cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1029_FZX-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2206.046 CPU sec, 84.802 real sec [M::process] read 686540 sequences (100000005 bp) in 1.023 CPU sec, 1.024 real sec... [M::process] read 684352 sequences (100000062 bp) in 1.677 CPU sec, 1.882 real sec... [M::process] read 683844 sequences (100000000 bp) in 1.650 CPU sec, 1.739 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 279722, 3, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 230, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (209.33, 74.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 305, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (326.75, 161.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 241, 787) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2051) [M::mem_pestat] mean and std.dev: (455.89, 473.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2683) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683352 sequences (100000049 bp) in 1.613 CPU sec, 1.728 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 278170, 15, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 326, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (290.51, 121.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 322, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (339.65, 164.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1176) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (188, 521, 947) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2465) [M::mem_pestat] mean and std.dev: (550.71, 423.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3224) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 298, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (298.41, 105.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 278810, 10, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 319, 688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1634) [M::mem_pestat] mean and std.dev: (317.27, 191.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2107) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 319, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (338.74, 165.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1182) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (738, 964, 2899) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7221) [M::mem_pestat] mean and std.dev: (1555.00, 1149.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9382) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 259, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (255.31, 122.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1104) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686540 reads in 342.910 CPU sec, 11.614 real sec [M::mem_process_seqs] Processed 683844 reads in 237.343 CPU sec, 7.948 real sec [M::mem_process_seqs] Processed 684352 reads in 261.673 CPU sec, 8.808 real sec sorting block 0, #reads = 1070313 mem = 536879118 peak = 580988928 [M::process] read 682858 sequences (100000061 bp) in 2.192 CPU sec, 2.532 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 277960, 20, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 317, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (314.84, 169.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 997) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 327, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943) [M::mem_pestat] mean and std.dev: (343.80, 165.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1187) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (712, 1339, 1363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2665) [M::mem_pestat] mean and std.dev: (944.76, 470.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3316) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 214, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (224.36, 93.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682770 sequences (100000090 bp) in 2.110 CPU sec, 2.369 real sec... [M::process] read 682354 sequences (100000185 bp) in 1.669 CPU sec, 1.676 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 278271, 10, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 190, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (218.27, 120.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1142) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 328, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (344.25, 164.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1181) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (110, 509, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1307) [M::mem_pestat] mean and std.dev: (319.00, 190.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1706) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 294, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (335.32, 182.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1176) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683352 reads in 214.673 CPU sec, 7.076 real sec sorting block 1, #reads = 1069593 mem = 536878595 peak = 849948672 [M::mem_process_seqs] Processed 682858 reads in 142.362 CPU sec, 4.666 real sec sorting block 2, #reads = 1068638 mem = 536878102 peak = 922284032 [M::process] read 681908 sequences (100000205 bp) in 2.006 CPU sec, 2.380 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278123, 1, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 286, 721) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1857) [M::mem_pestat] mean and std.dev: (477.19, 444.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 331, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (346.46, 165.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1190) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 239, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (291.02, 164.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 997) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682028 sequences (100000122 bp) in 1.842 CPU sec, 1.925 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 278403, 7, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 273, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (258.96, 122.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 333, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956) [M::mem_pestat] mean and std.dev: (349.01, 166.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1203) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 286, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042) [M::mem_pestat] mean and std.dev: (358.60, 201.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1313) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682770 reads in 213.197 CPU sec, 7.208 real sec [M::mem_process_seqs] Processed 682354 reads in 142.351 CPU sec, 4.761 real sec sorting block 3, #reads = 1068091 mem = 536878945 peak = 922284032 [M::process] read 681530 sequences (100000119 bp) in 2.195 CPU sec, 2.522 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 278450, 8, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (254, 392, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956) [M::mem_pestat] mean and std.dev: (345.74, 161.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1190) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (218, 337, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965) [M::mem_pestat] mean and std.dev: (352.71, 168.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1214) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 279, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (228.21, 81.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681552 sequences (100000253 bp) in 1.711 CPU sec, 1.765 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 277699, 18, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 226, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (314.84, 214.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1173) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (217, 337, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970) [M::mem_pestat] mean and std.dev: (353.67, 169.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1221) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (515, 552, 552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (441, 626) [M::mem_pestat] mean and std.dev: (532.08, 18.45) [M::mem_pestat] low and high boundaries for proper pairs: (404, 663) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 227, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (269.00, 115.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681908 reads in 214.156 CPU sec, 7.212 real sec [M::mem_process_seqs] Processed 682028 reads in 142.751 CPU sec, 4.777 real sec sorting block 4, #reads = 1068691 mem = 536878512 peak = 922284032 [M::process] read 681128 sequences (100000212 bp) in 2.236 CPU sec, 2.536 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 277274, 3, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 210, 578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1422) [M::mem_pestat] mean and std.dev: (279.30, 186.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1844) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (219, 336, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954) [M::mem_pestat] mean and std.dev: (351.96, 167.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1199) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 352, 602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1334) [M::mem_pestat] mean and std.dev: (368.82, 175.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1700) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680858 sequences (100000227 bp) in 1.673 CPU sec, 1.752 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 277551, 12, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 324, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1084) [M::mem_pestat] mean and std.dev: (335.80, 234.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1399) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (221, 343, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983) [M::mem_pestat] mean and std.dev: (357.64, 170.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1237) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (3, 3, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (44.45, 54.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 266, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1345) [M::mem_pestat] mean and std.dev: (292.57, 159.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1741) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681530 reads in 219.218 CPU sec, 7.328 real sec sorting block 5, #reads = 1068637 mem = 536881402 peak = 922284032 [M::mem_process_seqs] Processed 681552 reads in 143.025 CPU sec, 4.770 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 277503, 12, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 334, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040) [M::mem_pestat] mean and std.dev: (388.42, 227.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1325) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (222, 342, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978) [M::process] read 680726 sequences (100000140 bp) in 2.502 CPU sec, 2.751 real sec... [M::mem_pestat] mean and std.dev: (357.68, 169.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1230) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (196, 335, 647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1549) [M::mem_pestat] mean and std.dev: (314.09, 194.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2000) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 238, 512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1186) [M::mem_pestat] mean and std.dev: (313.56, 164.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1523) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 6, #reads = 1068193 mem = 536881086 peak = 957669376 [M::process] read 680788 sequences (100000232 bp) in 1.792 CPU sec, 1.833 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 277365, 7, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 395, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1253) [M::mem_pestat] mean and std.dev: (326.06, 230.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (223, 344, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979) [M::mem_pestat] mean and std.dev: (359.07, 169.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1231) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 214, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (210.77, 57.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681128 reads in 218.841 CPU sec, 7.415 real sec [M::mem_process_seqs] Processed 680858 reads in 147.369 CPU sec, 4.979 real sec sorting block 7, #reads = 1067173 mem = 536880468 peak = 957669376 [M::process] read 681026 sequences (100000107 bp) in 2.245 CPU sec, 2.553 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 276953, 12, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 426, 851) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2181) [M::mem_pestat] mean and std.dev: (312.87, 185.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2846) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (223, 344, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979) [M::mem_pestat] mean and std.dev: (358.96, 169.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1231) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (79, 450, 641) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1765) [M::mem_pestat] mean and std.dev: (330.36, 252.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2327) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 314, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1231) [M::mem_pestat] mean and std.dev: (312.30, 193.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1582) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680868 sequences (100000111 bp) in 1.678 CPU sec, 1.700 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 277193, 5, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 307, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (293.19, 102.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (223, 344, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979) [M::mem_pestat] mean and std.dev: (359.02, 170.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1231) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (251, 310, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (330.61, 145.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680726 reads in 221.410 CPU sec, 7.537 real sec [M::mem_process_seqs] Processed 680788 reads in 145.231 CPU sec, 4.935 real sec sorting block 8, #reads = 1067042 mem = 536878694 peak = 957669376 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 276635, 11, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 217, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (200.08, 82.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (221, 341, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974) [M::mem_pestat] mean and std.dev: (356.40, 168.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1225) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (98, 98, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1127) [M::mem_pestat] mean and std.dev: (232.10, 268.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1470) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 261, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1097) [M::mem_pestat] mean and std.dev: (339.54, 196.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1399) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680810 sequences (100000082 bp) in 2.737 CPU sec, 3.074 real sec... [M::process] read 680650 sequences (100000246 bp) in 1.770 CPU sec, 1.793 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 276833, 16, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 219, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (215.33, 62.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (222, 340, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966) [M::mem_pestat] mean and std.dev: (355.73, 167.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1214) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (234, 408, 1286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3390) [M::mem_pestat] mean and std.dev: (505.40, 459.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4442) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 290, 882) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2250) [M::mem_pestat] mean and std.dev: (582.62, 605.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3006) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681026 reads in 226.070 CPU sec, 7.628 real sec [M::mem_process_seqs] Processed 680868 reads in 147.396 CPU sec, 5.004 real sec sorting block 9, #reads = 1066871 mem = 536881290 peak = 957669376 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 277249, 6, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 225, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (248.17, 103.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (222, 338, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951) [M::mem_pestat] mean and std.dev: (353.53, 166.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1194) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 299, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988) [M::mem_pestat] mean and std.dev: (357.08, 205.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1253) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680930 sequences (100000171 bp) in 2.374 CPU sec, 2.639 real sec... [M::process] read 681108 sequences (100000243 bp) in 1.760 CPU sec, 1.764 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 277125, 12, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 229, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (228.64, 103.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (221, 337, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947) [M::mem_pestat] mean and std.dev: (352.00, 165.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1189) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (134, 1038, 1231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3425) [M::mem_pestat] mean and std.dev: (669.91, 588.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4522) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 234, 561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1317) [M::mem_pestat] mean and std.dev: (370.52, 298.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1695) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680810 reads in 224.962 CPU sec, 7.602 real sec sorting block 10, #reads = 1067291 mem = 536879086 peak = 957669376 [M::mem_process_seqs] Processed 680650 reads in 146.120 CPU sec, 4.981 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 277322, 12, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 294, 693) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1661) [M::mem_pestat] mean and std.dev: (345.92, 243.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2145) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (220, 332, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (348.49, 163.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (582, 2021, 6558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18510) [M::mem_pestat] mean and std.dev: (2624.17, 2527.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 24486) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 299, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981) [M::mem_pestat] mean and std.dev: (344.59, 189.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1231) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 11, #reads = 1067277 mem = 536876625 peak = 977842176 [M::process] read 681248 sequences (100000275 bp) in 2.541 CPU sec, 2.953 real sec... [M::process] read 681080 sequences (100000239 bp) in 2.066 CPU sec, 2.093 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 277192, 10, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 430, 699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1719) [M::mem_pestat] mean and std.dev: (416.18, 269.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2229) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (219, 332, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936) [M::mem_pestat] mean and std.dev: (348.49, 163.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1175) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (465, 2585, 4489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12537) [M::mem_pestat] mean and std.dev: (2599.40, 2512.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16561) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 284, 565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1259) [M::mem_pestat] mean and std.dev: (322.73, 159.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1606) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680930 reads in 223.917 CPU sec, 7.536 real sec [M::mem_process_seqs] Processed 681108 reads in 151.258 CPU sec, 5.071 real sec sorting block 12, #reads = 1067036 mem = 536878270 peak = 977842176 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 277183, 11, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 253, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046) [M::mem_pestat] mean and std.dev: (284.76, 140.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1328) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (219, 331, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (346.26, 161.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1159) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (59, 188, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1376) [M::mem_pestat] mean and std.dev: (228.20, 209.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1815) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 329, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (288.31, 133.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1191) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681010 sequences (100000048 bp) in 2.311 CPU sec, 2.552 real sec... [M::process] read 680986 sequences (100000099 bp) in 1.948 CPU sec, 1.958 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 277520, 14, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 221, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (255.30, 167.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1173) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (219, 332, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (347.53, 162.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1167) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (105, 247, 1070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3000) [M::mem_pestat] mean and std.dev: (513.64, 561.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3965) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 235, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961) [M::mem_pestat] mean and std.dev: (283.58, 217.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1221) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681248 reads in 225.967 CPU sec, 7.588 real sec [M::mem_process_seqs] Processed 681080 reads in 150.647 CPU sec, 5.084 real sec sorting block 13, #reads = 1067506 mem = 536883907 peak = 977842176 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 277016, 15, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 194, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (260.19, 156.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (220, 333, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (347.93, 162.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1164) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (701, 701, 4233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11297) [M::mem_pestat] mean and std.dev: (2213.87, 2167.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14829) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 317, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (295.42, 100.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680832 sequences (100000021 bp) in 3.096 CPU sec, 3.433 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 277617, 7, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (253, 348, 862) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2080) [M::mem_pestat] mean and std.dev: (433.86, 330.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (220, 333, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937) [M::mem_pestat] mean and std.dev: (348.74, 163.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1176) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 295, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (262.07, 95.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680800 sequences (100000300 bp) in 1.981 CPU sec, 2.020 real sec... [M::mem_process_seqs] Processed 681010 reads in 214.652 CPU sec, 7.191 real sec sorting block 14, #reads = 1067250 mem = 536882035 peak = 977842176 [M::mem_process_seqs] Processed 680986 reads in 137.714 CPU sec, 4.615 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 277531, 15, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 187, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1086) [M::mem_pestat] mean and std.dev: (252.89, 152.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (221, 335, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (349.92, 163.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1177) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 404, 867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2521) [M::mem_pestat] mean and std.dev: (493.20, 481.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3348) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (294, 334, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (74, 624) [M::mem_pestat] mean and std.dev: (327.81, 97.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680526 sequences (100000185 bp) in 2.502 CPU sec, 2.856 real sec... sorting block 15, #reads = 1066237 mem = 536882054 peak = 977842176 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 277508, 9, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 297, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (315.98, 162.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (222, 336, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936) [M::mem_pestat] mean and std.dev: (350.77, 163.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (245, 329, 685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1565) [M::mem_pestat] mean and std.dev: (412.59, 219.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2005) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679952 sequences (100000033 bp) in 1.946 CPU sec, 2.004 real sec... [M::mem_process_seqs] Processed 680832 reads in 210.487 CPU sec, 7.029 real sec [M::mem_process_seqs] Processed 680800 reads in 140.636 CPU sec, 4.695 real sec sorting block 16, #reads = 1065912 mem = 536877886 peak = 977842176 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 277663, 13, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 320, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (275.48, 112.