cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1029_I9B-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1990.048 CPU sec, 103.876 real sec [M::process] read 691706 sequences (100000172 bp) in 1.402 CPU sec, 1.407 real sec... [M::process] read 690732 sequences (100000137 bp) in 1.889 CPU sec, 2.352 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 281210, 9, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 279, 540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1158) [M::mem_pestat] mean and std.dev: (321.13, 158.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 276, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (299.04, 149.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 304, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1164) [M::mem_pestat] mean and std.dev: (338.20, 202.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1482) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689978 sequences (100000132 bp) in 1.981 CPU sec, 2.161 real sec... [M::process] read 689194 sequences (100000196 bp) in 1.801 CPU sec, 1.858 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 281270, 16, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (222, 333, 1144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2988) [M::mem_pestat] mean and std.dev: (577.79, 472.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3910) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 282, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848) [M::mem_pestat] mean and std.dev: (304.75, 152.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1070) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (240, 483, 1727) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4701) [M::mem_pestat] mean and std.dev: (1003.25, 1056.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6188) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 239, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1199) [M::mem_pestat] mean and std.dev: (280.29, 144.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1532) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691706 reads in 230.011 CPU sec, 8.791 real sec [M::mem_process_seqs] Processed 690732 reads in 156.296 CPU sec, 6.448 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 280855, 17, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 313, 1388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3762) [M::mem_pestat] mean and std.dev: (671.50, 793.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4949) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 286, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (308.31, 153.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1081) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (358, 822, 1895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4969) [M::mem_pestat] mean and std.dev: (1228.24, 944.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6506) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 295, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (269.63, 125.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 0, #reads = 1073183 mem = 536888841 peak = 550531072 [M::process] read 688772 sequences (100000279 bp) in 2.873 CPU sec, 3.257 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281306, 9, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (239, 275, 668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1526) [M::mem_pestat] mean and std.dev: (415.16, 340.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1955) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 289, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (310.79, 154.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1083) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (246, 330, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (317.38, 121.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1086) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688492 sequences (100000198 bp) in 2.062 CPU sec, 2.276 real sec... [M::mem_process_seqs] Processed 689978 reads in 223.047 CPU sec, 7.955 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 280715, 6, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 327, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (310.54, 132.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 291, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (312.28, 154.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1092) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 247, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1170) [M::mem_pestat] mean and std.dev: (253.80, 126.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1507) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688508 sequences (100000133 bp) in 2.172 CPU sec, 2.299 real sec... [M::mem_process_seqs] Processed 688772 reads in 131.808 CPU sec, 5.128 real sec sorting block 1, #reads = 1072932 mem = 536878698 peak = 677933056 [M::mem_process_seqs] Processed 689194 reads in 364.286 CPU sec, 14.103 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 280762, 11, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 300, 803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2081) [M::mem_pestat] mean and std.dev: (427.27, 428.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2720) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 293, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (315.35, 156.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (239, 261, 1639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4439) [M::mem_pestat] mean and std.dev: (1045.36, 1430.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6768) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 263, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (279.35, 151.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1171) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687694 sequences (100000068 bp) in 2.892 CPU sec, 3.516 real sec... sorting block 2, #reads = 1073575 mem = 536879297 peak = 844275712 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 280541, 14, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 206, 478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022) [M::mem_pestat] mean and std.dev: (283.00, 188.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1294) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 295, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (316.25, 157.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (130, 571, 867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2341) [M::mem_pestat] mean and std.dev: (543.86, 357.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3078) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 254, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1325) [M::mem_pestat] mean and std.dev: (329.48, 247.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1711) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688492 reads in 251.531 CPU sec, 9.207 real sec [M::mem_process_seqs] Processed 688508 reads in 157.870 CPU sec, 6.078 real sec [M::process] read 687552 sequences (100000209 bp) in 2.829 CPU sec, 3.481 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 280710, 10, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 265, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (311.89, 200.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 296, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (318.48, 158.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (604, 932, 2810) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7222) [M::mem_pestat] mean and std.dev: (1339.20, 1018.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9428) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 260, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012) [M::mem_pestat] mean and std.dev: (244.14, 126.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1294) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 3, #reads = 1073751 mem = 536880693 peak = 844275712 [M::process] read 687404 sequences (100000204 bp) in 2.882 CPU sec, 3.500 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 280797, 14, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 182, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (246.24, 137.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 298, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (319.48, 158.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (89, 530, 592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1598) [M::mem_pestat] mean and std.dev: (469.79, 410.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2113) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (280, 370, 578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1174) [M::mem_pestat] mean and std.dev: (432.95, 237.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1472) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687694 reads in 227.845 CPU sec, 8.380 real sec [M::process] read 687280 sequences (100000106 bp) in 2.117 CPU sec, 2.393 real sec... [M::mem_process_seqs] Processed 687552 reads in 159.082 CPU sec, 6.409 real sec sorting block 4, #reads = 1072785 mem = 536883643 peak = 844275712 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 280364, 14, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 300, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (279.79, 116.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 299, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (320.48, 158.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (621, 1117, 2146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5196) [M::mem_pestat] mean and std.dev: (1372.50, 739.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6721) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (293, 328, 895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2099) [M::mem_pestat] mean and std.dev: (412.50, 299.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2701) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686806 sequences (100000019 bp) in 3.044 CPU sec, 3.878 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 280252, 17, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 281, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (291.66, 142.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 908) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 300, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (321.47, 159.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (221, 573, 2378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6692) [M::mem_pestat] mean and std.dev: (996.06, 979.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8849) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 266, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1106) [M::mem_pestat] mean and std.dev: (283.36, 131.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1399) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687404 reads in 228.172 CPU sec, 8.447 real sec [M::process] read 686622 sequences (100000091 bp) in 2.515 CPU sec, 2.737 real sec... [M::mem_process_seqs] Processed 687280 reads in 159.891 CPU sec, 6.370 real sec sorting block 5, #reads = 1072141 mem = 536881284 peak = 844275712 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 279930, 5, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 227, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (264.70, 134.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 302, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (322.98, 159.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 316, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1049) [M::mem_pestat] mean and std.dev: (332.46, 196.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1335) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686230 sequences (100000239 bp) in 3.056 CPU sec, 3.775 real sec... sorting block 6, #reads = 1073032 mem = 536881369 peak = 844275712 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 279746, 12, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 356, 718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1688) [M::mem_pestat] mean and std.dev: (384.80, 195.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2173) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 302, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (324.12, 160.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (286, 502, 1404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3640) [M::mem_pestat] mean and std.dev: (561.17, 517.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4758) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (308, 427, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977) [M::mem_pestat] mean and std.dev: (420.94, 178.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1200) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686806 reads in 227.693 CPU sec, 8.160 real sec [M::process] read 686696 sequences (100000090 bp) in 2.619 CPU sec, 3.131 real sec... [M::mem_process_seqs] Processed 686622 reads in 160.504 CPU sec, 6.314 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 280299, 5, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 315, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (290.60, 129.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 303, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (324.83, 160.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 339, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948) [M::mem_pestat] mean and std.dev: (357.21, 173.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1194) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 7, #reads = 1073004 mem = 536877268 peak = 844275712 [M::process] read 686170 sequences (100000257 bp) in 3.601 CPU sec, 4.326 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 279850, 13, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 358, 714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1786) [M::mem_pestat] mean and std.dev: (421.83, 301.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2322) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 302, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (323.04, 159.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (293, 1037, 3024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8486) [M::mem_pestat] mean and std.dev: (1235.46, 1167.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11217) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 404, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1155) [M::mem_pestat] mean and std.dev: (378.28, 206.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1467) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686230 reads in 228.326 CPU sec, 7.973 real sec [M::mem_process_seqs] Processed 686696 reads in 159.806 CPU sec, 5.903 real sec [M::process] read 686162 sequences (100000294 bp) in 2.872 CPU sec, 3.399 real sec... sorting block 8, #reads = 1071823 mem = 536881063 peak = 844275712 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 279462, 12, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 291, 552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1320) [M::mem_pestat] mean and std.dev: (304.74, 198.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1704) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 302, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (324.05, 160.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (476, 476, 602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (224, 854) [M::mem_pestat] mean and std.dev: (520.45, 103.66) [M::mem_pestat] low and high boundaries for proper pairs: (98, 980) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 510, 922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2306) [M::mem_pestat] mean and std.dev: (572.85, 342.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2998) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686596 sequences (100000152 bp) in 3.292 CPU sec, 3.960 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 279541, 26, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 289, 847) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2175) [M::mem_pestat] mean and std.dev: (519.26, 485.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2839) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 303, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (324.14, 159.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (479, 629, 703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (31, 1151) [M::mem_pestat] mean and std.dev: (542.33, 170.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1375) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 370, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1173) [M::mem_pestat] mean and std.dev: (366.94, 220.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1504) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686170 reads in 228.603 CPU sec, 8.170 real sec [M::mem_process_seqs] Processed 686162 reads in 159.546 CPU sec, 6.073 real sec [M::process] read 686626 sequences (100000219 bp) in 3.180 CPU sec, 3.687 real sec... sorting block 9, #reads = 1072428 mem = 536877690 peak = 844275712 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 279836, 13, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 299, 542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1320) [M::mem_pestat] mean and std.dev: (361.68, 255.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 300, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (321.85, 159.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (54, 1681, 1681) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4935) [M::mem_pestat] mean and std.dev: (1071.17, 998.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6562) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 313, 609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1445) [M::mem_pestat] mean and std.dev: (286.31, 158.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1863) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686590 sequences (100000119 bp) in 3.333 CPU sec, 3.773 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 280145, 11, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (256, 461, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060) [M::mem_pestat] mean and std.dev: (390.45, 182.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1328) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 299, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (320.92, 158.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (388, 388, 1599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4021) [M::mem_pestat] mean and std.dev: (761.18, 743.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5232) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 279, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (274.04, 106.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686596 reads in 232.288 CPU sec, 8.204 real sec sorting block 10, #reads = 1071882 mem = 536882502 peak = 844275712 [M::mem_process_seqs] Processed 686626 reads in 162.783 CPU sec, 5.852 real sec [M::process] read 686676 sequences (100000159 bp) in 3.265 CPU sec, 3.663 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 279883, 17, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 253, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956) [M::mem_pestat] mean and std.dev: (319.00, 158.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1200) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 297, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (319.26, 157.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (62, 103, 1022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2942) [M::mem_pestat] mean and std.dev: (476.12, 649.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3902) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 463, 552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1214) [M::mem_pestat] mean and std.dev: (439.05, 264.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1545) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 11, #reads = 1071760 mem = 536877780 peak = 844275712 [M::process] read 686744 sequences (100000285 bp) in 3.356 CPU sec, 3.614 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 279948, 13, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 236, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987) [M::mem_pestat] mean and std.dev: (247.40, 171.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1269) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 298, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (319.70, 157.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (154, 906, 3721) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10855) [M::mem_pestat] mean and std.dev: (1698.38, 1937.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14422) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 278, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (286.82, 124.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1072) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686590 reads in 236.517 CPU sec, 7.926 real sec [M::mem_process_seqs] Processed 686676 reads in 164.239 CPU sec, 5.327 real sec [M::process] read 686522 sequences (100000073 bp) in 3.324 CPU sec, 3.550 real sec... sorting block 12, #reads = 1071812 mem = 536881562 peak = 844275712 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 280012, 10, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 308, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1137) [M::mem_pestat] mean and std.dev: (344.81, 208.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1443) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 297, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (319.39, 157.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (226, 986, 1787) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4909) [M::mem_pestat] mean and std.dev: (949.80, 759.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6470) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 260, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1070) [M::mem_pestat] mean and std.dev: (332.83, 229.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1358) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686206 sequences (100000243 bp) in 2.879 CPU sec, 3.137 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 279299, 11, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (331, 1437, 3173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8857) [M::mem_pestat] mean and std.dev: (2096.75, 2314.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 300, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (321.67, 158.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 146, 916) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2702) [M::mem_pestat] mean and std.dev: (427.55, 461.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3595) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 328, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1119) [M::mem_pestat] mean and std.dev: (319.52, 181.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1430) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686744 reads in 237.708 CPU sec, 7.705 real sec [M::process] read 685856 sequences (100000124 bp) in 2.470 CPU sec, 2.528 real sec... [M::mem_process_seqs] Processed 686522 reads in 167.187 CPU sec, 5.397 real sec sorting block 13, #reads = 1072693 mem = 536883599 peak = 844275712 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 279475, 12, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 351, 649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1585) [M::mem_pestat] mean and std.dev: (333.25, 192.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2053) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 301, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (322.38, 158.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1123) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (64, 347, 1122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3238) [M::mem_pestat] mean and std.dev: (371.36, 391.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4296) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 328, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (342.18, 162.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685788 sequences (100000041 bp) in 3.192 CPU sec, 3.437 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 279795, 16, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 263, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (273.59, 146.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1175) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 300, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (321.57, 157.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (207, 489, 694) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1668) [M::mem_pestat] mean and std.dev: (458.73, 318.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2155) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 234, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (239.68, 120.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686024 sequences (100000293 bp) in 2.246 CPU sec, 2.263 real sec... [M::mem_process_seqs] Processed 686206 reads in 231.759 CPU sec, 7.521 real sec sorting block 14, #reads = 1070004 mem = 536880582 peak = 844275712 [M::mem_process_seqs] Processed 685856 reads in 158.452 CPU sec, 5.116 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 279295, 5, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 319, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (297.15, 131.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1190) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 302, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (322.79, 158.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 271, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (242.91, 61.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 15, #reads = 1072004 mem = 536884607 peak = 844275712 [M::process] read 684850 sequences (100000012 bp) in 3.401 CPU sec, 3.648 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 279377, 15, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (246, 435, 1022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2574) [M::mem_pestat] mean and std.dev: (493.62, 415.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3350) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 301, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (321.90, 157.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (719, 880, 3450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8912) [M::mem_pestat] mean and std.dev: (1795.53, 1422.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11643) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 372, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006) [M::mem_pestat] mean and std.dev: (330.24, 156.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1277) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685082 sequences (100000187 bp) in 2.196 CPU sec, 2.204 real sec... [M::mem_process_seqs] Processed 685788 reads in 226.150 CPU sec, 7.280 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 279115, 16, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 249, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (252.20, 96.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1014) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 307, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (327.12, 159.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (160, 1391, 1984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5632) [M::mem_pestat] mean and std.dev: (1693.81, 1641.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8261) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 284, 761) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1993) [M::mem_pestat] mean and std.dev: (432.89, 460.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2609) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685272 sequences (100000239 bp) in 2.558 CPU sec, 2.578 real sec... [M::mem_process_seqs] Processed 684850 reads in 133.615 CPU sec, 4.453 real sec sorting block 16, #reads = 1071635 mem = 536876814 peak = 844275712 [M::mem_process_seqs] Processed 686024 reads in 337.134 CPU sec, 11.054 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 278992, 6, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 277, 599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1445) [M::mem_pestat] mean and std.dev: (337.55, 196.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1868) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 306, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (325.14, 157.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 282, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (244.32, 108.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 17, #reads = 1071488 mem = 536878876 peak = 844275712 [M::process] read 685048 sequences (100000220 bp) in 3.142 CPU sec, 3.326 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 279448, 13, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 306, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (279.30, 109.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 304, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (323.93, 157.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (248, 615, 1265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3299) [M::mem_pestat] mean and std.dev: (737.15, 616.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4316) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 392, 1005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2617) [M::mem_pestat] mean and std.dev: (444.41, 381.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3423) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685082 reads in 255.623 CPU sec, 8.205 real sec [M::process] read 684724 sequences (100000000 bp) in 2.444 CPU sec, 2.484 real sec... [M::mem_process_seqs] Processed 685272 reads in 157.757 CPU sec, 5.040 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 279331, 12, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 335, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1111) [M::mem_pestat] mean and std.dev: (314.88, 152.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 305, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (323.70, 156.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 449, 891) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2627) [M::mem_pestat] mean and std.dev: (535.17, 583.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3495) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (259, 637, 790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1852) [M::mem_pestat] mean and std.dev: (497.35, 277.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2383) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 18, #reads = 1070807 mem = 536878015 peak = 844275712 [M::process] read 684634 sequences (100000300 bp) in 3.259 CPU sec, 3.543 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 279485, 14, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 288, 750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1820) [M::mem_pestat] mean and std.dev: (391.76, 281.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 304, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (323.65, 156.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (71, 1483, 2141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6281) [M::mem_pestat] mean and std.dev: (921.17, 842.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8351) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 391, 691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1611) [M::mem_pestat] mean and std.dev: (358.80, 178.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2071) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684836 sequences (100000181 bp) in 2.071 CPU sec, 2.072 real sec... [M::mem_process_seqs] Processed 685048 reads in 223.404 CPU sec, 7.174 real sec [M::mem_process_seqs] Processed 684724 reads in 150.391 CPU sec, 4.813 real sec sorting block 19, #reads = 1071449 mem = 536882703 peak = 844275712 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 279430, 18, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 244, 1349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3669) [M::mem_pestat] mean and std.dev: (755.56, 843.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 307, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (325.74, 157.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (366, 819, 993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2247) [M::mem_pestat] mean and std.dev: (820.89, 502.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2874) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 282, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849) [M::mem_pestat] mean and std.dev: (313.53, 123.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684418 sequences (100000022 bp) in 3.346 CPU sec, 3.530 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 279555, 18, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 313, 727) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1875) [M::mem_pestat] mean and std.dev: (471.72, 402.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 306, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (325.46, 157.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (146, 552, 1213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3347) [M::mem_pestat] mean and std.dev: (617.93, 504.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4414) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 270, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013) [M::mem_pestat] mean and std.dev: (310.49, 143.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1284) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684066 sequences (100000084 bp) in 2.339 CPU sec, 2.336 real sec... [M::mem_process_seqs] Processed 684634 reads in 214.707 CPU sec, 6.840 real sec [M::mem_process_seqs] Processed 684836 reads in 145.463 CPU sec, 4.634 real sec sorting block 20, #reads = 1072557 mem = 536879715 peak = 844275712 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 279075, 8, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 446, 569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1261) [M::mem_pestat] mean and std.dev: (358.11, 173.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 308, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (327.35, 158.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 265, 647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1583) [M::mem_pestat] mean and std.dev: (383.74, 325.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2051) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683770 sequences (100000018 bp) in 3.330 CPU sec, 3.514 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 279286, 10, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 275, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1232) [M::mem_pestat] mean and std.dev: (276.43, 192.