.command.err - Workdir - DAQ:SENTIEON_BWAMEM (1029_N5D-T1-TDNA-1_A23MHWWLT4_1;L007;0003)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ab/1dbf7f01622ba31c98707f6a8c9f71/.command.err
Size: 138.2 KB
Task: DAQ:SENTIEON_BWAMEM (1029_N5D-T1-TDNA-1_A23MHWWLT4_1;L007;0003)
Attempt
Content

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1029_N5D-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1667.554 CPU sec, 774.749 real sec
[M::process] read 685482 sequences (100000210 bp) in 0.950 CPU sec, 0.958 real sec...
[M::process] read 684118 sequences (100000170 bp) in 1.073 CPU sec, 1.081 real sec...
[M::process] read 682994 sequences (100000237 bp) in 1.146 CPU sec, 1.171 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 279375, 3, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 256, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (261.66, 131.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 322, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (339.70, 165.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 221, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (270.82, 131.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682172 sequences (100000130 bp) in 1.152 CPU sec, 1.172 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 278473, 7, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 263, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (267.66, 131.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1080)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 331, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (346.30, 166.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 232, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (289.93, 141.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 278018, 7, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 330, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (312.41, 180.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 335, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (350.14, 166.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (298, 345, 583)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1153)
[M::mem_pestat] mean and std.dev: (390.06, 199.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1438)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685482 reads in 221.265 CPU sec, 7.094 real sec
[M::mem_process_seqs] Processed 684118 reads in 164.963 CPU sec, 5.333 real sec
[M::mem_process_seqs] Processed 682994 reads in 180.382 CPU sec, 5.845 real sec
sorting block 0, #reads = 1072897 mem = 536879772 peak = 605495296
[M::process] read 681790 sequences (100000247 bp) in 1.879 CPU sec, 2.123 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 277531, 2, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 316, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1092)
[M::mem_pestat] mean and std.dev: (317.04, 124.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1394)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 343, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (356.89, 169.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 280, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (289.62, 133.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681938 sequences (100000006 bp) in 1.379 CPU sec, 1.435 real sec...
[M::process] read 681464 sequences (100000105 bp) in 1.160 CPU sec, 1.161 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 277751, 6, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 251, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (322.39, 197.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 342, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (356.47, 168.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 360, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (348.32, 171.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682172 reads in 159.215 CPU sec, 5.120 real sec
sorting block 1, #reads = 1070442 mem = 536878827 peak = 732897280
[M::mem_process_seqs] Processed 681790 reads in 102.832 CPU sec, 3.286 real sec
sorting block 2, #reads = 1069052 mem = 536888653 peak = 873345024
[M::process] read 681280 sequences (100000104 bp) in 1.571 CPU sec, 1.673 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 278115, 1, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 240, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (237.54, 112.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 341, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (356.65, 169.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 270, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (259.32, 92.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 737)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681378 sequences (100000162 bp) in 1.291 CPU sec, 1.302 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 277503, 0, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 273, 1160)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3096)
[M::mem_pestat] mean and std.dev: (523.27, 495.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4064)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 343, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (357.44, 168.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 339, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (296.88, 105.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 884)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 277511, 10, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 286, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (272.27, 133.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 339, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (353.83, 165.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (393, 1726, 1726)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4392)
[M::mem_pestat] mean and std.dev: (1222.11, 707.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5725)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 263, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068)
[M::mem_pestat] mean and std.dev: (352.97, 222.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1352)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681938 reads in 228.667 CPU sec, 7.332 real sec
[M::mem_process_seqs] Processed 681464 reads in 171.898 CPU sec, 5.542 real sec
[M::mem_process_seqs] Processed 681280 reads in 187.066 CPU sec, 6.047 real sec
sorting block 3, #reads = 1069619 mem = 536881317 peak = 873345024
[M::process] read 681698 sequences (100000168 bp) in 1.925 CPU sec, 2.126 real sec...
sorting block 4, #reads = 1068841 mem = 536886103 peak = 873345024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 277006, 4, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (222, 302, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (270.05, 74.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 718)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 338, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (352.82, 165.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 287, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (263.44, 101.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681556 sequences (100000106 bp) in 1.507 CPU sec, 1.559 real sec...
