cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_7OW-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1947.888 CPU sec, 68.045 real sec [M::process] read 684910 sequences (100000066 bp) in 0.967 CPU sec, 0.975 real sec... [M::process] read 680762 sequences (100000121 bp) in 1.230 CPU sec, 1.224 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 283433, 7, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 234, 641) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1549) [M::mem_pestat] mean and std.dev: (307.46, 202.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2003) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 310, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (328.83, 160.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 220, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (238.08, 107.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1071) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681850 sequences (100000056 bp) in 1.548 CPU sec, 1.568 real sec... [M::process] read 681996 sequences (100000123 bp) in 1.480 CPU sec, 1.484 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 281940, 17, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 375, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950) [M::mem_pestat] mean and std.dev: (409.02, 233.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1345) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (220, 338, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955) [M::mem_pestat] mean and std.dev: (352.70, 165.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1200) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1013, 1842, 2677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6005) [M::mem_pestat] mean and std.dev: (1640.88, 946.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7669) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (263, 351, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (325.73, 100.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684910 reads in 170.712 CPU sec, 5.409 real sec [M::mem_process_seqs] Processed 680762 reads in 110.883 CPU sec, 3.541 real sec sorting block 0, #reads = 1078485 mem = 536879906 peak = 595730432 [M::process] read 684014 sequences (100000044 bp) in 2.087 CPU sec, 2.184 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 281882, 12, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (327, 662, 920) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2106) [M::mem_pestat] mean and std.dev: (476.95, 232.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 332, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953) [M::mem_pestat] mean and std.dev: (348.15, 166.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1199) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (796, 1153, 1183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (22, 1957) [M::mem_pestat] mean and std.dev: (901.82, 319.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2344) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 249, 677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1653) [M::mem_pestat] mean and std.dev: (373.35, 277.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2141) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684208 sequences (100000021 bp) in 1.678 CPU sec, 1.684 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 282246, 3, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 286, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (264.56, 114.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 330, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951) [M::mem_pestat] mean and std.dev: (346.37, 165.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1197) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 241, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (292.52, 130.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 283286, 6, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (271, 294, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (342.83, 181.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1067) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 316, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (335.70, 163.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 264, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950) [M::mem_pestat] mean and std.dev: (308.87, 160.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1201) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681850 reads in 242.614 CPU sec, 7.753 real sec [M::mem_process_seqs] Processed 681996 reads in 185.036 CPU sec, 5.938 real sec [M::mem_process_seqs] Processed 684014 reads in 203.477 CPU sec, 6.554 real sec sorting block 1, #reads = 1078110 mem = 536877715 peak = 724705280 [M::process] read 684232 sequences (100000017 bp) in 1.941 CPU sec, 2.054 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282554, 7, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 247, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (273.34, 114.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 318, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932) [M::mem_pestat] mean and std.dev: (336.15, 164.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1175) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 305, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (295.68, 122.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 2, #reads = 1079153 mem = 536883562 peak = 897785856 [M::process] read 684184 sequences (100000186 bp) in 2.116 CPU sec, 2.222 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 283319, 8, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 323, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (288.29, 110.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 315, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (333.80, 162.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1159) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (273, 481, 673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1473) [M::mem_pestat] mean and std.dev: (400.40, 225.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1873) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680278 sequences (100000174 bp) in 1.653 CPU sec, 1.661 real sec... [M::mem_process_seqs] Processed 684208 reads in 178.684 CPU sec, 5.699 real sec [M::mem_process_seqs] Processed 684232 reads in 116.804 CPU sec, 3.703 real sec sorting block 3, #reads = 1079990 mem = 536877691 peak = 897785856 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282916, 5, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 298, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (270.89, 112.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 315, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (334.45, 163.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1170) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1196) [M::mem_pestat] mean and std.dev: (272.78, 158.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1547) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681998 sequences (100000283 bp) in 2.080 CPU sec, 2.196 real sec... [M::process] read 684390 sequences (100000110 bp) in 1.809 CPU sec, 1.899 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 281698, 4, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 560, 571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1273) [M::mem_pestat] mean and std.dev: (448.73, 270.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (223, 345, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976) [M::mem_pestat] mean and std.dev: (358.30, 168.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1227) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 245, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (263.04, 116.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684184 reads in 170.944 CPU sec, 5.462 real sec [M::mem_process_seqs] Processed 680278 reads in 113.476 CPU sec, 3.643 real sec sorting block 4, #reads = 1080906 mem = 536883919 peak = 897785856 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 281513, 17, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 256, 1148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3124) [M::mem_pestat] mean and std.dev: (393.00, 362.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4112) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 331, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960) [M::mem_pestat] mean and std.dev: (348.38, 167.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1209) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (713, 713, 713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (713, 713) [M::mem_pestat] mean and std.dev: (713.00, 0.00) [M::mem_pestat] low and high boundaries for proper pairs: (713, 713) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 280, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (286.04, 104.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683466 sequences (100000031 bp) in 2.354 CPU sec, 2.608 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 282687, 4, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 325, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047) [M::mem_pestat] mean and std.dev: (339.27, 187.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1324) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 315, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (334.67, 163.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1167) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 232, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (243.61, 102.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683058 sequences (100000073 bp) in 1.657 CPU sec, 1.676 real sec... [M::mem_process_seqs] Processed 681998 reads in 176.318 CPU sec, 5.745 real sec [M::mem_process_seqs] Processed 684390 reads in 118.235 CPU sec, 3.847 real sec sorting block 5, #reads = 1078854 mem = 536882119 peak = 897785856 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282305, 7, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 197, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (199.04, 104.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 990) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 321, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (338.96, 164.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1182) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (253, 350, 612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1330) [M::mem_pestat] mean and std.dev: (343.69, 163.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1689) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683190 sequences (100000296 bp) in 2.570 CPU sec, 2.980 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282205, 1, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 321, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (269.72, 115.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 321, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936) [M::mem_pestat] mean and std.dev: (339.47, 163.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1179) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 324, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952) [M::mem_pestat] mean and std.dev: (376.17, 215.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1237) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683384 sequences (100000215 bp) in 1.758 CPU sec, 1.804 real sec... [M::mem_process_seqs] Processed 683466 reads in 178.389 CPU sec, 5.776 real sec sorting block 6, #reads = 1079631 mem = 536878742 peak = 897785856 [M::mem_process_seqs] Processed 683058 reads in 117.892 CPU sec, 3.785 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 282923, 4, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 217, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (238.48, 104.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 319, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (338.67, 164.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1182) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 207, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (222.22, 112.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 7, #reads = 1080162 mem = 536880550 peak = 897785856 [M::process] read 681276 sequences (100000188 bp) in 2.341 CPU sec, 2.550 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 282703, 3, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 286, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (252.56, 97.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 317, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (336.20, 162.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1169) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (275, 341, 632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1346) [M::mem_pestat] mean and std.dev: (424.98, 222.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1703) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681776 sequences (100000039 bp) in 1.922 CPU sec, 1.954 real sec... [M::mem_process_seqs] Processed 683190 reads in 177.236 CPU sec, 5.710 real sec [M::mem_process_seqs] Processed 683384 reads in 116.695 CPU sec, 3.746 real sec sorting block 8, #reads = 1080572 mem = 536877286 peak = 897785856 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 281427, 9, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 324, 541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1175) [M::mem_pestat] mean and std.dev: (377.59, 236.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 329, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (346.45, 164.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1190) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 195, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (255.65, 132.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683486 sequences (100000099 bp) in 2.689 CPU sec, 2.983 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 282082, 5, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 248, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027) [M::mem_pestat] mean and std.dev: (291.95, 189.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1315) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 326, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (343.18, 163.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1176) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 269, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1131) [M::mem_pestat] mean and std.dev: (323.21, 238.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1455) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681276 reads in 177.926 CPU sec, 5.790 real sec [M::process] read 682344 sequences (100000161 bp) in 1.938 CPU sec, 2.009 real sec... [M::mem_process_seqs] Processed 681776 reads in 118.862 CPU sec, 3.856 real sec sorting block 9, #reads = 1079211 mem = 536879304 peak = 897785856 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 282156, 3, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 292, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (288.36, 136.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 313, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (332.92, 160.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 246, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (288.29, 150.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681500 sequences (100000134 bp) in 2.419 CPU sec, 2.846 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282331, 9, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 238, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (239.69, 130.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 322, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (340.45, 163.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1170) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 282, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (276.97, 95.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683486 reads in 178.745 CPU sec, 5.831 real sec [M::process] read 682632 sequences (100000039 bp) in 1.735 CPU sec, 1.811 real sec... sorting block 10, #reads = 1077888 mem = 536878049 peak = 897785856 [M::mem_process_seqs] Processed 682344 reads in 117.975 CPU sec, 3.798 real sec [M::process] read 682302 sequences (100000228 bp) in 2.657 CPU sec, 2.954 real sec... sorting block 11, #reads = 1079163 mem = 536879311 peak = 897785856 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 281589, 11, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 268, 552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1296) [M::mem_pestat] mean and std.dev: (319.02, 175.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 317, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (336.07, 161.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1155) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1090, 1090, 1303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (664, 1729) [M::mem_pestat] mean and std.dev: (1186.10, 208.98) [M::mem_pestat] low and high boundaries for proper pairs: (350, 2022) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 277, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (318.12, 153.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1186) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682632 reads in 101.536 CPU sec, 3.241 real sec [M::process] read 682412 sequences (100000201 bp) in 1.891 CPU sec, 1.958 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 281442, 6, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (234, 399, 778) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1866) [M::mem_pestat] mean and std.dev: (516.95, 386.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2410) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 326, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (342.89, 162.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1165) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 268, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (259.88, 142.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 281715, 10, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (267, 393, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804) [M::mem_pestat] mean and std.dev: (373.08, 144.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 317, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (334.26, 158.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1136) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (694, 694, 2192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5188) [M::mem_pestat] mean and std.dev: (799.25, 545.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6686) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 282, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810) [M::mem_pestat] mean and std.dev: (283.15, 151.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681500 reads in 276.918 CPU sec, 8.990 real sec [M::mem_process_seqs] Processed 682302 reads in 137.992 CPU sec, 4.469 real sec [M::process] read 680386 sequences (100000105 bp) in 2.434 CPU sec, 2.582 real sec... sorting block 12, #reads = 1077618 mem = 536878300 peak = 897785856 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281675, 4, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 337, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022) [M::mem_pestat] mean and std.dev: (316.89, 141.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1290) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 320, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (337.78, 161.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 319, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (303.27, 110.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681588 sequences (100000166 bp) in 2.507 CPU sec, 2.868 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 281307, 11, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 314, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1347) [M::mem_pestat] mean and std.dev: (334.77, 213.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1741) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (218, 331, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (346.74, 162.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1166) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (178, 566, 1629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4531) [M::mem_pestat] mean and std.dev: (886.27, 800.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5982) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 354, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1154) [M::mem_pestat] mean and std.dev: (364.42, 215.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1483) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682412 reads in 176.131 CPU sec, 5.727 real sec sorting block 13, #reads = 1078649 mem = 536876833 peak = 897785856 [M::mem_process_seqs] Processed 680386 reads in 117.608 CPU sec, 3.809 real sec [M::process] read 682530 sequences (100000096 bp) in 2.134 CPU sec, 2.321 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 281746, 10, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 346, 544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1276) [M::mem_pestat] mean and std.dev: (344.58, 239.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 323, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (339.75, 160.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (165, 275, 1278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3504) [M::mem_pestat] mean and std.dev: (623.10, 535.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4617) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 250, 607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1419) [M::mem_pestat] mean and std.dev: (419.53, 404.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2039) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 14, #reads = 1078181 mem = 536878601 peak = 914403328 [M::process] read 682786 sequences (100000006 bp) in 2.634 CPU sec, 2.961 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 281956, 8, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 314, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1084) [M::mem_pestat] mean and std.dev: (313.89, 178.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 315, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (334.41, 160.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1143) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 298, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (309.19, 156.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1150) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681588 reads in 176.372 CPU sec, 6.072 real sec [M::mem_process_seqs] Processed 682530 reads in 118.741 CPU sec, 4.156 real sec [M::process] read 680972 sequences (100000207 bp) in 2.317 CPU sec, 2.627 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 281816, 6, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (283, 407, 563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1123) [M::mem_pestat] mean and std.dev: (362.68, 174.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 314, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (332.79, 159.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1138) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 327, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1082) [M::mem_pestat] mean and std.dev: (326.43, 245.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1392) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 15, #reads = 1077791 mem = 536878447 peak = 914403328 [M::process] read 682436 sequences (100000173 bp) in 2.331 CPU sec, 2.670 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281215, 8, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 256, 515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1177) [M::mem_pestat] mean and std.dev: (321.69, 191.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 326, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (342.51, 161.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1151) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (311, 402, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (383.18, 135.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1083) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682786 reads in 177.151 CPU sec, 5.913 real sec [M::mem_process_seqs] Processed 680972 reads in 117.734 CPU sec, 3.945 real sec [M::process] read 682128 sequences (100000032 bp) in 2.304 CPU sec, 2.515 real sec... sorting block 16, #reads = 1078626 mem = 536878805 peak = 914403328 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 281436, 5, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (273, 323, 735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1659) [M::mem_pestat] mean and std.dev: (433.59, 286.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2121) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 319, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (337.63, 161.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 238, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (270.85, 155.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 997) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682106 sequences (100000112 bp) in 2.374 CPU sec, 2.794 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 281438, 7, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 224, 616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1480) [M::mem_pestat] mean and std.dev: (292.97, 200.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1912) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 321, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (340.20, 163.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1169) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 240, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015) [M::mem_pestat] mean and std.dev: (323.48, 248.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1318) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682436 reads in 179.704 CPU sec, 6.040 real sec [M::mem_process_seqs] Processed 682128 reads in 120.147 CPU sec, 4.025 real sec sorting block 17, #reads = 1078965 mem = 536878138 peak = 914403328 [M::process] read 680988 sequences (100000268 bp) in 2.367 CPU sec, 2.669 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282357, 2, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 323, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (275.96, 101.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 323, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (341.79, 164.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 248, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (258.52, 86.