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 313, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (331.81, 160.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1143) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (133, 907, 1425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4009) [M::mem_pestat] mean and std.dev: (875.11, 902.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5301) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (290, 645, 3011) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8453) [M::mem_pestat] mean and std.dev: (1759.98, 2032.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11174) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684340 sequences (100000204 bp) in 2.309 CPU sec, 2.311 real sec... [M::mem_process_seqs] Processed 684418 reads in 205.648 CPU sec, 6.536 real sec sorting block 21, #reads = 1071465 mem = 536879456 peak = 844275712 [M::mem_process_seqs] Processed 684066 reads in 139.055 CPU sec, 4.421 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 279194, 6, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 309, 1126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2914) [M::mem_pestat] mean and std.dev: (615.29, 614.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 313, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (332.58, 161.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 512, 1119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2937) [M::mem_pestat] mean and std.dev: (690.10, 580.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3846) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 22, #reads = 1071197 mem = 536878496 peak = 844275712 [M::process] read 690288 sequences (100000220 bp) in 3.290 CPU sec, 3.540 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 279364, 22, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 273, 841) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2133) [M::mem_pestat] mean and std.dev: (582.88, 495.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2779) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 311, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (330.03, 160.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (390, 630, 1081) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2463) [M::mem_pestat] mean and std.dev: (526.89, 386.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3154) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 313, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1120) [M::mem_pestat] mean and std.dev: (323.11, 196.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1443) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683770 reads in 200.948 CPU sec, 6.386 real sec [M::process] read 690024 sequences (100000228 bp) in 2.309 CPU sec, 2.309 real sec... [M::mem_process_seqs] Processed 684340 reads in 140.269 CPU sec, 4.439 real sec sorting block 23, #reads = 1071753 mem = 536877200 peak = 844275712 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 281233, 17, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 284, 569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1181) [M::mem_pestat] mean and std.dev: (281.96, 92.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 283, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (306.85, 154.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1079) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (353, 958, 1400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3494) [M::mem_pestat] mean and std.dev: (893.44, 745.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4541) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 339, 663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1591) [M::mem_pestat] mean and std.dev: (362.91, 285.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2055) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689326 sequences (100000020 bp) in 3.359 CPU sec, 3.587 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 280898, 8, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 219, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (215.21, 90.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 285, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (308.20, 154.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1084) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 262, 696) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1646) [M::mem_pestat] mean and std.dev: (416.03, 373.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2121) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690288 reads in 194.057 CPU sec, 6.139 real sec [M::process] read 689228 sequences (100000079 bp) in 2.290 CPU sec, 2.290 real sec... [M::mem_process_seqs] Processed 690024 reads in 137.607 CPU sec, 4.350 real sec sorting block 24, #reads = 1070732 mem = 536881500 peak = 844275712 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 281311, 4, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 287, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088) [M::mem_pestat] mean and std.dev: (341.37, 212.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1384) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 290, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (312.78, 157.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (314, 407, 2041) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5495) [M::mem_pestat] mean and std.dev: (1103.48, 1219.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7222) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688486 sequences (100000282 bp) in 2.777 CPU sec, 2.926 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 281057, 9, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 327, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1225) [M::mem_pestat] mean and std.dev: (318.82, 225.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 291, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (314.00, 157.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 279, 1007) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2575) [M::mem_pestat] mean and std.dev: (454.88, 372.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3359) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688476 sequences (100000096 bp) in 1.810 CPU sec, 1.804 real sec... [M::mem_process_seqs] Processed 689326 reads in 195.047 CPU sec, 6.211 real sec sorting block 25, #reads = 1074969 mem = 536883905 peak = 844275712 [M::mem_process_seqs] Processed 689228 reads in 137.733 CPU sec, 4.374 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 280636, 23, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (259, 388, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024) [M::mem_pestat] mean and std.dev: (345.94, 172.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1279) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 294, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (316.83, 158.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (346, 552, 1522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3874) [M::mem_pestat] mean and std.dev: (1216.82, 1151.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5822) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 410, 758) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1908) [M::mem_pestat] mean and std.dev: (440.68, 303.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2483) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 26, #reads = 1075191 mem = 536878970 peak = 844275712 [M::process] read 688262 sequences (100000082 bp) in 3.375 CPU sec, 3.626 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 280047, 8, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (262, 581, 1045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2611) [M::mem_pestat] mean and std.dev: (654.65, 568.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3394) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 295, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (318.14, 159.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 294, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (257.00, 69.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687742 sequences (100000190 bp) in 2.260 CPU sec, 2.265 real sec... [M::mem_process_seqs] Processed 688486 reads in 192.627 CPU sec, 6.085 real sec [M::process] read 687502 sequences (100000262 bp) in 2.369 CPU sec, 2.371 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 279825, 14, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 345, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1112) [M::mem_pestat] mean and std.dev: (312.09, 170.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 297, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (318.90, 159.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (578, 1079, 1079) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2081) [M::mem_pestat] mean and std.dev: (860.00, 282.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2582) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 363, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (307.22, 126.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1064) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688262 reads in 114.540 CPU sec, 3.935 real sec sorting block 27, #reads = 1074178 mem = 536887210 peak = 844275712 [M::mem_process_seqs] Processed 688476 reads in 276.550 CPU sec, 9.066 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 280528, 12, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 201, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (196.62, 88.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 298, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (319.93, 159.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (435, 707, 9570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 27840) [M::mem_pestat] mean and std.dev: (2820.83, 3905.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 36975) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 368, 1110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2954) [M::mem_pestat] mean and std.dev: (498.52, 469.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3876) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 28, #reads = 1073815 mem = 536878615 peak = 851726336 [M::process] read 687368 sequences (100000093 bp) in 3.360 CPU sec, 3.627 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 279888, 12, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 314, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017) [M::mem_pestat] mean and std.dev: (311.67, 150.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1277) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 301, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (322.31, 160.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (682, 697, 1761) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3919) [M::mem_pestat] mean and std.dev: (925.75, 593.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4998) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 311, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (300.76, 144.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687742 reads in 217.966 CPU sec, 6.935 real sec [M::mem_process_seqs] Processed 687502 reads in 136.447 CPU sec, 4.302 real sec [M::process] read 687012 sequences (100000054 bp) in 2.922 CPU sec, 3.080 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 280393, 10, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (238, 330, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (323.49, 133.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 301, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (322.80, 160.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (128, 177, 601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1547) [M::mem_pestat] mean and std.dev: (284.56, 311.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2020) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 314, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (380.09, 233.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1313) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 29, #reads = 1075428 mem = 536878453 peak = 851726336 [M::process] read 686864 sequences (100000063 bp) in 3.289 CPU sec, 3.538 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 279710, 8, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 423, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1317) [M::mem_pestat] mean and std.dev: (350.65, 200.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1696) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 301, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (322.89, 160.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 265, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (266.28, 121.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687368 reads in 181.050 CPU sec, 5.742 real sec [M::process] read 686602 sequences (100000236 bp) in 1.938 CPU sec, 1.982 real sec... [M::mem_process_seqs] Processed 687012 reads in 126.254 CPU sec, 3.977 real sec sorting block 30, #reads = 1074332 mem = 536880217 peak = 851726336 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 280112, 24, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 225, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (233.06, 91.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 303, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (324.52, 161.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (317, 544, 839) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1883) [M::mem_pestat] mean and std.dev: (505.43, 341.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2405) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 437, 1153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2981) [M::mem_pestat] mean and std.dev: (614.46, 559.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3895) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686514 sequences (100000065 bp) in 3.376 CPU sec, 3.613 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 279975, 5, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 446, 1274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3384) [M::mem_pestat] mean and std.dev: (662.83, 700.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 303, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (324.85, 161.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 281, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (263.07, 120.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686864 reads in 173.673 CPU sec, 5.525 real sec [M::mem_process_seqs] Processed 686602 reads in 119.966 CPU sec, 3.790 real sec sorting block 31, #reads = 1072874 mem = 536881659 peak = 851726336 [M::process] read 686328 sequences (100000203 bp) in 2.988 CPU sec, 3.145 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 279866, 17, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 362, 644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1458) [M::mem_pestat] mean and std.dev: (419.41, 290.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1865) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 304, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (325.32, 161.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (73, 248, 3763) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11143) [M::mem_pestat] mean and std.dev: (1398.88, 2252.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14833) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 381, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (322.83, 111.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686226 sequences (100000292 bp) in 2.772 CPU sec, 3.007 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 279863, 17, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 285, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955) [M::mem_pestat] mean and std.dev: (263.24, 161.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1225) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 305, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (327.04, 162.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (169, 418, 784) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2014) [M::mem_pestat] mean and std.dev: (455.56, 291.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2629) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 277, 986) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2660) [M::mem_pestat] mean and std.dev: (388.50, 319.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3497) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686514 reads in 171.943 CPU sec, 5.482 real sec sorting block 32, #reads = 1073510 mem = 536878829 peak = 851726336 [M::mem_process_seqs] Processed 686328 reads in 120.075 CPU sec, 3.786 real sec [M::process] read 686158 sequences (100000018 bp) in 2.556 CPU sec, 2.725 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 280205, 4, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 257, 597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1501) [M::mem_pestat] mean and std.dev: (422.14, 361.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1953) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 305, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (326.70, 162.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 331, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957) [M::mem_pestat] mean and std.dev: (334.11, 229.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1251) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 33, #reads = 1072832 mem = 536880744 peak = 851726336 [M::process] read 686094 sequences (100000064 bp) in 3.313 CPU sec, 3.448 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 280145, 16, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 323, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1172) [M::mem_pestat] mean and std.dev: (343.27, 232.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 304, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (325.81, 161.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (667, 9533, 9533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 27265) [M::mem_pestat] mean and std.dev: (5730.44, 4331.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 36131) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 257, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787) [M::mem_pestat] mean and std.dev: (257.43, 125.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 993) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686226 reads in 173.382 CPU sec, 5.527 real sec [M::process] read 686602 sequences (100000118 bp) in 2.894 CPU sec, 3.010 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 279445, 13, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 273, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (255.16, 127.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1072) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 302, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (324.44, 160.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (379, 773, 1770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4552) [M::mem_pestat] mean and std.dev: (1034.23, 849.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5943) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 438, 707) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1665) [M::mem_pestat] mean and std.dev: (428.91, 318.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2144) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686948 sequences (100000253 bp) in 1.590 CPU sec, 1.594 real sec... [M::mem_process_seqs] Processed 686094 reads in 107.078 CPU sec, 3.376 real sec sorting block 34, #reads = 1072308 mem = 536878857 peak = 851726336 [M::mem_process_seqs] Processed 686158 reads in 250.490 CPU sec, 7.908 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 280019, 14, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 366, 724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1760) [M::mem_pestat] mean and std.dev: (415.80, 271.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2278) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 299, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (321.23, 158.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 416, 1079) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3177) [M::mem_pestat] mean and std.dev: (433.23, 456.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4226) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 281, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (264.30, 117.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 35, #reads = 1071871 mem = 536879836 peak = 914927616 [M::process] read 686950 sequences (100000097 bp) in 3.274 CPU sec, 3.396 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 279932, 23, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 270, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1153) [M::mem_pestat] mean and std.dev: (307.74, 227.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 299, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (322.10, 160.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (61, 641, 1324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3850) [M::mem_pestat] mean and std.dev: (676.41, 666.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5113) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (238, 438, 714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1666) [M::mem_pestat] mean and std.dev: (496.44, 311.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2142) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686602 reads in 203.557 CPU sec, 6.436 real sec [M::mem_process_seqs] Processed 686948 reads in 124.949 CPU sec, 3.938 real sec [M::process] read 686558 sequences (100000115 bp) in 2.919 CPU sec, 3.091 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 280480, 8, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 185, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (197.29, 64.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 299, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (321.70, 160.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (250, 341, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (315.04, 83.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 36, #reads = 1072507 mem = 536880210 peak = 914927616 [M::process] read 686484 sequences (100000014 bp) in 2.750 CPU sec, 2.847 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 279726, 16, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 304, 2059) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5647) [M::mem_pestat] mean and std.dev: (937.59, 1258.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 299, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (322.02, 160.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (144, 1766, 4091) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11985) [M::mem_pestat] mean and std.dev: (2160.94, 2008.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15932) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 295, 536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1218) [M::mem_pestat] mean and std.dev: (359.05, 245.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1559) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686950 reads in 180.066 CPU sec, 5.730 real sec [M::process] read 686534 sequences (100000206 bp) in 1.707 CPU sec, 1.707 real sec... [M::mem_process_seqs] Processed 686558 reads in 124.509 CPU sec, 3.937 real sec sorting block 37, #reads = 1071884 mem = 536879850 peak = 914927616 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 279733, 7, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 268, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (297.42, 163.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 300, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (322.15, 159.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 511, 3318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9530) [M::mem_pestat] mean and std.dev: (1364.04, 1688.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12636) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686364 sequences (100000093 bp) in 3.295 CPU sec, 3.539 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 279742, 7, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 274, 891) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2289) [M::mem_pestat] mean and std.dev: (431.97, 343.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2988) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 299, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (321.55, 159.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 310, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093) [M::mem_pestat] mean and std.dev: (368.27, 244.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1388) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686484 reads in 177.457 CPU sec, 5.602 real sec [M::process] read 686124 sequences (100000247 bp) in 2.059 CPU sec, 2.058 real sec... sorting block 38, #reads = 1070616 mem = 536878880 peak = 914927616 [M::mem_process_seqs] Processed 686534 reads in 124.738 CPU sec, 3.940 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 279909, 8, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (266, 450, 977) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2399) [M::mem_pestat] mean and std.dev: (631.75, 478.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3110) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 301, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (322.69, 159.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 339, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (336.26, 176.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686238 sequences (100000194 bp) in 3.095 CPU sec, 3.305 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 279995, 12, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 204, 552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1360) [M::mem_pestat] mean and std.dev: (256.57, 196.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1764) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 301, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (323.44, 160.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (328, 1891, 1891) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5017) [M::mem_pestat] mean and std.dev: (1373.58, 1330.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6695) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (266, 283, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (325.20, 165.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685676 sequences (100000023 bp) in 1.730 CPU sec, 1.732 real sec... [M::mem_process_seqs] Processed 686364 reads in 184.456 CPU sec, 5.837 real sec sorting block 39, #reads = 1072596 mem = 536879250 peak = 914927616 [M::mem_process_seqs] Processed 686124 reads in 131.899 CPU sec, 4.191 real sec sorting block 40, #reads = 1071629 mem = 536880358 peak = 914927616 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 279829, 18, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 281, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1128) [M::mem_pestat] mean and std.dev: (340.06, 237.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 299, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (321.31, 158.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 321, 957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2775) [M::mem_pestat] mean and std.dev: (475.06, 597.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3684) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 287, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1151) [M::mem_pestat] mean and std.dev: (343.43, 193.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1475) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686082 sequences (100000204 bp) in 3.261 CPU sec, 3.496 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 279315, 11, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 332, 569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1321) [M::mem_pestat] mean and std.dev: (388.98, 262.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1697) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 301, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (322.68, 158.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (845, 845, 1761) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3593) [M::mem_pestat] mean and std.dev: (1047.64, 525.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4509) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 240, 824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2076) [M::mem_pestat] mean and std.dev: (520.03, 501.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2702) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685606 sequences (100000006 bp) in 1.740 CPU sec, 1.745 real sec... [M::mem_process_seqs] Processed 686238 reads in 190.982 CPU sec, 6.064 real sec [M::mem_process_seqs] Processed 685676 reads in 130.091 CPU sec, 4.129 real sec sorting block 41, #reads = 1071347 mem = 536879247 peak = 914927616 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 279796, 22, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 477, 697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1561) [M::mem_pestat] mean and std.dev: (415.25, 257.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1993) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 303, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (324.97, 160.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1136) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (370, 502, 1885) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4915) [M::mem_pestat] mean and std.dev: (909.95, 724.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6430) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 324, 660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1516) [M::mem_pestat] mean and std.dev: (338.79, 170.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1944) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685552 sequences (100000009 bp) in 3.041 CPU sec, 3.188 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 279482, 14, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 248, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (290.00, 157.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1208) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 304, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (325.82, 160.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (107, 271, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (189.73, 116.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 334, 566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1212) [M::mem_pestat] mean and std.dev: (332.46, 176.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1535) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685554 sequences (100000077 bp) in 1.780 CPU sec, 1.781 real sec... [M::mem_process_seqs] Processed 686082 reads in 180.249 CPU sec, 5.716 real sec [M::mem_process_seqs] Processed 685606 reads in 123.129 CPU sec, 3.906 real sec sorting block 42, #reads = 1070584 mem = 536879952 peak = 914927616 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 279263, 10, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 299, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (334.30, 166.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1081) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 306, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (326.55, 160.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (358, 565, 1424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3556) [M::mem_pestat] mean and std.dev: (693.33, 453.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4622) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 388, 620) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1528) [M::mem_pestat] mean and std.dev: (375.68, 233.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1982) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685332 sequences (100000094 bp) in 3.194 CPU sec, 3.383 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 279065, 10, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 373, 1853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5121) [M::mem_pestat] mean and std.dev: (780.49, 874.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 305, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (326.72, 161.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (174, 642, 704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1764) [M::mem_pestat] mean and std.dev: (561.90, 437.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2311) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 310, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1217) [M::mem_pestat] mean and std.dev: (303.21, 189.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1560) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684808 sequences (100000055 bp) in 2.000 CPU sec, 2.007 real sec... [M::mem_process_seqs] Processed 685552 reads in 181.583 CPU sec, 5.750 real sec sorting block 43, #reads = 1070858 mem = 536878919 peak = 914927616 [M::mem_process_seqs] Processed 685554 reads in 127.287 CPU sec, 4.031 real sec sorting block 44, #reads = 1069476 mem = 536880178 peak = 944533504 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 280332, 10, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 204, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (254.23, 121.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 304, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (324.85, 158.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (469, 737, 1068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2266) [M::mem_pestat] mean and std.dev: (520.38, 342.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2865) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 367, 561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1259) [M::mem_pestat] mean and std.dev: (361.74, 184.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1608) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684558 sequences (100000040 bp) in 2.910 CPU sec, 3.020 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 279524, 5, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 423, 601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1401) [M::mem_pestat] mean and std.dev: (400.83, 267.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1801) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 306, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (326.54, 159.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 217, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (237.89, 111.