.command.err - Workdir - DAQ:SENTIEON_BWAMEM (659_d0M-T1-TDNA-1_A23MHWWLT4_1;L001;0001)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ce/5aac953c2db0669de84af60224850e/.command.err
Size: 203.7 KB
Task: DAQ:SENTIEON_BWAMEM (659_d0M-T1-TDNA-1_A23MHWWLT4_1;L001;0001)
Attempt
Content

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_d0M-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1757.055 CPU sec, 71.693 real sec
[M::process] read 676862 sequences (100000021 bp) in 0.948 CPU sec, 0.954 real sec...
[M::process] read 676534 sequences (100000172 bp) in 1.092 CPU sec, 1.105 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284963, 4, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 168, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423)
[M::mem_pestat] mean and std.dev: (213.40, 90.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 577)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 325, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (344.53, 156.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 676144 sequences (100000109 bp) in 1.107 CPU sec, 1.123 real sec...
[M::process] read 675726 sequences (100000197 bp) in 1.097 CPU sec, 1.109 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284478, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 328, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (346.50, 157.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676862 reads in 143.433 CPU sec, 4.586 real sec
[M::mem_process_seqs] Processed 676534 reads in 88.193 CPU sec, 2.850 real sec
sorting block 0, #reads = 1080416 mem = 536878906 peak = 605507584
[M::process] read 675650 sequences (100000116 bp) in 1.664 CPU sec, 1.762 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285085, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 334, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (351.45, 159.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675458 sequences (100000027 bp) in 1.180 CPU sec, 1.175 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284129, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 337, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (355.12, 161.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 284221, 13, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (1586, 1586, 2858)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5402)
[M::mem_pestat] mean and std.dev: (1680.29, 781.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6674)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 339, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (356.21, 161.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (36, 1822, 2265)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6723)
[M::mem_pestat] mean and std.dev: (1419.46, 976.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8952)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676144 reads in 193.033 CPU sec, 6.191 real sec
[M::mem_process_seqs] Processed 675726 reads in 143.031 CPU sec, 4.615 real sec
[M::mem_process_seqs] Processed 675650 reads in 155.304 CPU sec, 5.029 real sec
sorting block 1, #reads = 1081289 mem = 536879265 peak = 734482432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284792, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 341, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (357.84, 161.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675132 sequences (100000020 bp) in 2.163 CPU sec, 2.383 real sec...
sorting block 2, #reads = 1080469 mem = 536882464 peak = 792391680
[M::mem_process_seqs] Processed 675458 reads in 78.088 CPU sec, 2.556 real sec
[M::process] read 675030 sequences (100000197 bp) in 1.814 CPU sec, 1.957 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284601, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 341, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (358.07, 161.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674732 sequences (100000123 bp) in 1.220 CPU sec, 1.227 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284771, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 344, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (360.68, 162.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674858 sequences (100000298 bp) in 1.347 CPU sec, 1.355 real sec...
[M::mem_process_seqs] Processed 675132 reads in 138.591 CPU sec, 4.374 real sec
sorting block 3, #reads = 1080871 mem = 536879751 peak = 792391680
[M::mem_process_seqs] Processed 675030 reads in 86.172 CPU sec, 2.723 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284236, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 347, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (363.53, 163.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 4, #reads = 1081239 mem = 536877966 peak = 793464832
[M::process] read 675606 sequences (100000029 bp) in 1.952 CPU sec, 2.092 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283809, 4, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 349, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (364.57, 163.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (88, 129, 216)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472)
[M::mem_pestat] mean and std.dev: (134.08, 58.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 600)
[M::mem_pestat] skip orientation RR
[M::process] read 675694 sequences (100000068 bp) in 1.360 CPU sec, 1.363 real sec...
[M::mem_process_seqs] Processed 674732 reads in 140.681 CPU sec, 4.518 real sec
[M::mem_process_seqs] Processed 674858 reads in 88.660 CPU sec, 2.811 real sec
sorting block 5, #reads = 1081044 mem = 536877021 peak = 793464832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284124, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 339, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (356.99, 162.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675682 sequences (100000201 bp) in 2.292 CPU sec, 2.441 real sec...
[M::process] read 675594 sequences (100000177 bp) in 1.278 CPU sec, 1.286 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 284888, 1, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (541, 541, 541)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (541, 541)
[M::mem_pestat] mean and std.dev: (541.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (541, 541)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 338, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (356.86, 162.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 316, 828)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2100)
[M::mem_pestat] mean and std.dev: (476.31, 333.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2736)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675606 reads in 146.645 CPU sec, 4.665 real sec
[M::mem_process_seqs] Processed 675694 reads in 92.346 CPU sec, 2.930 real sec
sorting block 6, #reads = 1081067 mem = 536881044 peak = 793464832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284806, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 339, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (357.69, 162.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675432 sequences (100000284 bp) in 1.986 CPU sec, 2.180 real sec...
[M::process] read 675414 sequences (100000092 bp) in 1.280 CPU sec, 1.280 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284126, 6, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 273, 651)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1641)
[M::mem_pestat] mean and std.dev: (403.00, 397.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2136)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 339, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (357.23, 162.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675682 reads in 143.738 CPU sec, 4.601 real sec
[M::mem_process_seqs] Processed 675594 reads in 90.388 CPU sec, 2.887 real sec
sorting block 7, #reads = 1082583 mem = 536882002 peak = 793464832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283778, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 338, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (356.64, 162.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675256 sequences (100000272 bp) in 2.009 CPU sec, 2.155 real sec...
[M::process] read 675452 sequences (100000036 bp) in 1.547 CPU sec, 1.563 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284172, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 336, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (355.27, 161.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675432 reads in 152.114 CPU sec, 4.852 real sec
sorting block 8, #reads = 1081083 mem = 536878455 peak = 793464832
[M::mem_process_seqs] Processed 675414 reads in 97.601 CPU sec, 3.102 real sec
sorting block 9, #reads = 1082273 mem = 536878155 peak = 793464832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284094, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 337, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (355.22, 159.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675402 sequences (100000164 bp) in 2.000 CPU sec, 2.175 real sec...
[M::process] read 676046 sequences (100000157 bp) in 1.410 CPU sec, 1.411 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284256, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 334, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (352.60, 158.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675256 reads in 154.226 CPU sec, 4.928 real sec
[M::mem_process_seqs] Processed 675452 reads in 95.997 CPU sec, 3.057 real sec
sorting block 10, #reads = 1080872 mem = 536880302 peak = 793464832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284540, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 333, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (350.67, 156.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676188 sequences (100000143 bp) in 2.250 CPU sec, 2.400 real sec...
[M::process] read 675580 sequences (100000038 bp) in 1.420 CPU sec, 1.416 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284583, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 323, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (340.59, 151.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1091)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675402 reads in 157.256 CPU sec, 5.031 real sec
[M::mem_process_seqs] Processed 676046 reads in 102.053 CPU sec, 3.245 real sec
sorting block 11, #reads = 1082654 mem = 536878580 peak = 793464832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284546, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 323, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879)
[M::mem_pestat] mean and std.dev: (341.00, 152.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1098)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675482 sequences (100000259 bp) in 2.032 CPU sec, 2.237 real sec...
[M::process] read 675400 sequences (100000115 bp) in 1.570 CPU sec, 1.575 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284627, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 329, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (347.29, 155.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676188 reads in 161.587 CPU sec, 5.166 real sec
[M::mem_process_seqs] Processed 675580 reads in 98.053 CPU sec, 3.122 real sec
sorting block 12, #reads = 1077910 mem = 536882843 peak = 793464832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284091, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 328, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (347.77, 156.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675298 sequences (100000048 bp) in 1.999 CPU sec, 2.133 real sec...
[M::process] read 675224 sequences (100000143 bp) in 1.360 CPU sec, 1.359 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284131, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 330, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (348.76, 156.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675482 reads in 148.980 CPU sec, 4.750 real sec
sorting block 13, #reads = 1081237 mem = 536878655 peak = 793464832
[M::mem_process_seqs] Processed 675400 reads in 95.102 CPU sec, 3.023 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284441, 6, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 14, #reads = 1081733 mem = 536878410 peak = 851300352
[M::mem_pestat] (25, 50, 75) percentile: (226, 327, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (346.61, 155.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (315, 330, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (285, 360)
[M::mem_pestat] mean and std.dev: (327.86, 5.25)
[M::mem_pestat] low and high boundaries for proper pairs: (270, 375)
[M::mem_pestat] skip orientation RR
[M::process] read 675060 sequences (100000274 bp) in 2.092 CPU sec, 2.255 real sec...
[M::process] read 674998 sequences (100000143 bp) in 1.479 CPU sec, 1.493 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283980, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 331, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (349.40, 156.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675298 reads in 154.558 CPU sec, 4.927 real sec
[M::mem_process_seqs] Processed 675224 reads in 97.553 CPU sec, 3.093 real sec
sorting block 15, #reads = 1081219 mem = 536879341 peak = 851300352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284310, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 331, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (350.73, 157.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674970 sequences (100000179 bp) in 2.085 CPU sec, 2.248 real sec...
