/1047_JG-T1-TDNA-1_A23MHWWLT4_1/0001.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
==> STAGING COMPLETE (7 inputs)

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1047_JG-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1762.867 CPU sec, 123.168 real sec
[M::process] read 700480 sequences (100000197 bp) in 1.030 CPU sec, 1.034 real sec...
[M::process] read 699356 sequences (100000258 bp) in 1.370 CPU sec, 1.392 real sec...
[M::process] read 698572 sequences (100000301 bp) in 1.361 CPU sec, 1.375 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291761, 8, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (247.16, 119.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 237, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (260.73, 147.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RR
[M::process] read 698172 sequences (100000252 bp) in 1.230 CPU sec, 1.247 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290268, 2, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 210, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (33, 358)
[M::mem_pestat] mean and std.dev: (172.50, 51.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 423)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 229, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (253.48, 122.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291275, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (251.59, 121.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700480 reads in 232.857 CPU sec, 7.502 real sec
[M::mem_process_seqs] Processed 698572 reads in 146.969 CPU sec, 4.838 real sec
[M::mem_process_seqs] Processed 699356 reads in 160.782 CPU sec, 5.335 real sec
sorting block 0, #reads = 1094291 mem = 536880942 peak = 586133504
[M::process] read 697292 sequences (100000132 bp) in 1.955 CPU sec, 2.150 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291326, 1, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 257, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1247)
[M::mem_pestat] mean and std.dev: (228.20, 129.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1611)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (254.84, 123.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697412 sequences (100000068 bp) in 1.461 CPU sec, 1.495 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290504, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 232, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (257.03, 124.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 864)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698172 reads in 132.207 CPU sec, 4.235 real sec
sorting block 1, #reads = 1094015 mem = 536878216 peak = 801120256
[M::mem_process_seqs] Processed 697292 reads in 82.875 CPU sec, 2.618 real sec
[M::process] read 698146 sequences (100000181 bp) in 1.425 CPU sec, 1.472 real sec...
sorting block 2, #reads = 1094210 mem = 536880046 peak = 879779840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290918, 11, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 367, 658)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1612)
[M::mem_pestat] mean and std.dev: (443.60, 272.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2089)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 233, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (258.45, 125.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 876)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (869, 869, 2060)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4442)
[M::mem_pestat] mean and std.dev: (1248.18, 773.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5633)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::process] read 697988 sequences (100000254 bp) in 1.935 CPU sec, 2.064 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291047, 11, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 586, 1140)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3016)
[M::mem_pestat] mean and std.dev: (521.44, 352.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3954)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684)
[M::mem_pestat] mean and std.dev: (255.46, 124.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (376, 485, 715)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1393)
[M::mem_pestat] mean and std.dev: (515.18, 245.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1732)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::process] read 697578 sequences (100000239 bp) in 1.300 CPU sec, 1.307 real sec...
[M::mem_process_seqs] Processed 697412 reads in 131.087 CPU sec, 4.180 real sec
[M::mem_process_seqs] Processed 698146 reads in 82.034 CPU sec, 2.611 real sec
sorting block 3, #reads = 1093637 mem = 536877585 peak = 879779840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290933, 4, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (192.00, 70.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (254.69, 123.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697352 sequences (100000070 bp) in 2.047 CPU sec, 2.173 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290794, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (255.78, 123.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 856)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696812 sequences (100000058 bp) in 1.387 CPU sec, 1.391 real sec...
[M::mem_process_seqs] Processed 697988 reads in 131.410 CPU sec, 4.188 real sec
[M::mem_process_seqs] Processed 697578 reads in 81.921 CPU sec, 2.600 real sec
sorting block 4, #reads = 1094373 mem = 536878417 peak = 879779840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290676, 6, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (255.17, 122.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 852)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 232, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560)
[M::mem_pestat] mean and std.dev: (212.15, 73.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 695)
[M::mem_pestat] skip orientation RR
[M::process] read 697290 sequences (100000097 bp) in 2.144 CPU sec, 2.270 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 290660, 3, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 264, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (308.83, 141.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1302)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (254.97, 121.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697260 sequences (100000266 bp) in 1.438 CPU sec, 1.433 real sec...
[M::mem_process_seqs] Processed 697352 reads in 131.735 CPU sec, 4.196 real sec
sorting block 5, #reads = 1093775 mem = 536876422 peak = 879779840
[M::mem_process_seqs] Processed 696812 reads in 82.136 CPU sec, 2.601 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290949, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.29, 119.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 6, #reads = 1093225 mem = 536882567 peak = 879779840
[M::process] read 696526 sequences (100000282 bp) in 2.367 CPU sec, 2.500 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290429, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.32, 118.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696114 sequences (100000106 bp) in 1.529 CPU sec, 1.523 real sec...
[M::mem_process_seqs] Processed 697290 reads in 137.169 CPU sec, 4.362 real sec
[M::mem_process_seqs] Processed 697260 reads in 87.208 CPU sec, 2.759 real sec
sorting block 7, #reads = 1091285 mem = 536877095 peak = 879779840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290494, 10, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (252.56, 118.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (679, 689, 1560)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3322)
[M::mem_pestat] mean and std.dev: (742.67, 519.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4203)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 695608 sequences (100000041 bp) in 2.694 CPU sec, 2.839 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290247, 7, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (253.27, 118.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696526 reads in 134.526 CPU sec, 4.291 real sec
[M::mem_process_seqs] Processed 696114 reads in 84.490 CPU sec, 2.670 real sec
[M::process] read 695430 sequences (100000170 bp) in 2.018 CPU sec, 2.101 real sec...
sorting block 8, #reads = 1093114 mem = 536878732 peak = 879779840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 289551, 8, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (254.70, 119.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 176, 265)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501)
[M::mem_pestat] mean and std.dev: (186.36, 44.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 619)
[M::mem_pestat] skip orientation RR
[M::process] read 695472 sequences (100000129 bp) in 2.455 CPU sec, 2.607 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 289626, 11, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (254.81, 119.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (49, 169, 671)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1915)
[M::mem_pestat] mean and std.dev: (269.90, 310.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2537)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 695608 reads in 138.079 CPU sec, 4.418 real sec
sorting block 9, #reads = 1091650 mem = 536877593 peak = 879779840
[M::process] read 698718 sequences (100000049 bp) in 2.247 CPU sec, 2.369 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 289969, 3, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 233, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (255.48, 119.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 185, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (257.00, 150.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695472 reads in 77.676 CPU sec, 2.459 real sec
[M::process] read 699100 sequences (100000264 bp) in 1.995 CPU sec, 2.063 real sec...
