This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_3U6-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1842.972 CPU sec, 550.231 real sec
[M::process] read 687060 sequences (100000198 bp) in 0.990 CPU sec, 0.999 real sec...
[M::process] read 686120 sequences (100000162 bp) in 1.230 CPU sec, 1.224 real sec...
[M::process] read 684726 sequences (100000269 bp) in 1.182 CPU sec, 1.187 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 284315, 8, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 276, 570)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1394)
[M::mem_pestat] mean and std.dev: (276.90, 161.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1806)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 300, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (320.79, 160.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 306, 893)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2147)
[M::mem_pestat] mean and std.dev: (366.62, 230.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2774)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683674 sequences (100000137 bp) in 1.134 CPU sec, 1.153 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283929, 2, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (125, 196, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (279.53, 184.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 315, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (332.12, 163.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 262, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598)
[M::mem_pestat] mean and std.dev: (264.79, 85.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 284075, 9, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (217, 283, 681)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1609)
[M::mem_pestat] mean and std.dev: (339.31, 205.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2073)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 305, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (324.58, 161.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 314, 1848)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5040)
[M::mem_pestat] mean and std.dev: (1004.70, 1101.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6636)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687060 reads in 224.405 CPU sec, 7.190 real sec
[M::mem_process_seqs] Processed 684726 reads in 149.635 CPU sec, 4.847 real sec
[M::mem_process_seqs] Processed 686120 reads in 161.802 CPU sec, 5.257 real sec
sorting block 0, #reads = 1082481 mem = 536878757 peak = 594153472
[M::process] read 683484 sequences (100000172 bp) in 1.792 CPU sec, 1.972 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283506, 9, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (234, 415, 2056)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5700)
[M::mem_pestat] mean and std.dev: (1061.54, 1228.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7522)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 323, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (339.85, 167.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 383, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (367.00, 132.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683248 sequences (100000200 bp) in 1.226 CPU sec, 1.244 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 283142, 4, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (236, 293, 629)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1415)
[M::mem_pestat] mean and std.dev: (397.14, 269.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1808)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 324, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (340.71, 167.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (278, 752, 1138)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2858)
[M::mem_pestat] mean and std.dev: (694.71, 422.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3718)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683156 sequences (100000125 bp) in 1.121 CPU sec, 1.128 real sec...
[M::mem_process_seqs] Processed 683674 reads in 138.890 CPU sec, 4.418 real sec
sorting block 1, #reads = 1080712 mem = 536883012 peak = 802353152
[M::mem_process_seqs] Processed 683484 reads in 86.937 CPU sec, 2.754 real sec
sorting block 2, #reads = 1081257 mem = 536878942 peak = 980054016
[M::process] read 683130 sequences (100000116 bp) in 1.611 CPU sec, 1.714 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282524, 7, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (227, 352, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (335.18, 110.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 322, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (339.35, 165.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 288, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (278.20, 113.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682456 sequences (100000004 bp) in 1.220 CPU sec, 1.229 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282222, 10, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 259, 906)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2422)
[M::mem_pestat] mean and std.dev: (374.92, 424.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3180)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 320, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (337.48, 164.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (42, 51, 104)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 228)
[M::mem_pestat] mean and std.dev: (64.80, 40.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 290)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 338, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (398.22, 221.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 282064, 3, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 299, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (291.67, 150.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 321, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (339.11, 166.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 290, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (300.21, 141.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683248 reads in 201.131 CPU sec, 6.453 real sec
[M::mem_process_seqs] Processed 683156 reads in 148.239 CPU sec, 4.774 real sec
[M::mem_process_seqs] Processed 683130 reads in 160.425 CPU sec, 5.189 real sec
sorting block 3, #reads = 1079699 mem = 536881347 peak = 980054016
[M::process] read 681916 sequences (100000278 bp) in 1.969 CPU sec, 2.104 real sec...
sorting block 4, #reads = 1080466 mem = 536880522 peak = 980054016
[M::process] read 681380 sequences (100000171 bp) in 1.505 CPU sec, 1.584 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282187, 9, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 209, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1163)
[M::mem_pestat] mean and std.dev: (324.20, 219.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1488)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 328, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (343.57, 165.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 378, 700)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1620)
[M::mem_pestat] mean and std.dev: (406.83, 277.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2080)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681916 reads in 77.633 CPU sec, 2.452 real sec
[M::process] read 683682 sequences (100000197 bp) in 1.521 CPU sec, 1.575 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282191, 7, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 255, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (262.82, 105.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 324, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (341.11, 166.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 291, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (301.14, 100.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 282053, 5, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 291, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (270.73, 99.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 335, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (349.87, 168.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 686470 sequences (100000151 bp) in 1.228 CPU sec, 1.235 real sec...
[M::mem_process_seqs] Processed 682456 reads in 217.594 CPU sec, 6.944 real sec
sorting block 5, #reads = 1078627 mem = 536879120 peak = 980054016
[M::mem_process_seqs] Processed 681380 reads in 100.286 CPU sec, 3.196 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282640, 5, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 226, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605)
[M::mem_pestat] mean and std.dev: (228.29, 82.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 748)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 322, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (338.29, 166.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 360, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (325.54, 147.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685672 sequences (100000094 bp) in 1.760 CPU sec, 1.906 real sec...
