File Info

Filename
.command.log
Full Path
s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/3a/bea3bddae5ad28e805779332360394/.command.log
Size
142.8 KB
Task
DAQ:SENTIEON_BWAMEM (1029_N5D-T1-TDNA-1_A23MHWWLT4_1;L002;0001)
Attempt

Content

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  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/3a/bea3bddae5ad28e805779332360394/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b7/d15dc548b8718058bb1d76329b62ad/1029_N5D-T1-TDNA-1_A23MHWWLT4_1/0001.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b7/d15dc548b8718058bb1d76329b62ad/1029_N5D-T1-TDNA-1_A23MHWWLT4_1/0001.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/3a/bea3bddae5ad28e805779332360394/.command.run
==> STAGING COMPLETE (7 inputs)

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1029_N5D-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1623.459 CPU sec, 1192.915 real sec
[M::process] read 685538 sequences (100000215 bp) in 1.001 CPU sec, 1.003 real sec...
[M::process] read 683798 sequences (100000143 bp) in 1.053 CPU sec, 1.067 real sec...
[M::process] read 683234 sequences (100000248 bp) in 1.108 CPU sec, 1.112 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 279073, 6, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 260, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (276.72, 154.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 319, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (337.61, 164.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 354, 735)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1785)
[M::mem_pestat] mean and std.dev: (416.56, 340.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2310)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682426 sequences (100000019 bp) in 1.098 CPU sec, 1.111 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 278641, 9, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (233, 316, 565)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1229)
[M::mem_pestat] mean and std.dev: (339.18, 192.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1561)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 331, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (347.29, 168.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 244, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (264.42, 115.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 278072, 9, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 240, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (292.97, 171.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 337, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (352.86, 170.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 243, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (257.42, 133.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685538 reads in 219.736 CPU sec, 7.054 real sec
[M::mem_process_seqs] Processed 683798 reads in 166.190 CPU sec, 5.400 real sec
[M::mem_process_seqs] Processed 683234 reads in 182.492 CPU sec, 5.938 real sec
sorting block 0, #reads = 1071816 mem = 536879296 peak = 599875584
[M::process] read 681904 sequences (100000235 bp) in 1.881 CPU sec, 2.041 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 278262, 7, 48)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 280, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (316.80, 184.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1078)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 340, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (356.33, 171.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 339, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (353.17, 210.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682156 sequences (100000285 bp) in 1.315 CPU sec, 1.328 real sec...
[M::process] read 682270 sequences (100000127 bp) in 1.180 CPU sec, 1.181 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 277957, 7, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 264, 569)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1389)
[M::mem_pestat] mean and std.dev: (276.67, 167.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1799)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 342, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (359.15, 172.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 407, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (362.35, 123.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1092)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682426 reads in 154.082 CPU sec, 4.897 real sec
sorting block 1, #reads = 1070324 mem = 536880386 peak = 930177024
[M::mem_process_seqs] Processed 681904 reads in 99.159 CPU sec, 3.149 real sec
sorting block 2, #reads = 1070129 mem = 536882184 peak = 983793664
[M::process] read 682040 sequences (100000232 bp) in 1.624 CPU sec, 1.711 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 277461, 7, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 227, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (235.66, 99.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 782)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 340, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (356.76, 171.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 287, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (313.37, 217.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681304 sequences (100000157 bp) in 1.293 CPU sec, 1.305 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 277808, 6, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (205, 283, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (295.19, 164.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 337, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (353.13, 169.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 230, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (288.50, 165.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 277640, 9, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 280, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1143)
[M::mem_pestat] mean and std.dev: (365.06, 262.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1463)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 339, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (355.79, 170.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 252, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (287.19, 186.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682156 reads in 221.430 CPU sec, 7.086 real sec
[M::mem_process_seqs] Processed 682270 reads in 165.924 CPU sec, 5.330 real sec
[M::mem_process_seqs] Processed 682040 reads in 181.164 CPU sec, 5.838 real sec
sorting block 3, #reads = 1069802 mem = 536879161 peak = 983793664
[M::process] read 680852 sequences (100000294 bp) in 2.019 CPU sec, 2.177 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 277349, 4, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 205, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (276.20, 167.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 341, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (357.13, 169.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 269, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (67, 437)
[M::mem_pestat] mean and std.dev: (242.59, 52.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 511)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 4, #reads = 1070227 mem = 536879006 peak = 994611200
[M::process] read 680974 sequences (100000048 bp) in 1.492 CPU sec, 1.532 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 277035, 6, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 230, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (278.67, 154.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 343, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (358.27, 168.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 206, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (227.63, 100.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680042 sequences (100000168 bp) in 1.270 CPU sec, 1.270 real sec...
[M::mem_process_seqs] Processed 681304 reads in 156.890 CPU sec, 4.985 real sec
[M::mem_process_seqs] Processed 680852 reads in 99.829 CPU sec, 3.158 real sec
sorting block 5, #reads = 1069947 mem = 536878488 peak = 994611200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 277519, 8, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 214, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575)
[M::mem_pestat] mean and std.dev: (215.30, 102.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 723)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 341, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (356.86, 168.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (235.97, 99.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682342 sequences (100000215 bp) in 1.804 CPU sec, 1.910 real sec...
