Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/52/e55fac4149d69f6d32534ca3bc6896/.command.sh Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/fd/7763eaf9946751cec2ce48a4f1afc3/1136_5TB-T1-TDNA-1_A23MHWWLT4_1/0003.1136_5TB-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/52/e55fac4149d69f6d32534ca3bc6896/.command.run Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/fd/7763eaf9946751cec2ce48a4f1afc3/1136_5TB-T1-TDNA-1_A23MHWWLT4_1/0003.1136_5TB-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz ==> STAGING COMPLETE (7 inputs) This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_5TB-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2238.912 CPU sec, 109.687 real sec [M::process] read 694382 sequences (100000087 bp) in 1.437 CPU sec, 1.447 real sec... [M::process] read 695256 sequences (100000223 bp) in 1.974 CPU sec, 2.221 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 284882, 24, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 217, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940) [M::mem_pestat] mean and std.dev: (237.25, 139.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1202) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 258, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (282.17, 140.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (331, 595, 1326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3316) [M::mem_pestat] mean and std.dev: (764.83, 660.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4311) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 279, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1086) [M::mem_pestat] mean and std.dev: (339.78, 198.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1377) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695662 sequences (100000283 bp) in 1.969 CPU sec, 2.479 real sec... [M::process] read 693868 sequences (100000010 bp) in 1.976 CPU sec, 2.409 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 285260, 15, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 294, 711) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1753) [M::mem_pestat] mean and std.dev: (327.22, 212.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2274) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 255, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (279.70, 139.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (850, 1852, 2448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5644) [M::mem_pestat] mean and std.dev: (1523.57, 874.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7242) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 265, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (273.85, 171.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694382 reads in 247.467 CPU sec, 9.097 real sec [M::mem_process_seqs] Processed 695256 reads in 163.322 CPU sec, 6.162 real sec sorting block 0, #reads = 1082490 mem = 536877626 peak = 566452224 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 285037, 24, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 325, 1264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3420) [M::mem_pestat] mean and std.dev: (794.52, 891.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 253, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (277.07, 138.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (305, 755, 2213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6029) [M::mem_pestat] mean and std.dev: (1091.65, 961.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7937) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 319, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1126) [M::mem_pestat] mean and std.dev: (319.51, 177.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1429) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694836 sequences (100000104 bp) in 2.669 CPU sec, 3.207 real sec... [M::process] read 692938 sequences (100000105 bp) in 2.051 CPU sec, 2.190 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 284851, 17, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 276, 922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2398) [M::mem_pestat] mean and std.dev: (472.85, 481.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3136) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 261, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (285.28, 142.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 995) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (687, 841, 891) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (279, 1299) [M::mem_pestat] mean and std.dev: (774.36, 123.20) [M::mem_pestat] low and high boundaries for proper pairs: (75, 1503) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 355, 587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1321) [M::mem_pestat] mean and std.dev: (336.08, 203.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1688) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695662 reads in 243.727 CPU sec, 8.049 real sec [M::mem_process_seqs] Processed 693868 reads in 163.886 CPU sec, 5.561 real sec sorting block 1, #reads = 1083063 mem = 536880326 peak = 695427072 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 285013, 12, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 349, 1174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3126) [M::mem_pestat] mean and std.dev: (613.00, 662.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4102) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 255, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (279.46, 139.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1039, 1902, 5957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15793) [M::mem_pestat] mean and std.dev: (2774.33, 2202.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20711) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 305, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (317.34, 139.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693120 sequences (100000005 bp) in 2.723 CPU sec, 3.451 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 284655, 22, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 323, 622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1456) [M::mem_pestat] mean and std.dev: (388.91, 278.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1873) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 260, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (282.72, 139.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (300, 710, 1663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4389) [M::mem_pestat] mean and std.dev: (859.10, 796.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5752) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 284, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (295.07, 162.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693250 sequences (100000249 bp) in 2.211 CPU sec, 2.412 real sec... [M::mem_process_seqs] Processed 694836 reads in 255.273 CPU sec, 8.817 real sec [M::mem_process_seqs] Processed 692938 reads in 172.180 CPU sec, 6.137 real sec sorting block 2, #reads = 1082312 mem = 536876424 peak = 848711680 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 284365, 15, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 352, 1657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4553) [M::mem_pestat] mean and std.dev: (686.91, 817.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6001) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 257, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (280.59, 137.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (312, 357, 927) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2157) [M::mem_pestat] mean and std.dev: (556.21, 397.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2772) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 269, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1115) [M::mem_pestat] mean and std.dev: (282.57, 175.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1430) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692050 sequences (100000173 bp) in 3.392 CPU sec, 4.247 real sec... [M::process] read 692526 sequences (100000060 bp) in 2.580 CPU sec, 2.610 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 284107, 22, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 235, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (267.02, 139.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 989) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 257, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (279.32, 136.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (480, 1084, 2400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6240) [M::mem_pestat] mean and std.dev: (1531.95, 1425.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8160) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 303, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068) [M::mem_pestat] mean and std.dev: (306.76, 184.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1360) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693120 reads in 280.380 CPU sec, 10.572 real sec sorting block 3, #reads = 1081500 mem = 536880773 peak = 848711680 [M::mem_process_seqs] Processed 693250 reads in 184.023 CPU sec, 7.025 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 283773, 22, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 312, 657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1509) [M::mem_pestat] mean and std.dev: (434.25, 263.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1935) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 262, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (283.44, 137.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (304, 721, 1605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4207) [M::mem_pestat] mean and std.dev: (865.05, 747.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5508) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 354, 619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1421) [M::mem_pestat] mean and std.dev: (383.17, 236.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1822) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691970 sequences (100000006 bp) in 3.119 CPU sec, 3.712 real sec... sorting block 4, #reads = 1081238 mem = 536876113 peak = 848711680 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 283877, 18, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 257, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056) [M::mem_pestat] mean and std.dev: (319.00, 225.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1353) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 259, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (281.23, 136.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (292, 1804, 2429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6703) [M::mem_pestat] mean and std.dev: (1421.18, 1084.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8840) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 302, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1261) [M::mem_pestat] mean and std.dev: (377.32, 311.