This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_bR4-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2341.070 CPU sec, 113.729 real sec
[M::process] read 678164 sequences (100000227 bp) in 1.725 CPU sec, 1.727 real sec...
[M::process] read 677644 sequences (100000264 bp) in 1.919 CPU sec, 2.082 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282330, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 327, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (342.94, 153.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677358 sequences (100000078 bp) in 1.906 CPU sec, 2.303 real sec...
[M::process] read 677146 sequences (100000179 bp) in 1.914 CPU sec, 2.298 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281568, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 330, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (346.98, 156.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678164 reads in 241.426 CPU sec, 8.902 real sec
[M::mem_process_seqs] Processed 677644 reads in 155.559 CPU sec, 5.974 real sec
sorting block 0, #reads = 1080685 mem = 536878613 peak = 581402624
[M::process] read 676880 sequences (100000266 bp) in 2.431 CPU sec, 2.887 real sec...
[M::process] read 676560 sequences (100000021 bp) in 2.105 CPU sec, 2.215 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281000, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 334, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (348.94, 155.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677146 reads in 132.750 CPU sec, 4.650 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281324, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 333, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (348.38, 155.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281472, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 336, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (350.87, 155.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677358 reads in 368.337 CPU sec, 12.688 real sec
[M::process] read 676300 sequences (100000213 bp) in 2.047 CPU sec, 2.111 real sec...
sorting block 1, #reads = 1080285 mem = 536878456 peak = 719814656
[M::mem_process_seqs] Processed 676880 reads in 179.177 CPU sec, 6.376 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281737, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 339, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (353.93, 156.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676296 sequences (100000208 bp) in 2.529 CPU sec, 3.101 real sec...
sorting block 2, #reads = 1080671 mem = 536885208 peak = 938033152
[M::process] read 675922 sequences (100000011 bp) in 2.192 CPU sec, 2.342 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281116, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 341, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (355.15, 156.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676560 reads in 240.167 CPU sec, 8.214 real sec
[M::mem_process_seqs] Processed 676300 reads in 155.823 CPU sec, 5.324 real sec
sorting block 3, #reads = 1080294 mem = 536878998 peak = 938033152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281282, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 340, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (354.34, 156.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675662 sequences (100000068 bp) in 2.580 CPU sec, 3.122 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281323, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 342, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (355.92, 156.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675470 sequences (100000019 bp) in 2.148 CPU sec, 2.259 real sec...
[M::mem_process_seqs] Processed 676296 reads in 240.217 CPU sec, 8.130 real sec
[M::mem_process_seqs] Processed 675922 reads in 156.679 CPU sec, 5.415 real sec
sorting block 4, #reads = 1080379 mem = 536878053 peak = 938033152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281291, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 343, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (357.62, 156.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675368 sequences (100000191 bp) in 2.836 CPU sec, 3.445 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281724, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 348, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (361.70, 158.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675662 reads in 241.513 CPU sec, 8.099 real sec
[M::process] read 674780 sequences (100000252 bp) in 2.216 CPU sec, 2.220 real sec...
[M::mem_process_seqs] Processed 675470 reads in 157.412 CPU sec, 5.254 real sec
sorting block 5, #reads = 1079267 mem = 536879118 peak = 938033152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281005, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 348, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (361.72, 158.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675086 sequences (100000046 bp) in 3.023 CPU sec, 3.443 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280814, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 351, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (365.07, 158.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675368 reads in 246.845 CPU sec, 8.325 real sec
[M::process] read 675030 sequences (100000050 bp) in 2.259 CPU sec, 2.406 real sec...
sorting block 6, #reads = 1079386 mem = 536881938 peak = 938033152
[M::mem_process_seqs] Processed 674780 reads in 157.227 CPU sec, 5.330 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281182, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 348, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (361.34, 157.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674880 sequences (100000233 bp) in 3.085 CPU sec, 3.550 real sec...
sorting block 7, #reads = 1079972 mem = 536885698 peak = 961601536
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280816, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 349, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (362.46, 157.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674986 sequences (100000008 bp) in 2.327 CPU sec, 2.485 real sec...
[M::mem_process_seqs] Processed 675086 reads in 252.387 CPU sec, 9.262 real sec
[M::mem_process_seqs] Processed 675030 reads in 164.226 CPU sec, 6.109 real sec
sorting block 8, #reads = 1079747 mem = 536879873 peak = 961601536
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281089, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 349, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (362.27, 156.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675078 sequences (100000029 bp) in 3.377 CPU sec, 3.727 real sec...
[M::process] read 675114 sequences (100000133 bp) in 2.411 CPU sec, 2.426 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 281073, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 348, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (361.93, 157.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674880 reads in 262.013 CPU sec, 9.351 real sec
[M::mem_process_seqs] Processed 674986 reads in 168.398 CPU sec, 5.954 real sec
sorting block 9, #reads = 1079222 mem = 536880497 peak = 961601536
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281023, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 349, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (363.99, 159.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675100 sequences (100000270 bp) in 3.063 CPU sec, 3.470 real sec...
[M::process] read 674688 sequences (100000193 bp) in 2.369 CPU sec, 2.389 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281351, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 349, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (364.01, 160.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675078 reads in 256.238 CPU sec, 8.832 real sec
[M::mem_process_seqs] Processed 675114 reads in 163.140 CPU sec, 5.583 real sec
sorting block 10, #reads = 1079253 mem = 536878498 peak = 961601536
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281114, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 344, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (359.49, 157.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674568 sequences (100000129 bp) in 3.258 CPU sec, 3.567 real sec...
[M::process] read 674674 sequences (100000254 bp) in 2.368 CPU sec, 2.374 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281274, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 351, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (364.78, 157.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675100 reads in 255.349 CPU sec, 8.691 real sec
sorting block 11, #reads = 1078640 mem = 536881031 peak = 992141312
[M::mem_process_seqs] Processed 674688 reads in 161.712 CPU sec, 5.387 real sec
sorting block 12, #reads = 1080244 mem = 536877613 peak = 999739392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281098, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 353, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (366.77, 159.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674582 sequences (100000170 bp) in 2.902 CPU sec, 3.085 real sec...
[M::process] read 674468 sequences (100000065 bp) in 2.377 CPU sec, 2.380 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280872, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 348, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (361.70, 156.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674568 reads in 234.853 CPU sec, 7.624 real sec
[M::mem_process_seqs] Processed 674674 reads in 147.704 CPU sec, 4.735 real sec
sorting block 13, #reads = 1079055 mem = 536877705 peak = 999739392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280540, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 347, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (360.75, 155.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674790 sequences (100000112 bp) in 2.935 CPU sec, 3.048 real sec...
[M::process] read 674840 sequences (100000291 bp) in 2.220 CPU sec, 2.217 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281293, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 347, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (361.00, 155.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674582 reads in 211.370 CPU sec, 6.766 real sec
[M::mem_process_seqs] Processed 674468 reads in 137.146 CPU sec, 4.382 real sec
sorting block 14, #reads = 1078267 mem = 536878001 peak = 999739392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281114, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 343, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (357.19, 154.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674426 sequences (100000068 bp) in 2.891 CPU sec, 3.018 real sec...
[M::process] read 674556 sequences (100000127 bp) in 2.260 CPU sec, 2.261 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279914, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 345, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (359.02, 154.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674790 reads in 206.497 CPU sec, 6.614 real sec
sorting block 15, #reads = 1078715 mem = 536875624 peak = 999739392
[M::mem_process_seqs] Processed 674840 reads in 125.207 CPU sec, 3.976 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280743, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 346, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (359.32, 153.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674894 sequences (100000012 bp) in 3.306 CPU sec, 3.456 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280247, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 347, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (360.47, 155.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674934 sequences (100000063 bp) in 2.445 CPU sec, 2.448 real sec...
[M::mem_process_seqs] Processed 674426 reads in 208.409 CPU sec, 6.613 real sec
sorting block 16, #reads = 1077683 mem = 536878895 peak = 999739392
[M::mem_process_seqs] Processed 674556 reads in 140.134 CPU sec, 4.439 real sec
sorting block 17, #reads = 1076801 mem = 536882137 peak = 1038573568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280429, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 343, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (356.74, 153.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674798 sequences (100000173 bp) in 2.837 CPU sec, 2.930 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280639, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 344, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (358.12, 154.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (358, 737, 737)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1495)
[M::mem_pestat] mean and std.dev: (587.78, 265.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1874)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674706 sequences (100000187 bp) in 2.280 CPU sec, 2.272 real sec...
[M::mem_process_seqs] Processed 674894 reads in 190.386 CPU sec, 6.037 real sec
[M::mem_process_seqs] Processed 674934 reads in 119.431 CPU sec, 3.768 real sec
sorting block 18, #reads = 1077252 mem = 536883701 peak = 1038573568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280570, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 344, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (358.28, 154.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674804 sequences (100000003 bp) in 3.082 CPU sec, 3.184 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280651, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 345, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (359.03, 154.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674480 sequences (100000100 bp) in 1.820 CPU sec, 1.819 real sec...
[M::mem_process_seqs] Processed 674798 reads in 172.984 CPU sec, 5.478 real sec
[M::mem_process_seqs] Processed 674706 reads in 112.478 CPU sec, 3.570 real sec
sorting block 19, #reads = 1078467 mem = 536879949 peak = 1038573568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281140, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 345, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (359.08, 155.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674442 sequences (100000267 bp) in 2.842 CPU sec, 2.936 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280503, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 345, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (359.86, 155.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674550 sequences (100000177 bp) in 1.780 CPU sec, 1.779 real sec...
[M::mem_process_seqs] Processed 674804 reads in 170.304 CPU sec, 5.400 real sec
sorting block 20, #reads = 1078170 mem = 536879639 peak = 1038573568
[M::mem_process_seqs] Processed 674480 reads in 108.544 CPU sec, 3.437 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280800, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 349, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (363.91, 158.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674122 sequences (100000229 bp) in 2.584 CPU sec, 2.650 real sec...
[M::process] read 673902 sequences (100000238 bp) in 1.690 CPU sec, 1.698 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280919, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 350, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (363.84, 157.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674442 reads in 165.570 CPU sec, 5.254 real sec
sorting block 21, #reads = 1077021 mem = 536879353 peak = 1038573568
[M::mem_process_seqs] Processed 674550 reads in 109.246 CPU sec, 3.469 real sec
sorting block 22, #reads = 1075979 mem = 536879289 peak = 1061789696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280092, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 355, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (369.85, 161.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676102 sequences (100000021 bp) in 2.868 CPU sec, 2.936 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280425, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 353, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (368.42, 160.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674122 reads in 158.407 CPU sec, 5.031 real sec
[M::mem_process_seqs] Processed 673902 reads in 105.287 CPU sec, 3.314 real sec
[M::process] read 677740 sequences (100000171 bp) in 1.986 CPU sec, 2.017 real sec...
sorting block 23, #reads = 1077035 mem = 536879372 peak = 1061789696
[M::process] read 677106 sequences (100000177 bp) in 2.587 CPU sec, 2.657 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281775, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 329, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886)
[M::mem_pestat] mean and std.dev: (344.61, 154.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1106)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676820 sequences (100000123 bp) in 1.640 CPU sec, 1.640 real sec...
