cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1029_N5D-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1616.094 CPU sec, 413.489 real sec
[M::process] read 680942 sequences (100000198 bp) in 0.946 CPU sec, 0.948 real sec...
[M::process] read 681234 sequences (100000022 bp) in 1.168 CPU sec, 1.206 real sec...
[M::process] read 682542 sequences (100000141 bp) in 1.200 CPU sec, 1.196 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 278085, 6, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 218, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610)
[M::mem_pestat] mean and std.dev: (219.86, 87.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 354, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (367.28, 173.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 281, 587)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1393)
[M::mem_pestat] mean and std.dev: (394.14, 275.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1796)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680094 sequences (100000223 bp) in 1.119 CPU sec, 1.125 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 277974, 7, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 266, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (264.50, 99.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 342, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (358.73, 173.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 347, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (343.61, 132.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 278498, 4, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 274, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (285.93, 144.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 978)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 353, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (366.97, 174.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 281, 2548)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7258)
[M::mem_pestat] mean and std.dev: (941.46, 1035.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9613)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680942 reads in 253.768 CPU sec, 8.291 real sec
[M::mem_process_seqs] Processed 682542 reads in 171.869 CPU sec, 5.751 real sec
[M::mem_process_seqs] Processed 681234 reads in 187.211 CPU sec, 6.248 real sec
sorting block 0, #reads = 1069688 mem = 536880055 peak = 584671232
[M::process] read 681810 sequences (100000185 bp) in 1.962 CPU sec, 2.174 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 277776, 6, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (227, 320, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (328.92, 189.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 362, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (375.14, 177.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 308, 600)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1430)
[M::mem_pestat] mean and std.dev: (298.43, 156.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1845)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681302 sequences (100000047 bp) in 1.249 CPU sec, 1.261 real sec...
[M::process] read 680680 sequences (100000262 bp) in 1.160 CPU sec, 1.164 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 277754, 3, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 245, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (246.45, 116.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 853)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 347, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (362.82, 174.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 264, 596)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1424)
[M::mem_pestat] mean and std.dev: (341.41, 238.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1838)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680094 reads in 153.161 CPU sec, 4.915 real sec
sorting block 1, #reads = 1070095 mem = 536880111 peak = 860499968
[M::mem_process_seqs] Processed 681810 reads in 99.140 CPU sec, 3.172 real sec
sorting block 2, #reads = 1069971 mem = 536876830 peak = 956162048
[M::process] read 680600 sequences (100000087 bp) in 1.539 CPU sec, 1.636 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 278079, 6, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 317, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (310.15, 157.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 347, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (363.04, 173.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 358, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051)
[M::mem_pestat] mean and std.dev: (349.07, 162.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1326)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681118 sequences (100000225 bp) in 1.240 CPU sec, 1.240 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 277446, 5, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 253, 637)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1579)
[M::mem_pestat] mean and std.dev: (337.96, 286.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2050)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 348, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (363.31, 171.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 355, 588)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1358)
[M::mem_pestat] mean and std.dev: (352.92, 196.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1743)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 277785, 1, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (111, 245, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (227.78, 127.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 349, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (364.55, 172.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 259, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (234.76, 82.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681302 reads in 219.633 CPU sec, 7.038 real sec
[M::mem_process_seqs] Processed 680680 reads in 164.127 CPU sec, 5.289 real sec
[M::mem_process_seqs] Processed 680600 reads in 179.447 CPU sec, 5.801 real sec
sorting block 3, #reads = 1069758 mem = 536876194 peak = 956162048
[M::process] read 679886 sequences (100000174 bp) in 1.824 CPU sec, 1.968 real sec...
sorting block 4, #reads = 1069265 mem = 536879478 peak = 956162048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 277504, 9, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 232, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (261.69, 131.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 343, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (359.20, 171.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 306, 703)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1783)
[M::mem_pestat] mean and std.dev: (321.07, 201.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2323)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680948 sequences (100000190 bp) in 1.451 CPU sec, 1.500 real sec...
[M::process] read 680774 sequences (100000092 bp) in 1.210 CPU sec, 1.210 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 277068, 11, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 254, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864)
[M::mem_pestat] mean and std.dev: (264.35, 126.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1098)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 351, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (365.42, 171.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (388, 735, 3290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9094)
[M::mem_pestat] mean and std.dev: (1431.36, 1393.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11996)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 289, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (312.19, 135.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681118 reads in 156.547 CPU sec, 4.995 real sec
[M::mem_process_seqs] Processed 679886 reads in 99.631 CPU sec, 3.170 real sec
sorting block 5, #reads = 1069382 mem = 536878413 peak = 956162048
[M::process] read 680278 sequences (100000090 bp) in 1.768 CPU sec, 1.884 real sec...
[M::process] read 681212 sequences (100000269 bp) in 1.250 CPU sec, 1.253 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 277324, 7, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 292, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1089)
[M::mem_pestat] mean and std.dev: (287.78, 125.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1383)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 346, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (363.61, 174.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 272, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (253.03, 103.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 877)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680774 reads in 82.633 CPU sec, 2.643 real sec
sorting block 6, #reads = 1069601 mem = 536878979 peak = 956162048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 278299, 3, 53)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 201, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562)
[M::mem_pestat] mean and std.dev: (216.00, 88.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 699)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 343, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (359.98, 171.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 267, 621)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1421)
[M::mem_pestat] mean and std.dev: (403.02, 274.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1821)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681554 sequences (100000001 bp) in 1.282 CPU sec, 1.284 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 277602, 9, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693)
[M::mem_pestat] mean and std.dev: (247.09, 118.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 868)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 354, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (369.86, 176.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 309, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (340.29, 164.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1100)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680948 reads in 234.424 CPU sec, 7.492 real sec
[M::mem_process_seqs] Processed 680278 reads in 179.731 CPU sec, 5.758 real sec
sorting block 7, #reads = 1070548 mem = 536880489 peak = 956162048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 278110, 3, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 276, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1065)
[M::mem_pestat] mean and std.dev: (280.35, 159.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1365)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 352, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (365.98, 174.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 264, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (307.31, 117.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681532 sequences (100000221 bp) in 1.934 CPU sec, 2.080 real sec...
