This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_5Q-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1843.020 CPU sec, 68.363 real sec [M::process] read 685114 sequences (100000106 bp) in 1.133 CPU sec, 1.134 real sec... [M::process] read 684180 sequences (100000014 bp) in 2.091 CPU sec, 2.137 real sec... [M::process] read 683670 sequences (100000010 bp) in 2.048 CPU sec, 2.077 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282457, 8, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 168, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (182.04, 73.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 298, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (317.63, 152.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1084) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (143, 175, 555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1379) [M::mem_pestat] mean and std.dev: (319.28, 240.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1791) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683440 sequences (100000064 bp) in 2.007 CPU sec, 2.037 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282651, 3, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 242, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (305.70, 178.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1078) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 305, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (322.73, 153.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1100) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 246, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (220.63, 72.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282281, 4, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 236, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (270.70, 129.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 306, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (323.55, 153.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (129, 225, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (245.80, 134.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685114 reads in 347.628 CPU sec, 11.527 real sec [M::mem_process_seqs] Processed 684180 reads in 235.466 CPU sec, 7.916 real sec [M::mem_process_seqs] Processed 683670 reads in 262.147 CPU sec, 8.822 real sec sorting block 0, #reads = 1080173 mem = 536881315 peak = 595845120 [M::process] read 682876 sequences (100000147 bp) in 2.581 CPU sec, 2.754 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 282386, 0, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 204, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (315.50, 213.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1177) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 309, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (326.15, 154.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 409, 630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1458) [M::mem_pestat] mean and std.dev: (326.18, 137.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1872) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682470 sequences (100000182 bp) in 2.267 CPU sec, 2.430 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282553, 7, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 285, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846) [M::mem_pestat] mean and std.dev: (270.56, 105.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1067) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 312, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (329.48, 155.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (137, 237, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013) [M::mem_pestat] mean and std.dev: (277.55, 161.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1305) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682156 sequences (100000300 bp) in 1.960 CPU sec, 1.986 real sec... [M::mem_process_seqs] Processed 683440 reads in 211.674 CPU sec, 6.950 real sec sorting block 1, #reads = 1079656 mem = 536877204 peak = 967577600 [M::mem_process_seqs] Processed 682876 reads in 144.647 CPU sec, 4.722 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 282490, 5, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 288, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062) [M::mem_pestat] mean and std.dev: (293.94, 181.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1366) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 313, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (329.33, 153.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 233, 592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1400) [M::mem_pestat] mean and std.dev: (325.54, 206.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1804) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681420 sequences (100000175 bp) in 2.275 CPU sec, 2.502 real sec... sorting block 2, #reads = 1079412 mem = 536878133 peak = 969678848 [M::process] read 681304 sequences (100000292 bp) in 2.156 CPU sec, 2.205 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282104, 2, 25) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 316, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (332.78, 156.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (257, 344, 753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1745) [M::mem_pestat] mean and std.dev: (456.00, 395.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2241) [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682470 reads in 210.943 CPU sec, 6.867 real sec [M::mem_process_seqs] Processed 682156 reads in 147.304 CPU sec, 4.791 real sec sorting block 3, #reads = 1079302 mem = 536879164 peak = 969678848 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 282229, 5, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 187, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044) [M::mem_pestat] mean and std.dev: (252.38, 171.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1346) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 320, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (336.08, 157.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 271, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977) [M::mem_pestat] mean and std.dev: (268.32, 155.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1250) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681038 sequences (100000068 bp) in 2.678 CPU sec, 2.953 real sec... [M::process] read 681290 sequences (100000267 bp) in 2.030 CPU sec, 2.034 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 281791, 10, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 376, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1176) [M::mem_pestat] mean and std.dev: (322.12, 180.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 323, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (338.14, 157.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1119, 1401, 1401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (555, 1965) [M::mem_pestat] mean and std.dev: (1373.75, 184.67) [M::mem_pestat] low and high boundaries for proper pairs: (273, 2247) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 229, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (234.70, 105.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681420 reads in 215.350 CPU sec, 7.097 real sec [M::mem_process_seqs] Processed 681304 reads in 149.916 CPU sec, 4.922 real sec sorting block 4, #reads = 1077940 mem = 536877114 peak = 969678848 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 281592, 1, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 245, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005) [M::mem_pestat] mean and std.dev: (284.61, 196.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1282) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 320, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (336.07, 156.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 321, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (305.46, 125.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681016 sequences (100000232 bp) in 2.580 CPU sec, 2.935 real sec... [M::process] read 681162 sequences (100000119 bp) in 2.110 CPU sec, 2.131 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281849, 3, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 239, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (232.16, 83.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 321, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (336.36, 156.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 287, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844) [M::mem_pestat] mean and std.dev: (300.04, 137.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1064) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681038 reads in 218.101 CPU sec, 7.260 real sec sorting block 5, #reads = 1077859 mem = 536880555 peak = 969678848 [M::mem_process_seqs] Processed 681290 reads in 143.406 CPU sec, 4.668 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 281727, 3, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 320, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989) [M::mem_pestat] mean and std.dev: (279.75, 129.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1263) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 319, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (335.91, 155.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (142, 211, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (205.18, 80.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681434 sequences (100000165 bp) in 2.780 CPU sec, 3.066 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 281240, 11, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 200, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1123) [M::mem_pestat] mean and std.dev: (256.08, 232.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 321, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (337.72, 157.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1043, 1990, 4027) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9995) [M::mem_pestat] mean and std.dev: (2295.18, 1292.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12979) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (120, 149, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (177.22, 95.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680942 sequences (100000248 bp) in 2.382 CPU sec, 2.385 real sec... [M::mem_process_seqs] Processed 681016 reads in 205.643 CPU sec, 6.646 real sec sorting block 6, #reads = 1079330 mem = 536879490 peak = 983719936 [M::mem_process_seqs] Processed 681162 reads in 144.632 CPU sec, 4.611 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 281513, 10, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 265, 1039) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2743) [M::mem_pestat] mean and std.dev: (389.06, 352.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 316, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (333.73, 156.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (451, 770, 964) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1990) [M::mem_pestat] mean and std.dev: (766.00, 343.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2503) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 242, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 508) [M::mem_pestat] mean and std.dev: (248.15, 84.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 7, #reads = 1078937 mem = 536880240 peak = 993968128 [M::process] read 680492 sequences (100000018 bp) in 2.569 CPU sec, 3.057 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 281019, 8, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 263, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (265.31, 85.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 322, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (337.14, 156.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 248, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (247.65, 79.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680448 sequences (100000281 bp) in 2.506 CPU sec, 2.531 real sec... [M::mem_process_seqs] Processed 681434 reads in 204.609 CPU sec, 6.631 real sec [M::mem_process_seqs] Processed 680942 reads in 143.824 CPU sec, 4.601 real sec sorting block 8, #reads = 1078369 mem = 536877236 peak = 993968128 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281580, 4, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 211, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (236.22, 109.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 324, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (339.12, 156.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 237, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (257.31, 128.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680668 sequences (100000180 bp) in 3.162 CPU sec, 3.706 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281331, 5, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 228, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1158) [M::mem_pestat] mean and std.dev: (322.90, 197.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 320, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (335.44, 154.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (135, 167, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (141.94, 40.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 451) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680492 reads in 204.044 CPU sec, 6.615 real sec [M::process] read 680750 sequences (100000051 bp) in 2.715 CPU sec, 2.786 real sec... [M::mem_process_seqs] Processed 680448 reads in 143.685 CPU sec, 4.632 real sec sorting block 9, #reads = 1077557 mem = 536878777 peak = 993968128 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 280972, 12, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 210, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (233.31, 135.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 319, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (334.17, 153.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (591, 939, 2659) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6795) [M::mem_pestat] mean and std.dev: (1185.42, 889.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8863) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 274, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (315.83, 124.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680360 sequences (100000125 bp) in 2.694 CPU sec, 3.299 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 281026, 8, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 205, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (214.30, 65.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 317, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (333.21, 153.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1104) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 223, 840) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2118) [M::mem_pestat] mean and std.dev: (370.62, 283.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2757) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680872 sequences (100000056 bp) in 2.324 CPU sec, 2.335 real sec... [M::mem_process_seqs] Processed 680668 reads in 207.192 CPU sec, 6.753 real sec sorting block 10, #reads = 1078709 mem = 536878452 peak = 1009590272 [M::mem_process_seqs] Processed 680750 reads in 143.884 CPU sec, 4.649 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 280686, 4, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 279, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (250.91, 97.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 318, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (334.01, 153.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 472, 574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1240) [M::mem_pestat] mean and std.dev: (438.00, 243.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1573) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 11, #reads = 1076912 mem = 536879706 peak = 1009590272 [M::process] read 681092 sequences (100000003 bp) in 3.100 CPU sec, 3.587 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 281180, 5, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 166, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (206.21, 86.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 318, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (333.80, 153.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 309, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018) [M::mem_pestat] mean and std.dev: (295.48, 149.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1303) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680788 sequences (100000145 bp) in 2.317 CPU sec, 2.345 real sec... [M::mem_process_seqs] Processed 680360 reads in 210.295 CPU sec, 6.804 real sec [M::mem_process_seqs] Processed 680872 reads in 148.234 CPU sec, 4.802 real sec sorting block 12, #reads = 1075619 mem = 536876271 peak = 1009590272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 281372, 8, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 306, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (291.78, 141.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 316, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (331.59, 152.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 192, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (210.27, 69.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680886 sequences (100000195 bp) in 2.918 CPU sec, 3.437 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 281134, 8, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 298, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (264.07, 97.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 317, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (333.26, 154.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 322, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (277.33, 114.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681092 reads in 207.841 CPU sec, 6.716 real sec [M::process] read 680802 sequences (100000150 bp) in 2.404 CPU sec, 2.420 real sec... [M::mem_process_seqs] Processed 680788 reads in 144.819 CPU sec, 4.649 real sec sorting block 13, #reads = 1076660 mem = 536876853 peak = 1009590272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 281980, 3, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 215, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969) [M::mem_pestat] mean and std.dev: (302.52, 247.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1291) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 318, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (333.97, 154.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 262, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (261.91, 86.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680684 sequences (100000228 bp) in 3.064 CPU sec, 3.722 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 281279, 4, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 188, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1286) [M::mem_pestat] mean and std.dev: (248.50, 171.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 318, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (333.90, 154.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (336, 517, 565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023) [M::mem_pestat] mean and std.dev: (457.46, 186.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1252) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680886 reads in 208.079 CPU sec, 6.752 real sec [M::mem_process_seqs] Processed 680802 reads in 145.061 CPU sec, 4.647 real sec [M::process] read 680536 sequences (100000085 bp) in 2.733 CPU sec, 2.806 real sec... sorting block 14, #reads = 1077351 mem = 536878346 peak = 1009590272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 281178, 4, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 217, 546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1256) [M::mem_pestat] mean and std.dev: (319.97, 253.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 319, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (335.57, 155.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 264, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (267.82, 75.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680216 sequences (100000158 bp) in 3.541 CPU sec, 3.909 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 281202, 4, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 289, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (47, 512) [M::mem_pestat] mean and std.dev: (258.04, 71.