[M::process] read 684286 sequences (100000175 bp) in 1.240 CPU sec, 1.244 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283713, 5, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 264, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (352.26, 252.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1364)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 307, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (326.58, 163.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 349, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (311.45, 164.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1291)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683682 reads in 140.351 CPU sec, 4.487 real sec
sorting block 6, #reads = 1078738 mem = 536880955 peak = 980054016
[M::mem_process_seqs] Processed 686470 reads in 86.404 CPU sec, 2.759 real sec
sorting block 7, #reads = 1081610 mem = 536882341 peak = 980054016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283034, 2, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (217, 285, 775)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1891)
[M::mem_pestat] mean and std.dev: (357.13, 229.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2449)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 312, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (330.09, 164.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (327, 356, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (247, 447)
[M::mem_pestat] mean and std.dev: (343.59, 16.34)
[M::mem_pestat] low and high boundaries for proper pairs: (207, 487)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683582 sequences (100000211 bp) in 1.963 CPU sec, 2.097 real sec...
[M::process] read 683588 sequences (100000300 bp) in 1.280 CPU sec, 1.274 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 283142, 2, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (250, 357, 623)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1369)
[M::mem_pestat] mean and std.dev: (420.11, 234.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1742)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 320, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (336.65, 165.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 282, 765)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1893)
[M::mem_pestat] mean and std.dev: (383.36, 344.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2457)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685672 reads in 140.209 CPU sec, 4.477 real sec
[M::mem_process_seqs] Processed 684286 reads in 86.941 CPU sec, 2.771 real sec
sorting block 8, #reads = 1082740 mem = 536877902 peak = 980054016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282890, 19, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (270, 270, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (329.29, 162.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 327, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (342.95, 168.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (581, 989, 1931)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4631)
[M::mem_pestat] mean and std.dev: (917.88, 593.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5981)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 465, 545)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1213)
[M::mem_pestat] mean and std.dev: (375.82, 159.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1547)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 683570 sequences (100000180 bp) in 1.768 CPU sec, 1.883 real sec...
[M::process] read 683742 sequences (100000108 bp) in 1.300 CPU sec, 1.306 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282218, 5, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 308, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (329.35, 223.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 326, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (342.86, 169.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 322, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (300.55, 106.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683582 reads in 142.261 CPU sec, 4.557 real sec
[M::mem_process_seqs] Processed 683588 reads in 88.968 CPU sec, 2.838 real sec
sorting block 9, #reads = 1081105 mem = 536878969 peak = 980054016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283385, 8, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 190, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (339.71, 282.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1469)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 322, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (340.02, 167.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 346, 676)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1576)
[M::mem_pestat] mean and std.dev: (394.68, 195.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2026)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683132 sequences (100000098 bp) in 2.091 CPU sec, 2.235 real sec...
[M::process] read 682386 sequences (100000003 bp) in 1.330 CPU sec, 1.327 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 282917, 3, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 255, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (293.45, 150.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 316, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (334.35, 163.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 252, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (242.00, 121.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683570 reads in 142.772 CPU sec, 4.560 real sec
sorting block 10, #reads = 1079363 mem = 536877433 peak = 980054016
[M::mem_process_seqs] Processed 683742 reads in 88.513 CPU sec, 2.823 real sec
sorting block 11, #reads = 1079583 mem = 536876443 peak = 980054016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 282475, 3, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 217, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (285.11, 153.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1298)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 320, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (336.51, 163.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 250, 709)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1819)
[M::mem_pestat] mean and std.dev: (403.20, 394.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2374)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682252 sequences (100000230 bp) in 1.857 CPU sec, 1.983 real sec...
[M::process] read 681702 sequences (100000289 bp) in 1.360 CPU sec, 1.368 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282986, 4, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (260, 370, 947)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2321)
[M::mem_pestat] mean and std.dev: (536.07, 466.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3008)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 324, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (340.84, 165.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 388, 540)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1092)
[M::mem_pestat] mean and std.dev: (377.92, 181.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1368)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683132 reads in 143.985 CPU sec, 4.616 real sec
[M::process] read 687552 sequences (100000105 bp) in 1.528 CPU sec, 1.533 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282649, 1, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 280, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (280.61, 129.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 328, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (344.68, 168.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 266, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (258.47, 84.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 749)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682252 reads in 81.084 CPU sec, 2.756 real sec
sorting block 12, #reads = 1079944 mem = 536883806 peak = 980054016
[M::mem_process_seqs] Processed 682386 reads in 193.123 CPU sec, 6.346 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 282746, 8, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (197, 289, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1196)
[M::mem_pestat] mean and std.dev: (278.20, 133.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1529)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 333, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (350.09, 171.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 152, 213)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 353)
[M::mem_pestat] mean and std.dev: (177.44, 61.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 424)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687030 sequences (100000198 bp) in 1.893 CPU sec, 2.014 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 284121, 5, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (280, 484, 883)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2089)
[M::mem_pestat] mean and std.dev: (452.38, 293.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2692)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 303, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (322.46, 161.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 685634 sequences (100000097 bp) in 1.350 CPU sec, 1.352 real sec...