[M::mem_process_seqs] Processed 677740 reads in 87.727 CPU sec, 2.758 real sec
sorting block 24, #reads = 1079091 mem = 536880091 peak = 1061789696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281737, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 340, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (356.55, 158.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280942, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 334, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (349.44, 156.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676102 reads in 247.260 CPU sec, 7.845 real sec
[M::process] read 676816 sequences (100000024 bp) in 2.021 CPU sec, 2.021 real sec...
[M::mem_process_seqs] Processed 677106 reads in 114.873 CPU sec, 3.655 real sec
sorting block 25, #reads = 1079602 mem = 536880391 peak = 1061789696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281480, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 335, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (349.31, 155.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676322 sequences (100000002 bp) in 2.751 CPU sec, 2.875 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280698, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 335, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (349.31, 154.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1107)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676820 reads in 151.784 CPU sec, 4.825 real sec
[M::process] read 676302 sequences (100000051 bp) in 1.691 CPU sec, 1.712 real sec...
[M::mem_process_seqs] Processed 676816 reads in 100.746 CPU sec, 3.186 real sec
sorting block 26, #reads = 1082842 mem = 536878725 peak = 1061789696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280710, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 335, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881)
[M::mem_pestat] mean and std.dev: (349.46, 153.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676070 sequences (100000012 bp) in 2.910 CPU sec, 3.005 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281076, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 337, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (351.16, 153.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1099)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676322 reads in 149.000 CPU sec, 4.717 real sec
[M::mem_process_seqs] Processed 676302 reads in 94.389 CPU sec, 2.971 real sec
sorting block 27, #reads = 1080782 mem = 536878461 peak = 1061789696
[M::process] read 675984 sequences (100000110 bp) in 2.529 CPU sec, 2.549 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281055, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 338, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886)
[M::mem_pestat] mean and std.dev: (351.90, 153.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1103)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675798 sequences (100000005 bp) in 2.596 CPU sec, 2.658 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280860, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 340, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (353.69, 154.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676070 reads in 149.494 CPU sec, 4.753 real sec
sorting block 28, #reads = 1081818 mem = 536878703 peak = 1061789696
[M::mem_process_seqs] Processed 675984 reads in 95.846 CPU sec, 3.015 real sec
[M::process] read 675632 sequences (100000267 bp) in 2.603 CPU sec, 2.661 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281319, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 342, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (355.71, 155.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 29, #reads = 1081560 mem = 536878024 peak = 1061789696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281168, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 345, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (358.18, 156.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675704 sequences (100000281 bp) in 3.262 CPU sec, 3.508 real sec...
[M::mem_process_seqs] Processed 675798 reads in 146.591 CPU sec, 4.685 real sec
[M::mem_process_seqs] Processed 675632 reads in 95.695 CPU sec, 3.045 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281414, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 345, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (358.18, 156.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675704 reads in 80.236 CPU sec, 2.660 real sec
[M::process] read 675776 sequences (100000214 bp) in 3.084 CPU sec, 3.289 real sec...
sorting block 30, #reads = 1080842 mem = 536885366 peak = 1061789696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280938, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 346, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (359.05, 156.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675600 sequences (100000184 bp) in 2.427 CPU sec, 2.542 real sec...
[M::mem_process_seqs] Processed 675776 reads in 83.354 CPU sec, 2.735 real sec
sorting block 31, #reads = 1080190 mem = 536879951 peak = 1061789696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281122, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 345, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (359.01, 156.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675252 sequences (100000208 bp) in 2.476 CPU sec, 2.667 real sec...
[M::mem_process_seqs] Processed 675600 reads in 81.343 CPU sec, 2.675 real sec
sorting block 32, #reads = 1080533 mem = 536881267 peak = 1061789696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280894, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 347, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (359.44, 155.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675118 sequences (100000288 bp) in 2.267 CPU sec, 2.391 real sec...
[M::mem_process_seqs] Processed 675252 reads in 81.026 CPU sec, 2.624 real sec
[M::process] read 674930 sequences (100000156 bp) in 2.258 CPU sec, 2.318 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280558, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 349, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (362.08, 157.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675084 sequences (100000047 bp) in 1.400 CPU sec, 1.407 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280885, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 350, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (362.93, 157.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675162 sequences (100000026 bp) in 1.320 CPU sec, 1.319 real sec...
[M::mem_process_seqs] Processed 675118 reads in 149.364 CPU sec, 4.694 real sec
sorting block 33, #reads = 1080583 mem = 536879590 peak = 1061789696
[M::mem_process_seqs] Processed 674930 reads in 97.308 CPU sec, 3.071 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280451, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 34, #reads = 1079682 mem = 536878163 peak = 1114296320
[M::mem_pestat] (25, 50, 75) percentile: (243, 349, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (361.61, 156.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674678 sequences (100000221 bp) in 2.341 CPU sec, 2.498 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280609, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 348, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (360.31, 155.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674600 sequences (100000253 bp) in 1.460 CPU sec, 1.465 real sec...
[M::mem_process_seqs] Processed 675084 reads in 149.803 CPU sec, 4.756 real sec
[M::mem_process_seqs] Processed 675162 reads in 96.020 CPU sec, 3.045 real sec
sorting block 35, #reads = 1080681 mem = 536878543 peak = 1114296320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281244, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 353, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (365.46, 158.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674866 sequences (100000282 bp) in 2.284 CPU sec, 2.331 real sec...
[M::process] read 674832 sequences (100000236 bp) in 1.450 CPU sec, 1.451 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281408, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 351, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (364.47, 157.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674678 reads in 146.551 CPU sec, 4.641 real sec
[M::mem_process_seqs] Processed 674600 reads in 93.092 CPU sec, 2.957 real sec
sorting block 36, #reads = 1079089 mem = 536878592 peak = 1114296320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280820, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 343, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (357.00, 153.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674664 sequences (100000229 bp) in 2.527 CPU sec, 2.602 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281137, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 349, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (362.61, 157.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674520 sequences (100000132 bp) in 1.460 CPU sec, 1.467 real sec...
[M::mem_process_seqs] Processed 674866 reads in 147.987 CPU sec, 4.689 real sec
sorting block 37, #reads = 1079639 mem = 536878936 peak = 1114296320
[M::mem_process_seqs] Processed 674832 reads in 96.734 CPU sec, 3.064 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281033, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 350, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (363.66, 157.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674434 sequences (100000047 bp) in 2.619 CPU sec, 2.699 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280831, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 349, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (362.85, 156.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674604 sequences (100000099 bp) in 1.520 CPU sec, 1.518 real sec...
[M::mem_process_seqs] Processed 674664 reads in 150.829 CPU sec, 4.771 real sec
sorting block 38, #reads = 1078997 mem = 536880555 peak = 1114296320
[M::mem_process_seqs] Processed 674520 reads in 97.072 CPU sec, 3.075 real sec
sorting block 39, #reads = 1078831 mem = 536878216 peak = 1114296320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281060, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 349, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (362.78, 156.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675330 sequences (100000110 bp) in 2.187 CPU sec, 2.261 real sec...
[M::process] read 674610 sequences (100000039 bp) in 1.520 CPU sec, 1.521 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 281099, 2, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (455, 455, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (455, 455)
[M::mem_pestat] mean and std.dev: (455.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (455, 455)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 352, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (365.23, 157.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 674434 reads in 151.290 CPU sec, 4.800 real sec
[M::mem_process_seqs] Processed 674604 reads in 98.646 CPU sec, 3.138 real sec
sorting block 40, #reads = 1078402 mem = 536879237 peak = 1114296320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281537, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 341, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (356.40, 155.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674452 sequences (100000131 bp) in 2.391 CPU sec, 2.467 real sec...
[M::process] read 674678 sequences (100000238 bp) in 1.530 CPU sec, 1.527 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 280943, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 347, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (361.43, 156.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675330 reads in 154.195 CPU sec, 4.909 real sec
[M::mem_process_seqs] Processed 674610 reads in 100.972 CPU sec, 3.216 real sec
sorting block 41, #reads = 1079771 mem = 536879084 peak = 1114296320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281210, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 349, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (362.58, 156.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674834 sequences (100000028 bp) in 2.240 CPU sec, 2.345 real sec...
[M::process] read 674736 sequences (100000203 bp) in 1.540 CPU sec, 1.539 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281249, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 345, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (359.27, 155.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674452 reads in 148.411 CPU sec, 4.725 real sec
sorting block 42, #reads = 1078885 mem = 536879561 peak = 1114296320
[M::mem_process_seqs] Processed 674678 reads in 94.155 CPU sec, 2.989 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281192, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 344, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (358.20, 155.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674732 sequences (100000123 bp) in 2.935 CPU sec, 3.015 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280997, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 347, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (361.07, 157.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674834 reads in 142.644 CPU sec, 4.512 real sec
sorting block 43, #reads = 1077840 mem = 536878274 peak = 1114296320
[M::mem_process_seqs] Processed 674736 reads in 94.398 CPU sec, 2.971 real sec
[M::process] read 674766 sequences (100000296 bp) in 2.378 CPU sec, 2.441 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281178, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 344, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (358.55, 155.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 44, #reads = 1078837 mem = 536882289 peak = 1114296320
[M::process] read 674794 sequences (100000280 bp) in 2.444 CPU sec, 2.639 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280717, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 345, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (360.02, 156.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674732 reads in 143.386 CPU sec, 4.561 real sec
[M::mem_process_seqs] Processed 674766 reads in 95.386 CPU sec, 3.014 real sec
[M::process] read 674432 sequences (100000209 bp) in 2.258 CPU sec, 2.396 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280767, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 345, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (359.72, 156.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 45, #reads = 1077991 mem = 536877495 peak = 1114296320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280857, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 348, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (362.73, 157.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674568 sequences (100000266 bp) in 2.543 CPU sec, 2.655 real sec...
[M::mem_process_seqs] Processed 674794 reads in 143.348 CPU sec, 4.602 real sec
[M::mem_process_seqs] Processed 674432 reads in 97.319 CPU sec, 3.073 real sec
sorting block 46, #reads = 1077380 mem = 536878305 peak = 1114296320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281360, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 351, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (366.39, 160.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674344 sequences (100000290 bp) in 3.079 CPU sec, 3.207 real sec...
