cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1047_JG-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1679.144 CPU sec, 716.361 real sec
[M::process] read 700444 sequences (100000137 bp) in 0.997 CPU sec, 1.007 real sec...
[M::process] read 699380 sequences (100000035 bp) in 1.210 CPU sec, 1.202 real sec...
[M::process] read 698600 sequences (100000287 bp) in 1.182 CPU sec, 1.201 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291538, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (246.93, 118.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698190 sequences (100000167 bp) in 1.143 CPU sec, 1.160 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290995, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (253.32, 122.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290960, 8, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.62, 120.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700444 reads in 219.597 CPU sec, 7.049 real sec
[M::mem_process_seqs] Processed 698600 reads in 142.092 CPU sec, 4.608 real sec
[M::mem_process_seqs] Processed 699380 reads in 154.036 CPU sec, 5.011 real sec
sorting block 0, #reads = 1093963 mem = 536881579 peak = 584953856
[M::process] read 697382 sequences (100000221 bp) in 1.790 CPU sec, 1.958 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290481, 2, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1219)
[M::mem_pestat] mean and std.dev: (225.62, 112.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1569)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (254.66, 123.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 640, 972)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2560)
[M::mem_pestat] mean and std.dev: (690.50, 588.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3354)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 697420 sequences (100000236 bp) in 1.312 CPU sec, 1.352 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290848, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 233, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (257.28, 124.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 868)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698190 reads in 131.626 CPU sec, 4.208 real sec
sorting block 1, #reads = 1093801 mem = 536879289 peak = 865353728
[M::process] read 698312 sequences (100000085 bp) in 1.187 CPU sec, 1.194 real sec...
[M::mem_process_seqs] Processed 697382 reads in 83.013 CPU sec, 2.632 real sec
sorting block 2, #reads = 1093523 mem = 536880749 peak = 903081984
[M::process] read 698224 sequences (100000024 bp) in 1.644 CPU sec, 1.737 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290663, 7, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (271, 344, 700)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1558)
[M::mem_pestat] mean and std.dev: (305.89, 159.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1987)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 233, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (258.64, 125.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 873)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 615, 1089)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2793)
[M::mem_pestat] mean and std.dev: (598.79, 367.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3645)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 697580 sequences (100000298 bp) in 1.188 CPU sec, 1.202 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290929, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (254.74, 123.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697420 reads in 130.940 CPU sec, 4.184 real sec
[M::mem_process_seqs] Processed 698312 reads in 81.395 CPU sec, 2.607 real sec
sorting block 3, #reads = 1094141 mem = 536880332 peak = 903081984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291088, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (254.40, 123.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697490 sequences (100000083 bp) in 1.765 CPU sec, 1.849 real sec...
[M::process] read 696504 sequences (100000056 bp) in 1.190 CPU sec, 1.195 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 290744, 9, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (231, 421, 584)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1290)
[M::mem_pestat] mean and std.dev: (413.13, 200.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1643)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (254.89, 122.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698224 reads in 131.118 CPU sec, 4.188 real sec
[M::mem_process_seqs] Processed 697580 reads in 81.129 CPU sec, 2.590 real sec
sorting block 4, #reads = 1094578 mem = 536877012 peak = 903081984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290558, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (255.00, 122.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 852)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696998 sequences (100000259 bp) in 1.773 CPU sec, 1.915 real sec...
[M::process] read 697504 sequences (100000221 bp) in 1.260 CPU sec, 1.256 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290324, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (255.08, 121.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697490 reads in 132.801 CPU sec, 4.244 real sec
sorting block 5, #reads = 1094638 mem = 536877546 peak = 903081984
[M::mem_process_seqs] Processed 696504 reads in 82.682 CPU sec, 2.634 real sec
sorting block 6, #reads = 1092694 mem = 536881891 peak = 903081984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290886, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.19, 118.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696388 sequences (100000246 bp) in 1.963 CPU sec, 2.099 real sec...
[M::process] read 696176 sequences (100000211 bp) in 1.260 CPU sec, 1.263 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290000, 10, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (282, 555, 642)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1362)
[M::mem_pestat] mean and std.dev: (386.20, 194.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1722)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (250.94, 117.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (418, 418, 1284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3016)
[M::mem_pestat] mean and std.dev: (602.88, 401.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3882)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 696998 reads in 138.082 CPU sec, 4.405 real sec
[M::mem_process_seqs] Processed 697504 reads in 86.855 CPU sec, 2.769 real sec
sorting block 7, #reads = 1089680 mem = 536877792 peak = 903081984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290324, 2, 17)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (252.84, 119.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 322, 5306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15532)
[M::mem_pestat] mean and std.dev: (1572.47, 2102.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 20645)
[M::mem_pestat] skip orientation RR
[M::process] read 696012 sequences (100000051 bp) in 1.751 CPU sec, 1.886 real sec...
[M::process] read 695692 sequences (100000241 bp) in 1.290 CPU sec, 1.287 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 289925, 10, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (252.99, 118.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 458, 1042)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2854)
[M::mem_pestat] mean and std.dev: (544.90, 458.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3760)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 696388 reads in 133.546 CPU sec, 4.274 real sec
[M::mem_process_seqs] Processed 696176 reads in 83.289 CPU sec, 2.651 real sec
sorting block 8, #reads = 1092026 mem = 536880112 peak = 903081984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 290352, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.48, 120.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 694862 sequences (100000115 bp) in 2.073 CPU sec, 2.212 real sec...
[M::process] read 698776 sequences (100000238 bp) in 1.330 CPU sec, 1.323 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 289855, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (254.20, 119.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696012 reads in 135.207 CPU sec, 4.315 real sec
[M::mem_process_seqs] Processed 695692 reads in 84.475 CPU sec, 2.688 real sec
sorting block 9, #reads = 1091813 mem = 536878817 peak = 903081984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 289755, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 234, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (255.41, 119.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699048 sequences (100000234 bp) in 1.860 CPU sec, 2.017 real sec...