[M::process] read 684166 sequences (100000283 bp) in 1.310 CPU sec, 1.310 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 277276, 6, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 250, 698)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1776)
[M::mem_pestat] mean and std.dev: (375.62, 342.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2315)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 349, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (364.11, 171.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 268, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1071)
[M::mem_pestat] mean and std.dev: (347.36, 193.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1363)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680974 reads in 151.240 CPU sec, 4.817 real sec
[M::mem_process_seqs] Processed 680042 reads in 96.332 CPU sec, 3.071 real sec
sorting block 6, #reads = 1070241 mem = 536879945 peak = 994611200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 278271, 6, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (199, 287, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (280.87, 119.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 338, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (355.40, 171.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 242, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (289.19, 153.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682988 sequences (100000289 bp) in 1.811 CPU sec, 1.939 real sec...
[M::process] read 682756 sequences (100000004 bp) in 1.310 CPU sec, 1.316 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 278436, 9, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (226, 295, 559)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1225)
[M::mem_pestat] mean and std.dev: (371.97, 231.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1558)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 330, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (347.87, 170.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 232, 578)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1324)
[M::mem_pestat] mean and std.dev: (309.11, 201.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1697)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682342 reads in 151.526 CPU sec, 4.827 real sec
sorting block 7, #reads = 1069736 mem = 536880035 peak = 994611200
[M::mem_process_seqs] Processed 684166 reads in 97.033 CPU sec, 3.091 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278618, 10, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 287, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (268.29, 112.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 338, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (354.97, 172.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (240, 640, 1088)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2784)
[M::mem_pestat] mean and std.dev: (663.20, 429.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3632)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 313, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (276.41, 135.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 682084 sequences (100000197 bp) in 2.152 CPU sec, 2.269 real sec...
[M::process] read 681726 sequences (100000052 bp) in 1.360 CPU sec, 1.364 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 277741, 3, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 271, 620)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1490)
[M::mem_pestat] mean and std.dev: (398.38, 329.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1925)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 342, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (357.77, 173.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 403, 545)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1141)
[M::mem_pestat] mean and std.dev: (431.77, 256.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1456)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682988 reads in 150.912 CPU sec, 4.798 real sec
sorting block 8, #reads = 1072963 mem = 536878224 peak = 994611200
[M::mem_process_seqs] Processed 682756 reads in 96.852 CPU sec, 3.085 real sec
sorting block 9, #reads = 1072969 mem = 536882078 peak = 1035644928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 278354, 7, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 236, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055)
[M::mem_pestat] mean and std.dev: (273.04, 173.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1363)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 346, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (361.40, 173.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 247, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (242.25, 100.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682166 sequences (100000112 bp) in 2.031 CPU sec, 2.158 real sec...
[M::process] read 682442 sequences (100000295 bp) in 1.410 CPU sec, 1.413 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 277483, 4, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 312, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (274.55, 122.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 346, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (362.24, 174.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 282, 787)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1959)
[M::mem_pestat] mean and std.dev: (424.31, 332.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2545)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682084 reads in 152.653 CPU sec, 4.856 real sec
[M::mem_process_seqs] Processed 681726 reads in 97.976 CPU sec, 3.114 real sec
sorting block 10, #reads = 1071931 mem = 536878830 peak = 1035644928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 277730, 5, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 277, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (275.73, 134.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 340, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (358.05, 173.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 274, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053)
[M::mem_pestat] mean and std.dev: (304.28, 182.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1354)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682108 sequences (100000096 bp) in 2.240 CPU sec, 2.366 real sec...
[M::process] read 681788 sequences (100000288 bp) in 1.430 CPU sec, 1.428 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 277682, 7, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 264, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (245.45, 104.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 339, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (356.62, 173.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 300, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (265.38, 104.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682166 reads in 154.265 CPU sec, 4.903 real sec
[M::mem_process_seqs] Processed 682442 reads in 99.255 CPU sec, 3.162 real sec
sorting block 11, #reads = 1070362 mem = 536878609 peak = 1035644928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 277998, 9, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 445, 796)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2034)
[M::mem_pestat] mean and std.dev: (536.63, 441.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2653)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 340, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (356.76, 171.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 275, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (276.50, 136.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 889)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681448 sequences (100000070 bp) in 2.072 CPU sec, 2.202 real sec...
[M::process] read 680912 sequences (100000024 bp) in 1.410 CPU sec, 1.418 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 277673, 7, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 289, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (290.93, 141.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 340, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (357.59, 172.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 301, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (306.34, 127.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682108 reads in 153.630 CPU sec, 4.892 real sec
sorting block 12, #reads = 1070737 mem = 536882520 peak = 1035644928
[M::mem_process_seqs] Processed 681788 reads in 98.674 CPU sec, 3.140 real sec
sorting block 13, #reads = 1071124 mem = 536881113 peak = 1035644928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 278568, 9, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 237, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541)
[M::mem_pestat] mean and std.dev: (218.81, 85.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 667)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 343, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (359.92, 172.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 238, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051)
[M::mem_pestat] mean and std.dev: (307.40, 206.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1347)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680428 sequences (100000274 bp) in 2.393 CPU sec, 2.530 real sec...