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1629) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695474 sequences (100000283 bp) in 2.146 CPU sec, 2.233 real sec... [M::mem_process_seqs] Processed 692050 reads in 280.961 CPU sec, 11.103 real sec [M::mem_process_seqs] Processed 692526 reads in 184.427 CPU sec, 7.361 real sec sorting block 5, #reads = 1080945 mem = 536876971 peak = 848711680 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 284008, 15, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 299, 1122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2916) [M::mem_pestat] mean and std.dev: (660.05, 698.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3813) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 262, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (285.17, 140.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (307, 511, 836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1894) [M::mem_pestat] mean and std.dev: (402.83, 231.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2423) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 325, 562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1260) [M::mem_pestat] mean and std.dev: (347.40, 254.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1609) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694894 sequences (100000052 bp) in 3.356 CPU sec, 4.028 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 285617, 13, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 383, 1706) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4696) [M::mem_pestat] mean and std.dev: (836.49, 854.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6191) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 255, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (277.37, 137.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (388, 708, 1350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3274) [M::mem_pestat] mean and std.dev: (744.08, 716.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4236) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 214, 671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1663) [M::mem_pestat] mean and std.dev: (363.28, 318.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2159) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694744 sequences (100000117 bp) in 2.705 CPU sec, 2.800 real sec... [M::mem_process_seqs] Processed 691970 reads in 279.153 CPU sec, 11.040 real sec [M::mem_process_seqs] Processed 695474 reads in 181.728 CPU sec, 7.298 real sec sorting block 6, #reads = 1081535 mem = 536878696 peak = 848711680 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 284751, 17, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 228, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1189) [M::mem_pestat] mean and std.dev: (333.40, 286.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 258, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (281.86, 140.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (486, 1620, 3065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8223) [M::mem_pestat] mean and std.dev: (1906.00, 2007.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10802) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 336, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029) [M::mem_pestat] mean and std.dev: (291.13, 112.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1298) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693812 sequences (100000054 bp) in 3.305 CPU sec, 3.836 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 285074, 21, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 270, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016) [M::mem_pestat] mean and std.dev: (319.14, 220.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1300) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 257, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (280.34, 139.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (312, 811, 1409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3603) [M::mem_pestat] mean and std.dev: (847.86, 745.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4700) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 317, 809) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2075) [M::mem_pestat] mean and std.dev: (392.50, 342.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2708) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694204 sequences (100000138 bp) in 2.394 CPU sec, 2.426 real sec... [M::mem_process_seqs] Processed 694894 reads in 274.013 CPU sec, 10.812 real sec [M::mem_process_seqs] Processed 694744 reads in 180.108 CPU sec, 7.199 real sec sorting block 7, #reads = 1083525 mem = 536878005 peak = 848711680 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 285007, 20, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 279, 573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1441) [M::mem_pestat] mean and std.dev: (312.14, 250.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1875) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 261, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (283.37, 140.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (465, 887, 2105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5385) [M::mem_pestat] mean and std.dev: (1373.90, 1329.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7025) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 341, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050) [M::mem_pestat] mean and std.dev: (334.10, 166.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1326) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692120 sequences (100000174 bp) in 3.594 CPU sec, 4.404 real sec... sorting block 8, #reads = 1083781 mem = 536878743 peak = 848711680 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 284582, 30, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 377, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1116) [M::mem_pestat] mean and std.dev: (302.00, 134.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 258, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (281.46, 139.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (513, 663, 1129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2361) [M::mem_pestat] mean and std.dev: (771.41, 483.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2977) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 325, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1092) [M::mem_pestat] mean and std.dev: (348.67, 214.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1393) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693812 reads in 278.864 CPU sec, 11.048 real sec [M::process] read 693298 sequences (100000121 bp) in 3.264 CPU sec, 3.433 real sec... [M::mem_process_seqs] Processed 694204 reads in 179.506 CPU sec, 7.003 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 284328, 12, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 287, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (236.49, 101.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 265, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (286.57, 140.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (234, 550, 2355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6597) [M::mem_pestat] mean and std.dev: (1089.08, 1323.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8718) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (304, 358, 536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000) [M::mem_pestat] mean and std.dev: (348.29, 147.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1232) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 9, #reads = 1084737 mem = 536881025 peak = 848711680 [M::process] read 693502 sequences (100000213 bp) in 3.509 CPU sec, 3.990 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 284546, 15, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (247, 334, 1320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3466) [M::mem_pestat] mean and std.dev: (701.85, 838.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 258, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (280.63, 137.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (585, 660, 1487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3291) [M::mem_pestat] mean and std.dev: (932.07, 784.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4193) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 353, 903) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2337) [M::mem_pestat] mean and std.dev: (473.21, 469.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3054) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692120 reads in 261.497 CPU sec, 9.470 real sec [M::mem_process_seqs] Processed 693298 reads in 176.030 CPU sec, 6.266 real sec [M::process] read 692696 sequences (100000160 bp) in 3.137 CPU sec, 3.444 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 284719, 17, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 389, 764) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1898) [M::mem_pestat] mean and std.dev: (481.55, 414.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 255, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (278.89, 137.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (509, 1507, 3592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9758) [M::mem_pestat] mean and std.dev: (1986.94, 1939.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12841) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 251, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (246.38, 124.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 10, #reads = 1083011 mem = 536879448 peak = 848711680 [M::process] read 693502 sequences (100000103 bp) in 3.887 CPU sec, 4.367 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 284021, 22, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 234, 1500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4230) [M::mem_pestat] mean and std.dev: (628.59, 769.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 260, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (282.11, 137.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (323, 858, 1357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3425) [M::mem_pestat] mean and std.dev: (925.24, 792.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4459) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 468, 787) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1905) [M::mem_pestat] mean and std.dev: (430.82, 269.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2464) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693502 reads in 262.025 CPU sec, 9.032 real sec [M::mem_process_seqs] Processed 692696 reads in 176.691 CPU sec, 6.047 real sec [M::process] read 693816 sequences (100000033 bp) in 3.602 CPU sec, 4.016 real sec... sorting block 11, #reads = 1082551 mem = 536878649 peak = 848711680 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 284415, 19, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 285, 642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1578) [M::mem_pestat] mean and std.dev: (360.50, 299.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2046) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 256, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (279.07, 136.