[M::process] read 680930 sequences (100000101 bp) in 1.300 CPU sec, 1.302 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 276906, 9, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 341, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (306.61, 147.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 337, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (351.85, 165.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 236, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046)
[M::mem_pestat] mean and std.dev: (281.90, 188.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1335)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681378 reads in 166.968 CPU sec, 5.331 real sec
[M::mem_process_seqs] Processed 681698 reads in 106.015 CPU sec, 3.377 real sec
sorting block 5, #reads = 1067432 mem = 536880717 peak = 873345024
[M::process] read 684074 sequences (100000159 bp) in 1.803 CPU sec, 1.942 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 276890, 4, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 221, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886)
[M::mem_pestat] mean and std.dev: (245.45, 127.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 339, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (354.11, 167.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 322, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1122)
[M::mem_pestat] mean and std.dev: (358.63, 196.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1435)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683812 sequences (100000021 bp) in 1.318 CPU sec, 1.324 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278010, 4, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (205, 433, 615)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1435)
[M::mem_pestat] mean and std.dev: (343.92, 168.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1845)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 346, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (361.77, 171.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 296, 531)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1169)
[M::mem_pestat] mean and std.dev: (328.23, 206.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1488)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 278540, 5, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 220, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (260.94, 120.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 328, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (345.76, 168.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 247, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1155)
[M::mem_pestat] mean and std.dev: (302.33, 160.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1477)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681556 reads in 230.196 CPU sec, 7.398 real sec
[M::mem_process_seqs] Processed 680930 reads in 170.591 CPU sec, 5.502 real sec
sorting block 6, #reads = 1067585 mem = 536878123 peak = 873345024
[M::mem_process_seqs] Processed 684074 reads in 186.047 CPU sec, 6.027 real sec
[M::process] read 682892 sequences (100000301 bp) in 1.871 CPU sec, 2.048 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 278285, 5, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 248, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602)
[M::mem_pestat] mean and std.dev: (261.31, 108.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 745)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 328, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (343.62, 164.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 253, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (255.22, 92.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 7, #reads = 1067958 mem = 536880648 peak = 873345024
[M::process] read 682658 sequences (100000210 bp) in 1.670 CPU sec, 1.775 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 278354, 11, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 226, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483)
[M::mem_pestat] mean and std.dev: (230.55, 103.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 644)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 333, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (348.02, 165.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1045, 1236, 3275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7735)
[M::mem_pestat] mean and std.dev: (1794.82, 999.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9965)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (137, 263, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (260.70, 139.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683812 reads in 159.402 CPU sec, 5.098 real sec
[M::process] read 682410 sequences (100000287 bp) in 1.356 CPU sec, 1.361 real sec...
[M::mem_process_seqs] Processed 682892 reads in 101.957 CPU sec, 3.235 real sec
sorting block 8, #reads = 1072813 mem = 536878791 peak = 873345024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 278103, 7, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 221, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (254.65, 132.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 339, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (352.73, 167.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 279, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626)
[M::mem_pestat] mean and std.dev: (302.03, 121.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681710 sequences (100000278 bp) in 1.972 CPU sec, 2.131 real sec...
[M::process] read 681468 sequences (100000036 bp) in 1.400 CPU sec, 1.396 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 278041, 1, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 273, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (293.41, 131.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 341, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (354.78, 167.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 360, 629)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1477)
[M::mem_pestat] mean and std.dev: (303.93, 167.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1901)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682658 reads in 157.710 CPU sec, 5.041 real sec
[M::mem_process_seqs] Processed 682410 reads in 100.276 CPU sec, 3.202 real sec
sorting block 9, #reads = 1070820 mem = 536879936 peak = 873345024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 277686, 4, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (216, 246, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (277.95, 109.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 343, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (356.59, 167.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 266, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074)
[M::mem_pestat] mean and std.dev: (352.47, 202.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1360)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681442 sequences (100000034 bp) in 1.988 CPU sec, 2.121 real sec...
[M::process] read 681678 sequences (100000170 bp) in 1.420 CPU sec, 1.424 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 278222, 5, 50)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (214, 283, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (319.52, 203.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 341, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (355.40, 167.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 315, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (345.62, 153.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681710 reads in 158.733 CPU sec, 5.070 real sec
sorting block 10, #reads = 1072309 mem = 536876806 peak = 873345024
[M::mem_process_seqs] Processed 681468 reads in 100.307 CPU sec, 3.205 real sec
sorting block 11, #reads = 1069996 mem = 536884569 peak = 903520256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278376, 6, 50)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 272, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (309.12, 187.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 344, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (357.15, 167.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 293, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (326.43, 132.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1094)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681622 sequences (100000297 bp) in 2.223 CPU sec, 2.383 real sec...
[M::process] read 681540 sequences (100000192 bp) in 1.400 CPU sec, 1.402 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 277519, 8, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 295, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (289.32, 142.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 341, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (355.88, 168.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 249, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (239.44, 112.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681442 reads in 159.847 CPU sec, 5.102 real sec
[M::mem_process_seqs] Processed 681678 reads in 102.085 CPU sec, 3.259 real sec
sorting block 12, #reads = 1069680 mem = 536878918 peak = 903520256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 277696, 3, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 220, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491)
[M::mem_pestat] mean and std.dev: (226.60, 75.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 592)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 339, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (354.09, 166.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 242, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627)
[M::mem_pestat] mean and std.dev: (240.64, 104.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 777)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681338 sequences (100000285 bp) in 2.234 CPU sec, 2.407 real sec...
[M::process] read 682530 sequences (100000117 bp) in 1.460 CPU sec, 1.462 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 278356, 1, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 278, 577)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1355)
[M::mem_pestat] mean and std.dev: (363.41, 263.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1744)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 340, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (355.06, 167.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 291, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (295.65, 122.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681622 reads in 165.304 CPU sec, 5.281 real sec
[M::mem_process_seqs] Processed 681540 reads in 103.761 CPU sec, 3.312 real sec
sorting block 13, #reads = 1068869 mem = 536879663 peak = 903520256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 277945, 5, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 277, 696)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1748)
[M::mem_pestat] mean and std.dev: (460.17, 459.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2300)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 343, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (359.02, 170.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 313, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (324.39, 170.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684462 sequences (100000281 bp) in 2.238 CPU sec, 2.405 real sec...