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 323, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (339.32, 158.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1138) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (246, 324, 784) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1860) [M::mem_pestat] mean and std.dev: (379.05, 214.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2398) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680684 reads in 207.817 CPU sec, 6.720 real sec sorting block 15, #reads = 1077115 mem = 536879856 peak = 1009590272 [M::mem_process_seqs] Processed 680536 reads in 147.188 CPU sec, 4.717 real sec [M::process] read 681656 sequences (100000078 bp) in 2.836 CPU sec, 2.951 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 281463, 3, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 273, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (257.67, 115.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 324, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (340.82, 158.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 288, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (294.96, 104.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 16, #reads = 1076056 mem = 536877633 peak = 1009590272 [M::process] read 684420 sequences (100000293 bp) in 3.656 CPU sec, 4.305 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282273, 13, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 315, 549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1303) [M::mem_pestat] mean and std.dev: (289.84, 166.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 317, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (334.38, 157.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (97, 1022, 1793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5185) [M::mem_pestat] mean and std.dev: (1119.08, 780.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6881) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (244, 308, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952) [M::mem_pestat] mean and std.dev: (289.00, 110.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1188) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680216 reads in 204.299 CPU sec, 6.645 real sec [M::mem_process_seqs] Processed 681656 reads in 147.042 CPU sec, 4.750 real sec [M::process] read 683636 sequences (100000087 bp) in 2.698 CPU sec, 2.916 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 282607, 5, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 194, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (192.83, 63.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 302, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (320.10, 152.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1083) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 247, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (243.32, 119.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 17, #reads = 1076756 mem = 536877734 peak = 1009590272 [M::process] read 683144 sequences (100000210 bp) in 2.807 CPU sec, 3.246 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 281952, 3, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 204, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039) [M::mem_pestat] mean and std.dev: (279.12, 279.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1398) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 306, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (323.74, 153.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1097) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 279, 1026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2770) [M::mem_pestat] mean and std.dev: (263.20, 198.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3642) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684420 reads in 198.133 CPU sec, 6.483 real sec [M::mem_process_seqs] Processed 683636 reads in 138.890 CPU sec, 4.486 real sec [M::process] read 682438 sequences (100000122 bp) in 2.722 CPU sec, 2.867 real sec... sorting block 18, #reads = 1079188 mem = 536879756 peak = 1009590272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 281999, 5, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (283, 404, 1405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3649) [M::mem_pestat] mean and std.dev: (578.42, 486.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4771) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 309, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (324.94, 152.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1088) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (252, 375, 2109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5823) [M::mem_pestat] mean and std.dev: (780.07, 849.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7680) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682450 sequences (100000065 bp) in 2.994 CPU sec, 3.577 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282122, 7, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 242, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (289.12, 135.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 310, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (325.54, 151.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1082) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 263, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (274.60, 105.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683144 reads in 194.452 CPU sec, 6.313 real sec [M::mem_process_seqs] Processed 682438 reads in 136.005 CPU sec, 4.388 real sec sorting block 19, #reads = 1080923 mem = 536879352 peak = 1009590272 [M::process] read 682132 sequences (100000031 bp) in 3.013 CPU sec, 3.204 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282296, 4, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 203, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (215.04, 71.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 312, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (327.73, 152.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 231, 557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1289) [M::mem_pestat] mean and std.dev: (321.18, 178.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1655) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282219, 9, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 232, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (269.69, 114.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1163) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 313, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (329.32, 153.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 323, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (309.46, 104.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1175) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681842 sequences (100000063 bp) in 2.853 CPU sec, 3.608 real sec... [M::mem_process_seqs] Processed 682450 reads in 195.731 CPU sec, 6.503 real sec sorting block 20, #reads = 1080714 mem = 536881360 peak = 1009590272 [M::mem_process_seqs] Processed 682132 reads in 137.153 CPU sec, 4.552 real sec [M::process] read 681726 sequences (100000052 bp) in 3.038 CPU sec, 3.278 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 281691, 12, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 388, 626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1428) [M::mem_pestat] mean and std.dev: (346.71, 157.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 316, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (331.68, 154.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 70, 712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2082) [M::mem_pestat] mean and std.dev: (156.20, 236.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2767) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 157, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (199.53, 66.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 21, #reads = 1080598 mem = 536882823 peak = 1009590272 [M::process] read 681764 sequences (100000295 bp) in 3.173 CPU sec, 3.388 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 281116, 2, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 294, 1734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4818) [M::mem_pestat] mean and std.dev: (824.83, 1064.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 317, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (331.80, 153.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 234, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (41, 416) [M::mem_pestat] mean and std.dev: (206.23, 48.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681842 reads in 202.527 CPU sec, 6.620 real sec [M::mem_process_seqs] Processed 681726 reads in 138.194 CPU sec, 4.491 real sec [M::process] read 681298 sequences (100000104 bp) in 2.869 CPU sec, 3.186 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282267, 4, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 217, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 434) [M::mem_pestat] mean and std.dev: (189.05, 51.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 319, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (333.60, 155.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 345, 1147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3019) [M::mem_pestat] mean and std.dev: (517.61, 410.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3955) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 22, #reads = 1079207 mem = 536882039 peak = 1009590272 [M::process] read 681174 sequences (100000164 bp) in 2.833 CPU sec, 3.323 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282394, 3, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 284, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (225.23, 77.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 318, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (333.21, 153.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 257, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (288.69, 125.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1187) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681764 reads in 198.169 CPU sec, 6.491 real sec [M::process] read 680760 sequences (100000150 bp) in 2.827 CPU sec, 3.019 real sec... sorting block 23, #reads = 1079774 mem = 536879202 peak = 1009590272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 281625, 1, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 279, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1179) [M::mem_pestat] mean and std.dev: (260.40, 119.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 322, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (336.14, 155.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 232, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (224.73, 48.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681174 reads in 116.133 CPU sec, 3.709 real sec [M::process] read 681008 sequences (100000255 bp) in 2.568 CPU sec, 2.768 real sec... [M::mem_process_seqs] Processed 681298 reads in 324.791 CPU sec, 10.511 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281238, 9, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (266, 340, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1070) [M::mem_pestat] mean and std.dev: (369.61, 222.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1338) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 321, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (336.82, 155.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 342, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (276.04, 126.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 24, #reads = 1078809 mem = 536878118 peak = 1009590272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 281550, 7, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 254, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (234.68, 66.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 321, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (335.38, 154.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 303, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093) [M::mem_pestat] mean and std.dev: (350.67, 192.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1387) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680838 sequences (100000023 bp) in 2.791 CPU sec, 3.446 real sec... [M::mem_process_seqs] Processed 680760 reads in 226.111 CPU sec, 7.436 real sec [M::mem_process_seqs] Processed 681008 reads in 139.859 CPU sec, 4.671 real sec [M::process] read 680978 sequences (100000219 bp) in 3.200 CPU sec, 3.536 real sec... sorting block 25, #reads = 1079611 mem = 536879273 peak = 1009590272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 281741, 5, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 192, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1106) [M::mem_pestat] mean and std.dev: (264.52, 204.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 324, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (338.82, 156.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 282, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (274.20, 114.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1090) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 282216, 6, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 189, 687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1787) [M::mem_pestat] mean and std.dev: (185.41, 105.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2337) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 318, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (332.65, 152.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1093) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 303, 550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1186) [M::mem_pestat] mean and std.dev: (339.12, 163.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1504) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680994 sequences (100000074 bp) in 2.974 CPU sec, 3.464 real sec... [M::mem_process_seqs] Processed 680838 reads in 202.872 CPU sec, 6.626 real sec sorting block 26, #reads = 1078172 mem = 536879408 peak = 1009590272 [M::process] read 680428 sequences (100000223 bp) in 2.984 CPU sec, 3.419 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 281347, 6, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 296, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1111) [M::mem_pestat] mean and std.dev: (375.75, 194.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 321, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (336.24, 155.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 317, 694) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1716) [M::mem_pestat] mean and std.dev: (358.84, 215.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2227) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680994 reads in 119.941 CPU sec, 3.910 real sec [M::process] read 680284 sequences (100000180 bp) in 2.616 CPU sec, 3.001 real sec... [M::mem_process_seqs] Processed 680978 reads in 338.904 CPU sec, 11.066 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282401, 4, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 272, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (269.96, 133.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 323, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (338.09, 155.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1123) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 227, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966) [M::mem_pestat] mean and std.dev: (231.84, 108.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1233) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 27, #reads = 1078524 mem = 536880914 peak = 1009590272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 281254, 2, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 220, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (246.00, 106.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 322, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (337.15, 155.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 289, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (243.88, 98.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680616 sequences (100000225 bp) in 3.208 CPU sec, 3.542 real sec... [M::mem_process_seqs] Processed 680428 reads in 227.982 CPU sec, 7.485 real sec [M::mem_process_seqs] Processed 680284 reads in 140.625 CPU sec, 4.640 real sec sorting block 28, #reads = 1079485 mem = 536879156 peak = 1009590272 [M::process] read 681214 sequences (100000174 bp) in 3.183 CPU sec, 3.415 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 281932, 4, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 304, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974) [M::mem_pestat] mean and std.dev: (285.25, 136.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1235) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 321, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (337.29, 156.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 221, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (216.00, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282426, 10, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 196, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (194.17, 62.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 316, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (332.89, 155.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (722, 4838, 5597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15347) [M::mem_pestat] mean and std.dev: (3100.80, 2603.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20222) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 213, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (22, 397) [M::mem_pestat] mean and std.dev: (188.35, 43.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680640 sequences (100000059 bp) in 2.836 CPU sec, 3.308 real sec... [M::mem_process_seqs] Processed 680616 reads in 204.205 CPU sec, 6.697 real sec [M::mem_process_seqs] Processed 681214 reads in 144.628 CPU sec, 4.722 real sec sorting block 29, #reads = 1077902 mem = 536879686 peak = 1033265152 [M::process] read 681026 sequences (100000168 bp) in 3.026 CPU sec, 3.198 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 281527, 4, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 246, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (246.54, 111.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 321, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (336.21, 155.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 358, 1153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3035) [M::mem_pestat] mean and std.dev: (663.73, 704.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3976) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 30, #reads = 1077915 mem = 536880674 peak = 1033265152 [M::process] read 681104 sequences (100000198 bp) in 2.887 CPU sec, 3.560 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 281531, 5, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (191.87, 77.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 318, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (333.71, 154.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 188, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1349) [M::mem_pestat] mean and std.dev: (310.62, 268.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1752) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680640 reads in 202.590 CPU sec, 6.640 real sec [M::mem_process_seqs] Processed 681026 reads in 142.505 CPU sec, 4.652 real sec [M::process] read 681072 sequences (100000070 bp) in 3.389 CPU sec, 3.624 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282072, 2, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 389, 594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1472) [M::mem_pestat] mean and std.dev: (471.00, 426.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2176) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 318, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (334.14, 154.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 216, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (258.73, 127.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1085) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 31, #reads = 1077491 mem = 536878647 peak = 1033265152 [M::process] read 680842 sequences (100000271 bp) in 3.025 CPU sec, 3.401 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 281705, 8, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (256, 382, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039) [M::mem_pestat] mean and std.dev: (401.41, 196.