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684610 sequences (100000179 bp) in 1.930 CPU sec, 1.926 real sec... [M::mem_process_seqs] Processed 685332 reads in 179.103 CPU sec, 5.672 real sec [M::mem_process_seqs] Processed 684808 reads in 125.374 CPU sec, 3.978 real sec sorting block 45, #reads = 1071450 mem = 536876631 peak = 944533504 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 278937, 10, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 216, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001) [M::mem_pestat] mean and std.dev: (303.57, 189.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1278) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 307, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (327.70, 159.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (241, 480, 2363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6607) [M::mem_pestat] mean and std.dev: (1157.00, 1215.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8729) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 269, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (23, 538) [M::mem_pestat] mean and std.dev: (264.59, 66.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684366 sequences (100000209 bp) in 2.835 CPU sec, 2.975 real sec... [M::process] read 684054 sequences (100000169 bp) in 1.820 CPU sec, 1.817 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 279552, 15, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 294, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046) [M::mem_pestat] mean and std.dev: (290.94, 184.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1333) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 310, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (330.07, 161.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (378, 758, 978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2178) [M::mem_pestat] mean and std.dev: (730.07, 338.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2778) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 289, 863) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2187) [M::mem_pestat] mean and std.dev: (354.90, 361.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2849) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684558 reads in 179.239 CPU sec, 5.679 real sec [M::mem_process_seqs] Processed 684610 reads in 124.074 CPU sec, 3.941 real sec sorting block 46, #reads = 1071504 mem = 536878232 peak = 944533504 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 279482, 8, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 320, 906) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2296) [M::mem_pestat] mean and std.dev: (447.86, 351.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2991) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 312, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (333.12, 163.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1158) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (258, 467, 1291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3357) [M::mem_pestat] mean and std.dev: (674.58, 600.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4390) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 690468 sequences (100000238 bp) in 3.276 CPU sec, 3.491 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 279316, 9, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 499, 1140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3006) [M::mem_pestat] mean and std.dev: (591.43, 399.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3939) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 313, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (334.19, 164.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1159) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 290, 1274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3368) [M::mem_pestat] mean and std.dev: (709.18, 850.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4415) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684366 reads in 178.461 CPU sec, 5.654 real sec [M::process] read 690362 sequences (100000279 bp) in 1.918 CPU sec, 1.935 real sec... sorting block 47, #reads = 1070201 mem = 536889933 peak = 944533504 [M::mem_process_seqs] Processed 684054 reads in 126.363 CPU sec, 3.991 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 280950, 8, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (248, 418, 852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2060) [M::mem_pestat] mean and std.dev: (529.13, 399.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 283, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (306.20, 153.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1075) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 250, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953) [M::mem_pestat] mean and std.dev: (296.63, 194.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1205) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689712 sequences (100000043 bp) in 2.497 CPU sec, 2.602 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 281009, 17, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 230, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (250.50, 130.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 689234 sequences (100000195 bp) in 1.930 CPU sec, 1.932 real sec... [M::mem_pestat] (25, 50, 75) percentile: (183, 283, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (306.57, 153.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1079) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (607, 1216, 1729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3973) [M::mem_pestat] mean and std.dev: (986.73, 495.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5095) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (278, 419, 1233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3143) [M::mem_pestat] mean and std.dev: (617.16, 471.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4098) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690468 reads in 187.234 CPU sec, 5.960 real sec sorting block 48, #reads = 1070824 mem = 536880478 peak = 944533504 [M::mem_process_seqs] Processed 690362 reads in 127.771 CPU sec, 4.064 real sec sorting block 49, #reads = 1072844 mem = 536878921 peak = 1006477312 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 281043, 12, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 269, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1085) [M::mem_pestat] mean and std.dev: (252.90, 125.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1395) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 287, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (308.77, 153.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1085) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (420, 1624, 3765) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10455) [M::mem_pestat] mean and std.dev: (1993.25, 2227.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13800) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 560, 1808) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4950) [M::mem_pestat] mean and std.dev: (887.79, 910.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6521) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688922 sequences (100000051 bp) in 3.244 CPU sec, 3.396 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 280966, 6, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 249, 691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1767) [M::mem_pestat] mean and std.dev: (412.76, 428.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2305) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 288, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (309.56, 152.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1079) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 407, 1115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2969) [M::mem_pestat] mean and std.dev: (573.87, 567.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3896) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688246 sequences (100000258 bp) in 2.020 CPU sec, 2.024 real sec... [M::mem_process_seqs] Processed 689712 reads in 182.451 CPU sec, 5.783 real sec [M::mem_process_seqs] Processed 689234 reads in 129.101 CPU sec, 4.087 real sec sorting block 50, #reads = 1073170 mem = 536879395 peak = 1006477312 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 281629, 5, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 306, 777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2011) [M::mem_pestat] mean and std.dev: (324.16, 221.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 291, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (312.10, 154.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1092) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (252, 363, 1456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3864) [M::mem_pestat] mean and std.dev: (638.55, 597.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5068) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687916 sequences (100000208 bp) in 3.187 CPU sec, 3.337 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 280719, 15, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 248, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045) [M::mem_pestat] mean and std.dev: (285.75, 136.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1324) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 294, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (314.80, 155.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (889, 1016, 1676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3250) [M::mem_pestat] mean and std.dev: (885.42, 448.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4037) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 361, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (324.17, 136.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687882 sequences (100000104 bp) in 1.720 CPU sec, 1.719 real sec... [M::mem_process_seqs] Processed 688922 reads in 181.894 CPU sec, 5.780 real sec sorting block 51, #reads = 1074331 mem = 536877932 peak = 1006477312 [M::mem_process_seqs] Processed 688246 reads in 127.395 CPU sec, 4.053 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 280302, 15, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 1179, 1179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3113) [M::mem_pestat] mean and std.dev: (814.87, 602.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4080) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 295, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (315.51, 155.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (541, 1896, 7486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 21376) [M::mem_pestat] mean and std.dev: (3640.13, 3192.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 28321) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 289, 541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1259) [M::mem_pestat] mean and std.dev: (297.43, 176.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1618) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687120 sequences (100000126 bp) in 3.273 CPU sec, 3.431 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 280837, 9, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 233, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (244.12, 122.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 295, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (316.17, 155.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 216, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (244.90, 113.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 997) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687916 reads in 187.313 CPU sec, 5.932 real sec [M::process] read 687236 sequences (100000038 bp) in 2.347 CPU sec, 2.348 real sec... sorting block 52, #reads = 1073591 mem = 536881998 peak = 1006477312 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 280816, 20, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 270, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (284.28, 150.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 298, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (318.12, 155.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (404, 575, 1237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2903) [M::mem_pestat] mean and std.dev: (760.90, 504.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3736) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 287, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1271) [M::mem_pestat] mean and std.dev: (326.13, 194.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1641) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686926 sequences (100000080 bp) in 2.246 CPU sec, 2.253 real sec... [M::mem_process_seqs] Processed 687120 reads in 106.455 CPU sec, 3.403 real sec sorting block 53, #reads = 1071186 mem = 536879837 peak = 1006477312 [M::mem_process_seqs] Processed 687882 reads in 261.963 CPU sec, 8.340 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 279706, 10, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 236, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (213.81, 83.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 298, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (317.84, 154.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (490, 827, 3372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9136) [M::mem_pestat] mean and std.dev: (2428.10, 3051.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14634) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 252, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1129) [M::mem_pestat] mean and std.dev: (285.77, 138.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1440) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686678 sequences (100000076 bp) in 2.542 CPU sec, 2.685 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 280265, 29, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 244, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (240.20, 99.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 301, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (319.87, 156.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (422, 490, 1824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4628) [M::mem_pestat] mean and std.dev: (1172.17, 1340.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6533) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 380, 506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1112) [M::mem_pestat] mean and std.dev: (377.25, 203.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1415) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686434 sequences (100000250 bp) in 1.840 CPU sec, 1.832 real sec... [M::mem_process_seqs] Processed 687236 reads in 203.764 CPU sec, 6.475 real sec sorting block 54, #reads = 1072559 mem = 536885989 peak = 1006477312 [M::mem_process_seqs] Processed 686926 reads in 127.545 CPU sec, 4.043 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 280120, 5, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 313, 1269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3395) [M::mem_pestat] mean and std.dev: (654.78, 649.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 301, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (321.41, 157.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1123) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 226, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (250.68, 137.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1176) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 55, #reads = 1071536 mem = 536878151 peak = 1006477312 [M::process] read 686278 sequences (100000111 bp) in 3.125 CPU sec, 3.304 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 280496, 7, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 205, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (192.86, 95.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 304, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (323.40, 158.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 319, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (273.00, 95.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686128 sequences (100000225 bp) in 2.180 CPU sec, 2.180 real sec... [M::mem_process_seqs] Processed 686678 reads in 187.799 CPU sec, 5.953 real sec [M::mem_process_seqs] Processed 686434 reads in 129.409 CPU sec, 4.098 real sec sorting block 56, #reads = 1071330 mem = 536882333 peak = 1006477312 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 280002, 6, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 322, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1165) [M::mem_pestat] mean and std.dev: (332.24, 174.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 304, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (323.57, 158.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 293, 780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1902) [M::mem_pestat] mean and std.dev: (489.77, 397.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2463) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685900 sequences (100000271 bp) in 3.299 CPU sec, 3.528 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 279327, 17, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 265, 894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2380) [M::mem_pestat] mean and std.dev: (423.74, 397.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3123) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 305, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (325.34, 159.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (82, 459, 1595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4621) [M::mem_pestat] mean and std.dev: (706.29, 636.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6134) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 202, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784) [M::mem_pestat] mean and std.dev: (241.00, 111.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686278 reads in 187.025 CPU sec, 5.936 real sec [M::process] read 685988 sequences (100000229 bp) in 2.110 CPU sec, 2.110 real sec... [M::mem_process_seqs] Processed 686128 reads in 139.632 CPU sec, 4.449 real sec sorting block 57, #reads = 1072522 mem = 536880194 peak = 1006477312 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 279879, 10, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 251, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1141) [M::mem_pestat] mean and std.dev: (293.75, 168.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 304, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (324.39, 158.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (707, 2437, 2437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5897) [M::mem_pestat] mean and std.dev: (1743.50, 955.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7627) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 307, 894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2242) [M::mem_pestat] mean and std.dev: (473.23, 310.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2916) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686156 sequences (100000228 bp) in 3.149 CPU sec, 3.432 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 279846, 26, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (267, 301, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 651) [M::mem_pestat] mean and std.dev: (306.35, 135.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 305, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (325.44, 159.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (329, 343, 680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1382) [M::mem_pestat] mean and std.dev: (358.05, 217.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1733) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 415, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1065) [M::mem_pestat] mean and std.dev: (359.33, 160.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1350) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685900 reads in 182.272 CPU sec, 5.789 real sec [M::process] read 686076 sequences (100000232 bp) in 1.901 CPU sec, 1.905 real sec... sorting block 58, #reads = 1072168 mem = 536878237 peak = 1006477312 [M::mem_process_seqs] Processed 685988 reads in 125.904 CPU sec, 3.988 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 279628, 15, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 336, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068) [M::mem_pestat] mean and std.dev: (312.32, 144.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 304, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (325.37, 160.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (509, 2054, 3623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9851) [M::mem_pestat] mean and std.dev: (2075.33, 1870.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12965) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 440, 1183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3115) [M::mem_pestat] mean and std.dev: (622.48, 610.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4081) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686150 sequences (100000055 bp) in 2.919 CPU sec, 3.028 real sec... [M::process] read 686032 sequences (100000165 bp) in 1.800 CPU sec, 1.794 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 278574, 16, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 288, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013) [M::mem_pestat] mean and std.dev: (276.92, 158.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1306) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 303, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (323.81, 158.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (340, 407, 684) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1372) [M::mem_pestat] mean and std.dev: (344.46, 180.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1716) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 553, 1476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3990) [M::mem_pestat] mean and std.dev: (627.43, 560.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5247) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686156 reads in 186.254 CPU sec, 5.921 real sec sorting block 59, #reads = 1071527 mem = 536877897 peak = 1006477312 [M::mem_process_seqs] Processed 686076 reads in 125.142 CPU sec, 3.983 real sec sorting block 60, #reads = 1071669 mem = 536878812 peak = 1006477312 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 279314, 20, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 199, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1244) [M::mem_pestat] mean and std.dev: (275.47, 227.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 301, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (322.50, 158.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1123) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 222, 791) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2343) [M::mem_pestat] mean and std.dev: (287.47, 331.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3119) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 486, 852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2138) [M::mem_pestat] mean and std.dev: (622.15, 541.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2787) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686170 sequences (100000095 bp) in 2.762 CPU sec, 2.967 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 279059, 9, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 167, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (185.15, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 686078 sequences (100000044 bp) in 1.670 CPU sec, 1.668 real sec... [M::mem_pestat] (25, 50, 75) percentile: (195, 300, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (321.66, 157.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (309, 407, 660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1362) [M::mem_pestat] mean and std.dev: (458.10, 242.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1713) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686150 reads in 185.166 CPU sec, 5.901 real sec [M::mem_process_seqs] Processed 686032 reads in 128.253 CPU sec, 4.083 real sec sorting block 61, #reads = 1070590 mem = 536878223 peak = 1006477312 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 280314, 15, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 344, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (239.88, 98.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 300, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (321.87, 158.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (695, 1216, 1599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3407) [M::mem_pestat] mean and std.dev: (914.50, 557.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4311) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 490, 759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1791) [M::mem_pestat] mean and std.dev: (521.08, 389.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2307) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686082 sequences (100000045 bp) in 2.465 CPU sec, 2.555 real sec... [M::process] read 686076 sequences (100000094 bp) in 1.660 CPU sec, 1.661 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 279735, 36, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 274, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (246.89, 103.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 302, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (323.51, 159.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (267, 698, 1337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3477) [M::mem_pestat] mean and std.dev: (769.37, 577.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4547) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 335, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (323.46, 141.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686170 reads in 176.867 CPU sec, 5.631 real sec [M::mem_process_seqs] Processed 686078 reads in 121.046 CPU sec, 3.864 real sec sorting block 62, #reads = 1070605 mem = 536882063 peak = 1006477312 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 279264, 10, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 251, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (239.27, 82.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 299, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (321.27, 158.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (156, 1951, 3448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10032) [M::mem_pestat] mean and std.dev: (1742.70, 1555.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13324) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 232, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (47, 452) [M::mem_pestat] mean and std.dev: (226.65, 65.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685952 sequences (100000125 bp) in 2.786 CPU sec, 2.898 real sec... [M::process] read 685734 sequences (100000027 bp) in 1.650 CPU sec, 1.654 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 279129, 4, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 366, 547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1223) [M::mem_pestat] mean and std.dev: (371.34, 244.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 300, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (321.31, 157.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 385, 692) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1634) [M::mem_pestat] mean and std.dev: (441.91, 299.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2105) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686082 reads in 183.411 CPU sec, 5.831 real sec sorting block 63, #reads = 1071296 mem = 536877303 peak = 1006477312 [M::mem_process_seqs] Processed 686076 reads in 125.436 CPU sec, 4.002 real sec sorting block 64, #reads = 1071284 mem = 536879587 peak = 1006477312 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 279260, 5, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 337, 1274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3352) [M::mem_pestat] mean and std.dev: (794.31, 874.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4391) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 302, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (322.57, 158.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1123) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 303, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (305.42, 166.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685756 sequences (100000176 bp) in 2.546 CPU sec, 2.619 real sec... [M::process] read 685802 sequences (100000169 bp) in 1.660 CPU sec, 1.661 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 279068, 17, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 236, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (289.55, 186.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 303, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (324.09, 159.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (414, 1036, 1434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3474) [M::mem_pestat] mean and std.dev: (1117.35, 912.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4766) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 337, 697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1693) [M::mem_pestat] mean and std.dev: (438.45, 256.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2191) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685952 reads in 183.701 CPU sec, 5.851 real sec [M::mem_process_seqs] Processed 685734 reads in 124.297 CPU sec, 3.970 real sec sorting block 65, #reads = 1071775 mem = 536879424 peak = 1006477312 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 279632, 10, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (222, 267, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1158) [M::mem_pestat] mean and std.dev: (406.54, 268.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 304, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (324.90, 160.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (577, 1377, 2264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5638) [M::mem_pestat] mean and std.dev: (1841.40, 1717.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8711) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 360, 724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1804) [M::mem_pestat] mean and std.dev: (330.56, 216.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2344) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685454 sequences (100000167 bp) in 2.866 CPU sec, 3.083 real sec... [M::process] read 685426 sequences (100000187 bp) in 1.700 CPU sec, 1.705 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 279101, 19, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 405, 703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1727) [M::mem_pestat] mean and std.dev: (532.39, 439.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2291) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 302, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (323.92, 159.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (370, 507, 872) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1876) [M::mem_pestat] mean and std.dev: (510.41, 413.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2378) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 412, 635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1539) [M::mem_pestat] mean and std.dev: (414.14, 281.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1991) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685756 reads in 184.969 CPU sec, 5.882 real sec [M::mem_process_seqs] Processed 685802 reads in 125.249 CPU sec, 3.991 real sec sorting block 66, #reads = 1070535 mem = 536880777 peak = 1006477312 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 279410, 12, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (234, 264, 595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1317) [M::mem_pestat] mean and std.dev: (361.48, 228.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 305, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (326.14, 160.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (397, 525, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (115, 820) [M::mem_pestat] mean and std.dev: (450.60, 106.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 486, 895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2263) [M::mem_pestat] mean and std.dev: (600.46, 507.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2947) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685522 sequences (100000071 bp) in 3.011 CPU sec, 3.234 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 279086, 14, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 322, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1139) [M::mem_pestat] mean and std.dev: (318.86, 160.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 305, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (326.31, 160.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (500, 1123, 1123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2369) [M::mem_pestat] mean and std.dev: (852.64, 376.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2992) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 322, 704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1744) [M::mem_pestat] mean and std.dev: (444.23, 318.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2264) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684906 sequences (100000290 bp) in 1.700 CPU sec, 1.702 real sec... [M::mem_process_seqs] Processed 685454 reads in 179.144 CPU sec, 5.697 real sec sorting block 67, #reads = 1071341 mem = 536881979 peak = 1006477312 [M::mem_process_seqs] Processed 685426 reads in 122.518 CPU sec, 3.908 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 279247, 18, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (260, 619, 905) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2195) [M::mem_pestat] mean and std.dev: (535.90, 284.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2840) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 306, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (326.80, 160.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1138) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (91, 563, 760) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2098) [M::mem_pestat] mean and std.dev: (493.25, 346.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2767) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 425, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1201) [M::mem_pestat] mean and std.dev: (373.42, 182.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1525) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684686 sequences (100000128 bp) in 2.426 CPU sec, 2.513 real sec... [M::process] read 684646 sequences (100000264 bp) in 1.683 CPU sec, 1.690 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 279805, 7, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 231, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (227.16, 98.