[M::process] read 680370 sequences (100000257 bp) in 1.290 CPU sec, 1.289 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 277785, 11, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 384, 1130)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3082)
[M::mem_pestat] mean and std.dev: (530.85, 505.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4058)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 352, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (367.15, 176.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (640, 656, 1269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2527)
[M::mem_pestat] mean and std.dev: (916.00, 462.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3156)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 245, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (264.39, 113.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681212 reads in 147.744 CPU sec, 4.721 real sec
[M::mem_process_seqs] Processed 681554 reads in 94.454 CPU sec, 3.020 real sec
sorting block 8, #reads = 1070491 mem = 536883834 peak = 956162048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 278532, 8, 56)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (203, 319, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1097)
[M::mem_pestat] mean and std.dev: (379.81, 210.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1395)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 350, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (365.35, 175.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 231, 292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504)
[M::mem_pestat] mean and std.dev: (234.19, 81.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 610)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681508 sequences (100000200 bp) in 1.808 CPU sec, 1.923 real sec...
[M::process] read 680878 sequences (100000147 bp) in 1.310 CPU sec, 1.314 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 277892, 10, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 195, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (224.00, 94.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 357, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (371.28, 176.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (537, 2136, 2136)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5334)
[M::mem_pestat] mean and std.dev: (1550.80, 1247.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6933)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 307, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (295.17, 127.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681532 reads in 146.662 CPU sec, 4.692 real sec
[M::mem_process_seqs] Processed 680370 reads in 94.011 CPU sec, 3.006 real sec
sorting block 9, #reads = 1072003 mem = 536878707 peak = 956162048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 278463, 9, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 373, 940)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2514)
[M::mem_pestat] mean and std.dev: (550.34, 568.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3301)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 350, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (365.16, 175.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 237, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614)
[M::mem_pestat] mean and std.dev: (252.94, 88.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 679784 sequences (100000274 bp) in 1.909 CPU sec, 2.036 real sec...
[M::process] read 681118 sequences (100000011 bp) in 1.340 CPU sec, 1.339 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 278332, 5, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 224, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (248.85, 118.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 352, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (366.71, 174.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (135, 228, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1284)
[M::mem_pestat] mean and std.dev: (323.03, 224.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1667)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681508 reads in 148.456 CPU sec, 4.745 real sec
sorting block 10, #reads = 1072089 mem = 536880342 peak = 956162048
[M::mem_process_seqs] Processed 680878 reads in 94.894 CPU sec, 3.027 real sec
sorting block 11, #reads = 1071957 mem = 536879226 peak = 956162048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 277993, 4, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 299, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (279.41, 177.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 357, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (371.72, 175.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 244, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042)
[M::mem_pestat] mean and std.dev: (310.50, 209.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1329)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681146 sequences (100000255 bp) in 2.232 CPU sec, 2.362 real sec...
[M::process] read 680174 sequences (100000039 bp) in 1.340 CPU sec, 1.338 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 277406, 7, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (203, 234, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626)
[M::mem_pestat] mean and std.dev: (268.05, 124.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 348, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (364.56, 175.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 271, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (313.65, 161.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 679784 reads in 149.807 CPU sec, 4.783 real sec
[M::mem_process_seqs] Processed 681118 reads in 96.593 CPU sec, 3.085 real sec
sorting block 12, #reads = 1070642 mem = 536877792 peak = 956162048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 278187, 5, 48)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (203, 243, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (286.60, 146.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 345, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (362.47, 174.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 371, 597)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1363)
[M::mem_pestat] mean and std.dev: (408.31, 266.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1746)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681222 sequences (100000124 bp) in 1.978 CPU sec, 2.120 real sec...
[M::process] read 681350 sequences (100000095 bp) in 1.370 CPU sec, 1.370 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 277974, 5, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 308, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (283.28, 106.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 351, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (367.53, 175.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 283, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (293.75, 128.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681146 reads in 149.044 CPU sec, 4.762 real sec
[M::mem_process_seqs] Processed 680174 reads in 95.243 CPU sec, 3.039 real sec
sorting block 13, #reads = 1070266 mem = 536880659 peak = 956162048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 278503, 4, 54)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 384, 1358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3742)
[M::mem_pestat] mean and std.dev: (577.59, 528.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4934)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 343, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (360.58, 173.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 310, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684)
[M::mem_pestat] mean and std.dev: (301.13, 126.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681850 sequences (100000015 bp) in 2.118 CPU sec, 2.270 real sec...