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683296 sequences (100000235 bp) in 2.287 CPU sec, 2.584 real sec... sorting block 18, #reads = 1077933 mem = 536881873 peak = 914403328 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 281710, 12, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 374, 1808) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5004) [M::mem_pestat] mean and std.dev: (776.23, 800.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 332, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954) [M::mem_pestat] mean and std.dev: (349.60, 167.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1200) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (66, 266, 1153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3327) [M::mem_pestat] mean and std.dev: (383.82, 414.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4414) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 241, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (237.09, 89.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682106 reads in 177.415 CPU sec, 5.988 real sec [M::mem_process_seqs] Processed 680988 reads in 118.453 CPU sec, 3.957 real sec [M::process] read 683590 sequences (100000041 bp) in 2.234 CPU sec, 2.539 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 282996, 14, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (294, 380, 640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1332) [M::mem_pestat] mean and std.dev: (493.16, 347.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1881) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 320, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (338.13, 163.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1167) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (605, 976, 1600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3590) [M::mem_pestat] mean and std.dev: (1161.21, 681.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4585) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 313, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (315.26, 143.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 19, #reads = 1078154 mem = 536876784 peak = 914403328 [M::process] read 683102 sequences (100000178 bp) in 2.366 CPU sec, 2.744 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 282112, 5, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 277, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958) [M::mem_pestat] mean and std.dev: (288.44, 121.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1210) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 317, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (335.12, 161.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1150) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 304, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (319.39, 164.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683296 reads in 176.223 CPU sec, 5.985 real sec [M::mem_process_seqs] Processed 683590 reads in 116.576 CPU sec, 3.923 real sec [M::process] read 682252 sequences (100000108 bp) in 2.276 CPU sec, 2.444 real sec... sorting block 20, #reads = 1078014 mem = 536881489 peak = 914403328 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 281749, 4, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 205, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (252.88, 105.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 322, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923) [M::mem_pestat] mean and std.dev: (338.78, 162.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 321, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016) [M::mem_pestat] mean and std.dev: (355.98, 218.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1286) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683342 sequences (100000158 bp) in 2.459 CPU sec, 2.779 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 282733, 10, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 222, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029) [M::mem_pestat] mean and std.dev: (238.39, 179.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1329) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 331, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943) [M::mem_pestat] mean and std.dev: (346.25, 165.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1186) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (6815, 6815, 6815) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (6815, 6815) [M::mem_pestat] mean and std.dev: (6815.00, 0.00) [M::mem_pestat] low and high boundaries for proper pairs: (6815, 6815) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 232, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (260.57, 155.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683102 reads in 178.713 CPU sec, 5.976 real sec [M::mem_process_seqs] Processed 682252 reads in 118.452 CPU sec, 3.913 real sec sorting block 21, #reads = 1079705 mem = 536883486 peak = 914403328 [M::process] read 683146 sequences (100000184 bp) in 2.680 CPU sec, 2.932 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 282575, 17, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 274, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (308.22, 200.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 322, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (339.54, 164.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1168) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (703, 703, 1453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2953) [M::mem_pestat] mean and std.dev: (897.75, 681.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3703) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 259, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (239.54, 73.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682894 sequences (100000071 bp) in 2.547 CPU sec, 2.893 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282907, 4, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 317, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1061) [M::mem_pestat] mean and std.dev: (364.22, 228.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1347) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 325, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (342.54, 166.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1192) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 298, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995) [M::mem_pestat] mean and std.dev: (293.84, 138.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1256) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683342 reads in 178.941 CPU sec, 5.984 real sec [M::mem_process_seqs] Processed 683146 reads in 117.957 CPU sec, 3.939 real sec sorting block 22, #reads = 1080866 mem = 536880029 peak = 914403328 [M::process] read 683120 sequences (100000200 bp) in 2.498 CPU sec, 2.798 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 282551, 4, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 270, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (278.85, 126.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1019) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 327, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942) [M::mem_pestat] mean and std.dev: (343.34, 165.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1186) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 292, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (276.93, 120.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 23, #reads = 1081705 mem = 536878086 peak = 914403328 [M::process] read 682978 sequences (100000054 bp) in 2.468 CPU sec, 2.836 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282860, 4, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 358, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076) [M::mem_pestat] mean and std.dev: (350.42, 153.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1351) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 323, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931) [M::mem_pestat] mean and std.dev: (339.42, 163.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 243, 590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1388) [M::mem_pestat] mean and std.dev: (292.45, 157.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1787) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682894 reads in 180.435 CPU sec, 6.108 real sec [M::mem_process_seqs] Processed 683120 reads in 120.640 CPU sec, 4.118 real sec [M::process] read 681214 sequences (100000107 bp) in 2.539 CPU sec, 3.015 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282023, 15, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 331, 712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1724) [M::mem_pestat] mean and std.dev: (430.79, 322.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2230) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 322, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931) [M::mem_pestat] mean and std.dev: (339.98, 164.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (374, 476, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (84, 809) [M::mem_pestat] mean and std.dev: (452.57, 123.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 297, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (271.98, 92.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682978 reads in 99.397 CPU sec, 3.679 real sec sorting block 24, #reads = 1080001 mem = 536877472 peak = 914403328 [M::process] read 682630 sequences (100000290 bp) in 2.431 CPU sec, 2.876 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 281931, 6, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 216, 549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1375) [M::mem_pestat] mean and std.dev: (322.09, 224.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1788) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (217, 335, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (349.60, 165.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1189) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (238, 344, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (306.22, 106.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681214 reads in 98.901 CPU sec, 3.569 real sec [M::process] read 683148 sequences (100000218 bp) in 2.714 CPU sec, 3.097 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282292, 7, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 204, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042) [M::mem_pestat] mean and std.dev: (286.36, 229.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1348) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 325, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936) [M::mem_pestat] mean and std.dev: (341.76, 164.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1178) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (262, 344, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (324.76, 159.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 25, #reads = 1080899 mem = 536881186 peak = 914403328 [M::mem_process_seqs] Processed 682630 reads in 98.329 CPU sec, 3.544 real sec [M::process] read 683040 sequences (100000191 bp) in 2.356 CPU sec, 2.693 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 282100, 11, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 300, 580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1310) [M::mem_pestat] mean and std.dev: (347.88, 203.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 323, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (340.04, 164.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1176) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (252, 952, 1686) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4554) [M::mem_pestat] mean and std.dev: (1007.00, 848.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5988) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 328, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (295.24, 125.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1100) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683148 reads in 104.000 CPU sec, 3.721 real sec sorting block 26, #reads = 1080440 mem = 536879167 peak = 914403328 [M::process] read 683044 sequences (100000115 bp) in 2.454 CPU sec, 2.956 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 282889, 6, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 254, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (229.24, 70.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 323, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937) [M::mem_pestat] mean and std.dev: (340.77, 164.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1180) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 275, 738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1880) [M::mem_pestat] mean and std.dev: (355.31, 333.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2451) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683040 reads in 96.722 CPU sec, 3.565 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 283038, 3, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 558, 1253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3311) [M::mem_pestat] mean and std.dev: (620.00, 478.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4340) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 322, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931) [M::mem_pestat] mean and std.dev: (340.11, 164.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 296, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (299.83, 173.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682736 sequences (100000104 bp) in 2.514 CPU sec, 2.949 real sec... [M::mem_process_seqs] Processed 683044 reads in 101.848 CPU sec, 3.613 real sec sorting block 27, #reads = 1080744 mem = 536880311 peak = 914403328 [M::process] read 682060 sequences (100000268 bp) in 2.558 CPU sec, 2.974 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 282646, 4, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 184, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (214.80, 86.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 324, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (341.38, 164.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1177) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 250, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (246.94, 83.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682736 reads in 102.245 CPU sec, 3.651 real sec sorting block 28, #reads = 1080937 mem = 536877807 peak = 914403328 [M::process] read 680158 sequences (100000109 bp) in 2.322 CPU sec, 2.757 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 282387, 8, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 274, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (278.34, 131.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 325, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (342.38, 164.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1168) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 331, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002) [M::mem_pestat] mean and std.dev: (321.22, 219.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1288) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682060 reads in 99.295 CPU sec, 3.706 real sec [M::process] read 681444 sequences (100000244 bp) in 2.803 CPU sec, 3.156 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 281540, 6, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 233, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (254.28, 78.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (222, 338, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942) [M::mem_pestat] mean and std.dev: (352.64, 164.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1182) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 309, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (260.21, 90.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680158 reads in 103.828 CPU sec, 3.635 real sec sorting block 29, #reads = 1080056 mem = 536880405 peak = 914403328 [M::process] read 682626 sequences (100000225 bp) in 2.317 CPU sec, 2.677 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 282006, 8, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 322, 1195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3197) [M::mem_pestat] mean and std.dev: (659.89, 656.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4198) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 329, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937) [M::mem_pestat] mean and std.dev: (345.83, 165.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1178) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 342, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974) [M::mem_pestat] mean and std.dev: (299.31, 136.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1243) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681444 reads in 99.755 CPU sec, 3.753 real sec [M::process] read 682296 sequences (100000114 bp) in 2.480 CPU sec, 2.902 real sec... sorting block 30, #reads = 1079793 mem = 536878022 peak = 914403328 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 282835, 12, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 299, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (271.33, 116.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 318, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (336.95, 162.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1138, 1238, 1258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (898, 1498) [M::mem_pestat] mean and std.dev: (1190.00, 100.88) [M::mem_pestat] low and high boundaries for proper pairs: (778, 1618) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 236, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (247.90, 96.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682626 reads in 100.967 CPU sec, 3.654 real sec [M::process] read 682846 sequences (100000057 bp) in 2.277 CPU sec, 2.691 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282885, 6, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 202, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (241.34, 132.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 323, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (341.00, 164.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1170) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 259, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (267.97, 110.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682296 reads in 102.464 CPU sec, 3.685 real sec sorting block 31, #reads = 1079749 mem = 536880972 peak = 914403328 [M::process] read 682932 sequences (100000211 bp) in 2.409 CPU sec, 2.748 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282178, 4, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 231, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1257) [M::mem_pestat] mean and std.dev: (300.54, 212.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 320, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931) [M::mem_pestat] mean and std.dev: (339.80, 165.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 336, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1104) [M::mem_pestat] mean and std.dev: (346.35, 192.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1415) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682846 reads in 103.361 CPU sec, 3.829 real sec [M::process] read 682790 sequences (100000065 bp) in 2.738 CPU sec, 3.201 real sec... sorting block 32, #reads = 1080266 mem = 536881397 peak = 921661440 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282445, 8, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 194, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (195.65, 96.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 319, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (338.51, 165.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1170) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 294, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1263) [M::mem_pestat] mean and std.dev: (334.61, 233.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1631) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681676 sequences (100000121 bp) in 2.491 CPU sec, 2.840 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 282618, 2, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 270, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (240.02, 96.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 316, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (335.10, 161.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1143) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 392, 690) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1654) [M::mem_pestat] mean and std.dev: (443.38, 323.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2136) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682932 reads in 188.551 CPU sec, 6.668 real sec [M::mem_process_seqs] Processed 682790 reads in 122.197 CPU sec, 4.330 real sec sorting block 33, #reads = 1079154 mem = 536877724 peak = 921661440 [M::process] read 680404 sequences (100000198 bp) in 2.416 CPU sec, 2.799 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 281960, 11, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 199, 688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1752) [M::mem_pestat] mean and std.dev: (360.10, 335.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2284) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 323, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (340.81, 162.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (710, 1614, 9087) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 25841) [M::mem_pestat] mean and std.dev: (3199.91, 3642.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 34218) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 302, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037) [M::mem_pestat] mean and std.dev: (333.94, 155.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1319) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681676 reads in 103.369 CPU sec, 3.780 real sec [M::process] read 682626 sequences (100000103 bp) in 2.491 CPU sec, 3.037 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 281630, 14, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 368, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (346.90, 138.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1074) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (218, 332, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932) [M::mem_pestat] mean and std.dev: (348.13, 164.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1170) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (302, 922, 1650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4346) [M::mem_pestat] mean and std.dev: (827.23, 612.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5694) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 292, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032) [M::mem_pestat] mean and std.dev: (282.36, 153.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1324) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680404 reads in 100.837 CPU sec, 3.635 real sec sorting block 34, #reads = 1080815 mem = 536883261 peak = 921661440 [M::process] read 682548 sequences (100000152 bp) in 2.356 CPU sec, 3.016 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 281911, 10, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 332, 1071) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2829) [M::mem_pestat] mean and std.dev: (575.47, 435.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3708) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 316, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (335.88, 162.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 29, 33) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 75) [M::mem_pestat] mean and std.dev: (23.44, 15.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 96) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 210, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (236.08, 94.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682626 reads in 98.110 CPU sec, 3.652 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 283037, 7, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 248, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991) [M::mem_pestat] mean and std.dev: (276.32, 160.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1270) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 318, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (337.31, 162.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 334, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (340.91, 172.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 35, #reads = 1078095 mem = 536878800 peak = 921661440 [M::process] read 682522 sequences (100000068 bp) in 3.033 CPU sec, 3.361 real sec... [M::mem_process_seqs] Processed 682548 reads in 101.762 CPU sec, 3.755 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282182, 7, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 249, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (289.81, 142.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 989) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 318, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (336.68, 162.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1155) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 466, 1031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2611) [M::mem_pestat] mean and std.dev: (629.97, 543.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3401) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682716 sequences (100000012 bp) in 2.363 CPU sec, 2.781 real sec... [M::mem_process_seqs] Processed 682522 reads in 96.101 CPU sec, 3.466 real sec sorting block 36, #reads = 1078043 mem = 536876726 peak = 924672000 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 282353, 12, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 225, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (226.87, 90.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 317, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (335.64, 161.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (946, 946, 2316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5056) [M::mem_pestat] mean and std.dev: (1465.67, 726.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6426) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 307, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (337.33, 131.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1170) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682590 sequences (100000016 bp) in 2.642 CPU sec, 3.017 real sec... [M::mem_process_seqs] Processed 682716 reads in 99.946 CPU sec, 3.614 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282631, 6, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 325, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1063) [M::mem_pestat] mean and std.dev: (378.60, 232.