[M::process] read 683098 sequences (100000285 bp) in 1.420 CPU sec, 1.426 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 278442, 7, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 223, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (277.04, 176.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 338, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (355.43, 171.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 396, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050)
[M::mem_pestat] mean and std.dev: (355.92, 176.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1335)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681338 reads in 160.215 CPU sec, 5.117 real sec
sorting block 14, #reads = 1068622 mem = 536883532 peak = 903520256
[M::mem_process_seqs] Processed 682530 reads in 100.937 CPU sec, 3.222 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 278987, 6, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 238, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (303.29, 191.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1296)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 326, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (342.84, 165.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (251.96, 135.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 852)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 15, #reads = 1068867 mem = 536882701 peak = 903520256
[M::process] read 682286 sequences (100000151 bp) in 2.266 CPU sec, 2.428 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 278856, 9, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (228, 319, 536)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1152)
[M::mem_pestat] mean and std.dev: (315.28, 161.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1460)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 333, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (347.98, 165.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 237, 936)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2386)
[M::mem_pestat] mean and std.dev: (621.54, 699.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3419)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682206 sequences (100000228 bp) in 1.690 CPU sec, 1.693 real sec...
[M::mem_process_seqs] Processed 684462 reads in 150.045 CPU sec, 4.770 real sec
[M::mem_process_seqs] Processed 683098 reads in 97.495 CPU sec, 3.093 real sec
sorting block 16, #reads = 1072234 mem = 536880481 peak = 903520256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 278555, 3, 58)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 171, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511)
[M::mem_pestat] mean and std.dev: (186.04, 83.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 636)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 338, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (353.09, 167.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 347, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (332.80, 160.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681370 sequences (100000074 bp) in 2.226 CPU sec, 2.380 real sec...
[M::process] read 680966 sequences (100000185 bp) in 1.400 CPU sec, 1.392 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 278749, 3, 50)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 339, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (290.18, 104.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 344, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (358.91, 171.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 206, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 449)
[M::mem_pestat] mean and std.dev: (208.43, 56.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 537)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682286 reads in 151.317 CPU sec, 4.811 real sec
[M::mem_process_seqs] Processed 682206 reads in 97.023 CPU sec, 3.087 real sec
sorting block 17, #reads = 1071359 mem = 536878739 peak = 903520256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 278100, 4, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 247, 543)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1275)
[M::mem_pestat] mean and std.dev: (285.82, 166.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1641)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 345, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (359.71, 170.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 267, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (306.41, 155.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 934)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681170 sequences (100000105 bp) in 2.144 CPU sec, 2.279 real sec...
[M::process] read 681262 sequences (100000005 bp) in 1.390 CPU sec, 1.395 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 277978, 5, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (199, 275, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1072)
[M::mem_pestat] mean and std.dev: (351.61, 235.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1363)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 347, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (361.23, 170.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 268, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (277.19, 121.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681370 reads in 153.554 CPU sec, 4.882 real sec
[M::mem_process_seqs] Processed 680966 reads in 98.117 CPU sec, 3.125 real sec
sorting block 18, #reads = 1069320 mem = 536879548 peak = 903520256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 278023, 7, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (214, 294, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (349.76, 183.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 343, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (358.15, 169.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 334, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (333.52, 135.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681360 sequences (100000004 bp) in 2.341 CPU sec, 2.496 real sec...
[M::process] read 681728 sequences (100000284 bp) in 1.370 CPU sec, 1.369 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 277631, 16, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 294, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (241.16, 121.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 340, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (356.23, 169.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (385, 1482, 1775)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4555)
[M::mem_pestat] mean and std.dev: (1062.80, 801.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5945)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 410, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (359.90, 149.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1105)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681170 reads in 154.235 CPU sec, 4.911 real sec
sorting block 19, #reads = 1069220 mem = 536885457 peak = 903520256
[M::mem_process_seqs] Processed 681262 reads in 99.689 CPU sec, 3.175 real sec
sorting block 20, #reads = 1069485 mem = 536877126 peak = 994086912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 277806, 6, 50)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 322, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (279.18, 113.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 336, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (352.97, 167.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 363, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (350.49, 140.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681306 sequences (100000246 bp) in 2.369 CPU sec, 2.515 real sec...
[M::process] read 680878 sequences (100000158 bp) in 1.410 CPU sec, 1.403 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 277929, 5, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 334, 934)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2390)
[M::mem_pestat] mean and std.dev: (477.10, 368.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3118)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 336, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (352.02, 166.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 253, 669)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1611)
[M::mem_pestat] mean and std.dev: (396.07, 396.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2082)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681360 reads in 154.139 CPU sec, 4.900 real sec
[M::mem_process_seqs] Processed 681728 reads in 99.167 CPU sec, 3.155 real sec
sorting block 21, #reads = 1069371 mem = 536878376 peak = 994086912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 278303, 7, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (201, 242, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (261.00, 128.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 341, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (357.80, 170.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 258, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (249.10, 110.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683512 sequences (100000127 bp) in 2.261 CPU sec, 2.391 real sec...