[M::process] read 674738 sequences (100000112 bp) in 1.396 CPU sec, 1.404 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284236, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 330, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (349.43, 156.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675060 reads in 153.545 CPU sec, 4.896 real sec
[M::mem_process_seqs] Processed 674998 reads in 97.678 CPU sec, 3.099 real sec
sorting block 16, #reads = 1081344 mem = 536880479 peak = 851300352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283689, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 332, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (350.59, 157.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675410 sequences (100000055 bp) in 2.148 CPU sec, 2.320 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284097, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 332, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (352.18, 157.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676048 sequences (100000132 bp) in 1.585 CPU sec, 1.591 real sec...
[M::mem_process_seqs] Processed 674970 reads in 156.909 CPU sec, 5.019 real sec
[M::mem_process_seqs] Processed 674738 reads in 102.140 CPU sec, 3.245 real sec
sorting block 17, #reads = 1080806 mem = 536876681 peak = 851300352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284619, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 331, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (350.36, 157.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675886 sequences (100000051 bp) in 2.285 CPU sec, 2.422 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284255, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 329, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (347.80, 157.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675776 sequences (100000224 bp) in 1.567 CPU sec, 1.568 real sec...
[M::mem_process_seqs] Processed 675410 reads in 155.463 CPU sec, 4.978 real sec
sorting block 18, #reads = 1080335 mem = 536877296 peak = 851300352
[M::mem_process_seqs] Processed 676048 reads in 99.010 CPU sec, 3.149 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284063, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 332, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (350.48, 158.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 19, #reads = 1081219 mem = 536877813 peak = 879955968
[M::process] read 675332 sequences (100000166 bp) in 2.178 CPU sec, 2.333 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284180, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 337, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (354.62, 161.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675460 sequences (100000072 bp) in 1.570 CPU sec, 1.560 real sec...
[M::mem_process_seqs] Processed 675886 reads in 155.075 CPU sec, 4.964 real sec
[M::mem_process_seqs] Processed 675776 reads in 99.885 CPU sec, 3.188 real sec
sorting block 20, #reads = 1081496 mem = 536879325 peak = 879955968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283951, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 341, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (358.52, 163.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675172 sequences (100000268 bp) in 2.262 CPU sec, 2.413 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284612, 1, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (222, 222, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (222, 222)
[M::mem_pestat] mean and std.dev: (222.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (222, 222)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 343, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (361.03, 164.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675332 reads in 151.587 CPU sec, 4.838 real sec
[M::process] read 674794 sequences (100000062 bp) in 1.618 CPU sec, 1.624 real sec...
[M::mem_process_seqs] Processed 675460 reads in 99.639 CPU sec, 3.153 real sec
sorting block 21, #reads = 1082644 mem = 536878032 peak = 879955968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283975, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 344, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (361.68, 164.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (397, 397, 688)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1270)
[M::mem_pestat] mean and std.dev: (508.00, 254.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1561)
[M::mem_pestat] skip orientation RR
[M::process] read 674852 sequences (100000260 bp) in 2.211 CPU sec, 2.350 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284587, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 346, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (362.31, 163.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674560 sequences (100000174 bp) in 1.708 CPU sec, 1.706 real sec...
[M::mem_process_seqs] Processed 675172 reads in 152.302 CPU sec, 4.850 real sec
[M::mem_process_seqs] Processed 674794 reads in 97.454 CPU sec, 3.088 real sec
sorting block 22, #reads = 1081567 mem = 536879440 peak = 879955968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284231, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 348, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (363.75, 163.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 406, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1145)
[M::mem_pestat] mean and std.dev: (327.40, 143.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1477)
[M::mem_pestat] skip orientation RR
[M::process] read 674568 sequences (100000280 bp) in 2.540 CPU sec, 2.680 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284339, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 353, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (368.89, 166.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674852 reads in 151.731 CPU sec, 4.861 real sec
[M::mem_process_seqs] Processed 674560 reads in 99.568 CPU sec, 3.146 real sec
sorting block 23, #reads = 1081221 mem = 536879755 peak = 879955968
[M::process] read 675144 sequences (100000194 bp) in 2.212 CPU sec, 2.297 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284848, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 352, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (367.63, 165.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 24, #reads = 1081439 mem = 536880687 peak = 889270272
[M::process] read 675410 sequences (100000059 bp) in 2.092 CPU sec, 2.246 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284399, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 344, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (361.39, 164.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674568 reads in 157.468 CPU sec, 5.035 real sec
[M::process] read 675470 sequences (100000193 bp) in 1.771 CPU sec, 1.789 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284373, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 341, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (358.90, 163.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675174 sequences (100000029 bp) in 1.624 CPU sec, 1.626 real sec...
[M::mem_process_seqs] Processed 675410 reads in 87.847 CPU sec, 2.982 real sec
sorting block 25, #reads = 1081535 mem = 536880346 peak = 889270272
[M::mem_process_seqs] Processed 675144 reads in 245.146 CPU sec, 7.982 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284290, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 340, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (358.46, 163.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284418, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 340, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (358.57, 162.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675386 sequences (100000090 bp) in 2.368 CPU sec, 2.529 real sec...
[M::mem_process_seqs] Processed 675470 reads in 164.120 CPU sec, 5.263 real sec
sorting block 26, #reads = 1082644 mem = 536878473 peak = 889270272
[M::mem_process_seqs] Processed 675174 reads in 99.631 CPU sec, 3.192 real sec
[M::process] read 675396 sequences (100000082 bp) in 2.455 CPU sec, 2.607 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284569, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 337, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (355.81, 162.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 27, #reads = 1082786 mem = 536880260 peak = 889270272
[M::process] read 675242 sequences (100000182 bp) in 2.143 CPU sec, 2.260 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284179, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 336, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (355.23, 161.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675386 reads in 158.766 CPU sec, 5.059 real sec
[M::mem_process_seqs] Processed 675396 reads in 103.334 CPU sec, 3.255 real sec
[M::process] read 675588 sequences (100000186 bp) in 2.282 CPU sec, 2.378 real sec...
sorting block 28, #reads = 1081863 mem = 536880896 peak = 889270272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284307, 1, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (400, 671, 671)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1213)
[M::mem_pestat] mean and std.dev: (550.56, 134.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1484)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 334, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (353.69, 160.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675830 sequences (100000058 bp) in 2.427 CPU sec, 2.568 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284319, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 331, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (350.98, 159.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675242 reads in 160.358 CPU sec, 5.110 real sec
[M::mem_process_seqs] Processed 675588 reads in 103.893 CPU sec, 3.282 real sec
[M::process] read 675980 sequences (100000298 bp) in 2.145 CPU sec, 2.210 real sec...
sorting block 29, #reads = 1080507 mem = 536880364 peak = 889270272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284048, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 330, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (350.44, 159.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676634 sequences (100000049 bp) in 2.239 CPU sec, 2.392 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284697, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 328, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (348.18, 158.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675484 sequences (100000142 bp) in 1.660 CPU sec, 1.662 real sec...
[M::mem_process_seqs] Processed 675830 reads in 163.013 CPU sec, 5.205 real sec
sorting block 30, #reads = 1081260 mem = 536879144 peak = 930213888
[M::mem_process_seqs] Processed 675980 reads in 105.935 CPU sec, 3.369 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285054, 1, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (243, 630, 6929)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 20301)
[M::mem_pestat] mean and std.dev: (2397.80, 2971.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 26987)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 324, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (343.23, 155.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (322, 601, 601)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1159)
[M::mem_pestat] mean and std.dev: (450.15, 171.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1438)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 31, #reads = 1080646 mem = 536880210 peak = 930213888
[M::process] read 675406 sequences (100000254 bp) in 2.359 CPU sec, 2.527 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284620, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 331, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (351.39, 160.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675658 sequences (100000200 bp) in 1.707 CPU sec, 1.711 real sec...
[M::mem_process_seqs] Processed 676634 reads in 165.718 CPU sec, 5.289 real sec
[M::mem_process_seqs] Processed 675484 reads in 103.183 CPU sec, 3.271 real sec
sorting block 32, #reads = 1079731 mem = 536884713 peak = 930213888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284705, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 330, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (350.49, 159.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675412 sequences (100000137 bp) in 2.337 CPU sec, 2.472 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284147, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 329, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (349.37, 158.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675090 sequences (100000001 bp) in 1.850 CPU sec, 1.858 real sec...
[M::mem_process_seqs] Processed 675406 reads in 160.639 CPU sec, 5.110 real sec
[M::mem_process_seqs] Processed 675658 reads in 102.996 CPU sec, 3.251 real sec
sorting block 33, #reads = 1081295 mem = 536880931 peak = 930213888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284980, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 330, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (350.30, 158.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675132 sequences (100000006 bp) in 2.726 CPU sec, 2.893 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284348, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 330, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (349.87, 157.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675412 reads in 156.836 CPU sec, 4.987 real sec
[M::process] read 674974 sequences (100000261 bp) in 1.609 CPU sec, 1.611 real sec...