[M::mem_process_seqs] Processed 674568 reads in 80.389 CPU sec, 2.641 real sec
sorting block 47, #reads = 1077491 mem = 536878406 peak = 1114296320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281356, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 353, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (368.38, 161.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674344 reads in 78.041 CPU sec, 2.535 real sec
[M::process] read 674306 sequences (100000266 bp) in 3.152 CPU sec, 3.473 real sec...
sorting block 48, #reads = 1078348 mem = 536878270 peak = 1114296320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281084, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 355, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (370.36, 162.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674306 reads in 79.940 CPU sec, 2.580 real sec
[M::process] read 676330 sequences (100000205 bp) in 2.682 CPU sec, 2.794 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281944, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 340, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (355.10, 157.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677572 sequences (100000027 bp) in 2.405 CPU sec, 2.501 real sec...
[M::mem_process_seqs] Processed 676330 reads in 78.322 CPU sec, 2.519 real sec
sorting block 49, #reads = 1077765 mem = 536877614 peak = 1114296320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281961, 2, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 330, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879)
[M::mem_pestat] mean and std.dev: (345.20, 153.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1096)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (278, 495, 707)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1565)
[M::mem_pestat] mean and std.dev: (483.27, 196.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1994)
[M::mem_pestat] skip orientation RR
[M::process] read 677332 sequences (100000131 bp) in 2.021 CPU sec, 2.110 real sec...
[M::mem_process_seqs] Processed 677572 reads in 75.820 CPU sec, 2.442 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282107, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 333, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (348.13, 155.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676770 sequences (100000197 bp) in 2.612 CPU sec, 2.687 real sec...
[M::mem_process_seqs] Processed 677332 reads in 74.850 CPU sec, 2.420 real sec
sorting block 50, #reads = 1080990 mem = 536878679 peak = 1114296320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281588, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 334, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879)
[M::mem_pestat] mean and std.dev: (347.89, 153.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1095)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676770 reads in 75.359 CPU sec, 2.438 real sec
[M::process] read 676472 sequences (100000258 bp) in 3.084 CPU sec, 3.377 real sec...
sorting block 51, #reads = 1082292 mem = 536880278 peak = 1114296320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281679, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 338, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (351.53, 154.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1106)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676158 sequences (100000128 bp) in 2.045 CPU sec, 2.100 real sec...
[M::mem_process_seqs] Processed 676472 reads in 75.097 CPU sec, 2.416 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282087, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 337, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (350.92, 153.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676172 sequences (100000229 bp) in 2.175 CPU sec, 2.260 real sec...
[M::mem_process_seqs] Processed 676158 reads in 73.641 CPU sec, 2.367 real sec
sorting block 52, #reads = 1080549 mem = 536880650 peak = 1114296320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281584, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 338, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (351.99, 154.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1107)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676024 sequences (100000257 bp) in 2.360 CPU sec, 2.403 real sec...
[M::mem_process_seqs] Processed 676172 reads in 76.182 CPU sec, 2.448 real sec
sorting block 53, #reads = 1080863 mem = 536880839 peak = 1114296320
[M::process] read 675836 sequences (100000102 bp) in 2.062 CPU sec, 2.136 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281060, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 340, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (353.41, 154.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675404 sequences (100000236 bp) in 1.370 CPU sec, 1.368 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281894, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 343, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (356.58, 156.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676024 reads in 135.910 CPU sec, 4.269 real sec
[M::process] read 675624 sequences (100000012 bp) in 1.299 CPU sec, 1.298 real sec...
[M::mem_process_seqs] Processed 675836 reads in 88.804 CPU sec, 2.797 real sec
sorting block 54, #reads = 1080397 mem = 536876891 peak = 1114296320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281115, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 344, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (357.11, 155.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675436 sequences (100000170 bp) in 2.295 CPU sec, 2.413 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281301, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 343, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (357.12, 155.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675278 sequences (100000053 bp) in 1.460 CPU sec, 1.464 real sec...
[M::mem_process_seqs] Processed 675404 reads in 134.523 CPU sec, 4.261 real sec
[M::mem_process_seqs] Processed 675624 reads in 89.235 CPU sec, 2.826 real sec
sorting block 55, #reads = 1079466 mem = 536880138 peak = 1114296320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281991, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 348, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (361.80, 158.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675230 sequences (100000238 bp) in 1.874 CPU sec, 1.939 real sec...
[M::process] read 674960 sequences (100000183 bp) in 1.380 CPU sec, 1.373 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281637, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 349, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (361.51, 157.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675436 reads in 132.650 CPU sec, 4.203 real sec
[M::mem_process_seqs] Processed 675278 reads in 85.120 CPU sec, 2.713 real sec
sorting block 56, #reads = 1079828 mem = 536880806 peak = 1114984448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281473, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 351, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (365.45, 160.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674612 sequences (100000203 bp) in 2.561 CPU sec, 2.765 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281034, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 350, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (364.49, 158.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675230 reads in 132.518 CPU sec, 4.207 real sec
[M::mem_process_seqs] Processed 674960 reads in 86.910 CPU sec, 2.740 real sec
sorting block 57, #reads = 1080776 mem = 536876781 peak = 1114984448
[M::process] read 674782 sequences (100000019 bp) in 1.936 CPU sec, 2.025 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281234, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 351, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (364.51, 158.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 58, #reads = 1079956 mem = 536880336 peak = 1124360192
[M::process] read 674798 sequences (100000223 bp) in 2.227 CPU sec, 2.348 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281091, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 352, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (365.85, 159.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674612 reads in 138.408 CPU sec, 4.404 real sec
[M::process] read 674610 sequences (100000140 bp) in 1.557 CPU sec, 1.567 real sec...
[M::mem_process_seqs] Processed 674782 reads in 90.548 CPU sec, 2.855 real sec
sorting block 59, #reads = 1079509 mem = 536879799 peak = 1124360192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281197, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 351, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (365.14, 159.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674440 sequences (100000154 bp) in 2.198 CPU sec, 2.291 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281218, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 352, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (365.56, 158.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674422 sequences (100000088 bp) in 1.440 CPU sec, 1.432 real sec...
[M::mem_process_seqs] Processed 674798 reads in 137.929 CPU sec, 4.376 real sec
[M::process] read 674288 sequences (100000248 bp) in 1.728 CPU sec, 1.733 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280660, 3, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 352, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (365.99, 158.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (341, 2396, 2551)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6971)
[M::mem_pestat] mean and std.dev: (1831.54, 1003.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9181)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 674440 reads in 76.027 CPU sec, 2.408 real sec
sorting block 60, #reads = 1079187 mem = 536878804 peak = 1124360192
[M::mem_process_seqs] Processed 674610 reads in 189.216 CPU sec, 6.011 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280101, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 352, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (366.74, 159.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 61, #reads = 1079161 mem = 536878769 peak = 1149194240
[M::process] read 674586 sequences (100000134 bp) in 2.181 CPU sec, 2.283 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280922, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 353, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (366.53, 159.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674422 reads in 153.814 CPU sec, 4.888 real sec
[M::process] read 674408 sequences (100000105 bp) in 1.560 CPU sec, 1.569 real sec...
[M::mem_process_seqs] Processed 674288 reads in 92.059 CPU sec, 2.907 real sec
sorting block 62, #reads = 1078732 mem = 536880872 peak = 1149194240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281220, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 346, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (360.12, 155.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674568 sequences (100000227 bp) in 1.884 CPU sec, 1.988 real sec...
[M::process] read 674596 sequences (100000040 bp) in 1.500 CPU sec, 1.499 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281280, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 348, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (363.92, 158.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674586 reads in 136.610 CPU sec, 4.338 real sec
[M::mem_process_seqs] Processed 674408 reads in 88.884 CPU sec, 2.813 real sec
sorting block 63, #reads = 1080299 mem = 536879354 peak = 1149194240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280685, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 347, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (362.25, 158.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674458 sequences (100000284 bp) in 2.212 CPU sec, 2.292 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280755, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 346, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (361.87, 158.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674810 sequences (100000155 bp) in 1.500 CPU sec, 1.502 real sec...
[M::mem_process_seqs] Processed 674568 reads in 132.645 CPU sec, 4.204 real sec
sorting block 64, #reads = 1080460 mem = 536877532 peak = 1149194240
[M::mem_process_seqs] Processed 674596 reads in 86.246 CPU sec, 2.728 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280675, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 344, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (359.64, 157.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674974 sequences (100000247 bp) in 2.389 CPU sec, 2.637 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280675, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 345, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (360.47, 158.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674458 reads in 136.431 CPU sec, 4.332 real sec
sorting block 65, #reads = 1079180 mem = 536879269 peak = 1149194240
[M::process] read 674618 sequences (100000273 bp) in 1.616 CPU sec, 1.630 real sec...
[M::mem_process_seqs] Processed 674810 reads in 88.910 CPU sec, 2.805 real sec
sorting block 66, #reads = 1078661 mem = 536884582 peak = 1156362240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281170, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 342, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (358.26, 157.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674966 sequences (100000023 bp) in 2.131 CPU sec, 2.234 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280857, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 341, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (357.22, 155.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674884 sequences (100000250 bp) in 1.500 CPU sec, 1.497 real sec...
[M::mem_process_seqs] Processed 674974 reads in 135.697 CPU sec, 4.308 real sec
[M::mem_process_seqs] Processed 674618 reads in 88.961 CPU sec, 2.818 real sec
sorting block 67, #reads = 1079204 mem = 536880797 peak = 1156362240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280908, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 341, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (356.88, 156.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674938 sequences (100000205 bp) in 1.991 CPU sec, 2.083 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280904, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 342, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (357.17, 155.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674788 sequences (100000214 bp) in 1.520 CPU sec, 1.518 real sec...
[M::mem_process_seqs] Processed 674966 reads in 139.640 CPU sec, 4.439 real sec
[M::mem_process_seqs] Processed 674884 reads in 89.503 CPU sec, 2.845 real sec
sorting block 68, #reads = 1077714 mem = 536878313 peak = 1156362240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280802, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 340, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (356.58, 155.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1111)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674620 sequences (100000033 bp) in 2.484 CPU sec, 2.725 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281262, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 341, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (356.59, 155.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1111)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674938 reads in 139.829 CPU sec, 4.437 real sec
[M::process] read 674704 sequences (100000265 bp) in 1.546 CPU sec, 1.553 real sec...
[M::mem_process_seqs] Processed 674788 reads in 90.637 CPU sec, 2.870 real sec
sorting block 69, #reads = 1079349 mem = 536881660 peak = 1156362240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281482, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 343, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (359.65, 157.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674470 sequences (100000089 bp) in 2.360 CPU sec, 2.599 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281324, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 346, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (361.85, 158.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674620 reads in 139.378 CPU sec, 4.433 real sec
sorting block 70, #reads = 1078274 mem = 536879039 peak = 1156362240
[M::process] read 674470 sequences (100000040 bp) in 1.716 CPU sec, 1.748 real sec...