[M::process] read 698542 sequences (100000080 bp) in 1.320 CPU sec, 1.313 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290643, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (249.83, 118.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 694862 reads in 134.864 CPU sec, 4.313 real sec
sorting block 10, #reads = 1092048 mem = 536879839 peak = 903081984
[M::mem_process_seqs] Processed 698776 reads in 84.215 CPU sec, 2.682 real sec
sorting block 11, #reads = 1092239 mem = 536878070 peak = 903081984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290811, 2, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 351, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (252.00, 112.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.11, 120.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698388 sequences (100000220 bp) in 2.181 CPU sec, 2.344 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291074, 6, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (253.94, 123.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 234, 738)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1898)
[M::mem_pestat] mean and std.dev: (428.92, 340.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2478)
[M::mem_pestat] skip orientation RR
[M::process] read 697536 sequences (100000039 bp) in 1.340 CPU sec, 1.338 real sec...
[M::mem_process_seqs] Processed 699048 reads in 133.357 CPU sec, 4.254 real sec
[M::mem_process_seqs] Processed 698542 reads in 83.349 CPU sec, 2.650 real sec
sorting block 12, #reads = 1093290 mem = 536887909 peak = 903081984
[M::process] read 697248 sequences (100000144 bp) in 1.995 CPU sec, 2.167 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290977, 6, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (229, 230, 230)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (227, 232)
[M::mem_pestat] mean and std.dev: (229.67, 0.47)
[M::mem_pestat] low and high boundaries for proper pairs: (226, 233)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (257.02, 125.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 868)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 204, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (299.50, 190.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 697396 sequences (100000041 bp) in 1.340 CPU sec, 1.338 real sec...
[M::mem_process_seqs] Processed 697536 reads in 77.707 CPU sec, 2.469 real sec
sorting block 13, #reads = 1094208 mem = 536885143 peak = 903081984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290609, 6, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 224, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (234.55, 91.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684)
[M::mem_pestat] mean and std.dev: (255.01, 124.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290848, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 233, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (259.37, 126.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 880)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698388 reads in 206.323 CPU sec, 6.608 real sec
[M::process] read 697986 sequences (100000133 bp) in 1.427 CPU sec, 1.436 real sec...
[M::mem_process_seqs] Processed 697248 reads in 96.068 CPU sec, 3.080 real sec
sorting block 14, #reads = 1094292 mem = 536877397 peak = 903081984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290936, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 233, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (258.02, 125.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 872)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697466 sequences (100000132 bp) in 2.012 CPU sec, 2.172 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290864, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (254.79, 123.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697118 sequences (100000077 bp) in 1.310 CPU sec, 1.306 real sec...
[M::mem_process_seqs] Processed 697396 reads in 134.780 CPU sec, 4.313 real sec
[M::mem_process_seqs] Processed 697986 reads in 84.948 CPU sec, 2.701 real sec
sorting block 15, #reads = 1094274 mem = 536880010 peak = 903081984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290885, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (254.84, 122.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696988 sequences (100000215 bp) in 1.864 CPU sec, 2.004 real sec...
[M::process] read 697312 sequences (100000221 bp) in 1.330 CPU sec, 1.329 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290576, 4, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (112, 208, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1201)
[M::mem_pestat] mean and std.dev: (289.91, 255.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1564)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (253.95, 121.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 408, 639)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1449)
[M::mem_pestat] mean and std.dev: (418.00, 247.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1854)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697466 reads in 133.984 CPU sec, 4.279 real sec
[M::mem_process_seqs] Processed 697118 reads in 83.090 CPU sec, 2.642 real sec
sorting block 16, #reads = 1094342 mem = 536875892 peak = 903081984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290278, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.10, 120.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 301, 687)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1765)
[M::mem_pestat] mean and std.dev: (370.50, 261.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2304)
[M::mem_pestat] skip orientation RR
[M::process] read 698484 sequences (100000151 bp) in 2.236 CPU sec, 2.376 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290541, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.73, 119.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697250 sequences (100000221 bp) in 1.300 CPU sec, 1.300 real sec...
[M::mem_process_seqs] Processed 696988 reads in 132.435 CPU sec, 4.215 real sec
sorting block 17, #reads = 1093979 mem = 536881633 peak = 903081984
[M::mem_process_seqs] Processed 697312 reads in 81.875 CPU sec, 2.598 real sec
sorting block 18, #reads = 1093251 mem = 536881437 peak = 961282048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290712, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.03, 118.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696496 sequences (100000165 bp) in 2.358 CPU sec, 2.539 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290452, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.25, 119.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698484 reads in 133.942 CPU sec, 4.258 real sec
[M::process] read 696184 sequences (100000244 bp) in 1.350 CPU sec, 1.348 real sec...
[M::mem_process_seqs] Processed 697250 reads in 83.802 CPU sec, 2.650 real sec
sorting block 19, #reads = 1090794 mem = 536878139 peak = 961282048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290654, 9, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (253.03, 119.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 222, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485)
[M::mem_pestat] mean and std.dev: (238.56, 66.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 582)
[M::mem_pestat] skip orientation RR
[M::process] read 695832 sequences (100000160 bp) in 2.203 CPU sec, 2.317 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290048, 8, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.20, 119.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 1049, 3519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10153)
[M::mem_pestat] mean and std.dev: (1434.75, 1506.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 13470)
[M::mem_pestat] skip orientation RR
[M::process] read 695546 sequences (100000067 bp) in 1.320 CPU sec, 1.318 real sec...
[M::mem_process_seqs] Processed 696496 reads in 129.134 CPU sec, 4.100 real sec
[M::mem_process_seqs] Processed 696184 reads in 81.782 CPU sec, 2.599 real sec
sorting block 20, #reads = 1091937 mem = 536878920 peak = 961282048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 289889, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (254.56, 119.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698772 sequences (100000058 bp) in 2.208 CPU sec, 2.316 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 289597, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 234, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (256.30, 121.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699248 sequences (100000075 bp) in 1.330 CPU sec, 1.327 real sec...