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (471, 479, 2676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7086) [M::mem_pestat] mean and std.dev: (1145.39, 1355.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9291) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (249, 310, 687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1563) [M::mem_pestat] mean and std.dev: (397.11, 310.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2001) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693502 reads in 145.821 CPU sec, 5.030 real sec [M::process] read 696030 sequences (100000038 bp) in 3.496 CPU sec, 3.843 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 284295, 20, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (248, 302, 686) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1562) [M::mem_pestat] mean and std.dev: (343.71, 210.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2000) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 256, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (279.23, 137.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (7, 359, 956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2854) [M::mem_pestat] mean and std.dev: (459.68, 559.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3803) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 354, 819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2141) [M::mem_pestat] mean and std.dev: (510.59, 514.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2802) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 12, #reads = 1081300 mem = 536880984 peak = 848711680 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 285408, 18, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 258, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1171) [M::mem_pestat] mean and std.dev: (294.19, 226.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 253, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (275.58, 135.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (113, 221, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1178) [M::mem_pestat] mean and std.dev: (241.62, 176.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1533) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 413, 566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1250) [M::mem_pestat] mean and std.dev: (378.73, 216.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1592) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693816 reads in 259.200 CPU sec, 8.447 real sec [M::process] read 695728 sequences (100000250 bp) in 4.587 CPU sec, 5.062 real sec... [M::mem_process_seqs] Processed 696030 reads in 173.493 CPU sec, 5.616 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 285257, 12, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 324, 550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1290) [M::mem_pestat] mean and std.dev: (370.22, 281.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 254, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (275.51, 135.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (503, 827, 2245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5729) [M::mem_pestat] mean and std.dev: (1311.08, 1204.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7471) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 297, 721) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1795) [M::mem_pestat] mean and std.dev: (434.25, 403.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2332) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 13, #reads = 1081683 mem = 536879835 peak = 848711680 [M::process] read 693548 sequences (100000001 bp) in 3.795 CPU sec, 4.233 real sec... [M::mem_process_seqs] Processed 695728 reads in 127.328 CPU sec, 4.257 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 284775, 28, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 243, 544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1298) [M::mem_pestat] mean and std.dev: (344.67, 261.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 264, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (286.08, 141.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 997) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (347, 1329, 3145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8741) [M::mem_pestat] mean and std.dev: (2113.89, 2424.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11813) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 305, 710) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1814) [M::mem_pestat] mean and std.dev: (434.67, 434.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2366) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695160 sequences (100000249 bp) in 3.114 CPU sec, 3.231 real sec... sorting block 14, #reads = 1082367 mem = 536879784 peak = 848711680 [M::process] read 694636 sequences (100000191 bp) in 2.842 CPU sec, 2.987 real sec... [M::process] read 693072 sequences (100000056 bp) in 2.173 CPU sec, 2.182 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 285179, 13, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 257, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (246.06, 129.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 256, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (278.48, 137.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (197, 525, 1304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3518) [M::mem_pestat] mean and std.dev: (622.45, 495.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4625) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 275, 576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1410) [M::mem_pestat] mean and std.dev: (308.81, 236.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1827) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694636 reads in 109.595 CPU sec, 3.727 real sec sorting block 15, #reads = 1082514 mem = 536879102 peak = 848711680 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 285474, 17, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (242, 300, 609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1343) [M::mem_pestat] mean and std.dev: (358.57, 238.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 255, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (278.16, 137.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (517, 1141, 3495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9451) [M::mem_pestat] mean and std.dev: (1897.06, 2031.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12429) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 413, 748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1764) [M::mem_pestat] mean and std.dev: (530.25, 405.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2272) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695160 reads in 228.547 CPU sec, 8.463 real sec [M::process] read 692954 sequences (100000249 bp) in 3.339 CPU sec, 3.592 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 284412, 30, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 285, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (269.98, 99.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 262, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (284.22, 139.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (96, 684, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7617) [M::mem_pestat] mean and std.dev: (811.56, 1142.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10124) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 324, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1131) [M::mem_pestat] mean and std.dev: (285.48, 148.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1456) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693548 reads in 444.731 CPU sec, 15.557 real sec [M::mem_process_seqs] Processed 693072 reads in 147.627 CPU sec, 4.833 real sec sorting block 16, #reads = 1083073 mem = 536885120 peak = 848711680 [M::process] read 693704 sequences (100000143 bp) in 2.964 CPU sec, 3.156 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 284193, 19, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 266, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (255.65, 136.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 261, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (284.06, 139.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (673, 764, 2845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7189) [M::mem_pestat] mean and std.dev: (1323.22, 1312.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9361) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 354, 566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1348) [M::mem_pestat] mean and std.dev: (399.92, 262.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1739) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 17, #reads = 1080900 mem = 536879431 peak = 904069120 [M::process] read 693880 sequences (100000276 bp) in 2.470 CPU sec, 2.516 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 284375, 16, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 358, 898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2266) [M::mem_pestat] mean and std.dev: (509.09, 453.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2950) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 257, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (280.37, 137.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (145, 456, 1857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5281) [M::mem_pestat] mean and std.dev: (950.62, 1090.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6993) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 356, 976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2496) [M::mem_pestat] mean and std.dev: (573.72, 505.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3256) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692954 reads in 200.623 CPU sec, 6.402 real sec [M::process] read 693390 sequences (100000014 bp) in 2.183 CPU sec, 2.267 real sec... [M::mem_process_seqs] Processed 693704 reads in 136.278 CPU sec, 4.356 real sec sorting block 18, #reads = 1082527 mem = 536879480 peak = 904069120 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 283973, 22, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 232, 587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1437) [M::mem_pestat] mean and std.dev: (297.51, 189.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1862) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 255, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (278.00, 136.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (280, 560, 727) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1621) [M::mem_pestat] mean and std.dev: (492.25, 300.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2068) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 216, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1279) [M::mem_pestat] mean and std.dev: (334.34, 249.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1652) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693612 sequences (100000265 bp) in 3.049 CPU sec, 3.174 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 284102, 15, 84) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 323, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975) [M::mem_pestat] mean and std.dev: (325.76, 177.