cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_Kh-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1887.590 CPU sec, 76.033 real sec
[M::process] read 672796 sequences (100000246 bp) in 0.999 CPU sec, 1.010 real sec...
[M::process] read 672102 sequences (100000108 bp) in 1.238 CPU sec, 1.238 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278946, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 355, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (373.67, 161.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671698 sequences (100000129 bp) in 1.257 CPU sec, 1.258 real sec...
[M::process] read 671466 sequences (100000059 bp) in 1.229 CPU sec, 1.233 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278557, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 364, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (381.94, 164.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672796 reads in 143.119 CPU sec, 4.506 real sec
[M::mem_process_seqs] Processed 672102 reads in 85.851 CPU sec, 2.730 real sec
sorting block 0, #reads = 1076082 mem = 536879400 peak = 602652672
[M::process] read 671348 sequences (100000237 bp) in 1.498 CPU sec, 1.719 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278250, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 370, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (386.02, 165.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671210 sequences (100000064 bp) in 1.236 CPU sec, 1.242 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278321, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 373, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (390.19, 167.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278312, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 378, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (393.59, 168.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (1332, 1498, 5630)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14226)
[M::mem_pestat] mean and std.dev: (2335.55, 2059.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 18524)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 671698 reads in 201.237 CPU sec, 6.429 real sec
[M::mem_process_seqs] Processed 671466 reads in 147.602 CPU sec, 4.728 real sec
[M::mem_process_seqs] Processed 671348 reads in 159.450 CPU sec, 5.125 real sec
sorting block 1, #reads = 1075399 mem = 536878048 peak = 730054656
[M::process] read 670954 sequences (100000217 bp) in 1.953 CPU sec, 2.160 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278240, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 380, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (395.42, 169.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 2, #reads = 1076068 mem = 536880942 peak = 759816192
[M::process] read 671006 sequences (100000146 bp) in 1.750 CPU sec, 1.845 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277673, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 383, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (398.20, 170.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671210 reads in 147.367 CPU sec, 4.708 real sec
[M::mem_process_seqs] Processed 670954 reads in 90.324 CPU sec, 2.849 real sec
[M::process] read 670632 sequences (100000035 bp) in 1.836 CPU sec, 1.932 real sec...
sorting block 3, #reads = 1075852 mem = 536879974 peak = 759816192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278344, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 384, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (398.96, 169.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670754 sequences (100000097 bp) in 1.894 CPU sec, 2.014 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278267, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 386, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (401.54, 170.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670716 sequences (100000056 bp) in 1.413 CPU sec, 1.426 real sec...
[M::mem_process_seqs] Processed 671006 reads in 144.398 CPU sec, 4.586 real sec
[M::mem_process_seqs] Processed 670632 reads in 88.722 CPU sec, 2.803 real sec
sorting block 4, #reads = 1074731 mem = 536879152 peak = 802807808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277565, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 385, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (401.38, 171.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670756 sequences (100000111 bp) in 2.008 CPU sec, 2.170 real sec...
[M::process] read 670886 sequences (100000071 bp) in 1.528 CPU sec, 1.525 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 277324, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 384, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (399.80, 170.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670754 reads in 144.329 CPU sec, 4.582 real sec
[M::mem_process_seqs] Processed 670716 reads in 88.021 CPU sec, 2.787 real sec
sorting block 5, #reads = 1076377 mem = 536879108 peak = 802807808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 277885, 1, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (381, 5619, 5619)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16095)
[M::mem_pestat] mean and std.dev: (3000.00, 2619.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 21333)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 384, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (400.51, 171.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 671092 sequences (100000266 bp) in 2.623 CPU sec, 2.782 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277599, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 385, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (399.95, 170.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670756 reads in 146.344 CPU sec, 4.626 real sec
sorting block 6, #reads = 1075235 mem = 536878582 peak = 802807808
[M::mem_process_seqs] Processed 670886 reads in 89.911 CPU sec, 2.819 real sec
[M::process] read 670920 sequences (100000177 bp) in 1.993 CPU sec, 2.030 real sec...
sorting block 7, #reads = 1075133 mem = 536877542 peak = 883728384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277484, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 379, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (395.63, 169.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670916 sequences (100000145 bp) in 2.037 CPU sec, 2.187 real sec...
[M::process] read 670776 sequences (100000247 bp) in 1.455 CPU sec, 1.459 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277703, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 377, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (394.09, 168.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671092 reads in 148.294 CPU sec, 4.721 real sec
[M::mem_process_seqs] Processed 670920 reads in 92.419 CPU sec, 2.929 real sec
sorting block 8, #reads = 1075751 mem = 536875698 peak = 883728384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277041, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 378, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (394.58, 168.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670712 sequences (100000112 bp) in 2.445 CPU sec, 2.595 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277549, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 382, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (397.55, 169.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670822 sequences (100000108 bp) in 1.519 CPU sec, 1.525 real sec...
[M::mem_process_seqs] Processed 670916 reads in 149.568 CPU sec, 4.750 real sec
[M::mem_process_seqs] Processed 670776 reads in 91.949 CPU sec, 2.907 real sec
sorting block 9, #reads = 1075587 mem = 536881989 peak = 883728384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277335, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 380, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (397.52, 169.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670600 sequences (100000075 bp) in 2.227 CPU sec, 2.393 real sec...
[M::process] read 670166 sequences (100000000 bp) in 1.440 CPU sec, 1.443 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277222, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 380, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (396.58, 169.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670712 reads in 151.637 CPU sec, 4.829 real sec
[M::mem_process_seqs] Processed 670822 reads in 91.846 CPU sec, 2.906 real sec
sorting block 10, #reads = 1075172 mem = 536878043 peak = 883728384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 276988, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 380, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (396.43, 168.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670432 sequences (100000042 bp) in 2.831 CPU sec, 3.031 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277019, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 386, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (401.89, 169.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670600 reads in 150.496 CPU sec, 4.776 real sec
sorting block 11, #reads = 1075419 mem = 536879577 peak = 883728384
[M::mem_process_seqs] Processed 670166 reads in 92.524 CPU sec, 2.909 real sec
[M::process] read 670384 sequences (100000028 bp) in 2.078 CPU sec, 2.139 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 276935, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 382, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (397.92, 168.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 12, #reads = 1075106 mem = 536879141 peak = 883728384
[M::process] read 670304 sequences (100000135 bp) in 2.335 CPU sec, 2.511 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277317, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 380, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (396.66, 167.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670432 reads in 151.309 CPU sec, 4.836 real sec
[M::mem_process_seqs] Processed 670384 reads in 92.841 CPU sec, 2.925 real sec
[M::process] read 670382 sequences (100000003 bp) in 1.695 CPU sec, 1.746 real sec...
sorting block 13, #reads = 1075271 mem = 536880281 peak = 883728384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277131, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 379, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (394.93, 165.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670202 sequences (100000182 bp) in 2.610 CPU sec, 2.794 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277449, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 379, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (396.05, 167.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670304 reads in 148.617 CPU sec, 4.733 real sec
[M::mem_process_seqs] Processed 670382 reads in 90.424 CPU sec, 2.852 real sec
[M::process] read 670140 sequences (100000207 bp) in 1.705 CPU sec, 1.739 real sec...
sorting block 14, #reads = 1076177 mem = 536878165 peak = 883728384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277581, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 383, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (400.17, 169.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 669966 sequences (100000018 bp) in 2.441 CPU sec, 2.631 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278196, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 386, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (402.61, 170.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670202 reads in 145.887 CPU sec, 4.656 real sec
[M::mem_process_seqs] Processed 670140 reads in 87.893 CPU sec, 2.774 real sec
[M::process] read 670024 sequences (100000223 bp) in 2.066 CPU sec, 2.162 real sec...