[M::mem_process_seqs] Processed 674704 reads in 90.311 CPU sec, 2.855 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281245, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 348, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (363.69, 159.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 71, #reads = 1079858 mem = 536877837 peak = 1191809024
[M::process] read 674246 sequences (100000135 bp) in 2.567 CPU sec, 2.823 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280984, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 351, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (366.69, 161.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674470 reads in 138.192 CPU sec, 4.413 real sec
[M::mem_process_seqs] Processed 674470 reads in 91.708 CPU sec, 2.905 real sec
[M::process] read 674112 sequences (100000132 bp) in 1.845 CPU sec, 1.904 real sec...
sorting block 72, #reads = 1079412 mem = 536878657 peak = 1191809024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280873, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 350, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (366.36, 160.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674058 sequences (100000261 bp) in 2.304 CPU sec, 2.555 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281001, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 358, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (373.70, 165.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674246 reads in 138.056 CPU sec, 4.399 real sec
[M::mem_process_seqs] Processed 674112 reads in 89.841 CPU sec, 2.844 real sec
[M::process] read 677500 sequences (100000120 bp) in 1.796 CPU sec, 1.840 real sec...
sorting block 73, #reads = 1078345 mem = 536877706 peak = 1191809024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280639, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 356, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (372.19, 164.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677092 sequences (100000058 bp) in 2.145 CPU sec, 2.253 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281734, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 330, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (345.77, 154.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1100)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676758 sequences (100000232 bp) in 1.460 CPU sec, 1.459 real sec...
[M::mem_process_seqs] Processed 674058 reads in 144.079 CPU sec, 4.584 real sec
sorting block 74, #reads = 1078945 mem = 536879721 peak = 1191809024
[M::mem_process_seqs] Processed 677500 reads in 93.519 CPU sec, 2.969 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281617, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 333, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (349.83, 157.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 75, #reads = 1077919 mem = 536878436 peak = 1201766400
[M::process] read 676432 sequences (100000217 bp) in 2.004 CPU sec, 2.099 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281436, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 335, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (350.28, 155.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676180 sequences (100000186 bp) in 1.480 CPU sec, 1.484 real sec...
[M::mem_process_seqs] Processed 677092 reads in 139.077 CPU sec, 4.423 real sec
[M::mem_process_seqs] Processed 676758 reads in 89.382 CPU sec, 2.839 real sec
sorting block 76, #reads = 1081294 mem = 536878273 peak = 1201766400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281806, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 341, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (357.98, 160.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675906 sequences (100000291 bp) in 2.334 CPU sec, 2.500 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281091, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 344, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (359.44, 160.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675558 sequences (100000044 bp) in 1.520 CPU sec, 1.518 real sec...
[M::mem_process_seqs] Processed 676432 reads in 137.921 CPU sec, 4.375 real sec
[M::mem_process_seqs] Processed 676180 reads in 89.400 CPU sec, 2.829 real sec
sorting block 77, #reads = 1081915 mem = 536879077 peak = 1201766400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281289, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 343, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (357.61, 158.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675532 sequences (100000062 bp) in 2.338 CPU sec, 2.597 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281053, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 343, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (358.39, 158.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675906 reads in 136.142 CPU sec, 4.348 real sec
[M::mem_process_seqs] Processed 675558 reads in 88.919 CPU sec, 2.819 real sec
[M::process] read 675384 sequences (100000164 bp) in 1.813 CPU sec, 1.872 real sec...
sorting block 78, #reads = 1081475 mem = 536879894 peak = 1201766400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281690, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 341, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (356.44, 156.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675210 sequences (100000136 bp) in 2.424 CPU sec, 2.690 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280640, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 345, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (359.91, 158.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675532 reads in 137.826 CPU sec, 4.389 real sec
[M::mem_process_seqs] Processed 675384 reads in 90.059 CPU sec, 2.850 real sec
sorting block 79, #reads = 1081505 mem = 536879189 peak = 1201766400
[M::process] read 675126 sequences (100000010 bp) in 1.711 CPU sec, 1.745 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281721, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 349, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (363.55, 159.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 80, #reads = 1080678 mem = 536878428 peak = 1210847232
[M::process] read 675136 sequences (100000156 bp) in 1.980 CPU sec, 2.076 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281259, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 351, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (364.80, 160.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674958 sequences (100000274 bp) in 1.510 CPU sec, 1.513 real sec...
[M::mem_process_seqs] Processed 675210 reads in 138.341 CPU sec, 4.400 real sec
[M::mem_process_seqs] Processed 675126 reads in 89.267 CPU sec, 2.828 real sec
sorting block 81, #reads = 1080854 mem = 536878392 peak = 1210847232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281270, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 351, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (364.95, 160.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674938 sequences (100000002 bp) in 2.242 CPU sec, 2.348 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281000, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 353, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (367.95, 162.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674760 sequences (100000118 bp) in 1.530 CPU sec, 1.531 real sec...
[M::mem_process_seqs] Processed 675136 reads in 140.327 CPU sec, 4.457 real sec
[M::mem_process_seqs] Processed 674958 reads in 91.025 CPU sec, 2.888 real sec
sorting block 82, #reads = 1081324 mem = 536878000 peak = 1210847232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280611, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 353, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (367.22, 162.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674690 sequences (100000249 bp) in 2.391 CPU sec, 2.634 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280436, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 355, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (369.17, 162.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674938 reads in 143.540 CPU sec, 4.568 real sec
[M::process] read 674550 sequences (100000113 bp) in 1.669 CPU sec, 1.697 real sec...
[M::mem_process_seqs] Processed 674760 reads in 93.244 CPU sec, 2.952 real sec
sorting block 83, #reads = 1080981 mem = 536881686 peak = 1210847232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280648, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 353, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (367.44, 160.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674768 sequences (100000229 bp) in 2.270 CPU sec, 2.368 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281057, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 354, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (367.57, 160.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674678 sequences (100000021 bp) in 1.560 CPU sec, 1.561 real sec...
[M::mem_process_seqs] Processed 674690 reads in 139.834 CPU sec, 4.444 real sec
sorting block 84, #reads = 1080854 mem = 536876536 peak = 1210847232
[M::mem_process_seqs] Processed 674550 reads in 89.924 CPU sec, 2.850 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281335, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 353, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (367.14, 160.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 85, #reads = 1080188 mem = 536878857 peak = 1216344064
[M::process] read 674598 sequences (100000020 bp) in 2.070 CPU sec, 2.174 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280868, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 352, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (365.59, 159.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674244 sequences (100000281 bp) in 1.610 CPU sec, 1.609 real sec...
[M::mem_process_seqs] Processed 674768 reads in 141.264 CPU sec, 4.490 real sec
[M::mem_process_seqs] Processed 674678 reads in 90.906 CPU sec, 2.886 real sec
sorting block 86, #reads = 1079134 mem = 536881415 peak = 1216344064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281600, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 352, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (365.84, 158.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673938 sequences (100000174 bp) in 2.286 CPU sec, 2.380 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280269, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 352, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (365.72, 158.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674052 sequences (100000162 bp) in 1.570 CPU sec, 1.575 real sec...
[M::mem_process_seqs] Processed 674598 reads in 141.742 CPU sec, 4.500 real sec
[M::mem_process_seqs] Processed 674244 reads in 91.337 CPU sec, 2.897 real sec
sorting block 87, #reads = 1079612 mem = 536880998 peak = 1216344064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280651, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 352, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (366.14, 158.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675132 sequences (100000031 bp) in 2.461 CPU sec, 2.712 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280694, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 353, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (367.20, 159.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673938 reads in 141.765 CPU sec, 4.512 real sec
[M::process] read 675210 sequences (100000301 bp) in 1.689 CPU sec, 1.706 real sec...
[M::mem_process_seqs] Processed 674052 reads in 91.589 CPU sec, 2.897 real sec
sorting block 88, #reads = 1079586 mem = 536880732 peak = 1216344064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280665, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 343, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (358.69, 157.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675346 sequences (100000205 bp) in 2.024 CPU sec, 2.111 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280575, 8, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 341, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (356.46, 156.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675098 sequences (100000047 bp) in 1.570 CPU sec, 1.568 real sec...
[M::mem_process_seqs] Processed 675132 reads in 143.921 CPU sec, 4.587 real sec
sorting block 89, #reads = 1079317 mem = 536883409 peak = 1216344064
[M::mem_process_seqs] Processed 675210 reads in 92.395 CPU sec, 2.927 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281433, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 338, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (353.30, 153.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1101)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 90, #reads = 1080477 mem = 536878852 peak = 1216344064
[M::process] read 675040 sequences (100000071 bp) in 2.117 CPU sec, 2.208 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281307, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 343, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (358.71, 157.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674736 sequences (100000013 bp) in 1.570 CPU sec, 1.570 real sec...
[M::mem_process_seqs] Processed 675346 reads in 143.766 CPU sec, 4.571 real sec
[M::mem_process_seqs] Processed 675098 reads in 92.744 CPU sec, 2.943 real sec
sorting block 91, #reads = 1079065 mem = 536878594 peak = 1216344064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281168, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 343, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (357.59, 155.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675040 sequences (100000114 bp) in 2.253 CPU sec, 2.347 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281145, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 342, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (357.32, 155.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675152 sequences (100000234 bp) in 1.600 CPU sec, 1.595 real sec...
[M::mem_process_seqs] Processed 675040 reads in 143.572 CPU sec, 4.561 real sec
[M::mem_process_seqs] Processed 674736 reads in 92.732 CPU sec, 2.938 real sec
sorting block 92, #reads = 1079082 mem = 536879068 peak = 1216344064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281061, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 340, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (355.88, 154.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674878 sequences (100000196 bp) in 2.095 CPU sec, 2.185 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281100, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 339, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (354.66, 154.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675002 sequences (100000119 bp) in 1.570 CPU sec, 1.567 real sec...
[M::mem_process_seqs] Processed 675040 reads in 142.275 CPU sec, 4.530 real sec
[M::mem_process_seqs] Processed 675152 reads in 92.546 CPU sec, 2.929 real sec
sorting block 93, #reads = 1078613 mem = 536878913 peak = 1216344064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281149, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 340, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (355.39, 154.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674640 sequences (100000222 bp) in 2.321 CPU sec, 2.416 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280516, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 342, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (357.14, 155.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674716 sequences (100000174 bp) in 1.560 CPU sec, 1.561 real sec...