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (225, 341, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945) [M::mem_pestat] mean and std.dev: (354.65, 165.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1185) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (716, 770, 2243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5297) [M::mem_pestat] mean and std.dev: (1238.08, 808.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6824) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (257, 370, 588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1250) [M::mem_pestat] mean and std.dev: (396.86, 189.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1581) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682246 sequences (100000160 bp) in 2.737 CPU sec, 3.041 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 278088, 9, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 262, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1090) [M::mem_pestat] mean and std.dev: (330.52, 242.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (227, 343, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950) [M::mem_pestat] mean and std.dev: (357.40, 166.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1191) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 266, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (319.23, 164.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684466 sequences (100000027 bp) in 2.076 CPU sec, 2.210 real sec... [M::mem_process_seqs] Processed 680526 reads in 205.563 CPU sec, 6.830 real sec [M::mem_process_seqs] Processed 679952 reads in 139.922 CPU sec, 4.660 real sec sorting block 17, #reads = 1064494 mem = 536886016 peak = 977842176 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 278023, 11, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 293, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1069) [M::mem_pestat] mean and std.dev: (348.49, 217.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1365) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (217, 331, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940) [M::mem_pestat] mean and std.dev: (347.59, 165.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1181) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (631, 1185, 1218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2392) [M::mem_pestat] mean and std.dev: (876.00, 431.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2979) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 243, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (234.09, 87.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683738 sequences (100000157 bp) in 2.287 CPU sec, 2.844 real sec... [M::process] read 683214 sequences (100000192 bp) in 1.841 CPU sec, 1.892 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 278634, 15, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 326, 1191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3137) [M::mem_pestat] mean and std.dev: (565.26, 487.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4110) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 320, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (339.84, 166.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1186) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (260, 260, 701) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1583) [M::mem_pestat] mean and std.dev: (431.79, 293.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2024) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (294, 345, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (64, 639) [M::mem_pestat] mean and std.dev: (321.38, 94.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682246 reads in 208.930 CPU sec, 6.962 real sec [M::mem_process_seqs] Processed 684466 reads in 140.203 CPU sec, 4.642 real sec sorting block 18, #reads = 1064436 mem = 536881793 peak = 977842176 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 277904, 6, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 292, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022) [M::mem_pestat] mean and std.dev: (286.38, 130.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1290) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 324, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (342.38, 166.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1192) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 261, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (236.45, 83.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683104 sequences (100000154 bp) in 2.915 CPU sec, 3.435 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 278102, 2, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 265, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (269.35, 145.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1086) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 327, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (343.88, 165.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1184) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (133, 212, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (237.00, 116.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682824 sequences (100000166 bp) in 1.979 CPU sec, 2.008 real sec... [M::mem_process_seqs] Processed 683738 reads in 201.848 CPU sec, 6.660 real sec sorting block 19, #reads = 1070041 mem = 536877648 peak = 977842176 [M::mem_process_seqs] Processed 683214 reads in 138.172 CPU sec, 4.515 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 278375, 14, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 289, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (251.81, 66.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 330, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954) [M::mem_pestat] mean and std.dev: (347.10, 166.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1201) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (138, 338, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1353) [M::mem_pestat] mean and std.dev: (295.00, 174.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1758) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (288, 440, 726) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1602) [M::mem_pestat] mean and std.dev: (481.83, 277.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2040) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 20, #reads = 1070472 mem = 536879336 peak = 1010790400 [M::process] read 682642 sequences (100000290 bp) in 2.526 CPU sec, 3.047 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 277905, 9, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 250, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (270.24, 111.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 332, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950) [M::mem_pestat] mean and std.dev: (347.87, 166.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1195) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 307, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (43, 503) [M::mem_pestat] mean and std.dev: (242.08, 73.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682362 sequences (100000019 bp) in 2.073 CPU sec, 2.111 real sec... [M::mem_process_seqs] Processed 683104 reads in 202.272 CPU sec, 6.704 real sec [M::mem_process_seqs] Processed 682824 reads in 137.625 CPU sec, 4.559 real sec sorting block 21, #reads = 1069794 mem = 536878535 peak = 1010790400 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 277964, 14, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 207, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833) [M::mem_pestat] mean and std.dev: (250.50, 118.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 334, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956) [M::mem_pestat] mean and std.dev: (349.02, 166.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1203) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (749, 927, 1993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4481) [M::mem_pestat] mean and std.dev: (1294.00, 947.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5725) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 212, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (263.03, 152.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682094 sequences (100000259 bp) in 2.791 CPU sec, 3.161 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 278102, 14, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 269, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (270.71, 129.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (217, 337, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961) [M::mem_pestat] mean and std.dev: (351.83, 167.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1209) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (482, 611, 884) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1688) [M::mem_pestat] mean and std.dev: (750.79, 547.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2939) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 365, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (359.59, 191.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1171) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681514 sequences (100000111 bp) in 2.137 CPU sec, 2.216 real sec... [M::mem_process_seqs] Processed 682642 reads in 204.622 CPU sec, 6.743 real sec [M::mem_process_seqs] Processed 682362 reads in 139.631 CPU sec, 4.621 real sec sorting block 22, #reads = 1069396 mem = 536881351 peak = 1010790400 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 277770, 7, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (238, 395, 631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1417) [M::mem_pestat] mean and std.dev: (426.06, 267.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1810) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (218, 339, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965) [M::mem_pestat] mean and std.dev: (353.35, 168.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1214) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 361, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (353.32, 114.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681432 sequences (100000027 bp) in 2.519 CPU sec, 2.941 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 277779, 3, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 316, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953) [M::mem_pestat] mean and std.dev: (329.96, 162.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1192) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (220, 341, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967) [M::mem_pestat] mean and std.dev: (354.96, 167.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1216) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (258, 320, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 639) [M::mem_pestat] mean and std.dev: (289.36, 88.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681472 sequences (100000252 bp) in 1.936 CPU sec, 1.981 real sec... [M::mem_process_seqs] Processed 682094 reads in 202.158 CPU sec, 6.611 real sec sorting block 23, #reads = 1069126 mem = 536880047 peak = 1010790400 [M::mem_process_seqs] Processed 681514 reads in 137.603 CPU sec, 4.466 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 277933, 16, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 346, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (326.29, 110.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (222, 341, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963) [M::mem_pestat] mean and std.dev: (355.47, 168.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1210) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (374, 540, 702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1358) [M::mem_pestat] mean and std.dev: (500.67, 333.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1835) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 280, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983) [M::mem_pestat] mean and std.dev: (333.64, 171.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1242) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681112 sequences (100000058 bp) in 2.718 CPU sec, 3.222 real sec... sorting block 24, #reads = 1068071 mem = 536878564 peak = 1043775488 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 278512, 10, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (234, 352, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (318.87, 152.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (221, 341, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962) [M::mem_pestat] mean and std.dev: (354.58, 167.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1209) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (93, 512, 1007) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2835) [M::mem_pestat] mean and std.dev: (658.00, 620.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3749) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 265, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (323.37, 174.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680838 sequences (100000188 bp) in 2.023 CPU sec, 2.035 real sec... [M::mem_process_seqs] Processed 681432 reads in 197.222 CPU sec, 6.540 real sec [M::mem_process_seqs] Processed 681472 reads in 133.132 CPU sec, 4.399 real sec sorting block 25, #reads = 1070176 mem = 536887321 peak = 1043775488 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 277186, 7, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 266, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986) [M::mem_pestat] mean and std.dev: (236.15, 119.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1272) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (223, 344, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970) [M::mem_pestat] mean and std.dev: (357.56, 168.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1219) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 253, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986) [M::mem_pestat] mean and std.dev: (251.86, 160.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1265) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680996 sequences (100000105 bp) in 2.458 CPU sec, 2.926 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 277957, 7, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 192, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992) [M::mem_pestat] mean and std.dev: (265.93, 165.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1268) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (224, 345, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983) [M::mem_pestat] mean and std.dev: (359.62, 169.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1236) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 325, 570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1248) [M::mem_pestat] mean and std.dev: (418.27, 278.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1587) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680572 sequences (100000193 bp) in 1.930 CPU sec, 1.973 real sec... [M::mem_process_seqs] Processed 681112 reads in 199.686 CPU sec, 6.535 real sec [M::mem_process_seqs] Processed 680838 reads in 136.293 CPU sec, 4.416 real sec sorting block 26, #reads = 1068803 mem = 536878124 peak = 1043775488 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 277558, 15, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 217, 542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1270) [M::mem_pestat] mean and std.dev: (243.87, 182.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (223, 345, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982) [M::mem_pestat] mean and std.dev: (359.69, 169.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1235) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (292, 658, 696) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1504) [M::mem_pestat] mean and std.dev: (495.71, 254.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1908) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 231, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (250.42, 106.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680212 sequences (100000130 bp) in 2.466 CPU sec, 2.874 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 277222, 12, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 417, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043) [M::mem_pestat] mean and std.dev: (384.62, 254.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (225, 346, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972) [M::mem_pestat] mean and std.dev: (359.10, 168.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1221) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (377, 1969, 2651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7199) [M::mem_pestat] mean and std.dev: (1718.17, 1365.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9473) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 344, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1224) [M::mem_pestat] mean and std.dev: (303.76, 190.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1572) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680720 sequences (100000163 bp) in 2.078 CPU sec, 2.220 real sec... [M::mem_process_seqs] Processed 680996 reads in 202.356 CPU sec, 6.620 real sec [M::mem_process_seqs] Processed 680572 reads in 138.371 CPU sec, 4.512 real sec sorting block 27, #reads = 1068289 mem = 536879171 peak = 1043775488 [M::process] read 680892 sequences (100000025 bp) in 2.496 CPU sec, 2.966 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 277305, 15, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 356, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1178) [M::mem_pestat] mean and std.dev: (371.75, 175.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (223, 341, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958) [M::mem_pestat] mean and std.dev: (355.00, 165.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1203) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (719, 900, 2264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5354) [M::mem_pestat] mean and std.dev: (1081.47, 819.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6899) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 361, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030) [M::mem_pestat] mean and std.dev: (351.49, 195.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1311) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680974 sequences (100000187 bp) in 1.981 CPU sec, 2.070 real sec... [M::mem_process_seqs] Processed 680720 reads in 121.764 CPU sec, 3.899 real sec sorting block 28, #reads = 1068173 mem = 536878378 peak = 1043775488 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 277164, 13, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 227, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1187) [M::mem_pestat] mean and std.dev: (295.36, 200.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (227, 348, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977) [M::mem_pestat] mean and std.dev: (362.30, 170.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1227) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (576, 933, 938) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1662) [M::mem_pestat] mean and std.dev: (730.92, 370.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2212) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 331, 704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1672) [M::mem_pestat] mean and std.dev: (464.61, 342.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2156) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 277566, 7, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 273, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (285.00, 144.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (222, 336, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (349.93, 162.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1170) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 324, 572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1258) [M::mem_pestat] mean and std.dev: (387.33, 258.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1601) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680212 reads in 320.878 CPU sec, 10.492 real sec [M::process] read 680572 sequences (100000272 bp) in 2.438 CPU sec, 2.551 real sec... sorting block 29, #reads = 1067534 mem = 536879563 peak = 1043775488 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 277001, 9, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 279, 583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1431) [M::mem_pestat] mean and std.dev: (366.12, 295.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1855) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (222, 338, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (351.31, 163.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1178) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 398, 885) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2207) [M::mem_pestat] mean and std.dev: (358.65, 242.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2868) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681006 sequences (100000123 bp) in 2.491 CPU sec, 2.543 real sec... [M::mem_process_seqs] Processed 680974 reads in 117.394 CPU sec, 3.774 real sec [M::mem_process_seqs] Processed 680892 reads in 327.546 CPU sec, 10.694 real sec sorting block 30, #reads = 1066172 mem = 536880783 peak = 1043775488 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 277023, 12, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 288, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (282.12, 115.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (222, 336, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (351.18, 163.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1178) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 16, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787) [M::mem_pestat] mean and std.dev: (124.25, 150.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 259, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (262.98, 112.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681018 sequences (100000075 bp) in 2.356 CPU sec, 2.723 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 277225, 18, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 283, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846) [M::mem_pestat] mean and std.dev: (318.44, 164.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (220, 334, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (348.89, 162.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (356, 363, 2015) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5333) [M::mem_pestat] mean and std.dev: (692.69, 644.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6992) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 242, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (303.90, 173.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1187) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680572 reads in 232.377 CPU sec, 7.596 real sec [M::process] read 680748 sequences (100000120 bp) in 2.279 CPU sec, 2.375 real sec... [M::mem_process_seqs] Processed 681006 reads in 142.404 CPU sec, 4.667 real sec sorting block 31, #reads = 1065502 mem = 536879424 peak = 1043775488 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 277309, 13, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 219, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (19, 409) [M::mem_pestat] mean and std.dev: (204.34, 64.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (220, 335, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940) [M::mem_pestat] mean and std.dev: (349.74, 163.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1180) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 56, 695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2027) [M::mem_pestat] mean and std.dev: (240.58, 269.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2693) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 357, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1111) [M::mem_pestat] mean and std.dev: (370.96, 242.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1413) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680922 sequences (100000261 bp) in 2.387 CPU sec, 2.935 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 277182, 7, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 349, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066) [M::mem_pestat] mean and std.dev: (295.05, 159.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (222, 336, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (351.19, 164.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1178) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 371, 557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1271) [M::mem_pestat] mean and std.dev: (318.57, 205.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1628) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681026 sequences (100000031 bp) in 2.182 CPU sec, 2.196 real sec... [M::mem_process_seqs] Processed 681018 reads in 209.124 CPU sec, 6.930 real sec [M::mem_process_seqs] Processed 680748 reads in 142.732 CPU sec, 4.695 real sec sorting block 32, #reads = 1066544 mem = 536877819 peak = 1043775488 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 277777, 3, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 361, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076) [M::mem_pestat] mean and std.dev: (405.67, 246.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1393) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (221, 336, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (351.20, 165.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1185) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (123, 192, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (218.44, 117.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1007) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681130 sequences (100000011 bp) in 2.796 CPU sec, 3.176 real sec... sorting block 33, #reads = 1066905 mem = 536880137 peak = 1043775488 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 277686, 2, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 263, 625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1479) [M::mem_pestat] mean and std.dev: (342.90, 275.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1906) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (222, 335, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936) [M::mem_pestat] mean and std.dev: (350.54, 163.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 239, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (253.28, 98.