[M::mem_process_seqs] Processed 681702 reads in 154.772 CPU sec, 4.940 real sec
sorting block 13, #reads = 1079407 mem = 536879848 peak = 980054016
[M::mem_process_seqs] Processed 687552 reads in 90.807 CPU sec, 2.886 real sec
sorting block 14, #reads = 1080311 mem = 536877774 peak = 1004126208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 284248, 3, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (211, 228, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (276.40, 139.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 303, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (320.90, 158.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 332, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (230.00, 88.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 752)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684670 sequences (100000291 bp) in 1.960 CPU sec, 2.102 real sec...
[M::process] read 683812 sequences (100000250 bp) in 1.340 CPU sec, 1.337 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 283437, 7, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 203, 254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456)
[M::mem_pestat] mean and std.dev: (191.08, 54.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 557)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 311, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (326.91, 159.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 311, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (297.96, 156.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687030 reads in 147.272 CPU sec, 4.716 real sec
[M::mem_process_seqs] Processed 685634 reads in 90.660 CPU sec, 2.890 real sec
sorting block 15, #reads = 1081906 mem = 536879851 peak = 1004126208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283266, 6, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (241, 299, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (340.04, 150.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 317, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (332.11, 161.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 242, 536)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1278)
[M::mem_pestat] mean and std.dev: (340.00, 233.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1649)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683420 sequences (100000076 bp) in 1.928 CPU sec, 2.080 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 281917, 4, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (216, 301, 871)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2181)
[M::mem_pestat] mean and std.dev: (601.06, 543.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2836)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 321, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (335.62, 162.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 356, 1325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3451)
[M::mem_pestat] mean and std.dev: (773.50, 757.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4514)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683572 sequences (100000222 bp) in 1.360 CPU sec, 1.353 real sec...
[M::mem_process_seqs] Processed 684670 reads in 142.001 CPU sec, 4.539 real sec
[M::mem_process_seqs] Processed 683812 reads in 88.248 CPU sec, 2.802 real sec
sorting block 16, #reads = 1081374 mem = 536878977 peak = 1004126208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282444, 5, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (201, 307, 549)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1245)
[M::mem_pestat] mean and std.dev: (387.80, 268.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1593)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 322, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (337.42, 163.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 294, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (325.71, 139.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682980 sequences (100000296 bp) in 2.193 CPU sec, 2.329 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282536, 3, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 238, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1217)
[M::mem_pestat] mean and std.dev: (297.87, 173.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1559)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 682966 sequences (100000168 bp) in 1.330 CPU sec, 1.338 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (201, 319, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (335.43, 163.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 304, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593)
[M::mem_pestat] mean and std.dev: (252.75, 105.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 728)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683420 reads in 138.813 CPU sec, 4.413 real sec
sorting block 17, #reads = 1080033 mem = 536876744 peak = 1004126208
[M::mem_process_seqs] Processed 683572 reads in 87.193 CPU sec, 2.764 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 282462, 6, 19)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 321, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (337.06, 162.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (130, 145, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (203.18, 127.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] skip orientation RR
sorting block 18, #reads = 1080014 mem = 536878056 peak = 1004126208
[M::process] read 682622 sequences (100000055 bp) in 2.003 CPU sec, 2.147 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 282291, 8, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 227, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (310.09, 201.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 321, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (336.85, 162.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 337, 660)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1576)
[M::mem_pestat] mean and std.dev: (461.41, 347.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2034)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682280 sequences (100000107 bp) in 1.360 CPU sec, 1.361 real sec...
[M::mem_process_seqs] Processed 682980 reads in 141.410 CPU sec, 4.504 real sec
[M::mem_process_seqs] Processed 682966 reads in 89.356 CPU sec, 2.837 real sec
sorting block 19, #reads = 1079037 mem = 536880392 peak = 1004126208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282563, 11, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (318, 377, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (354.30, 119.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1098)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 325, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (341.05, 165.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (350, 1164, 1164)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2792)
[M::mem_pestat] mean and std.dev: (832.55, 473.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3606)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 301, 715)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1835)
[M::mem_pestat] mean and std.dev: (326.47, 226.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2395)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 685746 sequences (100000064 bp) in 2.168 CPU sec, 2.285 real sec...
[M::process] read 686704 sequences (100000025 bp) in 1.330 CPU sec, 1.330 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282766, 1, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (239, 378, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (343.00, 142.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 963)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 327, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (344.25, 168.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 336, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066)
[M::mem_pestat] mean and std.dev: (372.10, 229.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1351)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682622 reads in 138.054 CPU sec, 4.395 real sec
[M::mem_process_seqs] Processed 682280 reads in 86.572 CPU sec, 2.756 real sec
sorting block 20, #reads = 1078861 mem = 536879587 peak = 1004126208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 283565, 3, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (249, 382, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (327.36, 119.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 310, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (329.01, 163.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 220, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (249.93, 121.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685658 sequences (100000205 bp) in 2.021 CPU sec, 2.164 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283837, 2, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (216, 474, 1807)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4989)
[M::mem_pestat] mean and std.dev: (847.18, 781.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6580)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 306, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (324.13, 160.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 290, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (266.33, 110.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684838 sequences (100000046 bp) in 1.330 CPU sec, 1.328 real sec...