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 308, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (328.22, 160.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (245, 278, 700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1610) [M::mem_pestat] mean and std.dev: (430.00, 315.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2065) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685522 reads in 176.341 CPU sec, 5.613 real sec sorting block 68, #reads = 1070347 mem = 536879109 peak = 1006477312 [M::mem_process_seqs] Processed 684906 reads in 118.515 CPU sec, 3.783 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 279631, 7, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 301, 734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1886) [M::mem_pestat] mean and std.dev: (443.10, 329.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 308, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (329.31, 161.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 423, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1192) [M::mem_pestat] mean and std.dev: (357.82, 160.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1525) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 69, #reads = 1072201 mem = 536882375 peak = 1006477312 [M::process] read 684366 sequences (100000082 bp) in 2.692 CPU sec, 2.897 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 279854, 16, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 268, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1129) [M::mem_pestat] mean and std.dev: (341.04, 229.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1430) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 311, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (331.59, 162.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (163, 349, 590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1444) [M::mem_pestat] mean and std.dev: (410.81, 263.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1871) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 302, 548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1216) [M::mem_pestat] mean and std.dev: (282.36, 149.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1550) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684278 sequences (100000187 bp) in 1.650 CPU sec, 1.647 real sec... [M::mem_process_seqs] Processed 684686 reads in 168.158 CPU sec, 5.353 real sec [M::mem_process_seqs] Processed 684646 reads in 114.586 CPU sec, 3.638 real sec sorting block 70, #reads = 1071282 mem = 536877838 peak = 1006477312 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 279250, 14, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 482, 603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1343) [M::mem_pestat] mean and std.dev: (457.38, 258.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 312, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (331.68, 162.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (174, 174, 1618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4506) [M::mem_pestat] mean and std.dev: (784.54, 870.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5950) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 362, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1220) [M::mem_pestat] mean and std.dev: (405.00, 266.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1580) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684592 sequences (100000135 bp) in 2.963 CPU sec, 3.066 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 279334, 13, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 288, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (311.37, 131.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 312, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (331.41, 161.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (349, 657, 1147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2743) [M::mem_pestat] mean and std.dev: (675.42, 318.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3541) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (255, 378, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (360.72, 145.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691238 sequences (100000016 bp) in 1.720 CPU sec, 1.720 real sec... [M::mem_process_seqs] Processed 684366 reads in 166.601 CPU sec, 5.283 real sec [M::mem_process_seqs] Processed 684278 reads in 113.470 CPU sec, 3.608 real sec sorting block 71, #reads = 1071259 mem = 536880540 peak = 1006477312 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 279432, 18, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 264, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1142) [M::mem_pestat] mean and std.dev: (351.03, 237.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 310, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (330.13, 161.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (445, 525, 1418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3364) [M::mem_pestat] mean and std.dev: (863.81, 780.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4337) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (262, 355, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (302.66, 102.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 990) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 690518 sequences (100000026 bp) in 2.559 CPU sec, 2.626 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 281433, 19, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (316, 418, 830) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1858) [M::mem_pestat] mean and std.dev: (420.14, 233.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2372) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 280, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (305.32, 155.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1081) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (616, 799, 1579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3505) [M::mem_pestat] mean and std.dev: (923.22, 575.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4468) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 229, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (281.39, 129.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689918 sequences (100000135 bp) in 2.190 CPU sec, 2.192 real sec... [M::mem_process_seqs] Processed 684592 reads in 169.536 CPU sec, 5.366 real sec sorting block 72, #reads = 1069986 mem = 536878865 peak = 1006477312 [M::mem_process_seqs] Processed 691238 reads in 114.494 CPU sec, 3.630 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 280953, 6, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (340, 602, 1032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2416) [M::mem_pestat] mean and std.dev: (626.81, 480.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3108) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 284, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (309.53, 157.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 325, 1126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2926) [M::mem_pestat] mean and std.dev: (562.56, 647.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3826) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 73, #reads = 1072703 mem = 536879332 peak = 1017786368 [M::process] read 689450 sequences (100000274 bp) in 2.945 CPU sec, 3.018 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 281375, 10, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 163, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (184.81, 75.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 287, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (311.66, 158.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (129, 497, 808) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2166) [M::mem_pestat] mean and std.dev: (497.78, 380.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2845) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 306, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (304.78, 130.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689144 sequences (100000080 bp) in 1.680 CPU sec, 1.684 real sec... [M::mem_process_seqs] Processed 690518 reads in 166.713 CPU sec, 5.302 real sec [M::mem_process_seqs] Processed 689918 reads in 113.190 CPU sec, 3.588 real sec sorting block 74, #reads = 1074909 mem = 536876475 peak = 1017786368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 280594, 11, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 231, 920) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2434) [M::mem_pestat] mean and std.dev: (434.32, 440.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3191) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 289, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (313.92, 159.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (233, 552, 1557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4205) [M::mem_pestat] mean and std.dev: (814.64, 686.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5529) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (270, 330, 659) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1437) [M::mem_pestat] mean and std.dev: (441.62, 296.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1826) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688750 sequences (100000093 bp) in 3.287 CPU sec, 3.518 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 280693, 6, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 311, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (271.59, 104.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1170) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 291, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (315.08, 159.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (142, 243, 581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1459) [M::mem_pestat] mean and std.dev: (255.54, 162.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1898) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689450 reads in 167.283 CPU sec, 5.309 real sec [M::process] read 688298 sequences (100000022 bp) in 1.768 CPU sec, 1.776 real sec... [M::mem_process_seqs] Processed 689144 reads in 115.287 CPU sec, 3.649 real sec sorting block 75, #reads = 1075128 mem = 536878168 peak = 1017786368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 280518, 18, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (248, 333, 674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1526) [M::mem_pestat] mean and std.dev: (446.76, 306.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1952) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 292, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (315.92, 159.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (60, 654, 1023) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2949) [M::mem_pestat] mean and std.dev: (669.06, 745.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3912) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 346, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (312.00, 106.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688630 sequences (100000081 bp) in 3.278 CPU sec, 3.474 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 280239, 19, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 204, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (209.00, 70.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 293, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (317.24, 159.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (831, 1123, 2738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6552) [M::mem_pestat] mean and std.dev: (1035.80, 661.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8459) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 249, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (243.67, 88.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688750 reads in 170.989 CPU sec, 5.431 real sec [M::mem_process_seqs] Processed 688298 reads in 116.028 CPU sec, 3.666 real sec [M::process] read 687768 sequences (100000272 bp) in 2.301 CPU sec, 2.343 real sec... sorting block 76, #reads = 1075191 mem = 536886056 peak = 1017786368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 280873, 5, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 265, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1285) [M::mem_pestat] mean and std.dev: (336.17, 256.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 294, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (318.23, 160.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (296, 446, 891) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2081) [M::mem_pestat] mean and std.dev: (564.34, 465.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2676) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688008 sequences (100000041 bp) in 2.981 CPU sec, 3.122 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 280300, 6, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 273, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1152) [M::mem_pestat] mean and std.dev: (331.15, 253.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 298, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (320.34, 160.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 296, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1256) [M::mem_pestat] mean and std.dev: (364.39, 245.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1619) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688630 reads in 179.926 CPU sec, 5.710 real sec sorting block 77, #reads = 1073846 mem = 536879474 peak = 1017786368 [M::process] read 687478 sequences (100000178 bp) in 2.259 CPU sec, 2.274 real sec... [M::mem_process_seqs] Processed 687768 reads in 126.342 CPU sec, 3.995 real sec sorting block 78, #reads = 1074745 mem = 536879211 peak = 1095970816 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 279800, 12, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 213, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (278.14, 219.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1155) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 297, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (319.98, 159.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1138) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (119, 532, 1186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3320) [M::mem_pestat] mean and std.dev: (703.83, 658.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4387) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 317, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1241) [M::mem_pestat] mean and std.dev: (371.48, 253.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1598) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687612 sequences (100000074 bp) in 2.564 CPU sec, 2.668 real sec... [M::process] read 688134 sequences (100000180 bp) in 1.650 CPU sec, 1.646 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 280076, 5, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 316, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042) [M::mem_pestat] mean and std.dev: (344.10, 193.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1331) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 300, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (321.83, 159.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1136) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 354, 555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1213) [M::mem_pestat] mean and std.dev: (343.90, 176.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1542) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688008 reads in 189.520 CPU sec, 6.046 real sec [M::mem_process_seqs] Processed 687478 reads in 129.335 CPU sec, 4.126 real sec sorting block 79, #reads = 1073812 mem = 536881086 peak = 1095970816 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 280410, 11, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 262, 1140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3094) [M::mem_pestat] mean and std.dev: (481.12, 586.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4071) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 298, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (320.53, 159.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (128, 607, 1279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3581) [M::mem_pestat] mean and std.dev: (533.70, 441.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4732) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (258, 348, 1719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4641) [M::mem_pestat] mean and std.dev: (805.70, 844.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6102) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687374 sequences (100000174 bp) in 2.712 CPU sec, 2.797 real sec... [M::process] read 686522 sequences (100000175 bp) in 2.180 CPU sec, 2.183 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 280267, 9, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 258, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (282.85, 169.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 295, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (318.17, 159.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 273, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000) [M::mem_pestat] mean and std.dev: (279.62, 103.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1260) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687612 reads in 196.748 CPU sec, 6.511 real sec [M::mem_process_seqs] Processed 688134 reads in 129.273 CPU sec, 4.100 real sec sorting block 80, #reads = 1073870 mem = 536879817 peak = 1095970816 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 280221, 10, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 299, 544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1204) [M::mem_pestat] mean and std.dev: (375.11, 237.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 298, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (320.68, 159.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (256, 827, 3829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10975) [M::mem_pestat] mean and std.dev: (1766.70, 1647.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14548) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 402, 777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1913) [M::mem_pestat] mean and std.dev: (502.81, 363.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2481) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686368 sequences (100000242 bp) in 3.195 CPU sec, 3.449 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 279823, 8, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 308, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (323.50, 188.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1198) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 303, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (323.54, 159.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1138) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 341, 1239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3303) [M::mem_pestat] mean and std.dev: (593.97, 629.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4335) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687374 reads in 189.445 CPU sec, 6.013 real sec [M::process] read 686730 sequences (100000022 bp) in 2.255 CPU sec, 2.265 real sec... sorting block 81, #reads = 1072479 mem = 536879223 peak = 1095970816 [M::mem_process_seqs] Processed 686522 reads in 130.890 CPU sec, 4.139 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 279498, 5, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 303, 622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1478) [M::mem_pestat] mean and std.dev: (402.24, 329.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1906) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 303, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (324.82, 160.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1143) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 275, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (292.56, 84.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 82, #reads = 1073215 mem = 536877751 peak = 1095970816 [M::process] read 686392 sequences (100000088 bp) in 3.362 CPU sec, 3.591 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 279883, 14, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 306, 1229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3299) [M::mem_pestat] mean and std.dev: (380.27, 345.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4334) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 301, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (323.55, 160.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (417, 987, 3046) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8304) [M::mem_pestat] mean and std.dev: (1555.43, 1433.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10933) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 278, 554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1312) [M::mem_pestat] mean and std.dev: (330.19, 213.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1691) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686368 reads in 189.869 CPU sec, 6.018 real sec [M::process] read 686314 sequences (100000167 bp) in 2.313 CPU sec, 2.326 real sec... [M::mem_process_seqs] Processed 686730 reads in 130.730 CPU sec, 4.129 real sec sorting block 83, #reads = 1071664 mem = 536886131 peak = 1095970816 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 279480, 10, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 259, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1114) [M::mem_pestat] mean and std.dev: (252.32, 145.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1444) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 303, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (324.19, 159.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (205, 409, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (282.44, 154.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1169) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 308, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1058) [M::mem_pestat] mean and std.dev: (338.04, 192.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1339) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686354 sequences (100000225 bp) in 2.699 CPU sec, 2.797 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 279491, 7, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 297, 1209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3171) [M::mem_pestat] mean and std.dev: (570.38, 501.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4152) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 302, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (322.96, 158.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 289, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023) [M::mem_pestat] mean and std.dev: (310.91, 198.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1297) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686548 sequences (100000144 bp) in 1.700 CPU sec, 1.699 real sec... [M::mem_process_seqs] Processed 686392 reads in 186.055 CPU sec, 5.929 real sec [M::mem_process_seqs] Processed 686314 reads in 130.311 CPU sec, 4.146 real sec sorting block 84, #reads = 1071367 mem = 536880674 peak = 1095970816 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 279819, 10, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (254, 405, 644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1424) [M::mem_pestat] mean and std.dev: (391.38, 244.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1814) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 300, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (321.66, 157.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (152, 414, 2270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6506) [M::mem_pestat] mean and std.dev: (906.20, 1073.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8624) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 355, 864) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2212) [M::mem_pestat] mean and std.dev: (428.03, 362.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2886) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686358 sequences (100000147 bp) in 3.445 CPU sec, 3.718 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 279586, 14, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 239, 746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1968) [M::mem_pestat] mean and std.dev: (498.06, 613.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2950) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 300, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (321.01, 158.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (538, 939, 1173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2443) [M::mem_pestat] mean and std.dev: (760.08, 453.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3078) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 374, 565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1341) [M::mem_pestat] mean and std.dev: (331.16, 190.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1729) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686354 reads in 192.046 CPU sec, 6.096 real sec [M::process] read 686046 sequences (100000270 bp) in 1.848 CPU sec, 1.844 real sec... sorting block 85, #reads = 1072163 mem = 536879942 peak = 1095970816 [M::mem_process_seqs] Processed 686548 reads in 133.795 CPU sec, 4.240 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 279787, 7, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 358, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030) [M::mem_pestat] mean and std.dev: (343.86, 188.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1296) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 301, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (322.40, 159.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 254, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (318.00, 184.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 86, #reads = 1071229 mem = 536880869 peak = 1095970816 [M::process] read 686294 sequences (100000080 bp) in 2.821 CPU sec, 2.966 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 279208, 10, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 207, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (248.21, 142.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 301, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (322.75, 158.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (858, 1008, 2845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6819) [M::mem_pestat] mean and std.dev: (1232.00, 849.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8806) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 280, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (230.87, 101.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686254 sequences (100000196 bp) in 1.780 CPU sec, 1.787 real sec... [M::mem_process_seqs] Processed 686358 reads in 187.471 CPU sec, 5.962 real sec [M::mem_process_seqs] Processed 686046 reads in 129.581 CPU sec, 4.117 real sec sorting block 87, #reads = 1071617 mem = 536881652 peak = 1095970816 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 279859, 3, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 346, 551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1331) [M::mem_pestat] mean and std.dev: (365.45, 260.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 299, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (321.59, 158.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 194, 735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1879) [M::mem_pestat] mean and std.dev: (330.75, 254.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2451) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686042 sequences (100000200 bp) in 3.281 CPU sec, 3.397 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 279427, 12, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 259, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (282.36, 152.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1081) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 299, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (321.03, 157.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (309, 983, 1798) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4776) [M::mem_pestat] mean and std.dev: (939.58, 631.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6265) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 217, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (244.29, 111.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686026 sequences (100000242 bp) in 1.890 CPU sec, 1.888 real sec... [M::mem_process_seqs] Processed 686294 reads in 183.505 CPU sec, 5.811 real sec [M::mem_process_seqs] Processed 686254 reads in 126.824 CPU sec, 4.022 real sec sorting block 88, #reads = 1071988 mem = 536879872 peak = 1095970816 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 279304, 10, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 571, 1294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3530) [M::mem_pestat] mean and std.dev: (590.72, 499.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 300, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (321.95, 157.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1123) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (317, 2149, 2949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8213) [M::mem_pestat] mean and std.dev: (1865.90, 1702.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10845) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 338, 1085) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2889) [M::mem_pestat] mean and std.dev: (640.00, 637.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3791) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686242 sequences (100000135 bp) in 3.248 CPU sec, 3.484 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 280169, 21, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 277, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045) [M::mem_pestat] mean and std.dev: (281.48, 197.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1328) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 300, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (322.00, 158.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (287, 480, 1669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4433) [M::mem_pestat] mean and std.dev: (690.11, 603.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5815) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 300, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048) [M::mem_pestat] mean and std.dev: (331.71, 178.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1328) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685410 sequences (100000232 bp) in 1.819 CPU sec, 1.821 real sec... [M::mem_process_seqs] Processed 686042 reads in 182.495 CPU sec, 5.779 real sec [M::mem_process_seqs] Processed 686026 reads in 125.732 CPU sec, 3.987 real sec sorting block 89, #reads = 1071824 mem = 536878665 peak = 1095970816 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 279004, 8, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 307, 1393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3733) [M::mem_pestat] mean and std.dev: (602.98, 630.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4903) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 301, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (321.61, 157.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (293, 379, 602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1220) [M::mem_pestat] mean and std.dev: (401.00, 188.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1529) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685474 sequences (100000241 bp) in 3.098 CPU sec, 3.214 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 278927, 9, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 343, 506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1072) [M::mem_pestat] mean and std.dev: (348.30, 182.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 304, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (324.34, 157.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 293, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (295.50, 146.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685472 sequences (100000181 bp) in 2.210 CPU sec, 2.209 real sec... [M::mem_process_seqs] Processed 686242 reads in 189.866 CPU sec, 6.018 real sec sorting block 90, #reads = 1071461 mem = 536882116 peak = 1095970816 [M::mem_process_seqs] Processed 685410 reads in 133.991 CPU sec, 4.237 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 279828, 20, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 261, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (207.00, 79.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 303, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (324.19, 157.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (422, 1654, 1669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4163) [M::mem_pestat] mean and std.dev: (1177.11, 636.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5410) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (259, 416, 1503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3991) [M::mem_pestat] mean and std.dev: (749.43, 706.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5235) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 91, #reads = 1070835 mem = 536879451 peak = 1095970816 [M::process] read 685144 sequences (100000240 bp) in 3.526 CPU sec, 3.772 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 279199, 9, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 528, 673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1611) [M::mem_pestat] mean and std.dev: (488.75, 356.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2080) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 304, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (324.14, 158.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 377, 1508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4170) [M::mem_pestat] mean and std.dev: (494.90, 450.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5501) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685474 reads in 189.031 CPU sec, 5.984 real sec [M::process] read 685378 sequences (100000010 bp) in 2.374 CPU sec, 2.397 real sec... [M::mem_process_seqs] Processed 685472 reads in 131.527 CPU sec, 4.149 real sec sorting block 92, #reads = 1069837 mem = 536884084 peak = 1095970816 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 279644, 9, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 585, 891) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2247) [M::mem_pestat] mean and std.dev: (535.63, 353.