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1348) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 318, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (337.39, 162.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (256, 404, 680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1528) [M::mem_pestat] mean and std.dev: (397.49, 208.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1952) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683040 sequences (100000249 bp) in 2.358 CPU sec, 2.753 real sec... sorting block 37, #reads = 1079391 mem = 536877055 peak = 931909632 [M::mem_process_seqs] Processed 682590 reads in 97.331 CPU sec, 3.567 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282390, 9, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (242, 327, 909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2243) [M::mem_pestat] mean and std.dev: (538.32, 461.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2910) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 320, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (339.77, 165.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1175) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 295, 976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2458) [M::mem_pestat] mean and std.dev: (509.41, 357.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3199) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682826 sequences (100000056 bp) in 2.884 CPU sec, 3.364 real sec... [M::mem_process_seqs] Processed 683040 reads in 101.139 CPU sec, 3.712 real sec sorting block 38, #reads = 1079180 mem = 536879253 peak = 931909632 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 282615, 3, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 294, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1099) [M::mem_pestat] mean and std.dev: (351.59, 222.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1395) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 317, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (337.55, 164.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1163) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (119, 276, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1211) [M::mem_pestat] mean and std.dev: (252.71, 155.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1575) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683142 sequences (100000208 bp) in 2.433 CPU sec, 3.003 real sec... [M::mem_process_seqs] Processed 682826 reads in 101.712 CPU sec, 3.679 real sec [M::process] read 684758 sequences (100000262 bp) in 2.446 CPU sec, 2.730 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282911, 8, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 234, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1099) [M::mem_pestat] mean and std.dev: (241.62, 145.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 319, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (338.24, 164.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1167) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 280, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965) [M::mem_pestat] mean and std.dev: (298.29, 147.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1220) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 39, #reads = 1078395 mem = 536879215 peak = 931909632 [M::mem_process_seqs] Processed 683142 reads in 102.002 CPU sec, 3.715 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 283183, 10, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 482, 1781) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4931) [M::mem_pestat] mean and std.dev: (709.67, 677.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 312, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (328.27, 156.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (109, 1014, 1855) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5347) [M::mem_pestat] mean and std.dev: (1046.30, 1034.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7093) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 320, 758) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1892) [M::mem_pestat] mean and std.dev: (446.41, 349.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2459) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684078 sequences (100000236 bp) in 2.593 CPU sec, 3.158 real sec... [M::mem_process_seqs] Processed 684758 reads in 103.631 CPU sec, 3.841 real sec sorting block 40, #reads = 1078449 mem = 536878173 peak = 931909632 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 282586, 2, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (280, 319, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (312.71, 116.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 314, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (329.82, 156.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (244, 375, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (308.38, 108.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684076 sequences (100000050 bp) in 2.729 CPU sec, 3.169 real sec... [M::mem_process_seqs] Processed 684078 reads in 105.084 CPU sec, 3.784 real sec [M::process] read 683710 sequences (100000160 bp) in 2.406 CPU sec, 2.707 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 283054, 4, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (290, 469, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971) [M::mem_pestat] mean and std.dev: (366.69, 141.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1198) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 314, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (330.24, 157.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 318, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (289.21, 128.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684076 reads in 100.563 CPU sec, 3.707 real sec sorting block 41, #reads = 1078250 mem = 536881512 peak = 931909632 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283070, 2, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 228, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (225.04, 63.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 316, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (331.50, 157.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 290, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (284.65, 121.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683226 sequences (100000118 bp) in 2.830 CPU sec, 3.244 real sec... [M::mem_process_seqs] Processed 683710 reads in 102.578 CPU sec, 3.569 real sec [M::process] read 682588 sequences (100000180 bp) in 2.252 CPU sec, 2.839 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 282892, 9, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (274, 466, 556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1120) [M::mem_pestat] mean and std.dev: (385.22, 180.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 320, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (334.30, 158.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1136) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 257, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (238.21, 83.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 42, #reads = 1078658 mem = 536876743 peak = 931909632 [M::mem_process_seqs] Processed 683226 reads in 98.750 CPU sec, 3.594 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282329, 10, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 399, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050) [M::mem_pestat] mean and std.dev: (321.90, 205.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1352) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 325, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (340.13, 161.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1166) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1469, 1978, 1978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (451, 2996) [M::mem_pestat] mean and std.dev: (1849.25, 333.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3505) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 240, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (231.22, 85.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 994) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680494 sequences (100000110 bp) in 2.965 CPU sec, 3.250 real sec... [M::mem_process_seqs] Processed 682588 reads in 100.578 CPU sec, 3.619 real sec sorting block 43, #reads = 1078652 mem = 536877912 peak = 931909632 [M::process] read 681718 sequences (100000218 bp) in 2.401 CPU sec, 2.762 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 281729, 5, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 304, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844) [M::mem_pestat] mean and std.dev: (331.47, 201.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1138) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (223, 344, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955) [M::mem_pestat] mean and std.dev: (354.90, 165.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1199) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (280, 365, 942) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2266) [M::mem_pestat] mean and std.dev: (552.57, 405.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2928) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680494 reads in 99.910 CPU sec, 3.560 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282480, 7, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 309, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (256.78, 101.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 331, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (344.62, 162.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1167) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 332, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1136) [M::mem_pestat] mean and std.dev: (347.50, 209.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1445) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683982 sequences (100000137 bp) in 2.521 CPU sec, 2.869 real sec... sorting block 44, #reads = 1078224 mem = 536880618 peak = 931909632 [M::mem_process_seqs] Processed 681718 reads in 97.225 CPU sec, 3.486 real sec [M::process] read 683670 sequences (100000161 bp) in 2.420 CPU sec, 2.904 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 283363, 11, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 316, 723) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1699) [M::mem_pestat] mean and std.dev: (338.56, 172.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2187) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 316, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (332.97, 159.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (684, 956, 2065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4827) [M::mem_pestat] mean and std.dev: (1183.18, 695.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6208) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1102) [M::mem_pestat] mean and std.dev: (280.23, 175.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1421) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683982 reads in 103.881 CPU sec, 3.638 real sec sorting block 45, #reads = 1077905 mem = 536882307 peak = 931909632 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283287, 13, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 251, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1129) [M::mem_pestat] mean and std.dev: (298.73, 154.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 320, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (337.40, 162.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1170) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (93, 1268, 1318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3768) [M::mem_pestat] mean and std.dev: (758.42, 575.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4993) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (272, 325, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (362.94, 164.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683210 sequences (100000297 bp) in 2.647 CPU sec, 3.085 real sec... [M::mem_process_seqs] Processed 683670 reads in 97.713 CPU sec, 3.485 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282302, 12, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 326, 715) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1755) [M::mem_pestat] mean and std.dev: (473.73, 395.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2275) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 683212 sequences (100000091 bp) in 2.455 CPU sec, 2.752 real sec... [M::mem_pestat] (25, 50, 75) percentile: (207, 321, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (337.39, 161.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1163) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (708, 1045, 3140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8004) [M::mem_pestat] mean and std.dev: (1680.00, 1460.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10436) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 289, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1125) [M::mem_pestat] mean and std.dev: (336.56, 146.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1431) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 46, #reads = 1080655 mem = 536881971 peak = 931909632 [M::mem_process_seqs] Processed 683210 reads in 99.203 CPU sec, 3.453 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 282171, 1, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 366, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1149) [M::mem_pestat] mean and std.dev: (351.94, 231.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 319, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (336.15, 160.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1155) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 397, 778) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1852) [M::mem_pestat] mean and std.dev: (406.93, 308.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2389) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682982 sequences (100000044 bp) in 2.637 CPU sec, 3.002 real sec... [M::mem_process_seqs] Processed 683212 reads in 95.513 CPU sec, 3.355 real sec sorting block 47, #reads = 1079593 mem = 536877906 peak = 931909632 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 282019, 13, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (234, 344, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (291.20, 97.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1162) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 320, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (336.66, 160.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (143, 488, 1193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3293) [M::mem_pestat] mean and std.dev: (718.92, 838.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4343) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 281, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1058) [M::mem_pestat] mean and std.dev: (321.21, 221.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1359) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682996 sequences (100000301 bp) in 2.382 CPU sec, 2.810 real sec... [M::mem_process_seqs] Processed 682982 reads in 99.192 CPU sec, 3.522 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 282273, 5, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 355, 1013) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2727) [M::mem_pestat] mean and std.dev: (549.68, 588.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 322, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (338.95, 162.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1168) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 340, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966) [M::mem_pestat] mean and std.dev: (334.78, 143.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1213) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682822 sequences (100000125 bp) in 2.964 CPU sec, 3.355 real sec... [M::mem_process_seqs] Processed 682996 reads in 95.901 CPU sec, 3.389 real sec sorting block 48, #reads = 1080058 mem = 536879740 peak = 931909632 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282357, 13, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 277, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (238.64, 117.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 323, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (340.23, 163.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1176) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (210, 438, 1101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2883) [M::mem_pestat] mean and std.dev: (563.08, 439.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3774) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 237, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (241.31, 96.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681144 sequences (100000078 bp) in 2.679 CPU sec, 2.987 real sec... [M::mem_process_seqs] Processed 682822 reads in 98.468 CPU sec, 3.422 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 281869, 21, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 327, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (341.40, 145.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (218, 334, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (347.73, 163.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (67, 393, 812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2302) [M::mem_pestat] mean and std.dev: (431.81, 390.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3047) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 317, 571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1261) [M::mem_pestat] mean and std.dev: (337.18, 209.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1606) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 49, #reads = 1080073 mem = 536884080 peak = 931909632 [M::process] read 680396 sequences (100000239 bp) in 2.566 CPU sec, 2.991 real sec... [M::mem_process_seqs] Processed 681144 reads in 96.827 CPU sec, 3.381 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 281301, 8, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 296, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (290.29, 101.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (221, 338, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (350.87, 163.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1177) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 343, 550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1230) [M::mem_pestat] mean and std.dev: (382.20, 213.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1570) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682510 sequences (100000132 bp) in 2.630 CPU sec, 2.897 real sec... [M::mem_process_seqs] Processed 680396 reads in 97.695 CPU sec, 3.392 real sec sorting block 50, #reads = 1080126 mem = 536876899 peak = 931909632 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 282519, 7, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 351, 733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1797) [M::mem_pestat] mean and std.dev: (446.21, 408.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2329) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 321, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (338.08, 161.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 333, 639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1553) [M::mem_pestat] mean and std.dev: (454.61, 357.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2010) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682494 sequences (100000283 bp) in 2.689 CPU sec, 3.108 real sec... [M::mem_process_seqs] Processed 682510 reads in 98.969 CPU sec, 3.520 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 281425, 11, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 465, 601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1375) [M::mem_pestat] mean and std.dev: (456.30, 296.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1762) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 323, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (339.87, 162.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1162) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1931, 2111, 3427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6419) [M::mem_pestat] mean and std.dev: (2373.45, 1108.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7915) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 300, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1112) [M::mem_pestat] mean and std.dev: (269.95, 117.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1408) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682512 sequences (100000224 bp) in 2.425 CPU sec, 2.817 real sec... sorting block 51, #reads = 1077566 mem = 536879932 peak = 931909632 [M::mem_process_seqs] Processed 682494 reads in 98.958 CPU sec, 3.589 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 281850, 9, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 325, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988) [M::mem_pestat] mean and std.dev: (309.39, 147.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1239) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 321, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (339.32, 163.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1168) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 271, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (287.71, 113.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682482 sequences (100000224 bp) in 2.793 CPU sec, 3.228 real sec... [M::mem_process_seqs] Processed 682512 reads in 101.451 CPU sec, 3.592 real sec sorting block 52, #reads = 1080330 mem = 536876565 peak = 931909632 [M::process] read 682706 sequences (100000183 bp) in 2.124 CPU sec, 2.433 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 281915, 7, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 220, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (216.56, 88.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 320, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (338.06, 162.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 286, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (271.75, 107.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682482 reads in 98.342 CPU sec, 3.574 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 281622, 8, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 319, 589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1285) [M::mem_pestat] mean and std.dev: (329.75, 159.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 319, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (338.01, 162.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 380, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (346.80, 125.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682622 sequences (100000097 bp) in 2.876 CPU sec, 3.108 real sec... [M::mem_process_seqs] Processed 682706 reads in 100.233 CPU sec, 3.478 real sec sorting block 53, #reads = 1079777 mem = 536880240 peak = 931909632 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 282188, 13, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 270, 823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2085) [M::mem_pestat] mean and std.dev: (515.76, 497.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 315, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (333.94, 159.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (194, 2006, 2317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6563) [M::mem_pestat] mean and std.dev: (1550.92, 1002.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8686) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 346, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (330.17, 157.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682826 sequences (100000153 bp) in 2.470 CPU sec, 2.838 real sec... [M::mem_process_seqs] Processed 682622 reads in 97.935 CPU sec, 3.447 real sec sorting block 54, #reads = 1080280 mem = 536880676 peak = 931909632 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282177, 11, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 358, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (292.00, 109.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 316, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (335.39, 161.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1362, 1362, 3440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7596) [M::mem_pestat] mean and std.dev: (2274.36, 1637.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9674) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (133, 211, 791) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2107) [M::mem_pestat] mean and std.dev: (455.07, 415.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2765) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682990 sequences (100000103 bp) in 2.782 CPU sec, 3.154 real sec... [M::mem_process_seqs] Processed 682826 reads in 98.185 CPU sec, 3.345 real sec [M::process] read 682870 sequences (100000268 bp) in 2.485 CPU sec, 2.768 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 281775, 3, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 363, 584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1394) [M::mem_pestat] mean and std.dev: (347.24, 212.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 315, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (334.89, 161.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 311, 642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1474) [M::mem_pestat] mean and std.dev: (430.37, 310.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1890) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682990 reads in 100.083 CPU sec, 3.508 real sec sorting block 55, #reads = 1079241 mem = 536877685 peak = 931909632 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 282438, 10, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 281, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1150) [M::mem_pestat] mean and std.dev: (347.15, 208.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 315, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (334.90, 162.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (662, 757, 5327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14657) [M::mem_pestat] mean and std.dev: (2556.50, 2262.