[M::process] read 683302 sequences (100000098 bp) in 1.360 CPU sec, 1.356 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 277820, 6, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (239, 343, 951)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2375)
[M::mem_pestat] mean and std.dev: (579.30, 641.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3146)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 349, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (365.34, 175.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 232, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (269.56, 119.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681306 reads in 152.332 CPU sec, 4.845 real sec
[M::mem_process_seqs] Processed 680878 reads in 98.392 CPU sec, 3.131 real sec
sorting block 22, #reads = 1068552 mem = 536885092 peak = 994086912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 278532, 3, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 269, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (278.60, 137.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 333, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (350.65, 170.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 280, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (280.08, 160.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682396 sequences (100000288 bp) in 2.143 CPU sec, 2.280 real sec...
[M::process] read 681876 sequences (100000057 bp) in 1.390 CPU sec, 1.385 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 278455, 10, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 337, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (271.36, 148.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 334, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (351.49, 170.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (382, 736, 1489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3703)
[M::mem_pestat] mean and std.dev: (820.30, 495.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4810)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 318, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (327.78, 136.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683512 reads in 153.216 CPU sec, 4.873 real sec
sorting block 23, #reads = 1068273 mem = 536878064 peak = 994086912
[M::mem_process_seqs] Processed 683302 reads in 97.834 CPU sec, 3.116 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 278084, 10, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 263, 577)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1477)
[M::mem_pestat] mean and std.dev: (345.94, 276.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1927)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 337, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (353.59, 169.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (415, 2127, 2179)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5707)
[M::mem_pestat] mean and std.dev: (1355.00, 853.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7471)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 251, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (306.79, 155.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 24, #reads = 1073410 mem = 536878881 peak = 994086912
[M::process] read 681568 sequences (100000160 bp) in 2.359 CPU sec, 2.497 real sec...
[M::process] read 681542 sequences (100000262 bp) in 1.360 CPU sec, 1.356 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 278162, 9, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 201, 581)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1481)
[M::mem_pestat] mean and std.dev: (258.00, 170.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1931)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 345, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (360.70, 172.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 325, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (318.38, 153.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1087)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682396 reads in 153.058 CPU sec, 4.869 real sec
[M::mem_process_seqs] Processed 681876 reads in 98.414 CPU sec, 3.129 real sec
sorting block 25, #reads = 1071113 mem = 536883691 peak = 994086912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 277602, 4, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 303, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (271.71, 111.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 347, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (362.62, 173.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 248, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (252.17, 100.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680626 sequences (100000253 bp) in 2.742 CPU sec, 2.890 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 277294, 5, 51)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 223, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (218.39, 85.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 347, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (361.36, 171.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 341, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (306.54, 137.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681756 sequences (100000241 bp) in 1.540 CPU sec, 1.540 real sec...
[M::mem_process_seqs] Processed 681568 reads in 155.892 CPU sec, 4.951 real sec
[M::mem_process_seqs] Processed 681542 reads in 100.738 CPU sec, 3.187 real sec
sorting block 26, #reads = 1071329 mem = 536881233 peak = 994086912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 277171, 6, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 216, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (259.68, 141.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 347, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (361.67, 170.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 317, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1069)
[M::mem_pestat] mean and std.dev: (313.71, 153.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1363)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681924 sequences (100000148 bp) in 2.805 CPU sec, 2.953 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 277853, 7, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 239, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (241.21, 112.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 338, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (354.14, 168.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 272, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (264.38, 117.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681992 sequences (100000034 bp) in 1.502 CPU sec, 1.506 real sec...
[M::mem_process_seqs] Processed 680626 reads in 157.446 CPU sec, 5.001 real sec
[M::mem_process_seqs] Processed 681756 reads in 102.782 CPU sec, 3.253 real sec
sorting block 27, #reads = 1069655 mem = 536879003 peak = 994086912
[M::process] read 681674 sequences (100000146 bp) in 2.379 CPU sec, 2.525 real sec...
[M::process] read 681196 sequences (100000278 bp) in 1.450 CPU sec, 1.443 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 277349, 2, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 240, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (272.59, 139.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1082)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 334, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (350.28, 166.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 257, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (263.40, 125.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 914)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681992 reads in 96.357 CPU sec, 3.063 real sec
sorting block 28, #reads = 1069317 mem = 536879411 peak = 994086912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 277949, 7, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 249, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501)
[M::mem_pestat] mean and std.dev: (225.00, 69.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 606)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 336, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (351.81, 166.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 235, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489)
[M::mem_pestat] mean and std.dev: (218.92, 57.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 594)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680970 sequences (100000106 bp) in 1.494 CPU sec, 1.498 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 277930, 5, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 233, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (273.26, 158.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 338, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (353.63, 167.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 325, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (358.73, 211.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681924 reads in 258.667 CPU sec, 8.250 real sec
[M::mem_process_seqs] Processed 681674 reads in 122.810 CPU sec, 3.940 real sec
sorting block 29, #reads = 1069574 mem = 536878907 peak = 994086912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 277799, 10, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 208, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (237.83, 132.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 342, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (358.12, 170.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (398, 2761, 2761)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7487)
[M::mem_pestat] mean and std.dev: (2483.90, 2412.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12134)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 348, 771)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1921)
[M::mem_pestat] mean and std.dev: (404.17, 296.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2496)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 688706 sequences (100000182 bp) in 2.471 CPU sec, 2.651 real sec...