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2925) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 302, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (322.26, 155.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 331, 1253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3389) [M::mem_pestat] mean and std.dev: (581.58, 515.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4457) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684784 sequences (100000164 bp) in 3.416 CPU sec, 3.631 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 279761, 10, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (331, 388, 1168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2842) [M::mem_pestat] mean and std.dev: (585.17, 467.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 304, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (323.19, 156.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (496, 1170, 1170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2518) [M::mem_pestat] mean and std.dev: (865.00, 490.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3192) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 413, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1144) [M::mem_pestat] mean and std.dev: (394.57, 219.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1450) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685144 reads in 185.184 CPU sec, 5.883 real sec [M::mem_process_seqs] Processed 685378 reads in 129.159 CPU sec, 4.069 real sec [M::process] read 684546 sequences (100000010 bp) in 2.490 CPU sec, 2.547 real sec... sorting block 93, #reads = 1072148 mem = 536877788 peak = 1095970816 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 279520, 15, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (358, 391, 1042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2410) [M::mem_pestat] mean and std.dev: (646.67, 623.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3140) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 306, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (325.51, 157.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (463, 1324, 1711) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4207) [M::mem_pestat] mean and std.dev: (968.15, 626.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5455) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 303, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962) [M::mem_pestat] mean and std.dev: (312.20, 123.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1207) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684266 sequences (100000296 bp) in 3.259 CPU sec, 3.449 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 279514, 11, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 335, 701) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1669) [M::mem_pestat] mean and std.dev: (355.23, 194.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2153) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 309, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (327.85, 158.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (710, 986, 1141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2003) [M::mem_pestat] mean and std.dev: (815.80, 454.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2633) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 294, 602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1414) [M::mem_pestat] mean and std.dev: (366.84, 235.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1820) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684784 reads in 182.341 CPU sec, 5.793 real sec [M::process] read 684264 sequences (100000216 bp) in 1.815 CPU sec, 1.828 real sec... sorting block 94, #reads = 1070845 mem = 536878132 peak = 1095970816 [M::mem_process_seqs] Processed 684546 reads in 127.450 CPU sec, 4.033 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 279348, 18, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 240, 760) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1816) [M::mem_pestat] mean and std.dev: (466.07, 312.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2344) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 312, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (330.70, 160.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1142) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 541, 856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2528) [M::mem_pestat] mean and std.dev: (563.11, 568.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3364) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 286, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (266.46, 88.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 95, #reads = 1070656 mem = 536878786 peak = 1095970816 [M::process] read 689620 sequences (100000284 bp) in 3.066 CPU sec, 3.349 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 278974, 17, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 454, 1306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3536) [M::mem_pestat] mean and std.dev: (716.65, 688.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 313, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (332.44, 162.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (56, 1030, 4745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14123) [M::mem_pestat] mean and std.dev: (2025.53, 2099.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18812) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 222, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1112) [M::mem_pestat] mean and std.dev: (316.07, 256.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1430) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694670 sequences (100000045 bp) in 1.780 CPU sec, 1.780 real sec... [M::mem_process_seqs] Processed 684266 reads in 184.224 CPU sec, 5.844 real sec [M::mem_process_seqs] Processed 684264 reads in 129.117 CPU sec, 4.094 real sec sorting block 96, #reads = 1069964 mem = 536878488 peak = 1095970816 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 281059, 12, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 270, 582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1340) [M::mem_pestat] mean and std.dev: (363.12, 245.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1719) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 287, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (312.60, 158.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (758, 906, 983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (308, 1433) [M::mem_pestat] mean and std.dev: (908.29, 69.51) [M::mem_pestat] low and high boundaries for proper pairs: (83, 1658) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (121, 354, 651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1711) [M::mem_pestat] mean and std.dev: (321.62, 242.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2241) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693336 sequences (100000165 bp) in 3.135 CPU sec, 3.287 real sec... [M::process] read 692210 sequences (100000112 bp) in 1.780 CPU sec, 1.784 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282839, 18, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 313, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1162) [M::mem_pestat] mean and std.dev: (373.72, 261.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 264, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (295.15, 154.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (407, 786, 1226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2864) [M::mem_pestat] mean and std.dev: (680.20, 381.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3683) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 227, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (245.91, 99.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689620 reads in 200.688 CPU sec, 6.371 real sec [M::mem_process_seqs] Processed 694670 reads in 139.652 CPU sec, 4.440 real sec sorting block 97, #reads = 1070155 mem = 536878843 peak = 1095970816 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282952, 8, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 258, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021) [M::mem_pestat] mean and std.dev: (262.78, 124.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1304) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 270, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (300.42, 156.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1079) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (261, 313, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041) [M::mem_pestat] mean and std.dev: (322.71, 144.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1301) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 691592 sequences (100000285 bp) in 3.582 CPU sec, 3.714 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 282564, 24, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 235, 565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1331) [M::mem_pestat] mean and std.dev: (340.56, 253.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 275, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (303.83, 157.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (193, 420, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1138) [M::mem_pestat] mean and std.dev: (315.95, 178.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1453) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 361, 603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1459) [M::mem_pestat] mean and std.dev: (427.10, 332.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1887) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693336 reads in 186.036 CPU sec, 5.883 real sec [M::mem_process_seqs] Processed 692210 reads in 125.500 CPU sec, 3.951 real sec [M::process] read 691318 sequences (100000209 bp) in 2.896 CPU sec, 2.958 real sec... sorting block 98, #reads = 1075424 mem = 536880890 peak = 1095970816 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 282309, 8, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 178, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (181.94, 90.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 278, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (306.40, 158.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (247, 438, 1459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3883) [M::mem_pestat] mean and std.dev: (920.59, 1032.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5095) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 690688 sequences (100000083 bp) in 3.399 CPU sec, 3.554 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 282223, 10, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 234, 599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1497) [M::mem_pestat] mean and std.dev: (405.50, 359.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1946) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 279, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (306.58, 157.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (118, 492, 1864) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5356) [M::mem_pestat] mean and std.dev: (1019.90, 941.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7102) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 260, 1115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2965) [M::mem_pestat] mean and std.dev: (659.81, 827.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3971) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691592 reads in 180.962 CPU sec, 5.746 real sec sorting block 99, #reads = 1073312 mem = 536883155 peak = 1095970816 [M::mem_process_seqs] Processed 691318 reads in 126.609 CPU sec, 3.986 real sec [M::process] read 690624 sequences (100000034 bp) in 2.795 CPU sec, 2.929 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 281769, 15, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 314, 811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2053) [M::mem_pestat] mean and std.dev: (436.83, 397.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 281, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (307.96, 157.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (758, 919, 1379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2621) [M::mem_pestat] mean and std.dev: (1048.40, 500.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3242) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 246, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (248.26, 114.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 995) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 100, #reads = 1073920 mem = 536878223 peak = 1095970816 [M::process] read 690284 sequences (100000056 bp) in 3.367 CPU sec, 3.528 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 282512, 22, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 242, 1747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4891) [M::mem_pestat] mean and std.dev: (848.77, 983.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 285, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (311.57, 159.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (292, 871, 1663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4405) [M::mem_pestat] mean and std.dev: (912.36, 748.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5776) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (252, 381, 605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1311) [M::mem_pestat] mean and std.dev: (417.86, 246.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1664) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690688 reads in 189.247 CPU sec, 5.999 real sec [M::mem_process_seqs] Processed 690624 reads in 131.523 CPU sec, 4.141 real sec [M::process] read 689504 sequences (100000151 bp) in 2.593 CPU sec, 2.658 real sec... sorting block 101, #reads = 1073118 mem = 536881213 peak = 1095970816 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 281562, 9, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 364, 675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1655) [M::mem_pestat] mean and std.dev: (360.63, 223.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2145) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 285, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (311.23, 159.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (305, 512, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1067) [M::mem_pestat] mean and std.dev: (408.00, 161.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1321) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689112 sequences (100000156 bp) in 3.479 CPU sec, 3.635 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 281928, 7, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (250, 438, 1604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4312) [M::mem_pestat] mean and std.dev: (832.82, 821.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 290, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (315.83, 161.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 336, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844) [M::mem_pestat] mean and std.dev: (300.85, 116.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690284 reads in 203.514 CPU sec, 6.446 real sec [M::process] read 688696 sequences (100000277 bp) in 2.618 CPU sec, 2.659 real sec... [M::mem_process_seqs] Processed 689504 reads in 133.374 CPU sec, 4.195 real sec sorting block 102, #reads = 1073097 mem = 536879990 peak = 1095970816 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 281178, 5, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 274, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (242.23, 94.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 292, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (317.55, 162.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1142) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 541, 542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1194) [M::mem_pestat] mean and std.dev: (480.29, 270.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1561) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688584 sequences (100000067 bp) in 3.508 CPU sec, 3.649 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 281272, 15, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 418, 2313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6641) [M::mem_pestat] mean and std.dev: (967.95, 1254.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8805) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 294, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (319.27, 161.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (81, 543, 678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1872) [M::mem_pestat] mean and std.dev: (481.67, 442.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2469) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 314, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041) [M::mem_pestat] mean and std.dev: (342.38, 205.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1317) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689112 reads in 184.821 CPU sec, 5.854 real sec [M::mem_process_seqs] Processed 688696 reads in 127.111 CPU sec, 4.003 real sec sorting block 103, #reads = 1070842 mem = 536880324 peak = 1095970816 [M::process] read 688260 sequences (100000009 bp) in 2.996 CPU sec, 3.093 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 280847, 19, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 288, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1189) [M::mem_pestat] mean and std.dev: (286.48, 128.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 294, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (319.45, 162.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (870, 1270, 1270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (70, 2070) [M::mem_pestat] mean and std.dev: (1102.65, 258.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2470) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 349, 760) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1802) [M::mem_pestat] mean and std.dev: (433.22, 267.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2323) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 104, #reads = 1073594 mem = 536880259 peak = 1095970816 [M::process] read 688084 sequences (100000250 bp) in 3.431 CPU sec, 3.649 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 280999, 13, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 362, 807) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2001) [M::mem_pestat] mean and std.dev: (507.16, 422.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 298, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (322.32, 162.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1158) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 672, 1570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4646) [M::mem_pestat] mean and std.dev: (966.15, 1130.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6184) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 439, 1235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3251) [M::mem_pestat] mean and std.dev: (713.47, 596.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4259) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688584 reads in 200.809 CPU sec, 6.383 real sec [M::mem_process_seqs] Processed 688260 reads in 146.564 CPU sec, 4.627 real sec [M::process] read 687618 sequences (100000167 bp) in 2.823 CPU sec, 2.912 real sec... sorting block 105, #reads = 1071107 mem = 536877240 peak = 1095970816 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 280555, 12, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 242, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (234.50, 134.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 297, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (321.26, 162.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (164, 634, 3113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9011) [M::mem_pestat] mean and std.dev: (1529.75, 1676.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11960) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 234, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (306.59, 178.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1026) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687688 sequences (100000004 bp) in 3.098 CPU sec, 3.271 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 280790, 9, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 348, 615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1417) [M::mem_pestat] mean and std.dev: (342.41, 202.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1818) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 299, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (323.37, 163.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1166) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 419, 580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1254) [M::mem_pestat] mean and std.dev: (362.25, 202.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1591) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686922 sequences (100000181 bp) in 1.889 CPU sec, 1.888 real sec... [M::mem_process_seqs] Processed 688084 reads in 214.393 CPU sec, 6.829 real sec sorting block 106, #reads = 1070320 mem = 536882942 peak = 1095970816 [M::mem_process_seqs] Processed 687618 reads in 154.300 CPU sec, 4.901 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 280426, 21, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 225, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1191) [M::mem_pestat] mean and std.dev: (263.79, 181.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 299, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (323.35, 162.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1159) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (421, 789, 934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1960) [M::mem_pestat] mean and std.dev: (544.53, 300.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2473) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 237, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (265.52, 110.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686986 sequences (100000286 bp) in 3.622 CPU sec, 3.874 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 280278, 8, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 376, 850) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2112) [M::mem_pestat] mean and std.dev: (456.12, 352.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 301, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (325.34, 163.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1165) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 281, 560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1258) [M::mem_pestat] mean and std.dev: (288.86, 148.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1607) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687160 sequences (100000145 bp) in 2.440 CPU sec, 2.438 real sec... [M::mem_process_seqs] Processed 687688 reads in 217.699 CPU sec, 6.885 real sec sorting block 107, #reads = 1070574 mem = 536884265 peak = 1095970816 [M::mem_process_seqs] Processed 686922 reads in 260.986 CPU sec, 8.287 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 280297, 12, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 326, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (310.45, 151.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1100) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 298, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (322.45, 161.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (458, 594, 1658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4058) [M::mem_pestat] mean and std.dev: (890.25, 592.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5258) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 327, 1055) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2697) [M::mem_pestat] mean and std.dev: (740.12, 784.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3878) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686716 sequences (100000001 bp) in 3.759 CPU sec, 3.983 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 279643, 23, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 250, 740) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1868) [M::mem_pestat] mean and std.dev: (393.56, 384.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 300, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (324.00, 161.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (341, 1028, 1617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4169) [M::mem_pestat] mean and std.dev: (872.48, 655.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5445) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 295, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (240.56, 94.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686746 sequences (100000281 bp) in 2.423 CPU sec, 2.428 real sec... [M::mem_process_seqs] Processed 687160 reads in 217.060 CPU sec, 6.856 real sec sorting block 108, #reads = 1069038 mem = 536880436 peak = 1095970816 [M::mem_process_seqs] Processed 686986 reads in 248.559 CPU sec, 7.881 real sec sorting block 109, #reads = 1069125 mem = 536880356 peak = 1104076800 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 279752, 9, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 343, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1111) [M::mem_pestat] mean and std.dev: (313.50, 195.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 299, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (323.24, 161.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 317, 822) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1998) [M::mem_pestat] mean and std.dev: (468.37, 314.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2586) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686928 sequences (100000090 bp) in 3.374 CPU sec, 3.589 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 280410, 16, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 273, 1023) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2683) [M::mem_pestat] mean and std.dev: (485.86, 501.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 300, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (323.77, 161.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (155, 564, 1637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4601) [M::mem_pestat] mean and std.dev: (733.06, 681.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6083) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 270, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1112) [M::mem_pestat] mean and std.dev: (295.41, 122.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1413) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687028 sequences (100000144 bp) in 2.360 CPU sec, 2.359 real sec... [M::mem_process_seqs] Processed 686716 reads in 213.619 CPU sec, 6.769 real sec [M::mem_process_seqs] Processed 686746 reads in 148.409 CPU sec, 4.705 real sec sorting block 110, #reads = 1068971 mem = 536877541 peak = 1104076800 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 280230, 15, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 316, 675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1663) [M::mem_pestat] mean and std.dev: (353.24, 256.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2157) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 299, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (322.89, 161.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (493, 575, 6031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17107) [M::mem_pestat] mean and std.dev: (2064.53, 2500.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22645) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 230, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973) [M::mem_pestat] mean and std.dev: (275.37, 124.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1236) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686826 sequences (100000108 bp) in 3.847 CPU sec, 4.102 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 280401, 17, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (272, 374, 1105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2771) [M::mem_pestat] mean and std.dev: (639.72, 632.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 298, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (321.68, 160.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (318, 4217, 5878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16998) [M::mem_pestat] mean and std.dev: (3214.29, 2668.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22558) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 325, 546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1222) [M::mem_pestat] mean and std.dev: (307.05, 150.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1560) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686742 sequences (100000215 bp) in 2.145 CPU sec, 2.153 real sec... [M::mem_process_seqs] Processed 686928 reads in 212.324 CPU sec, 6.719 real sec sorting block 111, #reads = 1069268 mem = 536882083 peak = 1104076800 [M::mem_process_seqs] Processed 687028 reads in 149.978 CPU sec, 4.750 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 280224, 13, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 235, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993) [M::mem_pestat] mean and std.dev: (273.76, 175.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1263) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 299, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (322.34, 160.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (321, 490, 1987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5319) [M::mem_pestat] mean and std.dev: (528.00, 504.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6985) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 251, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1224) [M::mem_pestat] mean and std.dev: (347.45, 237.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1567) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685894 sequences (100000186 bp) in 3.596 CPU sec, 3.850 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 280565, 10, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 300, 555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1301) [M::mem_pestat] mean and std.dev: (323.34, 178.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 298, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (321.82, 159.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (631, 916, 1193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2317) [M::mem_pestat] mean and std.dev: (862.10, 329.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2879) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 344, 1596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4332) [M::mem_pestat] mean and std.dev: (817.21, 984.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5700) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686826 reads in 217.490 CPU sec, 6.885 real sec [M::process] read 686680 sequences (100000290 bp) in 2.579 CPU sec, 2.574 real sec... sorting block 112, #reads = 1070523 mem = 536881226 peak = 1104076800 [M::mem_process_seqs] Processed 686742 reads in 154.872 CPU sec, 4.893 real sec sorting block 113, #reads = 1068478 mem = 536878105 peak = 1104076800 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 279894, 15, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (238, 355, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (327.46, 127.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 305, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (326.99, 161.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (823, 1223, 3054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7516) [M::mem_pestat] mean and std.dev: (1690.73, 1343.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9747) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 288, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (277.48, 139.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686458 sequences (100000029 bp) in 3.411 CPU sec, 3.546 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 279491, 17, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 272, 701) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1759) [M::mem_pestat] mean and std.dev: (343.97, 250.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2288) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 299, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (321.15, 158.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (474, 687, 1211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2685) [M::mem_pestat] mean and std.dev: (845.65, 494.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3422) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 289, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (327.86, 167.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686422 sequences (100000145 bp) in 2.740 CPU sec, 2.732 real sec... [M::mem_process_seqs] Processed 685894 reads in 219.654 CPU sec, 6.947 real sec [M::mem_process_seqs] Processed 686680 reads in 152.028 CPU sec, 4.809 real sec sorting block 114, #reads = 1067045 mem = 536880505 peak = 1104076800 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 279983, 6, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 331, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (320.21, 148.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1016) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 299, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (321.10, 158.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 401, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1065) [M::mem_pestat] mean and std.dev: (380.59, 179.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1342) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686380 sequences (100000052 bp) in 4.014 CPU sec, 4.164 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 279769, 9, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 273, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (277.27, 134.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 300, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (320.82, 157.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 254, 589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1397) [M::mem_pestat] mean and std.dev: (328.64, 191.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1801) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686314 sequences (100000257 bp) in 2.380 CPU sec, 2.383 real sec... [M::mem_process_seqs] Processed 686458 reads in 212.919 CPU sec, 6.734 real sec sorting block 115, #reads = 1068841 mem = 536879240 peak = 1104076800 [M::mem_process_seqs] Processed 686422 reads in 151.465 CPU sec, 4.787 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 279886, 12, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 399, 596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1484) [M::mem_pestat] mean and std.dev: (400.27, 318.