[M::mem_process_seqs] Processed 695430 reads in 199.202 CPU sec, 6.318 real sec
sorting block 10, #reads = 1090988 mem = 536880916 peak = 879779840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291030, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.28, 119.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698696 sequences (100000174 bp) in 2.528 CPU sec, 2.704 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291490, 2, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (250.93, 120.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (306, 382, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (312.45, 144.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698718 reads in 153.150 CPU sec, 4.877 real sec
[M::mem_process_seqs] Processed 699100 reads in 88.274 CPU sec, 2.807 real sec
sorting block 11, #reads = 1092241 mem = 536878332 peak = 879779840
[M::process] read 698432 sequences (100000108 bp) in 2.290 CPU sec, 2.453 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291461, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (253.48, 122.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290703, 10, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 697308 sequences (100000024 bp) in 2.473 CPU sec, 2.676 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (158, 229, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (255.39, 124.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (959, 4398, 4398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11276)
[M::mem_pestat] mean and std.dev: (2588.20, 1841.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14715)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 698696 reads in 140.206 CPU sec, 4.520 real sec
[M::mem_process_seqs] Processed 698432 reads in 88.243 CPU sec, 2.812 real sec
sorting block 12, #reads = 1095468 mem = 536878957 peak = 879779840
[M::process] read 697044 sequences (100000115 bp) in 2.400 CPU sec, 2.595 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290846, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 232, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (257.61, 124.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 868)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 468, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (439.20, 303.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1655)
[M::mem_pestat] skip orientation RR
sorting block 13, #reads = 1095024 mem = 536879249 peak = 879779840
[M::process] read 697154 sequences (100000088 bp) in 2.181 CPU sec, 2.326 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291039, 8, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 234, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (259.38, 126.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 877)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 222, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690)
[M::mem_pestat] mean and std.dev: (249.33, 67.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 852)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697308 reads in 140.916 CPU sec, 4.542 real sec
[M::mem_process_seqs] Processed 697044 reads in 88.389 CPU sec, 2.815 real sec
[M::process] read 697674 sequences (100000007 bp) in 2.284 CPU sec, 2.462 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 290741, 7, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (273, 347, 556)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1122)
[M::mem_pestat] mean and std.dev: (357.06, 137.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1405)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 232, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (258.21, 125.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 872)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 14, #reads = 1095092 mem = 536877674 peak = 879779840
[M::process] read 697854 sequences (100000025 bp) in 2.146 CPU sec, 2.273 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291375, 6, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (232, 256, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (259.20, 84.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 780)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (255.27, 123.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 406, 667)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1461)
[M::mem_pestat] mean and std.dev: (417.90, 229.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1858)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697154 reads in 139.921 CPU sec, 4.494 real sec
[M::mem_process_seqs] Processed 697674 reads in 87.402 CPU sec, 2.780 real sec
sorting block 15, #reads = 1094696 mem = 536880144 peak = 879779840
[M::process] read 697246 sequences (100000208 bp) in 2.188 CPU sec, 2.332 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291018, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (254.81, 123.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696970 sequences (100000157 bp) in 2.439 CPU sec, 2.632 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 290436, 1, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.18, 121.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 310, 817)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2027)
[M::mem_pestat] mean and std.dev: (483.58, 347.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2632)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697854 reads in 143.668 CPU sec, 4.623 real sec
[M::mem_process_seqs] Processed 697246 reads in 89.917 CPU sec, 2.869 real sec
sorting block 16, #reads = 1093654 mem = 536876733 peak = 879779840
[M::process] read 697544 sequences (100000291 bp) in 2.411 CPU sec, 2.575 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290678, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (253.40, 120.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698436 sequences (100000282 bp) in 2.319 CPU sec, 2.460 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290476, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (251.92, 120.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696970 reads in 142.063 CPU sec, 4.556 real sec
sorting block 17, #reads = 1094052 mem = 536878412 peak = 879779840
[M::mem_process_seqs] Processed 697544 reads in 88.722 CPU sec, 2.813 real sec
[M::process] read 697136 sequences (100000129 bp) in 2.406 CPU sec, 2.563 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 290757, 2, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (211, 264, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553)
[M::mem_pestat] mean and std.dev: (236.85, 74.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 667)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (248.95, 117.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 18, #reads = 1093470 mem = 536878868 peak = 879779840
[M::process] read 696686 sequences (100000023 bp) in 2.364 CPU sec, 2.540 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290692, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (252.50, 120.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698436 reads in 145.116 CPU sec, 4.653 real sec
[M::mem_process_seqs] Processed 697136 reads in 89.410 CPU sec, 2.837 real sec
[M::process] read 695886 sequences (100000134 bp) in 2.473 CPU sec, 2.632 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290242, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (253.27, 119.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 19, #reads = 1091146 mem = 536882677 peak = 879779840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290045, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (253.89, 119.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 695834 sequences (100000023 bp) in 2.408 CPU sec, 2.579 real sec...
[M::mem_process_seqs] Processed 696686 reads in 138.438 CPU sec, 4.443 real sec
[M::mem_process_seqs] Processed 695886 reads in 87.286 CPU sec, 2.779 real sec
sorting block 20, #reads = 1092698 mem = 536882205 peak = 879779840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 290080, 6, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (221, 286, 539)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1175)
[M::mem_pestat] mean and std.dev: (280.55, 106.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1493)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (255.08, 120.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 695634 sequences (100000131 bp) in 2.452 CPU sec, 2.600 real sec...