[M::process] read 686338 sequences (100000054 bp) in 1.480 CPU sec, 1.484 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 277885, 8, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 252, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684)
[M::mem_pestat] mean and std.dev: (255.79, 105.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 849)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 346, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (363.04, 173.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 329, 559)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1139)
[M::mem_pestat] mean and std.dev: (380.86, 201.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1429)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681196 reads in 166.726 CPU sec, 5.312 real sec
sorting block 30, #reads = 1069186 mem = 536878910 peak = 994086912
[M::mem_process_seqs] Processed 680970 reads in 105.666 CPU sec, 3.365 real sec
sorting block 31, #reads = 1069509 mem = 536877714 peak = 994086912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 281230, 3, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 203, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (222.68, 105.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 305, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (328.39, 165.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 253, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (269.55, 114.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683906 sequences (100000252 bp) in 2.493 CPU sec, 2.658 real sec...
[M::process] read 683302 sequences (100000155 bp) in 1.480 CPU sec, 1.483 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 280069, 15, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 360, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1221)
[M::mem_pestat] mean and std.dev: (379.50, 262.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1569)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 319, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (338.98, 167.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (556, 694, 4405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12103)
[M::mem_pestat] mean and std.dev: (2068.53, 2371.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 15952)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 282, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (277.55, 91.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688706 reads in 177.252 CPU sec, 5.640 real sec
[M::process] read 683598 sequences (100000245 bp) in 2.166 CPU sec, 2.176 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 279269, 12, 50)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (251, 327, 624)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1370)
[M::mem_pestat] mean and std.dev: (403.95, 213.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1743)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 334, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (350.62, 170.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2385, 6315, 6808)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15654)
[M::mem_pestat] mean and std.dev: (4038.17, 2645.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 20077)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 342, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1180)
[M::mem_pestat] mean and std.dev: (366.85, 210.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1506)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683906 reads in 98.914 CPU sec, 3.234 real sec
sorting block 32, #reads = 1070469 mem = 536880136 peak = 994086912
[M::mem_process_seqs] Processed 686338 reads in 230.172 CPU sec, 7.429 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 278600, 6, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 221, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (218.71, 78.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 341, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (356.37, 172.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 451, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (333.03, 153.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683304 sequences (100000033 bp) in 2.302 CPU sec, 2.459 real sec...
[M::process] read 683150 sequences (100000071 bp) in 1.580 CPU sec, 1.577 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 279518, 5, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 363, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (316.90, 198.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 337, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (351.55, 168.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 230, 553)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1277)
[M::mem_pestat] mean and std.dev: (313.09, 233.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1639)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683302 reads in 190.641 CPU sec, 6.065 real sec
sorting block 33, #reads = 1069347 mem = 536883049 peak = 994086912
[M::process] read 682480 sequences (100000280 bp) in 1.633 CPU sec, 1.638 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 279258, 3, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 244, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (238.26, 115.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 338, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (352.83, 168.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 247, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (291.74, 139.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683304 reads in 100.796 CPU sec, 3.444 real sec
sorting block 34, #reads = 1066955 mem = 536880114 peak = 994086912
[M::mem_process_seqs] Processed 683598 reads in 235.537 CPU sec, 7.738 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 278287, 8, 51)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 268, 743)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1853)
[M::mem_pestat] mean and std.dev: (453.82, 392.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2408)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 336, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (351.32, 167.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 241, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (256.77, 130.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682438 sequences (100000282 bp) in 2.451 CPU sec, 2.640 real sec...
[M::process] read 681584 sequences (100000002 bp) in 1.490 CPU sec, 1.487 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 277833, 2, 56)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 281, 539)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1233)
[M::mem_pestat] mean and std.dev: (353.37, 173.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1580)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 342, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (356.41, 169.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 356, 546)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1188)
[M::mem_pestat] mean and std.dev: (340.32, 167.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1509)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683150 reads in 196.415 CPU sec, 6.255 real sec
sorting block 35, #reads = 1066434 mem = 536883491 peak = 994086912
[M::mem_process_seqs] Processed 682480 reads in 116.122 CPU sec, 3.696 real sec
sorting block 36, #reads = 1066657 mem = 536879255 peak = 1017262080
[M::process] read 681284 sequences (100000104 bp) in 2.225 CPU sec, 2.361 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 277765, 4, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 233, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (240.87, 110.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 901)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 340, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (355.32, 168.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 247, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (254.15, 109.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 777)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 688798 sequences (100000294 bp) in 1.510 CPU sec, 1.513 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 277941, 8, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (217, 333, 583)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1315)
[M::mem_pestat] mean and std.dev: (359.88, 246.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1681)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 346, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (360.12, 170.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 241, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (241.97, 114.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 747)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682438 reads in 180.829 CPU sec, 5.757 real sec
[M::mem_process_seqs] Processed 681584 reads in 113.725 CPU sec, 3.631 real sec
sorting block 37, #reads = 1064078 mem = 536880142 peak = 1017262080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 278465, 5, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (232, 332, 639)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1453)
[M::mem_pestat] mean and std.dev: (381.27, 250.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1860)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 349, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (364.29, 173.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (139, 214, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724)
[M::mem_pestat] mean and std.dev: (261.14, 136.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 919)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 688626 sequences (100000104 bp) in 2.375 CPU sec, 2.500 real sec...