sorting block 15, #reads = 1076482 mem = 536878862 peak = 883728384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278331, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 390, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (407.06, 173.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671992 sequences (100000101 bp) in 2.486 CPU sec, 2.652 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278038, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 388, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (405.59, 172.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 669966 reads in 141.531 CPU sec, 4.520 real sec
sorting block 16, #reads = 1076854 mem = 536875817 peak = 883728384
[M::mem_process_seqs] Processed 670024 reads in 88.018 CPU sec, 2.779 real sec
[M::process] read 671770 sequences (100000249 bp) in 2.395 CPU sec, 2.542 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278408, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 367, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (385.03, 167.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 17, #reads = 1076145 mem = 536878074 peak = 883728384
[M::process] read 671686 sequences (100000037 bp) in 2.362 CPU sec, 2.546 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 277143, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 372, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (389.13, 169.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671992 reads in 148.720 CPU sec, 4.760 real sec
[M::mem_process_seqs] Processed 671770 reads in 92.040 CPU sec, 2.911 real sec
[M::process] read 671374 sequences (100000150 bp) in 2.198 CPU sec, 2.343 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277899, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 376, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (392.88, 170.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 18, #reads = 1075961 mem = 536881206 peak = 883728384
[M::process] read 671248 sequences (100000099 bp) in 2.551 CPU sec, 2.735 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277940, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 379, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (395.37, 170.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671686 reads in 146.517 CPU sec, 4.684 real sec
[M::mem_process_seqs] Processed 671374 reads in 90.430 CPU sec, 2.859 real sec
sorting block 19, #reads = 1077036 mem = 536879228 peak = 883728384
[M::process] read 670974 sequences (100000182 bp) in 2.231 CPU sec, 2.404 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277638, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 380, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (397.28, 171.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671072 sequences (100000285 bp) in 2.253 CPU sec, 2.427 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277470, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 384, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (399.58, 171.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671248 reads in 147.650 CPU sec, 4.728 real sec
[M::mem_process_seqs] Processed 670974 reads in 92.183 CPU sec, 2.913 real sec
sorting block 20, #reads = 1077620 mem = 536880094 peak = 883728384
[M::process] read 670766 sequences (100000226 bp) in 2.273 CPU sec, 2.446 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277585, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 385, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (400.81, 172.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670900 sequences (100000226 bp) in 2.407 CPU sec, 2.607 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 277640, 6, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (475, 475, 955)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1915)
[M::mem_pestat] mean and std.dev: (642.00, 265.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2395)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 386, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (401.08, 171.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 671072 reads in 151.056 CPU sec, 4.836 real sec
sorting block 21, #reads = 1076264 mem = 536880114 peak = 883728384
[M::mem_process_seqs] Processed 670766 reads in 92.835 CPU sec, 2.936 real sec
[M::process] read 670592 sequences (100000122 bp) in 2.360 CPU sec, 2.508 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 277873, 0, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (340, 340, 561)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (350.55, 119.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 388, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (403.66, 173.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 22, #reads = 1077119 mem = 536878145 peak = 883728384
[M::process] read 670390 sequences (100000278 bp) in 2.042 CPU sec, 2.180 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277876, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 389, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (404.20, 172.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670900 reads in 150.194 CPU sec, 4.805 real sec
[M::mem_process_seqs] Processed 670592 reads in 91.759 CPU sec, 2.897 real sec
[M::process] read 670550 sequences (100000233 bp) in 1.807 CPU sec, 1.868 real sec...
sorting block 23, #reads = 1075523 mem = 536882279 peak = 883728384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277574, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 391, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (406.17, 173.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670666 sequences (100000036 bp) in 2.427 CPU sec, 2.599 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278042, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 391, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (406.70, 174.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670390 reads in 151.868 CPU sec, 4.851 real sec
[M::process] read 670872 sequences (100000199 bp) in 1.490 CPU sec, 1.495 real sec...
sorting block 24, #reads = 1076889 mem = 536878742 peak = 883728384
[M::process] read 670840 sequences (100000253 bp) in 1.557 CPU sec, 1.567 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277778, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 390, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (405.78, 174.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670666 reads in 88.062 CPU sec, 3.004 real sec
[M::mem_process_seqs] Processed 670550 reads in 207.631 CPU sec, 6.811 real sec
sorting block 25, #reads = 1076025 mem = 536879552 peak = 883728384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 277332, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 383, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (399.61, 171.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670990 sequences (100000003 bp) in 2.443 CPU sec, 2.576 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277877, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 383, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (401.14, 173.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670872 reads in 164.327 CPU sec, 5.231 real sec
[M::mem_process_seqs] Processed 670840 reads in 95.615 CPU sec, 3.014 real sec
[M::process] read 670930 sequences (100000261 bp) in 2.272 CPU sec, 2.374 real sec...
sorting block 26, #reads = 1075686 mem = 536877979 peak = 883728384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277697, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 384, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (400.65, 173.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670754 sequences (100000212 bp) in 2.152 CPU sec, 2.334 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277616, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 383, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (399.54, 172.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670854 sequences (100000138 bp) in 1.450 CPU sec, 1.449 real sec...
[M::mem_process_seqs] Processed 670990 reads in 153.648 CPU sec, 4.901 real sec
[M::mem_process_seqs] Processed 670930 reads in 92.000 CPU sec, 2.915 real sec
sorting block 27, #reads = 1075035 mem = 536881221 peak = 883728384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277625, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 383, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (399.07, 171.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670706 sequences (100000096 bp) in 2.400 CPU sec, 2.566 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277553, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 381, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (398.66, 171.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670652 sequences (100000171 bp) in 1.480 CPU sec, 1.495 real sec...
[M::mem_process_seqs] Processed 670754 reads in 147.324 CPU sec, 4.689 real sec
sorting block 28, #reads = 1076080 mem = 536878275 peak = 883728384
[M::mem_process_seqs] Processed 670854 reads in 89.105 CPU sec, 2.822 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278219, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 382, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (399.75, 172.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 29, #reads = 1075736 mem = 536875109 peak = 962871296
[M::process] read 670676 sequences (100000034 bp) in 2.649 CPU sec, 2.796 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278026, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 380, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (397.75, 170.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670796 sequences (100000093 bp) in 1.725 CPU sec, 1.732 real sec...
[M::mem_process_seqs] Processed 670706 reads in 163.676 CPU sec, 5.202 real sec
[M::mem_process_seqs] Processed 670652 reads in 104.674 CPU sec, 3.312 real sec
sorting block 30, #reads = 1075405 mem = 536877896 peak = 962871296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277730, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 387, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (402.77, 173.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1280)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670442 sequences (100000191 bp) in 2.923 CPU sec, 3.097 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277842, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 386, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (402.39, 172.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670498 sequences (100000196 bp) in 1.693 CPU sec, 1.697 real sec...
[M::mem_process_seqs] Processed 670676 reads in 165.043 CPU sec, 5.239 real sec
[M::mem_process_seqs] Processed 670796 reads in 104.920 CPU sec, 3.313 real sec
sorting block 31, #reads = 1075313 mem = 536879938 peak = 962871296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 278048, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 387, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (404.45, 173.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670372 sequences (100000122 bp) in 3.025 CPU sec, 3.210 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 278272, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 383, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (400.66, 172.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670442 reads in 165.544 CPU sec, 5.251 real sec
[M::process] read 670288 sequences (100000010 bp) in 1.759 CPU sec, 1.762 real sec...
[M::mem_process_seqs] Processed 670498 reads in 105.793 CPU sec, 3.339 real sec
sorting block 32, #reads = 1075045 mem = 536881558 peak = 962871296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 277540, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 385, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (401.88, 171.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670072 sequences (100000225 bp) in 2.762 CPU sec, 2.961 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277868, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 387, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (403.55, 172.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 669906 sequences (100000022 bp) in 1.630 CPU sec, 1.632 real sec...