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19322) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 267, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1163) [M::mem_pestat] mean and std.dev: (302.86, 194.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1497) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682846 sequences (100000274 bp) in 2.751 CPU sec, 3.009 real sec... [M::mem_process_seqs] Processed 682870 reads in 99.874 CPU sec, 3.597 real sec sorting block 56, #reads = 1080287 mem = 536878740 peak = 939880448 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 282047, 4, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 393, 1905) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5263) [M::mem_pestat] mean and std.dev: (1093.08, 1142.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6942) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 317, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (335.76, 162.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 258, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1167) [M::mem_pestat] mean and std.dev: (330.05, 274.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1500) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682568 sequences (100000029 bp) in 2.754 CPU sec, 3.004 real sec... [M::mem_process_seqs] Processed 682846 reads in 99.414 CPU sec, 3.432 real sec [M::process] read 682776 sequences (100000229 bp) in 2.488 CPU sec, 2.847 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 281900, 6, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 298, 623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1499) [M::mem_pestat] mean and std.dev: (333.36, 220.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1937) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 317, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (336.24, 161.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (141, 284, 597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1509) [M::mem_pestat] mean and std.dev: (354.00, 234.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1965) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682568 reads in 97.479 CPU sec, 3.349 real sec sorting block 57, #reads = 1079451 mem = 536877968 peak = 939880448 [M::process] read 682676 sequences (100000296 bp) in 2.241 CPU sec, 2.456 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 282507, 8, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 199, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (225.68, 99.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 318, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921) [M::mem_pestat] mean and std.dev: (337.03, 163.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1159) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 315, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (333.15, 159.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 282111, 7, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 246, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (279.09, 185.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1021) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 318, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921) [M::mem_pestat] mean and std.dev: (337.14, 163.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1159) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 294, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1163) [M::mem_pestat] mean and std.dev: (350.18, 200.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1484) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682410 sequences (100000209 bp) in 2.447 CPU sec, 2.678 real sec... sorting block 58, #reads = 1079186 mem = 536875776 peak = 992608256 [M::mem_process_seqs] Processed 682776 reads in 172.452 CPU sec, 5.667 real sec [M::mem_process_seqs] Processed 682676 reads in 116.634 CPU sec, 3.800 real sec [M::process] read 683008 sequences (100000064 bp) in 2.723 CPU sec, 2.969 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282759, 5, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 378, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009) [M::mem_pestat] mean and std.dev: (344.29, 204.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1278) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 319, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (338.70, 164.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1171) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 209, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (223.00, 92.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 59, #reads = 1079781 mem = 536877890 peak = 992608256 [M::process] read 683588 sequences (100000275 bp) in 2.238 CPU sec, 2.550 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 282740, 13, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 279, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1086) [M::mem_pestat] mean and std.dev: (288.39, 203.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 317, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (338.72, 166.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1178) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (869, 1435, 2794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6644) [M::mem_pestat] mean and std.dev: (1614.38, 788.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8569) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 230, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (95, 360) [M::mem_pestat] mean and std.dev: (218.32, 38.57) [M::mem_pestat] low and high boundaries for proper pairs: (42, 413) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682410 reads in 178.661 CPU sec, 5.879 real sec [M::mem_process_seqs] Processed 683008 reads in 119.341 CPU sec, 3.940 real sec [M::process] read 682112 sequences (100000269 bp) in 2.538 CPU sec, 2.699 real sec... sorting block 60, #reads = 1079018 mem = 536880196 peak = 992608256 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 281749, 12, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 251, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016) [M::mem_pestat] mean and std.dev: (291.83, 173.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1284) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 317, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (331.63, 156.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1100, 2575, 2575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5525) [M::mem_pestat] mean and std.dev: (1944.08, 667.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7000) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (315, 414, 950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2220) [M::mem_pestat] mean and std.dev: (499.66, 447.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2855) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 281762, 9, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 204, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1170) [M::mem_pestat] mean and std.dev: (227.24, 140.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 322, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (335.92, 156.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (397, 530, 1367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3307) [M::mem_pestat] mean and std.dev: (722.94, 466.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4277) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681784 sequences (100000295 bp) in 2.517 CPU sec, 2.978 real sec... [M::mem_process_seqs] Processed 683588 reads in 172.999 CPU sec, 5.695 real sec [M::mem_process_seqs] Processed 682112 reads in 114.452 CPU sec, 3.794 real sec sorting block 61, #reads = 1078589 mem = 536880861 peak = 992608256 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 281678, 18, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 239, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (261.30, 140.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 325, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (339.30, 158.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (56, 97, 812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2324) [M::mem_pestat] mean and std.dev: (330.47, 362.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3080) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 259, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (298.86, 108.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681406 sequences (100000028 bp) in 2.734 CPU sec, 2.975 real sec... [M::mem_process_seqs] Processed 681784 reads in 96.103 CPU sec, 3.280 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 281200, 1, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 215, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (217.30, 80.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 682524 sequences (100000199 bp) in 2.640 CPU sec, 2.870 real sec... [M::mem_pestat] (25, 50, 75) percentile: (214, 326, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (340.54, 159.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1142) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 384, 640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1602) [M::mem_pestat] mean and std.dev: (337.24, 195.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2083) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681406 reads in 95.333 CPU sec, 3.234 real sec sorting block 62, #reads = 1078532 mem = 536878981 peak = 992608256 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 281385, 5, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 249, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (272.52, 103.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 319, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (334.29, 156.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 251, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (267.04, 114.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682784 sequences (100000064 bp) in 2.558 CPU sec, 2.949 real sec... [M::mem_process_seqs] Processed 682524 reads in 96.407 CPU sec, 3.263 real sec sorting block 63, #reads = 1078384 mem = 536878567 peak = 992608256 [M::process] read 682642 sequences (100000221 bp) in 2.445 CPU sec, 2.618 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 281665, 12, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 202, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (222.65, 101.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 319, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (333.61, 157.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1089, 1426, 1426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (415, 2100) [M::mem_pestat] mean and std.dev: (1224.50, 217.11) [M::mem_pestat] low and high boundaries for proper pairs: (78, 2437) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (273, 328, 551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1107) [M::mem_pestat] mean and std.dev: (317.88, 111.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1385) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682784 reads in 91.094 CPU sec, 3.131 real sec [M::process] read 679994 sequences (100000245 bp) in 2.466 CPU sec, 2.743 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 281597, 12, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 234, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (216.05, 58.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 323, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (337.90, 159.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1150) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1354, 1354, 2574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5014) [M::mem_pestat] mean and std.dev: (1730.17, 1011.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6234) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 223, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (327.80, 211.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682642 reads in 97.043 CPU sec, 3.283 real sec sorting block 64, #reads = 1079726 mem = 536880065 peak = 992608256 [M::process] read 681296 sequences (100000016 bp) in 2.214 CPU sec, 2.577 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 280483, 2, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 224, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (282.43, 121.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (223, 340, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937) [M::mem_pestat] mean and std.dev: (351.71, 162.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1175) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (289, 295, 1001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2425) [M::mem_pestat] mean and std.dev: (490.13, 419.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3137) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 679994 reads in 92.130 CPU sec, 3.129 real sec sorting block 65, #reads = 1079515 mem = 536877718 peak = 992608256 [M::process] read 683650 sequences (100000262 bp) in 2.617 CPU sec, 2.845 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 282031, 20, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 376, 965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2521) [M::mem_pestat] mean and std.dev: (551.59, 453.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3299) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (218, 332, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (346.08, 162.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1162) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (2523, 5676, 5676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11982) [M::mem_pestat] mean and std.dev: (4329.40, 1885.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15135) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 287, 590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1396) [M::mem_pestat] mean and std.dev: (414.58, 299.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1799) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 283214, 11, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 187, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002) [M::mem_pestat] mean and std.dev: (280.82, 223.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1297) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 318, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (334.12, 160.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (458, 1158, 1158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2558) [M::mem_pestat] mean and std.dev: (963.18, 593.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3337) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 389, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1242) [M::mem_pestat] mean and std.dev: (304.24, 163.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1603) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683622 sequences (100000091 bp) in 2.509 CPU sec, 2.821 real sec... [M::mem_process_seqs] Processed 681296 reads in 171.670 CPU sec, 5.625 real sec [M::mem_process_seqs] Processed 683650 reads in 114.892 CPU sec, 3.744 real sec sorting block 66, #reads = 1078686 mem = 536880094 peak = 992608256 [M::process] read 683814 sequences (100000049 bp) in 2.508 CPU sec, 2.736 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282458, 16, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 329, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (286.56, 94.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 321, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (336.19, 161.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1158) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (272, 565, 1266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3254) [M::mem_pestat] mean and std.dev: (614.07, 434.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4248) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 253, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (285.80, 166.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1192) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 282744, 4, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 329, 578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1338) [M::mem_pestat] mean and std.dev: (366.82, 246.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 317, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (333.80, 160.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 359, 766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1856) [M::mem_pestat] mean and std.dev: (459.09, 393.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2401) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683770 sequences (100000076 bp) in 2.714 CPU sec, 2.991 real sec... [M::mem_process_seqs] Processed 683622 reads in 175.006 CPU sec, 5.735 real sec sorting block 67, #reads = 1080786 mem = 536875835 peak = 992608256 [M::mem_process_seqs] Processed 683814 reads in 116.567 CPU sec, 3.807 real sec [M::process] read 683632 sequences (100000017 bp) in 2.329 CPU sec, 2.630 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 282439, 5, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (222, 359, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (300.23, 107.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1170) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 315, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (331.73, 158.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 275, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (285.67, 128.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1006) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683770 reads in 98.839 CPU sec, 3.356 real sec sorting block 68, #reads = 1080021 mem = 536877281 peak = 992608256 [M::process] read 683488 sequences (100000277 bp) in 2.417 CPU sec, 2.703 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 282645, 17, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 227, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (250.97, 132.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 317, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (335.04, 161.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (671, 709, 993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (27, 1637) [M::mem_pestat] mean and std.dev: (752.38, 313.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2007) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 276, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042) [M::mem_pestat] mean and std.dev: (345.61, 191.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1318) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683632 reads in 96.607 CPU sec, 3.313 real sec [M::process] read 683382 sequences (100000065 bp) in 2.533 CPU sec, 2.789 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 282107, 13, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 349, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (311.73, 131.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 317, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (334.60, 160.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (156, 194, 1338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3702) [M::mem_pestat] mean and std.dev: (482.85, 495.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4884) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 284, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1227) [M::mem_pestat] mean and std.dev: (276.23, 152.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1581) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 69, #reads = 1079866 mem = 536879211 peak = 992608256 [M::mem_process_seqs] Processed 683488 reads in 99.546 CPU sec, 3.341 real sec [M::process] read 681056 sequences (100000043 bp) in 1.973 CPU sec, 2.226 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 282057, 15, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 216, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (264.75, 127.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 318, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (336.06, 161.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1158) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1293, 2145, 2145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3849) [M::mem_pestat] mean and std.dev: (1602.47, 833.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4937) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 304, 660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1534) [M::mem_pestat] mean and std.dev: (387.12, 289.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1971) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 70, #reads = 1079846 mem = 536876458 peak = 1029025792 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 281422, 15, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 267, 826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2132) [M::mem_pestat] mean and std.dev: (518.95, 522.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2785) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (217, 333, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937) [M::mem_pestat] mean and std.dev: (347.19, 163.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1177) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (552, 552, 1278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2730) [M::mem_pestat] mean and std.dev: (782.20, 447.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3456) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 282, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (260.88, 107.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681148 sequences (100000037 bp) in 2.593 CPU sec, 2.812 real sec... [M::mem_process_seqs] Processed 683382 reads in 176.678 CPU sec, 5.819 real sec [M::mem_process_seqs] Processed 681056 reads in 118.649 CPU sec, 3.856 real sec [M::process] read 682896 sequences (100000116 bp) in 2.230 CPU sec, 2.608 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 280757, 11, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 215, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (218.38, 77.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 331, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (345.92, 162.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1168) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (340, 340, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (340, 340) [M::mem_pestat] mean and std.dev: (340.00, 0.00) [M::mem_pestat] low and high boundaries for proper pairs: (340, 340) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 284, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046) [M::mem_pestat] mean and std.dev: (317.65, 134.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1326) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681148 reads in 95.276 CPU sec, 3.257 real sec sorting block 71, #reads = 1080102 mem = 536878297 peak = 1029025792 [M::process] read 683028 sequences (100000204 bp) in 2.687 CPU sec, 2.954 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282150, 7, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 242, 573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1325) [M::mem_pestat] mean and std.dev: (244.04, 104.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 320, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (338.04, 161.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 270, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1309) [M::mem_pestat] mean and std.dev: (317.85, 217.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1684) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 72, #reads = 1079670 mem = 536879474 peak = 1029025792 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282313, 10, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 217, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051) [M::mem_pestat] mean and std.dev: (309.37, 216.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1344) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 319, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (336.88, 161.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (289, 532, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018) [M::mem_pestat] mean and std.dev: (414.50, 163.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1261) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 255, 577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1399) [M::mem_pestat] mean and std.dev: (343.29, 268.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1810) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682078 sequences (100000048 bp) in 2.381 CPU sec, 2.655 real sec... [M::mem_process_seqs] Processed 682896 reads in 178.155 CPU sec, 5.848 real sec [M::mem_process_seqs] Processed 683028 reads in 117.638 CPU sec, 3.875 real sec [M::process] read 681474 sequences (100000145 bp) in 2.245 CPU sec, 2.657 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282086, 8, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 307, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (304.94, 151.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 326, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932) [M::mem_pestat] mean and std.dev: (342.22, 162.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (282, 310, 751) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1689) [M::mem_pestat] mean and std.dev: (391.03, 258.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2158) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 73, #reads = 1079564 mem = 536879204 peak = 1029025792 [M::process] read 682774 sequences (100000201 bp) in 2.474 CPU sec, 2.771 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 281571, 6, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 316, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (274.70, 104.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 330, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (346.10, 164.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1179) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (290, 539, 939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2237) [M::mem_pestat] mean and std.dev: (677.19, 591.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3043) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682078 reads in 178.391 CPU sec, 5.974 real sec [M::mem_process_seqs] Processed 681474 reads in 116.488 CPU sec, 3.931 real sec [M::process] read 682754 sequences (100000131 bp) in 2.763 CPU sec, 2.966 real sec... sorting block 74, #reads = 1078851 mem = 536877533 peak = 1029025792 [M::process] read 682818 sequences (100000279 bp) in 2.465 CPU sec, 2.862 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 281865, 11, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 225, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (195.78, 86.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 318, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921) [M::mem_pestat] mean and std.dev: (336.87, 161.