[M::process] read 684028 sequences (100000145 bp) in 1.440 CPU sec, 1.445 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 277628, 11, 48)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (228, 282, 1282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3390)
[M::mem_pestat] mean and std.dev: (591.88, 664.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4444)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 345, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (360.78, 171.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (474, 2597, 2604)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6864)
[M::mem_pestat] mean and std.dev: (1959.27, 1535.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8994)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 267, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690)
[M::mem_pestat] mean and std.dev: (257.95, 107.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681448 reads in 152.648 CPU sec, 4.850 real sec
[M::mem_process_seqs] Processed 680912 reads in 97.764 CPU sec, 3.104 real sec
sorting block 14, #reads = 1070265 mem = 536879206 peak = 1035644928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 278098, 8, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 353, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (370.03, 218.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 351, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (366.66, 174.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 291, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (308.08, 120.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684236 sequences (100000073 bp) in 2.174 CPU sec, 2.277 real sec...
[M::process] read 682824 sequences (100000198 bp) in 1.530 CPU sec, 1.524 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 279095, 8, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 216, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (213.27, 90.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 329, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (346.66, 168.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 430, 664)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1618)
[M::mem_pestat] mean and std.dev: (368.52, 198.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2095)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680428 reads in 150.972 CPU sec, 4.801 real sec
[M::mem_process_seqs] Processed 684028 reads in 99.041 CPU sec, 3.138 real sec
sorting block 15, #reads = 1069406 mem = 536884846 peak = 1035644928
[M::process] read 682602 sequences (100000098 bp) in 2.388 CPU sec, 2.520 real sec...
[M::process] read 681834 sequences (100000282 bp) in 1.430 CPU sec, 1.429 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278851, 3, 50)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 202, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1165)
[M::mem_pestat] mean and std.dev: (261.81, 157.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1496)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 336, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (350.45, 166.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 303, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (272.23, 111.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682824 reads in 88.798 CPU sec, 2.815 real sec
sorting block 16, #reads = 1071441 mem = 536884085 peak = 1035644928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 278742, 8, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 242, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (263.38, 113.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1101)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 329, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (344.90, 166.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 339, 1395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3799)
[M::mem_pestat] mean and std.dev: (601.87, 681.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5001)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278369, 5, 48)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 247, 948)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2542)
[M::mem_pestat] mean and std.dev: (398.53, 339.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3339)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 338, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (352.09, 167.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 296, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579)
[M::mem_pestat] mean and std.dev: (269.62, 92.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 705)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681756 sequences (100000040 bp) in 1.609 CPU sec, 1.619 real sec...
[M::mem_process_seqs] Processed 684236 reads in 237.052 CPU sec, 7.544 real sec
[M::mem_process_seqs] Processed 682602 reads in 114.573 CPU sec, 3.665 real sec
sorting block 17, #reads = 1070568 mem = 536880327 peak = 1035644928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 277905, 13, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 252, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1069)
[M::mem_pestat] mean and std.dev: (317.02, 201.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1366)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 344, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (358.54, 170.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (113, 563, 1103)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3083)
[M::mem_pestat] mean and std.dev: (778.38, 704.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4073)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 286, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (262.55, 105.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1083)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 681958 sequences (100000171 bp) in 2.333 CPU sec, 2.492 real sec...
[M::process] read 681788 sequences (100000292 bp) in 1.413 CPU sec, 1.416 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 277861, 10, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 201, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (292.97, 196.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 344, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (359.58, 171.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (656, 726, 6228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17372)
[M::mem_pestat] mean and std.dev: (2303.50, 2572.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 22944)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 321, 628)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1532)
[M::mem_pestat] mean and std.dev: (395.05, 262.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1984)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681834 reads in 158.482 CPU sec, 5.045 real sec
[M::mem_process_seqs] Processed 681756 reads in 101.856 CPU sec, 3.232 real sec
sorting block 18, #reads = 1071200 mem = 536879362 peak = 1035644928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 277492, 11, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 268, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (330.71, 190.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 339, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (355.77, 170.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (777, 5083, 7185)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 20001)
[M::mem_pestat] mean and std.dev: (3960.27, 3209.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 26409)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 255, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (293.06, 133.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 681518 sequences (100000031 bp) in 2.352 CPU sec, 2.487 real sec...
[M::process] read 681358 sequences (100000177 bp) in 1.430 CPU sec, 1.432 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 277790, 6, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 275, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1173)
[M::mem_pestat] mean and std.dev: (308.89, 167.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1504)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 339, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (356.29, 170.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 313, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1194)
[M::mem_pestat] mean and std.dev: (332.76, 195.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1541)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681958 reads in 157.706 CPU sec, 5.011 real sec
sorting block 19, #reads = 1069671 mem = 536875922 peak = 1035644928
[M::mem_process_seqs] Processed 681788 reads in 100.734 CPU sec, 3.201 real sec
sorting block 20, #reads = 1070129 mem = 536880455 peak = 1045213184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 277207, 6, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 282, 534)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1346)
[M::mem_pestat] mean and std.dev: (344.53, 288.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1752)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 340, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (357.00, 170.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 262, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (265.71, 83.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681098 sequences (100000228 bp) in 2.014 CPU sec, 2.133 real sec...
[M::process] read 680432 sequences (100000102 bp) in 1.420 CPU sec, 1.417 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 277539, 14, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 191, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (232.53, 126.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1080)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 343, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (358.94, 171.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (242, 715, 2924)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8288)
[M::mem_pestat] mean and std.dev: (1361.14, 1391.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10970)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 242, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076)
[M::mem_pestat] mean and std.dev: (298.28, 218.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1382)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681518 reads in 160.919 CPU sec, 5.130 real sec
[M::mem_process_seqs] Processed 681358 reads in 101.766 CPU sec, 3.237 real sec
sorting block 21, #reads = 1069841 mem = 536881543 peak = 1045213184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 277400, 5, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 249, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (294.11, 143.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 346, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (362.19, 173.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 335, 751)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1879)
[M::mem_pestat] mean and std.dev: (425.11, 362.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2443)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681504 sequences (100000181 bp) in 2.338 CPU sec, 2.467 real sec...