[M::mem_process_seqs] Processed 695832 reads in 129.374 CPU sec, 4.106 real sec
sorting block 21, #reads = 1093499 mem = 536879405 peak = 961282048
[M::mem_process_seqs] Processed 695546 reads in 81.830 CPU sec, 2.591 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290806, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (250.73, 120.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 477, 1427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3941)
[M::mem_pestat] mean and std.dev: (706.40, 688.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5198)
[M::mem_pestat] skip orientation RR
sorting block 22, #reads = 1091310 mem = 536879143 peak = 961282048
[M::process] read 698136 sequences (100000028 bp) in 2.141 CPU sec, 2.280 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291029, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (251.16, 121.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (357, 357, 1020)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2346)
[M::mem_pestat] mean and std.dev: (448.80, 297.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3009)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698772 reads in 131.319 CPU sec, 4.182 real sec
[M::process] read 697424 sequences (100000228 bp) in 1.375 CPU sec, 1.382 real sec...
[M::mem_process_seqs] Processed 699248 reads in 82.921 CPU sec, 2.621 real sec
sorting block 23, #reads = 1092519 mem = 536881685 peak = 961282048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290898, 9, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 180, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690)
[M::mem_pestat] mean and std.dev: (277.36, 161.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (253.88, 122.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 696958 sequences (100000102 bp) in 2.246 CPU sec, 2.357 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291024, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (256.16, 123.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 860)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696432 sequences (100000126 bp) in 1.340 CPU sec, 1.337 real sec...
[M::mem_process_seqs] Processed 698136 reads in 130.332 CPU sec, 4.143 real sec
[M::mem_process_seqs] Processed 697424 reads in 82.362 CPU sec, 2.602 real sec
sorting block 24, #reads = 1094127 mem = 536879510 peak = 961282048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290351, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 233, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (258.55, 125.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 873)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697140 sequences (100000035 bp) in 2.038 CPU sec, 2.145 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290577, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 235, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (260.54, 126.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 885)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698046 sequences (100000078 bp) in 1.320 CPU sec, 1.317 real sec...
[M::mem_process_seqs] Processed 696958 reads in 129.352 CPU sec, 4.117 real sec
[M::mem_process_seqs] Processed 696432 reads in 81.418 CPU sec, 2.578 real sec
sorting block 25, #reads = 1093582 mem = 536877270 peak = 961282048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290781, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 233, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (258.83, 126.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 873)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697120 sequences (100000291 bp) in 1.998 CPU sec, 2.123 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291243, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (253.60, 122.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697168 sequences (100000162 bp) in 1.310 CPU sec, 1.309 real sec...
[M::mem_process_seqs] Processed 697140 reads in 129.817 CPU sec, 4.132 real sec
sorting block 26, #reads = 1093969 mem = 536880399 peak = 961282048
[M::mem_process_seqs] Processed 698046 reads in 82.367 CPU sec, 2.607 real sec
sorting block 27, #reads = 1093451 mem = 536879423 peak = 1008046080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290700, 4, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (255.05, 122.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 852)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 334, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (87, 552)
[M::mem_pestat] mean and std.dev: (295.64, 66.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 645)
[M::mem_pestat] skip orientation RR
[M::process] read 696964 sequences (100000099 bp) in 2.287 CPU sec, 2.409 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290459, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.32, 122.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696970 sequences (100000237 bp) in 1.320 CPU sec, 1.326 real sec...
[M::mem_process_seqs] Processed 697120 reads in 129.763 CPU sec, 4.119 real sec
[M::mem_process_seqs] Processed 697168 reads in 81.993 CPU sec, 2.591 real sec
sorting block 28, #reads = 1094009 mem = 536880855 peak = 1008046080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290571, 9, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (252.98, 120.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 854, 1127)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3101)
[M::mem_pestat] mean and std.dev: (498.75, 429.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4088)
[M::mem_pestat] skip orientation RR
[M::process] read 697640 sequences (100000139 bp) in 2.407 CPU sec, 2.538 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290628, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (252.91, 120.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696964 reads in 130.266 CPU sec, 4.147 real sec
[M::process] read 696930 sequences (100000217 bp) in 1.403 CPU sec, 1.422 real sec...
[M::mem_process_seqs] Processed 696970 reads in 82.743 CPU sec, 2.611 real sec
sorting block 29, #reads = 1093228 mem = 536880492 peak = 1008046080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290393, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.59, 119.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696608 sequences (100000220 bp) in 2.055 CPU sec, 2.169 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290927, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.25, 120.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 238, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (242.60, 73.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] skip orientation RR
[M::process] read 696418 sequences (100000130 bp) in 1.290 CPU sec, 1.288 real sec...
[M::mem_process_seqs] Processed 697640 reads in 131.945 CPU sec, 4.205 real sec
sorting block 30, #reads = 1092973 mem = 536879663 peak = 1008046080
[M::mem_process_seqs] Processed 696930 reads in 82.303 CPU sec, 2.605 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290393, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (253.10, 120.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 31, #reads = 1093569 mem = 536879685 peak = 1008046080
[M::process] read 696048 sequences (100000172 bp) in 2.013 CPU sec, 2.129 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290588, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (254.01, 120.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 695996 sequences (100000235 bp) in 1.330 CPU sec, 1.327 real sec...
[M::mem_process_seqs] Processed 696608 reads in 129.959 CPU sec, 4.138 real sec
[M::mem_process_seqs] Processed 696418 reads in 82.043 CPU sec, 2.593 real sec
sorting block 32, #reads = 1093398 mem = 536879221 peak = 1008046080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290403, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (255.04, 121.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697618 sequences (100000063 bp) in 2.194 CPU sec, 2.303 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 289899, 6, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 310, 586)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1388)
[M::mem_pestat] mean and std.dev: (391.55, 265.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1789)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (255.51, 121.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698844 sequences (100000061 bp) in 1.310 CPU sec, 1.312 real sec...