[M::mem_process_seqs] Processed 675090 reads in 102.342 CPU sec, 3.234 real sec
sorting block 34, #reads = 1081241 mem = 536877911 peak = 930213888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284395, 8, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (376, 376, 1282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3094)
[M::mem_pestat] mean and std.dev: (481.27, 391.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4000)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 331, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (349.92, 157.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674744 sequences (100000228 bp) in 2.380 CPU sec, 2.539 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284642, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 332, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (350.88, 157.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675132 reads in 152.649 CPU sec, 4.862 real sec
[M::process] read 674926 sequences (100000005 bp) in 1.848 CPU sec, 1.862 real sec...
sorting block 35, #reads = 1081512 mem = 536879328 peak = 930213888
[M::mem_process_seqs] Processed 674974 reads in 95.862 CPU sec, 3.019 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284680, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 336, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (354.70, 159.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 36, #reads = 1080824 mem = 536880581 peak = 930213888
[M::process] read 675678 sequences (100000011 bp) in 2.563 CPU sec, 2.704 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284316, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 336, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (356.13, 161.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674744 reads in 141.044 CPU sec, 4.470 real sec
[M::process] read 676546 sequences (100000019 bp) in 1.649 CPU sec, 1.666 real sec...
[M::mem_process_seqs] Processed 674926 reads in 91.444 CPU sec, 2.880 real sec
sorting block 37, #reads = 1080750 mem = 536878798 peak = 930213888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284053, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 334, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (353.92, 162.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675890 sequences (100000100 bp) in 2.248 CPU sec, 2.379 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285322, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (348.91, 160.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675678 reads in 143.272 CPU sec, 4.555 real sec
[M::mem_process_seqs] Processed 676546 reads in 91.993 CPU sec, 2.891 real sec
[M::process] read 675742 sequences (100000085 bp) in 2.012 CPU sec, 2.056 real sec...
sorting block 38, #reads = 1080984 mem = 536878956 peak = 930213888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284975, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 335, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (353.34, 161.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675212 sequences (100000096 bp) in 2.380 CPU sec, 2.538 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284642, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 337, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (355.79, 162.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675890 reads in 140.360 CPU sec, 4.461 real sec
[M::process] read 674972 sequences (100000001 bp) in 1.572 CPU sec, 1.586 real sec...
[M::mem_process_seqs] Processed 675742 reads in 90.109 CPU sec, 2.834 real sec
sorting block 39, #reads = 1081344 mem = 536879568 peak = 930213888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284614, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 340, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (357.54, 161.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674858 sequences (100000026 bp) in 2.317 CPU sec, 2.450 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284970, 7, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 344, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (361.17, 162.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 334, 8138)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 24044)
[M::mem_pestat] mean and std.dev: (2488.18, 3468.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 31997)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675212 reads in 138.005 CPU sec, 4.399 real sec
[M::mem_process_seqs] Processed 674972 reads in 89.665 CPU sec, 2.821 real sec
sorting block 40, #reads = 1081178 mem = 536876558 peak = 930213888
[M::process] read 674512 sequences (100000149 bp) in 2.147 CPU sec, 2.235 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284914, 9, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 347, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (363.89, 164.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 41, #reads = 1081923 mem = 536878893 peak = 930213888
[M::process] read 674220 sequences (100000270 bp) in 2.302 CPU sec, 2.457 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284350, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 349, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (365.64, 164.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674858 reads in 143.148 CPU sec, 4.558 real sec
[M::mem_process_seqs] Processed 674512 reads in 92.698 CPU sec, 2.914 real sec
[M::process] read 674392 sequences (100000000 bp) in 2.008 CPU sec, 2.106 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 284939, 2, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (459, 753, 753)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1341)
[M::mem_pestat] mean and std.dev: (581.77, 217.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1635)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 353, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (369.84, 166.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 42, #reads = 1080853 mem = 536882214 peak = 930213888
[M::process] read 674286 sequences (100000126 bp) in 2.196 CPU sec, 2.335 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284805, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 354, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (371.33, 168.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674220 reads in 141.568 CPU sec, 4.512 real sec
[M::mem_process_seqs] Processed 674392 reads in 91.808 CPU sec, 2.896 real sec
[M::process] read 675338 sequences (100000030 bp) in 2.291 CPU sec, 2.394 real sec...
sorting block 43, #reads = 1081395 mem = 536881928 peak = 930213888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 285364, 1, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 239, 239)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 327)
[M::mem_pestat] mean and std.dev: (212.45, 39.23)
[M::mem_pestat] low and high boundaries for proper pairs: (56, 371)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 354, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (371.19, 167.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675440 sequences (100000183 bp) in 2.435 CPU sec, 2.606 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284601, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 342, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (361.25, 165.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674286 reads in 143.080 CPU sec, 4.560 real sec
[M::mem_process_seqs] Processed 675338 reads in 93.356 CPU sec, 2.946 real sec
sorting block 44, #reads = 1081871 mem = 536877229 peak = 930213888
[M::process] read 675220 sequences (100000301 bp) in 2.241 CPU sec, 2.372 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285372, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 338, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (357.59, 164.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675142 sequences (100000060 bp) in 2.031 CPU sec, 2.168 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284346, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 341, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (359.09, 163.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675440 reads in 141.933 CPU sec, 4.521 real sec
[M::mem_process_seqs] Processed 675220 reads in 90.518 CPU sec, 2.855 real sec
sorting block 45, #reads = 1080735 mem = 536877897 peak = 930213888
[M::process] read 675358 sequences (100000013 bp) in 2.090 CPU sec, 2.185 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284636, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 340, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (359.43, 164.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 46, #reads = 1081524 mem = 536879724 peak = 955805696
[M::process] read 675386 sequences (100000057 bp) in 2.096 CPU sec, 2.236 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285497, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 337, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (357.67, 163.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675142 reads in 142.945 CPU sec, 4.562 real sec
[M::mem_process_seqs] Processed 675358 reads in 91.757 CPU sec, 2.895 real sec
[M::process] read 675350 sequences (100000259 bp) in 1.910 CPU sec, 1.990 real sec...
sorting block 47, #reads = 1081844 mem = 536878362 peak = 955805696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285307, 9, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 337, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (357.29, 163.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675364 sequences (100000225 bp) in 2.118 CPU sec, 2.254 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284522, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 334, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (354.97, 162.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675386 reads in 139.107 CPU sec, 4.431 real sec
[M::mem_process_seqs] Processed 675350 reads in 89.233 CPU sec, 2.812 real sec
[M::process] read 675392 sequences (100000206 bp) in 1.601 CPU sec, 1.645 real sec...
sorting block 48, #reads = 1083551 mem = 536879869 peak = 955805696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284563, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 333, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (354.38, 162.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675836 sequences (100000053 bp) in 2.391 CPU sec, 2.522 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284337, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 332, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (352.81, 161.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675364 reads in 142.029 CPU sec, 4.518 real sec
[M::mem_process_seqs] Processed 675392 reads in 91.338 CPU sec, 2.870 real sec
sorting block 49, #reads = 1081968 mem = 536881552 peak = 955805696
[M::process] read 675456 sequences (100000237 bp) in 2.235 CPU sec, 2.318 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285161, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 328, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (348.67, 159.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675406 sequences (100000211 bp) in 2.206 CPU sec, 2.341 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284329, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 332, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (352.14, 160.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675836 reads in 141.448 CPU sec, 4.500 real sec
[M::mem_process_seqs] Processed 675456 reads in 90.011 CPU sec, 2.831 real sec
sorting block 50, #reads = 1082219 mem = 536877619 peak = 955805696
[M::process] read 675320 sequences (100000092 bp) in 2.090 CPU sec, 2.172 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284689, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 334, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (354.76, 162.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 51, #reads = 1079980 mem = 536878091 peak = 962785280
[M::process] read 675028 sequences (100000110 bp) in 2.327 CPU sec, 2.466 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285534, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 335, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (355.63, 163.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675406 reads in 142.059 CPU sec, 4.530 real sec
[M::mem_process_seqs] Processed 675320 reads in 91.954 CPU sec, 2.916 real sec
[M::process] read 675038 sequences (100000086 bp) in 2.119 CPU sec, 2.231 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284635, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 335, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (355.56, 162.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 52, #reads = 1080845 mem = 536880479 peak = 962785280
[M::process] read 675060 sequences (100000254 bp) in 2.120 CPU sec, 2.273 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284707, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 335, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (355.24, 162.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675028 reads in 143.129 CPU sec, 4.571 real sec
[M::mem_process_seqs] Processed 675038 reads in 91.907 CPU sec, 2.896 real sec
[M::process] read 675090 sequences (100000032 bp) in 1.716 CPU sec, 1.763 real sec...