[M::process] read 687716 sequences (100000003 bp) in 1.530 CPU sec, 1.530 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 280894, 0, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 306, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (262.00, 110.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 303, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (326.22, 163.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 248, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (264.23, 133.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681284 reads in 176.341 CPU sec, 5.615 real sec
[M::mem_process_seqs] Processed 688798 reads in 115.152 CPU sec, 3.669 real sec
sorting block 38, #reads = 1064872 mem = 536877148 peak = 1017262080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 280659, 9, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 200, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531)
[M::mem_pestat] mean and std.dev: (220.47, 86.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 655)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 304, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (322.98, 158.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 254, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (265.22, 120.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685998 sequences (100000206 bp) in 2.401 CPU sec, 2.558 real sec...
[M::process] read 685204 sequences (100000059 bp) in 1.480 CPU sec, 1.475 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 280895, 0, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (211, 329, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (337.96, 167.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 309, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (326.09, 157.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (252.57, 150.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688626 reads in 180.273 CPU sec, 5.738 real sec
sorting block 39, #reads = 1064436 mem = 536879643 peak = 1017262080
[M::mem_process_seqs] Processed 687716 reads in 116.723 CPU sec, 3.727 real sec
sorting block 40, #reads = 1063101 mem = 536878828 peak = 1017262080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 280615, 3, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 228, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1236)
[M::mem_pestat] mean and std.dev: (325.96, 259.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1603)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 320, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (335.86, 161.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 328, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (316.47, 127.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684854 sequences (100000198 bp) in 2.874 CPU sec, 3.002 real sec...
[M::process] read 684260 sequences (100000073 bp) in 1.990 CPU sec, 1.987 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 280231, 1, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 256, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (283.18, 143.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 324, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (339.61, 162.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 208, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573)
[M::mem_pestat] mean and std.dev: (206.72, 75.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 709)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685998 reads in 199.997 CPU sec, 6.350 real sec
[M::mem_process_seqs] Processed 685204 reads in 136.113 CPU sec, 4.321 real sec
sorting block 41, #reads = 1065464 mem = 536878295 peak = 1017262080
[M::process] read 682724 sequences (100000255 bp) in 2.750 CPU sec, 2.929 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 280130, 7, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 249, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (270.19, 104.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 325, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (341.24, 164.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 248, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (268.33, 118.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 981)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682480 sequences (100000221 bp) in 1.800 CPU sec, 1.793 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 279794, 4, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 268, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (253.49, 86.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 331, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (347.78, 168.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 298, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (297.39, 136.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1315)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 278528, 5, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 286, 803)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2059)
[M::mem_pestat] mean and std.dev: (485.74, 455.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2687)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 341, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (357.61, 173.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 281, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (297.68, 141.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684854 reads in 296.610 CPU sec, 9.468 real sec
[M::mem_process_seqs] Processed 684260 reads in 221.341 CPU sec, 7.096 real sec
sorting block 42, #reads = 1064761 mem = 536878231 peak = 1017262080
[M::mem_process_seqs] Processed 682724 reads in 243.930 CPU sec, 7.836 real sec
[M::process] read 681578 sequences (100000179 bp) in 2.851 CPU sec, 3.014 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 278803, 11, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 329, 573)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1367)
[M::mem_pestat] mean and std.dev: (406.02, 280.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1764)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 347, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (363.99, 177.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (551, 551, 1160)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2378)
[M::mem_pestat] mean and std.dev: (717.20, 375.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2987)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 299, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (302.64, 138.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 43, #reads = 1066457 mem = 536883800 peak = 1017262080
[M::process] read 683808 sequences (100000264 bp) in 2.324 CPU sec, 2.446 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 278596, 12, 56)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 229, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (232.40, 99.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 351, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (366.51, 177.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (456, 1494, 2151)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5541)
[M::mem_pestat] mean and std.dev: (1369.09, 1052.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7236)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 358, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (331.76, 151.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 686892 sequences (100000092 bp) in 1.830 CPU sec, 1.823 real sec...
[M::mem_process_seqs] Processed 682480 reads in 212.522 CPU sec, 6.767 real sec
[M::process] read 685028 sequences (100000283 bp) in 1.965 CPU sec, 1.971 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 279706, 6, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 225, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (227.78, 107.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 335, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (354.15, 174.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 281, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (246.75, 82.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 759)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683808 reads in 127.262 CPU sec, 4.158 real sec
sorting block 44, #reads = 1065389 mem = 536878732 peak = 1017262080
[M::mem_process_seqs] Processed 681578 reads in 295.081 CPU sec, 9.482 real sec
sorting block 45, #reads = 1062091 mem = 536878647 peak = 1031557120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 280617, 7, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 244, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (238.17, 89.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 720)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 315, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (335.48, 166.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 352, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (307.04, 127.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 880)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683840 sequences (100000038 bp) in 2.736 CPU sec, 2.902 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 279722, 13, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 246, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860)
[M::mem_pestat] mean and std.dev: (284.39, 157.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1085)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 324, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (342.23, 167.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (670, 889, 1474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3082)
[M::mem_pestat] mean and std.dev: (913.08, 424.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3886)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 307, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037)
[M::mem_pestat] mean and std.dev: (281.30, 137.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1326)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 683514 sequences (100000113 bp) in 1.912 CPU sec, 1.920 real sec...