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1300) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 316, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (332.94, 154.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 257, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (279.00, 124.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1030) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681104 reads in 200.776 CPU sec, 6.541 real sec [M::mem_process_seqs] Processed 681072 reads in 140.282 CPU sec, 4.555 real sec [M::process] read 680780 sequences (100000062 bp) in 2.760 CPU sec, 2.974 real sec... sorting block 32, #reads = 1076285 mem = 536887173 peak = 1033265152 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 281235, 1, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 217, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951) [M::mem_pestat] mean and std.dev: (232.59, 117.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1217) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 318, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (334.02, 154.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 206, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (235.31, 101.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680708 sequences (100000164 bp) in 2.981 CPU sec, 3.378 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 281485, 1, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 233, 953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2609) [M::mem_pestat] mean and std.dev: (455.52, 414.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 320, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (336.76, 156.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 275, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1121) [M::mem_pestat] mean and std.dev: (318.03, 195.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1434) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680842 reads in 202.838 CPU sec, 6.707 real sec [M::mem_process_seqs] Processed 680780 reads in 141.321 CPU sec, 4.673 real sec sorting block 33, #reads = 1077050 mem = 536881853 peak = 1056260096 [M::process] read 680550 sequences (100000023 bp) in 2.885 CPU sec, 3.177 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 281628, 6, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 311, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (290.62, 113.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 322, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (339.15, 158.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 335, 621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1441) [M::mem_pestat] mean and std.dev: (364.30, 216.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1851) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681400 sequences (100000099 bp) in 3.111 CPU sec, 3.708 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 281998, 6, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 198, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (193.48, 95.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 324, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (341.11, 160.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1150) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 273, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (267.53, 115.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680708 reads in 202.429 CPU sec, 6.691 real sec sorting block 34, #reads = 1076999 mem = 536877877 peak = 1064030208 [M::mem_process_seqs] Processed 680550 reads in 141.651 CPU sec, 4.671 real sec [M::process] read 684306 sequences (100000061 bp) in 2.856 CPU sec, 3.069 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 281603, 9, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 221, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (13, 458) [M::mem_pestat] mean and std.dev: (224.09, 64.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 319, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (336.34, 158.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 312, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (293.91, 168.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1163) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 35, #reads = 1077313 mem = 536878998 peak = 1070235648 [M::process] read 683606 sequences (100000231 bp) in 3.223 CPU sec, 3.630 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 283000, 1, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 296, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (280.56, 145.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 302, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (319.35, 151.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 238, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (262.78, 115.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681400 reads in 202.940 CPU sec, 6.638 real sec [M::mem_process_seqs] Processed 684306 reads in 140.421 CPU sec, 4.553 real sec [M::process] read 682718 sequences (100000132 bp) in 3.090 CPU sec, 3.268 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 282794, 1, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 234, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (237.91, 103.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 307, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (323.61, 152.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1090) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 261, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (287.59, 123.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 36, #reads = 1076033 mem = 536879219 peak = 1070235648 [M::process] read 682556 sequences (100000013 bp) in 3.251 CPU sec, 3.519 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 281949, 5, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (205.64, 106.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 310, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (326.03, 152.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1089) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 290, 598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1418) [M::mem_pestat] mean and std.dev: (384.15, 260.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1828) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683606 reads in 197.427 CPU sec, 6.418 real sec [M::mem_process_seqs] Processed 682718 reads in 138.680 CPU sec, 4.476 real sec [M::process] read 682192 sequences (100000203 bp) in 3.081 CPU sec, 3.378 real sec... sorting block 37, #reads = 1081114 mem = 536880654 peak = 1070235648 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282308, 3, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (229, 279, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (47, 502) [M::mem_pestat] mean and std.dev: (251.04, 81.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 311, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (326.60, 152.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 408, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1099) [M::mem_pestat] mean and std.dev: (354.83, 147.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1395) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282805, 3, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 272, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (237.22, 116.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1169) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 314, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (329.72, 153.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1100) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 297, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (263.28, 74.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681778 sequences (100000113 bp) in 3.344 CPU sec, 3.742 real sec... [M::mem_process_seqs] Processed 682556 reads in 198.535 CPU sec, 6.474 real sec [M::mem_process_seqs] Processed 682192 reads in 141.973 CPU sec, 4.574 real sec sorting block 38, #reads = 1079819 mem = 536879577 peak = 1070235648 [M::process] read 681748 sequences (100000051 bp) in 3.070 CPU sec, 3.415 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282016, 6, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 246, 540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1282) [M::mem_pestat] mean and std.dev: (367.14, 300.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 315, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (330.85, 153.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 308, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (299.72, 135.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282133, 5, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 215, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (244.18, 107.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 318, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (333.03, 154.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 209, 917) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2381) [M::mem_pestat] mean and std.dev: (538.06, 654.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3158) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681702 sequences (100000070 bp) in 2.966 CPU sec, 3.278 real sec... sorting block 39, #reads = 1079587 mem = 536879355 peak = 1070235648 [M::mem_process_seqs] Processed 681778 reads in 197.410 CPU sec, 6.507 real sec [M::mem_process_seqs] Processed 681748 reads in 140.620 CPU sec, 4.611 real sec [M::process] read 681506 sequences (100000074 bp) in 2.871 CPU sec, 3.321 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 282024, 2, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 233, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (226.95, 88.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 319, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (334.46, 156.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 384, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1064) [M::mem_pestat] mean and std.dev: (320.76, 173.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1352) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 40, #reads = 1079415 mem = 536878943 peak = 1070235648 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 281489, 6, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 228, 564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1436) [M::mem_pestat] mean and std.dev: (295.25, 193.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1872) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 321, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (337.15, 157.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 203, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (229.23, 85.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680948 sequences (100000126 bp) in 3.182 CPU sec, 3.494 real sec... [M::mem_process_seqs] Processed 681702 reads in 194.355 CPU sec, 6.342 real sec [M::mem_process_seqs] Processed 681506 reads in 135.475 CPU sec, 4.334 real sec [M::process] read 680616 sequences (100000291 bp) in 2.849 CPU sec, 3.120 real sec... sorting block 41, #reads = 1078918 mem = 536877843 peak = 1070235648 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282074, 15, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 275, 921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2439) [M::mem_pestat] mean and std.dev: (532.11, 533.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3198) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 323, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (338.42, 157.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 334, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940) [M::mem_pestat] mean and std.dev: (216.93, 172.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1243) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 291, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (272.53, 98.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680780 sequences (100000227 bp) in 3.027 CPU sec, 3.371 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 281344, 3, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 261, 1280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3540) [M::mem_pestat] mean and std.dev: (863.96, 907.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 322, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (337.72, 156.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 253, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (259.34, 104.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680948 reads in 198.025 CPU sec, 6.500 real sec [M::mem_process_seqs] Processed 680616 reads in 140.493 CPU sec, 4.610 real sec sorting block 42, #reads = 1078939 mem = 536877984 peak = 1070235648 [M::process] read 680584 sequences (100000097 bp) in 2.744 CPU sec, 2.883 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 281975, 6, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 218, 1130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3050) [M::mem_pestat] mean and std.dev: (442.94, 396.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4010) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 324, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (339.03, 157.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (133, 264, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (225.33, 87.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680460 sequences (100000185 bp) in 2.996 CPU sec, 3.287 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 281567, 11, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 397, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1246) [M::mem_pestat] mean and std.dev: (360.17, 199.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 324, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (339.09, 156.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (743, 3659, 5641) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15437) [M::mem_pestat] mean and std.dev: (3115.64, 2212.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20335) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 227, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (244.29, 133.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1069) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680780 reads in 198.480 CPU sec, 6.400 real sec sorting block 43, #reads = 1078714 mem = 536884190 peak = 1095868416 [M::process] read 680280 sequences (100000196 bp) in 2.848 CPU sec, 2.909 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 281438, 9, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 259, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1109) [M::mem_pestat] mean and std.dev: (273.20, 144.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 325, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (340.43, 157.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 312, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (260.32, 87.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680460 reads in 113.308 CPU sec, 3.638 real sec [M::process] read 680710 sequences (100000084 bp) in 2.326 CPU sec, 2.434 real sec... sorting block 44, #reads = 1078723 mem = 536880599 peak = 1095868416 [M::mem_process_seqs] Processed 680584 reads in 308.342 CPU sec, 9.933 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 281245, 3, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (227, 378, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (293.85, 120.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 324, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (339.46, 156.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (281, 355, 581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1181) [M::mem_pestat] mean and std.dev: (361.53, 142.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1481) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680726 sequences (100000193 bp) in 3.095 CPU sec, 3.456 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 281599, 12, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 255, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (229.03, 93.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 994) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 319, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (335.18, 155.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (329, 1015, 8324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 24314) [M::mem_pestat] mean and std.dev: (3164.33, 3660.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 32309) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (248, 367, 1325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3479) [M::mem_pestat] mean and std.dev: (732.77, 755.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4556) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680280 reads in 226.698 CPU sec, 7.363 real sec [M::mem_process_seqs] Processed 680710 reads in 139.115 CPU sec, 4.494 real sec sorting block 45, #reads = 1079776 mem = 536880800 peak = 1095868416 [M::process] read 680612 sequences (100000060 bp) in 2.817 CPU sec, 3.083 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 281870, 3, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 304, 599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1345) [M::mem_pestat] mean and std.dev: (431.86, 269.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 320, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (336.61, 156.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 252, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949) [M::mem_pestat] mean and std.dev: (285.11, 123.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1193) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 46, #reads = 1078028 mem = 536881003 peak = 1095868416 [M::process] read 680796 sequences (100000118 bp) in 2.763 CPU sec, 3.137 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 281047, 3, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 262, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (219.44, 65.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 318, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (335.25, 155.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 232, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1170) [M::mem_pestat] mean and std.dev: (232.24, 102.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1498) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680726 reads in 201.082 CPU sec, 6.558 real sec [M::mem_process_seqs] Processed 680612 reads in 143.234 CPU sec, 4.618 real sec [M::process] read 680992 sequences (100000255 bp) in 2.708 CPU sec, 2.792 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 281491, 6, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 352, 603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1429) [M::mem_pestat] mean and std.dev: (428.89, 280.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1842) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 318, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (334.62, 155.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 328, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986) [M::mem_pestat] mean and std.dev: (299.56, 155.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1250) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 47, #reads = 1079045 mem = 536878953 peak = 1095868416 [M::process] read 680900 sequences (100000286 bp) in 3.193 CPU sec, 3.551 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 282021, 3, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 211, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (194.00, 76.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 315, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (332.73, 155.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 221, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (221.68, 70.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680796 reads in 202.863 CPU sec, 6.554 real sec [M::mem_process_seqs] Processed 680992 reads in 143.766 CPU sec, 4.609 real sec [M::process] read 681004 sequences (100000037 bp) in 2.620 CPU sec, 2.940 real sec... sorting block 48, #reads = 1078432 mem = 536877743 peak = 1095868416 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 281188, 7, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 197, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (201.84, 77.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 317, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (332.60, 154.