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1223) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 255, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (278.29, 136.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (68, 1183, 1363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3953) [M::mem_pestat] mean and std.dev: (666.54, 578.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5248) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 277, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024) [M::mem_pestat] mean and std.dev: (275.63, 150.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1298) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693880 reads in 182.649 CPU sec, 5.859 real sec [M::process] read 694320 sequences (100000040 bp) in 2.038 CPU sec, 2.054 real sec... [M::mem_process_seqs] Processed 693390 reads in 122.350 CPU sec, 3.864 real sec sorting block 19, #reads = 1082283 mem = 536880585 peak = 904069120 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 284500, 12, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 287, 631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1527) [M::mem_pestat] mean and std.dev: (315.27, 205.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1975) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 256, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (279.08, 137.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (225, 468, 4966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14448) [M::mem_pestat] mean and std.dev: (1598.75, 2152.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19189) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 329, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1145) [M::mem_pestat] mean and std.dev: (346.30, 207.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1466) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692948 sequences (100000063 bp) in 2.713 CPU sec, 2.810 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 283842, 22, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 287, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1263) [M::mem_pestat] mean and std.dev: (355.54, 266.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 257, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (278.13, 135.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (282, 592, 646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1374) [M::mem_pestat] mean and std.dev: (447.84, 228.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1738) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 283, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027) [M::mem_pestat] mean and std.dev: (303.58, 147.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1294) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693462 sequences (100000042 bp) in 1.820 CPU sec, 1.823 real sec... [M::mem_process_seqs] Processed 693612 reads in 174.855 CPU sec, 5.568 real sec [M::mem_process_seqs] Processed 694320 reads in 116.140 CPU sec, 3.696 real sec sorting block 20, #reads = 1082104 mem = 536879038 peak = 904069120 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 284078, 14, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (234, 656, 1045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2667) [M::mem_pestat] mean and std.dev: (585.60, 409.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 262, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (282.56, 136.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (953, 1926, 4404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11306) [M::mem_pestat] mean and std.dev: (2411.93, 1613.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14757) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (256, 405, 886) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2146) [M::mem_pestat] mean and std.dev: (459.93, 330.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2776) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693578 sequences (100000278 bp) in 3.076 CPU sec, 3.153 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 284545, 25, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (296, 314, 551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1061) [M::mem_pestat] mean and std.dev: (382.12, 258.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 261, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (282.09, 137.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (165, 339, 597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1461) [M::mem_pestat] mean and std.dev: (311.18, 164.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1893) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 243, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985) [M::mem_pestat] mean and std.dev: (269.71, 202.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1264) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692948 reads in 165.225 CPU sec, 5.261 real sec sorting block 21, #reads = 1081787 mem = 536876895 peak = 904069120 [M::mem_process_seqs] Processed 693462 reads in 110.024 CPU sec, 3.466 real sec [M::process] read 695532 sequences (100000094 bp) in 2.045 CPU sec, 2.111 real sec... sorting block 22, #reads = 1083285 mem = 536877614 peak = 989601792 [M::process] read 694726 sequences (100000157 bp) in 2.469 CPU sec, 2.550 real sec... [M::process] read 692954 sequences (100000213 bp) in 1.580 CPU sec, 1.577 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 285268, 23, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 262, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (286.30, 161.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 997) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 254, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (276.74, 136.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (344, 1055, 1503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3821) [M::mem_pestat] mean and std.dev: (915.25, 746.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4980) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 375, 669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1535) [M::mem_pestat] mean and std.dev: (447.46, 341.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1968) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695532 reads in 98.148 CPU sec, 3.104 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 284432, 25, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 279, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1177) [M::mem_pestat] mean and std.dev: (288.12, 230.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 262, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (284.03, 139.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 984) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (231, 697, 2671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7551) [M::mem_pestat] mean and std.dev: (1211.04, 1091.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9991) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 302, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1094) [M::mem_pestat] mean and std.dev: (291.76, 139.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1382) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 285108, 20, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 366, 656) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1612) [M::mem_pestat] mean and std.dev: (348.68, 258.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2090) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 255, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (278.00, 137.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (684, 1153, 2279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5469) [M::mem_pestat] mean and std.dev: (1428.11, 1239.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7064) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 381, 626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1490) [M::mem_pestat] mean and std.dev: (417.44, 337.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1922) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693494 sequences (100000152 bp) in 1.904 CPU sec, 1.909 real sec... [M::mem_process_seqs] Processed 693578 reads in 259.298 CPU sec, 8.245 real sec sorting block 23, #reads = 1082200 mem = 536877790 peak = 989601792 [M::mem_process_seqs] Processed 694726 reads in 127.421 CPU sec, 4.067 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 284699, 11, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 299, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961) [M::mem_pestat] mean and std.dev: (284.32, 151.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1227) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 262, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (284.92, 139.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (82, 456, 916) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2584) [M::mem_pestat] mean and std.dev: (474.40, 468.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3418) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 262, 662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1606) [M::mem_pestat] mean and std.dev: (398.88, 329.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2078) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 24, #reads = 1083302 mem = 536882858 peak = 1026621440 [M::process] read 693628 sequences (100000119 bp) in 2.855 CPU sec, 2.966 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 284461, 19, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 243, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (279.81, 165.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1143) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 259, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (282.12, 138.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (186, 218, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (268.00, 146.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 380, 639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1529) [M::mem_pestat] mean and std.dev: (378.64, 260.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1974) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692954 reads in 163.942 CPU sec, 5.193 real sec [M::mem_process_seqs] Processed 693494 reads in 112.792 CPU sec, 3.552 real sec [M::process] read 693620 sequences (100000130 bp) in 2.522 CPU sec, 2.546 real sec... sorting block 25, #reads = 1081991 mem = 536884794 peak = 1026621440 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 283976, 19, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 297, 783) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1987) [M::mem_pestat] mean and std.dev: (396.45, 330.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 256, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (279.25, 137.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (295, 391, 949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2257) [M::mem_pestat] mean and std.dev: (480.29, 281.