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1928) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 300, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (320.57, 156.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (255, 780, 1429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3777) [M::mem_pestat] mean and std.dev: (963.92, 820.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4951) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 261, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (264.19, 101.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686306 sequences (100000269 bp) in 3.584 CPU sec, 3.749 real sec... [M::process] read 685962 sequences (100000134 bp) in 2.012 CPU sec, 2.020 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 279319, 7, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 234, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021) [M::mem_pestat] mean and std.dev: (282.73, 167.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1304) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 300, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (321.27, 157.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (262, 428, 959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2353) [M::mem_pestat] mean and std.dev: (509.04, 314.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3050) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686380 reads in 216.326 CPU sec, 6.863 real sec sorting block 116, #reads = 1067581 mem = 536882167 peak = 1104076800 [M::mem_process_seqs] Processed 686314 reads in 151.282 CPU sec, 4.811 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 279720, 4, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 319, 1060) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2788) [M::mem_pestat] mean and std.dev: (669.29, 668.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 300, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (322.01, 158.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 241, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1148) [M::mem_pestat] mean and std.dev: (277.54, 188.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1477) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 117, #reads = 1067211 mem = 536879459 peak = 1104076800 [M::process] read 686180 sequences (100000025 bp) in 3.406 CPU sec, 3.647 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 280353, 11, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 377, 976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2500) [M::mem_pestat] mean and std.dev: (690.12, 697.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 302, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (323.91, 159.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (664, 862, 1729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3859) [M::mem_pestat] mean and std.dev: (1139.91, 743.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4924) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 312, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1196) [M::mem_pestat] mean and std.dev: (370.50, 233.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1529) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686210 sequences (100000295 bp) in 2.390 CPU sec, 2.393 real sec... [M::mem_process_seqs] Processed 686306 reads in 222.873 CPU sec, 7.362 real sec [M::mem_process_seqs] Processed 685962 reads in 148.382 CPU sec, 4.703 real sec sorting block 118, #reads = 1068252 mem = 536880696 peak = 1104076800 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 280228, 16, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 254, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1061) [M::mem_pestat] mean and std.dev: (267.21, 131.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1343) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 300, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (323.42, 160.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (386, 990, 1945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5063) [M::mem_pestat] mean and std.dev: (719.46, 577.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6622) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 341, 606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1438) [M::mem_pestat] mean and std.dev: (387.39, 247.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1854) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695186 sequences (100000205 bp) in 3.604 CPU sec, 3.827 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 279417, 13, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 317, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038) [M::mem_pestat] mean and std.dev: (312.92, 173.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1314) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 301, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (326.13, 164.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (390, 441, 1525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3795) [M::mem_pestat] mean and std.dev: (831.08, 781.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4930) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 270, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (272.03, 102.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695822 sequences (100000183 bp) in 2.400 CPU sec, 2.404 real sec... [M::mem_process_seqs] Processed 686180 reads in 218.817 CPU sec, 6.933 real sec [M::mem_process_seqs] Processed 686210 reads in 153.595 CPU sec, 4.860 real sec sorting block 119, #reads = 1066708 mem = 536880581 peak = 1104076800 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283350, 9, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 353, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1237) [M::mem_pestat] mean and std.dev: (301.00, 139.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 261, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (291.17, 151.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 275, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (289.52, 108.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1014) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693958 sequences (100000103 bp) in 4.234 CPU sec, 4.464 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283434, 13, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 182, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052) [M::mem_pestat] mean and std.dev: (268.78, 181.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1351) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 259, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (288.29, 149.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (120, 495, 2146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6198) [M::mem_pestat] mean and std.dev: (837.67, 841.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8224) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 244, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (283.85, 129.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693456 sequences (100000078 bp) in 2.448 CPU sec, 2.455 real sec... [M::mem_process_seqs] Processed 695186 reads in 226.875 CPU sec, 7.179 real sec sorting block 120, #reads = 1066571 mem = 536878933 peak = 1104076800 [M::mem_process_seqs] Processed 695822 reads in 157.519 CPU sec, 4.988 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 283046, 20, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 341, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942) [M::mem_pestat] mean and std.dev: (310.29, 122.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1185) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 266, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (293.48, 150.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (252, 406, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1092) [M::mem_pestat] mean and std.dev: (376.89, 211.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1372) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 357, 851) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2181) [M::mem_pestat] mean and std.dev: (468.12, 465.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2846) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692502 sequences (100000240 bp) in 3.333 CPU sec, 3.560 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282425, 6, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 330, 856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2288) [M::mem_pestat] mean and std.dev: (388.13, 328.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3004) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 269, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (296.22, 151.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 427, 888) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2262) [M::mem_pestat] mean and std.dev: (511.76, 448.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2949) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692064 sequences (100000103 bp) in 2.270 CPU sec, 2.272 real sec... [M::mem_process_seqs] Processed 693958 reads in 216.890 CPU sec, 6.886 real sec sorting block 121, #reads = 1070270 mem = 536877929 peak = 1104076800 [M::mem_process_seqs] Processed 693456 reads in 153.305 CPU sec, 4.857 real sec sorting block 122, #reads = 1070039 mem = 536878656 peak = 1109643264 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 282701, 8, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 268, 1861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5213) [M::mem_pestat] mean and std.dev: (574.24, 639.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6889) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 274, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849) [M::mem_pestat] mean and std.dev: (300.31, 152.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1073) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 233, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (219.20, 97.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692280 sequences (100000030 bp) in 3.075 CPU sec, 3.237 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282730, 15, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 256, 4491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13065) [M::mem_pestat] mean and std.dev: (1547.08, 2009.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17352) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 277, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (303.62, 155.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1090) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (395, 708, 3628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10094) [M::mem_pestat] mean and std.dev: (1925.13, 2124.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13327) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (135, 260, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (204.17, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692066 sequences (100000142 bp) in 2.480 CPU sec, 2.480 real sec... [M::mem_process_seqs] Processed 692502 reads in 213.571 CPU sec, 6.762 real sec [M::mem_process_seqs] Processed 692064 reads in 152.174 CPU sec, 4.820 real sec sorting block 123, #reads = 1070701 mem = 536883783 peak = 1109643264 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282752, 14, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 241, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955) [M::mem_pestat] mean and std.dev: (247.00, 151.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1226) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 274, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (301.78, 154.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1078) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (138, 854, 1162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3210) [M::mem_pestat] mean and std.dev: (887.15, 848.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4279) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 395, 2180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6114) [M::mem_pestat] mean and std.dev: (1056.84, 987.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8081) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692282 sequences (100000260 bp) in 3.145 CPU sec, 3.317 real sec... [M::process] read 691368 sequences (100000116 bp) in 2.294 CPU sec, 2.297 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 282344, 12, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 245, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (242.85, 134.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 274, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (300.73, 152.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1074) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (693, 2226, 2226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5292) [M::mem_pestat] mean and std.dev: (1750.50, 1364.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7209) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 319, 863) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2203) [M::mem_pestat] mean and std.dev: (449.27, 415.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2873) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692280 reads in 219.788 CPU sec, 6.968 real sec sorting block 124, #reads = 1068844 mem = 536882257 peak = 1109643264 [M::mem_process_seqs] Processed 692066 reads in 154.923 CPU sec, 4.922 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282798, 14, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 337, 1360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3554) [M::mem_pestat] mean and std.dev: (663.00, 750.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 274, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (301.16, 153.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1081) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 650, 1080) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3182) [M::mem_pestat] mean and std.dev: (557.29, 476.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4233) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 345, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1184) [M::mem_pestat] mean and std.dev: (340.93, 192.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1515) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 690618 sequences (100000043 bp) in 3.468 CPU sec, 3.590 real sec... [M::process] read 690186 sequences (100000134 bp) in 1.930 CPU sec, 1.934 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282197, 6, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 322, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004) [M::mem_pestat] mean and std.dev: (280.90, 169.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1292) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 278, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (305.77, 156.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 436, 981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2501) [M::mem_pestat] mean and std.dev: (643.73, 521.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3261) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692282 reads in 219.803 CPU sec, 6.971 real sec sorting block 125, #reads = 1068627 mem = 536877185 peak = 1109643264 [M::mem_process_seqs] Processed 691368 reads in 163.535 CPU sec, 5.222 real sec sorting block 126, #reads = 1067769 mem = 536880533 peak = 1109643264 [M::process] read 689918 sequences (100000118 bp) in 3.317 CPU sec, 3.507 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 281896, 12, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 283, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028) [M::mem_pestat] mean and std.dev: (295.56, 174.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1307) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 286, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (311.00, 157.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (273, 408, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (315.82, 160.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (284, 723, 1271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3245) [M::mem_pestat] mean and std.dev: (805.96, 632.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4232) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689542 sequences (100000265 bp) in 1.870 CPU sec, 1.879 real sec... [M::mem_process_seqs] Processed 690186 reads in 118.499 CPU sec, 3.753 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 282156, 9, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (229, 288, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093) [M::mem_pestat] mean and std.dev: (284.34, 141.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1381) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 283, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (308.63, 156.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 321, 580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1278) [M::mem_pestat] mean and std.dev: (373.10, 214.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1627) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 282095, 14, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 233, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (226.02, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 287, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (311.86, 157.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (258, 1372, 3672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10500) [M::mem_pestat] mean and std.dev: (2511.86, 2760.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13914) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 290, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1122) [M::mem_pestat] mean and std.dev: (354.25, 224.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1425) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689574 sequences (100000083 bp) in 2.331 CPU sec, 2.333 real sec... [M::mem_process_seqs] Processed 690618 reads in 310.209 CPU sec, 9.842 real sec sorting block 127, #reads = 1070334 mem = 536876613 peak = 1109643264 [M::mem_process_seqs] Processed 689918 reads in 174.799 CPU sec, 5.579 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 282001, 13, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 226, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (226.71, 75.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 289, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (313.70, 158.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (104, 787, 1593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4571) [M::mem_pestat] mean and std.dev: (870.46, 775.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6060) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 237, 743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1879) [M::mem_pestat] mean and std.dev: (396.97, 327.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2447) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 128, #reads = 1070824 mem = 536883762 peak = 1109643264 [M::process] read 689076 sequences (100000244 bp) in 3.363 CPU sec, 3.578 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 281640, 7, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 325, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (285.78, 133.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 288, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (313.83, 159.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 383, 535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1223) [M::mem_pestat] mean and std.dev: (322.87, 156.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1567) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688148 sequences (100000299 bp) in 2.342 CPU sec, 2.349 real sec... [M::mem_process_seqs] Processed 689542 reads in 201.406 CPU sec, 6.372 real sec [M::mem_process_seqs] Processed 689574 reads in 141.377 CPU sec, 4.470 real sec sorting block 129, #reads = 1070468 mem = 536882036 peak = 1109643264 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 280765, 13, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 290, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954) [M::mem_pestat] mean and std.dev: (300.59, 158.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1209) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 292, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (316.29, 160.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (232, 479, 823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2005) [M::mem_pestat] mean and std.dev: (565.08, 429.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2596) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (260, 353, 1397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3671) [M::mem_pestat] mean and std.dev: (636.03, 828.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4808) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688336 sequences (100000111 bp) in 3.317 CPU sec, 3.589 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281300, 10, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 243, 1537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4275) [M::mem_pestat] mean and std.dev: (768.35, 893.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 295, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (319.47, 160.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (387, 585, 1882) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4872) [M::mem_pestat] mean and std.dev: (1178.80, 1358.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6613) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (318, 528, 618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1218) [M::mem_pestat] mean and std.dev: (477.49, 243.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1518) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687620 sequences (100000271 bp) in 2.240 CPU sec, 2.234 real sec... [M::mem_process_seqs] Processed 689076 reads in 217.461 CPU sec, 7.207 real sec [M::mem_process_seqs] Processed 688148 reads in 149.085 CPU sec, 4.730 real sec sorting block 130, #reads = 1068988 mem = 536877767 peak = 1109643264 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 281153, 8, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 309, 730) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1832) [M::mem_pestat] mean and std.dev: (424.03, 314.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2383) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 296, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (319.96, 160.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 388, 540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1206) [M::mem_pestat] mean and std.dev: (346.89, 200.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1539) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687648 sequences (100000195 bp) in 2.945 CPU sec, 3.068 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 281115, 14, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 249, 614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1502) [M::mem_pestat] mean and std.dev: (364.60, 290.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1946) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 297, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (321.55, 161.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (693, 1220, 1403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2823) [M::mem_pestat] mean and std.dev: (1057.83, 541.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3533) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 264, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (262.15, 148.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687654 sequences (100000101 bp) in 2.280 CPU sec, 2.287 real sec... [M::mem_process_seqs] Processed 688336 reads in 199.798 CPU sec, 6.328 real sec sorting block 131, #reads = 1067871 mem = 536885884 peak = 1109643264 [M::mem_process_seqs] Processed 687620 reads in 138.100 CPU sec, 4.377 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 280569, 14, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 249, 569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1349) [M::mem_pestat] mean and std.dev: (341.16, 243.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1739) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 295, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (320.07, 161.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1142) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (345, 839, 1432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3606) [M::mem_pestat] mean and std.dev: (847.38, 759.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4693) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 258, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006) [M::mem_pestat] mean and std.dev: (292.06, 167.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1277) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687656 sequences (100000247 bp) in 3.387 CPU sec, 3.632 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 279996, 8, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 310, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961) [M::mem_pestat] mean and std.dev: (319.33, 146.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1208) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 295, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (320.67, 161.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 239, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1131) [M::mem_pestat] mean and std.dev: (281.13, 148.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1446) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686966 sequences (100000019 bp) in 2.020 CPU sec, 2.021 real sec... [M::mem_process_seqs] Processed 687648 reads in 200.264 CPU sec, 6.339 real sec sorting block 132, #reads = 1068019 mem = 536886815 peak = 1109643264 [M::mem_process_seqs] Processed 687654 reads in 141.742 CPU sec, 4.494 real sec sorting block 133, #reads = 1067905 mem = 536879705 peak = 1114210304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 280714, 6, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 230, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (244.77, 117.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 296, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (321.83, 162.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 268, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1064) [M::mem_pestat] mean and std.dev: (321.94, 212.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1364) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686968 sequences (100000134 bp) in 3.268 CPU sec, 3.479 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 280397, 8, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (229, 285, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (247.29, 78.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 299, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (323.33, 162.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 300, 715) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1775) [M::mem_pestat] mean and std.dev: (426.23, 339.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2305) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686670 sequences (100000148 bp) in 2.280 CPU sec, 2.277 real sec... [M::mem_process_seqs] Processed 687656 reads in 198.876 CPU sec, 6.290 real sec [M::mem_process_seqs] Processed 686966 reads in 139.747 CPU sec, 4.417 real sec sorting block 134, #reads = 1068917 mem = 536879338 peak = 1114210304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 280162, 6, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 327, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (334.06, 214.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1191) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 298, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (322.72, 161.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 270, 561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1351) [M::mem_pestat] mean and std.dev: (301.15, 167.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1746) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686746 sequences (100000286 bp) in 3.529 CPU sec, 3.767 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 280006, 16, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 265, 956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2454) [M::mem_pestat] mean and std.dev: (622.78, 631.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3203) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 299, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (323.10, 161.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (146, 693, 864) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2300) [M::mem_pestat] mean and std.dev: (428.08, 335.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3018) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 259, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (254.36, 125.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686776 sequences (100000233 bp) in 2.300 CPU sec, 2.296 real sec... [M::mem_process_seqs] Processed 686968 reads in 195.242 CPU sec, 6.157 real sec sorting block 135, #reads = 1068815 mem = 536877968 peak = 1114210304 [M::mem_process_seqs] Processed 686670 reads in 221.505 CPU sec, 7.017 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 280143, 12, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 332, 863) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2205) [M::mem_pestat] mean and std.dev: (395.94, 307.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2876) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 301, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (325.07, 163.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (80, 201, 674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1862) [M::mem_pestat] mean and std.dev: (310.75, 345.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2456) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 332, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953) [M::mem_pestat] mean and std.dev: (340.02, 183.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1209) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686626 sequences (100000041 bp) in 3.222 CPU sec, 3.437 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 280274, 5, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 281, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (252.55, 80.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 301, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (326.70, 165.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1173) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 325, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1085) [M::mem_pestat] mean and std.dev: (314.94, 148.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1371) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686366 sequences (100000205 bp) in 2.250 CPU sec, 2.251 real sec... [M::mem_process_seqs] Processed 686746 reads in 194.756 CPU sec, 6.403 real sec sorting block 136, #reads = 1069902 mem = 536880023 peak = 1114210304 [M::mem_process_seqs] Processed 686776 reads in 134.113 CPU sec, 4.263 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 280360, 14, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (296, 1234, 1687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4469) [M::mem_pestat] mean and std.dev: (907.81, 748.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5860) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 302, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (327.14, 165.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1169) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (203, 761, 1778) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4928) [M::mem_pestat] mean and std.dev: (1075.50, 961.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6503) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 325, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (289.50, 99.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1086) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 137, #reads = 1068525 mem = 536879296 peak = 1114210304 [M::process] read 686366 sequences (100000001 bp) in 2.840 CPU sec, 3.068 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 280254, 11, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 300, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (284.89, 109.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 302, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (328.34, 166.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (432, 834, 1829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4623) [M::mem_pestat] mean and std.dev: (897.60, 707.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6020) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (262, 374, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036) [M::mem_pestat] mean and std.dev: (375.38, 170.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1294) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686626 reads in 169.963 CPU sec, 5.397 real sec [M::process] read 686000 sequences (100000297 bp) in 1.925 CPU sec, 1.940 real sec... [M::mem_process_seqs] Processed 686366 reads in 108.804 CPU sec, 3.433 real sec sorting block 138, #reads = 1069231 mem = 536882236 peak = 1114210304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 280283, 14, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 340, 627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1539) [M::mem_pestat] mean and std.dev: (405.76, 263.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1995) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 304, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (328.96, 166.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1171) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (839, 2961, 3064) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7514) [M::mem_pestat] mean and std.dev: (2093.64, 1389.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9739) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 360, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (343.95, 168.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686070 sequences (100000087 bp) in 2.917 CPU sec, 3.013 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 279115, 10, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 317, 598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1396) [M::mem_pestat] mean and std.dev: (406.83, 284.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1795) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 304, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936) [M::mem_pestat] mean and std.dev: (330.69, 168.