[M::mem_process_seqs] Processed 670372 reads in 164.562 CPU sec, 5.237 real sec
sorting block 33, #reads = 1075989 mem = 536879383 peak = 962871296
[M::mem_process_seqs] Processed 670288 reads in 105.513 CPU sec, 3.344 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278285, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 391, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (408.34, 174.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 34, #reads = 1074501 mem = 536875859 peak = 962871296
[M::process] read 671654 sequences (100000035 bp) in 2.865 CPU sec, 3.054 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277564, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (277, 397, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045)
[M::mem_pestat] mean and std.dev: (413.69, 177.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1301)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670072 reads in 160.184 CPU sec, 5.082 real sec
[M::process] read 672176 sequences (100000049 bp) in 1.880 CPU sec, 1.889 real sec...
[M::mem_process_seqs] Processed 669906 reads in 101.616 CPU sec, 3.202 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278335, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 369, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (387.56, 168.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 35, #reads = 1075037 mem = 536877669 peak = 962871296
[M::process] read 671782 sequences (100000239 bp) in 2.997 CPU sec, 3.180 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278739, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 365, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (381.95, 164.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671654 reads in 168.105 CPU sec, 5.369 real sec
[M::process] read 671562 sequences (100000160 bp) in 2.012 CPU sec, 2.056 real sec...
[M::mem_process_seqs] Processed 672176 reads in 108.712 CPU sec, 3.439 real sec
sorting block 36, #reads = 1074910 mem = 536878920 peak = 962871296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278112, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 367, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (383.69, 163.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671354 sequences (100000094 bp) in 3.148 CPU sec, 3.361 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278195, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 373, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (388.21, 164.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671782 reads in 171.785 CPU sec, 5.485 real sec
[M::mem_process_seqs] Processed 671562 reads in 108.726 CPU sec, 3.433 real sec
[M::process] read 671116 sequences (100000291 bp) in 2.442 CPU sec, 2.539 real sec...
sorting block 37, #reads = 1075177 mem = 536879019 peak = 962871296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278155, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 375, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (390.18, 165.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (2080, 2080, 5617)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12691)
[M::mem_pestat] mean and std.dev: (2840.70, 1989.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16228)
[M::mem_pestat] skip orientation RR
[M::process] read 671032 sequences (100000042 bp) in 2.470 CPU sec, 2.655 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 277986, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 375, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (389.98, 164.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671354 reads in 163.992 CPU sec, 5.234 real sec
[M::process] read 671026 sequences (100000102 bp) in 1.543 CPU sec, 1.544 real sec...
sorting block 38, #reads = 1076518 mem = 536879053 peak = 962871296
[M::mem_process_seqs] Processed 671116 reads in 101.045 CPU sec, 3.185 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278019, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 376, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (390.63, 164.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 39, #reads = 1075022 mem = 536879930 peak = 962871296
[M::process] read 670928 sequences (100000220 bp) in 2.617 CPU sec, 2.783 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 278663, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 379, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (392.82, 165.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670702 sequences (100000047 bp) in 1.550 CPU sec, 1.541 real sec...
[M::mem_process_seqs] Processed 671032 reads in 152.909 CPU sec, 4.861 real sec
[M::mem_process_seqs] Processed 671026 reads in 95.210 CPU sec, 3.015 real sec
sorting block 40, #reads = 1075325 mem = 536886819 peak = 962871296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277608, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 382, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (396.09, 166.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670448 sequences (100000174 bp) in 2.613 CPU sec, 2.780 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277132, 1, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 383, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (397.77, 167.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (456, 456, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (456, 456)
[M::mem_pestat] mean and std.dev: (456.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (456, 456)
[M::mem_pestat] skip orientation RR
[M::process] read 670614 sequences (100000071 bp) in 1.570 CPU sec, 1.578 real sec...
[M::mem_process_seqs] Processed 670928 reads in 161.845 CPU sec, 5.141 real sec
[M::mem_process_seqs] Processed 670702 reads in 101.366 CPU sec, 3.203 real sec
sorting block 41, #reads = 1075744 mem = 536879355 peak = 962871296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277129, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 388, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (401.59, 168.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670522 sequences (100000116 bp) in 3.102 CPU sec, 3.285 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277448, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 385, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (398.99, 167.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670448 reads in 161.156 CPU sec, 5.114 real sec
[M::mem_process_seqs] Processed 670614 reads in 101.465 CPU sec, 3.190 real sec
[M::process] read 670600 sequences (100000206 bp) in 2.084 CPU sec, 2.160 real sec...
sorting block 42, #reads = 1075524 mem = 536880496 peak = 962871296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277112, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 387, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (401.36, 169.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670732 sequences (100000102 bp) in 2.383 CPU sec, 2.544 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 276977, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 385, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (399.40, 168.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670800 sequences (100000263 bp) in 1.667 CPU sec, 1.669 real sec...
[M::mem_process_seqs] Processed 670522 reads in 165.987 CPU sec, 5.277 real sec
sorting block 43, #reads = 1074999 mem = 536879189 peak = 962871296
[M::mem_process_seqs] Processed 670600 reads in 102.025 CPU sec, 3.223 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277903, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 383, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (397.77, 168.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 44, #reads = 1074243 mem = 536879520 peak = 979116032
[M::process] read 670660 sequences (100000280 bp) in 2.934 CPU sec, 3.115 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277834, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 382, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (397.55, 169.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670732 reads in 160.625 CPU sec, 5.096 real sec
[M::process] read 670846 sequences (100000140 bp) in 1.822 CPU sec, 1.859 real sec...
[M::mem_process_seqs] Processed 670800 reads in 100.545 CPU sec, 3.168 real sec
sorting block 45, #reads = 1074502 mem = 536879713 peak = 979116032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277868, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 382, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (398.06, 169.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670688 sequences (100000063 bp) in 2.932 CPU sec, 3.124 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277001, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 381, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (397.26, 169.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670666 sequences (100000219 bp) in 1.886 CPU sec, 1.889 real sec...
[M::mem_process_seqs] Processed 670660 reads in 177.802 CPU sec, 5.676 real sec
[M::mem_process_seqs] Processed 670846 reads in 114.120 CPU sec, 3.634 real sec
sorting block 46, #reads = 1075276 mem = 536880709 peak = 979116032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277123, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 378, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (395.56, 168.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670532 sequences (100000019 bp) in 2.581 CPU sec, 2.855 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277299, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 383, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (398.91, 169.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670552 sequences (100000093 bp) in 1.855 CPU sec, 1.894 real sec...
[M::mem_process_seqs] Processed 670688 reads in 185.592 CPU sec, 5.977 real sec
[M::mem_process_seqs] Processed 670666 reads in 116.015 CPU sec, 3.734 real sec
sorting block 47, #reads = 1075144 mem = 536880527 peak = 979116032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 276800, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 383, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (400.21, 171.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670460 sequences (100000064 bp) in 3.032 CPU sec, 3.367 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277336, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 384, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (400.48, 171.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670510 sequences (100000231 bp) in 1.810 CPU sec, 1.838 real sec...
[M::mem_process_seqs] Processed 670532 reads in 185.804 CPU sec, 6.000 real sec
sorting block 48, #reads = 1076607 mem = 536880504 peak = 979116032
[M::mem_process_seqs] Processed 670552 reads in 115.949 CPU sec, 3.853 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277042, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 385, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (401.34, 171.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 49, #reads = 1075368 mem = 536880989 peak = 1006473216
[M::process] read 670290 sequences (100000014 bp) in 2.705 CPU sec, 3.110 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277063, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 386, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (401.77, 171.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670460 reads in 185.871 CPU sec, 6.062 real sec
[M::process] read 670318 sequences (100000281 bp) in 1.724 CPU sec, 1.765 real sec...