[M::process] read 682772 sequences (100000039 bp) in 1.410 CPU sec, 1.406 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 278970, 6, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 250, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.60, 107.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 346, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (364.08, 176.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 364, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (298.05, 115.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681222 reads in 148.704 CPU sec, 4.743 real sec
sorting block 14, #reads = 1070093 mem = 536880703 peak = 956162048
[M::mem_process_seqs] Processed 681350 reads in 95.033 CPU sec, 3.033 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 278454, 4, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 421, 568)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1354)
[M::mem_pestat] mean and std.dev: (353.66, 257.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1747)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 344, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (361.28, 174.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (302, 326, 587)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1157)
[M::mem_pestat] mean and std.dev: (418.61, 280.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1539)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681554 sequences (100000158 bp) in 2.100 CPU sec, 2.277 real sec...
[M::process] read 680170 sequences (100000298 bp) in 1.410 CPU sec, 1.402 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278274, 6, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 291, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (243.57, 128.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 339, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (352.68, 167.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 312, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (291.73, 94.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681850 reads in 153.875 CPU sec, 4.921 real sec
sorting block 15, #reads = 1071040 mem = 536877871 peak = 956162048
[M::mem_process_seqs] Processed 682772 reads in 100.045 CPU sec, 3.185 real sec
sorting block 16, #reads = 1068836 mem = 536880149 peak = 990244864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 278417, 3, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 269, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (220.64, 93.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 345, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (358.60, 168.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 276, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (278.14, 109.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1318)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681994 sequences (100000124 bp) in 2.438 CPU sec, 2.607 real sec...
[M::process] read 681598 sequences (100000206 bp) in 1.430 CPU sec, 1.426 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 277943, 11, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 292, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (314.00, 180.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 359, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (369.93, 172.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (938, 1108, 2049)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4271)
[M::mem_pestat] mean and std.dev: (1303.09, 782.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5382)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 337, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (332.87, 153.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681554 reads in 152.957 CPU sec, 4.882 real sec
[M::mem_process_seqs] Processed 680170 reads in 97.862 CPU sec, 3.117 real sec
sorting block 17, #reads = 1069369 mem = 536878736 peak = 990244864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 278326, 13, 55)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 309, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (252.46, 84.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 346, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (359.78, 171.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (927, 1423, 3004)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7158)
[M::mem_pestat] mean and std.dev: (1393.58, 839.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9235)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 308, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (297.56, 136.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 680500 sequences (100000166 bp) in 2.085 CPU sec, 2.241 real sec...
[M::process] read 680372 sequences (100000243 bp) in 1.410 CPU sec, 1.411 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 278434, 1, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 265, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (250.11, 102.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 346, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (360.20, 170.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 186, 235)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399)
[M::mem_pestat] mean and std.dev: (188.11, 64.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 481)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681994 reads in 153.573 CPU sec, 4.912 real sec
[M::mem_process_seqs] Processed 681598 reads in 98.440 CPU sec, 3.142 real sec
sorting block 18, #reads = 1071594 mem = 536882829 peak = 990244864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 277883, 6, 72)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 261, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1119)
[M::mem_pestat] mean and std.dev: (295.91, 176.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1436)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 354, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (367.37, 173.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 272, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (275.77, 96.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681250 sequences (100000294 bp) in 2.333 CPU sec, 2.481 real sec...
[M::process] read 681140 sequences (100000216 bp) in 1.400 CPU sec, 1.403 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 277398, 2, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 291, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1117)
[M::mem_pestat] mean and std.dev: (301.97, 190.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1422)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 353, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (366.48, 172.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 219, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (36, 406)
[M::mem_pestat] mean and std.dev: (207.19, 46.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 480)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680500 reads in 153.469 CPU sec, 4.891 real sec
sorting block 19, #reads = 1070632 mem = 536881325 peak = 990244864
[M::mem_process_seqs] Processed 680372 reads in 98.203 CPU sec, 3.127 real sec
sorting block 20, #reads = 1070098 mem = 536880801 peak = 990244864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 277570, 4, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 210, 276)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492)
[M::mem_pestat] mean and std.dev: (233.12, 95.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 614)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 347, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (362.48, 172.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 273, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (313.44, 142.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681482 sequences (100000213 bp) in 2.212 CPU sec, 2.367 real sec...
[M::process] read 681240 sequences (100000097 bp) in 1.500 CPU sec, 1.500 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 277694, 9, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 286, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1282)
[M::mem_pestat] mean and std.dev: (265.00, 142.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1666)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 342, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (358.93, 171.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 269, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1158)
[M::mem_pestat] mean and std.dev: (310.52, 210.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1489)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681250 reads in 154.856 CPU sec, 4.936 real sec
[M::mem_process_seqs] Processed 681140 reads in 99.666 CPU sec, 3.182 real sec
sorting block 21, #reads = 1070455 mem = 536878788 peak = 990244864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 277256, 6, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 231, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (273.83, 141.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 959)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 341, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (358.59, 172.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (307, 344, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (348.45, 143.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681270 sequences (100000012 bp) in 2.616 CPU sec, 2.748 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 277698, 11, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (214, 314, 578)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1306)
[M::mem_pestat] mean and std.dev: (413.65, 343.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1787)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 343, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (360.76, 173.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (308, 458, 803)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1793)
[M::mem_pestat] mean and std.dev: (454.11, 250.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2288)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 299, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (332.05, 151.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 681348 sequences (100000068 bp) in 1.430 CPU sec, 1.425 real sec...
[M::mem_process_seqs] Processed 681482 reads in 151.214 CPU sec, 4.810 real sec
[M::mem_process_seqs] Processed 681240 reads in 96.913 CPU sec, 3.074 real sec
sorting block 22, #reads = 1071138 mem = 536881455 peak = 990244864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 278060, 6, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 237, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525)
[M::mem_pestat] mean and std.dev: (230.45, 93.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 643)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 344, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (362.18, 175.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 292, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1253)
[M::mem_pestat] mean and std.dev: (347.58, 252.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1613)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680312 sequences (100000105 bp) in 2.080 CPU sec, 2.225 real sec...