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1159) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (753, 2021, 2021) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4557) [M::mem_pestat] mean and std.dev: (1403.27, 699.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5825) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 248, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (266.26, 137.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682754 reads in 99.217 CPU sec, 3.345 real sec sorting block 75, #reads = 1079641 mem = 536878528 peak = 1029025792 [M::process] read 683082 sequences (100000222 bp) in 2.643 CPU sec, 2.878 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282150, 8, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 255, 579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1339) [M::mem_pestat] mean and std.dev: (289.00, 186.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1719) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 315, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (332.91, 159.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1138) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 501, 743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1745) [M::mem_pestat] mean and std.dev: (442.65, 257.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2246) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 282214, 7, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (250, 344, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027) [M::mem_pestat] mean and std.dev: (433.10, 253.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 321, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (337.90, 161.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 261, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1176) [M::mem_pestat] mean and std.dev: (298.97, 167.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1515) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683206 sequences (100000194 bp) in 2.047 CPU sec, 2.187 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 281611, 6, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 244, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (293.70, 154.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 314, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (333.11, 160.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 289, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1178) [M::mem_pestat] mean and std.dev: (318.58, 171.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1505) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682818 reads in 181.643 CPU sec, 5.862 real sec [M::mem_process_seqs] Processed 682774 reads in 382.073 CPU sec, 12.536 real sec [M::mem_process_seqs] Processed 683082 reads in 137.726 CPU sec, 4.502 real sec sorting block 76, #reads = 1079484 mem = 536876941 peak = 1029025792 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 282205, 4, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 236, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (242.90, 126.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1170) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 314, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (332.16, 158.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 273, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (308.02, 140.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683206 sequences (100000052 bp) in 2.870 CPU sec, 3.142 real sec... [M::mem_process_seqs] Processed 683206 reads in 103.987 CPU sec, 3.605 real sec sorting block 77, #reads = 1078035 mem = 536878201 peak = 1029025792 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 282401, 4, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 255, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (261.09, 147.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 313, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (332.45, 159.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (131, 263, 720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1898) [M::mem_pestat] mean and std.dev: (407.25, 365.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2487) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682848 sequences (100000133 bp) in 2.508 CPU sec, 2.829 real sec... [M::mem_process_seqs] Processed 683206 reads in 98.489 CPU sec, 3.353 real sec [M::process] read 682976 sequences (100000297 bp) in 2.269 CPU sec, 2.649 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282343, 8, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 250, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (240.56, 101.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 316, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (335.00, 161.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1150) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (252, 345, 766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1794) [M::mem_pestat] mean and std.dev: (472.51, 355.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2308) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682848 reads in 100.038 CPU sec, 3.434 real sec sorting block 78, #reads = 1078245 mem = 536880923 peak = 1029025792 [M::process] read 683114 sequences (100000252 bp) in 2.340 CPU sec, 2.514 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282354, 9, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 304, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1079) [M::mem_pestat] mean and std.dev: (335.59, 233.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1381) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 317, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (335.82, 161.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1158) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (134, 165, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (230.52, 162.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1022) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 79, #reads = 1079102 mem = 536880669 peak = 1029025792 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282654, 5, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 251, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 479) [M::mem_pestat] mean and std.dev: (229.18, 65.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 317, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (335.82, 162.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 262, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (264.74, 109.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683490 sequences (100000182 bp) in 2.622 CPU sec, 3.048 real sec... [M::mem_process_seqs] Processed 682976 reads in 177.194 CPU sec, 5.850 real sec [M::mem_process_seqs] Processed 683114 reads in 118.640 CPU sec, 3.915 real sec [M::process] read 687104 sequences (100000228 bp) in 2.506 CPU sec, 2.767 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283136, 9, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 216, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (261.65, 166.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1092) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 313, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (334.05, 162.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 277, 621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1407) [M::mem_pestat] mean and std.dev: (428.10, 306.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1800) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 80, #reads = 1079836 mem = 536878102 peak = 1029025792 [M::process] read 685368 sequences (100000187 bp) in 2.182 CPU sec, 2.471 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 284223, 6, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 194, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (220.96, 98.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 296, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (321.88, 162.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 238, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (281.88, 114.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683490 reads in 183.180 CPU sec, 6.070 real sec [M::mem_process_seqs] Processed 687104 reads in 124.933 CPU sec, 4.142 real sec [M::process] read 684836 sequences (100000178 bp) in 2.549 CPU sec, 2.844 real sec... sorting block 81, #reads = 1078771 mem = 536879331 peak = 1029025792 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 283455, 2, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 217, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1293) [M::mem_pestat] mean and std.dev: (265.55, 196.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 314, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964) [M::mem_pestat] mean and std.dev: (337.42, 169.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1219) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 344, 1102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2862) [M::mem_pestat] mean and std.dev: (516.51, 451.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3742) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685670 sequences (100000267 bp) in 2.877 CPU sec, 3.204 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283561, 3, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 272, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (258.63, 89.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 317, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969) [M::mem_pestat] mean and std.dev: (339.54, 169.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1225) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 202, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (195.00, 65.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685368 reads in 189.668 CPU sec, 6.216 real sec [M::mem_process_seqs] Processed 684836 reads in 124.093 CPU sec, 4.039 real sec sorting block 82, #reads = 1076733 mem = 536883181 peak = 1029025792 [M::process] read 686694 sequences (100000159 bp) in 2.152 CPU sec, 2.338 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 284303, 8, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 275, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804) [M::mem_pestat] mean and std.dev: (245.75, 118.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1004) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 311, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951) [M::mem_pestat] mean and std.dev: (334.22, 167.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1202) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (127, 250, 820) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2206) [M::mem_pestat] mean and std.dev: (410.94, 396.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2899) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686454 sequences (100000261 bp) in 2.590 CPU sec, 3.004 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 283723, 2, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 297, 640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1590) [M::mem_pestat] mean and std.dev: (395.07, 381.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2065) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 302, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (325.64, 163.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 249, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (237.48, 103.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685670 reads in 188.975 CPU sec, 6.152 real sec sorting block 83, #reads = 1077676 mem = 536878656 peak = 1029025792 [M::process] read 686820 sequences (100000085 bp) in 2.370 CPU sec, 2.559 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 284232, 5, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 275, 781) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2039) [M::mem_pestat] mean and std.dev: (500.26, 486.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 304, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (328.13, 164.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (257, 378, 654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1448) [M::mem_pestat] mean and std.dev: (366.79, 170.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1845) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686454 reads in 103.364 CPU sec, 3.396 real sec [M::process] read 684202 sequences (100000104 bp) in 2.867 CPU sec, 3.018 real sec... sorting block 84, #reads = 1077843 mem = 536882612 peak = 1029025792 [M::mem_process_seqs] Processed 686694 reads in 307.669 CPU sec, 9.970 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 284650, 8, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 213, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (260.92, 172.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1183) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 300, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (324.08, 162.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (293, 345, 506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932) [M::mem_pestat] mean and std.dev: (370.94, 164.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683336 sequences (100000236 bp) in 2.379 CPU sec, 2.709 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 283301, 4, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 342, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (317.54, 160.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 320, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953) [M::mem_pestat] mean and std.dev: (339.12, 166.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1203) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (142, 254, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1132) [M::mem_pestat] mean and std.dev: (268.68, 175.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1462) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686820 reads in 208.650 CPU sec, 6.738 real sec [M::mem_process_seqs] Processed 684202 reads in 122.507 CPU sec, 3.976 real sec sorting block 85, #reads = 1079286 mem = 536878165 peak = 1029025792 [M::process] read 686408 sequences (100000134 bp) in 2.496 CPU sec, 2.736 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283245, 11, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 243, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (257.40, 154.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1138) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 325, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954) [M::mem_pestat] mean and std.dev: (342.02, 165.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1203) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (427, 427, 1422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3412) [M::mem_pestat] mean and std.dev: (799.27, 492.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4407) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (250, 375, 680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1540) [M::mem_pestat] mean and std.dev: (506.87, 369.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1985) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 86, #reads = 1078355 mem = 536881145 peak = 1029025792 [M::process] read 686670 sequences (100000295 bp) in 2.708 CPU sec, 2.980 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 283992, 6, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 224, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (253.04, 109.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1138) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 303, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (325.46, 161.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1158) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (323, 493, 1815) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4799) [M::mem_pestat] mean and std.dev: (989.48, 906.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6291) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683336 reads in 188.357 CPU sec, 6.226 real sec [M::mem_process_seqs] Processed 686408 reads in 123.977 CPU sec, 4.184 real sec [M::process] read 686230 sequences (100000148 bp) in 2.359 CPU sec, 2.729 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 283989, 15, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 249, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989) [M::mem_pestat] mean and std.dev: (301.35, 170.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1262) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 300, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (323.10, 160.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (467, 1426, 1915) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4811) [M::mem_pestat] mean and std.dev: (1207.07, 738.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6259) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 249, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (251.29, 104.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 87, #reads = 1076303 mem = 536879680 peak = 1029025792 [M::process] read 685506 sequences (100000243 bp) in 2.498 CPU sec, 2.909 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 283970, 6, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (266, 314, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (26, 626) [M::mem_pestat] mean and std.dev: (271.39, 93.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 305, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921) [M::mem_pestat] mean and std.dev: (327.00, 162.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1163) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 470, 607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1381) [M::mem_pestat] mean and std.dev: (357.95, 142.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1768) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686670 reads in 196.166 CPU sec, 6.540 real sec [M::mem_process_seqs] Processed 686230 reads in 125.439 CPU sec, 4.230 real sec [M::process] read 683912 sequences (100000115 bp) in 2.388 CPU sec, 2.639 real sec... sorting block 88, #reads = 1077792 mem = 536882282 peak = 1029025792 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 283610, 12, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 300, 542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1210) [M::mem_pestat] mean and std.dev: (325.78, 167.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 311, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936) [M::mem_pestat] mean and std.dev: (331.76, 163.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1182) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1821, 2512, 2512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (439, 3894) [M::mem_pestat] mean and std.dev: (2095.73, 617.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4585) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 336, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (328.38, 135.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682004 sequences (100000116 bp) in 2.682 CPU sec, 3.125 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 282922, 13, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 217, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (216.36, 97.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 319, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948) [M::mem_pestat] mean and std.dev: (337.92, 164.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1196) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (520, 520, 4887) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13621) [M::mem_pestat] mean and std.dev: (1585.46, 1816.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17988) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 310, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (297.92, 132.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685506 reads in 193.502 CPU sec, 6.334 real sec [M::mem_process_seqs] Processed 683912 reads in 127.867 CPU sec, 4.177 real sec [M::process] read 684328 sequences (100000044 bp) in 2.415 CPU sec, 2.537 real sec... sorting block 89, #reads = 1077139 mem = 536880340 peak = 1029025792 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 282002, 10, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 265, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1219) [M::mem_pestat] mean and std.dev: (353.88, 214.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 332, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971) [M::mem_pestat] mean and std.dev: (349.03, 167.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1224) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (127, 228, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (179.44, 96.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 285, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (275.31, 94.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684316 sequences (100000077 bp) in 2.755 CPU sec, 3.106 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 283423, 8, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 269, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (262.24, 154.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 316, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (336.28, 164.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1194) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 323, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1064) [M::mem_pestat] mean and std.dev: (346.32, 171.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1342) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682004 reads in 191.382 CPU sec, 6.214 real sec sorting block 90, #reads = 1077477 mem = 536877997 peak = 1029025792 [M::process] read 682326 sequences (100000178 bp) in 1.940 CPU sec, 2.048 real sec... [M::mem_process_seqs] Processed 684328 reads in 127.905 CPU sec, 4.149 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 283011, 8, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 338, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1079) [M::mem_pestat] mean and std.dev: (361.53, 171.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1367) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 314, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931) [M::mem_pestat] mean and std.dev: (334.10, 162.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 261, 1025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2693) [M::mem_pestat] mean and std.dev: (485.30, 393.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3527) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 91, #reads = 1076763 mem = 536876351 peak = 1071730688 [M::process] read 683560 sequences (100000182 bp) in 3.108 CPU sec, 3.441 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282118, 17, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 251, 541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1239) [M::mem_pestat] mean and std.dev: (281.14, 146.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 326, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943) [M::mem_pestat] mean and std.dev: (343.40, 164.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1187) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (100, 252, 790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2170) [M::mem_pestat] mean and std.dev: (383.62, 400.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2860) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 377, 644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1584) [M::mem_pestat] mean and std.dev: (385.80, 303.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2054) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684316 reads in 192.660 CPU sec, 6.276 real sec [M::mem_process_seqs] Processed 682326 reads in 128.991 CPU sec, 4.129 real sec [M::process] read 682886 sequences (100000283 bp) in 2.522 CPU sec, 2.750 real sec... sorting block 92, #reads = 1075577 mem = 536878188 peak = 1071730688 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282799, 12, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (249, 272, 631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1395) [M::mem_pestat] mean and std.dev: (404.85, 294.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 314, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (332.93, 159.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1047, 1681, 2424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5178) [M::mem_pestat] mean and std.dev: (1509.55, 826.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6555) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 316, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1086) [M::mem_pestat] mean and std.dev: (347.84, 194.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1375) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682910 sequences (100000291 bp) in 2.560 CPU sec, 3.024 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282601, 8, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 298, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (309.86, 190.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1092) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 319, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (336.92, 161.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1170) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 267, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021) [M::mem_pestat] mean and std.dev: (304.93, 137.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1298) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683560 reads in 192.441 CPU sec, 6.272 real sec [M::mem_process_seqs] Processed 682886 reads in 130.423 CPU sec, 4.192 real sec [M::process] read 681252 sequences (100000127 bp) in 1.953 CPU sec, 2.059 real sec... sorting block 93, #reads = 1075427 mem = 536880327 peak = 1071730688 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282556, 7, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 280, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (272.38, 133.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 320, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (338.76, 163.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1175) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 205, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (287.92, 147.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683598 sequences (100000237 bp) in 2.972 CPU sec, 3.507 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281967, 9, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 205, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (268.67, 166.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 334, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965) [M::mem_pestat] mean and std.dev: (349.92, 166.