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681022 sequences (100000047 bp) in 2.111 CPU sec, 2.187 real sec... [M::mem_process_seqs] Processed 680922 reads in 208.624 CPU sec, 6.907 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 278177, 7, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 227, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (209.70, 82.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (220, 335, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937) [M::mem_pestat] mean and std.dev: (349.79, 163.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1176) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 262, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021) [M::mem_pestat] mean and std.dev: (334.72, 191.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1289) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680844 sequences (100000258 bp) in 2.181 CPU sec, 2.207 real sec... [M::mem_process_seqs] Processed 681130 reads in 124.308 CPU sec, 4.170 real sec sorting block 34, #reads = 1067582 mem = 536878524 peak = 1043775488 [M::mem_process_seqs] Processed 681026 reads in 314.092 CPU sec, 10.431 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 277654, 4, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 233, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841) [M::mem_pestat] mean and std.dev: (245.66, 120.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1067) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (222, 338, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948) [M::mem_pestat] mean and std.dev: (353.21, 165.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1190) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 251, 649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1513) [M::mem_pestat] mean and std.dev: (382.21, 348.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1945) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680506 sequences (100000173 bp) in 2.547 CPU sec, 3.013 real sec... sorting block 35, #reads = 1067037 mem = 536879840 peak = 1043775488 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 277769, 6, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 457, 1277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3361) [M::mem_pestat] mean and std.dev: (681.56, 603.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (223, 340, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958) [M::mem_pestat] mean and std.dev: (355.64, 167.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1203) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 219, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (243.86, 116.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681022 reads in 234.982 CPU sec, 7.854 real sec [M::process] read 682312 sequences (100000098 bp) in 2.206 CPU sec, 2.306 real sec... [M::mem_process_seqs] Processed 680844 reads in 143.517 CPU sec, 4.791 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 277448, 7, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 253, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034) [M::mem_pestat] mean and std.dev: (292.32, 173.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1336) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (225, 343, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963) [M::mem_pestat] mean and std.dev: (358.19, 168.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1209) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 263, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971) [M::mem_pestat] mean and std.dev: (270.44, 132.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1241) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 36, #reads = 1065519 mem = 536879142 peak = 1043775488 [M::process] read 685066 sequences (100000152 bp) in 2.843 CPU sec, 3.205 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 277641, 5, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (255, 309, 512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026) [M::mem_pestat] mean and std.dev: (265.26, 118.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1283) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 331, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950) [M::mem_pestat] mean and std.dev: (348.08, 166.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1195) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 213, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (261.39, 125.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684842 sequences (100000263 bp) in 1.693 CPU sec, 1.854 real sec... [M::mem_process_seqs] Processed 680506 reads in 215.645 CPU sec, 7.168 real sec [M::mem_process_seqs] Processed 682312 reads in 147.831 CPU sec, 4.905 real sec sorting block 37, #reads = 1064246 mem = 536879795 peak = 1043775488 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 279030, 13, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 236, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (244.15, 80.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 317, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (336.13, 164.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1176) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (279, 605, 1309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3369) [M::mem_pestat] mean and std.dev: (740.46, 742.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4399) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 233, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (268.79, 105.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684654 sequences (100000287 bp) in 2.792 CPU sec, 3.290 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 278822, 3, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 329, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1199) [M::mem_pestat] mean and std.dev: (310.23, 133.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 319, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (337.31, 164.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1184) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 286, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (258.78, 83.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685066 reads in 205.707 CPU sec, 6.917 real sec [M::process] read 684122 sequences (100000021 bp) in 2.201 CPU sec, 2.278 real sec... sorting block 38, #reads = 1067793 mem = 536880949 peak = 1043775488 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 278418, 17, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 152, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (197.79, 92.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 321, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (338.84, 164.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1182) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (93, 147, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960) [M::mem_pestat] mean and std.dev: (201.31, 209.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1249) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 343, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (295.84, 107.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683860 sequences (100000031 bp) in 2.368 CPU sec, 2.517 real sec... [M::mem_process_seqs] Processed 684654 reads in 114.387 CPU sec, 3.845 real sec [M::mem_process_seqs] Processed 684842 reads in 312.710 CPU sec, 10.431 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278701, 3, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 213, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (221.41, 90.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 323, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943) [M::mem_pestat] mean and std.dev: (340.82, 164.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1188) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (251, 368, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962) [M::mem_pestat] mean and std.dev: (363.80, 182.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1199) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 39, #reads = 1069523 mem = 536882491 peak = 1043775488 [M::process] read 683768 sequences (100000003 bp) in 2.695 CPU sec, 3.131 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 278335, 9, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 186, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (292.29, 204.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 324, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (341.99, 165.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1185) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 410, 1169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3087) [M::mem_pestat] mean and std.dev: (528.64, 579.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4046) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684122 reads in 223.580 CPU sec, 7.486 real sec [M::mem_process_seqs] Processed 683860 reads in 134.856 CPU sec, 4.461 real sec sorting block 40, #reads = 1071758 mem = 536880473 peak = 1043775488 [M::process] read 682984 sequences (100000253 bp) in 2.324 CPU sec, 2.498 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 278568, 14, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 389, 618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1230) [M::mem_pestat] mean and std.dev: (442.78, 282.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 327, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954) [M::mem_pestat] mean and std.dev: (344.08, 166.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1202) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (310, 431, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 673) [M::mem_pestat] mean and std.dev: (363.58, 108.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (257, 319, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (340.20, 153.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1005) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682720 sequences (100000054 bp) in 2.617 CPU sec, 2.778 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 277918, 13, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 235, 596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1430) [M::mem_pestat] mean and std.dev: (291.97, 158.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1847) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 330, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956) [M::mem_pestat] mean and std.dev: (346.69, 166.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1204) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 180, 1987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5913) [M::mem_pestat] mean and std.dev: (808.46, 1107.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7876) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 310, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948) [M::mem_pestat] mean and std.dev: (294.09, 108.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1191) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682998 sequences (100000086 bp) in 1.757 CPU sec, 1.765 real sec... [M::mem_process_seqs] Processed 683768 reads in 191.777 CPU sec, 6.101 real sec sorting block 41, #reads = 1070387 mem = 536877716 peak = 1043775488 [M::mem_process_seqs] Processed 682984 reads in 127.153 CPU sec, 4.030 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 278710, 11, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 256, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (280.43, 109.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 332, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955) [M::mem_pestat] mean and std.dev: (348.06, 166.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1202) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (718, 1209, 1209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2191) [M::mem_pestat] mean and std.dev: (976.64, 359.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2682) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 382, 633) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1557) [M::mem_pestat] mean and std.dev: (360.45, 203.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2019) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682246 sequences (100000267 bp) in 2.888 CPU sec, 3.118 real sec... sorting block 42, #reads = 1068658 mem = 536881073 peak = 1043775488 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 278615, 8, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (229, 241, 584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1294) [M::mem_pestat] mean and std.dev: (371.00, 258.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 331, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962) [M::mem_pestat] mean and std.dev: (347.56, 167.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1212) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 343, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1244) [M::mem_pestat] mean and std.dev: (362.73, 200.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1602) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681764 sequences (100000221 bp) in 1.980 CPU sec, 2.102 real sec... [M::mem_process_seqs] Processed 682720 reads in 199.158 CPU sec, 6.410 real sec [M::mem_process_seqs] Processed 682998 reads in 136.804 CPU sec, 4.421 real sec sorting block 43, #reads = 1070413 mem = 536879578 peak = 1043775488 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 278123, 4, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 306, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945) [M::mem_pestat] mean and std.dev: (344.51, 192.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1198) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (217, 338, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973) [M::mem_pestat] mean and std.dev: (353.13, 168.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1225) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 404, 672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1550) [M::mem_pestat] mean and std.dev: (456.98, 295.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1989) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681636 sequences (100000174 bp) in 2.559 CPU sec, 2.706 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 277270, 8, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 228, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (245.18, 157.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (219, 338, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963) [M::mem_pestat] mean and std.dev: (353.07, 167.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1211) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 250, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (241.46, 115.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681604 sequences (100000244 bp) in 1.850 CPU sec, 1.852 real sec... [M::mem_process_seqs] Processed 682246 reads in 180.354 CPU sec, 5.748 real sec [M::mem_process_seqs] Processed 681764 reads in 120.227 CPU sec, 3.805 real sec sorting block 44, #reads = 1068973 mem = 536880199 peak = 1043775488 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 277682, 7, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (256, 266, 555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1153) [M::mem_pestat] mean and std.dev: (358.65, 194.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (220, 342, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982) [M::mem_pestat] mean and std.dev: (356.86, 170.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1236) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 306, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (296.27, 119.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1065) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681358 sequences (100000069 bp) in 2.766 CPU sec, 2.908 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 277511, 11, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 298, 551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1231) [M::mem_pestat] mean and std.dev: (279.58, 178.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (219, 341, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987) [M::mem_pestat] mean and std.dev: (356.83, 170.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1243) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (119, 119, 3931) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11555) [M::mem_pestat] mean and std.dev: (1689.91, 2469.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15367) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 322, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (300.54, 130.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1017) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681524 sequences (100000174 bp) in 1.903 CPU sec, 1.904 real sec... [M::mem_process_seqs] Processed 681636 reads in 175.512 CPU sec, 5.563 real sec sorting block 45, #reads = 1068569 mem = 536878193 peak = 1043775488 [M::mem_process_seqs] Processed 681604 reads in 122.618 CPU sec, 3.888 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 277396, 18, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (193.75, 64.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (220, 342, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982) [M::mem_pestat] mean and std.dev: (357.26, 170.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1236) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (263, 1252, 1688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4538) [M::mem_pestat] mean and std.dev: (943.12, 647.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5963) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 258, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (296.75, 133.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681248 sequences (100000028 bp) in 3.061 CPU sec, 3.304 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 278317, 8, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 286, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (263.04, 120.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (221, 342, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974) [M::mem_pestat] mean and std.dev: (356.28, 168.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1225) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 457, 701) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1643) [M::mem_pestat] mean and std.dev: (409.57, 235.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2114) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682106 sequences (100000233 bp) in 1.922 CPU sec, 1.926 real sec... [M::mem_process_seqs] Processed 681358 reads in 191.490 CPU sec, 6.117 real sec sorting block 46, #reads = 1068206 mem = 536880267 peak = 1043775488 [M::mem_process_seqs] Processed 681524 reads in 124.893 CPU sec, 3.973 real sec sorting block 47, #reads = 1069023 mem = 536876464 peak = 1043775488 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 277200, 12, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 302, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (233.59, 87.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (222, 343, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978) [M::mem_pestat] mean and std.dev: (358.03, 169.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1230) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1365, 4576, 7040) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18390) [M::mem_pestat] mean and std.dev: (3909.58, 2617.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 24065) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 320, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (276.89, 124.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681734 sequences (100000152 bp) in 2.657 CPU sec, 2.811 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 277393, 14, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 265, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (252.95, 84.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 328, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (345.13, 163.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1175) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (145, 425, 2526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7288) [M::mem_pestat] mean and std.dev: (1247.21, 1670.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9669) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 403, 805) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2095) [M::mem_pestat] mean and std.dev: (329.96, 185.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2740) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682070 sequences (100000104 bp) in 1.727 CPU sec, 1.730 real sec... [M::mem_process_seqs] Processed 681248 reads in 184.126 CPU sec, 5.864 real sec [M::process] read 680782 sequences (100000287 bp) in 1.680 CPU sec, 1.687 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 277331, 9, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 288, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (312.92, 131.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 330, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (346.80, 164.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1180) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 210, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (260.87, 141.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681734 reads in 107.727 CPU sec, 3.657 real sec sorting block 48, #reads = 1067533 mem = 536879682 peak = 1043775488 [M::mem_process_seqs] Processed 682106 reads in 265.994 CPU sec, 8.699 real sec sorting block 49, #reads = 1067596 mem = 536882903 peak = 1049374720 [M::process] read 680622 sequences (100000224 bp) in 2.507 CPU sec, 2.649 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 277416, 5, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 262, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (274.17, 121.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (223, 341, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967) [M::mem_pestat] mean and std.dev: (356.46, 167.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1215) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 252, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1104) [M::mem_pestat] mean and std.dev: (276.94, 150.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1411) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680782 reads in 90.670 CPU sec, 2.850 real sec [M::process] read 680902 sequences (100000134 bp) in 1.912 CPU sec, 1.924 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 277854, 11, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 361, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1118) [M::mem_pestat] mean and std.dev: (326.55, 169.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 333, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951) [M::mem_pestat] mean and std.dev: (349.05, 166.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1196) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (261, 871, 3391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9651) [M::mem_pestat] mean and std.dev: (1644.09, 1462.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12781) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 269, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (235.26, 101.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 277271, 15, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 401, 1027) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2671) [M::mem_pestat] mean and std.dev: (577.04, 462.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (223, 342, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958) [M::mem_pestat] mean and std.dev: (356.02, 166.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1203) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (520, 978, 4433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12259) [M::mem_pestat] mean and std.dev: (1685.73, 1699.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16172) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 373, 570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1298) [M::mem_pestat] mean and std.dev: (415.31, 298.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1662) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682070 reads in 278.761 CPU sec, 8.847 real sec sorting block 50, #reads = 1067895 mem = 536884602 peak = 1049374720 [M::mem_process_seqs] Processed 680622 reads in 120.666 CPU sec, 3.806 real sec [M::process] read 680712 sequences (100000172 bp) in 2.338 CPU sec, 2.400 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 277508, 3, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 283, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (288.75, 169.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1183) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (223, 340, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958) [M::mem_pestat] mean and std.dev: (354.96, 166.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1203) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 253, 593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1445) [M::mem_pestat] mean and std.dev: (322.23, 270.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1871) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 51, #reads = 1067289 mem = 536879306 peak = 1049374720 [M::process] read 680862 sequences (100000014 bp) in 2.307 CPU sec, 2.432 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 277556, 10, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 249, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848) [M::mem_pestat] mean and std.dev: (255.00, 110.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (223, 342, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967) [M::mem_pestat] mean and std.dev: (357.15, 168.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1215) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (485, 1554, 4410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12260) [M::mem_pestat] mean and std.dev: (2607.10, 2873.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16185) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 240, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (242.40, 88.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 912) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680902 reads in 152.073 CPU sec, 4.827 real sec [M::process] read 680882 sequences (100000113 bp) in 1.750 CPU sec, 1.752 real sec... [M::mem_process_seqs] Processed 680712 reads in 99.514 CPU sec, 3.143 real sec sorting block 52, #reads = 1067150 mem = 536883473 peak = 1049374720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 277660, 10, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 274, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (277.00, 147.