[M::mem_process_seqs] Processed 685746 reads in 137.933 CPU sec, 4.391 real sec
[M::mem_process_seqs] Processed 686704 reads in 86.355 CPU sec, 2.737 real sec
sorting block 21, #reads = 1077651 mem = 536881290 peak = 1004126208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283069, 12, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 288, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862)
[M::mem_pestat] mean and std.dev: (261.35, 100.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1085)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 310, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (326.70, 160.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (832, 1135, 1953)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4195)
[M::mem_pestat] mean and std.dev: (1272.75, 839.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5316)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 220, 816)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2152)
[M::mem_pestat] mean and std.dev: (311.00, 240.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2820)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 684126 sequences (100000181 bp) in 2.140 CPU sec, 2.284 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 282694, 5, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 206, 237)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (18, 383)
[M::mem_pestat] mean and std.dev: (195.92, 43.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 456)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 317, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (333.29, 162.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 275, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (321.47, 173.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683834 sequences (100000000 bp) in 1.330 CPU sec, 1.333 real sec...
[M::mem_process_seqs] Processed 685658 reads in 140.135 CPU sec, 4.462 real sec
sorting block 22, #reads = 1083061 mem = 536880031 peak = 1012576256
[M::mem_process_seqs] Processed 684838 reads in 88.341 CPU sec, 2.802 real sec
sorting block 23, #reads = 1081446 mem = 536881158 peak = 1046917120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 282391, 6, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (251, 290, 1057)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2669)
[M::mem_pestat] mean and std.dev: (499.27, 376.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3475)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 320, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (334.72, 162.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 409, 798)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1954)
[M::mem_pestat] mean and std.dev: (357.00, 199.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2532)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685110 sequences (100000013 bp) in 2.059 CPU sec, 2.198 real sec...
[M::process] read 684102 sequences (100000022 bp) in 1.360 CPU sec, 1.362 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282307, 2, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 281, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1061)
[M::mem_pestat] mean and std.dev: (349.42, 208.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1355)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 320, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (335.95, 163.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 406, 1865)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5113)
[M::mem_pestat] mean and std.dev: (803.49, 756.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6737)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684126 reads in 142.886 CPU sec, 4.551 real sec
[M::mem_process_seqs] Processed 683834 reads in 89.779 CPU sec, 2.846 real sec
sorting block 24, #reads = 1080311 mem = 536878869 peak = 1046917120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 283345, 0, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 183, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (236.00, 121.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 310, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (328.73, 161.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 377, 3181)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9095)
[M::mem_pestat] mean and std.dev: (1372.95, 1655.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12052)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683670 sequences (100000016 bp) in 2.282 CPU sec, 2.402 real sec...
[M::process] read 683460 sequences (100000052 bp) in 1.330 CPU sec, 1.334 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 282709, 4, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 313, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (319.45, 205.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 312, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (328.81, 159.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 274, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873)
[M::mem_pestat] mean and std.dev: (263.09, 124.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1107)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685110 reads in 141.104 CPU sec, 4.484 real sec
[M::mem_process_seqs] Processed 684102 reads in 88.490 CPU sec, 2.806 real sec
sorting block 25, #reads = 1080121 mem = 536879264 peak = 1046917120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 282540, 5, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 317, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (286.00, 139.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 314, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (329.66, 158.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 594, 1105)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3021)
[M::mem_pestat] mean and std.dev: (734.64, 628.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3979)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682896 sequences (100000014 bp) in 1.967 CPU sec, 2.072 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282577, 1, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 293, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (300.60, 114.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 316, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (331.07, 158.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 358, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (346.19, 155.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686146 sequences (100000153 bp) in 1.370 CPU sec, 1.363 real sec...
[M::mem_process_seqs] Processed 683670 reads in 140.643 CPU sec, 4.483 real sec
[M::mem_process_seqs] Processed 683460 reads in 88.090 CPU sec, 2.792 real sec
sorting block 26, #reads = 1079346 mem = 536880117 peak = 1046917120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282775, 8, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (242, 306, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (329.44, 129.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 322, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (338.34, 164.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 408, 1219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3179)
[M::mem_pestat] mean and std.dev: (708.33, 671.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4159)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 27, #reads = 1077906 mem = 536877951 peak = 1080385536
[M::process] read 689892 sequences (100000178 bp) in 2.132 CPU sec, 2.250 real sec...