[M::mem_process_seqs] Processed 695834 reads in 75.131 CPU sec, 2.486 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290114, 13, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (343, 655, 2157)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5785)
[M::mem_pestat] mean and std.dev: (1069.58, 870.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7599)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 234, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (256.31, 121.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (75, 181, 555)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1515)
[M::mem_pestat] mean and std.dev: (287.15, 281.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1995)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 286, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 525)
[M::mem_pestat] mean and std.dev: (262.83, 68.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 623)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 698868 sequences (100000130 bp) in 2.422 CPU sec, 2.587 real sec...
[M::mem_process_seqs] Processed 695634 reads in 75.273 CPU sec, 2.497 real sec
sorting block 21, #reads = 1092226 mem = 536880263 peak = 879779840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291335, 6, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 186, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515)
[M::mem_pestat] mean and std.dev: (193.10, 59.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 629)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.20, 120.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 209, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575)
[M::mem_pestat] mean and std.dev: (235.45, 121.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 721)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 699396 sequences (100000198 bp) in 2.453 CPU sec, 2.621 real sec...
[M::mem_process_seqs] Processed 698868 reads in 76.826 CPU sec, 2.535 real sec
sorting block 22, #reads = 1091707 mem = 536879938 peak = 879779840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291275, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (250.80, 121.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699396 reads in 76.591 CPU sec, 2.526 real sec
[M::process] read 698168 sequences (100000035 bp) in 2.479 CPU sec, 2.658 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291027, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (254.12, 122.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 168, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (202.10, 57.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 704)
[M::mem_pestat] skip orientation RR
sorting block 23, #reads = 1093388 mem = 536879534 peak = 879779840
[M::mem_process_seqs] Processed 698168 reads in 75.746 CPU sec, 2.501 real sec
[M::process] read 697666 sequences (100000177 bp) in 2.482 CPU sec, 2.651 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290800, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 231, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687)
[M::mem_pestat] mean and std.dev: (255.97, 123.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697666 reads in 76.007 CPU sec, 2.488 real sec
[M::process] read 696780 sequences (100000190 bp) in 2.373 CPU sec, 2.566 real sec...
sorting block 24, #reads = 1094596 mem = 536877991 peak = 879779840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 290354, 1, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (115, 190, 537)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1381)
[M::mem_pestat] mean and std.dev: (278.46, 248.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1803)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 233, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (258.94, 125.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 873)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696780 reads in 75.898 CPU sec, 2.473 real sec
[M::process] read 696656 sequences (100000225 bp) in 2.503 CPU sec, 2.730 real sec...
sorting block 25, #reads = 1093905 mem = 536883344 peak = 879779840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290937, 7, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 235, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (260.44, 126.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 881)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 237, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (251.10, 79.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696656 reads in 72.980 CPU sec, 2.385 real sec
[M::process] read 696720 sequences (100000207 bp) in 2.592 CPU sec, 2.776 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290374, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 234, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (259.57, 126.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 877)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697920 sequences (100000131 bp) in 2.241 CPU sec, 2.394 real sec...
[M::mem_process_seqs] Processed 696720 reads in 74.193 CPU sec, 2.398 real sec
sorting block 26, #reads = 1093363 mem = 536879395 peak = 879779840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290978, 13, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (253.60, 122.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (78, 115, 1254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3606)
[M::mem_pestat] mean and std.dev: (397.82, 422.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4782)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 697208 sequences (100000036 bp) in 2.125 CPU sec, 2.257 real sec...
[M::mem_process_seqs] Processed 697920 reads in 74.498 CPU sec, 2.412 real sec
sorting block 27, #reads = 1093159 mem = 536878668 peak = 879779840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290479, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (255.49, 123.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 856)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696886 sequences (100000146 bp) in 2.098 CPU sec, 2.231 real sec...
[M::mem_process_seqs] Processed 697208 reads in 74.238 CPU sec, 2.395 real sec
[M::process] read 697210 sequences (100000149 bp) in 2.111 CPU sec, 2.243 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290553, 5, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 424, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (326.22, 140.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1098)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (254.83, 122.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (317, 366, 1569)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4073)
[M::mem_pestat] mean and std.dev: (728.31, 900.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5325)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 697296 sequences (100000123 bp) in 1.348 CPU sec, 1.343 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290393, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (252.74, 120.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696886 reads in 134.233 CPU sec, 4.222 real sec
[M::mem_process_seqs] Processed 697210 reads in 83.407 CPU sec, 2.619 real sec
sorting block 28, #reads = 1093774 mem = 536877711 peak = 879779840
[M::process] read 697634 sequences (100000211 bp) in 1.799 CPU sec, 1.864 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290114, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.30, 120.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 29, #reads = 1093166 mem = 536876815 peak = 970989568
[M::process] read 697070 sequences (100000211 bp) in 2.588 CPU sec, 2.751 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290612, 4, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 243, 1407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3879)
[M::mem_pestat] mean and std.dev: (512.20, 510.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5115)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (250.88, 119.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697296 reads in 136.302 CPU sec, 4.349 real sec
[M::mem_process_seqs] Processed 697634 reads in 86.912 CPU sec, 2.747 real sec
[M::process] read 696486 sequences (100000214 bp) in 2.060 CPU sec, 2.183 real sec...
sorting block 30, #reads = 1092820 mem = 536880593 peak = 970989568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290390, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (252.72, 120.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696196 sequences (100000287 bp) in 2.068 CPU sec, 2.178 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290238, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (253.62, 120.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 724)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1852)
[M::mem_pestat] mean and std.dev: (405.10, 360.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2416)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697070 reads in 136.017 CPU sec, 4.322 real sec
[M::mem_process_seqs] Processed 696486 reads in 85.132 CPU sec, 2.683 real sec
[M::process] read 696082 sequences (100000231 bp) in 1.652 CPU sec, 1.688 real sec...