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 989) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (223, 341, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964) [M::mem_pestat] mean and std.dev: (356.24, 167.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1211) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (388, 834, 1788) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4588) [M::mem_pestat] mean and std.dev: (1012.70, 690.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5988) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (256, 288, 1280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3328) [M::mem_pestat] mean and std.dev: (501.44, 466.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4352) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680558 sequences (100000269 bp) in 2.797 CPU sec, 2.954 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 277247, 24, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 204, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (221.12, 84.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (223, 341, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961) [M::mem_pestat] mean and std.dev: (355.70, 167.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1207) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (89, 92, 1301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3725) [M::mem_pestat] mean and std.dev: (598.50, 831.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4937) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 235, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (246.88, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681262 sequences (100000261 bp) in 1.850 CPU sec, 1.846 real sec... [M::mem_process_seqs] Processed 680862 reads in 164.867 CPU sec, 5.221 real sec [M::mem_process_seqs] Processed 680882 reads in 112.096 CPU sec, 3.538 real sec sorting block 53, #reads = 1067524 mem = 536877523 peak = 1049374720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 277095, 9, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 269, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (278.16, 108.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (224, 340, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953) [M::mem_pestat] mean and std.dev: (355.45, 166.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1196) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 242, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (79, 424) [M::mem_pestat] mean and std.dev: (238.18, 69.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680136 sequences (100000265 bp) in 2.337 CPU sec, 2.460 real sec... [M::process] read 679954 sequences (100000248 bp) in 1.820 CPU sec, 1.822 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 277695, 7, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (248, 318, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986) [M::mem_pestat] mean and std.dev: (364.48, 188.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1232) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (219, 338, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966) [M::mem_pestat] mean and std.dev: (354.42, 168.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1215) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 323, 581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1385) [M::mem_pestat] mean and std.dev: (403.30, 295.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1787) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680558 reads in 171.444 CPU sec, 5.440 real sec sorting block 54, #reads = 1066749 mem = 536878460 peak = 1049374720 [M::process] read 683866 sequences (100000146 bp) in 1.609 CPU sec, 1.616 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 277485, 5, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 252, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969) [M::mem_pestat] mean and std.dev: (283.57, 172.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1239) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (226, 346, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976) [M::mem_pestat] mean and std.dev: (361.82, 171.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1226) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (255, 360, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846) [M::mem_pestat] mean and std.dev: (343.59, 113.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680136 reads in 98.903 CPU sec, 3.399 real sec [M::mem_process_seqs] Processed 681262 reads in 244.134 CPU sec, 8.026 real sec sorting block 55, #reads = 1065955 mem = 536879149 peak = 1049374720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 276726, 3, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 217, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012) [M::mem_pestat] mean and std.dev: (238.74, 115.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1301) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (228, 350, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984) [M::mem_pestat] mean and std.dev: (364.89, 172.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1236) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (136, 233, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991) [M::mem_pestat] mean and std.dev: (296.11, 193.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1276) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684554 sequences (100000218 bp) in 2.464 CPU sec, 2.627 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 277553, 8, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 226, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1223) [M::mem_pestat] mean and std.dev: (339.31, 271.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 324, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947) [M::mem_pestat] mean and std.dev: (342.81, 167.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1193) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 244, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (304.10, 210.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684112 sequences (100000083 bp) in 1.784 CPU sec, 1.793 real sec... [M::mem_process_seqs] Processed 679954 reads in 201.047 CPU sec, 6.380 real sec [M::mem_process_seqs] Processed 683866 reads in 129.283 CPU sec, 4.097 real sec sorting block 56, #reads = 1064772 mem = 536882276 peak = 1049374720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 277990, 10, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 304, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1181) [M::mem_pestat] mean and std.dev: (309.86, 224.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 320, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (338.55, 165.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1178) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (397, 406, 1019) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2263) [M::mem_pestat] mean and std.dev: (650.70, 350.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2885) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 200, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (235.15, 96.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683866 sequences (100000086 bp) in 2.782 CPU sec, 2.980 real sec... [M::process] read 683616 sequences (100000173 bp) in 1.855 CPU sec, 1.862 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 277934, 8, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 242, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (247.17, 145.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 321, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (339.74, 165.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1183) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (138, 201, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (215.79, 84.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684554 reads in 193.762 CPU sec, 6.167 real sec sorting block 57, #reads = 1066478 mem = 536884855 peak = 1056804864 [M::mem_process_seqs] Processed 684112 reads in 128.644 CPU sec, 4.095 real sec sorting block 58, #reads = 1070921 mem = 536879022 peak = 1115209728 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 278160, 11, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 315, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1143) [M::mem_pestat] mean and std.dev: (359.26, 191.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 322, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (339.65, 164.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1176) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (559, 559, 2128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5266) [M::mem_pestat] mean and std.dev: (1140.45, 953.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6835) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 235, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (10, 455) [M::mem_pestat] mean and std.dev: (241.78, 88.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683856 sequences (100000296 bp) in 2.538 CPU sec, 2.711 real sec... [M::process] read 683854 sequences (100000156 bp) in 1.890 CPU sec, 1.890 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 278478, 10, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 243, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985) [M::mem_pestat] mean and std.dev: (269.39, 143.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1256) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 323, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (340.36, 163.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1177) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (403, 540, 825) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1669) [M::mem_pestat] mean and std.dev: (531.22, 190.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2091) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (249, 434, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056) [M::mem_pestat] mean and std.dev: (378.17, 178.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1325) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683866 reads in 192.343 CPU sec, 6.129 real sec [M::mem_process_seqs] Processed 683616 reads in 128.192 CPU sec, 4.069 real sec sorting block 59, #reads = 1072001 mem = 536878952 peak = 1115209728 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278137, 19, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 333, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956) [M::mem_pestat] mean and std.dev: (289.97, 118.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1211) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 325, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950) [M::mem_pestat] mean and std.dev: (343.32, 166.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1197) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (630, 630, 3271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8553) [M::mem_pestat] mean and std.dev: (1609.79, 1638.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11194) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 236, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (257.57, 122.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683230 sequences (100000293 bp) in 2.852 CPU sec, 3.009 real sec... [M::process] read 683058 sequences (100000166 bp) in 1.878 CPU sec, 1.886 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 278446, 9, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 197, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010) [M::mem_pestat] mean and std.dev: (276.53, 163.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1294) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 325, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949) [M::mem_pestat] mean and std.dev: (342.73, 166.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1196) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 277, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1180) [M::mem_pestat] mean and std.dev: (326.43, 191.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1509) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683856 reads in 190.917 CPU sec, 6.075 real sec [M::mem_process_seqs] Processed 683854 reads in 127.674 CPU sec, 4.055 real sec sorting block 60, #reads = 1070456 mem = 536879259 peak = 1115209728 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 278256, 13, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 238, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (199.39, 72.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 329, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953) [M::mem_pestat] mean and std.dev: (345.93, 166.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1200) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (630, 820, 954) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1602) [M::mem_pestat] mean and std.dev: (712.31, 310.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1956) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 365, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1201) [M::mem_pestat] mean and std.dev: (379.18, 250.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1530) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683330 sequences (100000226 bp) in 2.609 CPU sec, 2.766 real sec... [M::process] read 682864 sequences (100000092 bp) in 1.888 CPU sec, 1.891 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 277731, 11, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 331, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (299.54, 116.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1151) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 329, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950) [M::mem_pestat] mean and std.dev: (344.95, 165.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1196) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (371, 948, 1602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4064) [M::mem_pestat] mean and std.dev: (861.80, 623.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5295) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (269, 390, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1097) [M::mem_pestat] mean and std.dev: (463.50, 302.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1674) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683230 reads in 192.000 CPU sec, 6.120 real sec sorting block 61, #reads = 1070089 mem = 536877467 peak = 1115209728 [M::mem_process_seqs] Processed 683058 reads in 129.268 CPU sec, 4.105 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 278071, 8, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 334, 575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1365) [M::mem_pestat] mean and std.dev: (359.21, 216.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 329, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955) [M::mem_pestat] mean and std.dev: (345.63, 166.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1203) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 354, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979) [M::mem_pestat] mean and std.dev: (326.15, 113.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1227) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 62, #reads = 1070430 mem = 536878333 peak = 1115209728 [M::process] read 682190 sequences (100000029 bp) in 2.892 CPU sec, 3.045 real sec... [M::process] read 681986 sequences (100000219 bp) in 1.876 CPU sec, 1.879 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 277789, 8, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 286, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022) [M::mem_pestat] mean and std.dev: (332.29, 207.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1298) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 332, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961) [M::mem_pestat] mean and std.dev: (348.83, 167.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1210) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 260, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (271.47, 114.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683330 reads in 193.450 CPU sec, 6.157 real sec [M::mem_process_seqs] Processed 682864 reads in 129.266 CPU sec, 4.107 real sec sorting block 63, #reads = 1069403 mem = 536877939 peak = 1115209728 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 277374, 8, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 242, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (276.62, 150.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1176) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 334, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966) [M::mem_pestat] mean and std.dev: (351.34, 168.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1216) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 463, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980) [M::mem_pestat] mean and std.dev: (374.81, 135.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1236) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681768 sequences (100000006 bp) in 2.600 CPU sec, 2.764 real sec... [M::process] read 681900 sequences (100000255 bp) in 1.871 CPU sec, 1.878 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 277201, 6, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 307, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (262.08, 105.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (217, 336, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970) [M::mem_pestat] mean and std.dev: (352.91, 168.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1221) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 265, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (268.60, 122.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682190 reads in 194.715 CPU sec, 6.205 real sec [M::mem_process_seqs] Processed 681986 reads in 131.278 CPU sec, 4.178 real sec sorting block 64, #reads = 1068777 mem = 536878345 peak = 1115209728 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 277591, 1, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 400, 634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1432) [M::mem_pestat] mean and std.dev: (391.81, 176.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1831) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (218, 339, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980) [M::mem_pestat] mean and std.dev: (355.24, 170.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1234) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 232, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (257.88, 132.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681640 sequences (100000141 bp) in 2.737 CPU sec, 2.868 real sec... [M::process] read 681418 sequences (100000166 bp) in 1.910 CPU sec, 1.902 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 277274, 8, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 419, 1621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4481) [M::mem_pestat] mean and std.dev: (746.90, 741.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5911) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (218, 339, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980) [M::mem_pestat] mean and std.dev: (355.30, 170.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1234) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 257, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (291.13, 144.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1179) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681768 reads in 197.392 CPU sec, 6.293 real sec [M::mem_process_seqs] Processed 681900 reads in 132.515 CPU sec, 4.214 real sec sorting block 65, #reads = 1069273 mem = 536881688 peak = 1115209728 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 277501, 17, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 288, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1212) [M::mem_pestat] mean and std.dev: (338.52, 221.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (220, 341, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979) [M::mem_pestat] mean and std.dev: (356.32, 170.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1232) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (375, 558, 1006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2268) [M::mem_pestat] mean and std.dev: (607.81, 332.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2899) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 240, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (257.80, 114.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681354 sequences (100000143 bp) in 2.601 CPU sec, 2.751 real sec... [M::process] read 681470 sequences (100000056 bp) in 1.901 CPU sec, 1.910 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 277231, 11, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 285, 512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1178) [M::mem_pestat] mean and std.dev: (307.64, 184.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (221, 341, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974) [M::mem_pestat] mean and std.dev: (355.93, 168.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1225) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (486, 509, 2563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6717) [M::mem_pestat] mean and std.dev: (1331.27, 1782.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8794) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 281, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (268.41, 138.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681640 reads in 197.592 CPU sec, 6.302 real sec sorting block 66, #reads = 1068921 mem = 536881022 peak = 1115209728 [M::mem_process_seqs] Processed 681418 reads in 133.610 CPU sec, 4.246 real sec sorting block 67, #reads = 1068739 mem = 536882677 peak = 1139093504 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 276967, 17, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 388, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1130) [M::mem_pestat] mean and std.dev: (402.64, 248.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1443) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (221, 341, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968) [M::mem_pestat] mean and std.dev: (355.43, 167.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1217) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (62, 469, 978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2810) [M::mem_pestat] mean and std.dev: (480.94, 352.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3726) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 246, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (236.18, 65.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680978 sequences (100000213 bp) in 2.954 CPU sec, 3.096 real sec... [M::process] read 680752 sequences (100000238 bp) in 1.869 CPU sec, 1.870 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 277283, 11, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 347, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956) [M::mem_pestat] mean and std.dev: (347.39, 167.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1207) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (221, 340, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959) [M::mem_pestat] mean and std.dev: (354.06, 166.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1205) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1244, 1244, 1889) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3179) [M::mem_pestat] mean and std.dev: (1160.09, 634.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3824) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 307, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (280.74, 95.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681354 reads in 197.192 CPU sec, 6.283 real sec [M::mem_process_seqs] Processed 681470 reads in 129.853 CPU sec, 4.133 real sec sorting block 68, #reads = 1067031 mem = 536879245 peak = 1139093504 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 277403, 4, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 227, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (251.37, 85.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (223, 342, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958) [M::mem_pestat] mean and std.dev: (355.33, 166.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1203) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 242, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (296.93, 163.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680718 sequences (100000285 bp) in 2.413 CPU sec, 2.529 real sec... [M::process] read 680208 sequences (100000004 bp) in 1.888 CPU sec, 1.900 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 277155, 8, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 207, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1105) [M::mem_pestat] mean and std.dev: (291.23, 173.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (225, 343, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963) [M::mem_pestat] mean and std.dev: (356.96, 166.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1209) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 258, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940) [M::mem_pestat] mean and std.dev: (307.93, 137.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1183) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680978 reads in 195.700 CPU sec, 6.242 real sec [M::mem_process_seqs] Processed 680752 reads in 131.379 CPU sec, 4.184 real sec sorting block 69, #reads = 1067720 mem = 536882005 peak = 1139093504 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 277408, 5, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (252, 306, 595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1281) [M::mem_pestat] mean and std.dev: (458.57, 347.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1849) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (225, 343, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960) [M::mem_pestat] mean and std.dev: (356.18, 165.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1205) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (274, 358, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (78, 568) [M::mem_pestat] mean and std.dev: (310.61, 89.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680252 sequences (100000226 bp) in 2.825 CPU sec, 2.960 real sec... [M::process] read 680248 sequences (100000081 bp) in 1.917 CPU sec, 1.922 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 277045, 12, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 257, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1111) [M::mem_pestat] mean and std.dev: (284.74, 163.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (226, 344, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961) [M::mem_pestat] mean and std.dev: (357.85, 166.