[M::mem_process_seqs] Processed 696048 reads in 130.290 CPU sec, 4.139 real sec
[M::mem_process_seqs] Processed 695996 reads in 82.803 CPU sec, 2.620 real sec
sorting block 33, #reads = 1093043 mem = 536881620 peak = 1008046080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290838, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (252.59, 120.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698156 sequences (100000078 bp) in 2.129 CPU sec, 2.256 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290876, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.51, 121.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697600 sequences (100000259 bp) in 1.280 CPU sec, 1.289 real sec...
[M::mem_process_seqs] Processed 697618 reads in 131.021 CPU sec, 4.167 real sec
[M::mem_process_seqs] Processed 698844 reads in 81.915 CPU sec, 2.593 real sec
sorting block 34, #reads = 1090984 mem = 536876218 peak = 1008046080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 290489, 9, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (254.15, 123.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697054 sequences (100000173 bp) in 2.252 CPU sec, 2.362 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290501, 3, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (120, 308, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (249.11, 142.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (256.77, 125.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 864)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 695884 sequences (100000215 bp) in 1.290 CPU sec, 1.297 real sec...
[M::mem_process_seqs] Processed 698156 reads in 128.962 CPU sec, 4.097 real sec
sorting block 35, #reads = 1094619 mem = 536877111 peak = 1008046080
[M::mem_process_seqs] Processed 697600 reads in 81.526 CPU sec, 2.578 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290889, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (257.89, 125.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 869)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 36, #reads = 1093633 mem = 536879672 peak = 1012334592
[M::process] read 695798 sequences (100000037 bp) in 2.018 CPU sec, 2.147 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290559, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 236, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702)
[M::mem_pestat] mean and std.dev: (261.31, 126.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697054 reads in 127.708 CPU sec, 4.066 real sec
[M::process] read 698016 sequences (100000146 bp) in 1.350 CPU sec, 1.344 real sec...
[M::mem_process_seqs] Processed 695884 reads in 81.158 CPU sec, 2.563 real sec
sorting block 37, #reads = 1093859 mem = 536880267 peak = 1012334592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290240, 2, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 562, 962)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2498)
[M::mem_pestat] mean and std.dev: (535.64, 483.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3266)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 236, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705)
[M::mem_pestat] mean and std.dev: (261.71, 127.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697026 sequences (100000301 bp) in 2.245 CPU sec, 2.358 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 291162, 2, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 287, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (248.81, 90.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 743)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (253.52, 122.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697104 sequences (100000013 bp) in 1.310 CPU sec, 1.309 real sec...
[M::mem_process_seqs] Processed 695798 reads in 128.138 CPU sec, 4.071 real sec
[M::mem_process_seqs] Processed 698016 reads in 81.426 CPU sec, 2.575 real sec
sorting block 38, #reads = 1094168 mem = 536878963 peak = 1012334592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290768, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (256.03, 123.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 856)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697196 sequences (100000080 bp) in 2.302 CPU sec, 2.428 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290242, 3, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 154, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485)
[M::mem_pestat] mean and std.dev: (172.44, 69.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 604)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.43, 122.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 337, 3756)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10896)
[M::mem_pestat] mean and std.dev: (1603.20, 2204.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14466)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 696926 sequences (100000222 bp) in 1.310 CPU sec, 1.311 real sec...
[M::mem_process_seqs] Processed 697026 reads in 129.238 CPU sec, 4.104 real sec
[M::mem_process_seqs] Processed 697104 reads in 81.146 CPU sec, 2.565 real sec
sorting block 39, #reads = 1094222 mem = 536883858 peak = 1012334592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290942, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (252.96, 121.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 40, #reads = 1093593 mem = 536878265 peak = 1023299584
[M::process] read 696712 sequences (100000257 bp) in 2.221 CPU sec, 2.363 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 290232, 2, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (225, 233, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (230.67, 67.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.82, 120.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697196 reads in 130.173 CPU sec, 4.150 real sec
[M::process] read 696858 sequences (100000283 bp) in 1.433 CPU sec, 1.448 real sec...
[M::mem_process_seqs] Processed 696926 reads in 82.496 CPU sec, 2.610 real sec
sorting block 41, #reads = 1093686 mem = 536879698 peak = 1023299584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290176, 3, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.50, 120.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 257, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1126)
[M::mem_pestat] mean and std.dev: (257.00, 129.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1454)
[M::mem_pestat] skip orientation RR
[M::process] read 697218 sequences (100000207 bp) in 2.103 CPU sec, 2.248 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290455, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.14, 121.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696712 reads in 130.141 CPU sec, 4.142 real sec
[M::process] read 696454 sequences (100000267 bp) in 1.310 CPU sec, 1.307 real sec...
[M::mem_process_seqs] Processed 696858 reads in 83.142 CPU sec, 2.631 real sec
sorting block 42, #reads = 1092669 mem = 536880661 peak = 1023299584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290652, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.49, 120.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 232, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521)
[M::mem_pestat] mean and std.dev: (228.67, 107.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 660)
[M::mem_pestat] skip orientation RR
[M::process] read 695538 sequences (100000279 bp) in 2.333 CPU sec, 2.456 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 290272, 4, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 222, 1168)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3230)
[M::mem_pestat] mean and std.dev: (651.87, 696.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4261)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (253.82, 120.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697218 reads in 130.855 CPU sec, 4.158 real sec
[M::process] read 695572 sequences (100000220 bp) in 1.350 CPU sec, 1.359 real sec...
[M::mem_process_seqs] Processed 696454 reads in 82.964 CPU sec, 2.624 real sec
sorting block 43, #reads = 1093213 mem = 536879541 peak = 1029115904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 289804, 3, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 337, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (346.08, 272.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1436)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (256.27, 121.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697246 sequences (100000155 bp) in 2.043 CPU sec, 2.162 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290119, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 234, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (257.50, 122.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 705430 sequences (100000107 bp) in 1.360 CPU sec, 1.358 real sec...
[M::mem_process_seqs] Processed 695538 reads in 130.068 CPU sec, 4.135 real sec
sorting block 44, #reads = 1092302 mem = 536880456 peak = 1029115904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290270, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (253.01, 121.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703854 sequences (100000123 bp) in 1.684 CPU sec, 1.693 real sec...