[M::mem_process_seqs] Processed 674878 reads in 142.549 CPU sec, 4.528 real sec
sorting block 94, #reads = 1080000 mem = 536878859 peak = 1216344064
[M::mem_process_seqs] Processed 675002 reads in 91.407 CPU sec, 2.900 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280976, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 346, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (360.76, 156.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 95, #reads = 1078838 mem = 536878217 peak = 1216344064
[M::process] read 674842 sequences (100000117 bp) in 2.495 CPU sec, 2.709 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280690, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 344, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (359.31, 155.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674640 reads in 144.890 CPU sec, 4.625 real sec
[M::mem_process_seqs] Processed 674716 reads in 94.127 CPU sec, 2.978 real sec
[M::process] read 674492 sequences (100000090 bp) in 1.766 CPU sec, 1.795 real sec...
sorting block 96, #reads = 1078751 mem = 536878114 peak = 1216344064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281389, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 345, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (360.28, 157.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673710 sequences (100000054 bp) in 2.763 CPU sec, 3.011 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280562, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 349, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (364.40, 159.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674842 reads in 146.530 CPU sec, 4.669 real sec
[M::mem_process_seqs] Processed 674492 reads in 95.915 CPU sec, 3.039 real sec
[M::process] read 673742 sequences (100000117 bp) in 1.834 CPU sec, 1.873 real sec...
sorting block 97, #reads = 1079057 mem = 536879584 peak = 1216344064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280296, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 356, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (370.43, 160.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674390 sequences (100000157 bp) in 2.537 CPU sec, 2.793 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281483, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 357, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (372.43, 161.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673710 reads in 141.221 CPU sec, 4.507 real sec
[M::mem_process_seqs] Processed 673742 reads in 91.668 CPU sec, 2.907 real sec
sorting block 98, #reads = 1078377 mem = 536878083 peak = 1216344064
[M::process] read 674292 sequences (100000282 bp) in 2.301 CPU sec, 2.450 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280898, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 351, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (365.93, 160.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681372 sequences (100000171 bp) in 2.583 CPU sec, 2.790 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280948, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 354, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (369.75, 163.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674390 reads in 143.612 CPU sec, 4.601 real sec
[M::mem_process_seqs] Processed 674292 reads in 94.007 CPU sec, 2.984 real sec
sorting block 99, #reads = 1078991 mem = 536880557 peak = 1216344064
[M::process] read 680516 sequences (100000208 bp) in 2.483 CPU sec, 2.679 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283938, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 308, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (329.16, 154.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 100, #reads = 1078524 mem = 536876304 peak = 1216344064
[M::process] read 679652 sequences (100000221 bp) in 2.424 CPU sec, 2.646 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283089, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 313, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (332.70, 154.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681372 reads in 164.261 CPU sec, 5.258 real sec
[M::mem_process_seqs] Processed 680516 reads in 105.110 CPU sec, 3.331 real sec
[M::process] read 679150 sequences (100000152 bp) in 2.168 CPU sec, 2.304 real sec...
sorting block 101, #reads = 1077763 mem = 536877530 peak = 1216344064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282749, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 319, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (336.99, 154.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678876 sequences (100000099 bp) in 2.242 CPU sec, 2.324 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283109, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 323, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (340.19, 155.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678280 sequences (100000267 bp) in 1.500 CPU sec, 1.497 real sec...
[M::mem_process_seqs] Processed 679652 reads in 161.718 CPU sec, 5.151 real sec
sorting block 102, #reads = 1077432 mem = 536878327 peak = 1216344064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282544, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 326, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (342.50, 155.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677952 sequences (100000109 bp) in 1.754 CPU sec, 1.754 real sec...
[M::mem_process_seqs] Processed 678876 reads in 86.300 CPU sec, 2.742 real sec
[M::mem_process_seqs] Processed 679150 reads in 212.345 CPU sec, 6.748 real sec
sorting block 103, #reads = 1078023 mem = 536880768 peak = 1216344064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281948, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 329, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (345.87, 156.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677268 sequences (100000200 bp) in 2.427 CPU sec, 2.553 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282289, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 331, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (347.58, 156.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677058 sequences (100000073 bp) in 1.590 CPU sec, 1.593 real sec...
[M::mem_process_seqs] Processed 678280 reads in 170.154 CPU sec, 5.431 real sec
[M::mem_process_seqs] Processed 677952 reads in 100.322 CPU sec, 3.211 real sec
sorting block 104, #reads = 1078538 mem = 536881153 peak = 1216344064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282009, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 337, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (352.15, 157.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676234 sequences (100000125 bp) in 2.332 CPU sec, 2.453 real sec...
[M::process] read 676260 sequences (100000259 bp) in 1.580 CPU sec, 1.576 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281782, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 341, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (356.11, 159.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677268 reads in 152.974 CPU sec, 4.867 real sec
[M::mem_process_seqs] Processed 677058 reads in 96.883 CPU sec, 3.080 real sec
sorting block 105, #reads = 1077986 mem = 536880238 peak = 1216344064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281414, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 347, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (360.88, 160.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676462 sequences (100000286 bp) in 2.601 CPU sec, 2.813 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281360, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 348, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (362.68, 161.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676234 reads in 151.831 CPU sec, 4.826 real sec
[M::process] read 676328 sequences (100000159 bp) in 1.630 CPU sec, 1.635 real sec...
sorting block 106, #reads = 1078274 mem = 536876122 peak = 1216344064
[M::mem_process_seqs] Processed 676260 reads in 97.545 CPU sec, 3.090 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281567, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 346, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (360.29, 160.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 107, #reads = 1078132 mem = 536879689 peak = 1216344064
[M::process] read 676242 sequences (100000096 bp) in 2.863 CPU sec, 3.093 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281022, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 347, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (360.50, 160.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676462 reads in 155.147 CPU sec, 4.944 real sec
[M::process] read 676292 sequences (100000028 bp) in 1.768 CPU sec, 1.780 real sec...
[M::mem_process_seqs] Processed 676328 reads in 100.328 CPU sec, 3.179 real sec
sorting block 108, #reads = 1077549 mem = 536880979 peak = 1216344064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281748, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 346, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (358.96, 158.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676272 sequences (100000248 bp) in 2.251 CPU sec, 2.358 real sec...
[M::process] read 676240 sequences (100000109 bp) in 1.630 CPU sec, 1.625 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282267, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 344, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (357.39, 157.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676242 reads in 161.455 CPU sec, 5.142 real sec
[M::mem_process_seqs] Processed 676292 reads in 101.696 CPU sec, 3.236 real sec
sorting block 109, #reads = 1074136 mem = 536881110 peak = 1216344064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281727, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 346, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (358.66, 158.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676042 sequences (100000029 bp) in 2.390 CPU sec, 2.493 real sec...
[M::process] read 676228 sequences (100000109 bp) in 1.610 CPU sec, 1.618 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281647, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 346, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (358.59, 157.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676272 reads in 161.403 CPU sec, 5.135 real sec
[M::mem_process_seqs] Processed 676240 reads in 101.796 CPU sec, 3.234 real sec
sorting block 110, #reads = 1074270 mem = 536877915 peak = 1230778368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282102, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 347, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (359.49, 157.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676418 sequences (100000031 bp) in 2.335 CPU sec, 2.426 real sec...
[M::process] read 676002 sequences (100000176 bp) in 1.660 CPU sec, 1.657 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282092, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 344, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (357.02, 156.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676042 reads in 156.319 CPU sec, 4.972 real sec
sorting block 111, #reads = 1075782 mem = 536880155 peak = 1258287104
[M::mem_process_seqs] Processed 676228 reads in 98.816 CPU sec, 3.136 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281557, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 342, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (355.94, 157.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 112, #reads = 1076170 mem = 536879342 peak = 1258749952
[M::process] read 675560 sequences (100000016 bp) in 2.886 CPU sec, 3.079 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281440, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 346, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (359.46, 158.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676418 reads in 160.221 CPU sec, 5.092 real sec
[M::process] read 675452 sequences (100000074 bp) in 2.029 CPU sec, 2.049 real sec...
[M::mem_process_seqs] Processed 676002 reads in 102.085 CPU sec, 3.223 real sec
sorting block 113, #reads = 1075869 mem = 536879616 peak = 1258749952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281810, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 348, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (360.46, 156.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675434 sequences (100000139 bp) in 2.496 CPU sec, 2.585 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281184, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 348, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (359.97, 155.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675180 sequences (100000170 bp) in 1.668 CPU sec, 1.677 real sec...
[M::mem_process_seqs] Processed 675560 reads in 159.289 CPU sec, 5.071 real sec
[M::mem_process_seqs] Processed 675452 reads in 102.180 CPU sec, 3.239 real sec
sorting block 114, #reads = 1076467 mem = 536878880 peak = 1258749952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281963, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 350, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (362.45, 157.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674986 sequences (100000031 bp) in 2.832 CPU sec, 3.043 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281422, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 353, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (364.92, 158.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675434 reads in 161.192 CPU sec, 5.122 real sec
[M::process] read 675368 sequences (100000185 bp) in 1.930 CPU sec, 1.936 real sec...
[M::mem_process_seqs] Processed 675180 reads in 104.011 CPU sec, 3.287 real sec
sorting block 115, #reads = 1075892 mem = 536878867 peak = 1258749952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281439, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 351, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (364.43, 158.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675138 sequences (100000060 bp) in 2.696 CPU sec, 2.891 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280938, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 348, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (361.39, 157.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674986 reads in 163.190 CPU sec, 5.188 real sec
[M::process] read 674962 sequences (100000170 bp) in 1.945 CPU sec, 1.960 real sec...
sorting block 116, #reads = 1074626 mem = 536878158 peak = 1258749952
[M::mem_process_seqs] Processed 675368 reads in 105.001 CPU sec, 3.318 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281548, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 349, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (361.97, 157.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 117, #reads = 1075346 mem = 536877611 peak = 1264685056
[M::process] read 674710 sequences (100000275 bp) in 2.818 CPU sec, 3.001 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281541, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 349, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (362.55, 157.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675138 reads in 162.047 CPU sec, 5.154 real sec
[M::process] read 674568 sequences (100000249 bp) in 1.941 CPU sec, 1.961 real sec...
[M::mem_process_seqs] Processed 674962 reads in 104.395 CPU sec, 3.301 real sec
sorting block 118, #reads = 1074654 mem = 536878335 peak = 1264685056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280852, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 352, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (364.45, 157.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675018 sequences (100000119 bp) in 2.529 CPU sec, 2.705 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280676, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 354, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (367.22, 159.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674710 reads in 160.077 CPU sec, 5.099 real sec
[M::process] read 674686 sequences (100000058 bp) in 1.827 CPU sec, 1.832 real sec...