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1206) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 937, 3029) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9035) [M::mem_pestat] mean and std.dev: (1253.58, 1247.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12038) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 326, 602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1342) [M::mem_pestat] mean and std.dev: (344.78, 204.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1712) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680718 reads in 194.934 CPU sec, 6.203 real sec sorting block 70, #reads = 1067993 mem = 536882353 peak = 1139093504 [M::mem_process_seqs] Processed 680208 reads in 131.704 CPU sec, 4.186 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 277026, 6, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 202, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054) [M::mem_pestat] mean and std.dev: (274.77, 173.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (226, 343, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952) [M::mem_pestat] mean and std.dev: (356.35, 165.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1194) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (130, 194, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (184.89, 93.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 71, #reads = 1064933 mem = 536879218 peak = 1139093504 [M::process] read 680276 sequences (100000109 bp) in 2.591 CPU sec, 2.743 real sec... [M::process] read 680532 sequences (100000127 bp) in 1.732 CPU sec, 1.766 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 277285, 5, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 271, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (275.42, 131.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (227, 344, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959) [M::mem_pestat] mean and std.dev: (358.29, 166.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1203) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 303, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (297.61, 88.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680252 reads in 193.779 CPU sec, 6.193 real sec [M::mem_process_seqs] Processed 680248 reads in 131.408 CPU sec, 4.205 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 277315, 9, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 202, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (190.16, 81.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (226, 343, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955) [M::mem_pestat] mean and std.dev: (356.67, 165.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1198) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 272, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (262.19, 87.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 72, #reads = 1065851 mem = 536879710 peak = 1139093504 [M::process] read 680414 sequences (100000195 bp) in 2.895 CPU sec, 3.219 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 277050, 2, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 239, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (261.56, 142.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (225, 341, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945) [M::mem_pestat] mean and std.dev: (354.75, 164.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1185) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 331, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (316.46, 159.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680436 sequences (100000186 bp) in 2.020 CPU sec, 2.142 real sec... [M::mem_process_seqs] Processed 680276 reads in 202.562 CPU sec, 6.540 real sec [M::mem_process_seqs] Processed 680532 reads in 138.275 CPU sec, 4.467 real sec sorting block 73, #reads = 1065952 mem = 536884076 peak = 1139093504 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 277099, 7, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 290, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (264.27, 127.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (224, 339, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (353.60, 164.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1184) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 293, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1189) [M::mem_pestat] mean and std.dev: (339.17, 201.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1512) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680188 sequences (100000278 bp) in 2.508 CPU sec, 2.921 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 276644, 12, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 206, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (194.64, 71.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (224, 340, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (354.32, 164.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1184) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (753, 837, 1507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3015) [M::mem_pestat] mean and std.dev: (959.25, 581.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3769) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (267, 339, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (333.43, 142.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686580 sequences (100000026 bp) in 2.132 CPU sec, 2.149 real sec... [M::mem_process_seqs] Processed 680414 reads in 200.981 CPU sec, 6.508 real sec [M::mem_process_seqs] Processed 680436 reads in 139.712 CPU sec, 4.513 real sec sorting block 74, #reads = 1066339 mem = 536880307 peak = 1139093504 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 277121, 15, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 212, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (240.21, 131.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (226, 343, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955) [M::mem_pestat] mean and std.dev: (357.25, 166.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1198) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (613, 1139, 2318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5728) [M::mem_pestat] mean and std.dev: (1352.87, 1155.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7433) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (256, 431, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979) [M::mem_pestat] mean and std.dev: (380.33, 158.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1220) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687508 sequences (100000165 bp) in 2.967 CPU sec, 3.141 real sec... [M::process] read 686892 sequences (100000182 bp) in 2.067 CPU sec, 2.077 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 279410, 8, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (378, 963, 1969) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5151) [M::mem_pestat] mean and std.dev: (1002.22, 727.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 305, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (327.54, 162.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 337, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967) [M::mem_pestat] mean and std.dev: (309.53, 136.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1223) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680188 reads in 215.606 CPU sec, 6.917 real sec sorting block 75, #reads = 1064204 mem = 536881328 peak = 1139093504 [M::mem_process_seqs] Processed 686580 reads in 154.182 CPU sec, 4.917 real sec sorting block 76, #reads = 1062798 mem = 536884403 peak = 1171980288 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 280204, 6, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 412, 692) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1650) [M::mem_pestat] mean and std.dev: (427.35, 343.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2129) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 303, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (327.85, 165.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1182) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (275, 331, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (348.75, 143.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686856 sequences (100000068 bp) in 2.667 CPU sec, 2.861 real sec... [M::process] read 685068 sequences (100000238 bp) in 2.029 CPU sec, 2.038 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 280321, 8, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 437, 602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1332) [M::mem_pestat] mean and std.dev: (436.48, 264.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1697) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 308, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (331.49, 166.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1196) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 232, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052) [M::mem_pestat] mean and std.dev: (327.72, 192.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1331) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687508 reads in 216.267 CPU sec, 6.917 real sec [M::mem_process_seqs] Processed 686892 reads in 144.687 CPU sec, 4.611 real sec sorting block 77, #reads = 1066781 mem = 536877403 peak = 1171980288 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 280666, 11, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 264, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (265.00, 103.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 308, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (330.55, 164.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1185) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (450, 1060, 1060) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2280) [M::mem_pestat] mean and std.dev: (748.50, 390.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2890) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 306, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (293.17, 97.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684264 sequences (100000028 bp) in 2.805 CPU sec, 3.066 real sec... [M::process] read 683778 sequences (100000072 bp) in 1.990 CPU sec, 1.998 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 279900, 9, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 278, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (255.53, 83.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 320, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954) [M::mem_pestat] mean and std.dev: (339.16, 166.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1204) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 274, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (270.86, 121.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1123) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686856 reads in 213.558 CPU sec, 6.834 real sec [M::mem_process_seqs] Processed 685068 reads in 139.394 CPU sec, 4.453 real sec sorting block 78, #reads = 1067839 mem = 536877284 peak = 1171980288 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 279253, 3, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 174, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (230.38, 106.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 326, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969) [M::mem_pestat] mean and std.dev: (344.42, 168.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1223) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 265, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (280.26, 107.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1022) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683094 sequences (100000133 bp) in 2.590 CPU sec, 2.767 real sec... [M::process] read 682970 sequences (100000104 bp) in 2.027 CPU sec, 2.033 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 279525, 8, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 267, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976) [M::mem_pestat] mean and std.dev: (329.46, 232.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1261) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 329, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971) [M::mem_pestat] mean and std.dev: (346.18, 167.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1225) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 325, 1025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2637) [M::mem_pestat] mean and std.dev: (630.67, 667.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3443) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684264 reads in 205.988 CPU sec, 6.593 real sec sorting block 79, #reads = 1068437 mem = 536882792 peak = 1171980288 [M::mem_process_seqs] Processed 683778 reads in 138.702 CPU sec, 4.423 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 279381, 5, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 206, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (234.00, 111.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 333, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980) [M::mem_pestat] mean and std.dev: (349.17, 168.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1236) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 308, 751) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1831) [M::mem_pestat] mean and std.dev: (362.50, 274.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2371) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 80, #reads = 1066860 mem = 536878716 peak = 1171980288 [M::process] read 682694 sequences (100000250 bp) in 2.782 CPU sec, 2.951 real sec... [M::process] read 682992 sequences (100000024 bp) in 2.014 CPU sec, 2.019 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 278462, 13, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 196, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1086) [M::mem_pestat] mean and std.dev: (295.03, 202.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 336, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987) [M::mem_pestat] mean and std.dev: (351.89, 169.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1245) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (7, 26, 790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2356) [M::mem_pestat] mean and std.dev: (240.33, 377.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3139) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 238, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (252.29, 113.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1093) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683094 reads in 206.050 CPU sec, 6.616 real sec [M::mem_process_seqs] Processed 682970 reads in 137.331 CPU sec, 4.401 real sec sorting block 81, #reads = 1068585 mem = 536878330 peak = 1171980288 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 278587, 1, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 307, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (308.55, 107.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 338, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986) [M::mem_pestat] mean and std.dev: (353.01, 169.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1243) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 323, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (315.22, 166.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682340 sequences (100000184 bp) in 2.626 CPU sec, 2.876 real sec... [M::process] read 682310 sequences (100000168 bp) in 1.965 CPU sec, 1.977 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 279779, 2, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 269, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (340.15, 195.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 337, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979) [M::mem_pestat] mean and std.dev: (351.54, 168.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1234) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 221, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (247.86, 91.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682694 reads in 206.798 CPU sec, 6.638 real sec [M::mem_process_seqs] Processed 682992 reads in 140.231 CPU sec, 4.486 real sec sorting block 82, #reads = 1067581 mem = 536878292 peak = 1171980288 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 279097, 3, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 325, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (297.76, 142.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1159) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 341, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999) [M::mem_pestat] mean and std.dev: (356.30, 171.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1260) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 331, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (341.05, 223.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1234) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682546 sequences (100000093 bp) in 3.031 CPU sec, 3.260 real sec... [M::process] read 681996 sequences (100000191 bp) in 2.080 CPU sec, 2.086 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 278178, 12, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 260, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1185) [M::mem_pestat] mean and std.dev: (281.20, 204.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (217, 341, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994) [M::mem_pestat] mean and std.dev: (356.76, 171.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1253) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (8, 1673, 1700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5084) [M::mem_pestat] mean and std.dev: (877.50, 848.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6776) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 314, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1057) [M::mem_pestat] mean and std.dev: (302.52, 157.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1341) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682340 reads in 210.876 CPU sec, 6.745 real sec [M::mem_process_seqs] Processed 682310 reads in 140.808 CPU sec, 4.498 real sec sorting block 83, #reads = 1065997 mem = 536878536 peak = 1171980288 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 278121, 5, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 217, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (263.00, 153.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 340, 478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004) [M::mem_pestat] mean and std.dev: (356.46, 172.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1267) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (311, 356, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (320.15, 100.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681858 sequences (100000164 bp) in 2.771 CPU sec, 2.968 real sec... [M::process] read 681828 sequences (100000005 bp) in 2.012 CPU sec, 2.036 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 277986, 7, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 217, 607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1513) [M::mem_pestat] mean and std.dev: (373.88, 280.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1966) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (217, 338, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979) [M::mem_pestat] mean and std.dev: (353.86, 168.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1233) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 347, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1112) [M::mem_pestat] mean and std.dev: (329.10, 184.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1414) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682546 reads in 217.071 CPU sec, 6.945 real sec sorting block 84, #reads = 1065640 mem = 536876884 peak = 1171980288 [M::mem_process_seqs] Processed 681996 reads in 147.549 CPU sec, 4.709 real sec sorting block 85, #reads = 1063870 mem = 536880170 peak = 1180286976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 277744, 7, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 294, 2267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6431) [M::mem_pestat] mean and std.dev: (822.38, 1254.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (217, 337, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979) [M::mem_pestat] mean and std.dev: (353.39, 168.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1233) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 275, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (288.82, 145.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1173) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681334 sequences (100000093 bp) in 3.116 CPU sec, 3.381 real sec... [M::process] read 681632 sequences (100000244 bp) in 2.019 CPU sec, 2.020 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 277381, 12, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 378, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (293.24, 115.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (217, 338, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982) [M::mem_pestat] mean and std.dev: (353.61, 168.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1237) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (367, 934, 2951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8119) [M::mem_pestat] mean and std.dev: (1344.75, 1225.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10703) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 246, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1107) [M::mem_pestat] mean and std.dev: (339.67, 236.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1416) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681858 reads in 220.351 CPU sec, 7.071 real sec [M::mem_process_seqs] Processed 681828 reads in 148.366 CPU sec, 4.772 real sec sorting block 86, #reads = 1062668 mem = 536879943 peak = 1180286976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 277711, 11, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 199, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (225.71, 124.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (219, 340, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981) [M::mem_pestat] mean and std.dev: (355.47, 168.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1235) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (906, 1334, 1538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2802) [M::mem_pestat] mean and std.dev: (935.78, 513.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3434) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 295, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025) [M::mem_pestat] mean and std.dev: (296.53, 130.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1302) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681786 sequences (100000143 bp) in 2.850 CPU sec, 3.047 real sec... [M::process] read 681978 sequences (100000089 bp) in 1.957 CPU sec, 1.966 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 277331, 7, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 302, 650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1528) [M::mem_pestat] mean and std.dev: (306.19, 165.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1967) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (219, 338, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972) [M::mem_pestat] mean and std.dev: (353.68, 167.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1223) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 240, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (298.64, 161.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681334 reads in 213.199 CPU sec, 6.828 real sec [M::mem_process_seqs] Processed 681632 reads in 139.844 CPU sec, 4.455 real sec sorting block 87, #reads = 1063410 mem = 536880256 peak = 1180286976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 277780, 9, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 286, 618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1552) [M::mem_pestat] mean and std.dev: (298.16, 223.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2019) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (218, 338, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983) [M::mem_pestat] mean and std.dev: (354.33, 168.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1238) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 326, 617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1403) [M::mem_pestat] mean and std.dev: (349.28, 217.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1796) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682050 sequences (100000180 bp) in 3.072 CPU sec, 3.184 real sec... [M::process] read 681972 sequences (100000148 bp) in 2.070 CPU sec, 2.073 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 278408, 9, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 272, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (276.82, 102.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (217, 336, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970) [M::mem_pestat] mean and std.dev: (351.68, 167.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1221) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 346, 546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1170) [M::mem_pestat] mean and std.dev: (330.47, 134.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1482) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681786 reads in 199.905 CPU sec, 6.349 real sec sorting block 88, #reads = 1064459 mem = 536880056 peak = 1180286976 [M::mem_process_seqs] Processed 681978 reads in 133.186 CPU sec, 4.237 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 277229, 6, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 177, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (239.15, 124.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 334, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966) [M::mem_pestat] mean and std.dev: (350.15, 166.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1216) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 333, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1073) [M::mem_pestat] mean and std.dev: (334.49, 192.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1358) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 89, #reads = 1064303 mem = 536880680 peak = 1180286976 [M::process] read 681896 sequences (100000101 bp) in 2.693 CPU sec, 2.873 real sec... [M::process] read 681778 sequences (100000025 bp) in 2.034 CPU sec, 2.041 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 278521, 13, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 270, 653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1629) [M::mem_pestat] mean and std.dev: (353.38, 244.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2117) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 333, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971) [M::mem_pestat] mean and std.dev: (350.23, 167.