[M::process] read 681300 sequences (100000087 bp) in 1.450 CPU sec, 1.445 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 277349, 9, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 299, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (259.58, 137.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1297)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 344, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (359.26, 171.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 240, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (283.59, 116.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681270 reads in 148.182 CPU sec, 4.718 real sec
sorting block 23, #reads = 1070841 mem = 536879470 peak = 990244864
[M::mem_process_seqs] Processed 681348 reads in 96.213 CPU sec, 3.053 real sec
[M::process] read 679958 sequences (100000077 bp) in 2.474 CPU sec, 2.623 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 277540, 4, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (233, 368, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (355.85, 197.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 344, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (358.08, 168.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 214, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (253.13, 111.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682056 sequences (100000052 bp) in 1.370 CPU sec, 1.370 real sec...
[M::mem_process_seqs] Processed 681300 reads in 85.302 CPU sec, 2.704 real sec
sorting block 24, #reads = 1070252 mem = 536884549 peak = 990244864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 276842, 5, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 240, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589)
[M::mem_pestat] mean and std.dev: (231.13, 99.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 731)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 352, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (364.10, 168.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 353, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (327.24, 94.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 989)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 276903, 2, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 292, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.90, 121.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 359, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (370.19, 171.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 348, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060)
[M::mem_pestat] mean and std.dev: (270.94, 128.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1349)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682466 sequences (100000281 bp) in 1.591 CPU sec, 1.599 real sec...
[M::mem_process_seqs] Processed 680312 reads in 227.342 CPU sec, 7.223 real sec
[M::mem_process_seqs] Processed 679958 reads in 110.273 CPU sec, 3.526 real sec
sorting block 25, #reads = 1071372 mem = 536878713 peak = 1005424640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 277513, 4, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 258, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (261.79, 105.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 343, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (357.04, 169.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 272, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (299.90, 102.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681092 sequences (100000232 bp) in 2.240 CPU sec, 2.430 real sec...
[M::process] read 680896 sequences (100000088 bp) in 1.330 CPU sec, 1.331 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 278288, 7, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 259, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (222.31, 85.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 794)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 341, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (355.94, 169.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 325, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (326.41, 150.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682056 reads in 150.832 CPU sec, 4.803 real sec
sorting block 26, #reads = 1070854 mem = 536879276 peak = 1005424640
[M::mem_process_seqs] Processed 682466 reads in 97.748 CPU sec, 3.108 real sec
sorting block 27, #reads = 1071168 mem = 536877668 peak = 1005424640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 277385, 10, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (223, 258, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (256.92, 88.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 349, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (362.89, 171.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (564, 1290, 2837)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7383)
[M::mem_pestat] mean and std.dev: (1626.60, 1463.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9656)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 246, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696)
[M::mem_pestat] mean and std.dev: (265.51, 137.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 680958 sequences (100000163 bp) in 2.268 CPU sec, 2.437 real sec...
[M::process] read 681334 sequences (100000071 bp) in 1.390 CPU sec, 1.389 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 277606, 2, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 245, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (268.81, 127.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 350, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (363.82, 171.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 385, 694)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1556)
[M::mem_pestat] mean and std.dev: (393.97, 192.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1987)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681092 reads in 153.130 CPU sec, 4.868 real sec
[M::mem_process_seqs] Processed 680896 reads in 98.100 CPU sec, 3.117 real sec
sorting block 28, #reads = 1071059 mem = 536880502 peak = 1005424640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 277129, 10, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (211, 282, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604)
[M::mem_pestat] mean and std.dev: (272.02, 103.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 735)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 352, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (365.86, 172.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (197, 283, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (219.75, 126.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1325)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 298, 683)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1643)
[M::mem_pestat] mean and std.dev: (356.79, 228.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2123)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 681748 sequences (100000219 bp) in 1.965 CPU sec, 2.110 real sec...
[M::process] read 681738 sequences (100000158 bp) in 1.390 CPU sec, 1.383 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 277792, 4, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (223, 355, 897)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2245)
[M::mem_pestat] mean and std.dev: (477.90, 468.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2919)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 344, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (359.51, 170.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 324, 562)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1180)
[M::mem_pestat] mean and std.dev: (376.38, 208.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1489)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680958 reads in 155.130 CPU sec, 4.942 real sec
[M::mem_process_seqs] Processed 681334 reads in 99.415 CPU sec, 3.162 real sec
sorting block 29, #reads = 1069646 mem = 536881080 peak = 1005424640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 277765, 6, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 383, 609)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1441)
[M::mem_pestat] mean and std.dev: (368.92, 226.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1857)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 339, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (355.72, 169.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 275, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (305.94, 140.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681514 sequences (100000039 bp) in 2.266 CPU sec, 2.421 real sec...