[M::mem_process_seqs] Processed 674568 reads in 103.279 CPU sec, 3.269 real sec
sorting block 119, #reads = 1074003 mem = 536878584 peak = 1264685056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281359, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 352, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (365.84, 159.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674420 sequences (100000086 bp) in 2.589 CPU sec, 2.817 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281101, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 355, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (368.13, 160.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674312 sequences (100000119 bp) in 1.660 CPU sec, 1.667 real sec...
[M::mem_process_seqs] Processed 675018 reads in 159.849 CPU sec, 5.082 real sec
[M::mem_process_seqs] Processed 674686 reads in 101.721 CPU sec, 3.221 real sec
sorting block 120, #reads = 1074227 mem = 536881296 peak = 1264685056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281073, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 357, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (372.01, 163.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677372 sequences (100000002 bp) in 2.708 CPU sec, 2.927 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280643, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 357, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (372.08, 163.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674420 reads in 156.090 CPU sec, 4.965 real sec
sorting block 121, #reads = 1075925 mem = 536878313 peak = 1264685056
[M::process] read 683532 sequences (100000017 bp) in 1.896 CPU sec, 1.908 real sec...
[M::mem_process_seqs] Processed 674312 reads in 101.683 CPU sec, 3.215 real sec
sorting block 122, #reads = 1075152 mem = 536881435 peak = 1264914432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282074, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 337, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (353.71, 160.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681160 sequences (100000055 bp) in 2.423 CPU sec, 2.510 real sec...
[M::process] read 680462 sequences (100000037 bp) in 1.670 CPU sec, 1.671 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284760, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 293, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (311.93, 144.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677372 reads in 172.277 CPU sec, 5.485 real sec
[M::mem_process_seqs] Processed 683532 reads in 113.287 CPU sec, 3.602 real sec
sorting block 123, #reads = 1074051 mem = 536879138 peak = 1264914432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283838, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 306, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (323.72, 147.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679966 sequences (100000283 bp) in 2.300 CPU sec, 2.396 real sec...
[M::process] read 679830 sequences (100000077 bp) in 1.760 CPU sec, 1.760 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283340, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 312, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860)
[M::mem_pestat] mean and std.dev: (328.30, 148.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681160 reads in 171.554 CPU sec, 5.454 real sec
[M::mem_process_seqs] Processed 680462 reads in 108.421 CPU sec, 3.449 real sec
sorting block 124, #reads = 1073314 mem = 536878921 peak = 1264914432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283192, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 316, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865)
[M::mem_pestat] mean and std.dev: (331.08, 149.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1082)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679626 sequences (100000095 bp) in 2.748 CPU sec, 2.955 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283027, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 317, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864)
[M::mem_pestat] mean and std.dev: (331.77, 148.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1080)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679802 sequences (100000251 bp) in 1.660 CPU sec, 1.662 real sec...
[M::mem_process_seqs] Processed 679966 reads in 171.938 CPU sec, 5.466 real sec
sorting block 125, #reads = 1073207 mem = 536878009 peak = 1264914432
[M::mem_process_seqs] Processed 679830 reads in 110.633 CPU sec, 3.510 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283423, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 317, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (330.37, 146.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 126, #reads = 1073201 mem = 536876656 peak = 1264914432
[M::process] read 679100 sequences (100000157 bp) in 2.815 CPU sec, 2.981 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283272, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 316, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (329.05, 145.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1067)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679030 sequences (100000011 bp) in 1.980 CPU sec, 1.972 real sec...
[M::mem_process_seqs] Processed 679626 reads in 172.878 CPU sec, 5.485 real sec
[M::mem_process_seqs] Processed 679802 reads in 111.962 CPU sec, 3.544 real sec
sorting block 127, #reads = 1072257 mem = 536878509 peak = 1264914432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283071, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 322, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (335.39, 149.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1087)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678240 sequences (100000192 bp) in 2.853 CPU sec, 3.031 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283220, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 322, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (334.70, 147.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1087)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678048 sequences (100000043 bp) in 1.750 CPU sec, 1.749 real sec...
[M::mem_process_seqs] Processed 679100 reads in 169.015 CPU sec, 5.367 real sec
[M::mem_process_seqs] Processed 679030 reads in 108.170 CPU sec, 3.427 real sec
sorting block 128, #reads = 1072627 mem = 536879541 peak = 1264914432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282450, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 327, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (339.96, 150.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1106)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678208 sequences (100000227 bp) in 2.733 CPU sec, 2.938 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283252, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 330, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (342.70, 151.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677174 sequences (100000123 bp) in 1.699 CPU sec, 1.699 real sec...
[M::mem_process_seqs] Processed 678240 reads in 164.414 CPU sec, 5.225 real sec
[M::mem_process_seqs] Processed 678048 reads in 104.943 CPU sec, 3.326 real sec
sorting block 129, #reads = 1072284 mem = 536879500 peak = 1264914432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283192, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 329, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (342.39, 152.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677498 sequences (100000157 bp) in 2.595 CPU sec, 2.815 real sec...
[M::process] read 676894 sequences (100000085 bp) in 1.640 CPU sec, 1.635 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282551, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 334, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (346.97, 153.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (374, 665, 2852)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7808)
[M::mem_pestat] mean and std.dev: (1226.60, 1072.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10286)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 678208 reads in 168.522 CPU sec, 5.363 real sec
sorting block 130, #reads = 1075178 mem = 536882951 peak = 1264914432
[M::mem_process_seqs] Processed 677174 reads in 104.985 CPU sec, 3.332 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 282481, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 331, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (344.61, 151.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 131, #reads = 1073669 mem = 536881357 peak = 1267159040
[M::process] read 676832 sequences (100000245 bp) in 2.740 CPU sec, 2.948 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282053, 1, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 336, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (348.75, 152.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (77, 186, 186)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404)
[M::mem_pestat] mean and std.dev: (131.50, 54.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 513)
[M::mem_pestat] skip orientation RR
[M::process] read 677986 sequences (100000065 bp) in 1.680 CPU sec, 1.681 real sec...
[M::mem_process_seqs] Processed 677498 reads in 164.239 CPU sec, 5.220 real sec
[M::mem_process_seqs] Processed 676894 reads in 106.177 CPU sec, 3.371 real sec
sorting block 132, #reads = 1073416 mem = 536883339 peak = 1267159040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282236, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 337, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (349.98, 153.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677190 sequences (100000259 bp) in 2.392 CPU sec, 2.471 real sec...
[M::process] read 676198 sequences (100000010 bp) in 1.610 CPU sec, 1.613 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282734, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 329, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (344.55, 154.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676832 reads in 166.857 CPU sec, 5.310 real sec
[M::mem_process_seqs] Processed 677986 reads in 108.341 CPU sec, 3.451 real sec
sorting block 133, #reads = 1074276 mem = 536879232 peak = 1267159040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282426, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 335, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (348.44, 153.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676042 sequences (100000182 bp) in 2.722 CPU sec, 2.925 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281989, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 342, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (355.11, 155.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676888 sequences (100000144 bp) in 1.630 CPU sec, 1.629 real sec...
[M::mem_process_seqs] Processed 677190 reads in 162.393 CPU sec, 5.160 real sec
[M::mem_process_seqs] Processed 676198 reads in 102.850 CPU sec, 3.269 real sec
sorting block 134, #reads = 1073482 mem = 536877537 peak = 1267159040
[M::process] read 676258 sequences (100000281 bp) in 2.428 CPU sec, 2.501 real sec...
[M::process] read 675382 sequences (100000272 bp) in 1.590 CPU sec, 1.593 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281741, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 343, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (359.26, 160.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676258 reads in 84.868 CPU sec, 2.775 real sec
sorting block 135, #reads = 1074977 mem = 536878065 peak = 1267159040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282345, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 335, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (351.65, 157.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676888 reads in 172.412 CPU sec, 6.005 real sec
sorting block 136, #reads = 1075902 mem = 536879384 peak = 1267367936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281765, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 345, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (359.38, 158.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675432 sequences (100000212 bp) in 2.412 CPU sec, 2.513 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281584, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 351, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (365.44, 161.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676042 reads in 333.376 CPU sec, 11.129 real sec
[M::mem_process_seqs] Processed 675382 reads in 119.912 CPU sec, 3.803 real sec
[M::process] read 675382 sequences (100000093 bp) in 2.007 CPU sec, 2.095 real sec...
sorting block 137, #reads = 1075250 mem = 536878613 peak = 1267367936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281655, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 353, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (368.32, 163.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675264 sequences (100000157 bp) in 2.511 CPU sec, 2.750 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282013, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 354, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (369.14, 165.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675432 reads in 160.770 CPU sec, 5.121 real sec
[M::process] read 675258 sequences (100000183 bp) in 1.619 CPU sec, 1.633 real sec...
[M::mem_process_seqs] Processed 675382 reads in 104.239 CPU sec, 3.302 real sec
sorting block 138, #reads = 1075427 mem = 536879523 peak = 1267367936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282011, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 355, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (370.81, 166.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674946 sequences (100000215 bp) in 2.825 CPU sec, 3.060 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281589, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 355, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (370.20, 165.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675264 reads in 160.830 CPU sec, 5.121 real sec
[M::process] read 674426 sequences (100000230 bp) in 1.610 CPU sec, 1.604 real sec...
sorting block 139, #reads = 1075195 mem = 536878981 peak = 1267367936
[M::mem_process_seqs] Processed 675258 reads in 104.889 CPU sec, 3.326 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281870, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 354, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (369.12, 163.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674882 sequences (100000103 bp) in 2.393 CPU sec, 2.631 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281751, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 358, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (372.98, 164.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674772 sequences (100000009 bp) in 1.620 CPU sec, 1.618 real sec...
[M::mem_process_seqs] Processed 674946 reads in 159.792 CPU sec, 5.091 real sec
sorting block 140, #reads = 1074370 mem = 536878557 peak = 1267367936
[M::mem_process_seqs] Processed 674426 reads in 103.234 CPU sec, 3.280 real sec
sorting block 141, #reads = 1074487 mem = 536885240 peak = 1267367936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281952, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 358, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (373.66, 166.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675200 sequences (100000099 bp) in 2.631 CPU sec, 2.846 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281390, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 359, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (374.52, 166.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678388 sequences (100000256 bp) in 1.700 CPU sec, 1.701 real sec...
[M::mem_process_seqs] Processed 674882 reads in 165.709 CPU sec, 5.270 real sec
[M::mem_process_seqs] Processed 674772 reads in 107.027 CPU sec, 3.392 real sec
sorting block 142, #reads = 1073384 mem = 536878709 peak = 1267367936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281634, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 354, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (369.11, 164.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679766 sequences (100000232 bp) in 2.687 CPU sec, 2.924 real sec...