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2911) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 288, 587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1409) [M::mem_pestat] mean and std.dev: (351.81, 271.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1820) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693346 sequences (100000237 bp) in 2.962 CPU sec, 3.143 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 284226, 22, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 247, 952) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2524) [M::mem_pestat] mean and std.dev: (604.85, 673.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3310) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 255, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (277.99, 136.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (338, 1221, 2271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6137) [M::mem_pestat] mean and std.dev: (1110.50, 849.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8070) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 316, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1246) [M::mem_pestat] mean and std.dev: (310.15, 171.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1600) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693628 reads in 162.175 CPU sec, 5.147 real sec [M::process] read 697398 sequences (100000266 bp) in 1.807 CPU sec, 1.802 real sec... [M::mem_process_seqs] Processed 693620 reads in 106.959 CPU sec, 3.386 real sec sorting block 26, #reads = 1080833 mem = 536881194 peak = 1026621440 [M::process] read 698296 sequences (100000116 bp) in 2.571 CPU sec, 2.650 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 285622, 24, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 369, 591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1443) [M::mem_pestat] mean and std.dev: (352.18, 251.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1869) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (270.47, 136.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (674, 1769, 5910) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16382) [M::mem_pestat] mean and std.dev: (2940.79, 3033.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21618) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 329, 579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1325) [M::mem_pestat] mean and std.dev: (354.75, 228.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1698) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698830 sequences (100000202 bp) in 1.610 CPU sec, 1.615 real sec... [M::mem_process_seqs] Processed 697398 reads in 91.530 CPU sec, 2.891 real sec sorting block 27, #reads = 1082018 mem = 536878110 peak = 1026621440 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 284089, 28, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 338, 646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1464) [M::mem_pestat] mean and std.dev: (351.00, 207.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1873) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 256, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (279.01, 136.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (440, 705, 1999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5117) [M::mem_pestat] mean and std.dev: (1083.46, 909.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6676) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 332, 659) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1569) [M::mem_pestat] mean and std.dev: (358.35, 227.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2024) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 286201, 16, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 234, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1084) [M::mem_pestat] mean and std.dev: (314.67, 226.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 244, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (272.14, 139.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (430, 816, 4614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12982) [M::mem_pestat] mean and std.dev: (1773.88, 1989.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17166) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (312, 715, 1454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3738) [M::mem_pestat] mean and std.dev: (889.35, 839.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4880) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693346 reads in 253.633 CPU sec, 8.034 real sec [M::process] read 696768 sequences (100000054 bp) in 2.365 CPU sec, 2.372 real sec... [M::mem_process_seqs] Processed 698296 reads in 128.555 CPU sec, 4.087 real sec sorting block 28, #reads = 1080683 mem = 536878177 peak = 1026621440 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 286545, 13, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 263, 702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1768) [M::mem_pestat] mean and std.dev: (432.17, 404.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2301) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 240, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (267.35, 135.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (539, 1034, 1993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4901) [M::mem_pestat] mean and std.dev: (1172.54, 787.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6355) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 378, 663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1541) [M::mem_pestat] mean and std.dev: (382.98, 217.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1980) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697892 sequences (100000206 bp) in 3.336 CPU sec, 3.469 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 285913, 10, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 378, 843) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2143) [M::mem_pestat] mean and std.dev: (382.18, 274.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (275.77, 139.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (140, 308, 1769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5027) [M::mem_pestat] mean and std.dev: (919.30, 1212.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6656) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 264, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999) [M::mem_pestat] mean and std.dev: (253.70, 155.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1274) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698830 reads in 163.024 CPU sec, 5.160 real sec [M::mem_process_seqs] Processed 696768 reads in 109.537 CPU sec, 3.449 real sec sorting block 29, #reads = 1082824 mem = 536880626 peak = 1026621440 [M::process] read 694892 sequences (100000152 bp) in 2.578 CPU sec, 2.639 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 286344, 16, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 217, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (230.11, 101.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 243, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (269.73, 136.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (224, 988, 3387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9713) [M::mem_pestat] mean and std.dev: (2454.31, 3531.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16579) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (281, 409, 906) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2156) [M::mem_pestat] mean and std.dev: (460.11, 338.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2781) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694694 sequences (100000136 bp) in 2.444 CPU sec, 2.514 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 285445, 31, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 220, 777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2093) [M::mem_pestat] mean and std.dev: (429.54, 469.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2751) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 255, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (280.65, 141.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (322, 726, 1573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4075) [M::mem_pestat] mean and std.dev: (870.90, 636.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5326) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (262, 526, 1407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3697) [M::mem_pestat] mean and std.dev: (717.60, 736.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4842) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697892 reads in 168.676 CPU sec, 5.349 real sec sorting block 30, #reads = 1081656 mem = 536879302 peak = 1026621440 [M::process] read 693350 sequences (100000160 bp) in 2.395 CPU sec, 2.395 real sec... [M::mem_process_seqs] Processed 694892 reads in 111.093 CPU sec, 3.497 real sec sorting block 31, #reads = 1080546 mem = 536878740 peak = 1092104192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 285019, 23, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 296, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1120) [M::mem_pestat] mean and std.dev: (306.85, 180.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 255, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (279.62, 139.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (498, 567, 1769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4311) [M::mem_pestat] mean and std.dev: (1088.52, 1116.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5582) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 295, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (279.72, 130.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1025) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693590 sequences (100000266 bp) in 2.622 CPU sec, 2.703 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 284751, 21, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 299, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1161) [M::mem_pestat] mean and std.dev: (312.80, 206.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 259, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (283.01, 140.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (152, 264, 649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1643) [M::mem_pestat] mean and std.dev: (376.85, 339.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2140) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 275, 639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1567) [M::mem_pestat] mean and std.dev: (333.38, 234.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2031) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693614 sequences (100000091 bp) in 1.610 CPU sec, 1.612 real sec... [M::mem_process_seqs] Processed 694694 reads in 169.121 CPU sec, 5.376 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 284693, 19, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 205, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1231) [M::mem_pestat] mean and std.dev: (271.77, 189.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 254, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (279.26, 137.