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 244, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1139) [M::mem_pestat] mean and std.dev: (344.11, 205.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1460) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680786 sequences (100000017 bp) in 2.970 CPU sec, 3.259 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 282248, 5, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 270, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (230.60, 74.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 315, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (332.01, 154.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 272, 608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1366) [M::mem_pestat] mean and std.dev: (421.00, 308.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1745) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680900 reads in 199.795 CPU sec, 6.442 real sec [M::mem_process_seqs] Processed 681004 reads in 140.368 CPU sec, 4.485 real sec [M::process] read 681238 sequences (100000269 bp) in 2.638 CPU sec, 2.707 real sec... sorting block 49, #reads = 1078139 mem = 536886588 peak = 1095868416 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281655, 8, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 273, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (258.13, 95.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 316, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (332.87, 154.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 256, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (214.04, 65.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681208 sequences (100000157 bp) in 3.277 CPU sec, 3.618 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281528, 4, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 218, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (187.81, 67.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 314, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (331.08, 154.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 345, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1072) [M::mem_pestat] mean and std.dev: (377.58, 193.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1360) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680786 reads in 200.336 CPU sec, 6.560 real sec [M::mem_process_seqs] Processed 681238 reads in 138.125 CPU sec, 4.517 real sec sorting block 50, #reads = 1077083 mem = 536880677 peak = 1095868416 [M::process] read 680540 sequences (100000148 bp) in 2.663 CPU sec, 2.984 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 281466, 9, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 257, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1100) [M::mem_pestat] mean and std.dev: (312.76, 177.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 317, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (334.27, 156.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (266, 383, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (340.43, 138.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 51, #reads = 1077307 mem = 536880015 peak = 1100103680 [M::process] read 680492 sequences (100000062 bp) in 3.338 CPU sec, 3.821 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 281747, 4, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 244, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (245.28, 102.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 320, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (336.53, 156.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 288, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947) [M::mem_pestat] mean and std.dev: (277.15, 144.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1211) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681208 reads in 198.668 CPU sec, 6.447 real sec [M::mem_process_seqs] Processed 680540 reads in 137.909 CPU sec, 4.441 real sec [M::process] read 680396 sequences (100000004 bp) in 2.985 CPU sec, 3.159 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 281499, 3, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 187, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (208.12, 100.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 323, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (340.50, 159.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1142) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 315, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (331.77, 140.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 52, #reads = 1077195 mem = 536876927 peak = 1100103680 [M::process] read 680102 sequences (100000078 bp) in 3.291 CPU sec, 3.676 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 281161, 6, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 223, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (206.39, 54.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 324, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (342.07, 160.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 267, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020) [M::mem_pestat] mean and std.dev: (282.16, 129.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1302) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680492 reads in 198.313 CPU sec, 6.444 real sec [M::mem_process_seqs] Processed 680396 reads in 141.462 CPU sec, 4.538 real sec [M::process] read 683710 sequences (100000084 bp) in 2.913 CPU sec, 3.077 real sec... sorting block 53, #reads = 1077569 mem = 536882190 peak = 1100103680 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 281612, 8, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 306, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (282.96, 126.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 328, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (345.61, 163.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1164) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 314, 554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1316) [M::mem_pestat] mean and std.dev: (312.74, 219.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1697) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683524 sequences (100000161 bp) in 3.270 CPU sec, 3.700 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282729, 12, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 206, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (197.24, 55.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 306, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (324.58, 154.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (657, 657, 657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (657, 657) [M::mem_pestat] mean and std.dev: (657.00, 0.00) [M::mem_pestat] low and high boundaries for proper pairs: (657, 657) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 210, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (218.96, 85.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680102 reads in 202.432 CPU sec, 6.552 real sec [M::mem_process_seqs] Processed 683710 reads in 145.515 CPU sec, 4.659 real sec sorting block 54, #reads = 1077284 mem = 536877723 peak = 1100103680 [M::process] read 682914 sequences (100000083 bp) in 3.034 CPU sec, 3.114 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282193, 8, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 183, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (215.92, 82.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 306, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (324.67, 154.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (244, 295, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (299.91, 90.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682648 sequences (100000151 bp) in 3.450 CPU sec, 3.931 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282056, 6, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 172, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (204.62, 96.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 312, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (330.27, 157.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 184, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (81, 281) [M::mem_pestat] mean and std.dev: (158.14, 33.99) [M::mem_pestat] low and high boundaries for proper pairs: (22, 321) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683524 reads in 200.504 CPU sec, 6.499 real sec sorting block 55, #reads = 1078672 mem = 536878101 peak = 1100103680 [M::mem_process_seqs] Processed 682914 reads in 141.582 CPU sec, 4.544 real sec [M::process] read 681816 sequences (100000292 bp) in 2.860 CPU sec, 3.117 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 282436, 12, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 210, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (289.00, 170.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 314, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (331.24, 156.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (233, 290, 2192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6110) [M::mem_pestat] mean and std.dev: (1263.92, 1428.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8069) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 249, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (255.90, 128.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1017) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 56, #reads = 1080847 mem = 536880587 peak = 1142210560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 281922, 2, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 206, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (229.78, 99.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 314, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (331.32, 155.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 296, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1067) [M::mem_pestat] mean and std.dev: (327.33, 178.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1357) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681822 sequences (100000253 bp) in 3.147 CPU sec, 3.532 real sec... [M::mem_process_seqs] Processed 682648 reads in 194.724 CPU sec, 6.315 real sec [M::mem_process_seqs] Processed 681816 reads in 136.387 CPU sec, 4.367 real sec [M::process] read 681350 sequences (100000285 bp) in 3.000 CPU sec, 3.112 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 281559, 2, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 282, 573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1351) [M::mem_pestat] mean and std.dev: (328.00, 180.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 315, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (331.66, 155.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 213, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (229.52, 107.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 57, #reads = 1081045 mem = 536879666 peak = 1142210560 [M::process] read 681348 sequences (100000026 bp) in 2.859 CPU sec, 3.118 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 281519, 4, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 224, 693) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1803) [M::mem_pestat] mean and std.dev: (267.89, 254.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2358) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 320, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (336.36, 157.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 236, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (230.00, 87.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681822 reads in 195.445 CPU sec, 6.364 real sec [M::mem_process_seqs] Processed 681350 reads in 138.728 CPU sec, 4.525 real sec [M::process] read 681324 sequences (100000290 bp) in 2.551 CPU sec, 2.681 real sec... sorting block 58, #reads = 1079270 mem = 536879886 peak = 1142210560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 281493, 5, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 216, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (204.45, 89.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1062) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 321, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (337.52, 158.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 207, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (219.94, 98.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680896 sequences (100000170 bp) in 3.233 CPU sec, 3.426 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281863, 4, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 347, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (341.19, 150.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1143) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 323, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (339.34, 159.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 258, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (15, 500) [M::mem_pestat] mean and std.dev: (225.13, 62.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681348 reads in 199.257 CPU sec, 6.404 real sec [M::process] read 680768 sequences (100000135 bp) in 2.658 CPU sec, 2.714 real sec... sorting block 59, #reads = 1079696 mem = 536883324 peak = 1142210560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 281189, 9, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 393, 800) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2044) [M::mem_pestat] mean and std.dev: (329.38, 210.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 323, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (339.69, 159.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 407, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1103) [M::mem_pestat] mean and std.dev: (424.14, 228.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1391) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680896 reads in 116.251 CPU sec, 3.714 real sec [M::process] read 680570 sequences (100000265 bp) in 2.656 CPU sec, 2.665 real sec... [M::mem_process_seqs] Processed 681324 reads in 300.703 CPU sec, 9.614 real sec sorting block 60, #reads = 1079676 mem = 536878570 peak = 1142210560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281419, 3, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 248, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (263.04, 95.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 326, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (342.25, 160.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1151) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 177, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (204.57, 82.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680956 sequences (100000248 bp) in 3.310 CPU sec, 3.786 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 281051, 4, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 169, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (220.88, 126.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 325, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (340.50, 158.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 214, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (22, 452) [M::mem_pestat] mean and std.dev: (215.44, 52.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680768 reads in 224.139 CPU sec, 7.235 real sec [M::mem_process_seqs] Processed 680570 reads in 140.874 CPU sec, 4.539 real sec sorting block 61, #reads = 1079772 mem = 536883537 peak = 1142210560 [M::process] read 680662 sequences (100000002 bp) in 3.057 CPU sec, 3.157 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 281741, 12, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 212, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (240.25, 135.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 323, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (339.43, 158.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (274, 1075, 1075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2677) [M::mem_pestat] mean and std.dev: (783.00, 586.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3478) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 300, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (292.71, 107.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680056 sequences (100000163 bp) in 2.997 CPU sec, 3.305 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281086, 4, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 203, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (233.33, 81.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 324, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (338.99, 157.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (254, 313, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (84, 509) [M::mem_pestat] mean and std.dev: (288.77, 87.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680956 reads in 198.610 CPU sec, 6.445 real sec sorting block 62, #reads = 1079041 mem = 536878560 peak = 1142210560 [M::mem_process_seqs] Processed 680662 reads in 139.518 CPU sec, 4.501 real sec [M::process] read 680098 sequences (100000299 bp) in 2.782 CPU sec, 2.916 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281660, 1, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 270, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (265.58, 126.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 324, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (339.12, 156.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (270, 347, 678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1494) [M::mem_pestat] mean and std.dev: (380.12, 174.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1902) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 63, #reads = 1078934 mem = 536879933 peak = 1142210560 [M::process] read 681038 sequences (100000230 bp) in 3.062 CPU sec, 3.443 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 281367, 6, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 255, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (252.20, 111.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (217, 326, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (341.64, 157.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (244, 322, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (289.20, 123.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680056 reads in 201.159 CPU sec, 6.547 real sec [M::mem_process_seqs] Processed 680098 reads in 138.956 CPU sec, 4.496 real sec [M::process] read 681560 sequences (100000131 bp) in 2.747 CPU sec, 2.941 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 281820, 7, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 267, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (225.09, 80.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 318, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (334.45, 155.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 353, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1086) [M::mem_pestat] mean and std.dev: (341.57, 163.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1378) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 64, #reads = 1077530 mem = 536878825 peak = 1142210560 [M::process] read 681308 sequences (100000207 bp) in 3.158 CPU sec, 3.431 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282135, 5, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 210, 635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1665) [M::mem_pestat] mean and std.dev: (388.21, 363.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2180) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 312, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (329.88, 153.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1097) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 313, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (282.06, 76.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1190) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681038 reads in 201.569 CPU sec, 6.510 real sec [M::mem_process_seqs] Processed 681560 reads in 140.166 CPU sec, 4.486 real sec [M::process] read 681206 sequences (100000147 bp) in 3.073 CPU sec, 3.180 real sec... sorting block 65, #reads = 1078068 mem = 536878645 peak = 1142210560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 281472, 3, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 154, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (177.68, 75.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 313, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (329.87, 153.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1097) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 332, 679) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1567) [M::mem_pestat] mean and std.dev: (351.08, 260.