[M::process] read 679134 sequences (100000011 bp) in 1.630 CPU sec, 1.630 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283103, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 327, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (344.87, 157.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675200 reads in 178.132 CPU sec, 5.670 real sec
[M::mem_process_seqs] Processed 678388 reads in 117.428 CPU sec, 3.744 real sec
sorting block 143, #reads = 1070495 mem = 536876781 peak = 1267367936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282796, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 317, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (337.12, 156.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678366 sequences (100000183 bp) in 2.339 CPU sec, 2.421 real sec...
[M::process] read 678152 sequences (100000242 bp) in 1.640 CPU sec, 1.640 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283176, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 322, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (340.79, 157.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679766 reads in 161.068 CPU sec, 5.122 real sec
sorting block 144, #reads = 1068623 mem = 536878697 peak = 1267367936
[M::mem_process_seqs] Processed 679134 reads in 100.971 CPU sec, 3.217 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282791, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 327, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (343.40, 155.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 145, #reads = 1077298 mem = 536878281 peak = 1270452224
[M::process] read 677824 sequences (100000142 bp) in 2.361 CPU sec, 2.459 real sec...
[M::process] read 677444 sequences (100000141 bp) in 1.600 CPU sec, 1.594 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 282491, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 328, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (343.46, 154.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678366 reads in 156.715 CPU sec, 4.988 real sec
[M::mem_process_seqs] Processed 678152 reads in 98.685 CPU sec, 3.139 real sec
sorting block 146, #reads = 1078471 mem = 536883387 peak = 1270452224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 282709, 2, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 379, 747)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1943)
[M::mem_pestat] mean and std.dev: (487.56, 420.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2541)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 329, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (345.04, 155.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 677120 sequences (100000237 bp) in 2.328 CPU sec, 2.400 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282316, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 332, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (348.00, 156.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677118 sequences (100000277 bp) in 1.600 CPU sec, 1.609 real sec...
[M::mem_process_seqs] Processed 677824 reads in 152.270 CPU sec, 4.849 real sec
[M::mem_process_seqs] Processed 677444 reads in 97.423 CPU sec, 3.097 real sec
sorting block 147, #reads = 1078293 mem = 536878050 peak = 1270452224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282553, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 334, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (349.63, 156.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677096 sequences (100000179 bp) in 2.734 CPU sec, 2.960 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282076, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 338, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (353.55, 159.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677120 reads in 152.227 CPU sec, 4.845 real sec
[M::process] read 676758 sequences (100000048 bp) in 1.644 CPU sec, 1.649 real sec...
[M::mem_process_seqs] Processed 677118 reads in 98.590 CPU sec, 3.128 real sec
sorting block 148, #reads = 1078519 mem = 536880988 peak = 1270452224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282845, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 338, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (354.84, 160.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676604 sequences (100000191 bp) in 2.622 CPU sec, 2.781 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282080, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 341, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (356.00, 160.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676516 sequences (100000156 bp) in 1.610 CPU sec, 1.613 real sec...
[M::mem_process_seqs] Processed 677096 reads in 151.826 CPU sec, 4.829 real sec
sorting block 149, #reads = 1078287 mem = 536880856 peak = 1270452224
[M::mem_process_seqs] Processed 676758 reads in 98.005 CPU sec, 3.106 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281827, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 342, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (357.98, 161.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 150, #reads = 1078175 mem = 536879811 peak = 1270452224
[M::process] read 676656 sequences (100000115 bp) in 2.798 CPU sec, 3.033 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282103, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 341, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (356.49, 159.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676604 reads in 151.423 CPU sec, 4.821 real sec
[M::mem_process_seqs] Processed 676516 reads in 97.651 CPU sec, 3.091 real sec
[M::process] read 676306 sequences (100000247 bp) in 2.017 CPU sec, 2.057 real sec...
sorting block 151, #reads = 1079060 mem = 536878502 peak = 1270452224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282041, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 341, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (355.87, 159.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676686 sequences (100000062 bp) in 2.393 CPU sec, 2.482 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282566, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 342, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (356.69, 158.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676656 reads in 154.725 CPU sec, 4.928 real sec
[M::process] read 676494 sequences (100000262 bp) in 1.779 CPU sec, 1.778 real sec...
[M::mem_process_seqs] Processed 676306 reads in 101.412 CPU sec, 3.210 real sec
sorting block 152, #reads = 1077871 mem = 536882129 peak = 1270452224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 282464, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 339, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (353.45, 157.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675954 sequences (100000017 bp) in 2.582 CPU sec, 2.677 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281902, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 343, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (357.36, 159.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675690 sequences (100000062 bp) in 1.680 CPU sec, 1.684 real sec...
[M::mem_process_seqs] Processed 676686 reads in 157.036 CPU sec, 4.991 real sec
[M::mem_process_seqs] Processed 676494 reads in 99.219 CPU sec, 3.147 real sec
sorting block 153, #reads = 1076430 mem = 536880967 peak = 1270452224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281432, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 345, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (360.77, 160.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675502 sequences (100000090 bp) in 2.405 CPU sec, 2.541 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281464, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 345, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (359.01, 157.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675710 sequences (100000250 bp) in 1.630 CPU sec, 1.630 real sec...
[M::mem_process_seqs] Processed 675954 reads in 157.534 CPU sec, 5.013 real sec
sorting block 154, #reads = 1076253 mem = 536878599 peak = 1270452224
[M::mem_process_seqs] Processed 675690 reads in 98.766 CPU sec, 3.134 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281893, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 348, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (362.15, 159.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 155, #reads = 1076587 mem = 536878989 peak = 1270452224
[M::process] read 675492 sequences (100000262 bp) in 2.490 CPU sec, 2.718 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281654, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 349, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (364.09, 162.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675132 sequences (100000111 bp) in 1.580 CPU sec, 1.581 real sec...
[M::mem_process_seqs] Processed 675502 reads in 153.979 CPU sec, 4.900 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281332, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 350, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (364.07, 161.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675068 sequences (100000215 bp) in 1.809 CPU sec, 1.817 real sec...
[M::mem_process_seqs] Processed 675492 reads in 90.500 CPU sec, 3.082 real sec
sorting block 156, #reads = 1077004 mem = 536877990 peak = 1270452224
[M::mem_process_seqs] Processed 675710 reads in 216.387 CPU sec, 7.090 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281152, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 354, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (369.75, 165.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674986 sequences (100000234 bp) in 2.597 CPU sec, 2.845 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 281948, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 353, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (368.14, 163.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675132 reads in 171.738 CPU sec, 5.472 real sec
sorting block 157, #reads = 1076949 mem = 536877555 peak = 1270452224
[M::mem_process_seqs] Processed 675068 reads in 101.738 CPU sec, 3.221 real sec
[M::process] read 675278 sequences (100000270 bp) in 1.973 CPU sec, 2.040 real sec...
sorting block 158, #reads = 1076824 mem = 536886947 peak = 1270452224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281602, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 352, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (366.67, 161.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675086 sequences (100000020 bp) in 2.004 CPU sec, 2.116 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282127, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 350, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (365.22, 162.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674884 sequences (100000054 bp) in 1.590 CPU sec, 1.589 real sec...
[M::mem_process_seqs] Processed 674986 reads in 158.068 CPU sec, 5.045 real sec
[M::mem_process_seqs] Processed 675278 reads in 102.085 CPU sec, 3.243 real sec
sorting block 159, #reads = 1076324 mem = 536881404 peak = 1270452224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281151, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 353, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (368.60, 163.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675366 sequences (100000176 bp) in 2.419 CPU sec, 2.523 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281338, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 350, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (364.84, 160.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675124 sequences (100000216 bp) in 1.620 CPU sec, 1.624 real sec...
[M::mem_process_seqs] Processed 675086 reads in 159.429 CPU sec, 5.075 real sec
[M::mem_process_seqs] Processed 674884 reads in 101.493 CPU sec, 3.220 real sec
sorting block 160, #reads = 1075947 mem = 536878499 peak = 1270452224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281951, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 350, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (365.00, 162.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675052 sequences (100000054 bp) in 2.431 CPU sec, 2.684 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281870, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 351, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (366.56, 162.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675366 reads in 157.601 CPU sec, 5.019 real sec
[M::process] read 675256 sequences (100000058 bp) in 1.640 CPU sec, 1.640 real sec...
sorting block 161, #reads = 1075409 mem = 536877828 peak = 1270452224
[M::mem_process_seqs] Processed 675124 reads in 101.540 CPU sec, 3.219 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281491, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 351, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (366.89, 163.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 162, #reads = 1075777 mem = 536878434 peak = 1270452224
[M::process] read 675144 sequences (100000032 bp) in 2.735 CPU sec, 2.957 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282135, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 349, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (365.52, 162.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675052 reads in 155.040 CPU sec, 4.926 real sec
[M::process] read 675496 sequences (100000057 bp) in 1.705 CPU sec, 1.710 real sec...
[M::mem_process_seqs] Processed 675256 reads in 100.941 CPU sec, 3.196 real sec
sorting block 163, #reads = 1075889 mem = 536879570 peak = 1270452224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281209, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 353, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (368.60, 164.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675070 sequences (100000037 bp) in 2.255 CPU sec, 2.385 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281248, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 349, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (365.71, 164.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674922 sequences (100000134 bp) in 1.670 CPU sec, 1.675 real sec...
[M::mem_process_seqs] Processed 675144 reads in 154.671 CPU sec, 4.919 real sec
[M::mem_process_seqs] Processed 675496 reads in 99.688 CPU sec, 3.163 real sec
sorting block 164, #reads = 1074613 mem = 536879055 peak = 1270452224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281747, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 349, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (365.11, 162.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674900 sequences (100000278 bp) in 2.360 CPU sec, 2.444 real sec...
[M::process] read 680814 sequences (100000100 bp) in 1.700 CPU sec, 1.702 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281324, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 351, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (368.10, 164.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675070 reads in 158.733 CPU sec, 5.040 real sec
[M::mem_process_seqs] Processed 674922 reads in 102.613 CPU sec, 3.256 real sec
sorting block 165, #reads = 1074813 mem = 536879182 peak = 1270452224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281222, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 355, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (372.55, 168.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682914 sequences (100000009 bp) in 2.161 CPU sec, 2.230 real sec...
[M::process] read 681422 sequences (100000174 bp) in 1.700 CPU sec, 1.698 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283309, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 311, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (330.39, 154.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1101)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674900 reads in 177.961 CPU sec, 5.672 real sec
sorting block 166, #reads = 1072413 mem = 536877892 peak = 1270452224
[M::mem_process_seqs] Processed 680814 reads in 117.336 CPU sec, 3.729 real sec
sorting block 167, #reads = 1065661 mem = 536877645 peak = 1270452224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284518, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 297, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (315.93, 145.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680458 sequences (100000078 bp) in 2.203 CPU sec, 2.276 real sec...