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1223) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (356, 415, 1391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3461) [M::mem_pestat] mean and std.dev: (801.08, 570.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4496) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 308, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (311.40, 134.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682050 reads in 213.835 CPU sec, 6.829 real sec [M::mem_process_seqs] Processed 681972 reads in 145.928 CPU sec, 4.657 real sec sorting block 90, #reads = 1062473 mem = 536877686 peak = 1180286976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278351, 4, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 242, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (262.38, 162.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (217, 336, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973) [M::mem_pestat] mean and std.dev: (352.23, 167.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1225) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 327, 588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1394) [M::mem_pestat] mean and std.dev: (423.25, 347.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1814) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681194 sequences (100000048 bp) in 3.133 CPU sec, 3.260 real sec... [M::process] read 681436 sequences (100000066 bp) in 1.969 CPU sec, 1.978 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 277206, 21, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 237, 589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1395) [M::mem_pestat] mean and std.dev: (290.08, 185.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (218, 335, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965) [M::mem_pestat] mean and std.dev: (352.17, 167.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1214) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (298, 884, 2735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7609) [M::mem_pestat] mean and std.dev: (1236.48, 1058.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10046) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 245, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (259.83, 113.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681896 reads in 214.577 CPU sec, 6.849 real sec [M::mem_process_seqs] Processed 681778 reads in 142.338 CPU sec, 4.522 real sec sorting block 91, #reads = 1063689 mem = 536878263 peak = 1180286976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 277593, 4, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 270, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1065) [M::mem_pestat] mean and std.dev: (353.18, 218.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (219, 336, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966) [M::mem_pestat] mean and std.dev: (352.73, 166.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1215) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 251, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1319) [M::mem_pestat] mean and std.dev: (321.48, 182.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1702) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681954 sequences (100000115 bp) in 2.886 CPU sec, 2.968 real sec... [M::process] read 689520 sequences (100000097 bp) in 1.918 CPU sec, 1.926 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 277883, 5, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 441, 572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1254) [M::mem_pestat] mean and std.dev: (367.42, 193.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (218, 337, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968) [M::mem_pestat] mean and std.dev: (353.33, 168.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1218) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (134, 231, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (279.86, 192.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681194 reads in 199.226 CPU sec, 6.333 real sec sorting block 92, #reads = 1061983 mem = 536878226 peak = 1180286976 [M::mem_process_seqs] Processed 681436 reads in 134.083 CPU sec, 4.260 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 278407, 14, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 267, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (303.16, 164.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1073) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (217, 337, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973) [M::mem_pestat] mean and std.dev: (353.34, 169.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1225) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (396, 1541, 3142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8634) [M::mem_pestat] mean and std.dev: (1544.00, 1360.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11380) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (260, 278, 512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016) [M::mem_pestat] mean and std.dev: (399.33, 209.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1268) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688950 sequences (100000074 bp) in 3.025 CPU sec, 3.110 real sec... [M::process] read 688540 sequences (100000300 bp) in 1.950 CPU sec, 1.950 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 281502, 15, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 164, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (158.64, 48.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 399) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 292, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (314.22, 155.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (298, 468, 1021) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2467) [M::mem_pestat] mean and std.dev: (623.71, 525.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3190) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (267, 444, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (370.47, 142.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681954 reads in 213.946 CPU sec, 6.802 real sec sorting block 93, #reads = 1062097 mem = 536878397 peak = 1180286976 [M::mem_process_seqs] Processed 689520 reads in 148.757 CPU sec, 4.734 real sec sorting block 94, #reads = 1060585 mem = 536879642 peak = 1180286976 [M::process] read 688234 sequences (100000282 bp) in 2.558 CPU sec, 2.649 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 281416, 6, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 254, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (261.58, 103.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 293, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (315.75, 156.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 315, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005) [M::mem_pestat] mean and std.dev: (325.23, 203.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1272) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687024 sequences (100000092 bp) in 2.010 CPU sec, 2.012 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 280704, 11, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 262, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (284.54, 140.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 298, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (318.14, 155.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (442, 609, 952) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1972) [M::mem_pestat] mean and std.dev: (521.89, 286.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2482) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 262, 572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1354) [M::mem_pestat] mean and std.dev: (320.23, 193.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1745) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 281664, 15, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 331, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1144) [M::mem_pestat] mean and std.dev: (332.57, 180.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 299, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (319.79, 157.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (328, 667, 5449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15691) [M::mem_pestat] mean and std.dev: (2161.67, 2490.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20812) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 365, 794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1902) [M::mem_pestat] mean and std.dev: (443.55, 267.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2456) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688950 reads in 311.224 CPU sec, 9.949 real sec [M::mem_process_seqs] Processed 688234 reads in 238.247 CPU sec, 7.639 real sec sorting block 95, #reads = 1061939 mem = 536878602 peak = 1180286976 [M::mem_process_seqs] Processed 688540 reads in 264.408 CPU sec, 8.487 real sec [M::process] read 686214 sequences (100000137 bp) in 2.876 CPU sec, 3.004 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 280988, 15, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 211, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (205.53, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 305, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (323.39, 156.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (667, 2633, 3875) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10291) [M::mem_pestat] mean and std.dev: (2694.40, 2068.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13499) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 285, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (295.00, 192.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1093) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 96, #reads = 1061573 mem = 536880494 peak = 1180286976 [M::process] read 685834 sequences (100000023 bp) in 2.631 CPU sec, 2.804 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 280238, 8, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 296, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1286) [M::mem_pestat] mean and std.dev: (322.59, 204.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 309, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (327.14, 157.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1158) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 309, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (318.13, 134.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 908) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685296 sequences (100000226 bp) in 1.870 CPU sec, 1.872 real sec... [M::mem_process_seqs] Processed 687024 reads in 228.786 CPU sec, 7.301 real sec [M::mem_process_seqs] Processed 686214 reads in 154.557 CPU sec, 5.031 real sec sorting block 97, #reads = 1060873 mem = 536879114 peak = 1180286976 [M::process] read 684442 sequences (100000011 bp) in 2.607 CPU sec, 2.773 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 280640, 0, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 267, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (263.44, 122.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 314, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (331.84, 160.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1180) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 339, 571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1329) [M::mem_pestat] mean and std.dev: (351.00, 189.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1708) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684048 sequences (100000118 bp) in 1.977 CPU sec, 1.978 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 279852, 5, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 295, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (22, 532) [M::mem_pestat] mean and std.dev: (270.33, 76.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 321, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (337.30, 160.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1182) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 339, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060) [M::mem_pestat] mean and std.dev: (400.59, 263.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1454) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 280376, 3, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 239, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (284.28, 178.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 997) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 318, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (334.55, 160.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1183) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (252, 467, 935) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2301) [M::mem_pestat] mean and std.dev: (626.11, 526.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2984) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685834 reads in 296.598 CPU sec, 9.595 real sec sorting block 98, #reads = 1062242 mem = 536883526 peak = 1180286976 [M::mem_process_seqs] Processed 684442 reads in 223.301 CPU sec, 7.155 real sec [M::mem_process_seqs] Processed 685296 reads in 247.321 CPU sec, 7.940 real sec sorting block 99, #reads = 1063192 mem = 536880658 peak = 1180286976 [M::process] read 683824 sequences (100000177 bp) in 2.576 CPU sec, 2.757 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 279544, 7, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 233, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1228) [M::mem_pestat] mean and std.dev: (323.00, 284.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 324, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940) [M::mem_pestat] mean and std.dev: (339.34, 161.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1184) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (238, 351, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (340.76, 154.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683654 sequences (100000270 bp) in 1.707 CPU sec, 1.760 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 279784, 8, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 279, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (316.35, 174.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1183) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 326, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949) [M::mem_pestat] mean and std.dev: (341.49, 163.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1196) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 261, 642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1530) [M::mem_pestat] mean and std.dev: (392.04, 304.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1974) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684048 reads in 164.576 CPU sec, 5.243 real sec [M::process] read 683402 sequences (100000118 bp) in 1.471 CPU sec, 1.482 real sec... [M::mem_process_seqs] Processed 683824 reads in 106.034 CPU sec, 3.527 real sec sorting block 100, #reads = 1063441 mem = 536882613 peak = 1180286976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 279856, 4, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 248, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (235.74, 90.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 329, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960) [M::mem_pestat] mean and std.dev: (344.66, 164.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1210) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 253, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (237.15, 89.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683244 sequences (100000198 bp) in 2.394 CPU sec, 2.543 real sec... [M::process] read 683066 sequences (100000067 bp) in 1.880 CPU sec, 1.883 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 279315, 18, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 265, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987) [M::mem_pestat] mean and std.dev: (293.26, 176.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1259) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 329, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966) [M::mem_pestat] mean and std.dev: (345.50, 166.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1218) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (144, 292, 749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1959) [M::mem_pestat] mean and std.dev: (444.82, 406.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2564) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 265, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024) [M::mem_pestat] mean and std.dev: (305.66, 139.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1302) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683654 reads in 177.852 CPU sec, 5.825 real sec sorting block 101, #reads = 1064255 mem = 536879216 peak = 1180286976 [M::mem_process_seqs] Processed 683402 reads in 120.580 CPU sec, 3.905 real sec sorting block 102, #reads = 1064413 mem = 536878453 peak = 1180286976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 279164, 8, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (242, 426, 966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2414) [M::mem_pestat] mean and std.dev: (588.72, 599.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3138) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 330, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970) [M::mem_pestat] mean and std.dev: (346.93, 166.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1223) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 258, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1118) [M::mem_pestat] mean and std.dev: (328.67, 206.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1427) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682646 sequences (100000285 bp) in 2.655 CPU sec, 2.797 real sec... [M::process] read 682186 sequences (100000256 bp) in 1.968 CPU sec, 1.975 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 279095, 15, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 327, 1030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2658) [M::mem_pestat] mean and std.dev: (697.79, 743.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 326, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948) [M::mem_pestat] mean and std.dev: (342.81, 163.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1194) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (296, 356, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (42, 677) [M::mem_pestat] mean and std.dev: (325.08, 97.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 289, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (316.64, 171.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1164) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683244 reads in 202.758 CPU sec, 6.522 real sec [M::mem_process_seqs] Processed 683066 reads in 128.868 CPU sec, 4.172 real sec sorting block 103, #reads = 1065243 mem = 536879946 peak = 1180286976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 278313, 4, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 319, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1174) [M::mem_pestat] mean and std.dev: (346.32, 211.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 333, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967) [M::mem_pestat] mean and std.dev: (349.30, 166.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1218) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 273, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (292.47, 163.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1089) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682242 sequences (100000194 bp) in 2.519 CPU sec, 2.673 real sec... [M::process] read 681666 sequences (100000059 bp) in 1.410 CPU sec, 1.410 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278270, 20, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 394, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1269) [M::mem_pestat] mean and std.dev: (334.58, 194.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 335, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974) [M::mem_pestat] mean and std.dev: (351.36, 168.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1227) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (100, 391, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1087) [M::mem_pestat] mean and std.dev: (267.06, 218.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1416) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 303, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (285.68, 121.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1016) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682646 reads in 177.232 CPU sec, 5.722 real sec [M::mem_process_seqs] Processed 682186 reads in 107.196 CPU sec, 3.413 real sec sorting block 104, #reads = 1062982 mem = 536886550 peak = 1180286976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 277548, 10, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 300, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008) [M::mem_pestat] mean and std.dev: (290.89, 164.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1293) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 335, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980) [M::mem_pestat] mean and std.dev: (352.01, 168.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1235) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (199, 486, 814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2044) [M::mem_pestat] mean and std.dev: (508.80, 301.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2659) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (288, 361, 583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1173) [M::mem_pestat] mean and std.dev: (413.58, 226.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1468) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681912 sequences (100000141 bp) in 2.110 CPU sec, 2.217 real sec... [M::process] read 681798 sequences (100000285 bp) in 1.430 CPU sec, 1.435 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 277832, 10, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (296, 307, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (362.56, 162.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (217, 338, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988) [M::mem_pestat] mean and std.dev: (355.11, 170.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1245) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (281, 360, 4036) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11546) [M::mem_pestat] mean and std.dev: (1690.30, 2289.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15301) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 239, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (254.00, 95.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682242 reads in 162.101 CPU sec, 5.230 real sec [M::mem_process_seqs] Processed 681666 reads in 104.502 CPU sec, 3.404 real sec sorting block 105, #reads = 1062430 mem = 536880880 peak = 1180286976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 277820, 4, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 265, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1073) [M::mem_pestat] mean and std.dev: (320.21, 223.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1382) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (217, 338, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985) [M::mem_pestat] mean and std.dev: (354.89, 170.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1241) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 329, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951) [M::mem_pestat] mean and std.dev: (300.14, 135.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1215) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681790 sequences (100000213 bp) in 2.284 CPU sec, 2.400 real sec... [M::process] read 681742 sequences (100000033 bp) in 1.440 CPU sec, 1.439 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 278157, 6, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 253, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (234.03, 117.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (217, 338, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985) [M::mem_pestat] mean and std.dev: (355.36, 171.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1241) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 264, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (279.08, 141.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1170) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681912 reads in 162.712 CPU sec, 5.243 real sec sorting block 106, #reads = 1063255 mem = 536880144 peak = 1180286976 [M::mem_process_seqs] Processed 681798 reads in 104.443 CPU sec, 3.403 real sec sorting block 107, #reads = 1063825 mem = 536877070 peak = 1180286976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 278263, 15, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 189, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955) [M::mem_pestat] mean and std.dev: (285.55, 199.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1217) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (217, 338, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994) [M::mem_pestat] mean and std.dev: (356.63, 172.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1253) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (122, 209, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (162.23, 87.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 303, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (271.97, 92.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682030 sequences (100000042 bp) in 2.388 CPU sec, 2.501 real sec... [M::process] read 681480 sequences (100000075 bp) in 1.430 CPU sec, 1.431 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 277918, 7, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 272, 547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1171) [M::mem_pestat] mean and std.dev: (367.62, 246.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (217, 339, 478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000) [M::mem_pestat] mean and std.dev: (357.13, 172.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1261) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 336, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1218) [M::mem_pestat] mean and std.dev: (387.43, 203.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1565) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681790 reads in 162.770 CPU sec, 5.261 real sec [M::mem_process_seqs] Processed 681742 reads in 103.223 CPU sec, 3.351 real sec sorting block 108, #reads = 1064342 mem = 536877629 peak = 1180286976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 277611, 4, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 251, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012) [M::mem_pestat] mean and std.dev: (324.62, 169.