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (782, 1798, 5863) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16025) [M::mem_pestat] mean and std.dev: (2569.79, 2245.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21106) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 340, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1173) [M::mem_pestat] mean and std.dev: (337.78, 176.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1494) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693590 reads in 90.864 CPU sec, 3.031 real sec [M::process] read 698822 sequences (100000126 bp) in 2.799 CPU sec, 2.805 real sec... sorting block 32, #reads = 1079199 mem = 536878661 peak = 1092104192 [M::mem_process_seqs] Processed 693350 reads in 226.458 CPU sec, 7.341 real sec sorting block 33, #reads = 1078867 mem = 536879353 peak = 1096028160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 284669, 14, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 263, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (231.06, 93.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 256, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (280.17, 138.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (487, 1042, 1985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4981) [M::mem_pestat] mean and std.dev: (1115.36, 859.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6479) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 279, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1148) [M::mem_pestat] mean and std.dev: (282.19, 154.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1467) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700526 sequences (100000222 bp) in 2.487 CPU sec, 2.560 real sec... [M::process] read 699108 sequences (100000089 bp) in 1.680 CPU sec, 1.675 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 286637, 11, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 442, 772) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1942) [M::mem_pestat] mean and std.dev: (558.95, 473.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 241, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (268.11, 135.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (377, 532, 841) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1769) [M::mem_pestat] mean and std.dev: (537.00, 323.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2233) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 278, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1192) [M::mem_pestat] mean and std.dev: (287.89, 182.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1528) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693614 reads in 196.018 CPU sec, 6.201 real sec [M::mem_process_seqs] Processed 698822 reads in 122.522 CPU sec, 3.891 real sec sorting block 34, #reads = 1077784 mem = 536878152 peak = 1096028160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 287526, 18, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 230, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060) [M::mem_pestat] mean and std.dev: (282.97, 172.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 237, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (262.81, 131.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (677, 1253, 2214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5288) [M::mem_pestat] mean and std.dev: (1490.78, 1034.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6825) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 256, 598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1396) [M::mem_pestat] mean and std.dev: (406.51, 271.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1795) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698190 sequences (100000274 bp) in 2.767 CPU sec, 2.933 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 287212, 10, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 222, 738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1864) [M::mem_pestat] mean and std.dev: (437.58, 459.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 241, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (265.53, 132.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (405, 837, 1466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3588) [M::mem_pestat] mean and std.dev: (1078.50, 931.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4804) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 235, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (259.41, 147.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1192) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697964 sequences (100000108 bp) in 2.320 CPU sec, 2.320 real sec... [M::mem_process_seqs] Processed 700526 reads in 186.684 CPU sec, 5.902 real sec [M::mem_process_seqs] Processed 699108 reads in 122.997 CPU sec, 3.891 real sec sorting block 35, #reads = 1075525 mem = 536882690 peak = 1096028160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 286260, 26, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 380, 1481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4081) [M::mem_pestat] mean and std.dev: (674.80, 674.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5381) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 243, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (268.39, 134.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (352, 688, 816) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1744) [M::mem_pestat] mean and std.dev: (523.90, 229.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2208) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 406, 766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1864) [M::mem_pestat] mean and std.dev: (478.09, 330.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2413) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695556 sequences (100000247 bp) in 2.559 CPU sec, 2.632 real sec... [M::process] read 694978 sequences (100000219 bp) in 1.670 CPU sec, 1.666 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 285896, 15, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 232, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (266.57, 121.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 243, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (268.64, 134.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (252, 633, 2146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5934) [M::mem_pestat] mean and std.dev: (1206.20, 1215.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7828) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 313, 558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1336) [M::mem_pestat] mean and std.dev: (382.39, 259.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1725) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698190 reads in 176.070 CPU sec, 5.592 real sec sorting block 36, #reads = 1078828 mem = 536883004 peak = 1096028160 [M::mem_process_seqs] Processed 697964 reads in 115.990 CPU sec, 3.692 real sec sorting block 37, #reads = 1078814 mem = 536877933 peak = 1096028160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 285677, 30, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 225, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (236.53, 117.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 253, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (278.59, 139.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (234, 1068, 1994) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5514) [M::mem_pestat] mean and std.dev: (1287.00, 1280.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7274) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 286, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (269.30, 108.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693488 sequences (100000012 bp) in 2.450 CPU sec, 2.535 real sec... [M::process] read 694560 sequences (100000035 bp) in 1.690 CPU sec, 1.683 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 284956, 26, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 292, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (283.50, 141.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 253, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (278.70, 139.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (245, 798, 1346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3548) [M::mem_pestat] mean and std.dev: (799.26, 682.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4649) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 366, 698) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1714) [M::mem_pestat] mean and std.dev: (459.08, 395.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2222) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695556 reads in 173.506 CPU sec, 5.515 real sec [M::mem_process_seqs] Processed 694978 reads in 115.276 CPU sec, 3.665 real sec sorting block 38, #reads = 1079045 mem = 536877739 peak = 1096028160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 284437, 12, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 223, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (245.52, 144.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 258, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (283.48, 141.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (584, 634, 1046) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1970) [M::mem_pestat] mean and std.dev: (773.36, 296.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2432) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 286, 947) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2473) [M::mem_pestat] mean and std.dev: (467.81, 420.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3236) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694906 sequences (100000259 bp) in 2.576 CPU sec, 2.652 real sec... [M::process] read 699804 sequences (100000168 bp) in 1.680 CPU sec, 1.680 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 284358, 24, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 342, 845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2201) [M::mem_pestat] mean and std.dev: (471.38, 492.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2879) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 253, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (279.18, 140.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (301, 743, 1599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4195) [M::mem_pestat] mean and std.dev: (878.43, 768.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5493) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 284, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (298.38, 139.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1007) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693488 reads in 169.694 CPU sec, 5.383 real sec [M::mem_process_seqs] Processed 694560 reads in 111.421 CPU sec, 3.543 real sec sorting block 39, #reads = 1077580 mem = 536887027 peak = 1096028160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 284310, 24, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 395, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053) [M::mem_pestat] mean and std.dev: (318.52, 168.