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1215) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 245, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1102) [M::mem_pestat] mean and std.dev: (267.88, 126.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1407) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682910 reads in 193.177 CPU sec, 6.281 real sec [M::mem_process_seqs] Processed 681252 reads in 133.002 CPU sec, 4.263 real sec sorting block 94, #reads = 1075531 mem = 536885282 peak = 1071730688 [M::process] read 683766 sequences (100000289 bp) in 2.566 CPU sec, 2.698 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282868, 15, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 301, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1120) [M::mem_pestat] mean and std.dev: (357.38, 233.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 315, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932) [M::mem_pestat] mean and std.dev: (335.06, 162.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1175) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (314, 831, 1215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3017) [M::mem_pestat] mean and std.dev: (860.73, 631.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3918) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 283, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (274.92, 115.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 95, #reads = 1074172 mem = 536884044 peak = 1071730688 [M::process] read 682458 sequences (100000091 bp) in 2.721 CPU sec, 3.027 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 283379, 10, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 251, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004) [M::mem_pestat] mean and std.dev: (239.10, 117.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1292) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 312, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (332.24, 160.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1159) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (93, 160, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1080) [M::mem_pestat] mean and std.dev: (331.50, 371.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1819) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (246, 259, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (293.59, 131.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683598 reads in 194.069 CPU sec, 6.366 real sec [M::process] read 682440 sequences (100000173 bp) in 1.670 CPU sec, 1.732 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 281996, 9, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 259, 512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1132) [M::mem_pestat] mean and std.dev: (314.97, 172.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 321, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937) [M::mem_pestat] mean and std.dev: (339.28, 162.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1180) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 252, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017) [M::mem_pestat] mean and std.dev: (339.31, 190.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1293) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683220 sequences (100000084 bp) in 2.253 CPU sec, 2.340 real sec... [M::mem_process_seqs] Processed 682458 reads in 112.462 CPU sec, 3.787 real sec sorting block 96, #reads = 1074371 mem = 536881533 peak = 1071730688 [M::mem_process_seqs] Processed 683766 reads in 297.332 CPU sec, 9.863 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 282774, 12, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 256, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949) [M::mem_pestat] mean and std.dev: (286.78, 167.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1219) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 322, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943) [M::mem_pestat] mean and std.dev: (341.13, 165.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1188) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (252, 703, 1757) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4767) [M::mem_pestat] mean and std.dev: (890.17, 761.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6272) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 304, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (285.49, 96.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683120 sequences (100000136 bp) in 2.611 CPU sec, 2.941 real sec... sorting block 97, #reads = 1075777 mem = 536878402 peak = 1071730688 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282623, 5, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 168, 536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1302) [M::mem_pestat] mean and std.dev: (210.30, 127.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 317, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (336.40, 163.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 282, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (327.74, 198.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682440 reads in 207.660 CPU sec, 6.792 real sec [M::mem_process_seqs] Processed 683220 reads in 126.306 CPU sec, 4.128 real sec [M::process] read 681960 sequences (100000103 bp) in 2.512 CPU sec, 2.801 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 283214, 5, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 284, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1309) [M::mem_pestat] mean and std.dev: (323.49, 207.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 318, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940) [M::mem_pestat] mean and std.dev: (338.68, 165.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1185) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 324, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052) [M::mem_pestat] mean and std.dev: (308.00, 133.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1338) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 98, #reads = 1075957 mem = 536876945 peak = 1071730688 [M::process] read 687662 sequences (100000287 bp) in 2.503 CPU sec, 2.869 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 282602, 3, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 286, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (257.23, 105.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 325, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957) [M::mem_pestat] mean and std.dev: (345.25, 167.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1206) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 216, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (220.88, 90.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683120 reads in 186.522 CPU sec, 6.061 real sec [M::mem_process_seqs] Processed 681960 reads in 124.431 CPU sec, 4.035 real sec [M::process] read 690120 sequences (100000131 bp) in 2.090 CPU sec, 2.198 real sec... sorting block 99, #reads = 1075060 mem = 536878743 peak = 1071730688 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 284586, 4, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 356, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (300.50, 143.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1155) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 298, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (321.28, 161.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1150) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 473, 830) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2064) [M::mem_pestat] mean and std.dev: (489.83, 403.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2681) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687508 sequences (100000265 bp) in 2.844 CPU sec, 3.255 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 285728, 7, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 206, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (220.46, 88.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 285, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (308.53, 154.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 324, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (313.77, 142.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687662 reads in 211.444 CPU sec, 6.842 real sec [M::mem_process_seqs] Processed 690120 reads in 142.950 CPU sec, 4.595 real sec [M::process] read 688226 sequences (100000292 bp) in 2.670 CPU sec, 2.947 real sec... sorting block 100, #reads = 1074723 mem = 536878524 peak = 1071730688 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 284769, 3, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 389, 628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1506) [M::mem_pestat] mean and std.dev: (419.19, 323.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1945) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 300, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (319.82, 156.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (288, 323, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (351.13, 118.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686996 sequences (100000205 bp) in 3.016 CPU sec, 3.355 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 285067, 6, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 186, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (13, 388) [M::mem_pestat] mean and std.dev: (182.29, 62.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 294, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (315.00, 154.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (141, 280, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (229.06, 95.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1089) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687508 reads in 211.502 CPU sec, 6.856 real sec [M::process] read 687050 sequences (100000089 bp) in 2.122 CPU sec, 2.172 real sec... sorting block 101, #reads = 1071424 mem = 536879702 peak = 1071730688 [M::mem_process_seqs] Processed 688226 reads in 137.555 CPU sec, 4.422 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 284146, 4, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 193, 540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1310) [M::mem_pestat] mean and std.dev: (312.43, 221.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 301, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (320.41, 157.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 361, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (308.90, 111.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 102, #reads = 1072342 mem = 536878728 peak = 1071730688 [M::process] read 687186 sequences (100000134 bp) in 3.023 CPU sec, 3.439 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 284074, 10, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 217, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (215.00, 84.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 300, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (318.95, 155.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (130, 626, 626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1618) [M::mem_pestat] mean and std.dev: (449.20, 352.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2114) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 282, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (304.31, 144.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1092) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686996 reads in 204.867 CPU sec, 6.680 real sec [M::mem_process_seqs] Processed 687050 reads in 134.191 CPU sec, 4.373 real sec [M::process] read 684806 sequences (100000236 bp) in 2.283 CPU sec, 2.508 real sec... sorting block 103, #reads = 1073788 mem = 536878627 peak = 1071730688 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 284724, 12, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 282, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (245.49, 62.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 299, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (318.34, 156.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (555, 1200, 4209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11517) [M::mem_pestat] mean and std.dev: (1989.42, 1682.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15171) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 280, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962) [M::mem_pestat] mean and std.dev: (329.54, 210.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1232) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686612 sequences (100000112 bp) in 2.225 CPU sec, 2.483 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 284143, 5, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 302, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1152) [M::mem_pestat] mean and std.dev: (356.65, 189.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 315, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (332.26, 161.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1165) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 242, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (241.55, 124.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686890 sequences (100000242 bp) in 2.070 CPU sec, 2.138 real sec... [M::mem_process_seqs] Processed 687186 reads in 199.852 CPU sec, 6.462 real sec [M::mem_process_seqs] Processed 684806 reads in 126.424 CPU sec, 4.048 real sec sorting block 104, #reads = 1074074 mem = 536877550 peak = 1071730688 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 284584, 9, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 228, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (249.24, 148.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 300, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (320.62, 157.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 306, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931) [M::mem_pestat] mean and std.dev: (300.04, 162.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1175) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686750 sequences (100000111 bp) in 2.998 CPU sec, 3.639 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 284364, 3, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 343, 618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1478) [M::mem_pestat] mean and std.dev: (444.49, 375.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1948) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 300, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (320.33, 157.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (137, 168, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (186.11, 73.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686612 reads in 194.276 CPU sec, 6.420 real sec [M::mem_process_seqs] Processed 686890 reads in 128.547 CPU sec, 4.251 real sec [M::process] read 686032 sequences (100000182 bp) in 2.580 CPU sec, 2.737 real sec... sorting block 105, #reads = 1075781 mem = 536878203 peak = 1071730688 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 284538, 3, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 233, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (252.07, 116.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 300, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (320.54, 157.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 344, 561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1205) [M::mem_pestat] mean and std.dev: (406.26, 289.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1562) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 106, #reads = 1077036 mem = 536881488 peak = 1071730688 [M::process] read 684606 sequences (100000264 bp) in 3.105 CPU sec, 3.732 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 284178, 10, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 171, 2155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6147) [M::mem_pestat] mean and std.dev: (1074.00, 1872.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 305, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (325.14, 160.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1244, 1485, 3330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7502) [M::mem_pestat] mean and std.dev: (2191.80, 1106.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9588) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 497, 550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1226) [M::mem_pestat] mean and std.dev: (422.49, 198.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1564) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686750 reads in 196.114 CPU sec, 6.389 real sec [M::mem_process_seqs] Processed 686032 reads in 129.422 CPU sec, 4.193 real sec [M::process] read 682048 sequences (100000250 bp) in 2.483 CPU sec, 2.800 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 283185, 5, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 240, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1112) [M::mem_pestat] mean and std.dev: (291.00, 143.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1428) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 313, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (331.31, 161.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 254, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (278.81, 140.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 107, #reads = 1076328 mem = 536879930 peak = 1071730688 [M::process] read 684844 sequences (100000078 bp) in 2.841 CPU sec, 3.207 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 281862, 7, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 272, 566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1388) [M::mem_pestat] mean and std.dev: (350.29, 229.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 332, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960) [M::mem_pestat] mean and std.dev: (348.30, 167.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1209) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 279, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1095) [M::mem_pestat] mean and std.dev: (323.60, 210.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1401) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684606 reads in 199.079 CPU sec, 6.568 real sec [M::mem_process_seqs] Processed 682048 reads in 129.230 CPU sec, 4.264 real sec [M::process] read 684422 sequences (100000272 bp) in 2.907 CPU sec, 3.278 real sec... sorting block 108, #reads = 1074060 mem = 536879751 peak = 1071730688 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283755, 7, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 213, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (247.12, 146.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 314, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (333.39, 163.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1184) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 305, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (282.65, 126.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683490 sequences (100000266 bp) in 2.452 CPU sec, 2.694 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 283133, 4, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 323, 542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1252) [M::mem_pestat] mean and std.dev: (285.21, 147.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 315, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937) [M::mem_pestat] mean and std.dev: (335.61, 164.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1182) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 245, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (256.36, 135.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684844 reads in 197.954 CPU sec, 6.451 real sec [M::mem_process_seqs] Processed 684422 reads in 133.192 CPU sec, 4.311 real sec [M::process] read 683594 sequences (100000005 bp) in 2.614 CPU sec, 3.056 real sec... sorting block 109, #reads = 1073791 mem = 536877865 peak = 1071730688 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 283564, 2, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 244, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (265.16, 128.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 321, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947) [M::mem_pestat] mean and std.dev: (340.08, 166.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1194) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 289, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945) [M::mem_pestat] mean and std.dev: (303.53, 134.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1188) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683450 sequences (100000053 bp) in 2.883 CPU sec, 3.503 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 282681, 8, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (239, 301, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (309.34, 126.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 318, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936) [M::mem_pestat] mean and std.dev: (337.15, 164.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1180) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 369, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1261) [M::mem_pestat] mean and std.dev: (368.05, 221.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1612) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683490 reads in 194.389 CPU sec, 6.335 real sec [M::mem_process_seqs] Processed 683594 reads in 132.793 CPU sec, 4.291 real sec sorting block 110, #reads = 1074426 mem = 536878436 peak = 1071730688 [M::process] read 682362 sequences (100000036 bp) in 2.947 CPU sec, 3.193 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282855, 7, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 339, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (290.71, 105.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 319, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943) [M::mem_pestat] mean and std.dev: (338.67, 165.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1189) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (131, 216, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (211.54, 107.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 111, #reads = 1075563 mem = 536880887 peak = 1071730688 [M::process] read 681162 sequences (100000018 bp) in 2.920 CPU sec, 3.290 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 282542, 8, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 255, 515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1177) [M::mem_pestat] mean and std.dev: (288.59, 167.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 326, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950) [M::mem_pestat] mean and std.dev: (344.09, 166.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1197) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 280, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (327.44, 162.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683450 reads in 192.382 CPU sec, 6.287 real sec [M::mem_process_seqs] Processed 682362 reads in 129.461 CPU sec, 4.162 real sec [M::process] read 683580 sequences (100000151 bp) in 3.197 CPU sec, 3.505 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 282299, 7, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 270, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950) [M::mem_pestat] mean and std.dev: (319.16, 207.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1215) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 335, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969) [M::mem_pestat] mean and std.dev: (351.92, 169.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1220) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 250, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1319) [M::mem_pestat] mean and std.dev: (374.28, 304.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1699) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 112, #reads = 1074508 mem = 536878027 peak = 1071730688 [M::process] read 683950 sequences (100000021 bp) in 2.181 CPU sec, 2.725 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 282812, 18, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 215, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (255.05, 124.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 318, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943) [M::mem_pestat] mean and std.dev: (338.72, 166.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1189) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (302, 651, 1186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2954) [M::mem_pestat] mean and std.dev: (865.88, 704.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3838) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 261, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (289.07, 150.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681162 reads in 186.094 CPU sec, 6.139 real sec [M::mem_process_seqs] Processed 683580 reads in 125.340 CPU sec, 4.118 real sec sorting block 113, #reads = 1073919 mem = 536880949 peak = 1071730688 [M::process] read 683128 sequences (100000041 bp) in 3.602 CPU sec, 3.933 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283672, 12, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 290, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (299.11, 156.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 315, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (336.09, 165.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1179) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (92, 1221, 1349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3863) [M::mem_pestat] mean and std.dev: (810.17, 790.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5120) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 334, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (334.42, 178.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 282412, 1, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 274, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1310) [M::mem_pestat] mean and std.dev: (348.56, 243.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1696) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 320, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (340.45, 166.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1190) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 401, 638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1550) [M::mem_pestat] mean and std.dev: (440.63, 282.