[M::process] read 686882 sequences (100000219 bp) in 1.380 CPU sec, 1.376 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 283697, 9, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 205, 670)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1756)
[M::mem_pestat] mean and std.dev: (475.50, 482.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2407)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 305, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (327.52, 165.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 241, 699)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1743)
[M::mem_pestat] mean and std.dev: (384.64, 318.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2265)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682896 reads in 139.803 CPU sec, 4.444 real sec
[M::mem_process_seqs] Processed 686146 reads in 88.646 CPU sec, 2.813 real sec
sorting block 28, #reads = 1079017 mem = 536879502 peak = 1080385536
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 285397, 0, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 211, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (306.53, 200.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 285, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (312.56, 161.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 263, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527)
[M::mem_pestat] mean and std.dev: (265.79, 121.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685656 sequences (100000153 bp) in 1.972 CPU sec, 2.071 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 284111, 6, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 269, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (250.75, 99.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 856)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 303, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (326.46, 166.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 685012 sequences (100000065 bp) in 1.360 CPU sec, 1.356 real sec...
[M::mem_process_seqs] Processed 689892 reads in 140.967 CPU sec, 4.493 real sec
[M::mem_process_seqs] Processed 686882 reads in 87.590 CPU sec, 2.775 real sec
sorting block 29, #reads = 1080983 mem = 536878830 peak = 1080385536
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283621, 5, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 219, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 423)
[M::mem_pestat] mean and std.dev: (203.33, 59.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 506)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 311, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (331.78, 166.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 272, 849)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2009)
[M::mem_pestat] mean and std.dev: (470.79, 302.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2589)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684212 sequences (100000149 bp) in 2.290 CPU sec, 2.393 real sec...
[M::process] read 684010 sequences (100000086 bp) in 1.360 CPU sec, 1.359 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 283704, 5, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 162, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1087)
[M::mem_pestat] mean and std.dev: (292.08, 274.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1401)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 316, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (334.84, 166.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 412, 757)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1895)
[M::mem_pestat] mean and std.dev: (459.62, 300.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2464)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685656 reads in 141.287 CPU sec, 4.490 real sec
[M::mem_process_seqs] Processed 685012 reads in 89.760 CPU sec, 2.850 real sec
sorting block 30, #reads = 1080773 mem = 536879545 peak = 1089126400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283360, 3, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 426, 542)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1252)
[M::mem_pestat] mean and std.dev: (427.95, 283.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1607)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 319, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (336.45, 165.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 292, 670)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1596)
[M::mem_pestat] mean and std.dev: (408.10, 336.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2059)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683624 sequences (100000074 bp) in 2.049 CPU sec, 2.161 real sec...
[M::process] read 683622 sequences (100000228 bp) in 1.360 CPU sec, 1.360 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 283344, 5, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (235, 314, 603)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1339)
[M::mem_pestat] mean and std.dev: (428.58, 291.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1707)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 319, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (336.47, 165.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 310, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (247.00, 90.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 951)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684212 reads in 145.198 CPU sec, 4.627 real sec
sorting block 31, #reads = 1079013 mem = 536881455 peak = 1089126400
[M::mem_process_seqs] Processed 684010 reads in 91.478 CPU sec, 2.901 real sec
sorting block 32, #reads = 1077678 mem = 536878084 peak = 1121415168
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283166, 4, 30)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 318, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (335.77, 164.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 329, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055)
[M::mem_pestat] mean and std.dev: (371.86, 165.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1329)
[M::mem_pestat] skip orientation RR
[M::process] read 683230 sequences (100000205 bp) in 2.084 CPU sec, 2.197 real sec...
[M::process] read 682900 sequences (100000163 bp) in 1.370 CPU sec, 1.366 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282977, 4, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (263, 350, 567)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1175)
[M::mem_pestat] mean and std.dev: (375.58, 212.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1479)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 319, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (336.28, 164.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 513, 886)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2126)
[M::mem_pestat] mean and std.dev: (733.21, 603.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3146)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683624 reads in 145.828 CPU sec, 4.642 real sec
[M::mem_process_seqs] Processed 683622 reads in 92.041 CPU sec, 2.924 real sec
sorting block 33, #reads = 1076467 mem = 536878705 peak = 1121415168
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 282858, 9, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 286, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (307.26, 158.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 320, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (336.96, 164.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 802, 885)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2183)
[M::mem_pestat] mean and std.dev: (642.50, 417.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2832)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 689962 sequences (100000198 bp) in 2.132 CPU sec, 2.265 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 283571, 6, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 194, 587)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1429)
[M::mem_pestat] mean and std.dev: (349.71, 331.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1850)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 325, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (342.16, 167.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 420, 843)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2127)
[M::mem_pestat] mean and std.dev: (561.14, 480.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2769)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 692604 sequences (100000071 bp) in 1.420 CPU sec, 1.421 real sec...
[M::mem_process_seqs] Processed 683230 reads in 143.984 CPU sec, 4.581 real sec
[M::mem_process_seqs] Processed 682900 reads in 90.579 CPU sec, 2.866 real sec
sorting block 34, #reads = 1075142 mem = 536887816 peak = 1121415168
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 285311, 3, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (270, 330, 878)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2094)
[M::mem_pestat] mean and std.dev: (434.07, 321.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2702)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 282, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (309.79, 159.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 248, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718)
[M::mem_pestat] mean and std.dev: (286.54, 130.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 689690 sequences (100000107 bp) in 2.317 CPU sec, 2.407 real sec...