sorting block 53, #reads = 1081937 mem = 536876538 peak = 962785280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284029, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 334, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (354.91, 162.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675004 sequences (100000115 bp) in 2.290 CPU sec, 2.440 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284330, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 336, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (356.03, 162.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675060 reads in 143.319 CPU sec, 4.563 real sec
[M::mem_process_seqs] Processed 675090 reads in 92.971 CPU sec, 2.923 real sec
[M::process] read 675186 sequences (100000068 bp) in 1.945 CPU sec, 2.015 real sec...
sorting block 54, #reads = 1081863 mem = 536883583 peak = 962785280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284958, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 336, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (357.22, 163.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674692 sequences (100000082 bp) in 2.194 CPU sec, 2.331 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284399, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 335, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (355.35, 163.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675004 reads in 139.799 CPU sec, 4.453 real sec
sorting block 55, #reads = 1080466 mem = 536880079 peak = 962785280
[M::mem_process_seqs] Processed 675186 reads in 90.468 CPU sec, 2.847 real sec
[M::process] read 675290 sequences (100000076 bp) in 1.748 CPU sec, 1.792 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284856, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 338, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (358.68, 163.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 56, #reads = 1081236 mem = 536877447 peak = 964734976
[M::process] read 676090 sequences (100000144 bp) in 2.178 CPU sec, 2.309 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 284590, 3, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (90, 180, 189)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387)
[M::mem_pestat] mean and std.dev: (160.23, 51.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 486)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 334, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (352.94, 160.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 674692 reads in 141.205 CPU sec, 4.492 real sec
[M::process] read 675544 sequences (100000255 bp) in 1.739 CPU sec, 1.766 real sec...
[M::mem_process_seqs] Processed 675290 reads in 93.848 CPU sec, 2.958 real sec
sorting block 57, #reads = 1080783 mem = 536878151 peak = 964734976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284587, 0, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 331, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (351.05, 160.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 313, 948)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2448)
[M::mem_pestat] mean and std.dev: (629.38, 756.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3656)
[M::mem_pestat] skip orientation RR
[M::process] read 675382 sequences (100000142 bp) in 2.271 CPU sec, 2.425 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284659, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 338, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (356.69, 163.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676090 reads in 151.500 CPU sec, 4.818 real sec
[M::process] read 674942 sequences (100000210 bp) in 1.802 CPU sec, 1.815 real sec...
[M::mem_process_seqs] Processed 675544 reads in 96.622 CPU sec, 3.046 real sec
sorting block 58, #reads = 1081916 mem = 536881028 peak = 964734976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284445, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 340, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (359.38, 164.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674838 sequences (100000074 bp) in 2.240 CPU sec, 2.386 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283928, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 344, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (362.84, 166.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674664 sequences (100000108 bp) in 1.530 CPU sec, 1.524 real sec...
[M::mem_process_seqs] Processed 675382 reads in 150.567 CPU sec, 4.789 real sec
[M::mem_process_seqs] Processed 674942 reads in 96.607 CPU sec, 3.050 real sec
sorting block 59, #reads = 1082439 mem = 536880435 peak = 964734976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284257, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 346, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (364.95, 167.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674572 sequences (100000215 bp) in 2.463 CPU sec, 2.656 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284073, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 348, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (366.52, 166.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674838 reads in 144.650 CPU sec, 4.599 real sec
sorting block 60, #reads = 1080969 mem = 536879738 peak = 964734976
[M::mem_process_seqs] Processed 674664 reads in 92.167 CPU sec, 2.898 real sec
[M::process] read 674098 sequences (100000179 bp) in 2.137 CPU sec, 2.222 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284701, 4, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 589, 9315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 27671)
[M::mem_pestat] mean and std.dev: (4388.09, 4500.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 36849)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 349, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (366.94, 167.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 61, #reads = 1081296 mem = 536879436 peak = 983126016
[M::process] read 674014 sequences (100000069 bp) in 2.234 CPU sec, 2.388 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 283938, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 353, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (370.08, 167.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674572 reads in 146.401 CPU sec, 4.662 real sec
[M::mem_process_seqs] Processed 674098 reads in 95.325 CPU sec, 2.998 real sec
[M::process] read 673736 sequences (100000206 bp) in 2.098 CPU sec, 2.182 real sec...
sorting block 62, #reads = 1082209 mem = 536879438 peak = 983126016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284110, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 354, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (370.59, 166.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674888 sequences (100000054 bp) in 2.257 CPU sec, 2.397 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283544, 3, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 357, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (373.79, 168.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (713, 713, 2469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5981)
[M::mem_pestat] mean and std.dev: (1287.00, 1064.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7737)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 674014 reads in 143.960 CPU sec, 4.585 real sec
[M::process] read 675330 sequences (100000066 bp) in 2.191 CPU sec, 2.291 real sec...
sorting block 63, #reads = 1081332 mem = 536878889 peak = 983126016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284275, 2, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (239, 430, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (346.50, 140.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 345, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (363.84, 166.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674840 sequences (100000287 bp) in 1.395 CPU sec, 1.403 real sec...
[M::mem_process_seqs] Processed 674888 reads in 84.553 CPU sec, 2.677 real sec
[M::mem_process_seqs] Processed 673736 reads in 204.466 CPU sec, 6.469 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284616, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 339, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (359.22, 165.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 377, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (334.10, 91.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RR
sorting block 64, #reads = 1082058 mem = 536879827 peak = 983126016
[M::process] read 674968 sequences (100000227 bp) in 2.300 CPU sec, 2.460 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284036, 5, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 161, 866)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2294)
[M::mem_pestat] mean and std.dev: (373.00, 321.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3008)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 344, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (363.27, 165.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675330 reads in 163.051 CPU sec, 5.160 real sec
[M::mem_process_seqs] Processed 674840 reads in 94.082 CPU sec, 2.967 real sec
[M::process] read 675368 sequences (100000058 bp) in 1.702 CPU sec, 1.751 real sec...
sorting block 65, #reads = 1081840 mem = 536876890 peak = 983126016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284135, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 341, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (360.18, 164.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675124 sequences (100000270 bp) in 2.534 CPU sec, 2.706 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 283951, 2, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (531, 531, 3420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9198)
[M::mem_pestat] mean and std.dev: (2500.18, 2917.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14170)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 337, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (357.41, 163.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 674968 reads in 149.982 CPU sec, 4.770 real sec
[M::mem_process_seqs] Processed 675368 reads in 95.421 CPU sec, 2.998 real sec
[M::process] read 675286 sequences (100000087 bp) in 2.016 CPU sec, 2.046 real sec...
sorting block 66, #reads = 1081845 mem = 536880478 peak = 984936448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284797, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 336, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (356.54, 162.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675374 sequences (100000208 bp) in 2.017 CPU sec, 2.119 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 284103, 0, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (223, 305, 1614)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4396)
[M::mem_pestat] mean and std.dev: (629.94, 623.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5787)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 334, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (354.69, 162.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675124 reads in 146.102 CPU sec, 4.648 real sec
[M::process] read 675394 sequences (100000189 bp) in 1.859 CPU sec, 1.868 real sec...
sorting block 67, #reads = 1081415 mem = 536880109 peak = 984936448
[M::mem_process_seqs] Processed 675286 reads in 94.056 CPU sec, 2.960 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284536, 6, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 333, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (352.91, 160.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (597, 597, 2986)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7764)
[M::mem_pestat] mean and std.dev: (1355.56, 1262.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10153)
[M::mem_pestat] skip orientation RR
sorting block 68, #reads = 1081919 mem = 536878189 peak = 984936448
[M::process] read 675034 sequences (100000015 bp) in 2.353 CPU sec, 2.492 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284328, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 333, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (353.16, 160.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675050 sequences (100000234 bp) in 1.440 CPU sec, 1.438 real sec...
[M::mem_process_seqs] Processed 675374 reads in 146.435 CPU sec, 4.648 real sec
[M::mem_process_seqs] Processed 675394 reads in 93.740 CPU sec, 2.957 real sec
sorting block 69, #reads = 1081135 mem = 536876155 peak = 984936448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284408, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 335, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (354.53, 160.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675088 sequences (100000246 bp) in 2.205 CPU sec, 2.334 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284480, 6, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (216, 361, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (287.40, 126.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 334, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (353.94, 160.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675034 reads in 146.004 CPU sec, 4.639 real sec
[M::process] read 675298 sequences (100000143 bp) in 1.720 CPU sec, 1.728 real sec...
[M::mem_process_seqs] Processed 675050 reads in 93.627 CPU sec, 2.953 real sec
sorting block 70, #reads = 1081222 mem = 536878437 peak = 984936448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284299, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 335, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (355.45, 162.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675266 sequences (100000238 bp) in 2.318 CPU sec, 2.470 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284051, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 335, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (355.76, 163.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675088 reads in 144.286 CPU sec, 4.590 real sec
[M::mem_process_seqs] Processed 675298 reads in 92.941 CPU sec, 2.923 real sec
[M::process] read 675206 sequences (100000133 bp) in 1.918 CPU sec, 1.958 real sec...
sorting block 71, #reads = 1080662 mem = 536877989 peak = 984936448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284844, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 334, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (355.88, 163.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675148 sequences (100000210 bp) in 2.058 CPU sec, 2.205 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284673, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 334, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (354.45, 161.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674886 sequences (100000297 bp) in 1.430 CPU sec, 1.437 real sec...