sorting block 31, #reads = 1091335 mem = 536884778 peak = 970989568
[M::process] read 695822 sequences (100000029 bp) in 2.613 CPU sec, 2.768 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290360, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.99, 121.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 197, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (304.50, 256.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1332)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696082 reads in 74.047 CPU sec, 2.336 real sec
sorting block 32, #reads = 1092037 mem = 536878249 peak = 970989568
[M::process] read 697244 sequences (100000003 bp) in 2.054 CPU sec, 2.162 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290130, 6, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (751, 1084, 4639)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12415)
[M::mem_pestat] mean and std.dev: (2386.30, 2698.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16303)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (254.20, 120.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 437, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076)
[M::mem_pestat] mean and std.dev: (356.78, 123.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1359)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290090, 8, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (113, 239, 265)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569)
[M::mem_pestat] mean and std.dev: (210.56, 110.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 721)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (255.35, 121.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696196 reads in 209.281 CPU sec, 6.653 real sec
[M::mem_process_seqs] Processed 695822 reads in 98.163 CPU sec, 3.108 real sec
[M::process] read 698910 sequences (100000139 bp) in 2.048 CPU sec, 2.147 real sec...
sorting block 33, #reads = 1091926 mem = 536877708 peak = 970989568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290559, 4, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (253.25, 120.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 472, 653)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1475)
[M::mem_pestat] mean and std.dev: (472.38, 317.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1886)
[M::mem_pestat] skip orientation RR
[M::process] read 698380 sequences (100000275 bp) in 2.406 CPU sec, 2.584 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 290620, 3, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 182, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (209.20, 142.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (250.74, 120.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 193, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (240.89, 123.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697244 reads in 136.465 CPU sec, 4.352 real sec
[M::mem_process_seqs] Processed 698910 reads in 85.101 CPU sec, 2.692 real sec
sorting block 34, #reads = 1090677 mem = 536878468 peak = 970989568
[M::process] read 697316 sequences (100000210 bp) in 2.248 CPU sec, 2.376 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291233, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (254.18, 123.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696786 sequences (100000143 bp) in 2.376 CPU sec, 2.506 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290869, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (256.80, 124.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 864)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698380 reads in 134.241 CPU sec, 4.293 real sec
[M::mem_process_seqs] Processed 697316 reads in 84.285 CPU sec, 2.673 real sec
sorting block 35, #reads = 1094711 mem = 536884398 peak = 970989568
[M::process] read 695954 sequences (100000281 bp) in 2.495 CPU sec, 2.632 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290517, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (257.31, 124.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 865)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 36, #reads = 1094256 mem = 536878919 peak = 970989568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290369, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 236, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699)
[M::mem_pestat] mean and std.dev: (260.58, 126.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 695992 sequences (100000124 bp) in 2.375 CPU sec, 2.519 real sec...
[M::mem_process_seqs] Processed 696786 reads in 134.867 CPU sec, 4.327 real sec
[M::mem_process_seqs] Processed 695954 reads in 84.906 CPU sec, 2.702 real sec
[M::process] read 697730 sequences (100000215 bp) in 2.399 CPU sec, 2.544 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 289969, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 236, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705)
[M::mem_pestat] mean and std.dev: (261.48, 127.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 37, #reads = 1093903 mem = 536880272 peak = 970989568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290703, 10, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (254.12, 123.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (43, 233, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1258)
[M::mem_pestat] mean and std.dev: (398.60, 452.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2210)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 695992 reads in 137.176 CPU sec, 4.407 real sec
[M::process] read 696594 sequences (100000290 bp) in 2.275 CPU sec, 2.385 real sec...
[M::mem_process_seqs] Processed 697730 reads in 86.314 CPU sec, 2.752 real sec
sorting block 38, #reads = 1093309 mem = 536880239 peak = 970989568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290699, 6, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 180, 1084)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2932)
[M::mem_pestat] mean and std.dev: (441.83, 495.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3856)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (256.54, 124.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 853)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697168 sequences (100000056 bp) in 2.619 CPU sec, 2.768 real sec...
[M::mem_process_seqs] Processed 696594 reads in 75.782 CPU sec, 2.506 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290766, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (253.92, 122.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697168 reads in 75.738 CPU sec, 2.511 real sec
[M::process] read 697064 sequences (100000132 bp) in 2.596 CPU sec, 2.757 real sec...
sorting block 39, #reads = 1093988 mem = 536885396 peak = 970989568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 289963, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (252.53, 120.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697064 reads in 78.715 CPU sec, 2.612 real sec
[M::process] read 696872 sequences (100000112 bp) in 2.875 CPU sec, 3.069 real sec...
sorting block 40, #reads = 1094318 mem = 536878096 peak = 970989568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 289968, 4, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (253.39, 120.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 285, 575)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1207)
[M::mem_pestat] mean and std.dev: (426.54, 303.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1642)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696872 reads in 85.561 CPU sec, 2.815 real sec
[M::process] read 696764 sequences (100000135 bp) in 2.706 CPU sec, 2.907 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290427, 9, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (253.17, 120.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696578 sequences (100000193 bp) in 2.575 CPU sec, 2.743 real sec...
[M::mem_process_seqs] Processed 696764 reads in 83.414 CPU sec, 2.744 real sec
sorting block 41, #reads = 1092108 mem = 536881867 peak = 970989568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290398, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.69, 121.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696578 reads in 84.660 CPU sec, 2.780 real sec
[M::process] read 696968 sequences (100000289 bp) in 2.725 CPU sec, 2.904 real sec...
sorting block 42, #reads = 1093447 mem = 536884729 peak = 970989568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290326, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (252.72, 120.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 389, 3148)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9142)
[M::mem_pestat] mean and std.dev: (1394.40, 1742.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12139)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696968 reads in 86.518 CPU sec, 2.827 real sec
[M::process] read 696246 sequences (100000183 bp) in 2.712 CPU sec, 2.909 real sec...
sorting block 43, #reads = 1093225 mem = 536877454 peak = 970989568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290182, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.74, 121.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 695640 sequences (100000172 bp) in 2.600 CPU sec, 2.794 real sec...