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2011) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681144 sequences (100000164 bp) in 3.436 CPU sec, 3.837 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 281307, 5, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (222, 254, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (269.76, 91.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 315, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (331.52, 153.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (121, 172, 655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1723) [M::mem_pestat] mean and std.dev: (303.24, 279.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2257) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681308 reads in 201.689 CPU sec, 6.486 real sec [M::mem_process_seqs] Processed 681206 reads in 142.502 CPU sec, 4.545 real sec sorting block 66, #reads = 1079044 mem = 536879686 peak = 1142210560 [M::process] read 681262 sequences (100000055 bp) in 2.722 CPU sec, 2.802 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 281502, 5, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 299, 1361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3705) [M::mem_pestat] mean and std.dev: (606.06, 664.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4877) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 315, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (331.56, 153.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (265, 302, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (321.87, 129.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 281647, 3, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 256, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1138) [M::mem_pestat] mean and std.dev: (231.56, 131.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 312, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (329.26, 152.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1082) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 377, 499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1141) [M::mem_pestat] mean and std.dev: (366.78, 240.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1462) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681376 sequences (100000157 bp) in 4.040 CPU sec, 4.673 real sec... [M::mem_process_seqs] Processed 681144 reads in 202.730 CPU sec, 6.564 real sec sorting block 67, #reads = 1077762 mem = 536882142 peak = 1142210560 [M::mem_process_seqs] Processed 681262 reads in 143.227 CPU sec, 4.613 real sec [M::process] read 681062 sequences (100000250 bp) in 3.468 CPU sec, 3.806 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 281319, 6, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 277, 593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1393) [M::mem_pestat] mean and std.dev: (280.42, 153.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 313, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (329.91, 152.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (294, 390, 550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062) [M::mem_pestat] mean and std.dev: (373.81, 142.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1318) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 281812, 8, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 232, 549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1221) [M::mem_pestat] mean and std.dev: (277.39, 144.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 316, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (332.01, 153.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 235, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (266.33, 113.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 68, #reads = 1077415 mem = 536879111 peak = 1142210560 [M::mem_process_seqs] Processed 681376 reads in 203.581 CPU sec, 6.654 real sec [M::process] read 680666 sequences (100000240 bp) in 4.217 CPU sec, 4.862 real sec... [M::mem_process_seqs] Processed 681062 reads in 144.177 CPU sec, 4.689 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 281819, 8, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 310, 535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1311) [M::mem_pestat] mean and std.dev: (308.92, 221.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 318, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (334.55, 155.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (305, 611, 1290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3260) [M::mem_pestat] mean and std.dev: (624.92, 539.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4245) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680666 reads in 111.983 CPU sec, 3.789 real sec [M::process] read 680932 sequences (100000296 bp) in 3.839 CPU sec, 4.446 real sec... sorting block 69, #reads = 1077852 mem = 536881169 peak = 1142210560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282247, 10, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 212, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (196.54, 68.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 317, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (333.55, 154.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (685, 2909, 2909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7357) [M::mem_pestat] mean and std.dev: (2041.89, 2033.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10177) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 286, 1038) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2662) [M::mem_pestat] mean and std.dev: (594.44, 516.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3474) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680932 reads in 114.696 CPU sec, 3.896 real sec [M::process] read 680688 sequences (100000299 bp) in 3.739 CPU sec, 4.064 real sec... sorting block 70, #reads = 1078085 mem = 536878740 peak = 1142210560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 281516, 3, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 194, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (63, 358) [M::mem_pestat] mean and std.dev: (192.75, 57.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 320, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (337.31, 157.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 314, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (301.22, 152.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1091) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680688 reads in 113.824 CPU sec, 3.839 real sec [M::process] read 679408 sequences (100000204 bp) in 3.623 CPU sec, 4.160 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 281292, 7, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 192, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (214.05, 89.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (220, 331, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (346.42, 159.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 246, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (229.20, 73.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680542 sequences (100000077 bp) in 3.156 CPU sec, 3.350 real sec... [M::mem_process_seqs] Processed 679408 reads in 116.391 CPU sec, 3.931 real sec sorting block 71, #reads = 1079180 mem = 536878889 peak = 1142210560 [M::process] read 681272 sequences (100000145 bp) in 2.977 CPU sec, 3.199 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 281123, 3, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 230, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1231) [M::mem_pestat] mean and std.dev: (264.76, 169.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 320, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (337.98, 158.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 237, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (247.48, 109.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1064) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281929, 5, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 216, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (218.26, 102.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 319, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (337.65, 160.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1143) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 223, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (230.80, 114.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688520 sequences (100000236 bp) in 3.260 CPU sec, 3.800 real sec... sorting block 72, #reads = 1076952 mem = 536876455 peak = 1142210560 [M::mem_process_seqs] Processed 680542 reads in 202.037 CPU sec, 6.645 real sec [M::mem_process_seqs] Processed 681272 reads in 144.637 CPU sec, 4.734 real sec [M::process] read 686686 sequences (100000294 bp) in 3.283 CPU sec, 3.496 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 284830, 5, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 211, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011) [M::mem_pestat] mean and std.dev: (248.28, 136.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1293) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 278, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (303.80, 150.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1063) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 229, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (230.26, 85.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 73, #reads = 1075930 mem = 536880078 peak = 1142210560 [M::process] read 686006 sequences (100000147 bp) in 3.109 CPU sec, 3.546 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 283930, 6, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 202, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (184.65, 78.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 290, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (312.75, 152.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1090) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 229, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (222.23, 73.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688520 reads in 227.268 CPU sec, 7.485 real sec [M::mem_process_seqs] Processed 686686 reads in 156.446 CPU sec, 5.165 real sec [M::process] read 684994 sequences (100000085 bp) in 3.045 CPU sec, 3.217 real sec... sorting block 74, #reads = 1077003 mem = 536879621 peak = 1142210560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283835, 6, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 331, 549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1331) [M::mem_pestat] mean and std.dev: (360.05, 283.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 295, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (316.89, 153.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 271, 602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1386) [M::mem_pestat] mean and std.dev: (393.92, 222.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1778) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684202 sequences (100000279 bp) in 3.177 CPU sec, 3.583 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 283814, 12, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 221, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (217.30, 108.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 300, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (321.73, 155.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (224, 986, 1058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2726) [M::mem_pestat] mean and std.dev: (640.27, 465.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3560) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 246, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (292.56, 146.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686006 reads in 218.762 CPU sec, 7.120 real sec [M::mem_process_seqs] Processed 684994 reads in 149.419 CPU sec, 4.889 real sec [M::process] read 683478 sequences (100000138 bp) in 2.622 CPU sec, 2.868 real sec... sorting block 75, #reads = 1077841 mem = 536876881 peak = 1142210560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 283178, 4, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 242, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (265.85, 120.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 304, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (324.36, 155.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1123) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (331, 355, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (269, 424) [M::mem_pestat] mean and std.dev: (346.68, 23.53) [M::mem_pestat] low and high boundaries for proper pairs: (238, 455) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682696 sequences (100000182 bp) in 3.167 CPU sec, 3.937 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282909, 6, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 276, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (277.58, 106.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 312, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (330.29, 157.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1143) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 211, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936) [M::mem_pestat] mean and std.dev: (232.57, 96.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1185) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684202 reads in 215.015 CPU sec, 6.994 real sec sorting block 76, #reads = 1078124 mem = 536877121 peak = 1147604992 [M::mem_process_seqs] Processed 683478 reads in 150.683 CPU sec, 4.823 real sec [M::process] read 682326 sequences (100000143 bp) in 2.684 CPU sec, 2.761 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282518, 3, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 245, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (245.24, 92.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 316, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (334.46, 159.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (278, 422, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968) [M::mem_pestat] mean and std.dev: (360.53, 115.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1198) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 77, #reads = 1077867 mem = 536877855 peak = 1147871232 [M::process] read 682674 sequences (100000195 bp) in 3.075 CPU sec, 3.614 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282850, 1, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 228, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (227.21, 120.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 320, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (336.88, 159.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1167) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (253, 277, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (311.10, 125.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682696 reads in 208.560 CPU sec, 6.789 real sec [M::mem_process_seqs] Processed 682326 reads in 146.271 CPU sec, 4.728 real sec [M::process] read 682532 sequences (100000027 bp) in 2.706 CPU sec, 2.922 real sec... sorting block 78, #reads = 1078332 mem = 536879410 peak = 1147871232 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 282588, 10, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 223, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (257.14, 132.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 318, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923) [M::mem_pestat] mean and std.dev: (335.22, 159.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1162) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (195, 1249, 1249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3357) [M::mem_pestat] mean and std.dev: (802.40, 572.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4411) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 328, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1070) [M::mem_pestat] mean and std.dev: (317.09, 165.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1360) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682390 sequences (100000234 bp) in 3.271 CPU sec, 3.604 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 283242, 2, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 301, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (317.12, 153.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 317, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (334.12, 157.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1158) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (254, 307, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (100, 485) [M::mem_pestat] mean and std.dev: (278.19, 69.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682674 reads in 215.632 CPU sec, 6.950 real sec [M::process] read 682338 sequences (100000131 bp) in 2.639 CPU sec, 2.784 real sec... [M::mem_process_seqs] Processed 682532 reads in 155.272 CPU sec, 4.970 real sec sorting block 79, #reads = 1078032 mem = 536880134 peak = 1147871232 [M::process] read 682480 sequences (100000230 bp) in 3.243 CPU sec, 3.539 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282327, 4, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 376, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1193) [M::mem_pestat] mean and std.dev: (352.50, 199.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 317, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (334.02, 157.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 268, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (258.21, 99.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682598 sequences (100000043 bp) in 2.494 CPU sec, 2.564 real sec... [M::mem_process_seqs] Processed 682338 reads in 126.226 CPU sec, 4.016 real sec sorting block 80, #reads = 1075390 mem = 536880343 peak = 1147871232 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 282876, 5, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 175, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (166.67, 42.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 318, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (333.90, 156.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1150) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 241, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (279.72, 141.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282440, 5, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 261, 556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1348) [M::mem_pestat] mean and std.dev: (304.12, 177.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 317, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (333.61, 156.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 254, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (271.22, 158.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1158) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682390 reads in 341.523 CPU sec, 11.036 real sec [M::process] read 682314 sequences (100000261 bp) in 2.670 CPU sec, 2.725 real sec... [M::mem_process_seqs] Processed 682480 reads in 181.899 CPU sec, 5.917 real sec sorting block 81, #reads = 1074873 mem = 536877713 peak = 1147871232 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 282826, 14, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 294, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (294.92, 118.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 316, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (332.30, 156.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1142) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 899, 994) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2896) [M::mem_pestat] mean and std.dev: (731.93, 654.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3847) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 275, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (279.15, 111.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681924 sequences (100000177 bp) in 3.190 CPU sec, 3.663 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 281748, 2, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 319, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (303.32, 152.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 314, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (331.90, 156.