[M::process] read 687756 sequences (100000023 bp) in 1.390 CPU sec, 1.389 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 286864, 5, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 235, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491)
[M::mem_pestat] mean and std.dev: (205.75, 81.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 612)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 268, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (295.83, 150.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1073)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 371, 676)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1610)
[M::mem_pestat] mean and std.dev: (368.18, 225.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2077)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 689962 reads in 150.104 CPU sec, 4.777 real sec
[M::mem_process_seqs] Processed 692604 reads in 94.618 CPU sec, 3.008 real sec
sorting block 35, #reads = 1076704 mem = 536879987 peak = 1121415168
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 286142, 7, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (613, 664, 961)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1657)
[M::mem_pestat] mean and std.dev: (566.38, 242.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2005)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 286, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (309.21, 154.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 321, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (13, 628)
[M::mem_pestat] mean and std.dev: (295.93, 80.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 751)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687180 sequences (100000258 bp) in 2.056 CPU sec, 2.163 real sec...
[M::process] read 685630 sequences (100000270 bp) in 1.410 CPU sec, 1.412 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285614, 0, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 153, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537)
[M::mem_pestat] mean and std.dev: (200.82, 95.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 665)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 296, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (317.27, 158.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 259, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724)
[M::mem_pestat] mean and std.dev: (271.85, 85.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 689690 reads in 146.790 CPU sec, 4.675 real sec
sorting block 36, #reads = 1079639 mem = 536880135 peak = 1121415168
[M::mem_process_seqs] Processed 687756 reads in 91.784 CPU sec, 2.910 real sec
sorting block 37, #reads = 1078395 mem = 536878550 peak = 1152475136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 285411, 9, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (213, 617, 1183)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3123)
[M::mem_pestat] mean and std.dev: (655.47, 570.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4093)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 302, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (321.24, 159.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (304, 502, 648)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1336)
[M::mem_pestat] mean and std.dev: (440.17, 229.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1680)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684708 sequences (100000293 bp) in 2.314 CPU sec, 2.403 real sec...
[M::process] read 683912 sequences (100000165 bp) in 1.380 CPU sec, 1.379 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 284278, 3, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 180, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1294)
[M::mem_pestat] mean and std.dev: (217.36, 137.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1681)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 310, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (328.99, 162.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 279, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (241.09, 85.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687180 reads in 148.292 CPU sec, 4.715 real sec
[M::mem_process_seqs] Processed 685630 reads in 93.544 CPU sec, 2.966 real sec
sorting block 38, #reads = 1077009 mem = 536875349 peak = 1152475136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 283714, 3, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 298, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (331.95, 189.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 315, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (332.75, 163.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 299, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (324.94, 174.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683416 sequences (100000009 bp) in 2.057 CPU sec, 2.193 real sec...
[M::process] read 682616 sequences (100000256 bp) in 1.370 CPU sec, 1.366 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 283174, 4, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 254, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1105)
[M::mem_pestat] mean and std.dev: (275.50, 124.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1409)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 317, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (336.25, 165.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 276, 714)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1752)
[M::mem_pestat] mean and std.dev: (415.07, 262.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2271)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684708 reads in 147.892 CPU sec, 4.711 real sec
[M::mem_process_seqs] Processed 683912 reads in 92.699 CPU sec, 2.942 real sec
sorting block 39, #reads = 1076096 mem = 536879076 peak = 1152475136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 283270, 2, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 369, 542)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1242)
[M::mem_pestat] mean and std.dev: (410.76, 297.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1601)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 320, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (337.83, 165.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 377, 684)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1574)
[M::mem_pestat] mean and std.dev: (456.84, 358.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2019)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 688498 sequences (100000013 bp) in 2.373 CPU sec, 2.498 real sec...
[M::process] read 690250 sequences (100000147 bp) in 1.360 CPU sec, 1.354 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282610, 3, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 263, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1186)
[M::mem_pestat] mean and std.dev: (287.93, 240.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1521)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 326, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (343.22, 168.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 314, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (313.17, 101.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683416 reads in 144.003 CPU sec, 4.574 real sec
sorting block 40, #reads = 1076314 mem = 536877082 peak = 1152475136
[M::mem_process_seqs] Processed 682616 reads in 89.268 CPU sec, 2.829 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 284760, 4, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 280, 546)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1324)
[M::mem_pestat] mean and std.dev: (282.64, 190.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1713)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 291, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (316.49, 161.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 312, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1100)
[M::mem_pestat] mean and std.dev: (273.35, 134.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1412)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 41, #reads = 1077255 mem = 536876114 peak = 1152475136
[M::process] read 687702 sequences (100000293 bp) in 2.083 CPU sec, 2.202 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284892, 2, 17)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 283, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881)
[M::mem_pestat] mean and std.dev: (307.59, 155.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 316, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1065)
[M::mem_pestat] mean and std.dev: (308.12, 168.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1361)
[M::mem_pestat] skip orientation RR
[M::process] read 686468 sequences (100000166 bp) in 1.450 CPU sec, 1.453 real sec...