[M::process] read 684624 sequences (100000153 bp) in 1.450 CPU sec, 1.449 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 277979, 6, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (201, 319, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (311.96, 146.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 350, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (365.98, 173.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 314, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (304.68, 133.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681098 reads in 155.846 CPU sec, 4.954 real sec
[M::mem_process_seqs] Processed 680432 reads in 97.913 CPU sec, 3.112 real sec
sorting block 22, #reads = 1070744 mem = 536887149 peak = 1045213184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 277769, 8, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 204, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (236.22, 101.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 344, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (360.20, 172.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 259, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (293.58, 138.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683808 sequences (100000094 bp) in 2.148 CPU sec, 2.282 real sec...
[M::process] read 683208 sequences (100000018 bp) in 1.430 CPU sec, 1.436 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 278330, 6, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 339, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1183)
[M::mem_pestat] mean and std.dev: (301.50, 153.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1522)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 327, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (346.93, 171.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 256, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (279.82, 112.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681504 reads in 153.564 CPU sec, 4.889 real sec
sorting block 23, #reads = 1070671 mem = 536878476 peak = 1045213184
[M::mem_process_seqs] Processed 684624 reads in 98.915 CPU sec, 3.141 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 278352, 11, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 239, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (254.30, 126.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1300)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 334, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (351.96, 172.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (262, 1632, 3718)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10630)
[M::mem_pestat] mean and std.dev: (1735.18, 1613.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14086)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 300, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (296.35, 159.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 682878 sequences (100000093 bp) in 2.373 CPU sec, 2.501 real sec...
[M::process] read 682110 sequences (100000090 bp) in 1.420 CPU sec, 1.422 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 278407, 10, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 280, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623)
[M::mem_pestat] mean and std.dev: (248.20, 109.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 764)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 338, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (355.14, 172.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (608, 1572, 1572)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3500)
[M::mem_pestat] mean and std.dev: (1150.80, 700.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4464)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 283, 585)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1277)
[M::mem_pestat] mean and std.dev: (389.33, 264.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1623)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683808 reads in 154.358 CPU sec, 4.903 real sec
sorting block 24, #reads = 1072233 mem = 536880772 peak = 1045213184
[M::mem_process_seqs] Processed 683208 reads in 98.738 CPU sec, 3.136 real sec
sorting block 25, #reads = 1072651 mem = 536880088 peak = 1088352256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 278189, 3, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 256, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088)
[M::mem_pestat] mean and std.dev: (338.15, 222.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1387)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 337, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (354.19, 171.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 219, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581)
[M::mem_pestat] mean and std.dev: (203.07, 70.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682118 sequences (100000109 bp) in 2.210 CPU sec, 2.310 real sec...
[M::process] read 682120 sequences (100000139 bp) in 1.440 CPU sec, 1.439 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 277795, 6, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 263, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690)
[M::mem_pestat] mean and std.dev: (286.18, 152.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 897)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 343, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (358.95, 171.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 303, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (345.24, 189.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682878 reads in 155.985 CPU sec, 4.961 real sec
[M::mem_process_seqs] Processed 682110 reads in 100.498 CPU sec, 3.190 real sec
sorting block 26, #reads = 1071788 mem = 536878066 peak = 1088352256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 277214, 3, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 276, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1148)
[M::mem_pestat] mean and std.dev: (314.72, 195.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1469)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 342, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (357.67, 170.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 253, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (272.65, 113.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682018 sequences (100000166 bp) in 2.661 CPU sec, 2.780 real sec...
[M::process] read 681906 sequences (100000051 bp) in 1.430 CPU sec, 1.432 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 277716, 3, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 324, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (292.03, 136.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 339, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (355.15, 169.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 288, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (322.78, 150.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682118 reads in 159.883 CPU sec, 5.085 real sec
[M::mem_process_seqs] Processed 682120 reads in 101.914 CPU sec, 3.240 real sec
sorting block 27, #reads = 1070741 mem = 536878982 peak = 1088352256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 277539, 10, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 236, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (243.29, 122.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 339, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (355.01, 169.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 113, 1234)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3650)
[M::mem_pestat] mean and std.dev: (285.00, 424.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4858)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 285, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (254.94, 111.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1100)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 681428 sequences (100000272 bp) in 2.147 CPU sec, 2.259 real sec...
[M::process] read 680946 sequences (100000139 bp) in 1.420 CPU sec, 1.423 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 277732, 7, 59)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 235, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (283.29, 160.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 340, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (355.11, 168.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 302, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1251)
[M::mem_pestat] mean and std.dev: (353.62, 221.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1610)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682018 reads in 159.449 CPU sec, 5.078 real sec
sorting block 28, #reads = 1070860 mem = 536879632 peak = 1088352256
[M::mem_process_seqs] Processed 681906 reads in 101.402 CPU sec, 3.222 real sec
sorting block 29, #reads = 1069839 mem = 536885612 peak = 1088352256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 277341, 8, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 283, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (292.75, 155.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 340, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (356.16, 168.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 234, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 493)
[M::mem_pestat] mean and std.dev: (226.80, 63.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 590)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680576 sequences (100000134 bp) in 2.245 CPU sec, 2.365 real sec...