[M::mem_process_seqs] Processed 697246 reads in 77.679 CPU sec, 2.468 real sec
sorting block 45, #reads = 1091435 mem = 536876407 peak = 1032708096
[M::mem_process_seqs] Processed 695572 reads in 182.936 CPU sec, 5.814 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 293121, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 209, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (234.89, 114.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 772)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703436 sequences (100000127 bp) in 2.012 CPU sec, 2.131 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292648, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (237.90, 116.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 782)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (370, 387, 836)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1768)
[M::mem_pestat] mean and std.dev: (511.11, 326.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2234)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 705430 reads in 150.010 CPU sec, 4.757 real sec
[M::process] read 702072 sequences (100000202 bp) in 1.340 CPU sec, 1.340 real sec...
sorting block 46, #reads = 1092839 mem = 536878165 peak = 1032708096
[M::mem_process_seqs] Processed 703854 reads in 87.063 CPU sec, 2.753 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292583, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (240.24, 117.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 47, #reads = 1095323 mem = 536879502 peak = 1042386944
[M::process] read 701162 sequences (100000151 bp) in 2.626 CPU sec, 2.829 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292419, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (245.30, 120.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703436 reads in 137.868 CPU sec, 4.377 real sec
[M::process] read 702172 sequences (100000141 bp) in 1.602 CPU sec, 1.632 real sec...
[M::mem_process_seqs] Processed 702072 reads in 86.843 CPU sec, 2.740 real sec
sorting block 48, #reads = 1093543 mem = 536878579 peak = 1042386944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 292159, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (242, 337, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (339.79, 130.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (247.41, 121.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 703456 sequences (100000140 bp) in 1.989 CPU sec, 2.087 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292337, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (244.08, 119.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702658 sequences (100000031 bp) in 1.390 CPU sec, 1.388 real sec...
[M::mem_process_seqs] Processed 701162 reads in 134.745 CPU sec, 4.290 real sec
[M::mem_process_seqs] Processed 702172 reads in 85.346 CPU sec, 2.699 real sec
sorting block 49, #reads = 1094687 mem = 536880470 peak = 1042386944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292484, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (239.19, 116.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702054 sequences (100000088 bp) in 2.112 CPU sec, 2.215 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292853, 4, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 672, 1878)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5382)
[M::mem_pestat] mean and std.dev: (763.89, 774.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7134)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638)
[M::mem_pestat] mean and std.dev: (241.52, 117.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 800)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (911, 1962, 2468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5582)
[M::mem_pestat] mean and std.dev: (1816.00, 1391.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7381)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 700446 sequences (100000204 bp) in 1.350 CPU sec, 1.351 real sec...
[M::mem_process_seqs] Processed 703456 reads in 140.595 CPU sec, 4.479 real sec
[M::mem_process_seqs] Processed 702658 reads in 89.232 CPU sec, 2.824 real sec
sorting block 50, #reads = 1093987 mem = 536878715 peak = 1042386944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 292363, 4, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (214, 361, 599)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1369)
[M::mem_pestat] mean and std.dev: (398.42, 213.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1754)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (243.26, 118.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 702504 sequences (100000171 bp) in 2.087 CPU sec, 2.185 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292001, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 319, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (208.33, 100.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 919)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (246.87, 119.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 700182 sequences (100000008 bp) in 1.390 CPU sec, 1.395 real sec...
[M::mem_process_seqs] Processed 702054 reads in 139.632 CPU sec, 4.444 real sec
sorting block 51, #reads = 1092562 mem = 536884309 peak = 1070399488
[M::mem_process_seqs] Processed 700446 reads in 86.269 CPU sec, 2.730 real sec
sorting block 52, #reads = 1092884 mem = 536880174 peak = 1103368192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292384, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.95, 116.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699070 sequences (100000129 bp) in 2.415 CPU sec, 2.542 real sec...
[M::process] read 699064 sequences (100000226 bp) in 1.380 CPU sec, 1.379 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291543, 9, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (245.35, 117.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 238, 259)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473)
[M::mem_pestat] mean and std.dev: (194.11, 50.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 580)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 702504 reads in 153.134 CPU sec, 4.869 real sec
[M::mem_process_seqs] Processed 700182 reads in 96.388 CPU sec, 3.057 real sec
sorting block 53, #reads = 1087976 mem = 536877991 peak = 1103368192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291276, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.21, 119.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 453, 1574)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4364)
[M::mem_pestat] mean and std.dev: (498.00, 491.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5759)
[M::mem_pestat] skip orientation RR
[M::process] read 699550 sequences (100000206 bp) in 2.481 CPU sec, 2.617 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291255, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.26, 120.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701410 sequences (100000010 bp) in 1.410 CPU sec, 1.413 real sec...
[M::mem_process_seqs] Processed 699070 reads in 145.773 CPU sec, 4.631 real sec
[M::mem_process_seqs] Processed 699064 reads in 92.511 CPU sec, 2.933 real sec
sorting block 54, #reads = 1088245 mem = 536877317 peak = 1103368192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291108, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (246.92, 119.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 812)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 708604 sequences (100000158 bp) in 2.152 CPU sec, 2.258 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291977, 2, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (214, 419, 2193)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6151)
[M::mem_pestat] mean and std.dev: (534.38, 633.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8130)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (241.83, 116.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 704956 sequences (100000117 bp) in 1.430 CPU sec, 1.427 real sec...
[M::mem_process_seqs] Processed 699550 reads in 148.637 CPU sec, 4.735 real sec
sorting block 55, #reads = 1088539 mem = 536878959 peak = 1103368192
[M::mem_process_seqs] Processed 701410 reads in 94.610 CPU sec, 2.992 real sec
sorting block 56, #reads = 1088292 mem = 536879681 peak = 1103368192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 294085, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 202, 292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586)
[M::mem_pestat] mean and std.dev: (226.74, 108.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 733)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704098 sequences (100000251 bp) in 2.396 CPU sec, 2.509 real sec...