[M::mem_process_seqs] Processed 675266 reads in 146.292 CPU sec, 4.659 real sec
[M::mem_process_seqs] Processed 675206 reads in 93.136 CPU sec, 2.943 real sec
sorting block 72, #reads = 1080654 mem = 536878766 peak = 984936448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284290, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 335, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (354.54, 160.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 73, #reads = 1081890 mem = 536879841 peak = 985608192
[M::process] read 674722 sequences (100000145 bp) in 2.725 CPU sec, 2.884 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284706, 3, 18)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 336, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (356.00, 161.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (368, 510, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (84, 794)
[M::mem_pestat] mean and std.dev: (396.88, 138.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675148 reads in 141.750 CPU sec, 4.502 real sec
[M::mem_process_seqs] Processed 674886 reads in 90.916 CPU sec, 2.857 real sec
[M::process] read 674794 sequences (100000019 bp) in 2.225 CPU sec, 2.332 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284886, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 339, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (358.92, 162.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 74, #reads = 1080258 mem = 536879701 peak = 985608192
[M::process] read 674742 sequences (100000105 bp) in 2.146 CPU sec, 2.290 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284773, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 338, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (357.82, 161.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674722 reads in 144.495 CPU sec, 4.601 real sec
[M::mem_process_seqs] Processed 674794 reads in 93.388 CPU sec, 2.935 real sec
[M::process] read 674310 sequences (100000078 bp) in 2.164 CPU sec, 2.246 real sec...
sorting block 75, #reads = 1081587 mem = 536881581 peak = 985608192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285293, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 340, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (359.67, 163.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679532 sequences (100000004 bp) in 2.441 CPU sec, 2.592 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 283378, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 343, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (361.70, 163.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674742 reads in 142.699 CPU sec, 4.545 real sec
[M::mem_process_seqs] Processed 674310 reads in 92.422 CPU sec, 2.908 real sec
sorting block 76, #reads = 1080545 mem = 536880114 peak = 985608192
[M::process] read 678580 sequences (100000293 bp) in 2.379 CPU sec, 2.512 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286142, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 308, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (333.75, 159.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678428 sequences (100000156 bp) in 2.347 CPU sec, 2.462 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286034, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 314, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (339.68, 161.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679532 reads in 156.630 CPU sec, 4.976 real sec
sorting block 77, #reads = 1079842 mem = 536878114 peak = 985608192
[M::process] read 678546 sequences (100000155 bp) in 2.089 CPU sec, 2.151 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285733, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 317, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (340.73, 160.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677846 sequences (100000097 bp) in 1.752 CPU sec, 1.760 real sec...
[M::mem_process_seqs] Processed 678428 reads in 91.008 CPU sec, 2.884 real sec
sorting block 78, #reads = 1080203 mem = 536877071 peak = 991485952
[M::mem_process_seqs] Processed 678580 reads in 219.398 CPU sec, 6.942 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286354, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 317, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (338.75, 158.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677076 sequences (100000208 bp) in 2.348 CPU sec, 2.481 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285534, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 323, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (343.48, 159.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678546 reads in 173.030 CPU sec, 5.472 real sec
[M::process] read 676646 sequences (100000263 bp) in 1.581 CPU sec, 1.584 real sec...
[M::mem_process_seqs] Processed 677846 reads in 101.409 CPU sec, 3.198 real sec
sorting block 79, #reads = 1079793 mem = 536888616 peak = 991485952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285876, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 330, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (348.15, 159.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676724 sequences (100000101 bp) in 2.152 CPU sec, 2.292 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285510, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 333, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (350.78, 159.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677342 sequences (100000224 bp) in 1.729 CPU sec, 1.736 real sec...
[M::mem_process_seqs] Processed 677076 reads in 156.650 CPU sec, 4.974 real sec
sorting block 80, #reads = 1078103 mem = 536880857 peak = 991485952
[M::mem_process_seqs] Processed 676646 reads in 97.764 CPU sec, 3.090 real sec
sorting block 81, #reads = 1078633 mem = 536878207 peak = 1031364608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285334, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 333, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (351.14, 160.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678248 sequences (100000280 bp) in 2.587 CPU sec, 2.709 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286506, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 328, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (346.17, 158.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676724 reads in 157.064 CPU sec, 4.980 real sec
[M::process] read 678584 sequences (100000144 bp) in 2.033 CPU sec, 2.057 real sec...
[M::mem_process_seqs] Processed 677342 reads in 100.752 CPU sec, 3.165 real sec
sorting block 82, #reads = 1080091 mem = 536876520 peak = 1031364608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285968, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 317, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (338.00, 156.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678358 sequences (100000194 bp) in 2.510 CPU sec, 2.657 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286227, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 315, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (336.11, 155.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678248 reads in 160.344 CPU sec, 5.087 real sec
[M::process] read 678524 sequences (100000139 bp) in 1.971 CPU sec, 1.982 real sec...
[M::mem_process_seqs] Processed 678584 reads in 103.330 CPU sec, 3.245 real sec
sorting block 83, #reads = 1078931 mem = 536879588 peak = 1031364608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285922, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 317, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (338.52, 156.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678086 sequences (100000053 bp) in 2.514 CPU sec, 2.627 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286499, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 316, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (337.95, 156.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677112 sequences (100000181 bp) in 1.740 CPU sec, 1.740 real sec...
[M::mem_process_seqs] Processed 678358 reads in 165.264 CPU sec, 5.246 real sec
sorting block 84, #reads = 1077623 mem = 536879235 peak = 1031364608
[M::mem_process_seqs] Processed 678524 reads in 105.811 CPU sec, 3.336 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285955, 9, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 320, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (340.36, 157.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 85, #reads = 1076893 mem = 536878880 peak = 1031364608
[M::process] read 677168 sequences (100000246 bp) in 2.243 CPU sec, 2.408 real sec...
[M::process] read 677816 sequences (100000073 bp) in 1.570 CPU sec, 1.566 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285935, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 327, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (346.55, 159.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678086 reads in 164.606 CPU sec, 5.234 real sec
[M::mem_process_seqs] Processed 677112 reads in 103.824 CPU sec, 3.293 real sec
sorting block 86, #reads = 1077464 mem = 536879961 peak = 1031364608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285293, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 325, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (344.06, 157.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679014 sequences (100000247 bp) in 2.290 CPU sec, 2.414 real sec...
[M::process] read 677500 sequences (100000275 bp) in 1.804 CPU sec, 1.803 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286042, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 320, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (339.79, 155.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677168 reads in 164.916 CPU sec, 5.235 real sec
[M::mem_process_seqs] Processed 677816 reads in 108.196 CPU sec, 3.425 real sec
sorting block 87, #reads = 1078010 mem = 536877397 peak = 1031364608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285943, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 313, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (332.49, 152.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676838 sequences (100000171 bp) in 2.409 CPU sec, 2.538 real sec...
[M::process] read 676554 sequences (100000130 bp) in 1.700 CPU sec, 1.699 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285168, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 318, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (337.81, 152.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679014 reads in 181.026 CPU sec, 5.750 real sec
[M::mem_process_seqs] Processed 677500 reads in 111.916 CPU sec, 3.549 real sec
sorting block 88, #reads = 1071499 mem = 536881609 peak = 1031364608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 285737, 9, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (338, 421, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (172, 587)
[M::mem_pestat] mean and std.dev: (389.50, 51.05)
[M::mem_pestat] low and high boundaries for proper pairs: (89, 670)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 324, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (344.34, 156.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 676760 sequences (100000142 bp) in 2.329 CPU sec, 2.444 real sec...
[M::process] read 676540 sequences (100000140 bp) in 1.610 CPU sec, 1.606 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285428, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 326, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (345.28, 156.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676838 reads in 167.180 CPU sec, 5.309 real sec
sorting block 89, #reads = 1074103 mem = 536888653 peak = 1031364608
[M::mem_process_seqs] Processed 676554 reads in 105.980 CPU sec, 3.357 real sec
sorting block 90, #reads = 1075086 mem = 536883628 peak = 1031364608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285208, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 326, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (345.73, 157.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1579, 1579, 2364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 3934)
[M::mem_pestat] mean and std.dev: (1691.70, 615.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4719)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676860 sequences (100000129 bp) in 2.212 CPU sec, 2.343 real sec...
[M::process] read 676628 sequences (100000256 bp) in 1.640 CPU sec, 1.642 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285244, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 330, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (349.76, 159.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676760 reads in 165.644 CPU sec, 5.265 real sec
[M::mem_process_seqs] Processed 676540 reads in 105.820 CPU sec, 3.356 real sec
sorting block 91, #reads = 1075125 mem = 536878359 peak = 1031364608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285721, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 323, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (344.74, 157.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677560 sequences (100000108 bp) in 2.252 CPU sec, 2.364 real sec...