[M::mem_process_seqs] Processed 688498 reads in 146.899 CPU sec, 4.680 real sec
[M::mem_process_seqs] Processed 690250 reads in 89.966 CPU sec, 2.852 real sec
sorting block 42, #reads = 1079167 mem = 536877984 peak = 1152475136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284358, 2, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (229, 273, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (85, 445)
[M::mem_pestat] mean and std.dev: (247.82, 57.93)
[M::mem_pestat] low and high boundaries for proper pairs: (13, 517)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 296, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (316.03, 156.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 238, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (286.24, 177.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1098)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686384 sequences (100000185 bp) in 2.310 CPU sec, 2.399 real sec...
[M::process] read 685888 sequences (100000247 bp) in 1.380 CPU sec, 1.376 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283854, 4, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 275, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (278.38, 148.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 304, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (321.71, 157.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 263, 1498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4080)
[M::mem_pestat] mean and std.dev: (643.06, 633.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5371)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687702 reads in 141.006 CPU sec, 4.481 real sec
[M::mem_process_seqs] Processed 686468 reads in 89.082 CPU sec, 2.827 real sec
sorting block 43, #reads = 1083219 mem = 536882136 peak = 1152475136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284162, 3, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 305, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (323.12, 158.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 458, 557)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1139)
[M::mem_pestat] mean and std.dev: (456.21, 198.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1430)
[M::mem_pestat] skip orientation RR
[M::process] read 685666 sequences (100000269 bp) in 2.052 CPU sec, 2.133 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 283947, 8, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 289, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (252.39, 100.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 684714 sequences (100000159 bp) in 1.420 CPU sec, 1.423 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (193, 308, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (324.05, 157.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 277, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (258.35, 108.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686384 reads in 141.044 CPU sec, 4.490 real sec
sorting block 44, #reads = 1080065 mem = 536877628 peak = 1152475136
[M::process] read 684368 sequences (100000290 bp) in 1.652 CPU sec, 1.660 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 284144, 3, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (253, 434, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868)
[M::mem_pestat] mean and std.dev: (401.67, 195.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 309, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (326.48, 159.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 358, 538)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1100)
[M::mem_pestat] mean and std.dev: (387.00, 155.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1381)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685666 reads in 86.177 CPU sec, 2.996 real sec
[M::mem_process_seqs] Processed 685888 reads in 199.673 CPU sec, 6.610 real sec
sorting block 45, #reads = 1079025 mem = 536877105 peak = 1152475136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282848, 3, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 303, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (250.23, 99.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 919)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 315, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (331.53, 161.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (303, 370, 582)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1140)
[M::mem_pestat] mean and std.dev: (369.54, 143.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1419)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684040 sequences (100000021 bp) in 1.996 CPU sec, 2.096 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 283326, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (214, 350, 1016)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2620)
[M::mem_pestat] mean and std.dev: (596.03, 628.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3422)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 312, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (329.53, 160.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 683630 sequences (100000298 bp) in 1.380 CPU sec, 1.377 real sec...
[M::mem_process_seqs] Processed 684714 reads in 161.974 CPU sec, 5.145 real sec
[M::mem_process_seqs] Processed 684368 reads in 95.134 CPU sec, 3.016 real sec
sorting block 46, #reads = 1078567 mem = 536880381 peak = 1152475136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 283052, 10, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 301, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (285.89, 116.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 317, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (334.68, 163.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (78, 474, 1182)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3390)
[M::mem_pestat] mean and std.dev: (617.20, 575.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4494)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 348, 587)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1329)
[M::mem_pestat] mean and std.dev: (380.64, 216.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1700)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695158 sequences (100000213 bp) in 2.474 CPU sec, 2.637 real sec...
[M::process] read 691868 sequences (100000174 bp) in 1.410 CPU sec, 1.416 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283091, 7, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 300, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (298.10, 207.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1326)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 318, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (336.66, 165.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 355, 600)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1312)
[M::mem_pestat] mean and std.dev: (401.18, 261.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1668)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684040 reads in 152.904 CPU sec, 4.856 real sec
sorting block 47, #reads = 1075134 mem = 536879100 peak = 1152475136
[M::mem_process_seqs] Processed 683630 reads in 99.841 CPU sec, 3.172 real sec
sorting block 48, #reads = 1072722 mem = 536879431 peak = 1174069248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 287655, 2, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 434, 560)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1318)
[M::mem_pestat] mean and std.dev: (406.09, 263.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1697)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 257, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (286.78, 147.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 289, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (319.13, 162.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1083)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 689348 sequences (100000148 bp) in 2.307 CPU sec, 2.417 real sec...