[M::process] read 702658 sequences (100000224 bp) in 1.420 CPU sec, 1.419 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 293469, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 209, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614)
[M::mem_pestat] mean and std.dev: (235.14, 113.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 769)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 351, 1345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3679)
[M::mem_pestat] mean and std.dev: (714.78, 942.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4846)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 708604 reads in 152.708 CPU sec, 4.851 real sec
[M::mem_process_seqs] Processed 704956 reads in 93.596 CPU sec, 2.965 real sec
sorting block 57, #reads = 1088660 mem = 536881486 peak = 1103368192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292528, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 212, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627)
[M::mem_pestat] mean and std.dev: (238.15, 115.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 786)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701566 sequences (100000070 bp) in 2.189 CPU sec, 2.295 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292973, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (242.09, 117.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 804)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703038 sequences (100000061 bp) in 1.370 CPU sec, 1.376 real sec...
[M::mem_process_seqs] Processed 704098 reads in 146.141 CPU sec, 4.654 real sec
[M::mem_process_seqs] Processed 702658 reads in 90.982 CPU sec, 2.880 real sec
sorting block 58, #reads = 1090114 mem = 536879695 peak = 1103368192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292147, 10, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (244.63, 119.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (40, 349, 790)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2290)
[M::mem_pestat] mean and std.dev: (321.00, 300.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3040)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 702506 sequences (100000281 bp) in 2.128 CPU sec, 2.225 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292936, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626)
[M::mem_pestat] mean and std.dev: (239.18, 115.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 784)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 237, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (235.50, 92.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation RR
[M::process] read 701888 sequences (100000053 bp) in 1.380 CPU sec, 1.379 real sec...
[M::mem_process_seqs] Processed 701566 reads in 139.093 CPU sec, 4.426 real sec
[M::mem_process_seqs] Processed 703038 reads in 87.338 CPU sec, 2.763 real sec
sorting block 59, #reads = 1090834 mem = 536880073 peak = 1103368192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292279, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (240.72, 116.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 60, #reads = 1093216 mem = 536881397 peak = 1103368192
[M::process] read 701836 sequences (100000025 bp) in 2.023 CPU sec, 2.122 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292231, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (242.72, 117.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702522 sequences (100000061 bp) in 1.430 CPU sec, 1.422 real sec...
[M::mem_process_seqs] Processed 702506 reads in 138.795 CPU sec, 4.417 real sec
[M::mem_process_seqs] Processed 701888 reads in 87.748 CPU sec, 2.776 real sec
sorting block 61, #reads = 1093058 mem = 536878303 peak = 1103368192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292096, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (243.05, 117.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 302, 1149)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2981)
[M::mem_pestat] mean and std.dev: (515.55, 411.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3897)
[M::mem_pestat] skip orientation RR
[M::process] read 699494 sequences (100000150 bp) in 1.986 CPU sec, 2.072 real sec...
[M::process] read 699256 sequences (100000045 bp) in 1.370 CPU sec, 1.377 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292308, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (240.86, 116.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701836 reads in 147.027 CPU sec, 4.686 real sec
[M::mem_process_seqs] Processed 702522 reads in 96.049 CPU sec, 3.047 real sec
sorting block 62, #reads = 1091782 mem = 536881280 peak = 1103368192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291289, 5, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 340, 2241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6357)
[M::mem_pestat] mean and std.dev: (1049.90, 989.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8415)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (248.15, 120.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 268, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (293.11, 130.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 698418 sequences (100000025 bp) in 2.249 CPU sec, 2.390 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291321, 6, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (248.15, 120.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (277, 365, 621)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1309)
[M::mem_pestat] mean and std.dev: (398.69, 200.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1653)
[M::mem_pestat] skip orientation RR
[M::process] read 698548 sequences (100000196 bp) in 1.350 CPU sec, 1.344 real sec...
[M::mem_process_seqs] Processed 699494 reads in 139.061 CPU sec, 4.419 real sec
[M::mem_process_seqs] Processed 699256 reads in 86.725 CPU sec, 2.751 real sec
sorting block 63, #reads = 1086194 mem = 536883221 peak = 1103368192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290866, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.38, 121.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698194 sequences (100000221 bp) in 2.177 CPU sec, 2.285 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291288, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (251.28, 122.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702824 sequences (100000047 bp) in 1.380 CPU sec, 1.379 real sec...
[M::mem_process_seqs] Processed 698418 reads in 136.918 CPU sec, 4.351 real sec
sorting block 64, #reads = 1091828 mem = 536878560 peak = 1103368192
[M::mem_process_seqs] Processed 698548 reads in 86.151 CPU sec, 2.734 real sec
sorting block 65, #reads = 1090857 mem = 536881712 peak = 1103368192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290923, 5, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 284, 829)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2191)
[M::mem_pestat] mean and std.dev: (373.90, 357.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2872)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (251.06, 122.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 702984 sequences (100000025 bp) in 1.955 CPU sec, 2.046 real sec...
[M::process] read 702288 sequences (100000249 bp) in 1.350 CPU sec, 1.352 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291963, 10, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626)
[M::mem_pestat] mean and std.dev: (239.47, 116.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 784)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 574, 1386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3814)
[M::mem_pestat] mean and std.dev: (648.30, 515.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5028)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 698194 reads in 145.839 CPU sec, 4.648 real sec
[M::mem_process_seqs] Processed 702824 reads in 95.896 CPU sec, 3.046 real sec
sorting block 66, #reads = 1088843 mem = 536880064 peak = 1103368192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292442, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (240.43, 117.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700994 sequences (100000239 bp) in 2.030 CPU sec, 2.123 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292533, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (242.66, 118.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699940 sequences (100000118 bp) in 1.340 CPU sec, 1.343 real sec...