[M::mem_process_seqs] Processed 670510 reads in 116.938 CPU sec, 3.767 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 276931, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 389, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (405.33, 172.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 50, #reads = 1074680 mem = 536878746 peak = 1006473216
[M::process] read 670306 sequences (100000233 bp) in 2.530 CPU sec, 2.798 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277166, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 388, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (405.32, 173.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670290 reads in 177.413 CPU sec, 5.751 real sec
[M::mem_process_seqs] Processed 670318 reads in 111.968 CPU sec, 3.596 real sec
[M::process] read 669970 sequences (100000054 bp) in 2.109 CPU sec, 2.251 real sec...
sorting block 51, #reads = 1075760 mem = 536878904 peak = 1006473216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277281, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 391, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (407.70, 174.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 669998 sequences (100000249 bp) in 2.440 CPU sec, 2.870 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 277687, 1, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (450, 450, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (342, 612)
[M::mem_pestat] mean and std.dev: (466.20, 24.75)
[M::mem_pestat] low and high boundaries for proper pairs: (288, 666)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 389, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (405.78, 172.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 670306 reads in 171.609 CPU sec, 5.545 real sec
[M::mem_process_seqs] Processed 669970 reads in 108.363 CPU sec, 3.446 real sec
[M::process] read 671892 sequences (100000291 bp) in 2.283 CPU sec, 2.401 real sec...
sorting block 52, #reads = 1075538 mem = 536880366 peak = 1006473216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 278151, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (275, 393, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (409.00, 174.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671848 sequences (100000167 bp) in 2.541 CPU sec, 2.878 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277381, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 368, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (387.87, 169.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 669998 reads in 173.958 CPU sec, 5.621 real sec
sorting block 53, #reads = 1075262 mem = 536877960 peak = 1006473216
[M::mem_process_seqs] Processed 671892 reads in 114.935 CPU sec, 3.665 real sec
[M::process] read 671852 sequences (100000171 bp) in 2.841 CPU sec, 3.017 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277176, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 371, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (389.76, 171.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 54, #reads = 1075060 mem = 536879411 peak = 1006473216
[M::process] read 671482 sequences (100000294 bp) in 2.546 CPU sec, 2.729 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 276991, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 375, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (392.75, 171.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671848 reads in 185.859 CPU sec, 6.015 real sec
[M::mem_process_seqs] Processed 671852 reads in 119.548 CPU sec, 3.803 real sec
[M::process] read 671456 sequences (100000286 bp) in 2.381 CPU sec, 2.607 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277167, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 379, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (395.68, 171.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 55, #reads = 1076320 mem = 536879501 peak = 1006473216
[M::process] read 671260 sequences (100000263 bp) in 2.349 CPU sec, 2.670 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277308, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 380, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (396.12, 171.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671482 reads in 191.871 CPU sec, 6.203 real sec
[M::mem_process_seqs] Processed 671456 reads in 123.335 CPU sec, 3.955 real sec
[M::process] read 671132 sequences (100000079 bp) in 2.223 CPU sec, 2.398 real sec...
sorting block 56, #reads = 1076166 mem = 536878373 peak = 1006473216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277708, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 381, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (397.62, 172.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671054 sequences (100000021 bp) in 2.283 CPU sec, 2.517 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 277478, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 384, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (399.48, 171.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671260 reads in 183.530 CPU sec, 5.931 real sec
[M::process] read 670810 sequences (100000054 bp) in 2.125 CPU sec, 2.198 real sec...
[M::mem_process_seqs] Processed 671132 reads in 117.567 CPU sec, 3.750 real sec
sorting block 57, #reads = 1076262 mem = 536877281 peak = 1006473216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 277306, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 384, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (399.35, 170.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671484 sequences (100000291 bp) in 2.787 CPU sec, 3.232 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277061, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 384, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (398.52, 169.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671054 reads in 184.973 CPU sec, 5.973 real sec
[M::process] read 670666 sequences (100000020 bp) in 1.923 CPU sec, 1.934 real sec...
sorting block 58, #reads = 1076591 mem = 536879817 peak = 1006473216
[M::mem_process_seqs] Processed 670810 reads in 115.986 CPU sec, 3.681 real sec
sorting block 59, #reads = 1075793 mem = 536881817 peak = 1050640384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277969, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 378, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (395.64, 171.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670664 sequences (100000281 bp) in 2.435 CPU sec, 2.603 real sec...
[M::process] read 670618 sequences (100000056 bp) in 1.560 CPU sec, 1.565 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 276882, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 387, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (401.26, 170.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671484 reads in 173.278 CPU sec, 5.518 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 276950, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 388, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (402.37, 170.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670558 sequences (100000186 bp) in 2.343 CPU sec, 2.351 real sec...
[M::mem_process_seqs] Processed 670664 reads in 92.721 CPU sec, 2.924 real sec
sorting block 60, #reads = 1076308 mem = 536880422 peak = 1050640384
[M::mem_process_seqs] Processed 670666 reads in 228.840 CPU sec, 7.258 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 276924, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 388, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (402.34, 170.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 61, #reads = 1075770 mem = 536882120 peak = 1050640384
[M::process] read 670790 sequences (100000215 bp) in 2.317 CPU sec, 2.442 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277181, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 386, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (400.79, 169.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670838 sequences (100000282 bp) in 1.830 CPU sec, 1.836 real sec...
[M::mem_process_seqs] Processed 670618 reads in 186.013 CPU sec, 5.888 real sec
[M::mem_process_seqs] Processed 670558 reads in 105.812 CPU sec, 3.342 real sec
sorting block 62, #reads = 1074651 mem = 536880571 peak = 1050640384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277193, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 383, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (397.55, 167.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670752 sequences (100000231 bp) in 2.432 CPU sec, 2.594 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 276745, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 381, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (396.91, 169.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670676 sequences (100000227 bp) in 1.999 CPU sec, 2.005 real sec...
[M::mem_process_seqs] Processed 670790 reads in 171.452 CPU sec, 5.437 real sec
[M::mem_process_seqs] Processed 670838 reads in 108.983 CPU sec, 3.449 real sec
sorting block 63, #reads = 1075803 mem = 536879934 peak = 1050640384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277108, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 383, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (397.41, 168.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670774 sequences (100000221 bp) in 2.472 CPU sec, 2.627 real sec...
[M::process] read 670722 sequences (100000300 bp) in 1.830 CPU sec, 1.838 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277352, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 382, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (397.48, 168.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670752 reads in 173.611 CPU sec, 5.509 real sec
[M::mem_process_seqs] Processed 670676 reads in 107.243 CPU sec, 3.391 real sec
sorting block 64, #reads = 1074546 mem = 536882355 peak = 1050640384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 276610, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 381, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (396.05, 168.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670802 sequences (100000065 bp) in 2.737 CPU sec, 2.859 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 276542, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 381, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (396.30, 167.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670648 sequences (100000199 bp) in 1.890 CPU sec, 1.898 real sec...
[M::mem_process_seqs] Processed 670774 reads in 171.791 CPU sec, 5.446 real sec
sorting block 65, #reads = 1076127 mem = 536878945 peak = 1050640384
[M::mem_process_seqs] Processed 670722 reads in 107.624 CPU sec, 3.398 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 276739, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 380, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (395.22, 166.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 66, #reads = 1074586 mem = 536877331 peak = 1050640384
[M::process] read 670542 sequences (100000035 bp) in 2.526 CPU sec, 2.661 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277016, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 381, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (395.88, 166.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670462 sequences (100000240 bp) in 2.065 CPU sec, 2.066 real sec...
[M::mem_process_seqs] Processed 670802 reads in 175.149 CPU sec, 5.558 real sec
[M::mem_process_seqs] Processed 670648 reads in 107.854 CPU sec, 3.397 real sec
sorting block 67, #reads = 1073759 mem = 536879547 peak = 1050640384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 277339, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 382, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (397.18, 167.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670684 sequences (100000236 bp) in 2.979 CPU sec, 3.128 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277437, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 379, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (395.31, 167.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670406 sequences (100000290 bp) in 1.859 CPU sec, 1.854 real sec...
[M::mem_process_seqs] Processed 670542 reads in 170.559 CPU sec, 5.401 real sec
[M::mem_process_seqs] Processed 670462 reads in 105.403 CPU sec, 3.324 real sec
sorting block 68, #reads = 1074584 mem = 536877781 peak = 1050640384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277300, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 378, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (393.84, 165.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670140 sequences (100000225 bp) in 2.616 CPU sec, 2.742 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277344, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 382, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (397.78, 167.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670684 reads in 161.957 CPU sec, 5.135 real sec
[M::process] read 669984 sequences (100000222 bp) in 1.850 CPU sec, 1.848 real sec...