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1183) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (452, 1799, 2508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6620) [M::mem_pestat] mean and std.dev: (1643.50, 981.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8676) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 299, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (291.65, 133.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686366 reads in 163.010 CPU sec, 5.408 real sec [M::process] read 686542 sequences (100000155 bp) in 2.440 CPU sec, 2.477 real sec... [M::mem_process_seqs] Processed 686000 reads in 123.752 CPU sec, 3.921 real sec sorting block 139, #reads = 1068440 mem = 536882090 peak = 1114210304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 280718, 14, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 315, 706) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1628) [M::mem_pestat] mean and std.dev: (413.13, 390.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2089) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 304, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936) [M::mem_pestat] mean and std.dev: (331.05, 168.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1183) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (607, 1680, 4733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12985) [M::mem_pestat] mean and std.dev: (2335.64, 2170.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17111) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 250, 658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1578) [M::mem_pestat] mean and std.dev: (318.41, 192.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2038) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689792 sequences (100000105 bp) in 3.210 CPU sec, 3.327 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 280704, 11, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 363, 585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1375) [M::mem_pestat] mean and std.dev: (390.00, 292.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 303, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (331.12, 170.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1190) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (162, 402, 695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1761) [M::mem_pestat] mean and std.dev: (523.09, 503.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2538) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 294, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (286.24, 143.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686070 reads in 182.576 CPU sec, 5.798 real sec [M::process] read 694124 sequences (100000026 bp) in 2.165 CPU sec, 2.210 real sec... [M::mem_process_seqs] Processed 686542 reads in 128.364 CPU sec, 4.034 real sec sorting block 140, #reads = 1068334 mem = 536879483 peak = 1114210304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 281085, 6, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 392, 632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1486) [M::mem_pestat] mean and std.dev: (454.19, 330.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1913) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 285, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (314.82, 163.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1136) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 282, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (293.11, 121.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1065) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 141, #reads = 1066277 mem = 536879445 peak = 1114210304 [M::process] read 693096 sequences (100000055 bp) in 2.910 CPU sec, 3.113 real sec... [M::process] read 691790 sequences (100000216 bp) in 1.730 CPU sec, 1.734 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282748, 11, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 315, 542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1272) [M::mem_pestat] mean and std.dev: (285.14, 156.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 266, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (294.89, 152.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1057) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (80, 527, 1211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3473) [M::mem_pestat] mean and std.dev: (627.50, 592.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4604) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 375, 717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1669) [M::mem_pestat] mean and std.dev: (405.03, 263.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2145) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689792 reads in 197.715 CPU sec, 6.288 real sec [M::mem_process_seqs] Processed 694124 reads in 126.472 CPU sec, 4.019 real sec sorting block 142, #reads = 1060886 mem = 536878912 peak = 1114210304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282708, 30, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 354, 742) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1868) [M::mem_pestat] mean and std.dev: (512.39, 493.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 272, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (299.90, 154.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (393, 878, 1745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4449) [M::mem_pestat] mean and std.dev: (1257.83, 1084.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5801) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 257, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (254.74, 122.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691100 sequences (100000131 bp) in 2.875 CPU sec, 3.051 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 282238, 11, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 293, 646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1506) [M::mem_pestat] mean and std.dev: (339.19, 265.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1936) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 276, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (302.38, 154.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1078) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (160, 397, 1529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4267) [M::mem_pestat] mean and std.dev: (630.64, 595.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5636) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 278, 557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1297) [M::mem_pestat] mean and std.dev: (358.87, 277.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1667) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 690796 sequences (100000007 bp) in 1.760 CPU sec, 1.766 real sec... [M::mem_process_seqs] Processed 693096 reads in 164.177 CPU sec, 5.194 real sec [M::mem_process_seqs] Processed 691790 reads in 112.098 CPU sec, 3.559 real sec sorting block 143, #reads = 1074554 mem = 536879454 peak = 1114210304 [M::process] read 690856 sequences (100000206 bp) in 3.158 CPU sec, 3.347 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 282005, 13, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 327, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1215) [M::mem_pestat] mean and std.dev: (343.54, 216.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 282, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (307.16, 155.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1097) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (95, 168, 757) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2081) [M::mem_pestat] mean and std.dev: (250.50, 237.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2743) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 307, 667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1595) [M::mem_pestat] mean and std.dev: (456.19, 326.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2059) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690796 reads in 96.450 CPU sec, 3.032 real sec sorting block 144, #reads = 1073576 mem = 536880661 peak = 1114210304 [M::process] read 690508 sequences (100000257 bp) in 2.949 CPU sec, 3.094 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282452, 4, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 222, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (223.64, 87.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 280, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (305.00, 154.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1081) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 319, 645) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1455) [M::mem_pestat] mean and std.dev: (408.37, 299.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1860) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 281785, 25, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 254, 632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1486) [M::mem_pestat] mean and std.dev: (469.04, 347.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1913) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 281, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (305.94, 154.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1089) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (389, 600, 1203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2831) [M::mem_pestat] mean and std.dev: (655.09, 587.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3645) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 297, 708) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1652) [M::mem_pestat] mean and std.dev: (363.67, 288.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2124) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691100 reads in 265.666 CPU sec, 8.412 real sec [M::mem_process_seqs] Processed 690856 reads in 139.235 CPU sec, 4.407 real sec [M::process] read 690434 sequences (100000030 bp) in 3.307 CPU sec, 3.514 real sec... sorting block 145, #reads = 1073660 mem = 536876935 peak = 1114210304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 281995, 11, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 439, 1210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3170) [M::mem_pestat] mean and std.dev: (789.62, 760.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4150) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 283, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (307.68, 154.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (538, 747, 9533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 27523) [M::mem_pestat] mean and std.dev: (3650.45, 4225.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 36518) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 285, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1196) [M::mem_pestat] mean and std.dev: (329.30, 250.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1530) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 281626, 8, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (238, 366, 1023) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2593) [M::mem_pestat] mean and std.dev: (582.55, 478.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3378) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 283, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (308.12, 155.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (250, 422, 1481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3943) [M::mem_pestat] mean and std.dev: (767.65, 1023.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5174) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689864 sequences (100000109 bp) in 3.313 CPU sec, 3.548 real sec... [M::mem_process_seqs] Processed 690508 reads in 178.680 CPU sec, 5.704 real sec [M::mem_process_seqs] Processed 690434 reads in 125.061 CPU sec, 3.953 real sec sorting block 146, #reads = 1073088 mem = 536877702 peak = 1114210304 [M::process] read 689142 sequences (100000095 bp) in 2.997 CPU sec, 3.150 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 281530, 6, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 269, 718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1834) [M::mem_pestat] mean and std.dev: (415.00, 337.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2392) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 288, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (312.62, 158.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 245, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974) [M::mem_pestat] mean and std.dev: (315.92, 201.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1228) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 281305, 9, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 251, 986) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2670) [M::mem_pestat] mean and std.dev: (522.13, 598.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 290, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (313.75, 157.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 354, 1442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3988) [M::mem_pestat] mean and std.dev: (689.05, 625.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5261) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689378 sequences (100000218 bp) in 2.842 CPU sec, 3.000 real sec... [M::mem_process_seqs] Processed 689864 reads in 177.014 CPU sec, 5.627 real sec sorting block 147, #reads = 1072154 mem = 536880546 peak = 1114210304 [M::mem_process_seqs] Processed 689142 reads in 124.473 CPU sec, 3.925 real sec [M::process] read 689120 sequences (100000017 bp) in 3.026 CPU sec, 3.264 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 281208, 8, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 352, 625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1543) [M::mem_pestat] mean and std.dev: (385.32, 250.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2002) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 290, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (314.95, 159.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 362, 1427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3821) [M::mem_pestat] mean and std.dev: (740.32, 574.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5018) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 148, #reads = 1072671 mem = 536881374 peak = 1114210304 [M::process] read 689470 sequences (100000150 bp) in 3.107 CPU sec, 3.304 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 281067, 16, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (240, 379, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029) [M::mem_pestat] mean and std.dev: (364.00, 196.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1292) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 290, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (315.56, 159.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (479, 898, 898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1736) [M::mem_pestat] mean and std.dev: (672.40, 254.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2155) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 292, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1148) [M::mem_pestat] mean and std.dev: (396.95, 281.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1525) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689378 reads in 189.433 CPU sec, 6.033 real sec [M::mem_process_seqs] Processed 689120 reads in 134.077 CPU sec, 4.237 real sec [M::process] read 689042 sequences (100000242 bp) in 2.786 CPU sec, 2.969 real sec... sorting block 149, #reads = 1072506 mem = 536885540 peak = 1114210304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 281106, 18, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 304, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (272.12, 116.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 290, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (314.81, 159.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (143, 1518, 2408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6938) [M::mem_pestat] mean and std.dev: (1340.17, 907.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9203) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 291, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (272.67, 84.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1016) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688858 sequences (100000147 bp) in 3.257 CPU sec, 3.465 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 281378, 7, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 304, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (268.98, 98.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 290, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (314.55, 157.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 328, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (274.46, 106.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689470 reads in 191.461 CPU sec, 6.083 real sec [M::mem_process_seqs] Processed 689042 reads in 131.599 CPU sec, 4.147 real sec [M::process] read 688404 sequences (100000260 bp) in 2.340 CPU sec, 2.460 real sec... sorting block 150, #reads = 1070024 mem = 536878661 peak = 1114210304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 281066, 7, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 377, 980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2522) [M::mem_pestat] mean and std.dev: (467.00, 420.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3293) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 292, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (316.44, 159.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 355, 741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1785) [M::mem_pestat] mean and std.dev: (447.40, 353.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2307) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688158 sequences (100000058 bp) in 2.711 CPU sec, 2.887 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 280668, 8, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 254, 1132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2986) [M::mem_pestat] mean and std.dev: (582.67, 561.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3913) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 294, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (318.61, 160.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (337, 403, 975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2251) [M::mem_pestat] mean and std.dev: (478.17, 255.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2889) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687956 sequences (100000159 bp) in 1.600 CPU sec, 1.605 real sec... [M::mem_process_seqs] Processed 688858 reads in 181.205 CPU sec, 5.769 real sec sorting block 151, #reads = 1070037 mem = 536880666 peak = 1114210304 [M::mem_process_seqs] Processed 688404 reads in 122.739 CPU sec, 3.888 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 281207, 9, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 266, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1208) [M::mem_pestat] mean and std.dev: (305.00, 197.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 295, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (319.61, 160.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 274, 1230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3310) [M::mem_pestat] mean and std.dev: (760.48, 793.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4350) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 152, #reads = 1070325 mem = 536875013 peak = 1114210304 [M::process] read 687606 sequences (100000270 bp) in 2.505 CPU sec, 2.619 real sec... [M::process] read 687010 sequences (100000255 bp) in 1.780 CPU sec, 1.781 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 280802, 7, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 350, 672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1670) [M::mem_pestat] mean and std.dev: (344.15, 207.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2169) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 297, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (320.02, 160.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 301, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (265.10, 94.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688158 reads in 171.467 CPU sec, 5.442 real sec [M::mem_process_seqs] Processed 687956 reads in 111.888 CPU sec, 3.559 real sec sorting block 153, #reads = 1071127 mem = 536882368 peak = 1114210304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 280453, 9, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 268, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966) [M::mem_pestat] mean and std.dev: (320.78, 235.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1264) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 299, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (321.78, 160.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1151) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 261, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (262.68, 84.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686984 sequences (100000183 bp) in 2.543 CPU sec, 2.702 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 279903, 3, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (243, 309, 1154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2976) [M::mem_pestat] mean and std.dev: (644.04, 662.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3887) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 300, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (322.90, 160.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 223, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956) [M::mem_pestat] mean and std.dev: (267.64, 152.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1213) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687168 sequences (100000114 bp) in 1.710 CPU sec, 1.712 real sec... [M::mem_process_seqs] Processed 687606 reads in 165.550 CPU sec, 5.256 real sec [M::mem_process_seqs] Processed 687010 reads in 119.808 CPU sec, 3.818 real sec sorting block 154, #reads = 1068306 mem = 536876738 peak = 1114210304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 279715, 7, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 413, 660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1650) [M::mem_pestat] mean and std.dev: (376.28, 289.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2145) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 300, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (322.81, 160.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 281, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018) [M::mem_pestat] mean and std.dev: (272.53, 144.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1298) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686912 sequences (100000110 bp) in 2.471 CPU sec, 2.641 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 280219, 11, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 312, 735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1883) [M::mem_pestat] mean and std.dev: (395.68, 346.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 299, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (322.13, 160.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (573, 727, 1237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2565) [M::mem_pestat] mean and std.dev: (667.50, 361.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3229) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 339, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (271.05, 97.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687116 sequences (100000001 bp) in 1.790 CPU sec, 1.798 real sec... [M::mem_process_seqs] Processed 686984 reads in 159.087 CPU sec, 5.037 real sec sorting block 155, #reads = 1068149 mem = 536877988 peak = 1114210304 [M::mem_process_seqs] Processed 687168 reads in 108.353 CPU sec, 3.418 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 280162, 17, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 276, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (245.58, 85.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 299, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (322.73, 161.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (741, 1317, 1884) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4170) [M::mem_pestat] mean and std.dev: (1339.75, 522.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5313) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 275, 1435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3913) [M::mem_pestat] mean and std.dev: (677.54, 852.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5152) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687222 sequences (100000167 bp) in 2.743 CPU sec, 2.846 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 280055, 11, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (249, 300, 570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1212) [M::mem_pestat] mean and std.dev: (328.23, 195.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 298, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (322.13, 161.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (74, 415, 2168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6356) [M::mem_pestat] mean and std.dev: (963.55, 1301.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8450) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 300, 592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1324) [M::mem_pestat] mean and std.dev: (348.66, 232.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1690) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687268 sequences (100000119 bp) in 1.910 CPU sec, 1.914 real sec... [M::mem_process_seqs] Processed 686912 reads in 158.526 CPU sec, 5.005 real sec sorting block 156, #reads = 1068779 mem = 536882803 peak = 1114210304 [M::mem_process_seqs] Processed 687116 reads in 108.386 CPU sec, 3.423 real sec sorting block 157, #reads = 1069309 mem = 536878670 peak = 1114210304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 279980, 15, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 277, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (295.71, 133.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1009) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 298, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (321.03, 159.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1136) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (112, 408, 1033) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2875) [M::mem_pestat] mean and std.dev: (596.40, 528.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3796) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 376, 1722) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4806) [M::mem_pestat] mean and std.dev: (733.06, 775.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6348) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687078 sequences (100000004 bp) in 2.529 CPU sec, 2.674 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 280253, 14, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 235, 564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1344) [M::mem_pestat] mean and std.dev: (380.23, 333.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 298, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (321.88, 161.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (99, 525, 815) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2247) [M::mem_pestat] mean and std.dev: (567.79, 520.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2963) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 228, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (255.36, 123.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686682 sequences (100000034 bp) in 1.760 CPU sec, 1.756 real sec... [M::mem_process_seqs] Processed 687222 reads in 166.498 CPU sec, 5.466 real sec [M::mem_process_seqs] Processed 687268 reads in 109.974 CPU sec, 3.475 real sec sorting block 158, #reads = 1067665 mem = 536878748 peak = 1114210304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 280208, 12, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 279, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (280.03, 134.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1190) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 296, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (321.41, 161.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (291, 1024, 1181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2961) [M::mem_pestat] mean and std.dev: (793.42, 688.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3851) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 320, 779) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1899) [M::mem_pestat] mean and std.dev: (418.24, 297.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2459) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686790 sequences (100000280 bp) in 2.610 CPU sec, 2.761 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 280417, 8, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 251, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799) [M::mem_pestat] mean and std.dev: (247.53, 111.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1006) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 298, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (321.54, 160.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1136) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 246, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (302.86, 175.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686818 sequences (100000127 bp) in 1.940 CPU sec, 1.939 real sec... [M::mem_process_seqs] Processed 687078 reads in 168.524 CPU sec, 5.335 real sec sorting block 159, #reads = 1067239 mem = 536881081 peak = 1114210304 [M::process] read 686588 sequences (100000176 bp) in 2.482 CPU sec, 2.486 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 280084, 24, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 341, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1061) [M::mem_pestat] mean and std.dev: (316.48, 194.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1362) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 298, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (322.58, 161.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (293, 676, 2663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7403) [M::mem_pestat] mean and std.dev: (1238.21, 1461.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9773) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (245, 312, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1073) [M::mem_pestat] mean and std.dev: (345.33, 216.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1349) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686818 reads in 104.515 CPU sec, 3.305 real sec [M::mem_process_seqs] Processed 686682 reads in 248.750 CPU sec, 7.888 real sec sorting block 160, #reads = 1065946 mem = 536881572 peak = 1114210304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 280156, 12, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (247, 352, 792) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1882) [M::mem_pestat] mean and std.dev: (569.48, 462.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 297, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (321.86, 160.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (607, 1234, 3910) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10516) [M::mem_pestat] mean and std.dev: (1545.08, 1429.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13819) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (263, 323, 1051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2627) [M::mem_pestat] mean and std.dev: (568.13, 447.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3415) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687072 sequences (100000018 bp) in 2.331 CPU sec, 2.627 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 279929, 28, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 281, 580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1290) [M::mem_pestat] mean and std.dev: (347.31, 175.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 300, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (323.69, 161.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (879, 1516, 3295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8127) [M::mem_pestat] mean and std.dev: (1545.50, 1146.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10543) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 227, 859) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2297) [M::mem_pestat] mean and std.dev: (449.29, 461.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3016) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686790 reads in 247.596 CPU sec, 7.829 real sec [M::mem_process_seqs] Processed 686588 reads in 109.464 CPU sec, 3.439 real sec [M::process] read 686960 sequences (100000132 bp) in 2.199 CPU sec, 2.252 real sec... sorting block 161, #reads = 1065818 mem = 536886310 peak = 1114210304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 279702, 9, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 323, 615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1315) [M::mem_pestat] mean and std.dev: (341.13, 208.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 298, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (323.51, 162.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 341, 880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2214) [M::mem_pestat] mean and std.dev: (540.20, 508.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2881) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687012 sequences (100000016 bp) in 2.360 CPU sec, 2.501 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 280112, 10, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 284, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (269.00, 115.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1083) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 299, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (324.58, 163.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (508, 2006, 3849) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10531) [M::mem_pestat] mean and std.dev: (2784.20, 2832.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14116) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (248, 478, 785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1859) [M::mem_pestat] mean and std.dev: (505.91, 335.