[M::mem_process_seqs] Processed 702984 reads in 133.158 CPU sec, 4.237 real sec
[M::mem_process_seqs] Processed 702288 reads in 83.862 CPU sec, 2.659 real sec
sorting block 67, #reads = 1092421 mem = 536877411 peak = 1103368192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 292029, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 168, 1011)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2735)
[M::mem_pestat] mean and std.dev: (302.80, 308.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3597)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (246.52, 120.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 699800 sequences (100000219 bp) in 2.348 CPU sec, 2.448 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292226, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (249.95, 122.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 308, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (25, 640)
[M::mem_pestat] mean and std.dev: (291.22, 68.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RR
[M::process] read 701240 sequences (100000294 bp) in 1.380 CPU sec, 1.372 real sec...
[M::mem_process_seqs] Processed 700994 reads in 131.157 CPU sec, 4.165 real sec
[M::mem_process_seqs] Processed 699940 reads in 82.056 CPU sec, 2.590 real sec
sorting block 68, #reads = 1095129 mem = 536877950 peak = 1103368192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291830, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (251.21, 123.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 69, #reads = 1095698 mem = 536880073 peak = 1103368192
[M::process] read 701718 sequences (100000219 bp) in 2.111 CPU sec, 2.190 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292393, 3, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (219, 335, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (262.33, 69.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 683)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (245.65, 120.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 264, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (5, 535)
[M::mem_pestat] mean and std.dev: (250.73, 58.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 641)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 700750 sequences (100000227 bp) in 1.370 CPU sec, 1.369 real sec...
[M::mem_process_seqs] Processed 699800 reads in 130.781 CPU sec, 4.160 real sec
[M::mem_process_seqs] Processed 701240 reads in 82.455 CPU sec, 2.606 real sec
sorting block 70, #reads = 1094425 mem = 536881399 peak = 1103368192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292059, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.60, 117.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699400 sequences (100000199 bp) in 2.071 CPU sec, 2.149 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292013, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (246.68, 120.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 378, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (283.67, 122.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RR
[M::process] read 698728 sequences (100000261 bp) in 1.340 CPU sec, 1.341 real sec...
[M::mem_process_seqs] Processed 701718 reads in 133.478 CPU sec, 4.248 real sec
[M::mem_process_seqs] Processed 700750 reads in 83.951 CPU sec, 2.660 real sec
sorting block 71, #reads = 1095331 mem = 536879443 peak = 1103368192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291170, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (249.48, 122.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699100 sequences (100000008 bp) in 2.084 CPU sec, 2.169 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290978, 2, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 234, 286)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 474)
[M::mem_pestat] mean and std.dev: (219.10, 57.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 568)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (250.59, 122.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 699178 sequences (100000093 bp) in 1.380 CPU sec, 1.373 real sec...
[M::mem_process_seqs] Processed 699400 reads in 133.846 CPU sec, 4.263 real sec
[M::mem_process_seqs] Processed 698728 reads in 83.415 CPU sec, 2.641 real sec
sorting block 72, #reads = 1093172 mem = 536878869 peak = 1103368192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291204, 0, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (125, 260, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (202.00, 101.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.51, 120.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698932 sequences (100000010 bp) in 2.321 CPU sec, 2.386 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291632, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (247.05, 119.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 812)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699062 sequences (100000077 bp) in 1.370 CPU sec, 1.377 real sec...
[M::mem_process_seqs] Processed 699100 reads in 139.255 CPU sec, 4.427 real sec
sorting block 73, #reads = 1093396 mem = 536879228 peak = 1103368192
[M::mem_process_seqs] Processed 699178 reads in 87.436 CPU sec, 2.769 real sec
sorting block 74, #reads = 1091146 mem = 536879788 peak = 1103368192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291529, 4, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.47, 120.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 273, 921)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2399)
[M::mem_pestat] mean and std.dev: (616.50, 718.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3492)
[M::mem_pestat] skip orientation RR
[M::process] read 699232 sequences (100000105 bp) in 2.099 CPU sec, 2.178 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290811, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (246.46, 119.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698946 sequences (100000225 bp) in 1.410 CPU sec, 1.410 real sec...
[M::mem_process_seqs] Processed 698932 reads in 137.062 CPU sec, 4.361 real sec
[M::mem_process_seqs] Processed 699062 reads in 87.013 CPU sec, 2.753 real sec
sorting block 75, #reads = 1092156 mem = 536880042 peak = 1103368192
[M::process] read 706706 sequences (100000017 bp) in 2.079 CPU sec, 2.143 real sec...
[M::process] read 703994 sequences (100000051 bp) in 1.370 CPU sec, 1.369 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 290493, 4, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (246, 552, 1400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3708)
[M::mem_pestat] mean and std.dev: (621.69, 492.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4862)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (245.69, 118.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 804)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698946 reads in 87.988 CPU sec, 2.796 real sec
sorting block 76, #reads = 1088371 mem = 536877075 peak = 1103368192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291090, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (246.23, 119.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 293915, 6, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 312, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (314.69, 169.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 205, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (230.67, 111.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 751)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 701934 sequences (100000047 bp) in 1.658 CPU sec, 1.668 real sec...
[M::mem_process_seqs] Processed 699232 reads in 226.421 CPU sec, 7.216 real sec
[M::mem_process_seqs] Processed 706706 reads in 108.165 CPU sec, 3.454 real sec
sorting block 77, #reads = 1088109 mem = 536877199 peak = 1103368192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292785, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 212, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (238.41, 116.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701436 sequences (100000147 bp) in 2.415 CPU sec, 2.496 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292213, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (243.63, 119.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700164 sequences (100000282 bp) in 1.360 CPU sec, 1.364 real sec...
[M::mem_process_seqs] Processed 703994 reads in 141.654 CPU sec, 4.503 real sec
[M::mem_process_seqs] Processed 701934 reads in 89.112 CPU sec, 2.828 real sec
sorting block 78, #reads = 1091681 mem = 536893965 peak = 1103368192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291938, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (246.43, 120.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700098 sequences (100000122 bp) in 2.342 CPU sec, 2.503 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291414, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (250.78, 123.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699898 sequences (100000070 bp) in 1.340 CPU sec, 1.340 real sec...