[M::process] read 681978 sequences (100000049 bp) in 1.780 CPU sec, 1.775 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285565, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 325, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (346.66, 159.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676860 reads in 169.630 CPU sec, 5.383 real sec
[M::mem_process_seqs] Processed 676628 reads in 108.177 CPU sec, 3.430 real sec
sorting block 92, #reads = 1073919 mem = 536877574 peak = 1031364608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285842, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 321, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (344.08, 161.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679474 sequences (100000121 bp) in 2.483 CPU sec, 2.600 real sec...
[M::process] read 679096 sequences (100000023 bp) in 1.570 CPU sec, 1.569 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286953, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 290, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (315.39, 148.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1070)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677560 reads in 176.165 CPU sec, 5.606 real sec
sorting block 93, #reads = 1074669 mem = 536882476 peak = 1031364608
[M::mem_process_seqs] Processed 681978 reads in 115.029 CPU sec, 3.649 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286687, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 304, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (326.98, 151.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679012 sequences (100000174 bp) in 2.448 CPU sec, 2.557 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286207, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 310, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (332.60, 154.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677808 sequences (100000150 bp) in 1.890 CPU sec, 1.891 real sec...
[M::mem_process_seqs] Processed 679474 reads in 166.430 CPU sec, 5.277 real sec
sorting block 94, #reads = 1071038 mem = 536878405 peak = 1031364608
[M::mem_process_seqs] Processed 679096 reads in 104.299 CPU sec, 3.296 real sec
sorting block 95, #reads = 1074843 mem = 536880352 peak = 1031872512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286463, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 311, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (332.91, 154.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677640 sequences (100000176 bp) in 2.387 CPU sec, 2.524 real sec...
[M::process] read 677034 sequences (100000007 bp) in 1.590 CPU sec, 1.593 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286458, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 320, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (338.34, 153.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679012 reads in 163.004 CPU sec, 5.177 real sec
[M::mem_process_seqs] Processed 677808 reads in 103.269 CPU sec, 3.270 real sec
sorting block 96, #reads = 1074172 mem = 536879406 peak = 1031872512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286276, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 323, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (341.12, 155.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677806 sequences (100000049 bp) in 2.479 CPU sec, 2.582 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286207, 2, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 326, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (344.84, 156.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 197, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1112)
[M::mem_pestat] mean and std.dev: (252.45, 117.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1417)
[M::mem_pestat] skip orientation RR
[M::process] read 678154 sequences (100000048 bp) in 1.880 CPU sec, 1.878 real sec...
[M::mem_process_seqs] Processed 677640 reads in 161.200 CPU sec, 5.106 real sec
[M::mem_process_seqs] Processed 677034 reads in 102.669 CPU sec, 3.231 real sec
sorting block 97, #reads = 1075434 mem = 536879109 peak = 1031872512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285962, 8, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 319, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (339.72, 156.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (598, 598, 2109)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5131)
[M::mem_pestat] mean and std.dev: (1093.45, 778.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6642)
[M::mem_pestat] skip orientation RR
[M::process] read 678052 sequences (100000047 bp) in 2.321 CPU sec, 2.435 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286480, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 315, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (336.86, 156.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677820 sequences (100000269 bp) in 1.450 CPU sec, 1.451 real sec...
[M::mem_process_seqs] Processed 677806 reads in 154.109 CPU sec, 4.903 real sec
sorting block 98, #reads = 1076881 mem = 536878959 peak = 1031872512
[M::mem_process_seqs] Processed 678154 reads in 98.289 CPU sec, 3.107 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285701, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 317, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (337.58, 155.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 285, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (251.00, 65.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RR
[M::process] read 677842 sequences (100000247 bp) in 2.570 CPU sec, 2.673 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285822, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 317, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (339.36, 157.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678052 reads in 151.341 CPU sec, 4.797 real sec
sorting block 99, #reads = 1078198 mem = 536881058 peak = 1031872512
[M::process] read 677996 sequences (100000269 bp) in 2.005 CPU sec, 2.021 real sec...
[M::mem_process_seqs] Processed 677820 reads in 96.834 CPU sec, 3.040 real sec
sorting block 100, #reads = 1078372 mem = 536877605 peak = 1031872512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286252, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 321, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (341.76, 158.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677378 sequences (100000075 bp) in 2.444 CPU sec, 2.570 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286383, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 319, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (339.81, 156.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677842 reads in 157.346 CPU sec, 4.991 real sec
[M::process] read 677112 sequences (100000118 bp) in 1.877 CPU sec, 1.876 real sec...
[M::mem_process_seqs] Processed 677996 reads in 101.446 CPU sec, 3.197 real sec
sorting block 101, #reads = 1078347 mem = 536881060 peak = 1031872512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285820, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 322, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (342.81, 157.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679208 sequences (100000122 bp) in 2.289 CPU sec, 2.420 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285615, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 323, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (344.02, 158.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676554 sequences (100000265 bp) in 1.790 CPU sec, 1.788 real sec...
[M::mem_process_seqs] Processed 677378 reads in 161.307 CPU sec, 5.118 real sec
[M::mem_process_seqs] Processed 677112 reads in 102.547 CPU sec, 3.246 real sec
sorting block 102, #reads = 1076220 mem = 536879238 peak = 1031872512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286576, 11, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 308, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (330.32, 153.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (362, 507, 2022)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5342)
[M::mem_pestat] mean and std.dev: (823.91, 802.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7002)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676254 sequences (100000075 bp) in 2.390 CPU sec, 2.525 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285234, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 331, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (353.13, 164.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676324 sequences (100000213 bp) in 1.840 CPU sec, 1.845 real sec...
[M::mem_process_seqs] Processed 679208 reads in 169.247 CPU sec, 5.371 real sec
sorting block 103, #reads = 1073203 mem = 536879770 peak = 1031872512
[M::mem_process_seqs] Processed 676554 reads in 103.962 CPU sec, 3.286 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284826, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 332, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (353.66, 164.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 104, #reads = 1076294 mem = 536879973 peak = 1031872512
[M::process] read 676084 sequences (100000190 bp) in 2.575 CPU sec, 2.680 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284950, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 331, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (353.88, 165.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676254 reads in 156.300 CPU sec, 4.951 real sec
[M::process] read 676076 sequences (100000127 bp) in 2.011 CPU sec, 2.028 real sec...
[M::mem_process_seqs] Processed 676324 reads in 101.156 CPU sec, 3.179 real sec
sorting block 105, #reads = 1077823 mem = 536877671 peak = 1031872512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285360, 0, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 198, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (244.50, 113.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 335, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (357.74, 166.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675726 sequences (100000251 bp) in 2.544 CPU sec, 2.702 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285596, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 338, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (360.32, 168.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676084 reads in 157.384 CPU sec, 5.007 real sec
[M::mem_process_seqs] Processed 676076 reads in 101.713 CPU sec, 3.199 real sec
[M::process] read 675740 sequences (100000012 bp) in 2.092 CPU sec, 2.116 real sec...
sorting block 106, #reads = 1078073 mem = 536879359 peak = 1031872512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284508, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 340, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (362.01, 169.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675588 sequences (100000198 bp) in 2.467 CPU sec, 2.590 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284387, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 338, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (361.70, 170.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675726 reads in 153.697 CPU sec, 4.890 real sec
[M::mem_process_seqs] Processed 675740 reads in 98.703 CPU sec, 3.106 real sec
[M::process] read 676220 sequences (100000156 bp) in 2.355 CPU sec, 2.403 real sec...
sorting block 107, #reads = 1079120 mem = 536877658 peak = 1031872512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285038, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 338, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (361.88, 171.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678914 sequences (100000187 bp) in 2.401 CPU sec, 2.523 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285338, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 333, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (357.23, 169.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677696 sequences (100000188 bp) in 1.800 CPU sec, 1.802 real sec...
[M::mem_process_seqs] Processed 675588 reads in 171.231 CPU sec, 5.434 real sec
sorting block 108, #reads = 1078238 mem = 536880210 peak = 1031872512
[M::mem_process_seqs] Processed 676220 reads in 117.563 CPU sec, 3.702 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286476, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 109, #reads = 1071732 mem = 536878862 peak = 1031872512
[M::mem_pestat] (25, 50, 75) percentile: (209, 311, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (339.53, 164.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677342 sequences (100000262 bp) in 2.515 CPU sec, 2.647 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285734, 12, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 325, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (348.98, 166.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (351, 351, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (5, 870)
[M::mem_pestat] mean and std.dev: (368.00, 124.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676806 sequences (100000113 bp) in 1.560 CPU sec, 1.562 real sec...
[M::mem_process_seqs] Processed 678914 reads in 155.456 CPU sec, 4.925 real sec
[M::mem_process_seqs] Processed 677696 reads in 96.254 CPU sec, 3.039 real sec
sorting block 110, #reads = 1078637 mem = 536884211 peak = 1031872512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286073, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 329, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (352.28, 167.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676588 sequences (100000111 bp) in 2.276 CPU sec, 2.397 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285128, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 329, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (352.55, 166.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675798 sequences (100000065 bp) in 1.489 CPU sec, 1.489 real sec...