[M::mem_process_seqs] Processed 696246 reads in 87.035 CPU sec, 2.846 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290304, 4, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 195, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (200.40, 83.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 699)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 234, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (256.84, 122.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 695640 reads in 85.644 CPU sec, 2.780 real sec
[M::process] read 695362 sequences (100000194 bp) in 2.893 CPU sec, 3.088 real sec...
sorting block 44, #reads = 1092203 mem = 536879476 peak = 970989568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290033, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 234, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (256.98, 122.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 695362 reads in 85.716 CPU sec, 2.761 real sec
[M::process] read 697414 sequences (100000198 bp) in 2.723 CPU sec, 2.884 real sec...
sorting block 45, #reads = 1092423 mem = 536878929 peak = 970989568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290314, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (252.73, 121.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704822 sequences (100000298 bp) in 2.408 CPU sec, 2.742 real sec...
[M::mem_process_seqs] Processed 697414 reads in 87.258 CPU sec, 2.810 real sec
[M::process] read 703786 sequences (100000058 bp) in 2.349 CPU sec, 2.489 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292810, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 209, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611)
[M::mem_pestat] mean and std.dev: (234.57, 113.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 765)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703352 sequences (100000219 bp) in 1.750 CPU sec, 1.751 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292267, 9, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (237.83, 115.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 782)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 704822 reads in 162.590 CPU sec, 5.132 real sec
[M::mem_process_seqs] Processed 703786 reads in 101.178 CPU sec, 3.179 real sec
sorting block 46, #reads = 1092835 mem = 536881170 peak = 970989568
[M::process] read 701730 sequences (100000254 bp) in 1.847 CPU sec, 1.910 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292623, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (240.80, 117.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 47, #reads = 1095064 mem = 536879390 peak = 1007878144
[M::process] read 701280 sequences (100000213 bp) in 2.855 CPU sec, 3.043 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291987, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (245.58, 120.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703352 reads in 160.344 CPU sec, 5.114 real sec
[M::mem_process_seqs] Processed 701730 reads in 99.870 CPU sec, 3.156 real sec
[M::process] read 702006 sequences (100000097 bp) in 1.955 CPU sec, 2.037 real sec...
sorting block 48, #reads = 1093843 mem = 536876822 peak = 1007878144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292007, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (247.94, 121.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703520 sequences (100000091 bp) in 2.533 CPU sec, 2.655 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292564, 8, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (244.62, 119.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703200 sequences (100000213 bp) in 1.478 CPU sec, 1.482 real sec...
[M::mem_process_seqs] Processed 701280 reads in 155.278 CPU sec, 4.934 real sec
[M::mem_process_seqs] Processed 702006 reads in 98.932 CPU sec, 3.131 real sec
sorting block 49, #reads = 1094514 mem = 536879027 peak = 1007878144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292735, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (239.50, 116.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702204 sequences (100000134 bp) in 3.003 CPU sec, 3.189 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292859, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (240.32, 116.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700298 sequences (100000178 bp) in 1.520 CPU sec, 1.519 real sec...
[M::mem_process_seqs] Processed 703520 reads in 167.684 CPU sec, 5.328 real sec
sorting block 50, #reads = 1093925 mem = 536878530 peak = 1007878144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292632, 4, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 156, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (20, 360)
[M::mem_pestat] mean and std.dev: (169.00, 35.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 428)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (243.34, 118.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 702204 reads in 92.902 CPU sec, 2.929 real sec
[M::process] read 702422 sequences (100000157 bp) in 2.878 CPU sec, 2.899 real sec...
[M::mem_process_seqs] Processed 703200 reads in 235.393 CPU sec, 7.453 real sec
sorting block 51, #reads = 1093064 mem = 536879120 peak = 1007878144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291925, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (247.41, 120.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700382 sequences (100000052 bp) in 2.993 CPU sec, 3.151 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291981, 10, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.49, 117.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (88, 194, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (153.62, 95.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1088)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 700298 reads in 187.796 CPU sec, 5.945 real sec
[M::mem_process_seqs] Processed 702422 reads in 118.348 CPU sec, 3.730 real sec
[M::process] read 699342 sequences (100000029 bp) in 2.024 CPU sec, 2.103 real sec...
sorting block 52, #reads = 1091878 mem = 536878211 peak = 1007878144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 291425, 4, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 164, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507)
[M::mem_pestat] mean and std.dev: (172.43, 62.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 627)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (245.38, 118.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 339, 1152)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2958)
[M::mem_pestat] mean and std.dev: (463.45, 369.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3861)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 699214 sequences (100000058 bp) in 3.048 CPU sec, 3.228 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291582, 13, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (248.47, 119.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (216, 405, 4141)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11991)
[M::mem_pestat] mean and std.dev: (1355.46, 1590.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 15916)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 239, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (282.22, 89.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700382 reads in 174.289 CPU sec, 5.526 real sec
sorting block 53, #reads = 1087669 mem = 536884153 peak = 1007878144
[M::mem_process_seqs] Processed 699342 reads in 109.069 CPU sec, 3.437 real sec
[M::process] read 699604 sequences (100000020 bp) in 2.219 CPU sec, 2.233 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 291425, 4, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 157, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (238.07, 138.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (248.77, 120.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (330, 330, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (198, 528)
[M::mem_pestat] mean and std.dev: (336.29, 48.08)
[M::mem_pestat] low and high boundaries for proper pairs: (132, 594)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 54, #reads = 1088831 mem = 536879688 peak = 1044115456
[M::process] read 701504 sequences (100000025 bp) in 2.815 CPU sec, 2.987 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291542, 2, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 231, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715)
[M::mem_pestat] mean and std.dev: (226.78, 88.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 906)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (246.37, 118.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (328, 790, 888)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2008)
[M::mem_pestat] mean and std.dev: (533.09, 293.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2568)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 708470 sequences (100000128 bp) in 1.600 CPU sec, 1.597 real sec...