[M::mem_process_seqs] Processed 701436 reads in 139.170 CPU sec, 4.419 real sec
[M::mem_process_seqs] Processed 700164 reads in 87.270 CPU sec, 2.766 real sec
sorting block 79, #reads = 1092910 mem = 536879443 peak = 1103368192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291524, 14, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 197, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 431)
[M::mem_pestat] mean and std.dev: (211.25, 58.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 515)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (250.17, 122.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 399, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886)
[M::mem_pestat] mean and std.dev: (289.83, 157.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 1042)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2788)
[M::mem_pestat] mean and std.dev: (648.27, 821.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3936)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 80, #reads = 1092120 mem = 536878671 peak = 1124585472
[M::process] read 698220 sequences (100000177 bp) in 1.984 CPU sec, 2.103 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291649, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (249.27, 121.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697840 sequences (100000213 bp) in 1.370 CPU sec, 1.371 real sec...
[M::mem_process_seqs] Processed 700098 reads in 136.878 CPU sec, 4.355 real sec
[M::mem_process_seqs] Processed 699898 reads in 86.106 CPU sec, 2.730 real sec
sorting block 81, #reads = 1092583 mem = 536876811 peak = 1124585472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 290917, 5, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 193, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484)
[M::mem_pestat] mean and std.dev: (193.07, 70.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 598)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.68, 121.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 206, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503)
[M::mem_pestat] mean and std.dev: (216.70, 54.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 609)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 697432 sequences (100000052 bp) in 2.114 CPU sec, 2.261 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 290645, 9, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 246, 986)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2620)
[M::mem_pestat] mean and std.dev: (562.38, 583.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3437)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.94, 121.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697156 sequences (100000161 bp) in 1.340 CPU sec, 1.339 real sec...
[M::mem_process_seqs] Processed 698220 reads in 137.561 CPU sec, 4.376 real sec
[M::mem_process_seqs] Processed 697840 reads in 87.676 CPU sec, 2.776 real sec
sorting block 82, #reads = 1093102 mem = 536879432 peak = 1124585472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290236, 8, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.77, 120.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697022 sequences (100000080 bp) in 2.256 CPU sec, 2.350 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290679, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.29, 118.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 298, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696)
[M::mem_pestat] mean and std.dev: (287.44, 119.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RR
[M::process] read 697164 sequences (100000242 bp) in 1.320 CPU sec, 1.321 real sec...
[M::mem_process_seqs] Processed 697432 reads in 136.538 CPU sec, 4.340 real sec
[M::mem_process_seqs] Processed 697156 reads in 85.392 CPU sec, 2.704 real sec
sorting block 83, #reads = 1090348 mem = 536880281 peak = 1124585472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291114, 2, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 185, 1043)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2859)
[M::mem_pestat] mean and std.dev: (473.62, 599.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3767)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (251.10, 118.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 696942 sequences (100000087 bp) in 2.323 CPU sec, 2.456 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290220, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (249.52, 117.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696548 sequences (100000143 bp) in 1.300 CPU sec, 1.302 real sec...
[M::mem_process_seqs] Processed 697022 reads in 133.708 CPU sec, 4.246 real sec
sorting block 84, #reads = 1090985 mem = 536879366 peak = 1124585472
[M::mem_process_seqs] Processed 697164 reads in 84.383 CPU sec, 2.671 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290315, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 227, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (249.89, 117.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 85, #reads = 1091699 mem = 536878846 peak = 1124585472
[M::process] read 696566 sequences (100000083 bp) in 2.280 CPU sec, 2.397 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290207, 6, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (251.02, 118.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 564, 945)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2417)
[M::mem_pestat] mean and std.dev: (581.88, 384.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3153)
[M::mem_pestat] skip orientation RR
[M::process] read 697038 sequences (100000160 bp) in 1.330 CPU sec, 1.331 real sec...
[M::mem_process_seqs] Processed 696942 reads in 133.482 CPU sec, 4.240 real sec
[M::mem_process_seqs] Processed 696548 reads in 84.184 CPU sec, 2.663 real sec
[M::process] read 4402 sequences (628723 bp) in 0.436 CPU sec, 0.442 real sec...
sorting block 86, #reads = 1091510 mem = 536878596 peak = 1124585472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290264, 7, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 354, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (267.70, 110.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (251.21, 118.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 1835, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 218, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (243.22, 115.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 800)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290435, 12, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (251.14, 119.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 481, 632)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1492)
[M::mem_pestat] mean and std.dev: (409.25, 208.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1922)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 696566 reads in 131.034 CPU sec, 4.250 real sec
[M::mem_process_seqs] Processed 4402 reads in 71.724 CPU sec, 2.312 real sec
[M::mem_process_seqs] Processed 697038 reads in 83.983 CPU sec, 2.728 real sec
sorting block 87, #reads = 1091799 mem = 536879551 peak = 1124585472
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 96451370
overall: 70090715136 mem 11482.842 user 399.550 sys 1050.682 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1047_JG-T1-TDNA-1_A23MHWWLT4_1.L001\tPU:L001\tSM:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tLB:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0003.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0003.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 1050.724 sec; CPU: 11882.399 sec
sorting block 88, #reads = 804660 mem = 396287461 peak = 1126273024
uniq header count 1
merging 89 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 5150610327
output reads: 96943585
bam_mem_sort: 89 calls 33.524 user 0.271 sys 34.031 real
bam_write: 89 calls 63.826 user 2.191 sys 66.461 real
execute: 1 calls 18.248 user 1.411 sys 1078.098 real
merge_files: 1 calls 18.236 user 1.383 sys 26.352 real
parse_chunk: 1420 calls 44.050 user 12.463 sys 57.556 real
read_chunk: 3835 calls 34.906 user 32.774 sys 338.239 real
sort_block: 1 calls 27.777 user 32.166 sys 327.303 real
write_chunk: 2413 calls 17.850 user 1.187 sys 25.544 real
overall: 2990694400 mem 1021.580 user 58.784 sys 1078.120 real
ls: cannot access '*.cram': No such file or directory