[M::process] read 683924 sequences (100000042 bp) in 1.440 CPU sec, 1.444 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 277808, 4, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 353, 793)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1983)
[M::mem_pestat] mean and std.dev: (393.07, 257.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2578)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 340, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (357.59, 171.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 256, 556)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1350)
[M::mem_pestat] mean and std.dev: (345.92, 226.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1747)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681748 reads in 153.563 CPU sec, 4.878 real sec
sorting block 30, #reads = 1070054 mem = 536879347 peak = 1005424640
[M::mem_process_seqs] Processed 681738 reads in 98.308 CPU sec, 3.127 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 277799, 10, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 282, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1201)
[M::mem_pestat] mean and std.dev: (315.18, 196.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1537)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 342, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (360.04, 173.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (879, 1172, 1463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2631)
[M::mem_pestat] mean and std.dev: (1127.20, 580.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3451)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 347, 592)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1378)
[M::mem_pestat] mean and std.dev: (360.57, 194.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1771)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 31, #reads = 1069024 mem = 536878587 peak = 1007931392
[M::process] read 684120 sequences (100000192 bp) in 2.066 CPU sec, 2.210 real sec...
[M::process] read 684454 sequences (100000160 bp) in 1.430 CPU sec, 1.433 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 279314, 9, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 229, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (234.07, 98.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 337, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (357.76, 178.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 262, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568)
[M::mem_pestat] mean and std.dev: (267.55, 86.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 687)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681514 reads in 153.540 CPU sec, 4.887 real sec
[M::mem_process_seqs] Processed 683924 reads in 101.825 CPU sec, 3.235 real sec
sorting block 32, #reads = 1068971 mem = 536882592 peak = 1007931392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 279171, 8, 51)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 310, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1094)
[M::mem_pestat] mean and std.dev: (345.80, 172.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1392)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 337, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (356.08, 176.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 425, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (338.59, 144.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683084 sequences (100000223 bp) in 2.404 CPU sec, 2.546 real sec...
[M::process] read 684516 sequences (100000193 bp) in 1.450 CPU sec, 1.449 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 279570, 3, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 298, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (296.10, 121.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 332, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (351.16, 173.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 253, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 528)
[M::mem_pestat] mean and std.dev: (241.28, 71.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 633)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684120 reads in 155.195 CPU sec, 4.929 real sec
[M::mem_process_seqs] Processed 684454 reads in 100.141 CPU sec, 3.182 real sec
sorting block 33, #reads = 1067342 mem = 536880285 peak = 1007931392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 279307, 5, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (197, 262, 663)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1595)
[M::mem_pestat] mean and std.dev: (354.69, 239.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2061)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 344, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (359.89, 174.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 229, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (291.84, 149.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681610 sequences (100000020 bp) in 2.100 CPU sec, 2.242 real sec...
[M::process] read 682088 sequences (100000142 bp) in 1.430 CPU sec, 1.428 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 280018, 3, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 270, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (305.46, 151.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 331, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (349.92, 171.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 285, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (288.33, 132.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683084 reads in 157.431 CPU sec, 5.018 real sec
[M::mem_process_seqs] Processed 684516 reads in 102.898 CPU sec, 3.277 real sec
sorting block 34, #reads = 1068616 mem = 536882711 peak = 1007931392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 278575, 10, 52)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 262, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (249.54, 118.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 778)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 351, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (365.96, 175.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (63, 63, 1103)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3183)
[M::mem_pestat] mean and std.dev: (584.40, 834.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4223)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 295, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620)
[M::mem_pestat] mean and std.dev: (279.67, 100.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 752)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 681870 sequences (100000073 bp) in 2.248 CPU sec, 2.376 real sec...
[M::process] read 680900 sequences (100000104 bp) in 1.420 CPU sec, 1.423 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278307, 5, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 289, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (303.53, 187.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 346, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (361.54, 173.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 292, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1111)
[M::mem_pestat] mean and std.dev: (311.19, 190.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1416)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681610 reads in 159.681 CPU sec, 5.075 real sec
sorting block 35, #reads = 1068080 mem = 536880302 peak = 1007931392
[M::mem_process_seqs] Processed 682088 reads in 104.083 CPU sec, 3.313 real sec
sorting block 36, #reads = 1066079 mem = 536880802 peak = 1073078272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 278515, 3, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 267, 1048)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2808)
[M::mem_pestat] mean and std.dev: (478.53, 655.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3688)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 341, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (356.99, 169.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 306, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (313.22, 177.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681748 sequences (100000165 bp) in 2.069 CPU sec, 2.194 real sec...
[M::process] read 681400 sequences (100000204 bp) in 1.420 CPU sec, 1.420 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 278268, 3, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 301, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (284.82, 136.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 353, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (366.78, 173.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 272, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (304.81, 165.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681870 reads in 160.408 CPU sec, 5.104 real sec
[M::mem_process_seqs] Processed 680900 reads in 103.590 CPU sec, 3.298 real sec
sorting block 37, #reads = 1065947 mem = 536878886 peak = 1073078272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 278560, 4, 48)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (209, 282, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (269.48, 91.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 343, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (358.91, 171.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 325, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (357.53, 206.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682978 sequences (100000090 bp) in 2.327 CPU sec, 2.461 real sec...
[M::process] read 686828 sequences (100000197 bp) in 1.460 CPU sec, 1.467 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 278328, 3, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 294, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (277.70, 127.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 346, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (363.33, 174.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 261, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (296.31, 178.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681748 reads in 158.622 CPU sec, 5.045 real sec
[M::mem_process_seqs] Processed 681400 reads in 102.288 CPU sec, 3.251 real sec
sorting block 38, #reads = 1065433 mem = 536879156 peak = 1073078272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 279284, 16, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 241, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (249.38, 97.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 341, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (358.58, 175.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (289, 1810, 1829)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4909)
[M::mem_pestat] mean and std.dev: (1425.31, 1099.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6449)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 248, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (262.93, 84.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 685364 sequences (100000094 bp) in 2.168 CPU sec, 2.300 real sec...