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2006) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683142 sequences (100000041 bp) in 2.762 CPU sec, 3.163 real sec... [M::mem_process_seqs] Processed 683950 reads in 186.537 CPU sec, 6.180 real sec [M::mem_process_seqs] Processed 683128 reads in 124.420 CPU sec, 4.128 real sec sorting block 114, #reads = 1076705 mem = 536877500 peak = 1071730688 [M::process] read 683822 sequences (100000022 bp) in 3.039 CPU sec, 3.306 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282524, 12, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 266, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1178) [M::mem_pestat] mean and std.dev: (311.12, 229.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 319, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958) [M::mem_pestat] mean and std.dev: (341.49, 169.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1209) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (188, 2932, 7712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 22760) [M::mem_pestat] mean and std.dev: (3311.58, 3244.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 30284) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 372, 562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1212) [M::mem_pestat] mean and std.dev: (343.38, 149.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1537) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683610 sequences (100000172 bp) in 2.054 CPU sec, 2.587 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 283271, 6, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 309, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029) [M::mem_pestat] mean and std.dev: (301.15, 154.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1300) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 316, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953) [M::mem_pestat] mean and std.dev: (339.19, 168.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1203) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (251, 430, 576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1226) [M::mem_pestat] mean and std.dev: (390.30, 202.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1551) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683142 reads in 191.543 CPU sec, 6.405 real sec sorting block 115, #reads = 1076926 mem = 536877377 peak = 1071730688 [M::mem_process_seqs] Processed 683822 reads in 126.900 CPU sec, 4.200 real sec [M::process] read 684512 sequences (100000234 bp) in 2.942 CPU sec, 3.236 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282743, 6, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 284, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1276) [M::mem_pestat] mean and std.dev: (386.74, 296.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 316, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950) [M::mem_pestat] mean and std.dev: (339.45, 168.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1199) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (255, 331, 709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1617) [M::mem_pestat] mean and std.dev: (438.40, 329.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2071) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 116, #reads = 1075874 mem = 536885272 peak = 1071730688 [M::process] read 688040 sequences (100000107 bp) in 2.580 CPU sec, 2.847 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 282918, 3, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 354, 1232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3382) [M::mem_pestat] mean and std.dev: (620.59, 713.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 311, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (334.56, 166.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1188) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 259, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (274.47, 136.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683610 reads in 213.372 CPU sec, 6.923 real sec [M::process] read 686434 sequences (100000107 bp) in 2.006 CPU sec, 2.061 real sec... [M::mem_process_seqs] Processed 684512 reads in 151.893 CPU sec, 4.911 real sec sorting block 117, #reads = 1072736 mem = 536880671 peak = 1071730688 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 285091, 7, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 238, 963) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2629) [M::mem_pestat] mean and std.dev: (540.24, 581.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 295, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (316.31, 156.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 320, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075) [M::mem_pestat] mean and std.dev: (341.14, 170.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1362) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686344 sequences (100000055 bp) in 2.584 CPU sec, 2.958 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 283947, 3, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 313, 1478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4002) [M::mem_pestat] mean and std.dev: (717.26, 603.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5264) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 305, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (325.39, 159.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1151) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (141, 358, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1071) [M::mem_pestat] mean and std.dev: (353.29, 259.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1391) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688040 reads in 191.034 CPU sec, 6.198 real sec [M::process] read 685580 sequences (100000102 bp) in 1.935 CPU sec, 1.972 real sec... [M::mem_process_seqs] Processed 686434 reads in 122.313 CPU sec, 3.970 real sec sorting block 118, #reads = 1068873 mem = 536878674 peak = 1071730688 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 284124, 4, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 217, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (226.88, 89.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 305, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (325.40, 160.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1151) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (252, 428, 584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1248) [M::mem_pestat] mean and std.dev: (453.45, 274.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1580) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685666 sequences (100000135 bp) in 2.781 CPU sec, 3.220 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 283712, 5, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 186, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810) [M::mem_pestat] mean and std.dev: (274.03, 201.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1081) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 310, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (328.00, 159.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 450, 1989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5579) [M::mem_pestat] mean and std.dev: (797.23, 759.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7374) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686344 reads in 180.733 CPU sec, 5.865 real sec [M::process] read 684468 sequences (100000037 bp) in 1.924 CPU sec, 1.997 real sec... [M::mem_process_seqs] Processed 685580 reads in 117.409 CPU sec, 3.789 real sec sorting block 119, #reads = 1077916 mem = 536879376 peak = 1071730688 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 283738, 5, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 269, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1263) [M::mem_pestat] mean and std.dev: (306.52, 187.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 311, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (329.87, 161.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1166) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 304, 1521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4181) [M::mem_pestat] mean and std.dev: (868.49, 859.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5511) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 120, #reads = 1079049 mem = 536879773 peak = 1071730688 [M::process] read 682106 sequences (100000169 bp) in 2.822 CPU sec, 3.160 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 284063, 13, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 239, 599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1509) [M::mem_pestat] mean and std.dev: (331.27, 262.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1964) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 317, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (334.62, 162.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1178) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1481, 1481, 1481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1481, 1481) [M::mem_pestat] mean and std.dev: (1481.00, 0.00) [M::mem_pestat] low and high boundaries for proper pairs: (1481, 1481) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 266, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020) [M::mem_pestat] mean and std.dev: (264.00, 100.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1296) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685666 reads in 182.678 CPU sec, 5.950 real sec [M::mem_process_seqs] Processed 684468 reads in 116.481 CPU sec, 3.759 real sec [M::process] read 685230 sequences (100000287 bp) in 2.254 CPU sec, 2.535 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 283012, 9, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 250, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (225.71, 112.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 333, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937) [M::mem_pestat] mean and std.dev: (345.22, 162.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1178) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 286, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1085) [M::mem_pestat] mean and std.dev: (345.48, 180.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1383) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 121, #reads = 1079280 mem = 536878922 peak = 1071730688 [M::process] read 686276 sequences (100000153 bp) in 3.071 CPU sec, 3.412 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 283628, 10, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 246, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961) [M::mem_pestat] mean and std.dev: (292.36, 151.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1210) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 311, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (329.39, 160.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1159) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (461, 476, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (309, 689) [M::mem_pestat] mean and std.dev: (491.29, 29.35) [M::mem_pestat] low and high boundaries for proper pairs: (233, 765) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 365, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010) [M::mem_pestat] mean and std.dev: (352.27, 179.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1286) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682106 reads in 184.806 CPU sec, 5.997 real sec [M::mem_process_seqs] Processed 685230 reads in 123.475 CPU sec, 3.960 real sec [M::process] read 686674 sequences (100000159 bp) in 2.448 CPU sec, 2.648 real sec... sorting block 122, #reads = 1077747 mem = 536876225 peak = 1071730688 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 284222, 3, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 192, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (242.87, 125.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 303, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (321.27, 156.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 292, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (287.06, 150.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686530 sequences (100000180 bp) in 2.556 CPU sec, 2.937 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 284367, 11, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 226, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961) [M::mem_pestat] mean and std.dev: (285.73, 233.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1235) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 300, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (318.36, 154.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 695, 2600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7770) [M::mem_pestat] mean and std.dev: (1033.73, 1085.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10355) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 234, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951) [M::mem_pestat] mean and std.dev: (271.33, 155.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1196) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686276 reads in 185.286 CPU sec, 6.063 real sec [M::mem_process_seqs] Processed 686674 reads in 123.885 CPU sec, 3.983 real sec sorting block 123, #reads = 1079243 mem = 536877571 peak = 1071730688 [M::process] read 685632 sequences (100000003 bp) in 2.347 CPU sec, 2.522 real sec... [M::process] read 685380 sequences (100000005 bp) in 2.632 CPU sec, 3.020 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 283310, 2, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 242, 1121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3071) [M::mem_pestat] mean and std.dev: (470.69, 443.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4046) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 305, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (323.35, 156.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 286, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (308.90, 138.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685632 reads in 104.960 CPU sec, 3.412 real sec sorting block 124, #reads = 1078231 mem = 536880482 peak = 1071730688 [M::process] read 685442 sequences (100000101 bp) in 2.915 CPU sec, 3.205 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 283842, 3, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 257, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973) [M::mem_pestat] mean and std.dev: (287.40, 202.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1246) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 307, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (325.69, 157.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 265, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (249.36, 79.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 283973, 14, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (239, 313, 557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1193) [M::mem_pestat] mean and std.dev: (385.16, 214.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 301, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (319.84, 155.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (615, 1167, 1256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2538) [M::mem_pestat] mean and std.dev: (876.62, 352.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3179) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 295, 550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1276) [M::mem_pestat] mean and std.dev: (338.14, 200.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1639) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685380 reads in 123.415 CPU sec, 4.064 real sec [M::mem_process_seqs] Processed 686530 reads in 310.443 CPU sec, 10.212 real sec [M::process] read 683514 sequences (100000234 bp) in 2.210 CPU sec, 2.399 real sec... sorting block 125, #reads = 1078754 mem = 536878821 peak = 1071730688 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 283566, 11, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 180, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (161.92, 51.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 310, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (327.87, 159.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (155, 1052, 1227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3371) [M::mem_pestat] mean and std.dev: (744.50, 612.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4443) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (272, 534, 917) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2207) [M::mem_pestat] mean and std.dev: (691.06, 565.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2953) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683020 sequences (100000007 bp) in 2.663 CPU sec, 3.028 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 282627, 8, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 327, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999) [M::mem_pestat] mean and std.dev: (287.81, 147.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1281) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 320, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931) [M::mem_pestat] mean and std.dev: (336.81, 161.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1173) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (267, 364, 835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1971) [M::mem_pestat] mean and std.dev: (516.70, 398.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2539) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685442 reads in 188.840 CPU sec, 6.171 real sec [M::mem_process_seqs] Processed 683514 reads in 125.260 CPU sec, 4.112 real sec sorting block 126, #reads = 1077861 mem = 536877942 peak = 1071730688 [M::process] read 685194 sequences (100000048 bp) in 2.164 CPU sec, 2.387 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282823, 9, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 202, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (209.59, 112.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 324, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931) [M::mem_pestat] mean and std.dev: (339.46, 161.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (256, 264, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (6, 631) [M::mem_pestat] mean and std.dev: (313.73, 110.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 127, #reads = 1077804 mem = 536878206 peak = 1071730688 [M::process] read 684760 sequences (100000202 bp) in 2.910 CPU sec, 3.280 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 284083, 7, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 256, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055) [M::mem_pestat] mean and std.dev: (300.29, 175.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1357) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 308, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (326.58, 158.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (138, 221, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (246.33, 137.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683020 reads in 187.353 CPU sec, 6.137 real sec [M::mem_process_seqs] Processed 685194 reads in 120.695 CPU sec, 3.948 real sec [M::process] read 684762 sequences (100000201 bp) in 1.848 CPU sec, 2.024 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282752, 2, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 200, 690) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1772) [M::mem_pestat] mean and std.dev: (233.86, 149.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2313) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 310, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (328.58, 158.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 350, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (286.05, 87.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 128, #reads = 1076495 mem = 536881716 peak = 1071730688 [M::process] read 684300 sequences (100000084 bp) in 3.225 CPU sec, 3.584 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 282584, 11, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 168, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (184.35, 57.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 310, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (329.02, 159.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (619, 619, 691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (475, 835) [M::mem_pestat] mean and std.dev: (655.00, 35.02) [M::mem_pestat] low and high boundaries for proper pairs: (403, 907) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (273, 360, 732) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1650) [M::mem_pestat] mean and std.dev: (436.74, 266.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2109) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684760 reads in 190.349 CPU sec, 6.273 real sec [M::mem_process_seqs] Processed 684762 reads in 125.789 CPU sec, 4.143 real sec [M::process] read 684092 sequences (100000025 bp) in 2.136 CPU sec, 2.340 real sec... sorting block 129, #reads = 1078489 mem = 536878624 peak = 1071730688 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 283201, 4, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 235, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (222.93, 79.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 312, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (330.31, 159.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (277, 369, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027) [M::mem_pestat] mean and std.dev: (357.30, 144.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1277) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684100 sequences (100000043 bp) in 2.935 CPU sec, 3.283 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 283097, 2, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 226, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (235.33, 108.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1026) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 316, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (334.36, 162.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1171) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 356, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1150) [M::mem_pestat] mean and std.dev: (386.29, 224.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1458) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684300 reads in 192.642 CPU sec, 6.278 real sec [M::mem_process_seqs] Processed 684092 reads in 131.734 CPU sec, 4.260 real sec [M::process] read 684266 sequences (100000272 bp) in 2.055 CPU sec, 2.165 real sec... sorting block 130, #reads = 1076638 mem = 536882419 peak = 1071730688 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 283141, 14, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 320, 627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1433) [M::mem_pestat] mean and std.dev: (413.16, 304.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1836) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 316, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932) [M::mem_pestat] mean and std.dev: (334.78, 162.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1175) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (624, 1280, 1297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2643) [M::mem_pestat] mean and std.dev: (1001.92, 487.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3316) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (129, 239, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1167) [M::mem_pestat] mean and std.dev: (252.56, 191.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1513) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684382 sequences (100000081 bp) in 3.127 CPU sec, 3.533 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 283724, 6, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 294, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027) [M::mem_pestat] mean and std.dev: (322.60, 197.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1300) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 314, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (334.62, 163.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1178) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 277, 558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1298) [M::mem_pestat] mean and std.dev: (383.94, 303.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1668) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684100 reads in 194.945 CPU sec, 6.323 real sec sorting block 131, #reads = 1075922 mem = 536879935 peak = 1071730688 [M::mem_process_seqs] Processed 684266 reads in 132.130 CPU sec, 4.255 real sec [M::process] read 684302 sequences (100000209 bp) in 1.920 CPU sec, 2.134 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 283353, 8, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 239, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (254.23, 126.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 314, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (334.83, 164.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1185) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 274, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (317.32, 173.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1075) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 132, #reads = 1076227 mem = 536880273 peak = 1071730688 [M::process] read 684154 sequences (100000038 bp) in 3.192 CPU sec, 3.556 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 282732, 9, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 223, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1276) [M::mem_pestat] mean and std.dev: (288.98, 172.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 313, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (334.75, 164.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1185) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 279, 699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1697) [M::mem_pestat] mean and std.dev: (360.22, 229.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2196) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684382 reads in 198.429 CPU sec, 6.505 real sec [M::mem_process_seqs] Processed 684302 reads in 134.216 CPU sec, 4.396 real sec [M::process] read 684894 sequences (100000057 bp) in 1.975 CPU sec, 2.162 real sec... sorting block 133, #reads = 1074671 mem = 536878647 peak = 1071730688 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 282814, 4, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (238, 319, 664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1516) [M::mem_pestat] mean and std.dev: (392.04, 235.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1942) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 315, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (336.52, 165.