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1269) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 338, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999) [M::mem_pestat] mean and std.dev: (356.76, 173.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1260) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 346, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1233) [M::mem_pestat] mean and std.dev: (312.61, 176.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1587) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681862 sequences (100000088 bp) in 2.163 CPU sec, 2.255 real sec... [M::process] read 687594 sequences (100000066 bp) in 1.470 CPU sec, 1.470 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 277885, 5, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 246, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (285.05, 123.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (219, 343, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017) [M::mem_pestat] mean and std.dev: (361.78, 176.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1283) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 291, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (307.07, 117.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682030 reads in 163.204 CPU sec, 5.257 real sec sorting block 109, #reads = 1062039 mem = 536879164 peak = 1180286976 [M::mem_process_seqs] Processed 681480 reads in 108.384 CPU sec, 3.566 real sec [M::process] read 687308 sequences (100000265 bp) in 2.287 CPU sec, 2.395 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 277754, 9, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 423, 638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1622) [M::mem_pestat] mean and std.dev: (449.62, 350.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2114) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (217, 339, 478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000) [M::mem_pestat] mean and std.dev: (357.95, 174.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1261) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 272, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (243.00, 80.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687288 sequences (100000209 bp) in 1.450 CPU sec, 1.452 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 280754, 18, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 205, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1132) [M::mem_pestat] mean and std.dev: (254.58, 152.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 305, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (328.39, 164.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1175) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (459, 1011, 1865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4677) [M::mem_pestat] mean and std.dev: (1205.71, 875.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6083) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 264, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (285.22, 156.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 279766, 6, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 337, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1099) [M::mem_pestat] mean and std.dev: (327.35, 200.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 304, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (325.49, 162.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1158) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 308, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936) [M::mem_pestat] mean and std.dev: (353.09, 197.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1171) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681862 reads in 270.068 CPU sec, 8.743 real sec sorting block 110, #reads = 1062780 mem = 536877208 peak = 1180286976 [M::mem_process_seqs] Processed 687308 reads in 194.352 CPU sec, 6.243 real sec [M::mem_process_seqs] Processed 687594 reads in 214.854 CPU sec, 6.929 real sec sorting block 111, #reads = 1059747 mem = 536880161 peak = 1180286976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 280498, 7, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 257, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1132) [M::mem_pestat] mean and std.dev: (302.89, 166.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 305, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (327.51, 163.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1167) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 330, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1057) [M::mem_pestat] mean and std.dev: (341.13, 193.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1346) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686136 sequences (100000198 bp) in 2.796 CPU sec, 3.016 real sec... [M::mem_process_seqs] Processed 687288 reads in 99.128 CPU sec, 3.252 real sec sorting block 112, #reads = 1061785 mem = 536879670 peak = 1180286976 [M::process] read 685320 sequences (100000238 bp) in 2.116 CPU sec, 2.270 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 280307, 5, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 226, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (227.70, 100.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 312, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (332.75, 164.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1179) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 324, 499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1099) [M::mem_pestat] mean and std.dev: (313.27, 167.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1399) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685202 sequences (100000277 bp) in 1.489 CPU sec, 1.495 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 280249, 8, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (248, 351, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052) [M::mem_pestat] mean and std.dev: (334.61, 172.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1320) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 318, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953) [M::mem_pestat] mean and std.dev: (339.35, 168.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1203) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 317, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1097) [M::mem_pestat] mean and std.dev: (278.67, 123.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1408) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683936 sequences (100000114 bp) in 1.380 CPU sec, 1.379 real sec... [M::mem_process_seqs] Processed 686136 reads in 168.199 CPU sec, 5.322 real sec [M::mem_process_seqs] Processed 685320 reads in 106.030 CPU sec, 3.419 real sec sorting block 113, #reads = 1067026 mem = 536881990 peak = 1180286976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 280364, 2, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 291, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (298.18, 113.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 319, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956) [M::mem_pestat] mean and std.dev: (339.54, 167.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1207) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 301, 557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1331) [M::mem_pestat] mean and std.dev: (285.12, 192.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1718) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683224 sequences (100000053 bp) in 2.186 CPU sec, 2.329 real sec... [M::process] read 683766 sequences (100000054 bp) in 1.390 CPU sec, 1.384 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 279045, 4, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 348, 579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1355) [M::mem_pestat] mean and std.dev: (311.96, 161.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 324, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949) [M::mem_pestat] mean and std.dev: (341.90, 165.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1196) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 368, 549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1219) [M::mem_pestat] mean and std.dev: (394.33, 204.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1554) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685202 reads in 167.180 CPU sec, 5.402 real sec sorting block 114, #reads = 1069302 mem = 536879976 peak = 1180286976 [M::process] read 683434 sequences (100000184 bp) in 1.580 CPU sec, 1.582 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 279241, 7, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 407, 653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1673) [M::mem_pestat] mean and std.dev: (420.50, 301.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2183) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 327, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952) [M::mem_pestat] mean and std.dev: (345.02, 166.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1199) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 291, 814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1982) [M::mem_pestat] mean and std.dev: (510.58, 405.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2566) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683224 reads in 88.768 CPU sec, 2.951 real sec [M::mem_process_seqs] Processed 683936 reads in 219.565 CPU sec, 7.131 real sec sorting block 115, #reads = 1069198 mem = 536878171 peak = 1180286976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 278754, 5, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 354, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (295.14, 125.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 323, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (341.76, 166.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1192) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 397, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (316.81, 140.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683316 sequences (100000141 bp) in 2.281 CPU sec, 2.513 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 280117, 11, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 185, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (249.42, 132.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1078) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 327, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952) [M::mem_pestat] mean and std.dev: (344.83, 166.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1199) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1581, 1776, 1776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1191, 2166) [M::mem_pestat] mean and std.dev: (1711.00, 91.92) [M::mem_pestat] low and high boundaries for proper pairs: (996, 2361) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (143, 291, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1253) [M::mem_pestat] mean and std.dev: (309.72, 210.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1623) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683270 sequences (100000279 bp) in 1.420 CPU sec, 1.417 real sec... [M::mem_process_seqs] Processed 683766 reads in 180.279 CPU sec, 5.773 real sec [M::mem_process_seqs] Processed 683434 reads in 102.823 CPU sec, 3.331 real sec sorting block 116, #reads = 1067676 mem = 536881314 peak = 1180286976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 279105, 8, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 256, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (261.89, 126.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 327, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943) [M::mem_pestat] mean and std.dev: (343.00, 164.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1187) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 342, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1096) [M::mem_pestat] mean and std.dev: (302.05, 143.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1407) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683304 sequences (100000125 bp) in 2.422 CPU sec, 2.577 real sec... [M::process] read 683118 sequences (100000088 bp) in 1.430 CPU sec, 1.424 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 279177, 9, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 217, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961) [M::mem_pestat] mean and std.dev: (227.12, 103.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1233) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 326, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943) [M::mem_pestat] mean and std.dev: (342.77, 163.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1187) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 286, 515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1119) [M::mem_pestat] mean and std.dev: (318.76, 145.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1421) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683316 reads in 168.170 CPU sec, 5.420 real sec sorting block 117, #reads = 1068682 mem = 536878339 peak = 1180286976 [M::mem_process_seqs] Processed 683270 reads in 105.218 CPU sec, 3.388 real sec sorting block 118, #reads = 1069780 mem = 536877824 peak = 1180286976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 278959, 5, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 197, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (293.91, 161.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 327, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943) [M::mem_pestat] mean and std.dev: (343.46, 164.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1187) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (333, 451, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (71, 726) [M::mem_pestat] mean and std.dev: (370.08, 131.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683290 sequences (100000055 bp) in 1.979 CPU sec, 2.084 real sec... [M::process] read 683604 sequences (100000132 bp) in 1.420 CPU sec, 1.420 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 279324, 7, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 355, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956) [M::mem_pestat] mean and std.dev: (382.68, 204.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1202) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 329, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953) [M::mem_pestat] mean and std.dev: (345.36, 165.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1200) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 348, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (314.68, 118.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683304 reads in 163.995 CPU sec, 5.268 real sec [M::mem_process_seqs] Processed 683118 reads in 103.524 CPU sec, 3.369 real sec sorting block 119, #reads = 1066723 mem = 536880901 peak = 1180286976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 278922, 9, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 316, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054) [M::mem_pestat] mean and std.dev: (261.52, 109.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1346) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 327, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954) [M::mem_pestat] mean and std.dev: (344.22, 165.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1202) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (247, 359, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (347.06, 130.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1063) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683890 sequences (100000060 bp) in 2.285 CPU sec, 2.402 real sec... [M::process] read 683986 sequences (100000257 bp) in 1.460 CPU sec, 1.459 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 279023, 7, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 270, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045) [M::mem_pestat] mean and std.dev: (327.40, 201.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1331) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 324, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (341.25, 164.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1189) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 262, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (250.04, 85.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683290 reads in 167.642 CPU sec, 5.404 real sec [M::mem_process_seqs] Processed 683604 reads in 106.871 CPU sec, 3.395 real sec sorting block 120, #reads = 1067257 mem = 536880349 peak = 1180286976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 279275, 9, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 282, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1104) [M::mem_pestat] mean and std.dev: (300.09, 169.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 321, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (339.23, 164.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1190) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (137, 265, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (232.33, 118.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684206 sequences (100000200 bp) in 2.467 CPU sec, 2.590 real sec... [M::process] read 682588 sequences (100000166 bp) in 1.460 CPU sec, 1.465 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 278842, 7, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 284, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (291.03, 135.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1030) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 320, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (338.98, 163.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1183) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 216, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (222.61, 79.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683890 reads in 169.398 CPU sec, 5.459 real sec sorting block 121, #reads = 1066404 mem = 536881276 peak = 1180286976 [M::mem_process_seqs] Processed 683986 reads in 110.215 CPU sec, 3.555 real sec sorting block 122, #reads = 1066502 mem = 536881181 peak = 1180286976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 279437, 7, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 347, 574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1280) [M::mem_pestat] mean and std.dev: (337.05, 200.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 323, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951) [M::mem_pestat] mean and std.dev: (341.34, 165.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1199) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 286, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (301.52, 130.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682646 sequences (100000183 bp) in 2.449 CPU sec, 2.559 real sec... [M::process] read 682314 sequences (100000232 bp) in 1.760 CPU sec, 1.757 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 278936, 8, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (289, 491, 1063) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2611) [M::mem_pestat] mean and std.dev: (642.47, 539.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3385) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 332, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978) [M::mem_pestat] mean and std.dev: (351.31, 170.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1233) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 239, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (99, 364) [M::mem_pestat] mean and std.dev: (220.06, 44.11) [M::mem_pestat] low and high boundaries for proper pairs: (44, 417) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684206 reads in 177.688 CPU sec, 5.690 real sec [M::mem_process_seqs] Processed 682588 reads in 112.950 CPU sec, 3.679 real sec sorting block 123, #reads = 1065899 mem = 536879075 peak = 1180286976 [M::process] read 682518 sequences (100000170 bp) in 2.099 CPU sec, 2.208 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 278851, 10, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 278, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996) [M::mem_pestat] mean and std.dev: (285.41, 133.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1274) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 334, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982) [M::mem_pestat] mean and std.dev: (352.74, 171.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1238) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (615, 1059, 2215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5415) [M::mem_pestat] mean and std.dev: (1514.50, 1026.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7015) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 344, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (322.20, 136.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682646 reads in 94.019 CPU sec, 3.131 real sec [M::process] read 681904 sequences (100000218 bp) in 1.838 CPU sec, 1.957 real sec... sorting block 124, #reads = 1066294 mem = 536887701 peak = 1180286976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 278348, 5, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (278, 387, 652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1400) [M::mem_pestat] mean and std.dev: (431.91, 237.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1774) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 335, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983) [M::mem_pestat] mean and std.dev: (352.66, 170.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1239) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 282, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (306.03, 151.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682058 sequences (100000038 bp) in 1.912 CPU sec, 1.915 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 278893, 13, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 237, 592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1370) [M::mem_pestat] mean and std.dev: (321.30, 185.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1759) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 335, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977) [M::mem_pestat] mean and std.dev: (351.77, 169.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1231) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (430, 832, 1022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2206) [M::mem_pestat] mean and std.dev: (846.00, 483.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2798) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 350, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1164) [M::mem_pestat] mean and std.dev: (302.00, 132.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1481) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682314 reads in 192.375 CPU sec, 6.154 real sec [M::mem_process_seqs] Processed 682518 reads in 207.979 CPU sec, 6.750 real sec sorting block 125, #reads = 1064250 mem = 536881876 peak = 1180286976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278041, 8, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (244, 287, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (277.27, 93.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (217, 337, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982) [M::mem_pestat] mean and std.dev: (354.99, 171.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1237) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 264, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (241.87, 103.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683444 sequences (100000278 bp) in 2.224 CPU sec, 2.381 real sec... [M::process] read 681966 sequences (100000030 bp) in 1.470 CPU sec, 1.471 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 278253, 10, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 236, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (39, 444) [M::mem_pestat] mean and std.dev: (218.83, 78.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (217, 338, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991) [M::mem_pestat] mean and std.dev: (356.29, 173.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1249) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (423, 3896, 3896) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10842) [M::mem_pestat] mean and std.dev: (3413.80, 3142.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15982) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 365, 573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1283) [M::mem_pestat] mean and std.dev: (385.55, 214.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1638) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681904 reads in 177.794 CPU sec, 5.747 real sec [M::mem_process_seqs] Processed 682058 reads in 114.380 CPU sec, 3.645 real sec sorting block 126, #reads = 1064414 mem = 536879184 peak = 1180286976 [M::process] read 685420 sequences (100000283 bp) in 2.190 CPU sec, 2.307 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 278477, 14, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 273, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (263.33, 114.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 326, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975) [M::mem_pestat] mean and std.dev: (348.47, 172.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1230) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (150, 560, 667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1701) [M::mem_pestat] mean and std.dev: (368.83, 287.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2218) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 240, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (279.86, 107.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1022) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683444 reads in 104.713 CPU sec, 3.473 real sec sorting block 127, #reads = 1063937 mem = 536877933 peak = 1180286976 [M::process] read 689954 sequences (100000095 bp) in 1.936 CPU sec, 2.040 real sec... [M::process] read 689624 sequences (100000029 bp) in 1.541 CPU sec, 1.545 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 278905, 12, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 300, 1006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2690) [M::mem_pestat] mean and std.dev: (462.12, 457.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 317, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961) [M::mem_pestat] mean and std.dev: (339.41, 169.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1214) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (918, 918, 1520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2724) [M::mem_pestat] mean and std.dev: (1059.83, 648.