[M::process] read 684348 sequences (100000040 bp) in 1.430 CPU sec, 1.429 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 281475, 6, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 266, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565)
[M::mem_pestat] mean and std.dev: (227.43, 75.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 698)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 318, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (336.65, 165.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 275, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614)
[M::mem_pestat] mean and std.dev: (270.84, 106.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 737)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682978 reads in 167.265 CPU sec, 5.328 real sec
sorting block 39, #reads = 1065995 mem = 536878789 peak = 1073078272
[M::mem_process_seqs] Processed 686828 reads in 112.420 CPU sec, 3.579 real sec
sorting block 40, #reads = 1061846 mem = 536879364 peak = 1073078272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 280735, 2, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 225, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778)
[M::mem_pestat] mean and std.dev: (229.85, 113.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 325, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (342.01, 166.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 288, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536)
[M::mem_pestat] mean and std.dev: (258.53, 100.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 660)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685020 sequences (100000005 bp) in 2.372 CPU sec, 2.512 real sec...
[M::process] read 682650 sequences (100000269 bp) in 1.440 CPU sec, 1.432 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 280352, 6, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 246, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623)
[M::mem_pestat] mean and std.dev: (230.44, 86.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 332, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (348.60, 168.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 266, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (267.86, 102.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685364 reads in 163.805 CPU sec, 5.205 real sec
[M::mem_process_seqs] Processed 684348 reads in 104.406 CPU sec, 3.326 real sec
sorting block 41, #reads = 1065040 mem = 536878784 peak = 1073078272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 280268, 3, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (224, 333, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (298.44, 121.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 326, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (343.69, 168.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 331, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1136)
[M::mem_pestat] mean and std.dev: (318.15, 172.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1458)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682522 sequences (100000052 bp) in 2.084 CPU sec, 2.224 real sec...
[M::process] read 682146 sequences (100000067 bp) in 1.410 CPU sec, 1.411 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 280015, 0, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (231, 358, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (308.00, 137.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 345, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (359.90, 173.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 283, 636)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1494)
[M::mem_pestat] mean and std.dev: (370.60, 294.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1923)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685020 reads in 160.912 CPU sec, 5.130 real sec
[M::mem_process_seqs] Processed 682650 reads in 103.669 CPU sec, 3.306 real sec
sorting block 42, #reads = 1065975 mem = 536880535 peak = 1073078272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 279246, 12, 54)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 275, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (316.30, 187.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1302)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 344, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (360.66, 175.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (249, 476, 1106)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2820)
[M::mem_pestat] mean and std.dev: (512.67, 375.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3677)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 297, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1111)
[M::mem_pestat] mean and std.dev: (316.35, 227.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1425)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 680656 sequences (100000101 bp) in 2.236 CPU sec, 2.371 real sec...
[M::process] read 682002 sequences (100000037 bp) in 1.410 CPU sec, 1.415 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 278801, 2, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 237, 599)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1489)
[M::mem_pestat] mean and std.dev: (296.54, 184.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1934)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 347, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (362.64, 175.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 225, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1277)
[M::mem_pestat] mean and std.dev: (314.41, 262.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1652)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682522 reads in 158.994 CPU sec, 5.056 real sec
sorting block 43, #reads = 1066512 mem = 536881011 peak = 1073078272
[M::mem_process_seqs] Processed 682146 reads in 103.055 CPU sec, 3.282 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 278230, 6, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 242, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (272.25, 129.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 356, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (371.41, 177.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1292)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 295, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762)
[M::mem_pestat] mean and std.dev: (294.49, 156.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681914 sequences (100000270 bp) in 2.124 CPU sec, 2.241 real sec...
[M::process] read 683648 sequences (100000185 bp) in 1.440 CPU sec, 1.447 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 278383, 3, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 215, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (252.60, 132.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 344, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (362.33, 176.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 327, 776)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1970)
[M::mem_pestat] mean and std.dev: (463.47, 431.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2567)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680656 reads in 157.249 CPU sec, 5.006 real sec
sorting block 44, #reads = 1064615 mem = 536879445 peak = 1073078272
[M::mem_process_seqs] Processed 682002 reads in 102.214 CPU sec, 3.251 real sec
sorting block 45, #reads = 1065542 mem = 536882489 peak = 1112317952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 278313, 4, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 255, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592)
[M::mem_pestat] mean and std.dev: (268.57, 123.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 764)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 348, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (367.06, 181.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1301)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 258, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055)
[M::mem_pestat] mean and std.dev: (284.00, 137.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1350)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684306 sequences (100000270 bp) in 2.178 CPU sec, 2.303 real sec...
[M::process] read 683320 sequences (100000158 bp) in 1.410 CPU sec, 1.406 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 279691, 5, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (226.55, 137.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 336, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (355.87, 176.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (277, 375, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (369.32, 169.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681914 reads in 164.021 CPU sec, 5.224 real sec
[M::process] read 682266 sequences (100000148 bp) in 1.631 CPU sec, 1.637 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 279787, 1, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 202, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699)
[M::mem_pestat] mean and std.dev: (269.48, 168.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 334, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (350.06, 169.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 304, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1182)
[M::mem_pestat] mean and std.dev: (319.24, 144.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1515)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684306 reads in 85.561 CPU sec, 2.717 real sec
sorting block 46, #reads = 1065370 mem = 536878701 peak = 1112317952
[M::mem_process_seqs] Processed 683648 reads in 217.286 CPU sec, 6.918 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 279244, 4, 58)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (208, 286, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715)
[M::mem_pestat] mean and std.dev: (276.97, 121.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 884)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 341, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (355.55, 171.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 322, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (329.30, 148.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 47, #reads = 1062930 mem = 536879062 peak = 1112317952
[M::process] read 684846 sequences (100000274 bp) in 2.569 CPU sec, 2.717 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 278999, 3, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 176, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762)
[M::mem_pestat] mean and std.dev: (242.34, 156.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 344, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (357.33, 169.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 396, 666)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1502)
[M::mem_pestat] mean and std.dev: (430.58, 249.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1920)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683836 sequences (100000081 bp) in 1.390 CPU sec, 1.394 real sec...