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1194) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 289, 613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1451) [M::mem_pestat] mean and std.dev: (440.52, 341.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1870) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684982 sequences (100000276 bp) in 2.769 CPU sec, 3.098 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 283884, 8, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 262, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (245.03, 86.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 308, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (330.82, 163.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1170) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 259, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (277.35, 144.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684154 reads in 195.519 CPU sec, 6.344 real sec [M::process] read 685264 sequences (100000146 bp) in 1.813 CPU sec, 1.821 real sec... [M::mem_process_seqs] Processed 684894 reads in 132.236 CPU sec, 4.262 real sec sorting block 134, #reads = 1074246 mem = 536877155 peak = 1071730688 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 283361, 5, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 300, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046) [M::mem_pestat] mean and std.dev: (384.25, 252.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1393) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 306, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (329.16, 163.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1158) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (282, 357, 639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1353) [M::mem_pestat] mean and std.dev: (473.29, 288.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1710) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 690786 sequences (100000074 bp) in 3.065 CPU sec, 3.545 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 283156, 5, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 249, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1137) [M::mem_pestat] mean and std.dev: (316.13, 204.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 305, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (328.20, 163.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (138, 255, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1236) [M::mem_pestat] mean and std.dev: (302.74, 185.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1602) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688408 sequences (100000070 bp) in 1.914 CPU sec, 1.917 real sec... [M::mem_process_seqs] Processed 684982 reads in 210.694 CPU sec, 6.803 real sec sorting block 135, #reads = 1074383 mem = 536879564 peak = 1071730688 [M::mem_process_seqs] Processed 685264 reads in 147.784 CPU sec, 4.749 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 285541, 11, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 224, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (236.59, 90.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 286, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (307.44, 151.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (979, 979, 5434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14344) [M::mem_pestat] mean and std.dev: (2264.36, 1956.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18799) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 252, 697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1753) [M::mem_pestat] mean and std.dev: (333.41, 249.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2281) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 136, #reads = 1068811 mem = 536884398 peak = 1071730688 [M::process] read 688354 sequences (100000107 bp) in 3.141 CPU sec, 3.458 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 284504, 2, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 320, 549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1263) [M::mem_pestat] mean and std.dev: (296.13, 158.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 298, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (315.68, 152.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 303, 551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1257) [M::mem_pestat] mean and std.dev: (357.38, 205.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1610) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689460 sequences (100000131 bp) in 1.890 CPU sec, 2.051 real sec... [M::mem_process_seqs] Processed 690786 reads in 215.524 CPU sec, 6.946 real sec [M::mem_process_seqs] Processed 688408 reads in 141.621 CPU sec, 4.555 real sec sorting block 137, #reads = 1070865 mem = 536878329 peak = 1071730688 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 285236, 5, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 256, 996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2638) [M::mem_pestat] mean and std.dev: (549.89, 607.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 295, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (314.15, 152.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (246, 311, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (284.10, 90.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686892 sequences (100000172 bp) in 3.418 CPU sec, 3.826 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 285319, 11, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 261, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (251.26, 116.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 287, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (307.71, 149.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1089) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (194, 194, 1556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4280) [M::mem_pestat] mean and std.dev: (730.18, 846.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5642) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 217, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1063) [M::mem_pestat] mean and std.dev: (288.90, 164.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1360) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688354 reads in 201.688 CPU sec, 6.479 real sec [M::process] read 685114 sequences (100000048 bp) in 1.814 CPU sec, 1.842 real sec... [M::mem_process_seqs] Processed 689460 reads in 121.044 CPU sec, 3.851 real sec sorting block 138, #reads = 1071057 mem = 536880365 peak = 1071730688 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 283685, 7, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 220, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (215.63, 101.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 303, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (320.16, 154.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 281, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (294.10, 128.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1083) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688462 sequences (100000001 bp) in 2.644 CPU sec, 2.751 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 283639, 15, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 247, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (261.80, 141.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 318, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (331.81, 157.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1173) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (151, 1799, 1830) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5188) [M::mem_pestat] mean and std.dev: (1317.53, 887.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6867) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 335, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029) [M::mem_pestat] mean and std.dev: (296.61, 153.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1313) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688302 sequences (100000044 bp) in 1.490 CPU sec, 1.497 real sec... [M::mem_process_seqs] Processed 686892 reads in 151.563 CPU sec, 4.807 real sec sorting block 139, #reads = 1073150 mem = 536876880 peak = 1071730688 [M::mem_process_seqs] Processed 685114 reads in 97.165 CPU sec, 3.076 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 285116, 7, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 245, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (264.30, 146.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 293, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (313.70, 153.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 244, 632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1516) [M::mem_pestat] mean and std.dev: (420.63, 349.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1958) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689176 sequences (100000152 bp) in 3.133 CPU sec, 3.293 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 284956, 4, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 212, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (191.15, 54.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 294, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (315.33, 155.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 388, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1106) [M::mem_pestat] mean and std.dev: (289.44, 126.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1408) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688474 sequences (100000051 bp) in 1.889 CPU sec, 1.895 real sec... [M::mem_process_seqs] Processed 688462 reads in 182.013 CPU sec, 5.778 real sec sorting block 140, #reads = 1072835 mem = 536878066 peak = 1071730688 [M::mem_process_seqs] Processed 688302 reads in 124.008 CPU sec, 3.943 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 285177, 5, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 210, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (267.58, 143.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 288, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (308.93, 151.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 231, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (319.20, 209.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 141, #reads = 1076053 mem = 536879110 peak = 1084207104 [M::process] read 687606 sequences (100000198 bp) in 2.967 CPU sec, 3.192 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 284790, 5, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 298, 554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1216) [M::mem_pestat] mean and std.dev: (337.53, 202.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 290, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (308.07, 148.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1072) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 266, 868) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2230) [M::mem_pestat] mean and std.dev: (413.13, 437.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2911) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687106 sequences (100000226 bp) in 1.829 CPU sec, 1.863 real sec... [M::mem_process_seqs] Processed 689176 reads in 196.790 CPU sec, 6.312 real sec [M::mem_process_seqs] Processed 688474 reads in 130.945 CPU sec, 4.157 real sec sorting block 142, #reads = 1076089 mem = 536880150 peak = 1084207104 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 284585, 10, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 213, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042) [M::mem_pestat] mean and std.dev: (281.92, 183.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1341) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 295, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (313.30, 151.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1091) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1274, 1368, 2536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5060) [M::mem_pestat] mean and std.dev: (1493.40, 788.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6322) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 292, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (265.91, 136.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684316 sequences (100000060 bp) in 3.234 CPU sec, 3.419 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 284333, 5, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 219, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018) [M::mem_pestat] mean and std.dev: (279.41, 151.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1299) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 299, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (316.62, 152.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 848, 2091) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5887) [M::mem_pestat] mean and std.dev: (926.66, 1047.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7785) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687606 reads in 179.761 CPU sec, 5.716 real sec [M::process] read 685200 sequences (100000277 bp) in 2.073 CPU sec, 2.100 real sec... [M::mem_process_seqs] Processed 687106 reads in 120.219 CPU sec, 3.796 real sec sorting block 143, #reads = 1075426 mem = 536878139 peak = 1084207104 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 283660, 6, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 258, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (292.52, 150.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 315, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (329.26, 155.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (268, 365, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (308.75, 105.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686076 sequences (100000154 bp) in 2.511 CPU sec, 2.632 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 283443, 5, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 351, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (318.48, 153.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 307, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (323.24, 153.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 230, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (244.89, 107.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686096 sequences (100000214 bp) in 1.918 CPU sec, 1.926 real sec... [M::mem_process_seqs] Processed 684316 reads in 180.813 CPU sec, 5.774 real sec sorting block 144, #reads = 1076331 mem = 536881371 peak = 1084207104 [M::mem_process_seqs] Processed 685200 reads in 122.690 CPU sec, 3.923 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 284071, 9, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 241, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (216.62, 73.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 303, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (320.69, 153.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 324, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (315.65, 165.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1070) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 145, #reads = 1075044 mem = 536877490 peak = 1084207104 [M::process] read 685074 sequences (100000010 bp) in 3.127 CPU sec, 3.290 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 283656, 3, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 267, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1105) [M::mem_pestat] mean and std.dev: (266.82, 181.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 302, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (319.60, 153.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 320, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1158) [M::mem_pestat] mean and std.dev: (307.71, 146.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1483) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686076 reads in 189.131 CPU sec, 6.060 real sec [M::process] read 685566 sequences (100000029 bp) in 1.872 CPU sec, 1.903 real sec... [M::mem_process_seqs] Processed 686096 reads in 125.365 CPU sec, 3.979 real sec sorting block 146, #reads = 1077125 mem = 536878851 peak = 1084207104 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 283953, 5, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 320, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052) [M::mem_pestat] mean and std.dev: (341.10, 175.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1333) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 310, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (327.32, 156.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (284, 339, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (66, 611) [M::mem_pestat] mean and std.dev: (313.92, 109.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685310 sequences (100000266 bp) in 2.846 CPU sec, 3.057 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 283445, 5, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 280, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (258.62, 107.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 305, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (322.57, 154.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 324, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (296.35, 91.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684844 sequences (100000160 bp) in 1.924 CPU sec, 1.932 real sec... [M::mem_process_seqs] Processed 685074 reads in 190.123 CPU sec, 6.091 real sec [M::mem_process_seqs] Processed 685566 reads in 124.930 CPU sec, 3.978 real sec sorting block 147, #reads = 1076264 mem = 536878257 peak = 1084207104 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 282890, 5, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 265, 666) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1696) [M::mem_pestat] mean and std.dev: (315.65, 259.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2211) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 307, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (325.19, 156.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 321, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1188) [M::mem_pestat] mean and std.dev: (360.18, 249.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1527) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684586 sequences (100000244 bp) in 3.240 CPU sec, 3.451 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 283029, 19, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 345, 546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1298) [M::mem_pestat] mean and std.dev: (366.23, 275.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 310, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (328.36, 158.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (368, 368, 602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1070) [M::mem_pestat] mean and std.dev: (402.22, 260.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1446) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 310, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (270.78, 94.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685310 reads in 189.903 CPU sec, 6.086 real sec [M::process] read 684538 sequences (100000024 bp) in 2.009 CPU sec, 2.045 real sec... [M::mem_process_seqs] Processed 684844 reads in 126.277 CPU sec, 4.007 real sec sorting block 148, #reads = 1077224 mem = 536879455 peak = 1084207104 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 283019, 12, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 233, 599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1517) [M::mem_pestat] mean and std.dev: (331.90, 264.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1976) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 308, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (326.61, 156.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (859, 1451, 2405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5497) [M::mem_pestat] mean and std.dev: (1360.08, 706.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7043) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 296, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804) [M::mem_pestat] mean and std.dev: (277.67, 112.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684618 sequences (100000275 bp) in 2.922 CPU sec, 3.267 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 283141, 6, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 223, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (239.24, 144.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 310, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (328.81, 159.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 262, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947) [M::mem_pestat] mean and std.dev: (290.13, 163.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1208) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684586 reads in 189.275 CPU sec, 6.085 real sec sorting block 149, #reads = 1076836 mem = 536884909 peak = 1084207104 [M::mem_process_seqs] Processed 684538 reads in 126.784 CPU sec, 4.016 real sec [M::process] read 684454 sequences (100000179 bp) in 2.342 CPU sec, 2.409 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 283251, 7, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 496, 860) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2146) [M::mem_pestat] mean and std.dev: (563.64, 421.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 309, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (328.98, 159.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 241, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (264.73, 129.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 150, #reads = 1075953 mem = 536880057 peak = 1084207104 [M::process] read 684240 sequences (100000166 bp) in 3.013 CPU sec, 3.262 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 283264, 15, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 238, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (238.40, 92.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 311, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921) [M::mem_pestat] mean and std.dev: (331.05, 160.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (386, 729, 1305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3143) [M::mem_pestat] mean and std.dev: (676.50, 540.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4062) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 257, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804) [M::mem_pestat] mean and std.dev: (259.18, 112.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684618 reads in 187.632 CPU sec, 6.023 real sec [M::mem_process_seqs] Processed 684454 reads in 122.051 CPU sec, 3.865 real sec [M::process] read 683936 sequences (100000283 bp) in 2.448 CPU sec, 2.537 real sec... sorting block 151, #reads = 1076938 mem = 536880196 peak = 1084207104 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283164, 7, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 297, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1186) [M::mem_pestat] mean and std.dev: (284.25, 169.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 313, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (332.69, 162.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1169) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 253, 679) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1625) [M::mem_pestat] mean and std.dev: (378.95, 266.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2098) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 428288 sequences (62445638 bp) in 1.863 CPU sec, 1.981 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 283487, 6, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 228, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006) [M::mem_pestat] mean and std.dev: (257.92, 128.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1294) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 312, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (333.64, 162.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1167) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (321, 405, 789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1725) [M::mem_pestat] mean and std.dev: (405.44, 205.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2193) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684240 reads in 151.786 CPU sec, 4.906 real sec [M::mem_process_seqs] Processed 683936 reads in 92.752 CPU sec, 2.980 real sec sorting block 152, #reads = 1076352 mem = 536882685 peak = 1084207104 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 177136, 14, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 234, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (254.78, 123.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 302, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (325.45, 162.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1151) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1006, 1512, 1822) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3454) [M::mem_pestat] mean and std.dev: (1288.69, 496.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4270) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 363, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (289.00, 119.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 995) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 428288 reads in 51.306 CPU sec, 1.702 real sec sorting block 153, #reads = 1076588 mem = 536879308 peak = 1084207104 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 165225120 overall: 69987926016 mem 26049.614 user 556.001 sys 906.118 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_7OW-T1-TDNA-1_A23MHWWLT4_1.L008\tPU:L008\tSM:1136_7OW-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_7OW-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1136_7OW-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 1136_7OW-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 906.153 sec; CPU: 26605.618 sec sorting block 154, #reads = 190387 mem = 95190509 peak = 1084207104 uniq header count 1 merging 155 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 9002951383 output reads: 166147502 bam_mem_sort: 155 calls 106.354 user 2.733 sys 112.758 real bam_write: 155 calls 95.698 user 7.356 sys 204.359 real execute: 1 calls 38.776 user 1.923 sys 957.578 real merge_files: 1 calls 38.747 user 1.905 sys 51.288 real parse_chunk: 2467 calls 111.702 user 31.230 sys 181.741 real read_chunk: 6655 calls 53.726 user 40.847 sys 853.521 real sort_block: 1 calls 38.268 user 40.240 sys 831.826 real write_chunk: 4186 calls 38.267 user 1.446 sys 50.098 real overall: 3310034944 mem 2097.537 user 105.709 sys 957.598 real ls: cannot access '*.cram': No such file or directory