[M::mem_process_seqs] Processed 677342 reads in 147.922 CPU sec, 4.697 real sec
[M::mem_process_seqs] Processed 676806 reads in 95.491 CPU sec, 3.012 real sec
sorting block 111, #reads = 1081447 mem = 536878438 peak = 1031872512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285359, 1, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 331, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (352.52, 164.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (139, 160, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1198)
[M::mem_pestat] mean and std.dev: (246.36, 197.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1551)
[M::mem_pestat] skip orientation RR
[M::process] read 675906 sequences (100000211 bp) in 2.483 CPU sec, 2.618 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284573, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 338, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (357.11, 164.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676588 reads in 147.566 CPU sec, 4.687 real sec
[M::mem_process_seqs] Processed 675798 reads in 93.556 CPU sec, 2.939 real sec
[M::process] read 675852 sequences (100000163 bp) in 2.065 CPU sec, 2.109 real sec...
sorting block 112, #reads = 1080493 mem = 536879021 peak = 1031872512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285772, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 338, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (357.04, 164.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675828 sequences (100000064 bp) in 2.319 CPU sec, 2.465 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285979, 3, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 342, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (360.72, 167.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 197, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (177, 227)
[M::mem_pestat] mean and std.dev: (198.55, 6.34)
[M::mem_pestat] low and high boundaries for proper pairs: (167, 237)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675906 reads in 139.349 CPU sec, 4.429 real sec
[M::mem_process_seqs] Processed 675852 reads in 87.065 CPU sec, 2.734 real sec
sorting block 113, #reads = 1080960 mem = 536879862 peak = 1031872512
[M::process] read 677048 sequences (100000184 bp) in 2.194 CPU sec, 2.261 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285475, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 341, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (360.52, 168.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 114, #reads = 1081285 mem = 536880647 peak = 1031872512
[M::process] read 677906 sequences (100000090 bp) in 2.080 CPU sec, 2.208 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285607, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 328, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (349.70, 164.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675828 reads in 137.482 CPU sec, 4.378 real sec
[M::mem_process_seqs] Processed 677048 reads in 89.530 CPU sec, 2.823 real sec
[M::process] read 676868 sequences (100000148 bp) in 1.905 CPU sec, 1.970 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285812, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 317, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (341.99, 162.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 115, #reads = 1080974 mem = 536881675 peak = 1031872512
[M::process] read 676702 sequences (100000033 bp) in 2.352 CPU sec, 2.467 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285890, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 329, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (350.75, 164.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677906 reads in 147.670 CPU sec, 4.706 real sec
[M::mem_process_seqs] Processed 676868 reads in 94.953 CPU sec, 2.990 real sec
[M::process] read 676518 sequences (100000105 bp) in 2.077 CPU sec, 2.154 real sec...
sorting block 116, #reads = 1080916 mem = 536879288 peak = 1031872512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285936, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 332, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (354.12, 166.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676110 sequences (100000223 bp) in 2.317 CPU sec, 2.448 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285405, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 333, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (355.13, 165.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676702 reads in 148.743 CPU sec, 4.731 real sec
[M::process] read 675658 sequences (100000159 bp) in 1.596 CPU sec, 1.631 real sec...
[M::mem_process_seqs] Processed 676518 reads in 97.520 CPU sec, 3.067 real sec
sorting block 117, #reads = 1080356 mem = 536878729 peak = 1031872512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285557, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 337, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (358.92, 168.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675644 sequences (100000259 bp) in 2.342 CPU sec, 2.482 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284878, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 339, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (361.13, 169.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675954 sequences (100000130 bp) in 1.410 CPU sec, 1.408 real sec...
[M::mem_process_seqs] Processed 676110 reads in 147.996 CPU sec, 4.704 real sec
sorting block 118, #reads = 1077828 mem = 536877024 peak = 1031872512
[M::mem_process_seqs] Processed 675658 reads in 92.460 CPU sec, 2.916 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285137, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 339, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (361.90, 170.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 119, #reads = 1079504 mem = 536878347 peak = 1031872512
[M::process] read 676796 sequences (100000094 bp) in 2.328 CPU sec, 2.442 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285274, 4, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 333, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (357.35, 168.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (461, 1240, 2924)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7850)
[M::mem_pestat] mean and std.dev: (1859.08, 1921.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10313)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675644 reads in 141.356 CPU sec, 4.489 real sec
[M::process] read 676192 sequences (100000083 bp) in 1.754 CPU sec, 1.780 real sec...
[M::mem_process_seqs] Processed 675954 reads in 89.102 CPU sec, 2.798 real sec
sorting block 120, #reads = 1080746 mem = 536879231 peak = 1031872512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286191, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 326, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (350.20, 164.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676238 sequences (100000200 bp) in 2.163 CPU sec, 2.309 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285720, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 332, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (356.75, 169.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676368 sequences (100000211 bp) in 1.520 CPU sec, 1.515 real sec...
[M::mem_process_seqs] Processed 676796 reads in 143.215 CPU sec, 4.552 real sec
[M::mem_process_seqs] Processed 676192 reads in 90.122 CPU sec, 2.838 real sec
sorting block 121, #reads = 1076758 mem = 536881859 peak = 1031872512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285179, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 332, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (357.18, 169.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676318 sequences (100000292 bp) in 2.337 CPU sec, 2.474 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285109, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 333, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (357.63, 169.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676238 reads in 139.217 CPU sec, 4.422 real sec
[M::process] read 676360 sequences (100000147 bp) in 1.699 CPU sec, 1.718 real sec...
[M::mem_process_seqs] Processed 676368 reads in 87.424 CPU sec, 2.751 real sec
sorting block 122, #reads = 1079585 mem = 536879449 peak = 1031872512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285262, 8, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (218, 335, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872)
[M::mem_pestat] mean and std.dev: (298.11, 127.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1090)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 330, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (356.80, 170.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 676336 sequences (100000151 bp) in 2.083 CPU sec, 2.215 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285346, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 208, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (249.45, 88.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 853)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 331, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (358.32, 172.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 676754 sequences (100000089 bp) in 1.430 CPU sec, 1.425 real sec...
[M::mem_process_seqs] Processed 676318 reads in 141.490 CPU sec, 4.504 real sec
sorting block 123, #reads = 1080269 mem = 536878429 peak = 1031872512
[M::mem_process_seqs] Processed 676360 reads in 89.591 CPU sec, 2.825 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285034, 4, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 331, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (357.95, 172.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (413, 477, 619)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (468.80, 76.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RR
sorting block 124, #reads = 1078453 mem = 536882843 peak = 1042006016
[M::process] read 676470 sequences (100000278 bp) in 2.270 CPU sec, 2.380 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285851, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 325, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (352.81, 170.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676336 reads in 141.186 CPU sec, 4.485 real sec
[M::process] read 678852 sequences (100000221 bp) in 1.603 CPU sec, 1.615 real sec...
[M::mem_process_seqs] Processed 676754 reads in 90.186 CPU sec, 2.842 real sec
sorting block 125, #reads = 1078033 mem = 536877934 peak = 1042006016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285380, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 327, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (354.21, 169.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679440 sequences (100000020 bp) in 2.211 CPU sec, 2.346 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286222, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 311, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (339.33, 164.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678298 sequences (100000196 bp) in 1.580 CPU sec, 1.578 real sec...
[M::mem_process_seqs] Processed 676470 reads in 150.811 CPU sec, 4.789 real sec
[M::mem_process_seqs] Processed 678852 reads in 95.701 CPU sec, 3.028 real sec
sorting block 126, #reads = 1075022 mem = 536878603 peak = 1042006016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286658, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 308, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (335.64, 162.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677596 sequences (100000032 bp) in 2.326 CPU sec, 2.434 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286282, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 320, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (344.65, 164.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679440 reads in 141.177 CPU sec, 4.478 real sec
[M::process] read 676760 sequences (100000054 bp) in 1.570 CPU sec, 1.578 real sec...
[M::mem_process_seqs] Processed 678298 reads in 87.927 CPU sec, 2.774 real sec
sorting block 127, #reads = 1072679 mem = 536884467 peak = 1042006016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285970, 17, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 326, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (349.42, 165.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1476, 1476, 1821)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (786, 2511)
[M::mem_pestat] mean and std.dev: (1574.57, 155.86)
[M::mem_pestat] low and high boundaries for proper pairs: (441, 2856)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676692 sequences (100000243 bp) in 2.289 CPU sec, 2.427 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285676, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 331, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (351.52, 163.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677596 reads in 136.931 CPU sec, 4.351 real sec
sorting block 128, #reads = 1077258 mem = 536881702 peak = 1042006016
[M::process] read 676096 sequences (100000117 bp) in 1.696 CPU sec, 1.718 real sec...