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1138) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 301, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (39, 549) [M::mem_pestat] mean and std.dev: (272.93, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682598 reads in 216.468 CPU sec, 7.023 real sec [M::process] read 681614 sequences (100000278 bp) in 2.640 CPU sec, 2.653 real sec... [M::mem_process_seqs] Processed 682314 reads in 151.235 CPU sec, 4.873 real sec sorting block 82, #reads = 1076008 mem = 536877656 peak = 1147871232 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282298, 6, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 258, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030) [M::mem_pestat] mean and std.dev: (281.65, 138.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1313) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 317, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (333.95, 156.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 207, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (243.44, 123.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681680 sequences (100000038 bp) in 3.082 CPU sec, 3.366 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 282406, 5, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 236, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (224.93, 100.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 320, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (335.52, 156.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 391, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992) [M::mem_pestat] mean and std.dev: (295.25, 127.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1263) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681924 reads in 219.702 CPU sec, 7.105 real sec [M::process] read 681318 sequences (100000018 bp) in 2.352 CPU sec, 2.459 real sec... sorting block 83, #reads = 1076698 mem = 536879257 peak = 1147871232 [M::mem_process_seqs] Processed 681614 reads in 151.672 CPU sec, 4.903 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 282296, 1, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 227, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (293.04, 152.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 320, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (335.96, 157.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 312, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (310.21, 125.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 84, #reads = 1074825 mem = 536879623 peak = 1157668864 [M::process] read 681580 sequences (100000162 bp) in 3.576 CPU sec, 3.860 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 281573, 11, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 279, 635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1573) [M::mem_pestat] mean and std.dev: (352.04, 271.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2042) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 321, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (338.21, 158.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1166) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (306, 379, 1858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4962) [M::mem_pestat] mean and std.dev: (1269.09, 1297.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6514) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (349, 432, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (63, 778) [M::mem_pestat] mean and std.dev: (376.07, 126.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681680 reads in 222.407 CPU sec, 7.189 real sec [M::process] read 681308 sequences (100000280 bp) in 2.495 CPU sec, 2.561 real sec... [M::mem_process_seqs] Processed 681318 reads in 153.121 CPU sec, 4.949 real sec sorting block 85, #reads = 1075168 mem = 536878079 peak = 1157668864 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282225, 7, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 196, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992) [M::mem_pestat] mean and std.dev: (261.48, 205.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1271) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 319, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (336.18, 157.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 263, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (261.07, 95.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680752 sequences (100000089 bp) in 3.509 CPU sec, 3.901 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 282115, 4, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 238, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (269.08, 123.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1068) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 319, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (335.44, 156.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (260, 311, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (316.13, 117.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681580 reads in 224.508 CPU sec, 7.271 real sec [M::process] read 681064 sequences (100000124 bp) in 2.601 CPU sec, 2.830 real sec... [M::mem_process_seqs] Processed 681308 reads in 155.099 CPU sec, 5.023 real sec sorting block 86, #reads = 1073426 mem = 536879440 peak = 1157668864 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 281203, 9, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 262, 549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1273) [M::mem_pestat] mean and std.dev: (316.97, 221.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 324, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (339.17, 157.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 261, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (279.83, 151.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680922 sequences (100000249 bp) in 3.498 CPU sec, 3.799 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282198, 6, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 205, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (261.81, 143.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 323, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923) [M::mem_pestat] mean and std.dev: (339.35, 158.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 222, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (229.72, 102.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680296 sequences (100000132 bp) in 2.489 CPU sec, 2.497 real sec... [M::mem_process_seqs] Processed 680752 reads in 221.501 CPU sec, 7.130 real sec sorting block 87, #reads = 1073866 mem = 536879643 peak = 1157668864 [M::mem_process_seqs] Processed 681064 reads in 153.077 CPU sec, 4.952 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281662, 11, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (249.07, 128.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1063) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 323, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923) [M::mem_pestat] mean and std.dev: (339.94, 159.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (571, 762, 1914) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4600) [M::mem_pestat] mean and std.dev: (1287.00, 938.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5943) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 276, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (240.86, 120.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1000) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 88, #reads = 1074697 mem = 536878731 peak = 1166786560 [M::process] read 680538 sequences (100000058 bp) in 3.567 CPU sec, 3.939 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281715, 2, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 193, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (198.86, 69.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 327, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (344.84, 161.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1175) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 286, 554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1226) [M::mem_pestat] mean and std.dev: (316.76, 165.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1562) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680922 reads in 222.414 CPU sec, 7.192 real sec [M::process] read 688666 sequences (100000250 bp) in 2.463 CPU sec, 2.525 real sec... [M::mem_process_seqs] Processed 680296 reads in 155.067 CPU sec, 5.041 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281859, 7, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 226, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (284.67, 178.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1022) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 327, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (344.13, 161.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1167) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 265, 620) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1478) [M::mem_pestat] mean and std.dev: (370.40, 235.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1907) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 89, #reads = 1073537 mem = 536881207 peak = 1166786560 [M::process] read 688972 sequences (100000107 bp) in 3.761 CPU sec, 4.167 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 285102, 3, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 290, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (286.29, 156.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1075) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 280, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841) [M::mem_pestat] mean and std.dev: (303.69, 149.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 215, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993) [M::mem_pestat] mean and std.dev: (297.48, 172.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1267) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680538 reads in 229.936 CPU sec, 7.406 real sec [M::process] read 687346 sequences (100000111 bp) in 2.692 CPU sec, 2.715 real sec... [M::mem_process_seqs] Processed 688666 reads in 170.171 CPU sec, 5.444 real sec sorting block 90, #reads = 1074630 mem = 536879752 peak = 1166786560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 284677, 5, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 357, 824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2154) [M::mem_pestat] mean and std.dev: (421.78, 291.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2819) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 278, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (300.74, 145.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 246, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923) [M::mem_pestat] mean and std.dev: (286.96, 161.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1177) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686774 sequences (100000113 bp) in 3.108 CPU sec, 3.346 real sec... [M::process] read 686470 sequences (100000267 bp) in 2.597 CPU sec, 2.603 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 284922, 6, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 270, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1092) [M::mem_pestat] mean and std.dev: (277.60, 156.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 287, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (307.18, 147.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (141, 266, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1116) [M::mem_pestat] mean and std.dev: (352.26, 270.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1441) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688972 reads in 240.767 CPU sec, 7.709 real sec [M::mem_process_seqs] Processed 687346 reads in 164.806 CPU sec, 5.280 real sec sorting block 91, #reads = 1073171 mem = 536879215 peak = 1166786560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283908, 5, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 325, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (285.12, 132.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 290, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844) [M::mem_pestat] mean and std.dev: (308.65, 147.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1062) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 259, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (269.89, 117.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686126 sequences (100000221 bp) in 3.638 CPU sec, 4.165 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 284017, 5, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 286, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (226.68, 88.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 292, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846) [M::mem_pestat] mean and std.dev: (309.80, 146.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1064) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 285, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (290.88, 128.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685616 sequences (100000270 bp) in 2.601 CPU sec, 2.607 real sec... [M::mem_process_seqs] Processed 686774 reads in 239.644 CPU sec, 7.721 real sec sorting block 92, #reads = 1074227 mem = 536883648 peak = 1166786560 [M::mem_process_seqs] Processed 686470 reads in 165.283 CPU sec, 5.345 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 284709, 2, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 280, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (276.73, 120.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1009) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 292, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846) [M::mem_pestat] mean and std.dev: (310.60, 146.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1064) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 403, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (339.40, 129.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 93, #reads = 1073718 mem = 536878181 peak = 1166786560 [M::process] read 685010 sequences (100000286 bp) in 3.634 CPU sec, 4.190 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 284426, 4, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 295, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (264.83, 101.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 297, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (314.66, 148.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1079) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 241, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976) [M::mem_pestat] mean and std.dev: (348.56, 232.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1279) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686126 reads in 240.819 CPU sec, 7.844 real sec [M::process] read 684352 sequences (100000024 bp) in 2.529 CPU sec, 2.534 real sec... [M::mem_process_seqs] Processed 685616 reads in 164.612 CPU sec, 5.353 real sec sorting block 94, #reads = 1072094 mem = 536880891 peak = 1166786560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283746, 6, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 274, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (220.28, 63.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 300, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (317.65, 149.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1093) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (288, 483, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014) [M::mem_pestat] mean and std.dev: (399.27, 144.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1256) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684280 sequences (100000163 bp) in 3.702 CPU sec, 4.109 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 283897, 4, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (307, 319, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (273, 358) [M::mem_pestat] mean and std.dev: (315.89, 11.02) [M::mem_pestat] low and high boundaries for proper pairs: (256, 375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 304, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (321.54, 151.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 235, 769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1913) [M::mem_pestat] mean and std.dev: (339.36, 241.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2485) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685010 reads in 228.432 CPU sec, 7.366 real sec [M::process] read 683828 sequences (100000141 bp) in 2.664 CPU sec, 2.678 real sec... [M::mem_process_seqs] Processed 684352 reads in 158.236 CPU sec, 5.079 real sec sorting block 95, #reads = 1074455 mem = 536882825 peak = 1166786560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 283963, 0, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 212, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (259.23, 138.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1026) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 305, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (322.71, 153.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 252, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (262.72, 85.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683278 sequences (100000188 bp) in 3.689 CPU sec, 4.070 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 283757, 6, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 199, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (266.57, 180.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1005) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 306, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (322.55, 151.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1104) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 323, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1122) [M::mem_pestat] mean and std.dev: (335.67, 170.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1425) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684280 reads in 234.701 CPU sec, 7.573 real sec [M::process] read 683960 sequences (100000126 bp) in 2.484 CPU sec, 2.628 real sec... [M::mem_process_seqs] Processed 683828 reads in 160.711 CPU sec, 5.148 real sec sorting block 96, #reads = 1074808 mem = 536878837 peak = 1166786560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 282912, 5, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 242, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937) [M::mem_pestat] mean and std.dev: (334.91, 228.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1249) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 310, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (326.13, 152.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 270, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (269.13, 81.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 97, #reads = 1073915 mem = 536880137 peak = 1166786560 [M::process] read 683740 sequences (100000107 bp) in 3.613 CPU sec, 4.192 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283666, 6, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 319, 739) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1877) [M::mem_pestat] mean and std.dev: (422.59, 334.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 306, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (323.31, 152.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 204, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1104) [M::mem_pestat] mean and std.dev: (326.86, 298.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1523) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683278 reads in 231.358 CPU sec, 7.480 real sec [M::process] read 682646 sequences (100000056 bp) in 2.631 CPU sec, 2.672 real sec... [M::mem_process_seqs] Processed 683960 reads in 163.248 CPU sec, 5.286 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283245, 0, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 194, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (202.21, 86.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 307, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (324.13, 152.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 263, 692) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1704) [M::mem_pestat] mean and std.dev: (420.62, 361.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2210) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 98, #reads = 1072323 mem = 536878386 peak = 1166786560 [M::process] read 682790 sequences (100000134 bp) in 3.509 CPU sec, 4.073 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282812, 7, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 225, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (36, 401) [M::mem_pestat] mean and std.dev: (204.55, 54.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 313, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (330.35, 155.