[M::mem_process_seqs] Processed 686892 reads in 224.047 CPU sec, 7.119 real sec
[M::mem_process_seqs] Processed 685028 reads in 130.294 CPU sec, 4.345 real sec
sorting block 46, #reads = 1067916 mem = 536876407 peak = 1031557120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 279363, 10, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 309, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (356.87, 259.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1393)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 333, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (350.82, 171.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (116, 1160, 1217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3419)
[M::mem_pestat] mean and std.dev: (823.40, 503.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4520)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 250, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (276.12, 125.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 683494 sequences (100000111 bp) in 2.605 CPU sec, 2.755 real sec...
[M::process] read 682610 sequences (100000253 bp) in 1.830 CPU sec, 1.830 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 279465, 13, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 298, 796)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2032)
[M::mem_pestat] mean and std.dev: (399.17, 297.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2650)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 335, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (352.44, 171.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (882, 1338, 6041)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16359)
[M::mem_pestat] mean and std.dev: (2330.85, 2235.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 21518)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 270, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759)
[M::mem_pestat] mean and std.dev: (282.20, 103.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683840 reads in 205.771 CPU sec, 6.773 real sec
[M::mem_process_seqs] Processed 683514 reads in 129.844 CPU sec, 4.152 real sec
sorting block 47, #reads = 1069641 mem = 536879860 peak = 1031557120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 279511, 5, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (250, 350, 883)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2149)
[M::mem_pestat] mean and std.dev: (348.59, 199.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2782)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 335, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (351.99, 170.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 251, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (252.54, 107.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682384 sequences (100000072 bp) in 2.489 CPU sec, 2.623 real sec...
[M::process] read 682344 sequences (100000008 bp) in 1.770 CPU sec, 1.766 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 279174, 5, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (216, 300, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (299.61, 134.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 341, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (356.12, 171.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 347, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (295.33, 103.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683494 reads in 199.230 CPU sec, 6.354 real sec
sorting block 48, #reads = 1068849 mem = 536889133 peak = 1031557120
[M::mem_process_seqs] Processed 682610 reads in 130.321 CPU sec, 4.407 real sec
sorting block 49, #reads = 1067958 mem = 536879026 peak = 1031557120
[M::process] read 682226 sequences (100000065 bp) in 2.107 CPU sec, 2.252 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 278889, 3, 52)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 138, 229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423)
[M::mem_pestat] mean and std.dev: (167.28, 69.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 520)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 344, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (360.23, 174.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 314, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (294.92, 115.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 784)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682264 sequences (100000236 bp) in 1.480 CPU sec, 1.482 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 278499, 6, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 287, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1107)
[M::mem_pestat] mean and std.dev: (283.75, 148.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1428)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 343, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (360.04, 174.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 340, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (312.02, 125.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 278258, 4, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 292, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (308.35, 186.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 343, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (359.42, 173.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 368, 553)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1211)
[M::mem_pestat] mean and std.dev: (366.55, 193.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1540)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682384 reads in 286.421 CPU sec, 9.414 real sec
[M::mem_process_seqs] Processed 682344 reads in 214.571 CPU sec, 6.891 real sec
[M::mem_process_seqs] Processed 682226 reads in 238.018 CPU sec, 7.661 real sec
sorting block 50, #reads = 1066681 mem = 536878461 peak = 1031557120
[M::process] read 682098 sequences (100000095 bp) in 2.441 CPU sec, 2.596 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278679, 2, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 191, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1091)
[M::mem_pestat] mean and std.dev: (262.63, 176.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1406)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 342, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (360.45, 175.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 344, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (334.22, 155.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 51, #reads = 1064954 mem = 536882177 peak = 1031557120
[M::process] read 688672 sequences (100000292 bp) in 2.454 CPU sec, 2.560 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278950, 6, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (224, 359, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (317.42, 113.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 344, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (362.31, 176.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 347, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (330.12, 167.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682264 reads in 206.653 CPU sec, 6.571 real sec
[M::process] read 688514 sequences (100000226 bp) in 2.005 CPU sec, 2.011 real sec...
[M::mem_process_seqs] Processed 682098 reads in 138.592 CPU sec, 4.607 real sec
sorting block 52, #reads = 1064066 mem = 536878025 peak = 1031557120
[M::process] read 686994 sequences (100000075 bp) in 2.674 CPU sec, 2.846 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 281039, 6, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 198, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (260.36, 167.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1332)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 304, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (328.17, 166.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 232, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577)
[M::mem_pestat] mean and std.dev: (222.97, 108.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 718)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685068 sequences (100000170 bp) in 1.888 CPU sec, 1.897 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 281077, 4, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 256, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (267.39, 132.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 303, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (321.95, 157.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 313, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1210)
[M::mem_pestat] mean and std.dev: (325.66, 239.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1557)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 280761, 8, 58)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (196, 218, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (239.28, 114.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 772)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 314, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (331.42, 161.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 261, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (304.39, 154.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688672 reads in 326.354 CPU sec, 10.662 real sec
[M::mem_process_seqs] Processed 688514 reads in 244.605 CPU sec, 7.856 real sec
sorting block 53, #reads = 1059259 mem = 536880623 peak = 1031557120
[M::mem_process_seqs] Processed 686994 reads in 270.945 CPU sec, 8.725 real sec
[M::process] read 684932 sequences (100000101 bp) in 3.020 CPU sec, 3.223 real sec...