[M::mem_process_seqs] Processed 676760 reads in 86.248 CPU sec, 2.714 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 286033, 1, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (124, 531, 531)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1345)
[M::mem_pestat] mean and std.dev: (331.17, 201.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1752)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 331, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (352.65, 164.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 129, #reads = 1077044 mem = 536878318 peak = 1042006016
[M::process] read 675844 sequences (100000156 bp) in 2.216 CPU sec, 2.342 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285275, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 339, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (357.11, 163.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676692 reads in 138.627 CPU sec, 4.421 real sec
[M::mem_process_seqs] Processed 676096 reads in 89.275 CPU sec, 2.812 real sec
[M::process] read 675772 sequences (100000105 bp) in 1.904 CPU sec, 1.949 real sec...
sorting block 130, #reads = 1076248 mem = 536878398 peak = 1042006016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284543, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 342, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (359.87, 164.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676564 sequences (100000055 bp) in 2.267 CPU sec, 2.421 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285355, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 344, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (362.06, 166.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2172, 2172, 2172)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2172, 2172)
[M::mem_pestat] mean and std.dev: (2172.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (2172, 2172)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675844 reads in 136.252 CPU sec, 4.341 real sec
[M::mem_process_seqs] Processed 675772 reads in 85.341 CPU sec, 2.687 real sec
[M::process] read 676256 sequences (100000284 bp) in 1.915 CPU sec, 1.988 real sec...
sorting block 131, #reads = 1076772 mem = 536881585 peak = 1042006016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285512, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 331, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (352.25, 163.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675732 sequences (100000174 bp) in 2.226 CPU sec, 2.368 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285140, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 332, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (352.12, 161.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676564 reads in 136.479 CPU sec, 4.356 real sec
[M::mem_process_seqs] Processed 676256 reads in 86.181 CPU sec, 2.718 real sec
sorting block 132, #reads = 1077234 mem = 536877709 peak = 1042006016
[M::process] read 675748 sequences (100000210 bp) in 2.137 CPU sec, 2.235 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284712, 1, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (619, 619, 868)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 1366)
[M::mem_pestat] mean and std.dev: (679.00, 148.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1615)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 337, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (355.85, 162.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 133, #reads = 1078703 mem = 536879381 peak = 1042006016
[M::process] read 675732 sequences (100000052 bp) in 2.100 CPU sec, 2.220 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 285301, 5, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 198, 774)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2012)
[M::mem_pestat] mean and std.dev: (447.83, 478.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2631)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 335, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (355.30, 162.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (346, 346, 1846)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4846)
[M::mem_pestat] mean and std.dev: (917.10, 760.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6346)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675732 reads in 138.244 CPU sec, 4.414 real sec
[M::mem_process_seqs] Processed 675748 reads in 87.729 CPU sec, 2.769 real sec
[M::process] read 675894 sequences (100000243 bp) in 2.010 CPU sec, 2.095 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284731, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 335, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (354.35, 161.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (397, 403, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (385, 415)
[M::mem_pestat] mean and std.dev: (401.00, 2.83)
[M::mem_pestat] low and high boundaries for proper pairs: (379, 421)
[M::mem_pestat] skip orientation RR
sorting block 134, #reads = 1078962 mem = 536883019 peak = 1042006016
[M::process] read 675502 sequences (100000210 bp) in 2.097 CPU sec, 2.224 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285338, 10, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 329, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (348.11, 156.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1909, 1909, 2152)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1423, 2638)
[M::mem_pestat] mean and std.dev: (2059.56, 229.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1142, 2977)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675732 reads in 142.795 CPU sec, 4.553 real sec
[M::mem_process_seqs] Processed 675894 reads in 91.483 CPU sec, 2.884 real sec
[M::process] read 675570 sequences (100000223 bp) in 1.788 CPU sec, 1.838 real sec...
sorting block 135, #reads = 1078488 mem = 536878598 peak = 1042006016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284716, 11, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 331, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (350.40, 157.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (597, 1839, 2040)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4926)
[M::mem_pestat] mean and std.dev: (1374.18, 1021.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6369)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675496 sequences (100000050 bp) in 2.230 CPU sec, 2.386 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284787, 1, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 330, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (350.91, 159.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 361, 904)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2174)
[M::mem_pestat] mean and std.dev: (498.00, 344.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2809)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675502 reads in 139.455 CPU sec, 4.445 real sec
[M::process] read 675618 sequences (100000205 bp) in 1.617 CPU sec, 1.645 real sec...
sorting block 136, #reads = 1076116 mem = 536883967 peak = 1042006016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284641, 0, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (393, 393, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (159, 744)
[M::mem_pestat] mean and std.dev: (419.92, 112.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 328, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (349.52, 159.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675382 sequences (100000241 bp) in 1.568 CPU sec, 1.578 real sec...
[M::mem_process_seqs] Processed 675496 reads in 75.486 CPU sec, 2.390 real sec
[M::mem_process_seqs] Processed 675570 reads in 188.769 CPU sec, 5.974 real sec
sorting block 137, #reads = 1079190 mem = 536880250 peak = 1042006016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284506, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 329, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (350.23, 159.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675498 sequences (100000080 bp) in 2.190 CPU sec, 2.329 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284500, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 327, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (347.28, 157.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 290, 547)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1107)
[M::mem_pestat] mean and std.dev: (401.82, 253.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1417)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675618 reads in 148.564 CPU sec, 4.704 real sec
[M::mem_process_seqs] Processed 675382 reads in 86.085 CPU sec, 2.716 real sec
[M::process] read 675854 sequences (100000220 bp) in 1.793 CPU sec, 1.854 real sec...
sorting block 138, #reads = 1077908 mem = 536878407 peak = 1042006016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 285123, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (256, 320, 1927)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5269)
[M::mem_pestat] mean and std.dev: (938.75, 836.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6940)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 327, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (349.23, 159.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675560 sequences (100000254 bp) in 2.162 CPU sec, 2.290 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284327, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 325, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (347.43, 159.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675498 reads in 135.647 CPU sec, 4.316 real sec
[M::mem_process_seqs] Processed 675854 reads in 85.713 CPU sec, 2.699 real sec
[M::process] read 675502 sequences (100000108 bp) in 1.733 CPU sec, 1.773 real sec...
sorting block 139, #reads = 1078870 mem = 536879235 peak = 1042006016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284761, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 327, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (349.52, 160.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675408 sequences (100000053 bp) in 2.289 CPU sec, 2.427 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284721, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 328, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (349.38, 159.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675560 reads in 135.874 CPU sec, 4.337 real sec
sorting block 140, #reads = 1079414 mem = 536878356 peak = 1042006016
[M::mem_process_seqs] Processed 675502 reads in 85.225 CPU sec, 2.690 real sec
[M::process] read 675382 sequences (100000094 bp) in 2.024 CPU sec, 2.112 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285396, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 329, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (350.80, 160.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 141, #reads = 1080656 mem = 536878843 peak = 1042006016
[M::process] read 675474 sequences (100000262 bp) in 2.092 CPU sec, 2.226 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284454, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 329, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (351.65, 161.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675408 reads in 134.499 CPU sec, 4.291 real sec
[M::mem_process_seqs] Processed 675382 reads in 85.488 CPU sec, 2.693 real sec
[M::process] read 675324 sequences (100000013 bp) in 1.914 CPU sec, 1.990 real sec...
sorting block 142, #reads = 1080201 mem = 536877577 peak = 1042006016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284889, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 330, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (352.68, 163.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 339534 sequences (50212058 bp) in 1.422 CPU sec, 1.516 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284596, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 332, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (354.99, 164.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675474 reads in 133.039 CPU sec, 4.292 real sec
[M::mem_process_seqs] Processed 675324 reads in 84.370 CPU sec, 2.718 real sec
sorting block 143, #reads = 1081030 mem = 536880903 peak = 1042006016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 143258, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 327, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (350.17, 162.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 339534 reads in 39.884 CPU sec, 1.337 real sec
sorting block 144, #reads = 1077977 mem = 536879059 peak = 1042006016
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 155827476
overall: 69830189056 mem 20146.850 user 478.737 sys 679.519 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_d0M-T1-TDNA-1_A23MHWWLT4_1.L001\tPU:L001\tSM:659_d0M-T1-TDNA-1_A23MHWWLT4_1\tLB:659_d0M-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_d0M-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_d0M-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 679.556 sec; CPU: 20625.591 sec
sorting block 145, #reads = 40784 mem = 20335326 peak = 1042006016
uniq header count 1
merging 146 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 8237092700
output reads: 156587089
bam_mem_sort: 146 calls 80.381 user 1.474 sys 82.357 real
bam_write: 146 calls 34.857 user 1.990 sys 39.624 real
execute: 1 calls 32.267 user 2.152 sys 725.785 real
merge_files: 1 calls 32.242 user 2.135 sys 46.190 real
parse_chunk: 2321 calls 93.432 user 22.937 sys 119.129 real
read_chunk: 6252 calls 47.727 user 49.977 sys 622.930 real
sort_block: 1 calls 34.634 user 49.330 sys 602.338 real
write_chunk: 3929 calls 31.898 user 1.839 sys 45.303 real
overall: 3191205888 mem 1867.516 user 90.495 sys 725.806 real
ls: cannot access '*.cram': No such file or directory