[M::mem_process_seqs] Processed 699214 reads in 172.098 CPU sec, 5.472 real sec
[M::mem_process_seqs] Processed 699604 reads in 110.525 CPU sec, 3.500 real sec
sorting block 55, #reads = 1088763 mem = 536877529 peak = 1044115456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292184, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (242.48, 117.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704726 sequences (100000182 bp) in 3.333 CPU sec, 3.555 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 294133, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 202, 292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586)
[M::mem_pestat] mean and std.dev: (226.95, 108.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 733)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (924, 924, 1094)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (584, 1434)
[M::mem_pestat] mean and std.dev: (1036.12, 152.55)
[M::mem_pestat] low and high boundaries for proper pairs: (414, 1646)
[M::mem_pestat] skip orientation RR
[M::process] read 704268 sequences (100000301 bp) in 1.750 CPU sec, 1.749 real sec...
[M::mem_process_seqs] Processed 701504 reads in 183.694 CPU sec, 5.839 real sec
[M::mem_process_seqs] Processed 708470 reads in 119.437 CPU sec, 3.793 real sec
sorting block 56, #reads = 1087168 mem = 536877764 peak = 1044115456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 293109, 6, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 334, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (260.30, 85.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 210, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (235.57, 113.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 702374 sequences (100000264 bp) in 2.961 CPU sec, 3.114 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 293178, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (237.40, 114.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 782)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 215, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1167)
[M::mem_pestat] mean and std.dev: (316.33, 304.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1535)
[M::mem_pestat] skip orientation RR
[M::process] read 701432 sequences (100000181 bp) in 1.660 CPU sec, 1.665 real sec...
[M::mem_process_seqs] Processed 704726 reads in 175.715 CPU sec, 5.587 real sec
sorting block 57, #reads = 1088344 mem = 536880451 peak = 1044115456
[M::mem_process_seqs] Processed 704268 reads in 111.273 CPU sec, 3.528 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292559, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.26, 117.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 58, #reads = 1091433 mem = 536879224 peak = 1044115456
[M::process] read 702862 sequences (100000075 bp) in 2.901 CPU sec, 3.078 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291788, 7, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (244.54, 118.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702530 sequences (100000028 bp) in 1.990 CPU sec, 1.988 real sec...
[M::mem_process_seqs] Processed 702374 reads in 172.370 CPU sec, 5.469 real sec
[M::mem_process_seqs] Processed 701432 reads in 108.205 CPU sec, 3.418 real sec
sorting block 59, #reads = 1091064 mem = 536878034 peak = 1044115456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 292937, 5, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 267, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (244.67, 115.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 731)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (239.89, 115.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (288, 340, 3974)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11346)
[M::mem_pestat] mean and std.dev: (1582.50, 1826.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 15032)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 701886 sequences (100000181 bp) in 2.907 CPU sec, 3.080 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292508, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (240.51, 116.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701776 sequences (100000222 bp) in 1.590 CPU sec, 1.591 real sec...
[M::mem_process_seqs] Processed 702862 reads in 159.804 CPU sec, 5.075 real sec
[M::mem_process_seqs] Processed 702530 reads in 96.579 CPU sec, 3.055 real sec
sorting block 60, #reads = 1093575 mem = 536880224 peak = 1044115456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292434, 11, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.25, 116.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (334, 780, 983)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2281)
[M::mem_pestat] mean and std.dev: (576.80, 341.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2930)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 702738 sequences (100000113 bp) in 2.771 CPU sec, 2.930 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292579, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (243.44, 117.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701886 reads in 152.062 CPU sec, 4.835 real sec
[M::process] read 699714 sequences (100000231 bp) in 1.720 CPU sec, 1.742 real sec...
[M::mem_process_seqs] Processed 701776 reads in 98.636 CPU sec, 3.115 real sec
sorting block 61, #reads = 1093372 mem = 536880966 peak = 1044115456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292513, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (240.32, 116.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698780 sequences (100000118 bp) in 3.078 CPU sec, 3.254 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291494, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.59, 119.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702738 reads in 176.200 CPU sec, 5.606 real sec
sorting block 62, #reads = 1091763 mem = 536883781 peak = 1044115456
[M::process] read 698672 sequences (100000021 bp) in 2.072 CPU sec, 2.096 real sec...
[M::mem_process_seqs] Processed 699714 reads in 108.365 CPU sec, 3.417 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291191, 7, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (107, 258, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560)
[M::mem_pestat] mean and std.dev: (211.20, 91.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 711)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.33, 121.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 63, #reads = 1087111 mem = 536880277 peak = 1044115456
[M::process] read 698472 sequences (100000153 bp) in 2.639 CPU sec, 2.812 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291257, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (250.78, 122.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698780 reads in 163.992 CPU sec, 5.223 real sec
[M::process] read 698160 sequences (100000044 bp) in 1.630 CPU sec, 1.627 real sec...
[M::mem_process_seqs] Processed 698672 reads in 101.944 CPU sec, 3.225 real sec
sorting block 64, #reads = 1091885 mem = 536878861 peak = 1044115456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291562, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 270, 1317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3621)
[M::mem_pestat] mean and std.dev: (747.30, 1040.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4911)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (251.21, 122.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 702726 sequences (100000030 bp) in 2.644 CPU sec, 2.795 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290927, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.82, 121.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698472 reads in 150.662 CPU sec, 4.792 real sec
[M::mem_process_seqs] Processed 698160 reads in 95.882 CPU sec, 3.019 real sec
[M::process] read 703048 sequences (100000289 bp) in 1.788 CPU sec, 1.818 real sec...
sorting block 65, #reads = 1091519 mem = 536877954 peak = 1044115456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 292116, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626)
[M::mem_pestat] mean and std.dev: (239.71, 116.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 784)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 209, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (238.45, 104.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RR
[M::process] read 702530 sequences (100000165 bp) in 2.559 CPU sec, 2.713 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292570, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (240.59, 117.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700900 sequences (100000025 bp) in 1.550 CPU sec, 1.553 real sec...