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 389, 1231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3257) [M::mem_pestat] mean and std.dev: (720.60, 737.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4270) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683740 reads in 224.187 CPU sec, 7.323 real sec [M::process] read 683194 sequences (100000069 bp) in 2.530 CPU sec, 2.704 real sec... [M::mem_process_seqs] Processed 682646 reads in 158.219 CPU sec, 5.133 real sec sorting block 99, #reads = 1074414 mem = 536884214 peak = 1166786560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282581, 3, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 225, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1228) [M::mem_pestat] mean and std.dev: (293.45, 159.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 313, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (331.56, 157.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 247, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (252.95, 108.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681768 sequences (100000004 bp) in 3.562 CPU sec, 3.974 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 283166, 13, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (247, 393, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (352.12, 138.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1031) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 308, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (328.63, 158.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (462, 470, 1344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3108) [M::mem_pestat] mean and std.dev: (714.33, 580.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3990) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 247, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (254.35, 107.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682790 reads in 220.928 CPU sec, 7.098 real sec [M::process] read 681582 sequences (100000215 bp) in 2.817 CPU sec, 2.854 real sec... [M::mem_process_seqs] Processed 683194 reads in 155.789 CPU sec, 4.977 real sec sorting block 100, #reads = 1075074 mem = 536880465 peak = 1166786560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282374, 2, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048) [M::mem_pestat] mean and std.dev: (311.15, 184.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1343) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 319, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (337.84, 160.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1168) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (247, 254, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (163, 373) [M::mem_pestat] mean and std.dev: (259.33, 33.88) [M::mem_pestat] low and high boundaries for proper pairs: (121, 415) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681712 sequences (100000173 bp) in 3.414 CPU sec, 4.144 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281293, 4, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (240, 275, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 417) [M::mem_pestat] mean and std.dev: (264.92, 54.54) [M::mem_pestat] low and high boundaries for proper pairs: (47, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 321, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (339.98, 162.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1177) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 297, 560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1370) [M::mem_pestat] mean and std.dev: (325.08, 198.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1775) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681768 reads in 220.569 CPU sec, 7.162 real sec sorting block 101, #reads = 1076366 mem = 536877582 peak = 1166786560 [M::mem_process_seqs] Processed 681582 reads in 156.810 CPU sec, 5.001 real sec [M::process] read 681584 sequences (100000248 bp) in 2.950 CPU sec, 3.058 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 282190, 5, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 292, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (326.80, 164.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 984) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 323, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947) [M::mem_pestat] mean and std.dev: (341.80, 163.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1193) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 272, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021) [M::mem_pestat] mean and std.dev: (270.70, 152.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1304) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 102, #reads = 1074781 mem = 536881987 peak = 1166786560 [M::process] read 681006 sequences (100000211 bp) in 3.407 CPU sec, 3.934 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 282547, 2, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 262, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (240.17, 96.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 324, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940) [M::mem_pestat] mean and std.dev: (342.39, 163.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1183) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 248, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957) [M::mem_pestat] mean and std.dev: (252.90, 110.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1219) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681712 reads in 216.837 CPU sec, 7.055 real sec [M::mem_process_seqs] Processed 681584 reads in 153.856 CPU sec, 4.952 real sec [M::process] read 680788 sequences (100000110 bp) in 2.677 CPU sec, 2.799 real sec... sorting block 103, #reads = 1075893 mem = 536883468 peak = 1166786560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 281925, 3, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 330, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (257.29, 126.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 990) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 325, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (342.75, 162.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1173) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 283, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1157) [M::mem_pestat] mean and std.dev: (325.63, 221.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1489) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680622 sequences (100000297 bp) in 3.369 CPU sec, 3.797 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 281830, 6, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 267, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1094) [M::mem_pestat] mean and std.dev: (296.52, 140.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1397) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 327, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945) [M::mem_pestat] mean and std.dev: (344.74, 163.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1189) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 266, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (271.24, 123.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681006 reads in 223.438 CPU sec, 7.274 real sec [M::process] read 682916 sequences (100000092 bp) in 2.390 CPU sec, 2.436 real sec... [M::mem_process_seqs] Processed 680788 reads in 156.975 CPU sec, 5.117 real sec sorting block 104, #reads = 1074760 mem = 536880696 peak = 1166786560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 281918, 7, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 307, 535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1203) [M::mem_pestat] mean and std.dev: (336.64, 173.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 327, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940) [M::mem_pestat] mean and std.dev: (345.31, 163.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1182) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 314, 575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1265) [M::mem_pestat] mean and std.dev: (287.71, 169.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1610) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686872 sequences (100000073 bp) in 3.250 CPU sec, 3.712 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282201, 4, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 307, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1190) [M::mem_pestat] mean and std.dev: (290.11, 219.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 314, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921) [M::mem_pestat] mean and std.dev: (333.49, 160.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 217, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (263.71, 148.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685806 sequences (100000154 bp) in 2.501 CPU sec, 2.825 real sec... [M::mem_process_seqs] Processed 680622 reads in 245.449 CPU sec, 7.929 real sec sorting block 105, #reads = 1072390 mem = 536876814 peak = 1166786560 [M::mem_process_seqs] Processed 682916 reads in 181.330 CPU sec, 5.851 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 284477, 3, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 247, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (290.56, 183.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 289, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (312.26, 153.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1086) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (141, 245, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849) [M::mem_pestat] mean and std.dev: (253.33, 117.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1085) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684964 sequences (100000228 bp) in 3.266 CPU sec, 3.760 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 284381, 7, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 254, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (252.48, 82.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 296, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (317.26, 153.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 210, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (209.68, 77.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684516 sequences (100000023 bp) in 2.362 CPU sec, 2.422 real sec... [M::mem_process_seqs] Processed 686872 reads in 222.815 CPU sec, 7.170 real sec sorting block 106, #reads = 1065903 mem = 536879220 peak = 1166786560 [M::mem_process_seqs] Processed 685806 reads in 151.814 CPU sec, 4.884 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 283972, 0, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 277, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (273.88, 135.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 299, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (318.61, 152.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1092) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 269, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (42, 492) [M::mem_pestat] mean and std.dev: (255.52, 66.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 107, #reads = 1077910 mem = 536877680 peak = 1170579456 [M::process] read 683458 sequences (100000108 bp) in 3.606 CPU sec, 3.899 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 283883, 13, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (244, 379, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1099) [M::mem_pestat] mean and std.dev: (354.83, 154.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1384) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 303, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (322.11, 154.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 908, 2784) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8280) [M::mem_pestat] mean and std.dev: (1239.54, 1530.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11028) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 154, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (190.67, 65.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684964 reads in 210.904 CPU sec, 6.810 real sec [M::process] read 683564 sequences (100000246 bp) in 2.582 CPU sec, 2.623 real sec... [M::mem_process_seqs] Processed 684516 reads in 143.873 CPU sec, 4.657 real sec sorting block 108, #reads = 1078938 mem = 536879020 peak = 1170579456 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282585, 4, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 187, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (180.45, 46.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 307, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (324.87, 154.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 311, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (293.00, 94.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683326 sequences (100000253 bp) in 3.515 CPU sec, 4.075 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282751, 5, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 220, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (225.63, 134.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 310, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (328.33, 156.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 353, 611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1395) [M::mem_pestat] mean and std.dev: (316.13, 198.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1787) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683458 reads in 207.490 CPU sec, 6.753 real sec [M::mem_process_seqs] Processed 683564 reads in 144.416 CPU sec, 4.667 real sec [M::process] read 682924 sequences (100000285 bp) in 2.855 CPU sec, 3.095 real sec... sorting block 109, #reads = 1078093 mem = 536883402 peak = 1170579456 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 283315, 2, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 242, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (271.65, 117.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 311, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (330.35, 158.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 242, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840) [M::mem_pestat] mean and std.dev: (246.07, 105.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1069) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682728 sequences (100000005 bp) in 3.342 CPU sec, 3.693 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 282942, 3, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 242, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1100) [M::mem_pestat] mean and std.dev: (224.24, 123.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 313, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (331.93, 158.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 320, 577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1251) [M::mem_pestat] mean and std.dev: (379.59, 233.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1588) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683326 reads in 206.269 CPU sec, 6.669 real sec [M::mem_process_seqs] Processed 682924 reads in 145.336 CPU sec, 4.657 real sec sorting block 110, #reads = 1078573 mem = 536878951 peak = 1170579456 [M::process] read 682834 sequences (100000068 bp) in 2.801 CPU sec, 2.873 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282815, 0, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 247, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (259.65, 119.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1004) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 315, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (332.60, 158.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 254, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (241.08, 81.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683054 sequences (100000249 bp) in 3.381 CPU sec, 3.824 real sec... sorting block 111, #reads = 1078205 mem = 536880115 peak = 1181110272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282979, 12, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 257, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (256.42, 119.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 313, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (331.55, 157.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (332, 796, 877) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1967) [M::mem_pestat] mean and std.dev: (596.20, 269.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2512) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 252, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (23, 418) [M::mem_pestat] mean and std.dev: (221.52, 61.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682728 reads in 208.640 CPU sec, 6.768 real sec [M::mem_process_seqs] Processed 682834 reads in 149.476 CPU sec, 4.793 real sec [M::process] read 682716 sequences (100000068 bp) in 2.669 CPU sec, 2.757 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282805, 9, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 420, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1156) [M::mem_pestat] mean and std.dev: (305.07, 158.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 312, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (329.51, 156.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 302, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952) [M::mem_pestat] mean and std.dev: (278.96, 136.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1219) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 112, #reads = 1079301 mem = 536880562 peak = 1181110272 [M::process] read 682174 sequences (100000002 bp) in 3.132 CPU sec, 3.502 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 282637, 6, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 221, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (196.68, 76.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 315, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (332.77, 158.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 270, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (265.76, 88.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683054 reads in 212.209 CPU sec, 6.862 real sec [M::process] read 681710 sequences (100000207 bp) in 2.663 CPU sec, 2.688 real sec... [M::mem_process_seqs] Processed 682716 reads in 148.971 CPU sec, 4.779 real sec sorting block 113, #reads = 1076642 mem = 536881203 peak = 1181110272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282530, 5, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 274, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (231.00, 77.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 317, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (334.04, 157.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1136) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 376, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923) [M::mem_pestat] mean and std.dev: (322.04, 186.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1169) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681984 sequences (100000072 bp) in 3.374 CPU sec, 3.661 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 282526, 3, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 303, 1609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4503) [M::mem_pestat] mean and std.dev: (602.79, 597.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5950) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 319, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (335.37, 157.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (277, 282, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (313.79, 98.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682174 reads in 212.419 CPU sec, 6.865 real sec [M::process] read 681754 sequences (100000276 bp) in 2.448 CPU sec, 2.523 real sec... [M::mem_process_seqs] Processed 681710 reads in 146.636 CPU sec, 4.686 real sec sorting block 114, #reads = 1076681 mem = 536881638 peak = 1181110272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 282896, 4, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 338, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (307.26, 116.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 319, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (337.28, 160.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 294, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1105) [M::mem_pestat] mean and std.dev: (361.05, 215.