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2396) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693064 sequences (100000195 bp) in 1.750 CPU sec, 1.754 real sec... [M::mem_process_seqs] Processed 687072 reads in 165.951 CPU sec, 5.265 real sec sorting block 162, #reads = 1066175 mem = 536878956 peak = 1114210304 [M::mem_process_seqs] Processed 686960 reads in 114.997 CPU sec, 3.632 real sec sorting block 163, #reads = 1064656 mem = 536881109 peak = 1114210304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 280019, 13, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (238, 468, 750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1774) [M::mem_pestat] mean and std.dev: (480.29, 280.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2286) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 298, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921) [M::mem_pestat] mean and std.dev: (324.97, 164.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1164) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1022, 1221, 2524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5528) [M::mem_pestat] mean and std.dev: (1718.15, 1192.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7030) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 306, 512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1104) [M::mem_pestat] mean and std.dev: (360.41, 212.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1400) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697174 sequences (100000280 bp) in 2.575 CPU sec, 2.691 real sec... [M::process] read 695384 sequences (100000215 bp) in 1.750 CPU sec, 1.752 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 281896, 15, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 248, 940) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2526) [M::mem_pestat] mean and std.dev: (573.07, 650.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3319) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 269, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844) [M::mem_pestat] mean and std.dev: (298.51, 154.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1067) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (118, 408, 2730) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7954) [M::mem_pestat] mean and std.dev: (902.57, 1067.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10566) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (137, 226, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043) [M::mem_pestat] mean and std.dev: (260.14, 161.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1345) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687012 reads in 187.609 CPU sec, 5.963 real sec [M::mem_process_seqs] Processed 693064 reads in 136.400 CPU sec, 4.355 real sec sorting block 164, #reads = 1056913 mem = 536879352 peak = 1114210304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 283858, 2, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 270, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (241.65, 99.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 252, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (281.71, 145.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 998) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (256, 388, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (355.52, 108.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694356 sequences (100000054 bp) in 2.565 CPU sec, 2.656 real sec... [M::process] read 693594 sequences (100000207 bp) in 1.750 CPU sec, 1.753 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 283503, 12, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 292, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1241) [M::mem_pestat] mean and std.dev: (347.64, 197.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 260, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (289.03, 148.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1030) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (7, 291, 3517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10537) [M::mem_pestat] mean and std.dev: (1072.92, 1495.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14047) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 232, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784) [M::mem_pestat] mean and std.dev: (262.41, 117.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697174 reads in 169.466 CPU sec, 5.365 real sec [M::mem_process_seqs] Processed 695384 reads in 191.685 CPU sec, 6.091 real sec sorting block 165, #reads = 1067441 mem = 536881159 peak = 1114210304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283600, 10, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 316, 1745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4959) [M::mem_pestat] mean and std.dev: (650.91, 773.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 264, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (292.27, 149.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (634, 634, 789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (324, 1099) [M::mem_pestat] mean and std.dev: (730.43, 137.73) [M::mem_pestat] low and high boundaries for proper pairs: (169, 1281) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 269, 707) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1697) [M::mem_pestat] mean and std.dev: (444.50, 326.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2192) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692744 sequences (100000097 bp) in 2.814 CPU sec, 3.005 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 283044, 22, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 263, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972) [M::mem_pestat] mean and std.dev: (293.63, 172.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1234) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 268, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (295.16, 150.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (585, 864, 1398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3024) [M::mem_pestat] mean and std.dev: (837.30, 479.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3837) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (258, 379, 642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1410) [M::mem_pestat] mean and std.dev: (473.80, 331.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1799) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693942 sequences (100000028 bp) in 1.850 CPU sec, 1.857 real sec... [M::mem_process_seqs] Processed 694356 reads in 175.354 CPU sec, 5.555 real sec sorting block 166, #reads = 1069765 mem = 536882580 peak = 1114210304 [M::mem_process_seqs] Processed 693594 reads in 119.039 CPU sec, 3.763 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282917, 16, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 234, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (267.90, 201.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 271, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (298.21, 151.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1064) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (301, 616, 1156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2866) [M::mem_pestat] mean and std.dev: (682.12, 389.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3721) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 352, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075) [M::mem_pestat] mean and std.dev: (339.04, 195.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1369) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694702 sequences (100000226 bp) in 2.688 CPU sec, 2.844 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 283366, 3, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 330, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093) [M::mem_pestat] mean and std.dev: (324.62, 181.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 265, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (293.51, 150.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 237, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006) [M::mem_pestat] mean and std.dev: (247.04, 104.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1282) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694152 sequences (100000173 bp) in 2.040 CPU sec, 2.036 real sec... [M::mem_process_seqs] Processed 692744 reads in 177.221 CPU sec, 5.897 real sec sorting block 167, #reads = 1069453 mem = 536878650 peak = 1114210304 [M::mem_process_seqs] Processed 693942 reads in 115.551 CPU sec, 3.657 real sec sorting block 168, #reads = 1070389 mem = 536879366 peak = 1114210304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 283350, 13, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 285, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (274.83, 135.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 262, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (290.07, 148.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (229, 1363, 6994) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 20524) [M::mem_pestat] mean and std.dev: (2423.69, 2676.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 27289) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 251, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (244.15, 126.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692400 sequences (100000012 bp) in 2.942 CPU sec, 3.121 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282741, 5, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 267, 782) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1886) [M::mem_pestat] mean and std.dev: (411.66, 343.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 265, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (293.12, 149.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (306, 556, 1098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2682) [M::mem_pestat] mean and std.dev: (732.72, 664.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3474) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693154 sequences (100000109 bp) in 1.820 CPU sec, 1.825 real sec... [M::mem_process_seqs] Processed 694702 reads in 182.616 CPU sec, 5.783 real sec [M::mem_process_seqs] Processed 694152 reads in 123.293 CPU sec, 3.902 real sec sorting block 169, #reads = 1066739 mem = 536878517 peak = 1114210304 [M::process] read 690996 sequences (100000242 bp) in 3.116 CPU sec, 3.287 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282526, 4, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (261, 416, 714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1620) [M::mem_pestat] mean and std.dev: (447.16, 312.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2073) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 268, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (296.18, 152.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 303, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (309.39, 135.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 690650 sequences (100000129 bp) in 1.899 CPU sec, 1.908 real sec... [M::mem_process_seqs] Processed 693154 reads in 101.322 CPU sec, 3.197 real sec sorting block 170, #reads = 1066948 mem = 536877608 peak = 1114210304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282484, 15, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 343, 1299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3485) [M::mem_pestat] mean and std.dev: (608.48, 759.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 273, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (301.42, 154.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1085) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1221, 1758, 5148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13002) [M::mem_pestat] mean and std.dev: (2701.07, 1845.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16929) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (238, 388, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (299.86, 85.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282080, 10, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 268, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (333.59, 218.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1209) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 277, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (306.04, 156.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1100) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (576, 1045, 2575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6573) [M::mem_pestat] mean and std.dev: (1533.70, 1426.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8572) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 330, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (329.65, 141.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692400 reads in 268.383 CPU sec, 8.490 real sec [M::process] read 690848 sequences (100000185 bp) in 2.254 CPU sec, 2.259 real sec... [M::mem_process_seqs] Processed 690996 reads in 139.382 CPU sec, 4.424 real sec sorting block 171, #reads = 1069430 mem = 536880181 peak = 1114210304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282404, 4, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 328, 981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2533) [M::mem_pestat] mean and std.dev: (434.00, 333.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3309) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 281, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (308.35, 156.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 292, 568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1230) [M::mem_pestat] mean and std.dev: (363.75, 172.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1561) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689766 sequences (100000117 bp) in 2.775 CPU sec, 2.937 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282084, 16, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (254, 329, 631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1385) [M::mem_pestat] mean and std.dev: (448.15, 264.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1762) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 280, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (307.52, 156.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1009, 2625, 4146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10420) [M::mem_pestat] mean and std.dev: (3453.62, 3160.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16097) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (261, 443, 1638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4392) [M::mem_pestat] mean and std.dev: (625.36, 655.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5769) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689450 sequences (100000164 bp) in 1.960 CPU sec, 1.960 real sec... [M::mem_process_seqs] Processed 690650 reads in 173.221 CPU sec, 5.483 real sec sorting block 172, #reads = 1068705 mem = 536880090 peak = 1114210304 [M::mem_process_seqs] Processed 690848 reads in 117.100 CPU sec, 3.705 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 281387, 13, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 369, 1053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2747) [M::mem_pestat] mean and std.dev: (657.93, 569.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 285, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (312.85, 159.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (528, 1886, 4001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10947) [M::mem_pestat] mean and std.dev: (2444.85, 2013.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14420) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 247, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (246.75, 89.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688840 sequences (100000130 bp) in 2.820 CPU sec, 2.957 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 281361, 3, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 319, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1232) [M::mem_pestat] mean and std.dev: (323.32, 160.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 289, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (315.29, 159.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 296, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (272.57, 117.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688438 sequences (100000117 bp) in 2.010 CPU sec, 2.007 real sec... [M::mem_process_seqs] Processed 689766 reads in 169.782 CPU sec, 5.360 real sec sorting block 173, #reads = 1066192 mem = 536881792 peak = 1114210304 [M::mem_process_seqs] Processed 689450 reads in 192.297 CPU sec, 6.091 real sec sorting block 174, #reads = 1067143 mem = 536884516 peak = 1114210304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 281392, 9, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 226, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1156) [M::mem_pestat] mean and std.dev: (276.38, 196.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 291, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (316.88, 159.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1143) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 247, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (275.04, 126.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687914 sequences (100000195 bp) in 3.018 CPU sec, 3.122 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 280946, 10, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 390, 515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1131) [M::mem_pestat] mean and std.dev: (368.44, 196.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 291, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (316.54, 158.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1136) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (548, 1247, 1518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3458) [M::mem_pestat] mean and std.dev: (941.20, 511.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4428) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 328, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (282.25, 105.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1079) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687600 sequences (100000212 bp) in 1.880 CPU sec, 1.877 real sec... [M::mem_process_seqs] Processed 688840 reads in 171.008 CPU sec, 5.404 real sec [M::mem_process_seqs] Processed 688438 reads in 113.868 CPU sec, 3.596 real sec sorting block 175, #reads = 1066856 mem = 536881100 peak = 1114210304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 280177, 12, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 330, 779) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2009) [M::mem_pestat] mean and std.dev: (415.52, 347.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 294, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (317.98, 158.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (374, 954, 2084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5504) [M::mem_pestat] mean and std.dev: (972.58, 718.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7214) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 428, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1082) [M::mem_pestat] mean and std.dev: (425.17, 264.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1481) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687642 sequences (100000108 bp) in 2.747 CPU sec, 2.952 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 280558, 13, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 371, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1106) [M::mem_pestat] mean and std.dev: (346.07, 195.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 295, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (319.20, 158.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1138) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (233, 871, 1379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3671) [M::mem_pestat] mean and std.dev: (778.67, 742.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4817) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 265, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (281.19, 80.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687326 sequences (100000170 bp) in 1.750 CPU sec, 1.755 real sec... [M::mem_process_seqs] Processed 687914 reads in 175.451 CPU sec, 5.586 real sec [M::mem_process_seqs] Processed 687600 reads in 117.671 CPU sec, 3.724 real sec sorting block 176, #reads = 1066259 mem = 536879384 peak = 1114210304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 280641, 17, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 335, 942) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2410) [M::mem_pestat] mean and std.dev: (549.42, 568.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3144) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 295, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (319.53, 160.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1142) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (332, 708, 1122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2702) [M::mem_pestat] mean and std.dev: (786.50, 648.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3492) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 213, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (235.03, 102.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687550 sequences (100000201 bp) in 2.593 CPU sec, 2.701 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 280215, 18, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 315, 551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1279) [M::mem_pestat] mean and std.dev: (394.43, 250.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 297, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (321.37, 161.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (336, 816, 1264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3120) [M::mem_pestat] mean and std.dev: (742.12, 516.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4048) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 276, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (272.33, 124.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1074) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686824 sequences (100000227 bp) in 1.960 CPU sec, 1.957 real sec... [M::mem_process_seqs] Processed 687642 reads in 163.425 CPU sec, 5.164 real sec sorting block 177, #reads = 1065321 mem = 536882952 peak = 1114210304 [M::mem_process_seqs] Processed 687326 reads in 111.041 CPU sec, 3.510 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 280395, 18, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 319, 506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1206) [M::mem_pestat] mean and std.dev: (335.44, 207.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 293, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (319.91, 161.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (198, 490, 1593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4383) [M::mem_pestat] mean and std.dev: (805.28, 773.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5778) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (270, 436, 638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1374) [M::mem_pestat] mean and std.dev: (500.21, 320.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1783) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 178, #reads = 1068330 mem = 536877292 peak = 1114210304 [M::process] read 686628 sequences (100000282 bp) in 2.753 CPU sec, 2.953 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 280463, 8, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 368, 642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1520) [M::mem_pestat] mean and std.dev: (358.60, 200.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1959) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 298, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (323.58, 162.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (260, 298, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (38, 593) [M::mem_pestat] mean and std.dev: (291.44, 89.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686506 sequences (100000225 bp) in 1.750 CPU sec, 1.750 real sec... [M::mem_process_seqs] Processed 687550 reads in 162.350 CPU sec, 5.137 real sec [M::mem_process_seqs] Processed 686824 reads in 111.390 CPU sec, 3.516 real sec sorting block 179, #reads = 1068125 mem = 536878566 peak = 1114210304 [M::process] read 686710 sequences (100000183 bp) in 2.676 CPU sec, 2.861 real sec... [M::process] read 685988 sequences (100000093 bp) in 1.660 CPU sec, 1.659 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 280037, 11, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 495, 554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1308) [M::mem_pestat] mean and std.dev: (396.50, 263.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 300, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (323.47, 161.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 123, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1505) [M::mem_pestat] mean and std.dev: (358.91, 441.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2126) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 364, 853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2155) [M::mem_pestat] mean and std.dev: (476.60, 324.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2806) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686710 reads in 85.856 CPU sec, 2.942 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 280027, 14, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 272, 636) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1652) [M::mem_pestat] mean and std.dev: (331.65, 263.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2160) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 300, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (323.08, 160.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1138) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (119, 763, 930) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2552) [M::mem_pestat] mean and std.dev: (572.23, 603.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3363) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 264, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (269.19, 106.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 180, #reads = 1068372 mem = 536881111 peak = 1114210304 [M::mem_process_seqs] Processed 686506 reads in 175.472 CPU sec, 6.310 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 280580, 8, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 273, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1159) [M::mem_pestat] mean and std.dev: (267.26, 183.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 298, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (321.58, 159.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (328, 391, 542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970) [M::mem_pestat] mean and std.dev: (382.50, 143.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1184) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 279968, 8, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 235, 1294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3548) [M::mem_pestat] mean and std.dev: (383.16, 389.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 685894 sequences (100000275 bp) in 2.703 CPU sec, 2.905 real sec... [M::mem_pestat] (25, 50, 75) percentile: (195, 302, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (325.31, 161.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 244, 990) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2600) [M::mem_pestat] mean and std.dev: (490.77, 520.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3405) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686628 reads in 335.124 CPU sec, 11.373 real sec [M::mem_process_seqs] Processed 685988 reads in 128.656 CPU sec, 4.101 real sec sorting block 181, #reads = 1068982 mem = 536881006 peak = 1114210304 [M::process] read 685688 sequences (100000174 bp) in 2.610 CPU sec, 2.839 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 280058, 14, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 338, 632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1572) [M::mem_pestat] mean and std.dev: (321.29, 208.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2042) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 302, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (325.70, 161.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (246, 1344, 2914) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8250) [M::mem_pestat] mean and std.dev: (1995.21, 2361.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11441) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 286, 758) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1868) [M::mem_pestat] mean and std.dev: (436.76, 397.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2423) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686028 sequences (100000287 bp) in 2.470 CPU sec, 2.636 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 280228, 7, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 217, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (203.08, 68.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 305, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (327.84, 162.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (245, 347, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031) [M::mem_pestat] mean and std.dev: (330.95, 135.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1293) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685894 reads in 159.127 CPU sec, 5.069 real sec sorting block 182, #reads = 1068023 mem = 536879203 peak = 1114210304 [M::mem_process_seqs] Processed 685688 reads in 109.891 CPU sec, 3.462 real sec [M::process] read 685432 sequences (100000229 bp) in 2.500 CPU sec, 2.670 real sec... sorting block 183, #reads = 1068913 mem = 536876861 peak = 1114210304 [M::process] read 686288 sequences (100000237 bp) in 2.615 CPU sec, 2.830 real sec... [M::process] read 49928 sequences (7243175 bp) in 0.330 CPU sec, 0.331 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 279953, 15, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 408, 708) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1652) [M::mem_pestat] mean and std.dev: (430.82, 309.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2124) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 305, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923) [M::mem_pestat] mean and std.dev: (329.20, 164.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1165) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (205, 372, 987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2551) [M::mem_pestat] mean and std.dev: (536.07, 511.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3333) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 357, 752) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1900) [M::mem_pestat] mean and std.dev: (338.72, 224.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2474) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685432 reads in 91.460 CPU sec, 2.920 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 279988, 19, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 230, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994) [M::mem_pestat] mean and std.dev: (276.84, 160.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1274) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 305, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (328.35, 164.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1164) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (127, 243, 709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1873) [M::mem_pestat] mean and std.dev: (419.17, 418.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2455) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 258, 609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1463) [M::mem_pestat] mean and std.dev: (329.84, 212.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1890) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 280243, 14, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 368, 600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1392) [M::mem_pestat] mean and std.dev: (367.33, 197.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1788) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 301, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (326.46, 164.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1159) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (193, 614, 870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2224) [M::mem_pestat] mean and std.dev: (648.93, 566.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2915) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 276, 583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1373) [M::mem_pestat] mean and std.dev: (325.59, 211.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1768) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686028 reads in 261.772 CPU sec, 8.425 real sec sorting block 184, #reads = 1067873 mem = 536880212 peak = 1114210304 [M::mem_process_seqs] Processed 686288 reads in 144.741 CPU sec, 4.710 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 20402, 1, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 286, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (309.44, 154.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1085) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 49928 reads in 8.475 CPU sec, 0.298 real sec sorting block 185, #reads = 1068944 mem = 536879604 peak = 1114210304 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: 1f8bfbff fffab20b extended: f1bf07ab 1a407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8559C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 198130662 overall: 70090809344 mem 32278.195 user 733.368 sys 1121.131 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1029_I9B-T1-TDNA-1_A23MHWWLT4_1.L002\tPU:L002\tSM:1029_I9B-T1-TDNA-1_A23MHWWLT4_1\tLB:1029_I9B-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1029_I9B-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 1029_I9B-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 1121.160 sec; CPU: 33011.566 sec sorting block 186, #reads = 233167 mem = 118291932 peak = 1114210304 uniq header count 1 merging 187 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: 1f8bfbff fffab20b extended: f1bf07ab 1a407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8559C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 10976368354 output reads: 199374214 bam_mem_sort: 187 calls 111.318 user 3.278 sys 115.470 real bam_write: 187 calls 58.446 user 3.759 sys 75.051 real execute: 1 calls 42.837 user 3.063 sys 1196.536 real merge_files: 1 calls 42.777 user 3.049 sys 75.227 real parse_chunk: 2980 calls 168.508 user 40.580 sys 216.655 real read_chunk: 8049 calls 91.315 user 58.657 sys 1044.388 real sort_block: 1 calls 63.502 user 57.608 sys 1007.178 real write_chunk: 5067 calls 42.430 user 2.541 sys 74.030 real overall: 3690151936 mem 3017.155 user 146.979 sys 1196.555 real ls: cannot access '*.cram': No such file or directory