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3653) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 265, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (282.98, 150.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1159) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 278188, 15, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (239, 359, 587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1283) [M::mem_pestat] mean and std.dev: (409.78, 251.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 335, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995) [M::mem_pestat] mean and std.dev: (355.95, 175.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1255) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (98, 949, 1200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3404) [M::mem_pestat] mean and std.dev: (598.69, 617.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4506) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (275, 338, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971) [M::mem_pestat] mean and std.dev: (368.41, 169.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1203) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685420 reads in 220.875 CPU sec, 7.094 real sec [M::mem_process_seqs] Processed 681966 reads in 242.226 CPU sec, 7.796 real sec sorting block 128, #reads = 1055644 mem = 536883369 peak = 1180286976 [M::process] read 690492 sequences (100000188 bp) in 2.093 CPU sec, 2.192 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 281359, 9, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 311, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1125) [M::mem_pestat] mean and std.dev: (361.76, 228.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 289, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (312.32, 155.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 283, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (304.78, 167.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1170) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689076 sequences (100000063 bp) in 1.529 CPU sec, 1.530 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 282036, 3, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 227, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023) [M::mem_pestat] mean and std.dev: (293.45, 156.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1310) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 288, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (309.09, 152.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 318, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (310.32, 155.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 281128, 10, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 204, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (259.23, 143.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 292, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (314.26, 155.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (128, 698, 1411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3977) [M::mem_pestat] mean and std.dev: (748.67, 646.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5260) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 300, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (310.31, 154.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1158) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689954 reads in 267.115 CPU sec, 8.539 real sec sorting block 129, #reads = 1060753 mem = 536881907 peak = 1180286976 [M::mem_process_seqs] Processed 690492 reads in 202.186 CPU sec, 6.481 real sec [M::mem_process_seqs] Processed 689624 reads in 223.544 CPU sec, 7.182 real sec sorting block 130, #reads = 1062395 mem = 536879831 peak = 1180286976 [M::process] read 688870 sequences (100000144 bp) in 2.824 CPU sec, 3.027 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 281438, 15, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 266, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (288.32, 159.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 295, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (315.39, 154.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (630, 1369, 2186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5298) [M::mem_pestat] mean and std.dev: (1232.67, 730.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6854) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 210, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (244.17, 139.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688352 sequences (100000238 bp) in 1.699 CPU sec, 1.745 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 281378, 12, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 360, 680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1670) [M::mem_pestat] mean and std.dev: (415.70, 283.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2165) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 295, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (315.40, 154.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (289, 1123, 3087) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8683) [M::mem_pestat] mean and std.dev: (1393.33, 1256.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11481) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (264, 378, 676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1500) [M::mem_pestat] mean and std.dev: (376.60, 186.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1912) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686502 sequences (100000165 bp) in 1.420 CPU sec, 1.419 real sec... [M::mem_process_seqs] Processed 689076 reads in 191.634 CPU sec, 6.146 real sec sorting block 131, #reads = 1062404 mem = 536878478 peak = 1180286976 [M::mem_process_seqs] Processed 688870 reads in 125.650 CPU sec, 4.127 real sec [M::process] read 685544 sequences (100000070 bp) in 2.540 CPU sec, 2.694 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 280750, 12, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 252, 667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1677) [M::mem_pestat] mean and std.dev: (357.97, 320.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2182) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 298, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (316.51, 152.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1532, 2589, 2589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4703) [M::mem_pestat] mean and std.dev: (1984.09, 1156.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6611) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 260, 628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1458) [M::mem_pestat] mean and std.dev: (370.98, 274.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1873) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685248 sequences (100000090 bp) in 1.870 CPU sec, 1.871 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 280248, 8, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 246, 650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1600) [M::mem_pestat] mean and std.dev: (329.33, 254.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2075) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 308, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (325.62, 156.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 229, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (225.12, 84.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688352 reads in 190.322 CPU sec, 6.150 real sec sorting block 132, #reads = 1061541 mem = 536881062 peak = 1180286976 [M::mem_process_seqs] Processed 686502 reads in 119.843 CPU sec, 3.895 real sec sorting block 133, #reads = 1061519 mem = 536884865 peak = 1180286976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 279543, 7, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (317, 414, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (336.64, 121.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 314, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (331.96, 159.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1169) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (248, 341, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (329.26, 127.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684770 sequences (100000277 bp) in 2.289 CPU sec, 2.381 real sec... [M::process] read 684492 sequences (100000192 bp) in 1.870 CPU sec, 1.870 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 280337, 7, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 293, 1845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5141) [M::mem_pestat] mean and std.dev: (897.71, 1133.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 318, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (334.82, 161.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1183) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 252, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (257.44, 103.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685544 reads in 182.128 CPU sec, 5.865 real sec [M::mem_process_seqs] Processed 685248 reads in 117.270 CPU sec, 3.755 real sec sorting block 134, #reads = 1063955 mem = 536882233 peak = 1180286976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 279290, 7, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 296, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (293.86, 135.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 320, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945) [M::mem_pestat] mean and std.dev: (336.57, 161.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1192) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 210, 639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1549) [M::mem_pestat] mean and std.dev: (384.50, 253.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2004) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684384 sequences (100000159 bp) in 2.280 CPU sec, 2.411 real sec... [M::process] read 684504 sequences (100000045 bp) in 1.440 CPU sec, 1.437 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 279565, 12, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 323, 573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1299) [M::mem_pestat] mean and std.dev: (321.67, 153.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 321, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (338.37, 163.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1190) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (258, 258, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (287.82, 81.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (273, 328, 584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1206) [M::mem_pestat] mean and std.dev: (360.98, 151.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1517) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684770 reads in 183.562 CPU sec, 5.884 real sec [M::mem_process_seqs] Processed 684492 reads in 116.961 CPU sec, 3.745 real sec sorting block 135, #reads = 1064685 mem = 536883241 peak = 1180286976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 279409, 12, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 306, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (293.16, 151.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1026) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 322, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (338.73, 162.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1190) [M::process] read 684186 sequences (100000053 bp) in 2.151 CPU sec, 2.263 real sec... [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1113, 1405, 1853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3333) [M::mem_pestat] mean and std.dev: (1128.30, 623.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4073) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 234, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (262.49, 107.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683420 sequences (100000132 bp) in 1.630 CPU sec, 1.729 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 278811, 7, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 245, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (245.43, 102.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 324, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965) [M::mem_pestat] mean and std.dev: (342.02, 166.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1218) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 237, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (248.56, 102.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684384 reads in 179.220 CPU sec, 5.723 real sec sorting block 136, #reads = 1064741 mem = 536880493 peak = 1180286976 [M::mem_process_seqs] Processed 684504 reads in 117.522 CPU sec, 3.842 real sec sorting block 137, #reads = 1064260 mem = 536878250 peak = 1180286976 [M::process] read 683398 sequences (100000069 bp) in 2.128 CPU sec, 2.244 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 279155, 9, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 260, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (244.67, 87.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 326, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964) [M::mem_pestat] mean and std.dev: (343.27, 165.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1216) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 296, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (287.06, 128.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683126 sequences (100000219 bp) in 1.488 CPU sec, 1.485 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 278933, 12, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 281, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (278.56, 141.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 329, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966) [M::mem_pestat] mean and std.dev: (345.59, 166.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1218) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (925, 1213, 9085) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 25405) [M::mem_pestat] mean and std.dev: (2932.25, 3581.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 33565) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 243, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (237.87, 79.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 278883, 6, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 213, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (191.50, 61.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 330, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961) [M::mem_pestat] mean and std.dev: (345.92, 165.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1211) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 261, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (290.36, 152.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684186 reads in 259.816 CPU sec, 8.405 real sec [M::mem_process_seqs] Processed 683420 reads in 193.271 CPU sec, 6.198 real sec [M::mem_process_seqs] Processed 683398 reads in 212.973 CPU sec, 6.853 real sec sorting block 138, #reads = 1064572 mem = 536880922 peak = 1180286976 [M::process] read 682880 sequences (100000071 bp) in 2.563 CPU sec, 2.740 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 278874, 14, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 279, 630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1538) [M::mem_pestat] mean and std.dev: (453.24, 431.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2178) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 330, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953) [M::mem_pestat] mean and std.dev: (345.10, 163.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1200) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (54, 579, 579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1629) [M::mem_pestat] mean and std.dev: (401.69, 347.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2154) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 291, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810) [M::mem_pestat] mean and std.dev: (278.97, 100.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1009) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682586 sequences (100000246 bp) in 1.798 CPU sec, 1.878 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 277811, 10, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 355, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (316.38, 153.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 332, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963) [M::mem_pestat] mean and std.dev: (347.57, 165.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1213) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (105, 496, 3829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11277) [M::mem_pestat] mean and std.dev: (1365.80, 1624.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15001) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 296, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (278.69, 119.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683126 reads in 175.736 CPU sec, 5.627 real sec [M::process] read 682226 sequences (100000290 bp) in 1.412 CPU sec, 1.423 real sec... sorting block 139, #reads = 1063732 mem = 536880486 peak = 1180286976 [M::mem_process_seqs] Processed 682880 reads in 116.380 CPU sec, 3.910 real sec sorting block 140, #reads = 1065132 mem = 536879850 peak = 1192484864 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 278490, 12, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 304, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1098) [M::mem_pestat] mean and std.dev: (346.85, 220.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 335, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971) [M::mem_pestat] mean and std.dev: (350.55, 167.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1223) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (315, 315, 1164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2862) [M::mem_pestat] mean and std.dev: (505.73, 411.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3711) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (262, 385, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075) [M::mem_pestat] mean and std.dev: (394.15, 170.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1346) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681616 sequences (100000068 bp) in 2.415 CPU sec, 2.576 real sec... [M::process] read 681844 sequences (100000298 bp) in 1.810 CPU sec, 1.815 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278285, 14, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 254, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (294.44, 170.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (218, 339, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974) [M::mem_pestat] mean and std.dev: (353.43, 167.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1226) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (164, 784, 1831) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5165) [M::mem_pestat] mean and std.dev: (1237.64, 1156.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6832) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 376, 699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1667) [M::mem_pestat] mean and std.dev: (399.22, 278.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2151) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682586 reads in 176.793 CPU sec, 5.812 real sec [M::mem_process_seqs] Processed 682226 reads in 113.350 CPU sec, 3.665 real sec sorting block 141, #reads = 1066056 mem = 536877942 peak = 1192484864 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278371, 13, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 283, 686) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1688) [M::mem_pestat] mean and std.dev: (346.69, 287.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2189) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (219, 340, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978) [M::mem_pestat] mean and std.dev: (355.21, 168.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1231) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 388, 2589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7731) [M::mem_pestat] mean and std.dev: (1095.77, 1205.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10302) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 291, 582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1364) [M::mem_pestat] mean and std.dev: (327.49, 179.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1755) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682078 sequences (100000147 bp) in 2.367 CPU sec, 2.468 real sec... [M::process] read 681172 sequences (100000027 bp) in 1.840 CPU sec, 1.846 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 278097, 13, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 244, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1067) [M::mem_pestat] mean and std.dev: (279.35, 165.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1359) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (218, 339, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977) [M::mem_pestat] mean and std.dev: (353.82, 167.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1230) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1514, 2249, 2459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4349) [M::mem_pestat] mean and std.dev: (2098.54, 812.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5347) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 468, 569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1227) [M::mem_pestat] mean and std.dev: (374.13, 159.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1556) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681616 reads in 173.940 CPU sec, 5.592 real sec [M::mem_process_seqs] Processed 681844 reads in 110.951 CPU sec, 3.516 real sec sorting block 142, #reads = 1064719 mem = 536880855 peak = 1192484864 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 278258, 8, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 220, 573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1449) [M::mem_pestat] mean and std.dev: (351.16, 323.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1887) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (217, 335, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955) [M::mem_pestat] mean and std.dev: (349.81, 165.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1201) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 245, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (284.37, 93.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681244 sequences (100000195 bp) in 2.314 CPU sec, 2.435 real sec... [M::process] read 349668 sequences (51240563 bp) in 1.100 CPU sec, 1.107 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 277980, 13, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 274, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042) [M::mem_pestat] mean and std.dev: (245.65, 115.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1341) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (221, 340, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968) [M::mem_pestat] mean and std.dev: (354.84, 167.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1217) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (281, 733, 4429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12725) [M::mem_pestat] mean and std.dev: (1858.15, 1822.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16873) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 271, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (317.19, 169.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1150) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682078 reads in 162.758 CPU sec, 5.194 real sec sorting block 143, #reads = 1065542 mem = 536883825 peak = 1192484864 [M::mem_process_seqs] Processed 681172 reads in 101.207 CPU sec, 3.308 real sec sorting block 144, #reads = 1065313 mem = 536874513 peak = 1214488576 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 278505, 7, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 367, 583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1381) [M::mem_pestat] mean and std.dev: (409.76, 303.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (221, 339, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962) [M::mem_pestat] mean and std.dev: (354.29, 167.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1209) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 302, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797) [M::mem_pestat] mean and std.dev: (339.44, 211.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1185) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681244 reads in 85.734 CPU sec, 2.911 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 142433, 3, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 181, 631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1667) [M::mem_pestat] mean and std.dev: (301.55, 334.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2185) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 333, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953) [M::mem_pestat] mean and std.dev: (348.58, 166.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1199) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 214, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (199.00, 69.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 349668 reads in 65.273 CPU sec, 2.306 real sec sorting block 145, #reads = 1065300 mem = 536881766 peak = 1214488576 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 154667390 overall: 70228652032 mem 25984.804 user 664.365 sys 887.080 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1029_FZX-T1-TDNA-1_A23MHWWLT4_1.L006\tPU:L006\tSM:1029_FZX-T1-TDNA-1_A23MHWWLT4_1\tLB:1029_FZX-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1029_FZX-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 1029_FZX-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 887.120 sec; CPU: 26649.172 sec sorting block 146, #reads = 105055 mem = 53438946 peak = 1214488576 uniq header count 1 merging 147 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 9073238559 output reads: 155772413 bam_mem_sort: 147 calls 92.474 user 1.818 sys 96.217 real bam_write: 147 calls 67.934 user 4.172 sys 123.117 real execute: 1 calls 38.389 user 2.028 sys 938.858 real merge_files: 1 calls 38.370 user 2.001 sys 51.596 real parse_chunk: 2338 calls 102.569 user 28.142 sys 148.741 real read_chunk: 6313 calls 47.779 user 38.193 sys 811.067 real sort_block: 1 calls 34.889 user 37.518 sys 789.795 real write_chunk: 3973 calls 37.904 user 1.665 sys 50.487 real overall: 3528679424 mem 2072.071 user 94.322 sys 938.878 real ls: cannot access '*.cram': No such file or directory