[M::mem_process_seqs] Processed 670406 reads in 99.824 CPU sec, 3.143 real sec
sorting block 69, #reads = 1075663 mem = 536878308 peak = 1050640384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277859, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 385, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (401.16, 168.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670108 sequences (100000136 bp) in 2.810 CPU sec, 2.942 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277311, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 391, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (406.01, 171.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670140 reads in 157.649 CPU sec, 4.996 real sec
[M::mem_process_seqs] Processed 669984 reads in 99.060 CPU sec, 3.115 real sec
[M::process] read 674052 sequences (100000213 bp) in 2.199 CPU sec, 2.215 real sec...
sorting block 70, #reads = 1075741 mem = 536886084 peak = 1050640384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 277761, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (275, 395, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (410.56, 175.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 71, #reads = 1075167 mem = 536880272 peak = 1050640384
[M::process] read 673272 sequences (100000218 bp) in 2.546 CPU sec, 2.671 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278665, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 352, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (376.01, 171.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670108 reads in 161.389 CPU sec, 5.131 real sec
[M::mem_process_seqs] Processed 674052 reads in 104.747 CPU sec, 3.293 real sec
[M::process] read 672738 sequences (100000199 bp) in 2.000 CPU sec, 2.028 real sec...
sorting block 72, #reads = 1072862 mem = 536879988 peak = 1050640384
[M::process] read 672432 sequences (100000012 bp) in 2.661 CPU sec, 2.793 real sec...
[M::process] read 672188 sequences (100000142 bp) in 1.520 CPU sec, 1.517 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278776, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 369, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (387.54, 172.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672738 reads in 91.810 CPU sec, 2.900 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 278859, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 364, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (383.86, 173.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 279200, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 370, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (387.54, 170.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671900 sequences (100000030 bp) in 1.756 CPU sec, 1.760 real sec...
[M::mem_process_seqs] Processed 673272 reads in 249.556 CPU sec, 7.920 real sec
sorting block 73, #reads = 1074404 mem = 536883669 peak = 1050640384
[M::mem_process_seqs] Processed 672432 reads in 113.998 CPU sec, 3.630 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278482, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 374, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (391.29, 171.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 74, #reads = 1074160 mem = 536880807 peak = 1078153216
[M::process] read 671700 sequences (100000235 bp) in 2.322 CPU sec, 2.479 real sec...
[M::process] read 671220 sequences (100000217 bp) in 1.580 CPU sec, 1.579 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278397, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 379, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (394.90, 172.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672188 reads in 166.713 CPU sec, 5.309 real sec
[M::mem_process_seqs] Processed 671900 reads in 106.642 CPU sec, 3.383 real sec
sorting block 75, #reads = 1073723 mem = 536880796 peak = 1078153216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278040, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 384, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040)
[M::mem_pestat] mean and std.dev: (398.43, 174.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1300)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671266 sequences (100000264 bp) in 2.983 CPU sec, 3.158 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277704, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 387, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (401.79, 173.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1300)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671700 reads in 160.587 CPU sec, 5.075 real sec
[M::process] read 671278 sequences (100000196 bp) in 2.065 CPU sec, 2.086 real sec...
[M::mem_process_seqs] Processed 671220 reads in 99.563 CPU sec, 3.124 real sec
sorting block 76, #reads = 1072435 mem = 536886451 peak = 1078153216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 277614, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 388, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (401.49, 173.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1300)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671090 sequences (100000021 bp) in 2.142 CPU sec, 2.258 real sec...
[M::process] read 670846 sequences (100000127 bp) in 1.510 CPU sec, 1.509 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278730, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 388, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037)
[M::mem_pestat] mean and std.dev: (401.74, 172.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671266 reads in 160.275 CPU sec, 5.102 real sec
sorting block 77, #reads = 1073578 mem = 536879329 peak = 1078153216
[M::mem_process_seqs] Processed 671278 reads in 99.697 CPU sec, 3.154 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 277839, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 390, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046)
[M::mem_pestat] mean and std.dev: (403.37, 173.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1306)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670938 sequences (100000093 bp) in 2.881 CPU sec, 3.005 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277943, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 391, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044)
[M::mem_pestat] mean and std.dev: (404.52, 173.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1303)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670902 sequences (100000100 bp) in 1.970 CPU sec, 1.968 real sec...
[M::mem_process_seqs] Processed 671090 reads in 163.488 CPU sec, 5.163 real sec
sorting block 78, #reads = 1072872 mem = 536879925 peak = 1078153216
[M::mem_process_seqs] Processed 670846 reads in 102.313 CPU sec, 3.224 real sec
sorting block 79, #reads = 1071934 mem = 536879402 peak = 1078153216
[M::process] read 670906 sequences (100000112 bp) in 2.563 CPU sec, 2.686 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277663, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 670980 sequences (100000180 bp) in 1.580 CPU sec, 1.578 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (267, 387, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (401.17, 171.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670902 reads in 93.997 CPU sec, 2.967 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278599, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 389, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (403.28, 172.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277626, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 387, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (401.39, 171.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670980 sequences (100000060 bp) in 1.665 CPU sec, 1.672 real sec...
[M::mem_process_seqs] Processed 670938 reads in 254.367 CPU sec, 8.082 real sec
sorting block 80, #reads = 1072075 mem = 536878397 peak = 1078153216
[M::mem_process_seqs] Processed 670906 reads in 117.022 CPU sec, 3.730 real sec
sorting block 81, #reads = 1072670 mem = 536876549 peak = 1078153216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277567, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 386, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (400.93, 171.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671054 sequences (100000191 bp) in 2.733 CPU sec, 2.895 real sec...
[M::process] read 671036 sequences (100000051 bp) in 1.500 CPU sec, 1.508 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 277570, 6, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (5514, 5552, 5992)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (4558, 6948)
[M::mem_pestat] mean and std.dev: (5682.75, 269.16)
[M::mem_pestat] low and high boundaries for proper pairs: (4080, 7426)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 385, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (400.00, 170.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 670980 reads in 168.888 CPU sec, 5.362 real sec
[M::mem_process_seqs] Processed 670980 reads in 106.052 CPU sec, 3.359 real sec
sorting block 82, #reads = 1072202 mem = 536879741 peak = 1078153216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 278093, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 383, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (397.70, 169.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670800 sequences (100000242 bp) in 2.492 CPU sec, 2.648 real sec...
[M::process] read 670818 sequences (100000107 bp) in 1.590 CPU sec, 1.592 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277626, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 384, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (398.32, 170.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1280)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671054 reads in 169.371 CPU sec, 5.374 real sec
[M::mem_process_seqs] Processed 671036 reads in 104.970 CPU sec, 3.333 real sec
sorting block 83, #reads = 1072085 mem = 536884286 peak = 1078153216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 278479, 2, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (1282, 5738, 5738)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14650)
[M::mem_pestat] mean and std.dev: (3787.80, 2507.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 19106)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 386, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (399.21, 168.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 671052 sequences (100000123 bp) in 2.443 CPU sec, 2.563 real sec...
[M::process] read 670868 sequences (100000258 bp) in 1.520 CPU sec, 1.520 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277601, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 380, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (395.28, 167.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670800 reads in 165.690 CPU sec, 5.265 real sec
sorting block 84, #reads = 1070937 mem = 536878553 peak = 1078153216
[M::mem_process_seqs] Processed 670818 reads in 104.133 CPU sec, 3.303 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278212, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 377, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (392.95, 166.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670850 sequences (100000049 bp) in 2.464 CPU sec, 2.597 real sec...
[M::process] read 670764 sequences (100000274 bp) in 1.670 CPU sec, 1.668 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 277642, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 377, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (392.89, 166.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671052 reads in 160.688 CPU sec, 5.098 real sec
sorting block 85, #reads = 1072416 mem = 536884002 peak = 1078153216
[M::mem_process_seqs] Processed 670868 reads in 99.634 CPU sec, 3.150 real sec
sorting block 86, #reads = 1071841 mem = 536877653 peak = 1092194304
[M::process] read 670854 sequences (100000128 bp) in 2.233 CPU sec, 2.354 real sec...