[M::process] read 688188 sequences (100000073 bp) in 1.390 CPU sec, 1.385 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286675, 9, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 275, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (298.17, 148.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1075)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 252, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (295.77, 193.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1096)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695158 reads in 152.137 CPU sec, 4.835 real sec
[M::mem_process_seqs] Processed 691868 reads in 95.192 CPU sec, 3.029 real sec
sorting block 49, #reads = 1076857 mem = 536877419 peak = 1174069248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 285592, 1, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (278, 370, 962)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2330)
[M::mem_pestat] mean and std.dev: (350.00, 183.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3014)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 288, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881)
[M::mem_pestat] mean and std.dev: (308.96, 152.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 460, 972)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2592)
[M::mem_pestat] mean and std.dev: (524.69, 417.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3402)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687966 sequences (100000056 bp) in 2.285 CPU sec, 2.390 real sec...
[M::process] read 685634 sequences (100000238 bp) in 1.400 CPU sec, 1.399 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285522, 1, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (264, 355, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (362.10, 167.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 297, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (316.24, 155.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 194, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608)
[M::mem_pestat] mean and std.dev: (228.00, 97.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 759)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 689348 reads in 150.378 CPU sec, 4.782 real sec
[M::mem_process_seqs] Processed 688188 reads in 94.649 CPU sec, 3.006 real sec
sorting block 50, #reads = 1076876 mem = 536877350 peak = 1174069248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 284834, 4, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (218, 359, 598)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1358)
[M::mem_pestat] mean and std.dev: (364.84, 161.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1738)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 296, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (316.62, 157.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 336, 930)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2422)
[M::mem_pestat] mean and std.dev: (558.45, 630.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3168)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684450 sequences (100000030 bp) in 2.249 CPU sec, 2.354 real sec...
[M::process] read 684144 sequences (100000273 bp) in 1.350 CPU sec, 1.343 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283895, 5, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 244, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (251.46, 96.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 872)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 306, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (322.67, 156.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 264, 556)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1252)
[M::mem_pestat] mean and std.dev: (280.00, 174.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1600)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687966 reads in 150.499 CPU sec, 4.788 real sec
[M::mem_process_seqs] Processed 685634 reads in 94.984 CPU sec, 3.017 real sec
sorting block 51, #reads = 1076335 mem = 536876252 peak = 1174069248
[M::process] read 683060 sequences (100000090 bp) in 2.039 CPU sec, 2.137 real sec...
[M::process] read 682600 sequences (100000081 bp) in 1.350 CPU sec, 1.345 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282812, 6, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (197, 320, 564)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1298)
[M::mem_pestat] mean and std.dev: (393.14, 293.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1665)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 309, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (325.59, 156.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 332, 539)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1163)
[M::mem_pestat] mean and std.dev: (390.48, 227.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1475)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684144 reads in 83.986 CPU sec, 2.653 real sec
sorting block 52, #reads = 1075532 mem = 536878789 peak = 1193549824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283425, 7, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 217, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860)
[M::mem_pestat] mean and std.dev: (246.33, 151.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1091)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 310, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (326.33, 157.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 281, 596)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1492)
[M::mem_pestat] mean and std.dev: (314.31, 213.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1940)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 1762 sequences (255874 bp) in 0.072 CPU sec, 0.081 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282844, 0, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (212, 221, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596)
[M::mem_pestat] mean and std.dev: (249.11, 107.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 724)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 318, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (335.64, 162.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 1414, 1915)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5431)
[M::mem_pestat] mean and std.dev: (1007.57, 867.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7189)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684450 reads in 224.206 CPU sec, 7.183 real sec
[M::mem_process_seqs] Processed 683060 reads in 102.825 CPU sec, 3.330 real sec
sorting block 53, #reads = 1075514 mem = 536881514 peak = 1193549824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 738, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 318, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (331.21, 161.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1762 reads in 0.505 CPU sec, 0.017 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282640, 1, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (199, 321, 638)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1516)
[M::mem_pestat] mean and std.dev: (385.96, 221.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1955)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 324, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (342.07, 168.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 323, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (297.70, 130.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682600 reads in 78.616 CPU sec, 2.597 real sec
sorting block 54, #reads = 1077370 mem = 536883444 peak = 1196761088
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 59605542
overall: 69876953088 mem 8266.452 user 401.509 sys 777.510 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_3U6-T1-TDNA-1_A23MHWWLT4_1.L004\tPU:L004\tSM:1136_3U6-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_3U6-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0004.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0004.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 777.547 sec; CPU: 8667.964 sec
sorting block 55, #reads = 579926 mem = 289441730 peak = 1196761088
uniq header count 1
merging 56 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3579728217
output reads: 59923904
bam_mem_sort: 56 calls 19.717 user 0.317 sys 20.204 real
bam_write: 56 calls 43.872 user 0.953 sys 45.067 real
execute: 1 calls 12.055 user 0.999 sys 798.184 real
merge_files: 1 calls 12.037 user 0.971 sys 19.842 real
parse_chunk: 889 calls 27.125 user 7.930 sys 35.855 real
read_chunk: 2400 calls 22.594 user 21.531 sys 227.457 real
sort_block: 1 calls 17.531 user 20.934 sys 219.896 real
write_chunk: 1509 calls 11.663 user 0.781 sys 19.054 real
overall: 2702639104 mem 734.765 user 39.428 sys 798.206 real
ls: cannot access '*.cram': No such file or directory