[M::process] read 686192 sequences (100000162 bp) in 1.480 CPU sec, 1.474 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 277585, 11, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (219, 287, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (286.43, 110.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 341, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (356.48, 167.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (777, 1004, 2649)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6393)
[M::mem_pestat] mean and std.dev: (1424.91, 963.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8265)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 329, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (323.54, 118.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681428 reads in 156.885 CPU sec, 4.984 real sec
[M::mem_process_seqs] Processed 680946 reads in 99.573 CPU sec, 3.170 real sec
sorting block 30, #reads = 1068632 mem = 536876547 peak = 1088352256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278056, 9, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (237, 313, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759)
[M::mem_pestat] mean and std.dev: (310.66, 116.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 349, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (363.68, 171.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 266, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (289.35, 137.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686314 sequences (100000280 bp) in 2.116 CPU sec, 2.252 real sec...
[M::process] read 685178 sequences (100000006 bp) in 1.440 CPU sec, 1.442 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 279496, 9, 50)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 282, 964)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2514)
[M::mem_pestat] mean and std.dev: (465.38, 416.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3289)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 320, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (343.47, 173.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 321, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (344.26, 195.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680576 reads in 155.561 CPU sec, 4.948 real sec
[M::mem_process_seqs] Processed 686192 reads in 102.404 CPU sec, 3.260 real sec
sorting block 31, #reads = 1069180 mem = 536878531 peak = 1088352256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 280040, 8, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 164, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (222.95, 132.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 319, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (342.23, 172.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 303, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (295.56, 156.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684068 sequences (100000234 bp) in 2.505 CPU sec, 2.635 real sec...
[M::process] read 683368 sequences (100000165 bp) in 1.440 CPU sec, 1.444 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 279803, 6, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 264, 610)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1464)
[M::mem_pestat] mean and std.dev: (280.08, 170.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1891)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 328, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (348.28, 173.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 279, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050)
[M::mem_pestat] mean and std.dev: (310.78, 212.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1350)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686314 reads in 160.829 CPU sec, 5.110 real sec
sorting block 32, #reads = 1070065 mem = 536880473 peak = 1088352256
[M::process] read 682944 sequences (100000226 bp) in 1.602 CPU sec, 1.608 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 279276, 5, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 230, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (271.21, 142.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 335, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (354.09, 174.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 317, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (319.51, 137.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684068 reads in 91.983 CPU sec, 2.953 real sec
[M::mem_process_seqs] Processed 685178 reads in 217.434 CPU sec, 6.941 real sec
sorting block 33, #reads = 1071341 mem = 536876644 peak = 1088352256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 277942, 7, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 262, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1078)
[M::mem_pestat] mean and std.dev: (320.89, 189.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1373)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 339, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (356.99, 174.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 286, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (292.51, 133.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682440 sequences (100000089 bp) in 2.269 CPU sec, 2.404 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 277876, 8, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 264, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (286.38, 172.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 342, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (359.73, 175.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 435, 910)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2282)
[M::mem_pestat] mean and std.dev: (526.64, 440.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2968)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682340 sequences (100000150 bp) in 1.420 CPU sec, 1.419 real sec...
[M::mem_process_seqs] Processed 683368 reads in 178.483 CPU sec, 5.678 real sec
sorting block 34, #reads = 1070568 mem = 536878974 peak = 1088352256
[M::mem_process_seqs] Processed 682944 reads in 106.324 CPU sec, 3.373 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 278379, 13, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 220, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (253.29, 146.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 342, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (358.82, 173.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (587, 754, 2021)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4889)
[M::mem_pestat] mean and std.dev: (991.23, 635.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6323)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 304, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (262.82, 108.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 682230 sequences (100000036 bp) in 2.510 CPU sec, 2.671 real sec...
[M::process] read 682088 sequences (100000143 bp) in 1.400 CPU sec, 1.403 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 278280, 5, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 268, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (309.21, 149.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 341, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (357.61, 172.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 294, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (269.47, 105.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682440 reads in 163.352 CPU sec, 5.191 real sec
sorting block 35, #reads = 1067342 mem = 536884191 peak = 1088352256
[M::process] read 682236 sequences (100000118 bp) in 1.710 CPU sec, 1.711 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278104, 6, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 261, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (260.60, 119.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1061)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 342, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (357.84, 171.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 358, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1091)
[M::mem_pestat] mean and std.dev: (304.45, 138.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1388)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682230 reads in 95.802 CPU sec, 3.266 real sec
sorting block 36, #reads = 1068176 mem = 536877698 peak = 1088352256
[M::mem_process_seqs] Processed 682340 reads in 219.775 CPU sec, 7.220 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 277964, 8, 64)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 235, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (234.03, 94.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 338, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (354.84, 169.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 396, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (356.30, 111.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681810 sequences (100000039 bp) in 2.313 CPU sec, 2.433 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 278269, 1, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 253, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (263.40, 122.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 338, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (354.55, 169.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 240, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (269.14, 163.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 688830 sequences (100000129 bp) in 1.500 CPU sec, 1.500 real sec...