[M::mem_process_seqs] Processed 683320 reads in 168.688 CPU sec, 5.369 real sec
[M::mem_process_seqs] Processed 682266 reads in 99.879 CPU sec, 3.168 real sec
sorting block 48, #reads = 1069263 mem = 536877311 peak = 1112317952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 279764, 2, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 260, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503)
[M::mem_pestat] mean and std.dev: (216.55, 80.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 614)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 325, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (341.44, 164.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 308, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1133)
[M::mem_pestat] mean and std.dev: (320.91, 142.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1446)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682080 sequences (100000129 bp) in 2.547 CPU sec, 2.721 real sec...
[M::process] read 683552 sequences (100000228 bp) in 1.390 CPU sec, 1.387 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 279674, 15, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 263, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1224)
[M::mem_pestat] mean and std.dev: (339.20, 254.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1576)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 332, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (347.88, 167.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (875, 1136, 1274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (77, 2072)
[M::mem_pestat] mean and std.dev: (1038.43, 255.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2471)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 333, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (286.97, 113.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684846 reads in 156.372 CPU sec, 4.969 real sec
[M::mem_process_seqs] Processed 683836 reads in 99.962 CPU sec, 3.174 real sec
sorting block 49, #reads = 1068331 mem = 536880397 peak = 1112317952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 278975, 2, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 303, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (279.70, 116.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 981)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 346, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (359.89, 170.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 345, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (258.81, 110.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682822 sequences (100000072 bp) in 2.311 CPU sec, 2.435 real sec...
[M::process] read 682692 sequences (100000143 bp) in 1.440 CPU sec, 1.443 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 279118, 11, 56)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 282, 870)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2314)
[M::mem_pestat] mean and std.dev: (508.22, 593.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3036)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 334, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (349.92, 168.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1289, 1683, 1725)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (417, 2597)
[M::mem_pestat] mean and std.dev: (1429.25, 405.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3050)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 231, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (259.66, 106.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682080 reads in 156.208 CPU sec, 4.968 real sec
sorting block 50, #reads = 1068586 mem = 536879654 peak = 1112317952
[M::mem_process_seqs] Processed 683552 reads in 100.669 CPU sec, 3.197 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 279213, 1, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (121, 270, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (257.09, 136.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 340, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (355.25, 170.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 255, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (267.88, 137.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682572 sequences (100000242 bp) in 2.374 CPU sec, 2.508 real sec...
[M::process] read 683412 sequences (100000090 bp) in 1.420 CPU sec, 1.428 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 278451, 10, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 265, 1165)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3133)
[M::mem_pestat] mean and std.dev: (600.81, 657.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4117)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 340, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (357.05, 172.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 80, 578)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1666)
[M::mem_pestat] mean and std.dev: (353.30, 448.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2210)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 248, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (273.90, 116.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1061)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682822 reads in 160.151 CPU sec, 5.093 real sec
sorting block 51, #reads = 1068323 mem = 536880090 peak = 1112317952
[M::mem_process_seqs] Processed 682692 reads in 104.567 CPU sec, 3.326 real sec
sorting block 52, #reads = 1066757 mem = 536878712 peak = 1112317952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 278405, 11, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 296, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (273.67, 125.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 343, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (359.39, 174.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (650, 650, 1597)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3491)
[M::mem_pestat] mean and std.dev: (838.90, 586.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4438)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 339, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (319.25, 146.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 686624 sequences (100000178 bp) in 2.406 CPU sec, 2.537 real sec...
[M::process] read 687056 sequences (100000210 bp) in 1.470 CPU sec, 1.470 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 279540, 10, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 338, 589)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1413)
[M::mem_pestat] mean and std.dev: (350.86, 188.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1825)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 333, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (353.98, 175.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (77, 619, 1292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3722)
[M::mem_pestat] mean and std.dev: (598.67, 771.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4937)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 287, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1126)
[M::mem_pestat] mean and std.dev: (334.00, 183.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1435)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682572 reads in 164.648 CPU sec, 5.233 real sec
[M::mem_process_seqs] Processed 683412 reads in 108.390 CPU sec, 3.449 real sec
sorting block 53, #reads = 1065338 mem = 536877422 peak = 1112317952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 279825, 12, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 298, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (322.00, 174.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 321, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (337.60, 164.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (267, 720, 1605)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4281)
[M::mem_pestat] mean and std.dev: (866.00, 766.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5619)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 239, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (274.27, 128.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 685242 sequences (100000030 bp) in 2.502 CPU sec, 2.636 real sec...