[M::process] read 672134 sequences (100000255 bp) in 1.520 CPU sec, 1.520 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278656, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 382, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (398.47, 170.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670764 reads in 84.133 CPU sec, 2.656 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 278436, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 381, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (396.18, 168.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278714, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 385, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (402.48, 173.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674604 sequences (100000084 bp) in 1.641 CPU sec, 1.651 real sec...
[M::mem_process_seqs] Processed 670850 reads in 241.236 CPU sec, 7.657 real sec
sorting block 87, #reads = 1072103 mem = 536877856 peak = 1092194304
[M::mem_process_seqs] Processed 670854 reads in 109.304 CPU sec, 3.486 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278900, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 373, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (393.02, 175.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 88, #reads = 1071999 mem = 536880385 peak = 1092194304
[M::process] read 673712 sequences (100000202 bp) in 2.773 CPU sec, 2.935 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280907, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 343, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (365.68, 165.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672134 reads in 160.309 CPU sec, 5.083 real sec
[M::process] read 673166 sequences (100000084 bp) in 1.890 CPU sec, 1.912 real sec...
[M::mem_process_seqs] Processed 674604 reads in 100.274 CPU sec, 3.152 real sec
sorting block 89, #reads = 1070532 mem = 536882664 peak = 1092194304
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279765, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 353, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (372.93, 165.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672976 sequences (100000041 bp) in 2.618 CPU sec, 2.782 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279174, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 359, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (376.82, 165.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673060 sequences (100000115 bp) in 1.490 CPU sec, 1.487 real sec...
[M::mem_process_seqs] Processed 673712 reads in 155.924 CPU sec, 4.947 real sec
[M::mem_process_seqs] Processed 673166 reads in 97.276 CPU sec, 3.066 real sec
sorting block 90, #reads = 1071251 mem = 536882479 peak = 1092194304
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280030, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 363, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (380.19, 166.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673050 sequences (100000138 bp) in 2.716 CPU sec, 2.870 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280062, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 361, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (376.88, 164.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672574 sequences (100000239 bp) in 1.690 CPU sec, 1.687 real sec...
[M::mem_process_seqs] Processed 672976 reads in 156.675 CPU sec, 4.961 real sec
sorting block 91, #reads = 1072085 mem = 536878369 peak = 1092194304
[M::process] read 672088 sequences (100000047 bp) in 1.886 CPU sec, 1.891 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279566, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 364, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (380.69, 167.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673050 reads in 87.941 CPU sec, 2.782 real sec
[M::mem_process_seqs] Processed 673060 reads in 209.969 CPU sec, 6.650 real sec
sorting block 92, #reads = 1069272 mem = 536877930 peak = 1092194304
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279483, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 370, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (386.89, 169.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671878 sequences (100000255 bp) in 2.403 CPU sec, 2.533 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279119, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 373, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (388.55, 168.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671992 sequences (100000127 bp) in 1.460 CPU sec, 1.463 real sec...
[M::mem_process_seqs] Processed 672574 reads in 169.391 CPU sec, 5.377 real sec
[M::mem_process_seqs] Processed 672088 reads in 98.256 CPU sec, 3.106 real sec
sorting block 93, #reads = 1070927 mem = 536877378 peak = 1092194304
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 279023, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 376, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (390.21, 168.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671540 sequences (100000286 bp) in 2.554 CPU sec, 2.695 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279871, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 379, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (393.60, 170.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671878 reads in 154.467 CPU sec, 4.891 real sec
[M::process] read 671412 sequences (100000158 bp) in 1.825 CPU sec, 1.826 real sec...
[M::mem_process_seqs] Processed 671992 reads in 96.045 CPU sec, 3.019 real sec
sorting block 94, #reads = 1071351 mem = 536879870 peak = 1092194304
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279276, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 383, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (398.72, 173.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671132 sequences (100000064 bp) in 2.483 CPU sec, 2.624 real sec...
[M::process] read 671162 sequences (100000146 bp) in 1.530 CPU sec, 1.525 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278365, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 387, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (400.19, 172.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671540 reads in 156.995 CPU sec, 4.988 real sec
sorting block 95, #reads = 1072061 mem = 536879176 peak = 1092194304
[M::mem_process_seqs] Processed 671412 reads in 99.195 CPU sec, 3.134 real sec
sorting block 96, #reads = 1070935 mem = 536879023 peak = 1138470912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279319, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 388, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040)
[M::mem_pestat] mean and std.dev: (401.96, 172.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1298)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671090 sequences (100000086 bp) in 2.616 CPU sec, 2.728 real sec...
[M::process] read 670912 sequences (100000282 bp) in 1.550 CPU sec, 1.545 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278787, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 386, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (401.23, 172.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1297)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671132 reads in 154.839 CPU sec, 4.906 real sec
[M::mem_process_seqs] Processed 671162 reads in 96.153 CPU sec, 3.036 real sec
sorting block 97, #reads = 1071112 mem = 536877666 peak = 1138470912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278608, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 386, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (400.93, 173.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1303)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671078 sequences (100000020 bp) in 2.873 CPU sec, 2.997 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278561, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 388, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (402.98, 174.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1302)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671090 reads in 156.315 CPU sec, 4.951 real sec
[M::mem_process_seqs] Processed 670912 reads in 97.829 CPU sec, 3.074 real sec
[M::process] read 670804 sequences (100000094 bp) in 2.060 CPU sec, 2.086 real sec...
sorting block 98, #reads = 1071469 mem = 536880311 peak = 1138470912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278279, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 386, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (401.84, 173.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1297)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670924 sequences (100000056 bp) in 2.286 CPU sec, 2.411 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278863, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 670860 sequences (100000274 bp) in 1.470 CPU sec, 1.472 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (267, 387, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (402.77, 173.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671078 reads in 152.547 CPU sec, 4.854 real sec
sorting block 99, #reads = 1071012 mem = 536878017 peak = 1138470912
[M::mem_process_seqs] Processed 670804 reads in 93.787 CPU sec, 2.967 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278034, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 391, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056)
[M::mem_pestat] mean and std.dev: (406.54, 176.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1319)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 100, #reads = 1072559 mem = 536879608 peak = 1138470912
[M::process] read 670678 sequences (100000050 bp) in 2.918 CPU sec, 3.033 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278332, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 391, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054)
[M::mem_pestat] mean and std.dev: (407.26, 177.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1316)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670924 reads in 152.779 CPU sec, 4.842 real sec
[M::mem_process_seqs] Processed 670860 reads in 96.851 CPU sec, 3.045 real sec
[M::process] read 670426 sequences (100000262 bp) in 2.253 CPU sec, 2.312 real sec...
sorting block 101, #reads = 1071966 mem = 536879896 peak = 1138470912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277920, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 394, 534)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1064)
[M::mem_pestat] mean and std.dev: (410.60, 180.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1329)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670592 sequences (100000026 bp) in 2.039 CPU sec, 2.147 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278584, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 397, 539)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075)
[M::mem_pestat] mean and std.dev: (414.11, 181.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1343)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670468 sequences (100000032 bp) in 1.500 CPU sec, 1.500 real sec...
[M::mem_process_seqs] Processed 670678 reads in 151.969 CPU sec, 4.833 real sec
[M::mem_process_seqs] Processed 670426 reads in 94.658 CPU sec, 2.990 real sec
sorting block 102, #reads = 1072605 mem = 536880625 peak = 1138470912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278674, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 400, 545)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093)
[M::mem_pestat] mean and std.dev: (417.75, 185.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1367)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672562 sequences (100000132 bp) in 3.037 CPU sec, 3.168 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 278693, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 400, 547)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1099)
[M::mem_pestat] mean and std.dev: (418.95, 187.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1375)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670592 reads in 154.721 CPU sec, 4.894 real sec
[M::mem_process_seqs] Processed 670468 reads in 99.643 CPU sec, 3.129 real sec
[M::process] read 673302 sequences (100000077 bp) in 2.029 CPU sec, 2.080 real sec...
sorting block 103, #reads = 1072282 mem = 536877723 peak = 1138470912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279011, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 375, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048)
[M::mem_pestat] mean and std.dev: (395.29, 179.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1313)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672778 sequences (100000150 bp) in 2.683 CPU sec, 2.799 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 279160, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 358, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (377.92, 168.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672406 sequences (100000248 bp) in 1.910 CPU sec, 1.912 real sec...