[M::mem_process_seqs] Processed 682088 reads in 178.986 CPU sec, 5.690 real sec
sorting block 37, #reads = 1067040 mem = 536879832 peak = 1088352256
[M::mem_process_seqs] Processed 682236 reads in 104.705 CPU sec, 3.316 real sec
sorting block 38, #reads = 1067039 mem = 536884542 peak = 1088352256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278400, 2, 48)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (212, 341, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (307.74, 128.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 342, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (360.22, 174.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 259, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (270.58, 123.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1103)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687216 sequences (100000243 bp) in 2.189 CPU sec, 2.326 real sec...
[M::process] read 687102 sequences (100000075 bp) in 1.510 CPU sec, 1.508 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 281104, 7, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 269, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (274.53, 129.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1077)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 303, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (328.52, 167.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 334, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (300.97, 147.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681810 reads in 162.759 CPU sec, 5.183 real sec
[M::process] read 685320 sequences (100000016 bp) in 1.877 CPU sec, 1.883 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 280587, 10, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 315, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (287.34, 123.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 314, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (334.99, 167.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (582, 582, 840)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (66, 1356)
[M::mem_pestat] mean and std.dev: (641.89, 230.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1614)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 326, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (288.50, 140.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687216 reads in 89.006 CPU sec, 2.820 real sec
sorting block 39, #reads = 1066728 mem = 536880080 peak = 1088352256
[M::mem_process_seqs] Processed 688830 reads in 224.070 CPU sec, 7.132 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 280856, 7, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 219, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (259.72, 131.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 314, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (335.20, 166.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 239, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (285.68, 117.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 968)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 40, #reads = 1068495 mem = 536878642 peak = 1088352256
[M::process] read 684532 sequences (100000187 bp) in 2.156 CPU sec, 2.267 real sec...
[M::process] read 683540 sequences (100000121 bp) in 1.450 CPU sec, 1.450 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 280113, 12, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 279, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (318.27, 199.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 326, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (344.68, 169.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (357, 1980, 3414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9528)
[M::mem_pestat] mean and std.dev: (1711.58, 1554.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12585)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (125, 218, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (212.83, 109.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687102 reads in 182.449 CPU sec, 5.802 real sec
[M::mem_process_seqs] Processed 685320 reads in 105.951 CPU sec, 3.359 real sec
sorting block 41, #reads = 1066717 mem = 536878331 peak = 1088352256
[M::process] read 682934 sequences (100000243 bp) in 2.330 CPU sec, 2.486 real sec...
[M::process] read 682518 sequences (100000252 bp) in 1.420 CPU sec, 1.426 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 279228, 13, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 220, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (224.32, 76.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 337, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (354.80, 173.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (270, 557, 7144)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 20892)
[M::mem_pestat] mean and std.dev: (2487.31, 3112.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 27766)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 243, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (249.69, 103.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 744)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683540 reads in 95.424 CPU sec, 3.038 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 279994, 4, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 222, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (234.54, 105.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 332, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (349.34, 171.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 303, 639)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1479)
[M::mem_pestat] mean and std.dev: (370.38, 248.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1899)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682092 sequences (100000241 bp) in 1.528 CPU sec, 1.532 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 278991, 7, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 208, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (248.67, 127.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 339, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (356.78, 173.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 290, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (329.93, 182.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684532 reads in 254.356 CPU sec, 8.098 real sec
sorting block 42, #reads = 1066746 mem = 536884221 peak = 1088352256
[M::process] read 681736 sequences (100000039 bp) in 1.592 CPU sec, 1.594 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 278738, 5, 53)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (224, 297, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (279.88, 112.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 780)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 341, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (359.32, 174.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 267, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (273.08, 116.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682518 reads in 92.150 CPU sec, 2.929 real sec
sorting block 43, #reads = 1067107 mem = 536883607 peak = 1110978560
[M::mem_process_seqs] Processed 682934 reads in 243.357 CPU sec, 7.777 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 278419, 9, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (212, 252, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1067)
[M::mem_pestat] mean and std.dev: (329.96, 238.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1352)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 343, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (361.69, 176.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 298, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (299.53, 138.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681700 sequences (100000141 bp) in 2.123 CPU sec, 2.254 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 278645, 3, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 201, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (215.83, 79.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 860)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 348, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (366.58, 179.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 282, 563)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1285)
[M::mem_pestat] mean and std.dev: (354.56, 221.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1646)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686604 sequences (100000033 bp) in 1.480 CPU sec, 1.474 real sec...
[M::mem_process_seqs] Processed 682092 reads in 177.160 CPU sec, 5.632 real sec
sorting block 44, #reads = 1066855 mem = 536882442 peak = 1110978560
[M::mem_process_seqs] Processed 681736 reads in 103.507 CPU sec, 3.283 real sec
sorting block 45, #reads = 1067220 mem = 536879067 peak = 1123672064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 278846, 10, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 225, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559)
[M::mem_pestat] mean and std.dev: (241.45, 96.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 685)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 351, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050)
[M::mem_pestat] mean and std.dev: (371.39, 184.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1326)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1037, 1692, 2510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5456)
[M::mem_pestat] mean and std.dev: (1846.70, 1145.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6929)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 201, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610)
[M::mem_pestat] mean and std.dev: (247.03, 97.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 751)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 685704 sequences (100000000 bp) in 2.458 CPU sec, 2.592 real sec...