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1341) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 251, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (277.73, 139.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (141, 324, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1269) [M::mem_pestat] mean and std.dev: (266.90, 211.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1645) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 274, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (297.73, 154.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697970 sequences (100000092 bp) in 2.476 CPU sec, 2.549 real sec... [M::process] read 695904 sequences (100000232 bp) in 1.650 CPU sec, 1.644 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 286465, 22, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 218, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1161) [M::mem_pestat] mean and std.dev: (308.90, 268.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 240, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (265.63, 133.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (230, 1810, 3232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9236) [M::mem_pestat] mean and std.dev: (1720.68, 1564.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12238) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 310, 665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1549) [M::mem_pestat] mean and std.dev: (403.50, 276.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1991) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694906 reads in 174.521 CPU sec, 5.542 real sec sorting block 40, #reads = 1078722 mem = 536879901 peak = 1096028160 [M::mem_process_seqs] Processed 699804 reads in 111.335 CPU sec, 3.548 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 286301, 29, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 383, 1083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2807) [M::mem_pestat] mean and std.dev: (560.43, 599.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 246, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (271.36, 136.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (214, 357, 930) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2362) [M::mem_pestat] mean and std.dev: (501.00, 444.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3078) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 368, 667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1599) [M::mem_pestat] mean and std.dev: (433.17, 332.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2065) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 41, #reads = 1076230 mem = 536880575 peak = 1096028160 [M::process] read 698292 sequences (100000069 bp) in 2.667 CPU sec, 2.723 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 286052, 22, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 294, 664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1730) [M::mem_pestat] mean and std.dev: (369.02, 352.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2263) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 253, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (277.70, 138.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (260, 266, 1369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3587) [M::mem_pestat] mean and std.dev: (566.30, 567.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4696) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 321, 891) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2209) [M::mem_pestat] mean and std.dev: (527.24, 459.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2868) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697372 sequences (100000015 bp) in 1.700 CPU sec, 1.703 real sec... [M::mem_process_seqs] Processed 697970 reads in 157.763 CPU sec, 5.005 real sec [M::mem_process_seqs] Processed 695904 reads in 102.428 CPU sec, 3.241 real sec sorting block 42, #reads = 1082296 mem = 536879042 peak = 1096028160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 286164, 22, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 275, 1086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2874) [M::mem_pestat] mean and std.dev: (531.91, 536.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 243, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (266.64, 131.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (318, 457, 615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1209) [M::mem_pestat] mean and std.dev: (368.47, 181.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1506) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (244, 386, 850) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2062) [M::mem_pestat] mean and std.dev: (469.24, 357.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2668) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695410 sequences (100000000 bp) in 2.447 CPU sec, 2.513 real sec... [M::process] read 696144 sequences (100000147 bp) in 1.700 CPU sec, 1.699 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 285673, 20, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 249, 821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2163) [M::mem_pestat] mean and std.dev: (416.58, 482.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2834) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (270.02, 133.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (531, 748, 1881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4581) [M::mem_pestat] mean and std.dev: (991.74, 782.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5931) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 367, 975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2515) [M::mem_pestat] mean and std.dev: (387.94, 336.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3285) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698292 reads in 161.310 CPU sec, 5.129 real sec [M::mem_process_seqs] Processed 697372 reads in 112.162 CPU sec, 3.578 real sec sorting block 43, #reads = 1082268 mem = 536880132 peak = 1096028160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 286004, 16, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 222, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (219.33, 101.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 253, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (277.40, 137.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (332, 569, 1808) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4760) [M::mem_pestat] mean and std.dev: (878.31, 719.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6236) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 251, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (265.67, 128.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1025) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695722 sequences (100000018 bp) in 2.418 CPU sec, 2.473 real sec... [M::process] read 695492 sequences (100000036 bp) in 1.660 CPU sec, 1.658 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 286101, 14, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 370, 936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2382) [M::mem_pestat] mean and std.dev: (584.20, 512.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3105) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (273.33, 135.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (153, 752, 1852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5250) [M::mem_pestat] mean and std.dev: (917.07, 904.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6949) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 254, 536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1234) [M::mem_pestat] mean and std.dev: (322.00, 240.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1583) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695410 reads in 160.640 CPU sec, 5.119 real sec [M::mem_process_seqs] Processed 696144 reads in 106.293 CPU sec, 3.387 real sec sorting block 44, #reads = 1081877 mem = 536877290 peak = 1096028160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 285122, 14, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 333, 677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1681) [M::mem_pestat] mean and std.dev: (376.24, 306.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2183) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 250, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (275.75, 137.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (589, 760, 1039) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1939) [M::mem_pestat] mean and std.dev: (742.33, 381.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2389) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 249, 888) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2254) [M::mem_pestat] mean and std.dev: (503.10, 531.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2937) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696096 sequences (100000012 bp) in 2.406 CPU sec, 2.463 real sec... [M::process] read 701512 sequences (100000232 bp) in 1.700 CPU sec, 1.700 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 285223, 19, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 223, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (253.22, 100.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 250, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (276.56, 138.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (398, 1007, 2052) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5360) [M::mem_pestat] mean and std.dev: (1154.32, 912.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7014) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 250, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940) [M::mem_pestat] mean and std.dev: (298.94, 159.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1191) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695722 reads in 166.639 CPU sec, 5.292 real sec sorting block 45, #reads = 1081301 mem = 536877202 peak = 1096028160 [M::mem_process_seqs] Processed 695492 reads in 109.816 CPU sec, 3.487 real sec sorting block 46, #reads = 1078784 mem = 536884598 peak = 1116942336 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 285236, 18, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 204, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (225.73, 119.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 246, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (271.77, 135.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (122, 875, 1363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3845) [M::mem_pestat] mean and std.dev: (883.00, 740.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5086) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 300, 555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1279) [M::mem_pestat] mean and std.dev: (384.90, 312.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1641) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700560 sequences (100000170 bp) in 2.558 CPU sec, 2.606 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 287263, 19, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 253, 649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1531) [M::mem_pestat] mean and std.dev: (351.06, 259.