sorting block 54, #reads = 1062441 mem = 536881033 peak = 1031557120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 279930, 9, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 308, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1049)
[M::mem_pestat] mean and std.dev: (320.40, 173.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1332)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 324, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (341.88, 165.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 273, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075)
[M::mem_pestat] mean and std.dev: (314.50, 167.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1363)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683100 sequences (100000209 bp) in 2.335 CPU sec, 2.451 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 279389, 5, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 243, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (252.90, 142.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1321)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 322, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (339.46, 163.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 241, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (285.31, 127.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683794 sequences (100000167 bp) in 1.900 CPU sec, 1.895 real sec...
[M::mem_process_seqs] Processed 685068 reads in 228.312 CPU sec, 7.270 real sec
[M::mem_process_seqs] Processed 684932 reads in 148.009 CPU sec, 4.693 real sec
sorting block 55, #reads = 1062511 mem = 536879500 peak = 1031557120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 279050, 7, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 262, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (301.65, 178.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 335, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (349.41, 165.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 252, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1310)
[M::mem_pestat] mean and std.dev: (351.61, 284.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1699)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683094 sequences (100000005 bp) in 2.755 CPU sec, 2.916 real sec...
[M::process] read 682532 sequences (100000148 bp) in 1.860 CPU sec, 1.861 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 279054, 4, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 193, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (275.83, 192.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 330, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (346.41, 165.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 308, 643)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1507)
[M::mem_pestat] mean and std.dev: (342.32, 191.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1939)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683100 reads in 219.533 CPU sec, 7.078 real sec
sorting block 56, #reads = 1063247 mem = 536879063 peak = 1031557120
[M::mem_process_seqs] Processed 683794 reads in 144.478 CPU sec, 4.614 real sec
[M::process] read 682108 sequences (100000094 bp) in 2.442 CPU sec, 2.583 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278243, 5, 60)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 305, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (269.76, 101.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 335, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (350.98, 167.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 276, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (331.07, 175.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1296)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683094 reads in 121.588 CPU sec, 3.939 real sec
sorting block 57, #reads = 1063954 mem = 536879021 peak = 1031557120
[M::process] read 681302 sequences (100000001 bp) in 2.409 CPU sec, 2.543 real sec...
[M::process] read 52534 sequences (7689828 bp) in 0.490 CPU sec, 0.493 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 278524, 4, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 303, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (300.83, 148.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 341, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (359.38, 174.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 299, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (275.67, 79.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 278494, 11, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 257, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1196)
[M::mem_pestat] mean and std.dev: (318.40, 203.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1535)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 337, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (354.69, 170.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 594, 1203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3309)
[M::mem_pestat] mean and std.dev: (627.80, 605.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4362)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 270, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (287.32, 142.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1096)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682108 reads in 233.873 CPU sec, 7.542 real sec
[M::mem_process_seqs] Processed 682532 reads in 256.671 CPU sec, 8.388 real sec
sorting block 58, #reads = 1064793 mem = 536877544 peak = 1031557120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 21418, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 336, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (351.82, 169.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 278670, 1, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 237, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (233.62, 100.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 348, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (365.04, 175.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 303, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (297.81, 91.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 52534 reads in 10.370 CPU sec, 0.337 real sec
[M::mem_process_seqs] Processed 681302 reads in 122.509 CPU sec, 4.094 real sec
sorting block 59, #reads = 1066805 mem = 536878831 peak = 1034944512
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 64257874
overall: 70177439744 mem 10558.005 user 292.294 sys 1077.734 real
sorting block 60, #reads = 565998 mem = 285500542 peak = 1034944512
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1029_N5D-T1-TDNA-1_A23MHWWLT4_1.L007\tPU:L007\tSM:1029_N5D-T1-TDNA-1_A23MHWWLT4_1\tLB:1029_N5D-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0003.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0003.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 1077.794 sec; CPU: 10850.328 sec
uniq header count 1
merging 61 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 4001913076
output reads: 64636293
bam_mem_sort: 61 calls 30.824 user 0.359 sys 31.303 real
bam_write: 61 calls 24.148 user 0.938 sys 25.304 real
execute: 1 calls 19.805 user 1.214 sys 1104.471 real
merge_files: 1 calls 19.794 user 1.186 sys 26.287 real
parse_chunk: 969 calls 37.480 user 9.477 sys 47.603 real
read_chunk: 2617 calls 26.473 user 22.630 sys 307.615 real
sort_block: 1 calls 17.998 user 21.881 sys 295.367 real
write_chunk: 1646 calls 19.387 user 1.041 sys 25.502 real
overall: 2926514176 mem 985.874 user 44.921 sys 1104.492 real
ls: cannot access '*.cram': No such file or directory