Detecting adapter sequence for read1...
>Illumina TruSeq Adapter Read 1
AGATCGGAAGAGCACACGTCTGAACTCCAGTCA

Detecting adapter sequence for read2...
>Illumina TruSeq Adapter Read 2
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT

Read1 before filtering:
total reads: 133580701
total bases: 20170685851
Q20 bases: 19625284091(97.2961%)
Q30 bases: 18594330544(92.1849%)
Q40 bases: 18594330544(92.1849%)

Read2 before filtering:
total reads: 133580701
total bases: 20170685851
Q20 bases: 19118381600(94.783%)
Q30 bases: 17391067804(86.2195%)
Q40 bases: 17391067804(86.2195%)

Read1 after filtering:
total reads: 130584467
total bases: 19116237422
Q20 bases: 18694358478(97.7931%)
Q30 bases: 17791656001(93.0709%)
Q40 bases: 17791656001(93.0709%)

Read2 after filtering:
total reads: 130584467
total bases: 19117654397
Q20 bases: 18323618118(95.8466%)
Q30 bases: 16815033618(87.9555%)
Q40 bases: 16815033618(87.9555%)

Filtering result:
reads passed filter: 261168934
reads failed due to low quality: 5954724
reads failed due to too many N: 30976
reads failed due to too short: 6768
reads with adapter trimmed: 32702618
bases trimmed due to adapters: 1207542206

Duplication rate: 13.1194%

Insert size peak (evaluated by paired-end reads): 265

JSON report: 1029_N5D-T1-TDNA-1_A23MHWWLT4_1.fastp.json
HTML report: 1029_N5D-T1-TDNA-1_A23MHWWLT4_1.fastp.html

fastp --in1 1029_N5D-T1-TDNA-1_A23MHWWLT4_1_S1_L002_R1_001.fastq.gz --in2 1029_N5D-T1-TDNA-1_A23MHWWLT4_1_S1_L002_R2_001.fastq.gz --out1 1029_N5D-T1-TDNA-1_A23MHWWLT4_1/1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz --out2 1029_N5D-T1-TDNA-1_A23MHWWLT4_1/1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz --detect_adapter_for_pe --length_required 15 --json 1029_N5D-T1-TDNA-1_A23MHWWLT4_1.fastp.json --html 1029_N5D-T1-TDNA-1_A23MHWWLT4_1.fastp.html --thread 4 --split_by_lines 400000000 
fastp v1.0.1, time used: 1690 seconds