[M::mem_process_seqs] Processed 702726 reads in 153.396 CPU sec, 4.877 real sec
sorting block 66, #reads = 1088946 mem = 536879787 peak = 1044115456
[M::mem_process_seqs] Processed 703048 reads in 91.901 CPU sec, 2.904 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292250, 6, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (224, 543, 970)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2462)
[M::mem_pestat] mean and std.dev: (548.78, 329.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3208)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (242.38, 118.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 804)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 67, #reads = 1092881 mem = 536879702 peak = 1044115456
[M::process] read 699596 sequences (100000037 bp) in 2.747 CPU sec, 2.902 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291433, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (246.96, 120.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702530 reads in 139.897 CPU sec, 4.451 real sec
[M::mem_process_seqs] Processed 700900 reads in 87.408 CPU sec, 2.757 real sec
[M::process] read 699696 sequences (100000293 bp) in 1.810 CPU sec, 1.876 real sec...
sorting block 68, #reads = 1095119 mem = 536878419 peak = 1044115456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291531, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (250.37, 122.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 351, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (296.88, 81.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 757)
[M::mem_pestat] skip orientation RR
[M::process] read 701370 sequences (100000140 bp) in 2.208 CPU sec, 2.352 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291879, 3, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (217, 241, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (258.38, 100.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (251.08, 123.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 247, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (257.33, 122.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 857)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699596 reads in 136.634 CPU sec, 4.368 real sec
[M::mem_process_seqs] Processed 699696 reads in 85.696 CPU sec, 2.705 real sec
[M::process] read 701892 sequences (100000099 bp) in 2.006 CPU sec, 2.097 real sec...
sorting block 69, #reads = 1094527 mem = 536879294 peak = 1044115456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292513, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (245.55, 120.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (344, 381, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (236, 506)
[M::mem_pestat] mean and std.dev: (355.12, 48.01)
[M::mem_pestat] low and high boundaries for proper pairs: (163, 560)
[M::mem_pestat] skip orientation RR
[M::process] read 700824 sequences (100000030 bp) in 2.481 CPU sec, 2.619 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291974, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.64, 117.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701370 reads in 139.253 CPU sec, 4.444 real sec
[M::mem_process_seqs] Processed 701892 reads in 88.089 CPU sec, 2.785 real sec
sorting block 70, #reads = 1094465 mem = 536879691 peak = 1044115456
[M::process] read 699476 sequences (100000092 bp) in 2.036 CPU sec, 2.153 real sec...
[M::process] read 698846 sequences (100000006 bp) in 2.319 CPU sec, 2.494 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291522, 9, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (249.61, 122.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699476 reads in 77.793 CPU sec, 2.453 real sec
sorting block 71, #reads = 1095567 mem = 536879806 peak = 1044115456
[M::process] read 699050 sequences (100000099 bp) in 1.920 CPU sec, 1.994 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292135, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (246.94, 121.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291281, 2, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (213, 213, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (230.92, 89.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 721)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.83, 122.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 700824 reads in 216.474 CPU sec, 6.877 real sec
[M::mem_process_seqs] Processed 698846 reads in 99.917 CPU sec, 3.159 real sec
sorting block 72, #reads = 1093771 mem = 536881270 peak = 1044115456
[M::process] read 699220 sequences (100000274 bp) in 2.182 CPU sec, 2.282 real sec...
[M::process] read 698934 sequences (100000078 bp) in 2.212 CPU sec, 2.361 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291202, 1, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 322, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (273.56, 104.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (247.50, 119.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699220 reads in 80.477 CPU sec, 2.534 real sec
sorting block 73, #reads = 1093368 mem = 536877956 peak = 1044115456
[M::process] read 699100 sequences (100000220 bp) in 1.750 CPU sec, 1.805 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291238, 7, 17)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.26, 120.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 227, 563)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1361)
[M::mem_pestat] mean and std.dev: (357.06, 333.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1760)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290978, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.35, 120.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699050 reads in 223.703 CPU sec, 7.118 real sec
[M::mem_process_seqs] Processed 698934 reads in 103.073 CPU sec, 3.262 real sec
[M::process] read 699034 sequences (100000299 bp) in 2.217 CPU sec, 2.303 real sec...
sorting block 74, #reads = 1090973 mem = 536878398 peak = 1050001408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291241, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (246.34, 119.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699040 sequences (100000013 bp) in 2.594 CPU sec, 2.723 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290707, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 220, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (246.33, 119.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (108, 159, 276)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (177.22, 96.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 780)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699100 reads in 144.308 CPU sec, 4.595 real sec
[M::mem_process_seqs] Processed 699034 reads in 91.251 CPU sec, 2.878 real sec
sorting block 75, #reads = 1091161 mem = 536879302 peak = 1050001408
[M::process] read 706574 sequences (100000163 bp) in 1.965 CPU sec, 2.034 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290814, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (245.79, 118.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 804)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 76, #reads = 1091186 mem = 536878482 peak = 1050001408
[M::process] read 703634 sequences (100000004 bp) in 2.570 CPU sec, 2.702 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 293768, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 206, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598)
[M::mem_pestat] mean and std.dev: (230.89, 111.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 748)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702234 sequences (100000277 bp) in 1.500 CPU sec, 1.496 real sec...
[M::mem_process_seqs] Processed 699040 reads in 157.079 CPU sec, 4.993 real sec
[M::mem_process_seqs] Processed 706574 reads in 98.428 CPU sec, 3.115 real sec
sorting block 77, #reads = 1085624 mem = 536879144 peak = 1050001408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292465, 3, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (120, 146, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546)
[M::mem_pestat] mean and std.dev: (189.10, 98.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 688)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 211, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (237.83, 115.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 701458 sequences (100000277 bp) in 2.608 CPU sec, 2.739 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 293134, 2, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 201, 229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435)
[M::mem_pestat] mean and std.dev: (175.78, 63.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 538)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (244.02, 120.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 700142 sequences (100000174 bp) in 1.510 CPU sec, 1.514 real sec...