[M::process] read 685274 sequences (100000183 bp) in 1.430 CPU sec, 1.433 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 280056, 4, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 207, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (217.72, 96.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 951)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 317, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (341.34, 173.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 349, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050)
[M::mem_pestat] mean and std.dev: (310.62, 168.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1341)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681700 reads in 164.923 CPU sec, 5.242 real sec
[M::mem_process_seqs] Processed 686604 reads in 108.922 CPU sec, 3.463 real sec
sorting block 46, #reads = 1064124 mem = 536876795 peak = 1123672064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 280125, 4, 53)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 206, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (269.97, 160.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 322, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (341.11, 168.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 293, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (288.91, 100.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684264 sequences (100000100 bp) in 2.341 CPU sec, 2.434 real sec...
[M::process] read 684214 sequences (100000221 bp) in 1.420 CPU sec, 1.423 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 280035, 9, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 230, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (267.00, 132.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 893)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 324, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (343.10, 168.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 340, 749)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1761)
[M::mem_pestat] mean and std.dev: (490.46, 366.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2267)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685704 reads in 154.850 CPU sec, 4.919 real sec
[M::mem_process_seqs] Processed 685274 reads in 99.951 CPU sec, 3.179 real sec
sorting block 47, #reads = 1069979 mem = 536878831 peak = 1123672064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 279357, 1, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 233, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (252.44, 114.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 332, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (350.30, 171.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 330, 589)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1437)
[M::mem_pestat] mean and std.dev: (327.33, 220.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1861)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683780 sequences (100000011 bp) in 2.359 CPU sec, 2.465 real sec...
[M::process] read 683004 sequences (100000079 bp) in 1.400 CPU sec, 1.404 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 279177, 6, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (205, 302, 749)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1837)
[M::mem_pestat] mean and std.dev: (491.23, 408.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2381)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 334, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (349.89, 170.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 444, 584)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1318)
[M::mem_pestat] mean and std.dev: (386.89, 212.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1685)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684264 reads in 161.271 CPU sec, 5.129 real sec
sorting block 48, #reads = 1069287 mem = 536881861 peak = 1123672064
[M::mem_process_seqs] Processed 684214 reads in 103.739 CPU sec, 3.298 real sec
sorting block 49, #reads = 1069527 mem = 536879072 peak = 1123672064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 278578, 3, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 267, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (243.78, 158.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 337, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (353.90, 172.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 225, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (221.38, 79.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682344 sequences (100000177 bp) in 2.379 CPU sec, 2.482 real sec...
[M::process] read 682276 sequences (100000141 bp) in 1.440 CPU sec, 1.436 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 278088, 4, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 257, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (270.64, 122.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 340, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (355.52, 171.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 251, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (278.48, 110.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683780 reads in 163.879 CPU sec, 5.211 real sec
[M::mem_process_seqs] Processed 683004 reads in 107.611 CPU sec, 3.421 real sec
sorting block 50, #reads = 1067156 mem = 536877286 peak = 1123672064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 277962, 7, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (214, 343, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1069)
[M::mem_pestat] mean and std.dev: (326.26, 148.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1354)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 339, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (356.44, 171.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 323, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (270.41, 110.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682496 sequences (100000095 bp) in 2.420 CPU sec, 2.514 real sec...
[M::process] read 682488 sequences (100000051 bp) in 1.410 CPU sec, 1.416 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 278078, 15, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (220, 316, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1129)
[M::mem_pestat] mean and std.dev: (288.66, 131.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1432)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 338, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (356.45, 172.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (27, 55, 648)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1890)
[M::mem_pestat] mean and std.dev: (291.50, 305.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2511)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 294, 721)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1707)
[M::mem_pestat] mean and std.dev: (357.12, 256.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2200)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682344 reads in 164.926 CPU sec, 5.243 real sec
[M::mem_process_seqs] Processed 682276 reads in 106.481 CPU sec, 3.393 real sec
sorting block 51, #reads = 1065710 mem = 536879114 peak = 1123672064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 277922, 3, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 201, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (237.24, 137.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 967)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 338, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (356.12, 172.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 292, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (315.68, 157.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684732 sequences (100000236 bp) in 2.432 CPU sec, 2.521 real sec...
[M::process] read 689906 sequences (100000236 bp) in 1.450 CPU sec, 1.455 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 278435, 5, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (242, 373, 1236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3224)
[M::mem_pestat] mean and std.dev: (727.48, 773.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4218)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 341, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (359.38, 174.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 274, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (257.63, 103.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 901)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682496 reads in 166.974 CPU sec, 5.308 real sec
sorting block 52, #reads = 1064663 mem = 536877875 peak = 1123672064
[M::mem_process_seqs] Processed 682488 reads in 106.743 CPU sec, 3.400 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278940, 4, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 234, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (248.33, 114.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 328, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (350.89, 175.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 240, 584)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1366)
[M::mem_pestat] mean and std.dev: (293.41, 224.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1757)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 688358 sequences (100000192 bp) in 2.215 CPU sec, 2.313 real sec...
[M::process] read 688106 sequences (100000005 bp) in 1.430 CPU sec, 1.434 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281516, 6, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 299, 688)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1688)
[M::mem_pestat] mean and std.dev: (443.27, 365.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2188)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 297, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (320.68, 161.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 273, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (272.91, 109.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684732 reads in 173.871 CPU sec, 5.540 real sec
sorting block 53, #reads = 1064835 mem = 536879205 peak = 1123672064
[M::mem_process_seqs] Processed 689906 reads in 109.769 CPU sec, 3.501 real sec
sorting block 54, #reads = 1062163 mem = 536878568 peak = 1145851904
[M::process] read 686406 sequences (100000185 bp) in 2.155 CPU sec, 2.251 real sec...