[M::process] read 680014 sequences (100000088 bp) in 1.750 CPU sec, 1.753 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283600, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 305, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (322.16, 146.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682914 reads in 174.862 CPU sec, 5.569 real sec
[M::mem_process_seqs] Processed 681422 reads in 111.196 CPU sec, 3.532 real sec
sorting block 168, #reads = 1073211 mem = 536879058 peak = 1270452224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283556, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 312, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862)
[M::mem_pestat] mean and std.dev: (327.89, 148.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678848 sequences (100000100 bp) in 2.201 CPU sec, 2.288 real sec...
[M::process] read 679006 sequences (100000038 bp) in 1.730 CPU sec, 1.723 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283848, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 315, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (328.98, 146.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680458 reads in 168.569 CPU sec, 5.373 real sec
[M::mem_process_seqs] Processed 680014 reads in 108.099 CPU sec, 3.433 real sec
sorting block 169, #reads = 1072999 mem = 536880938 peak = 1270452224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282754, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 320, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (332.66, 146.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1067)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678598 sequences (100000187 bp) in 2.721 CPU sec, 2.891 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283679, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 322, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868)
[M::mem_pestat] mean and std.dev: (335.31, 148.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1084)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678536 sequences (100000220 bp) in 1.660 CPU sec, 1.658 real sec...
[M::mem_process_seqs] Processed 678848 reads in 165.466 CPU sec, 5.260 real sec
[M::mem_process_seqs] Processed 679006 reads in 107.258 CPU sec, 3.402 real sec
sorting block 170, #reads = 1072856 mem = 536878688 peak = 1270452224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282371, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 325, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (339.38, 151.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1105)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677918 sequences (100000080 bp) in 2.690 CPU sec, 2.886 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282596, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 327, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (341.72, 153.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677512 sequences (100000065 bp) in 1.660 CPU sec, 1.663 real sec...
[M::mem_process_seqs] Processed 678598 reads in 178.008 CPU sec, 5.669 real sec
sorting block 171, #reads = 1073558 mem = 536877219 peak = 1270452224
[M::mem_process_seqs] Processed 678536 reads in 110.573 CPU sec, 3.512 real sec
sorting block 172, #reads = 1070474 mem = 536882336 peak = 1270452224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282787, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 330, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (345.04, 153.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677202 sequences (100000277 bp) in 2.775 CPU sec, 2.980 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282026, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 333, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (346.92, 154.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677000 sequences (100000159 bp) in 1.680 CPU sec, 1.675 real sec...
[M::mem_process_seqs] Processed 677918 reads in 165.078 CPU sec, 5.239 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282381, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 337, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (351.13, 155.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677482 sequences (100000122 bp) in 1.778 CPU sec, 1.778 real sec...
[M::mem_process_seqs] Processed 677202 reads in 91.944 CPU sec, 3.064 real sec
sorting block 173, #reads = 1073472 mem = 536881626 peak = 1270452224
[M::mem_process_seqs] Processed 677512 reads in 218.276 CPU sec, 7.082 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281977, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 336, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (350.25, 155.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 174, #reads = 1074019 mem = 536879451 peak = 1270452224
[M::process] read 677294 sequences (100000116 bp) in 2.356 CPU sec, 2.486 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282734, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 331, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (344.81, 151.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676152 sequences (100000036 bp) in 1.620 CPU sec, 1.620 real sec...
[M::mem_process_seqs] Processed 677000 reads in 180.349 CPU sec, 5.724 real sec
[M::mem_process_seqs] Processed 677482 reads in 107.285 CPU sec, 3.402 real sec
sorting block 175, #reads = 1073864 mem = 536882244 peak = 1270452224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282558, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 332, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (345.29, 151.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675600 sequences (100000021 bp) in 2.399 CPU sec, 2.526 real sec...
[M::process] read 675404 sequences (100000234 bp) in 1.610 CPU sec, 1.612 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281967, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 341, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (353.98, 154.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677294 reads in 162.242 CPU sec, 5.151 real sec
[M::mem_process_seqs] Processed 676152 reads in 104.555 CPU sec, 3.332 real sec
sorting block 176, #reads = 1073374 mem = 536885645 peak = 1270452224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281729, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 347, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (359.44, 155.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676428 sequences (100000096 bp) in 2.346 CPU sec, 2.427 real sec...
[M::process] read 676216 sequences (100000080 bp) in 1.660 CPU sec, 1.660 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281474, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 346, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (358.04, 154.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675600 reads in 159.660 CPU sec, 5.074 real sec
[M::mem_process_seqs] Processed 675404 reads in 102.588 CPU sec, 3.257 real sec
sorting block 177, #reads = 1072433 mem = 536880086 peak = 1270452224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282434, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 338, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (351.43, 153.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675978 sequences (100000136 bp) in 2.454 CPU sec, 2.585 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282458, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 339, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (352.58, 153.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675878 sequences (100000187 bp) in 1.630 CPU sec, 1.630 real sec...
[M::mem_process_seqs] Processed 676428 reads in 161.247 CPU sec, 5.129 real sec
sorting block 178, #reads = 1074119 mem = 536879471 peak = 1270452224
[M::process] read 675644 sequences (100000124 bp) in 1.819 CPU sec, 1.822 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281923, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 340, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (353.30, 154.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675978 reads in 92.961 CPU sec, 3.175 real sec
sorting block 179, #reads = 1073457 mem = 536878663 peak = 1270452224
[M::mem_process_seqs] Processed 676216 reads in 227.197 CPU sec, 7.459 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281471, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 341, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (354.42, 154.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675546 sequences (100000034 bp) in 2.571 CPU sec, 2.801 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281230, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 343, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (356.80, 155.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675878 reads in 173.820 CPU sec, 5.535 real sec
sorting block 180, #reads = 1074914 mem = 536877825 peak = 1270452224
[M::mem_process_seqs] Processed 675644 reads in 103.288 CPU sec, 3.261 real sec
[M::process] read 675788 sequences (100000097 bp) in 1.883 CPU sec, 1.932 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281412, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 343, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (356.72, 155.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 181, #reads = 1075366 mem = 536878022 peak = 1270452224
[M::process] read 675784 sequences (100000058 bp) in 1.996 CPU sec, 2.115 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282055, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 343, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (357.19, 157.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675480 sequences (100000014 bp) in 1.580 CPU sec, 1.572 real sec...
[M::mem_process_seqs] Processed 675546 reads in 155.194 CPU sec, 4.940 real sec
[M::mem_process_seqs] Processed 675788 reads in 98.698 CPU sec, 3.130 real sec
sorting block 182, #reads = 1075191 mem = 536881444 peak = 1270452224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280970, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 344, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (358.97, 158.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675378 sequences (100000025 bp) in 2.230 CPU sec, 2.330 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281424, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 347, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (361.87, 159.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675304 sequences (100000268 bp) in 1.610 CPU sec, 1.614 real sec...
[M::mem_process_seqs] Processed 675784 reads in 155.358 CPU sec, 4.951 real sec
[M::mem_process_seqs] Processed 675480 reads in 99.218 CPU sec, 3.152 real sec
sorting block 183, #reads = 1075457 mem = 536877493 peak = 1270452224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281479, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 348, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (363.06, 160.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675210 sequences (100000006 bp) in 2.130 CPU sec, 2.202 real sec...
[M::process] read 675018 sequences (100000096 bp) in 1.630 CPU sec, 1.628 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280768, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 348, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (362.56, 159.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675378 reads in 155.904 CPU sec, 4.952 real sec
[M::mem_process_seqs] Processed 675304 reads in 100.361 CPU sec, 3.185 real sec
sorting block 184, #reads = 1075912 mem = 536880422 peak = 1270452224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281764, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 348, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (364.02, 161.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673880 sequences (100000109 bp) in 2.335 CPU sec, 2.420 real sec...
[M::process] read 674730 sequences (100000141 bp) in 1.620 CPU sec, 1.624 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281790, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 352, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (368.60, 163.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675210 reads in 159.563 CPU sec, 5.066 real sec
sorting block 185, #reads = 1074333 mem = 536878991 peak = 1270452224
[M::mem_process_seqs] Processed 675018 reads in 101.061 CPU sec, 3.213 real sec
sorting block 186, #reads = 1074790 mem = 536880874 peak = 1270452224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281071, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 364, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (378.69, 166.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674630 sequences (100000195 bp) in 2.188 CPU sec, 2.254 real sec...
[M::process] read 237782 sequences (35205492 bp) in 0.780 CPU sec, 0.785 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281193, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 353, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (368.48, 164.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673880 reads in 152.478 CPU sec, 4.869 real sec
[M::mem_process_seqs] Processed 674730 reads in 97.416 CPU sec, 3.112 real sec
sorting block 187, #reads = 1075175 mem = 536877724 peak = 1270452224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281051, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 353, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (369.06, 163.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 99121, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 345, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (360.47, 158.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674630 reads in 111.848 CPU sec, 3.633 real sec
[M::mem_process_seqs] Processed 237782 reads in 47.041 CPU sec, 1.528 real sec
sorting block 188, #reads = 1073861 mem = 536877828 peak = 1270452224
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000606a6
  features: 1f8bfbff fffa320b
  extended: f1bf07ab 00407f5a
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8375C CPU @ 2.90GHz
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 203012022
overall: 69915742208 mem 28121.592 user 857.040 sys 985.157 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_bR4-T1-TDNA-1_A23MHWWLT4_1.L007\tPU:L007\tSM:659_bR4-T1-TDNA-1_A23MHWWLT4_1\tLB:659_bR4-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_bR4-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_bR4-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 985.189 sec; CPU: 28978.635 sec
sorting block 189, #reads = 222792 mem = 111859553 peak = 1270452224
uniq header count 1
merging 190 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000606a6
  features: 1f8bfbff fffa320b
  extended: f1bf07ab 00407f5a
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8375C CPU @ 2.90GHz
threads: 31 max 128
algo: util-sort
license: sentieon:util=1
output file size: 11204359467
output reads: 203851562
bam_mem_sort: 190 calls 148.831 user 3.344 sys 152.930 real
bam_write: 190 calls 140.318 user 6.033 sys 159.958 real
execute: 1 calls 48.428 user 2.239 sys 1061.404 real
merge_files: 1 calls 48.400 user 2.193 sys 75.779 real
parse_chunk: 3028 calls 156.018 user 33.804 sys 196.464 real
read_chunk: 8156 calls 74.224 user 50.853 sys 897.804 real
sort_block: 1 calls 50.129 user 49.263 sys 861.226 real
write_chunk: 5126 calls 47.804 user 1.832 sys 74.406 real
overall: 3480973312 mem 3035.465 user 131.768 sys 1061.426 real
ls: cannot access '*.cram': No such file or directory