[M::mem_process_seqs] Processed 672562 reads in 169.663 CPU sec, 5.376 real sec
sorting block 104, #reads = 1069135 mem = 536881821 peak = 1138470912
[M::mem_process_seqs] Processed 673302 reads in 101.316 CPU sec, 3.190 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278449, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 362, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (379.87, 166.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 105, #reads = 1069666 mem = 536879113 peak = 1138470912
[M::process] read 672354 sequences (100000156 bp) in 2.974 CPU sec, 3.121 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278641, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 365, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (382.25, 166.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672778 reads in 155.503 CPU sec, 4.920 real sec
[M::mem_process_seqs] Processed 672406 reads in 96.552 CPU sec, 3.033 real sec
[M::process] read 672278 sequences (100000199 bp) in 2.132 CPU sec, 2.170 real sec...
sorting block 106, #reads = 1074457 mem = 536877431 peak = 1138470912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278463, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 366, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (383.16, 166.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672086 sequences (100000068 bp) in 2.695 CPU sec, 2.820 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278433, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 369, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (383.92, 165.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672354 reads in 156.998 CPU sec, 4.970 real sec
[M::process] read 671760 sequences (100000208 bp) in 2.081 CPU sec, 2.101 real sec...
[M::mem_process_seqs] Processed 672278 reads in 99.761 CPU sec, 3.128 real sec
sorting block 107, #reads = 1073544 mem = 536884823 peak = 1138470912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 278583, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 373, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (388.90, 168.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671694 sequences (100000245 bp) in 2.896 CPU sec, 3.039 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278100, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 377, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (392.24, 169.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672086 reads in 160.621 CPU sec, 5.103 real sec
[M::mem_process_seqs] Processed 671760 reads in 100.089 CPU sec, 3.145 real sec
[M::process] read 671746 sequences (100000034 bp) in 2.152 CPU sec, 2.194 real sec...
sorting block 108, #reads = 1072847 mem = 536883552 peak = 1138470912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277933, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 381, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (394.90, 170.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1280)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671850 sequences (100000027 bp) in 2.654 CPU sec, 2.754 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278248, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 379, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (393.66, 169.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671442 sequences (100000066 bp) in 1.930 CPU sec, 1.931 real sec...
[M::mem_process_seqs] Processed 671694 reads in 173.810 CPU sec, 5.509 real sec
sorting block 109, #reads = 1073854 mem = 536877930 peak = 1138470912
[M::mem_process_seqs] Processed 671746 reads in 107.244 CPU sec, 3.375 real sec
sorting block 110, #reads = 1071652 mem = 536881245 peak = 1138470912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278647, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 379, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (392.18, 168.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671422 sequences (100000273 bp) in 2.781 CPU sec, 2.904 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277815, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 383, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (395.75, 169.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671258 sequences (100000043 bp) in 1.930 CPU sec, 1.929 real sec...
[M::mem_process_seqs] Processed 671850 reads in 169.183 CPU sec, 5.357 real sec
[M::mem_process_seqs] Processed 671442 reads in 106.663 CPU sec, 3.359 real sec
sorting block 111, #reads = 1071701 mem = 536875992 peak = 1138470912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 277690, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 385, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (398.21, 170.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670982 sequences (100000010 bp) in 2.843 CPU sec, 2.943 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 277913, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 385, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (397.46, 168.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670792 sequences (100000165 bp) in 1.970 CPU sec, 1.972 real sec...
[M::mem_process_seqs] Processed 671422 reads in 169.724 CPU sec, 5.367 real sec
[M::mem_process_seqs] Processed 671258 reads in 103.285 CPU sec, 3.249 real sec
sorting block 112, #reads = 1071609 mem = 536878188 peak = 1138470912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277746, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 388, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (400.16, 169.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670892 sequences (100000225 bp) in 2.890 CPU sec, 3.006 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277890, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 387, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (400.50, 169.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670816 sequences (100000181 bp) in 1.960 CPU sec, 1.957 real sec...
[M::mem_process_seqs] Processed 670982 reads in 174.175 CPU sec, 5.514 real sec
[M::mem_process_seqs] Processed 670792 reads in 108.217 CPU sec, 3.410 real sec
sorting block 113, #reads = 1072324 mem = 536878884 peak = 1138470912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278124, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 385, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (399.57, 170.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670948 sequences (100000001 bp) in 2.680 CPU sec, 2.786 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278034, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 385, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (400.26, 171.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670916 sequences (100000138 bp) in 2.000 CPU sec, 2.000 real sec...
[M::mem_process_seqs] Processed 670892 reads in 170.626 CPU sec, 5.401 real sec
sorting block 114, #reads = 1071333 mem = 536879360 peak = 1138470912
[M::mem_process_seqs] Processed 670816 reads in 106.018 CPU sec, 3.332 real sec
sorting block 115, #reads = 1071836 mem = 536880262 peak = 1138470912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277706, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 384, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (398.83, 170.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670958 sequences (100000153 bp) in 2.751 CPU sec, 2.854 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278146, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 386, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (400.71, 172.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1296)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670966 sequences (100000212 bp) in 1.990 CPU sec, 1.993 real sec...
[M::mem_process_seqs] Processed 670948 reads in 172.055 CPU sec, 5.442 real sec
[M::mem_process_seqs] Processed 670916 reads in 107.528 CPU sec, 3.393 real sec
sorting block 116, #reads = 1071169 mem = 536876694 peak = 1138470912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278293, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 383, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (398.89, 171.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670946 sequences (100000095 bp) in 2.644 CPU sec, 2.736 real sec...
[M::process] read 670970 sequences (100000197 bp) in 1.970 CPU sec, 1.971 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277758, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 385, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (399.95, 171.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670958 reads in 174.990 CPU sec, 5.541 real sec
[M::mem_process_seqs] Processed 670966 reads in 108.193 CPU sec, 3.422 real sec
sorting block 117, #reads = 1068146 mem = 536880316 peak = 1138470912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278558, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 384, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (399.57, 171.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670962 sequences (100000271 bp) in 2.602 CPU sec, 2.705 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278487, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 386, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042)
[M::mem_pestat] mean and std.dev: (402.01, 173.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1301)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670908 sequences (100000123 bp) in 1.960 CPU sec, 1.962 real sec...
[M::mem_process_seqs] Processed 670946 reads in 166.161 CPU sec, 5.258 real sec
[M::mem_process_seqs] Processed 670970 reads in 102.860 CPU sec, 3.241 real sec
sorting block 118, #reads = 1070258 mem = 536878435 peak = 1138470912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278790, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 388, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046)
[M::mem_pestat] mean and std.dev: (403.70, 174.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1306)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673426 sequences (100000084 bp) in 2.891 CPU sec, 2.989 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278395, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 388, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060)
[M::mem_pestat] mean and std.dev: (406.26, 178.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1325)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674916 sequences (100000046 bp) in 1.990 CPU sec, 1.988 real sec...