[M::process] read 684552 sequences (100000008 bp) in 1.460 CPU sec, 1.460 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 280277, 13, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 366, 1936)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5462)
[M::mem_pestat] mean and std.dev: (817.62, 876.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7225)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 306, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (328.90, 164.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 370, 1940)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5784)
[M::mem_pestat] mean and std.dev: (644.62, 761.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7706)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 228, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (229.88, 77.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688106 reads in 96.961 CPU sec, 3.086 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 281809, 6, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 308, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (289.90, 176.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 305, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (327.44, 163.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 353, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873)
[M::mem_pestat] mean and std.dev: (281.76, 102.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1094)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683788 sequences (100000185 bp) in 1.472 CPU sec, 1.482 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 280162, 5, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 333, 622)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1502)
[M::mem_pestat] mean and std.dev: (361.79, 271.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1942)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 318, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (339.14, 168.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 243, 577)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1373)
[M::mem_pestat] mean and std.dev: (274.08, 154.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1771)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688358 reads in 260.144 CPU sec, 8.294 real sec
sorting block 55, #reads = 1065772 mem = 536879270 peak = 1145851904
[M::process] read 683160 sequences (100000214 bp) in 1.777 CPU sec, 1.785 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 279167, 8, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 257, 544)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1288)
[M::mem_pestat] mean and std.dev: (346.83, 259.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1660)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 329, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (348.66, 171.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (293, 393, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866)
[M::mem_pestat] mean and std.dev: (381.89, 132.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684552 reads in 96.999 CPU sec, 3.186 real sec
sorting block 56, #reads = 1066281 mem = 536880103 peak = 1145851904
[M::mem_process_seqs] Processed 686406 reads in 244.877 CPU sec, 7.924 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 278924, 4, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (208, 315, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (334.00, 175.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 334, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (350.92, 169.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 328, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (337.66, 146.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682194 sequences (100000232 bp) in 2.174 CPU sec, 2.274 real sec...
[M::process] read 681834 sequences (100000226 bp) in 1.420 CPU sec, 1.421 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 278022, 7, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (268, 353, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (333.64, 136.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 336, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (354.53, 172.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 317, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (341.48, 152.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683788 reads in 185.345 CPU sec, 5.901 real sec
sorting block 57, #reads = 1065337 mem = 536880460 peak = 1145851904
[M::mem_process_seqs] Processed 683160 reads in 107.632 CPU sec, 3.418 real sec
sorting block 58, #reads = 1064545 mem = 536879325 peak = 1145851904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 277840, 6, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 253, 676)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1758)
[M::mem_pestat] mean and std.dev: (326.28, 310.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2299)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 339, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (356.49, 171.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 380, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (344.79, 157.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681336 sequences (100000216 bp) in 2.103 CPU sec, 2.211 real sec...
[M::process] read 398312 sequences (58398731 bp) in 0.970 CPU sec, 0.975 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 277807, 3, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 243, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (232.50, 93.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 342, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (358.70, 171.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 286, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (273.26, 114.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682194 reads in 162.496 CPU sec, 5.191 real sec
[M::mem_process_seqs] Processed 681834 reads in 103.470 CPU sec, 3.305 real sec
sorting block 59, #reads = 1066513 mem = 536879021 peak = 1145851904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 278292, 10, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 299, 674)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1682)
[M::mem_pestat] mean and std.dev: (298.19, 208.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2186)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 348, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (364.53, 175.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (211, 750, 855)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2143)
[M::mem_pestat] mean and std.dev: (627.44, 427.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2787)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 278, 1124)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2998)
[M::mem_pestat] mean and std.dev: (525.80, 505.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3935)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 162676, 2, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 338, 744)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1860)
[M::mem_pestat] mean and std.dev: (524.62, 492.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2495)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 344, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (362.51, 176.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 372, 2035)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5657)
[M::mem_pestat] mean and std.dev: (1041.33, 1206.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7468)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681336 reads in 127.244 CPU sec, 4.124 real sec
[M::mem_process_seqs] Processed 398312 reads in 66.657 CPU sec, 2.158 real sec
sorting block 60, #reads = 1067373 mem = 536878829 peak = 1145851904
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 65292428
overall: 70110457856 mem 9720.115 user 266.386 sys 1469.256 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1029_N5D-T1-TDNA-1_A23MHWWLT4_1.L002\tPU:L002\tSM:1029_N5D-T1-TDNA-1_A23MHWWLT4_1\tLB:1029_N5D-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0001.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0001.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 1469.294 sec; CPU: 9986.504 sec
sorting block 61, #reads = 469746 mem = 236550903 peak = 1145851904
uniq header count 1
merging 62 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3947713685
output reads: 65676618
bam_mem_sort: 62 calls 23.363 user 0.458 sys 23.951 real
bam_write: 62 calls 53.078 user 1.257 sys 54.654 real
execute: 1 calls 12.095 user 0.867 sys 1490.958 real
merge_files: 1 calls 12.058 user 0.857 sys 20.965 real
parse_chunk: 984 calls 33.199 user 8.174 sys 42.007 real
read_chunk: 2657 calls 22.588 user 23.419 sys 276.553 real
sort_block: 1 calls 17.762 user 23.083 sys 268.981 real
write_chunk: 1671 calls 11.748 user 0.678 sys 20.293 real
overall: 2866888704 mem 794.886 user 41.024 sys 1490.983 real
ls: cannot access '*.cram': No such file or directory