[M::mem_process_seqs] Processed 703634 reads in 145.675 CPU sec, 4.622 real sec
[M::mem_process_seqs] Processed 702234 reads in 90.651 CPU sec, 2.867 real sec
sorting block 78, #reads = 1091940 mem = 536880439 peak = 1050001408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291862, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (246.27, 121.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699720 sequences (100000257 bp) in 2.483 CPU sec, 2.601 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292195, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 223, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (251.15, 124.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699704 sequences (100000185 bp) in 1.500 CPU sec, 1.503 real sec...
[M::mem_process_seqs] Processed 701458 reads in 142.005 CPU sec, 4.507 real sec
sorting block 79, #reads = 1092325 mem = 536882140 peak = 1050001408
[M::mem_process_seqs] Processed 700142 reads in 88.669 CPU sec, 2.800 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291756, 7, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (250.43, 122.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 212, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068)
[M::mem_pestat] mean and std.dev: (321.46, 249.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1371)
[M::mem_pestat] skip orientation RR
sorting block 80, #reads = 1091840 mem = 536886413 peak = 1050001408
[M::process] read 698458 sequences (100000075 bp) in 2.642 CPU sec, 2.811 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291540, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (248.47, 120.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699720 reads in 138.636 CPU sec, 4.405 real sec
[M::mem_process_seqs] Processed 699704 reads in 87.261 CPU sec, 2.752 real sec
[M::process] read 697938 sequences (100000204 bp) in 1.792 CPU sec, 1.841 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291193, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (251.28, 121.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 227, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (265.10, 119.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 881)
[M::mem_pestat] skip orientation RR
sorting block 81, #reads = 1093369 mem = 536878606 peak = 1050001408
[M::process] read 697484 sequences (100000000 bp) in 2.548 CPU sec, 2.693 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 290662, 5, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (295, 1464, 2621)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7273)
[M::mem_pestat] mean and std.dev: (1613.54, 1420.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9599)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (251.50, 120.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698458 reads in 140.460 CPU sec, 4.470 real sec
[M::mem_process_seqs] Processed 697938 reads in 89.943 CPU sec, 2.832 real sec
[M::process] read 697182 sequences (100000252 bp) in 1.774 CPU sec, 1.827 real sec...
sorting block 82, #reads = 1090618 mem = 536884294 peak = 1050001408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290149, 10, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (251.06, 119.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (55, 928, 1459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4267)
[M::mem_pestat] mean and std.dev: (870.11, 916.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5671)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 696532 sequences (100000265 bp) in 2.520 CPU sec, 2.689 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290619, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (249.96, 118.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697484 reads in 140.167 CPU sec, 4.465 real sec
[M::mem_process_seqs] Processed 697182 reads in 87.567 CPU sec, 2.761 real sec
[M::process] read 696814 sequences (100000243 bp) in 1.871 CPU sec, 1.940 real sec...
sorting block 83, #reads = 1089803 mem = 536879825 peak = 1050001408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290498, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.57, 118.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696840 sequences (100000211 bp) in 2.552 CPU sec, 2.680 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290022, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 227, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (249.65, 117.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 812)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696532 reads in 136.732 CPU sec, 4.357 real sec
[M::mem_process_seqs] Processed 696814 reads in 86.229 CPU sec, 2.723 real sec
sorting block 84, #reads = 1090689 mem = 536880644 peak = 1050001408
[M::process] read 696346 sequences (100000023 bp) in 2.062 CPU sec, 2.164 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290383, 8, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 226, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (249.83, 118.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 85, #reads = 1091527 mem = 536883702 peak = 1050001408
[M::process] read 696720 sequences (100000156 bp) in 2.428 CPU sec, 2.546 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290142, 4, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (253, 304, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (308.91, 126.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.45, 118.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696840 reads in 136.520 CPU sec, 4.357 real sec
[M::mem_process_seqs] Processed 696346 reads in 85.595 CPU sec, 2.704 real sec
[M::process] read 696738 sequences (100000017 bp) in 2.127 CPU sec, 2.237 real sec...
[M::process] read 10766 sequences (1537445 bp) in 0.447 CPU sec, 0.449 real sec...
sorting block 86, #reads = 1091937 mem = 536879700 peak = 1050001408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290407, 5, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.35, 118.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 740, 1724)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4730)
[M::mem_pestat] mean and std.dev: (1032.17, 1009.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6233)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290234, 7, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (252.37, 120.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 249, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699)
[M::mem_pestat] mean and std.dev: (217.58, 81.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696720 reads in 134.629 CPU sec, 4.367 real sec
[M::mem_process_seqs] Processed 696738 reads in 84.926 CPU sec, 2.752 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 4465, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (242.98, 112.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 10766 reads in 1.371 CPU sec, 0.051 real sec
sorting block 87, #reads = 1091697 mem = 536880662 peak = 1050001408
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 96453350
overall: 69995393024 mem 12320.767 user 478.042 sys 487.025 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1047_JG-T1-TDNA-1_A23MHWWLT4_1.L001\tPU:L001\tSM:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tLB:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0001.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0001.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 487.062 sec; CPU: 12798.812 sec
sorting block 88, #reads = 804449 mem = 396254942 peak = 1131229184
uniq header count 1
merging 89 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 5149122124
output reads: 96943476
bam_mem_sort: 89 calls 42.738 user 1.017 sys 43.961 real
bam_write: 89 calls 19.986 user 1.127 sys 21.044 real
execute: 1 calls 19.803 user 1.324 sys 514.987 real
merge_files: 1 calls 19.791 user 1.294 sys 27.052 real
parse_chunk: 1420 calls 52.272 user 13.859 sys 67.227 real
read_chunk: 3835 calls 35.687 user 36.181 sys 368.765 real
sort_block: 1 calls 27.247 user 35.486 sys 356.215 real
write_chunk: 2413 calls 19.477 user 1.034 sys 26.246 real
overall: 3062038528 mem 1081.667 user 61.809 sys 515.011 real
ls: cannot access '*.cram': No such file or directory