[M::mem_process_seqs] Processed 670962 reads in 172.264 CPU sec, 5.450 real sec
sorting block 119, #reads = 1070416 mem = 536880061 peak = 1138470912
[M::mem_process_seqs] Processed 670908 reads in 110.413 CPU sec, 3.481 real sec
sorting block 120, #reads = 1068016 mem = 536883539 peak = 1138470912
[M::process] read 674230 sequences (100000220 bp) in 2.891 CPU sec, 2.973 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280593, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 673500 sequences (100000017 bp) in 1.920 CPU sec, 1.927 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (233, 339, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (359.45, 160.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674916 reads in 90.342 CPU sec, 2.843 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279900, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 359, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (380.37, 171.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280361, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 348, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (367.15, 161.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673426 reads in 263.824 CPU sec, 8.330 real sec
[M::process] read 673640 sequences (100000267 bp) in 2.355 CPU sec, 2.357 real sec...
sorting block 121, #reads = 1066779 mem = 536879266 peak = 1138470912
[M::mem_process_seqs] Processed 674230 reads in 119.839 CPU sec, 3.797 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279590, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 354, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (371.55, 161.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 122, #reads = 1068692 mem = 536879459 peak = 1138470912
[M::process] read 674370 sequences (100000280 bp) in 3.177 CPU sec, 3.324 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279827, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 352, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (370.20, 162.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673500 reads in 163.311 CPU sec, 5.165 real sec
[M::process] read 673320 sequences (100000187 bp) in 1.953 CPU sec, 1.978 real sec...
[M::mem_process_seqs] Processed 673640 reads in 103.063 CPU sec, 3.236 real sec
sorting block 123, #reads = 1070146 mem = 536877600 peak = 1138470912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280132, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 346, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (363.97, 159.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673420 sequences (100000036 bp) in 2.078 CPU sec, 2.179 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279941, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 672506 sequences (100000141 bp) in 1.970 CPU sec, 1.964 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (245, 358, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (374.97, 164.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674370 reads in 167.972 CPU sec, 5.333 real sec
[M::mem_process_seqs] Processed 673320 reads in 103.004 CPU sec, 3.253 real sec
sorting block 124, #reads = 1068057 mem = 536879723 peak = 1138470912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278909, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 354, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (373.98, 165.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672464 sequences (100000099 bp) in 2.542 CPU sec, 2.656 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278454, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 371, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (386.47, 167.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671964 sequences (100000288 bp) in 1.920 CPU sec, 1.926 real sec...
[M::mem_process_seqs] Processed 673420 reads in 162.474 CPU sec, 5.144 real sec
[M::mem_process_seqs] Processed 672506 reads in 101.733 CPU sec, 3.208 real sec
sorting block 125, #reads = 1069699 mem = 536877071 peak = 1138470912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278871, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 370, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (385.08, 166.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671566 sequences (100000200 bp) in 2.497 CPU sec, 2.620 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 278720, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 379, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (391.98, 168.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671476 sequences (100000168 bp) in 1.980 CPU sec, 1.981 real sec...
[M::mem_process_seqs] Processed 672464 reads in 164.963 CPU sec, 5.221 real sec
sorting block 126, #reads = 1069349 mem = 536880076 peak = 1138470912
[M::mem_process_seqs] Processed 671964 reads in 103.233 CPU sec, 3.252 real sec
sorting block 127, #reads = 1068585 mem = 536877146 peak = 1138470912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 278579, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 381, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (394.87, 169.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671170 sequences (100000208 bp) in 2.880 CPU sec, 2.982 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278430, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 381, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (393.45, 167.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671566 reads in 164.692 CPU sec, 5.207 real sec
[M::process] read 671414 sequences (100000045 bp) in 1.970 CPU sec, 1.967 real sec...
[M::mem_process_seqs] Processed 671476 reads in 104.503 CPU sec, 3.282 real sec
sorting block 128, #reads = 1068966 mem = 536879213 peak = 1138470912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278289, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 383, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (395.05, 166.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671046 sequences (100000267 bp) in 2.225 CPU sec, 2.339 real sec...
[M::process] read 671054 sequences (100000127 bp) in 1.530 CPU sec, 1.530 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278040, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 382, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (395.67, 169.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671170 reads in 164.824 CPU sec, 5.240 real sec
[M::mem_process_seqs] Processed 671414 reads in 102.210 CPU sec, 3.245 real sec
sorting block 129, #reads = 1067102 mem = 536880968 peak = 1138470912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278264, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 385, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040)
[M::mem_pestat] mean and std.dev: (399.86, 172.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671000 sequences (100000176 bp) in 2.444 CPU sec, 2.577 real sec...
[M::process] read 670724 sequences (100000122 bp) in 1.510 CPU sec, 1.507 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278086, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 384, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (400.21, 172.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1303)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671046 reads in 160.682 CPU sec, 5.103 real sec
[M::mem_process_seqs] Processed 671054 reads in 99.528 CPU sec, 3.159 real sec
sorting block 130, #reads = 1070385 mem = 536879477 peak = 1138470912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277384, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 384, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (398.18, 169.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670732 sequences (100000194 bp) in 2.359 CPU sec, 2.471 real sec...
[M::process] read 670612 sequences (100000192 bp) in 1.530 CPU sec, 1.529 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277582, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 385, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (398.99, 168.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671000 reads in 162.719 CPU sec, 5.172 real sec
sorting block 131, #reads = 1071645 mem = 536879576 peak = 1138470912
[M::mem_process_seqs] Processed 670724 reads in 101.680 CPU sec, 3.220 real sec
sorting block 132, #reads = 1070840 mem = 536879402 peak = 1138470912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277881, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 386, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (400.45, 170.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670520 sequences (100000084 bp) in 2.464 CPU sec, 2.562 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277754, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 386, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (401.15, 170.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670542 sequences (100000298 bp) in 1.950 CPU sec, 1.955 real sec...
[M::mem_process_seqs] Processed 670732 reads in 160.143 CPU sec, 5.068 real sec
[M::mem_process_seqs] Processed 670612 reads in 98.650 CPU sec, 3.111 real sec
sorting block 133, #reads = 1072711 mem = 536882316 peak = 1138470912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277814, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 389, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (403.83, 171.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1296)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670432 sequences (100000199 bp) in 2.593 CPU sec, 2.699 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277998, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 393, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050)
[M::mem_pestat] mean and std.dev: (407.87, 174.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1310)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 431982 sequences (64368963 bp) in 1.460 CPU sec, 1.461 real sec...
[M::mem_process_seqs] Processed 670520 reads in 159.350 CPU sec, 5.059 real sec
[M::mem_process_seqs] Processed 670542 reads in 98.188 CPU sec, 3.100 real sec
sorting block 134, #reads = 1072300 mem = 536880925 peak = 1138470912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278387, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 395, 534)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062)
[M::mem_pestat] mean and std.dev: (410.31, 177.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1326)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 179606, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 384, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (401.14, 173.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670432 reads in 130.323 CPU sec, 4.217 real sec
[M::mem_process_seqs] Processed 431982 reads in 70.144 CPU sec, 2.265 real sec
sorting block 135, #reads = 1071748 mem = 536878001 peak = 1138470912
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 146088854
overall: 69777555456 mem 20588.218 user 439.154 sys 693.546 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_Kh-T1-TDNA-1_A23MHWWLT4_1.L002\tPU:L002\tSM:659_Kh-T1-TDNA-1_A23MHWWLT4_1\tLB:659_Kh-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_Kh-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_Kh-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 693.583 sec; CPU: 21027.375 sec
sorting block 136, #reads = 793351 mem = 397885795 peak = 1138470912
uniq header count 1
merging 137 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 8206044263
output reads: 146801634
bam_mem_sort: 137 calls 74.813 user 1.524 sys 76.970 real
bam_write: 137 calls 33.277 user 1.881 sys 41.011 real
execute: 1 calls 42.102 user 1.171 sys 745.476 real
merge_files: 1 calls 42.073 user 1.150 sys 51.377 real
parse_chunk: 2188 calls 93.866 user 23.305 sys 121.475 real
read_chunk: 5893 calls 42.126 user 42.309 sys 631.632 real
sort_block: 1 calls 31.260 user 41.640 sys 612.527 real
write_chunk: 3703 calls 41.682 user 0.939 sys 50.540 real
overall: 3258638336 mem 2020.464 user 82.886 sys 745.497 real
ls: cannot access '*.cram': No such file or directory