[M::process] read 687508 sequences (100000243 bp) in 1.430 CPU sec, 1.431 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 280695, 4, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 236, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (243.71, 128.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 316, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (331.32, 159.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 315, 768)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1964)
[M::mem_pestat] mean and std.dev: (404.53, 297.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2562)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686624 reads in 178.749 CPU sec, 5.685 real sec
[M::mem_process_seqs] Processed 687056 reads in 114.647 CPU sec, 3.653 real sec
sorting block 54, #reads = 1059902 mem = 536881208 peak = 1112317952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 280237, 3, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 218, 630)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1532)
[M::mem_pestat] mean and std.dev: (393.96, 309.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1983)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 331, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (343.94, 164.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 392, 629)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1459)
[M::mem_pestat] mean and std.dev: (435.27, 307.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1874)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685342 sequences (100000156 bp) in 2.462 CPU sec, 2.578 real sec...
[M::process] read 683978 sequences (100000035 bp) in 1.450 CPU sec, 1.446 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 280596, 8, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 261, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (247.50, 110.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 311, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (328.34, 159.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 326, 537)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1213)
[M::mem_pestat] mean and std.dev: (366.53, 229.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1551)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685242 reads in 168.499 CPU sec, 5.359 real sec
sorting block 55, #reads = 1060141 mem = 536878419 peak = 1112317952
[M::mem_process_seqs] Processed 687508 reads in 109.288 CPU sec, 3.473 real sec
sorting block 56, #reads = 1064675 mem = 536883501 peak = 1112461312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 280326, 10, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 203, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (292.62, 182.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 321, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (336.12, 160.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (43, 144, 210)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544)
[M::mem_pestat] mean and std.dev: (135.56, 100.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 711)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 252, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (271.57, 140.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 684008 sequences (100000053 bp) in 2.353 CPU sec, 2.483 real sec...
[M::process] read 683780 sequences (100000224 bp) in 1.410 CPU sec, 1.409 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 279830, 13, 56)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 209, 582)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1482)
[M::mem_pestat] mean and std.dev: (305.05, 257.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1932)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 330, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (343.87, 162.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (889, 889, 1304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (59, 2134)
[M::mem_pestat] mean and std.dev: (1130.80, 320.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2549)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 340, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1153)
[M::mem_pestat] mean and std.dev: (373.38, 232.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1471)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685342 reads in 163.626 CPU sec, 5.206 real sec
[M::mem_process_seqs] Processed 683978 reads in 105.132 CPU sec, 3.346 real sec
sorting block 57, #reads = 1063898 mem = 536881311 peak = 1112461312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 279130, 6, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 239, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504)
[M::mem_pestat] mean and std.dev: (222.68, 93.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 625)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 332, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (344.99, 163.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 254, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (286.08, 108.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683152 sequences (100000083 bp) in 2.457 CPU sec, 2.573 real sec...
[M::process] read 682886 sequences (100000235 bp) in 1.430 CPU sec, 1.434 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 278779, 3, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 208, 241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 379)
[M::mem_pestat] mean and std.dev: (191.10, 58.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 448)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 332, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (346.69, 165.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 223, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (259.96, 100.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 893)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684008 reads in 163.551 CPU sec, 5.202 real sec
[M::mem_process_seqs] Processed 683780 reads in 105.688 CPU sec, 3.364 real sec
sorting block 58, #reads = 1066029 mem = 536876947 peak = 1112461312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 278492, 10, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (246, 390, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (354.92, 159.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 333, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (349.13, 166.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (955, 1131, 2205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4705)
[M::mem_pestat] mean and std.dev: (1131.11, 713.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5955)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 304, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (308.21, 155.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 497278 sequences (72800609 bp) in 2.114 CPU sec, 2.235 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278293, 9, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (310, 361, 572)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1096)
[M::mem_pestat] mean and std.dev: (392.47, 158.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1358)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 336, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (353.28, 170.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 329, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074)
[M::mem_pestat] mean and std.dev: (345.81, 182.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1367)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683152 reads in 159.259 CPU sec, 5.123 real sec
sorting block 59, #reads = 1065470 mem = 536881284 peak = 1112461312
[M::mem_process_seqs] Processed 682886 reads in 101.560 CPU sec, 3.283 real sec
sorting block 60, #reads = 1066331 mem = 536877834 peak = 1204670464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 203205, 2, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 262, 534)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1260)
[M::mem_pestat] mean and std.dev: (385.61, 344.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1765)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 336, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (353.94, 172.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 257, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (248.38, 82.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 497278 reads in 64.141 CPU sec, 2.141 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 65310316
overall: 70050910208 mem 9561.098 user 320.975 sys 684.972 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1029_N5D-T1-TDNA-1_A23MHWWLT4_1.L008\tPU:L008\tSM:1029_N5D-T1-TDNA-1_A23MHWWLT4_1\tLB:1029_N5D-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0003.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0003.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 685.012 sec; CPU: 9882.076 sec
sorting block 61, #reads = 533203 mem = 268340638 peak = 1204670464
uniq header count 1
merging 62 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3973748110
output reads: 65693821
bam_mem_sort: 62 calls 22.262 user 0.240 sys 22.682 real
bam_write: 62 calls 46.954 user 1.172 sys 48.560 real
execute: 1 calls 12.422 user 0.547 sys 707.302 real
merge_files: 1 calls 12.412 user 0.519 sys 21.536 real
parse_chunk: 984 calls 30.375 user 8.403 sys 39.588 real
read_chunk: 2658 calls 24.667 user 24.552 sys 270.811 real
sort_block: 1 calls 19.440 user 23.996 sys 263.017 real
write_chunk: 1672 calls 12.112 user 0.356 sys 20.840 real
overall: 2895212544 mem 807.414 user 44.094 sys 707.322 real
ls: cannot access '*.cram': No such file or directory