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1972) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 234, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (261.05, 131.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (82, 345, 846) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2374) [M::mem_pestat] mean and std.dev: (464.76, 535.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3138) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 333, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1169) [M::mem_pestat] mean and std.dev: (347.83, 243.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1502) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699704 sequences (100000295 bp) in 2.320 CPU sec, 2.318 real sec... [M::mem_process_seqs] Processed 696096 reads in 182.425 CPU sec, 5.781 real sec [M::process] read 700554 sequences (100000299 bp) in 2.077 CPU sec, 2.086 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 287361, 8, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 227, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1259) [M::mem_pestat] mean and std.dev: (303.39, 242.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 236, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (261.97, 130.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 376, 605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1391) [M::mem_pestat] mean and std.dev: (327.46, 159.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1784) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700560 reads in 98.967 CPU sec, 3.131 real sec sorting block 47, #reads = 1075334 mem = 536879872 peak = 1116942336 [M::mem_process_seqs] Processed 701512 reads in 243.532 CPU sec, 7.720 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 286997, 15, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 224, 762) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1916) [M::mem_pestat] mean and std.dev: (481.00, 522.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 240, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (266.98, 134.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (719, 1231, 2738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6776) [M::mem_pestat] mean and std.dev: (1556.00, 1245.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8795) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 334, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (267.33, 115.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 48, #reads = 1076172 mem = 536880135 peak = 1116942336 [M::process] read 697816 sequences (100000250 bp) in 3.129 CPU sec, 3.220 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 287368, 24, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 197, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (263.33, 200.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 234, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (259.46, 128.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (420, 1087, 3416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9408) [M::mem_pestat] mean and std.dev: (1629.00, 1396.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12404) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 281, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (251.22, 88.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699704 reads in 195.686 CPU sec, 6.213 real sec [M::mem_process_seqs] Processed 700554 reads in 117.007 CPU sec, 3.686 real sec [M::process] read 697752 sequences (100000160 bp) in 2.335 CPU sec, 2.383 real sec... sorting block 49, #reads = 1078580 mem = 536878816 peak = 1116942336 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 286122, 11, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 257, 628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1534) [M::mem_pestat] mean and std.dev: (312.50, 200.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1987) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (269.60, 133.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (191, 277, 1807) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5039) [M::mem_pestat] mean and std.dev: (737.70, 820.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6655) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 317, 1094) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2874) [M::mem_pestat] mean and std.dev: (701.09, 731.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3764) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696586 sequences (100000129 bp) in 2.579 CPU sec, 2.698 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 286325, 25, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 327, 549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1301) [M::mem_pestat] mean and std.dev: (379.91, 268.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (268.80, 132.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (446, 1443, 1570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3818) [M::mem_pestat] mean and std.dev: (1102.45, 849.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4942) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 347, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (317.31, 130.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695840 sequences (100000060 bp) in 1.630 CPU sec, 1.627 real sec... [M::mem_process_seqs] Processed 697816 reads in 170.975 CPU sec, 5.445 real sec [M::mem_process_seqs] Processed 697752 reads in 119.762 CPU sec, 3.821 real sec sorting block 50, #reads = 1079666 mem = 536877404 peak = 1116942336 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 285348, 20, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 273, 629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1561) [M::mem_pestat] mean and std.dev: (389.42, 339.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2027) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (271.77, 134.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (92, 1277, 1859) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5393) [M::mem_pestat] mean and std.dev: (983.68, 750.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7160) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 350, 956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2492) [M::mem_pestat] mean and std.dev: (479.71, 389.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3260) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695136 sequences (100000208 bp) in 2.544 CPU sec, 2.615 real sec... [M::process] read 421780 sequences (60641474 bp) in 1.100 CPU sec, 1.098 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 285209, 19, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 268, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (258.63, 153.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (273.32, 135.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1414, 1479, 2436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4480) [M::mem_pestat] mean and std.dev: (1514.81, 672.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5502) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 228, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (243.34, 98.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696586 reads in 167.317 CPU sec, 5.331 real sec sorting block 51, #reads = 1080694 mem = 536882692 peak = 1116942336 [M::mem_process_seqs] Processed 695840 reads in 115.654 CPU sec, 3.696 real sec sorting block 52, #reads = 1079628 mem = 536881700 peak = 1126567936 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 284455, 16, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 311, 951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2455) [M::mem_pestat] mean and std.dev: (444.95, 387.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3207) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 250, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (275.14, 136.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (302, 763, 1405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3611) [M::mem_pestat] mean and std.dev: (719.36, 626.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4714) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 362, 577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1313) [M::mem_pestat] mean and std.dev: (381.48, 257.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1681) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 172911, 23, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 538, 1024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2696) [M::mem_pestat] mean and std.dev: (499.55, 451.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 248, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (273.49, 136.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (347, 1030, 2202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5912) [M::mem_pestat] mean and std.dev: (1277.00, 1162.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7767) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 303, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1096) [M::mem_pestat] mean and std.dev: (265.40, 140.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1406) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695136 reads in 135.685 CPU sec, 4.401 real sec [M::mem_process_seqs] Processed 421780 reads in 72.567 CPU sec, 2.353 real sec sorting block 53, #reads = 1078501 mem = 536879785 peak = 1126567936 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000606a6 features: 1f8bfbff fffa320b extended: f1bf07ab 00407f5a amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8375C CPU @ 2.90GHz threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 58132110 overall: 69933047808 mem 10952.375 user 566.190 sys 432.276 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_5TB-T1-TDNA-1_A23MHWWLT4_1.L008\tPU:L008\tSM:1136_5TB-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_5TB-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0003.1136_5TB-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0003.1136_5TB-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 432.308 sec; CPU: 11518.568 sec sorting block 54, #reads = 143620 mem = 71562546 peak = 1126567936 uniq header count 1 merging 55 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000606a6 features: 1f8bfbff fffa320b extended: f1bf07ab 00407f5a amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8375C CPU @ 2.90GHz threads: 31 max 128 algo: util-sort license: sentieon:util=1 output file size: 3338972812 output reads: 58509879 bam_mem_sort: 55 calls 55.897 user 0.914 sys 57.536 real bam_write: 55 calls 24.133 user 1.954 sys 37.553 real execute: 1 calls 17.288 user 0.558 sys 453.950 real merge_files: 1 calls 17.229 user 0.546 sys 21.473 real parse_chunk: 867 calls 55.753 user 11.996 sys 72.485 real read_chunk: 2343 calls 26.012 user 18.106 sys 321.807 real sort_block: 1 calls 20.102 user 16.804 sys 312.212 real write_chunk: 1474 calls 16.751 user 0.351 sys 20.500 real overall: 2789404672 mem 893.083 user 45.029 sys 453.973 real ls: cannot access '*.cram': No such file or directory