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1401) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681506 sequences (100000273 bp) in 3.207 CPU sec, 3.519 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282597, 3, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 173, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (186.07, 78.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 320, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (337.88, 160.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 317, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784) [M::mem_pestat] mean and std.dev: (250.23, 131.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681984 reads in 210.975 CPU sec, 6.805 real sec [M::process] read 681480 sequences (100000102 bp) in 2.505 CPU sec, 2.605 real sec... sorting block 115, #reads = 1076838 mem = 536878637 peak = 1181110272 [M::mem_process_seqs] Processed 681754 reads in 146.061 CPU sec, 4.682 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 282660, 8, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 217, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (236.10, 101.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 322, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (339.58, 161.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1166) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 233, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (270.07, 150.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1090) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 116, #reads = 1076984 mem = 536878991 peak = 1181110272 [M::process] read 681716 sequences (100000106 bp) in 3.460 CPU sec, 4.030 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 282636, 6, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 332, 570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1330) [M::mem_pestat] mean and std.dev: (363.72, 248.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 319, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (337.39, 159.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1150) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (131, 193, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (236.59, 163.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681506 reads in 216.002 CPU sec, 6.978 real sec [M::mem_process_seqs] Processed 681480 reads in 150.912 CPU sec, 4.834 real sec [M::process] read 681262 sequences (100000143 bp) in 2.830 CPU sec, 2.923 real sec... sorting block 117, #reads = 1076939 mem = 536878433 peak = 1181110272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 281961, 9, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 165, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (164.12, 45.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 321, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (339.80, 161.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1166) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 284, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848) [M::mem_pestat] mean and std.dev: (293.11, 109.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1064) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681574 sequences (100000266 bp) in 3.433 CPU sec, 3.833 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282181, 7, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 207, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (233.94, 98.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 319, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (337.99, 159.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 364, 591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1311) [M::mem_pestat] mean and std.dev: (417.75, 227.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1671) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681716 reads in 221.153 CPU sec, 7.150 real sec [M::process] read 681428 sequences (100000134 bp) in 2.463 CPU sec, 2.465 real sec... [M::mem_process_seqs] Processed 681262 reads in 152.525 CPU sec, 4.919 real sec sorting block 118, #reads = 1074514 mem = 536880573 peak = 1181110272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282470, 4, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 251, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034) [M::mem_pestat] mean and std.dev: (294.92, 207.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1321) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 320, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923) [M::mem_pestat] mean and std.dev: (338.31, 160.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 254, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1089) [M::mem_pestat] mean and std.dev: (294.15, 146.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1403) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681562 sequences (100000246 bp) in 3.766 CPU sec, 4.123 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 281929, 3, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 229, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (235.00, 113.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 321, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (339.25, 161.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1162) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 277, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (290.96, 120.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681574 reads in 197.417 CPU sec, 6.352 real sec [M::mem_process_seqs] Processed 681428 reads in 125.169 CPU sec, 3.958 real sec sorting block 119, #reads = 1074679 mem = 536888068 peak = 1181110272 [M::process] read 681836 sequences (100000005 bp) in 2.501 CPU sec, 2.578 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 282704, 4, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 202, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968) [M::mem_pestat] mean and std.dev: (253.67, 196.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1232) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 320, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (338.66, 161.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1165) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 305, 638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1590) [M::mem_pestat] mean and std.dev: (427.55, 355.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2066) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 120, #reads = 1074564 mem = 536878624 peak = 1181110272 [M::process] read 681660 sequences (100000121 bp) in 2.705 CPU sec, 2.781 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 282166, 9, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 226, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1063) [M::mem_pestat] mean and std.dev: (244.00, 141.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 319, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (338.26, 161.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1165) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 282, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (288.03, 143.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681562 reads in 171.671 CPU sec, 5.434 real sec [M::process] read 681292 sequences (100000058 bp) in 2.311 CPU sec, 2.314 real sec... [M::mem_process_seqs] Processed 681836 reads in 121.835 CPU sec, 3.841 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281806, 8, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 182, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (183.62, 101.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 319, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (338.18, 161.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (135, 225, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (217.25, 86.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 121, #reads = 1075572 mem = 536879436 peak = 1181110272 [M::process] read 681714 sequences (100000003 bp) in 3.442 CPU sec, 3.603 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 281987, 6, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 162, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (196.73, 86.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 322, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (340.77, 162.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1163) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 269, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810) [M::mem_pestat] mean and std.dev: (302.58, 135.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1006) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 691052 sequences (100000133 bp) in 2.501 CPU sec, 2.505 real sec... [M::mem_process_seqs] Processed 681660 reads in 221.697 CPU sec, 7.073 real sec [M::mem_process_seqs] Processed 681292 reads in 154.644 CPU sec, 4.919 real sec sorting block 122, #reads = 1072336 mem = 536877036 peak = 1181110272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 281430, 3, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 217, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (297.91, 200.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1100) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 319, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (338.51, 163.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1164) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 340, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956) [M::mem_pestat] mean and std.dev: (318.25, 145.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1209) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688854 sequences (100000025 bp) in 3.268 CPU sec, 3.423 real sec... [M::process] read 687380 sequences (100000214 bp) in 2.549 CPU sec, 2.556 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 285865, 2, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 229, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (218.81, 77.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 269, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (292.44, 142.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1014) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 333, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093) [M::mem_pestat] mean and std.dev: (314.15, 167.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1403) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681714 reads in 251.170 CPU sec, 8.004 real sec [M::mem_process_seqs] Processed 691052 reads in 172.652 CPU sec, 5.493 real sec sorting block 123, #reads = 1068036 mem = 536877264 peak = 1181110272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 284862, 3, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 214, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979) [M::mem_pestat] mean and std.dev: (320.46, 242.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1292) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 278, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (300.59, 145.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 266, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (278.00, 179.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1165) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686164 sequences (100000212 bp) in 3.474 CPU sec, 3.915 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 284338, 2, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 247, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042) [M::mem_pestat] mean and std.dev: (258.39, 144.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1334) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 287, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848) [M::mem_pestat] mean and std.dev: (307.41, 147.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1068) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 263, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (272.70, 103.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685568 sequences (100000267 bp) in 2.521 CPU sec, 2.551 real sec... [M::mem_process_seqs] Processed 688854 reads in 237.887 CPU sec, 7.638 real sec sorting block 124, #reads = 1070328 mem = 536878085 peak = 1181110272 [M::mem_process_seqs] Processed 687380 reads in 161.017 CPU sec, 5.147 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283740, 3, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 210, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (240.24, 146.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1092) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 292, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (309.80, 146.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 292, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (274.91, 99.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 125, #reads = 1073343 mem = 536880307 peak = 1181110272 [M::process] read 685626 sequences (100000051 bp) in 3.425 CPU sec, 3.582 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 284102, 6, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 227, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (218.29, 95.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 296, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (313.26, 147.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1074) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 254, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986) [M::mem_pestat] mean and std.dev: (319.57, 217.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1254) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684768 sequences (100000070 bp) in 2.548 CPU sec, 2.549 real sec... [M::mem_process_seqs] Processed 686164 reads in 230.561 CPU sec, 7.348 real sec [M::mem_process_seqs] Processed 685568 reads in 156.211 CPU sec, 4.963 real sec sorting block 126, #reads = 1073041 mem = 536880786 peak = 1181110272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 283811, 9, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 159, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (161.91, 69.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 298, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (316.97, 151.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 241, 575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1405) [M::mem_pestat] mean and std.dev: (387.70, 350.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1820) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684420 sequences (100000024 bp) in 3.446 CPU sec, 3.575 real sec... [M::process] read 683722 sequences (100000268 bp) in 2.550 CPU sec, 2.556 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 283387, 3, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 315, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966) [M::mem_pestat] mean and std.dev: (313.85, 174.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1236) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 301, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (319.73, 152.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (139, 175, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018) [M::mem_pestat] mean and std.dev: (173.92, 70.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1311) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685626 reads in 242.351 CPU sec, 7.710 real sec [M::mem_process_seqs] Processed 684768 reads in 158.928 CPU sec, 5.049 real sec sorting block 127, #reads = 1071300 mem = 536877648 peak = 1181110272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 283455, 10, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 167, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (155.95, 51.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 303, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (322.35, 153.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (551, 1033, 1033) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1997) [M::mem_pestat] mean and std.dev: (765.50, 314.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2479) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 266, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (230.09, 83.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684010 sequences (100000099 bp) in 3.460 CPU sec, 3.597 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 283068, 6, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 274, 582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1306) [M::mem_pestat] mean and std.dev: (294.84, 149.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 308, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (326.25, 154.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 213, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (244.07, 108.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683746 sequences (100000031 bp) in 2.525 CPU sec, 2.528 real sec... [M::mem_process_seqs] Processed 684420 reads in 220.955 CPU sec, 7.014 real sec sorting block 128, #reads = 1074026 mem = 536880691 peak = 1181110272 [M::mem_process_seqs] Processed 683722 reads in 154.526 CPU sec, 4.894 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 283093, 6, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 307, 551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1225) [M::mem_pestat] mean and std.dev: (334.17, 167.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 304, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (322.36, 152.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 237, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (304.22, 189.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682392 sequences (100000238 bp) in 2.906 CPU sec, 2.998 real sec... [M::process] read 681982 sequences (100000245 bp) in 2.288 CPU sec, 2.298 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283025, 8, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 239, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797) [M::mem_pestat] mean and std.dev: (246.93, 125.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1007) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 305, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (322.91, 151.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 269, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953) [M::mem_pestat] mean and std.dev: (318.93, 169.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1202) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684010 reads in 220.183 CPU sec, 7.000 real sec sorting block 129, #reads = 1074746 mem = 536879802 peak = 1181110272 [M::mem_process_seqs] Processed 683746 reads in 151.947 CPU sec, 4.834 real sec sorting block 130, #reads = 1071891 mem = 536881741 peak = 1181110272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282354, 4, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 224, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (275.30, 159.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 314, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (330.54, 154.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 265, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (277.52, 137.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682832 sequences (100000185 bp) in 3.084 CPU sec, 3.266 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282321, 10, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 311, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (320.75, 168.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 317, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (332.46, 154.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (239, 1305, 1894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5204) [M::mem_pestat] mean and std.dev: (1134.60, 841.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6859) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 358, 556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1286) [M::mem_pestat] mean and std.dev: (371.58, 210.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1651) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682510 sequences (100000023 bp) in 2.401 CPU sec, 2.403 real sec... [M::mem_process_seqs] Processed 682392 reads in 218.637 CPU sec, 6.967 real sec [M::mem_process_seqs] Processed 681982 reads in 153.242 CPU sec, 4.874 real sec sorting block 131, #reads = 1073300 mem = 536885699 peak = 1181110272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282334, 7, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 306, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035) [M::mem_pestat] mean and std.dev: (295.58, 166.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1327) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 310, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (326.69, 153.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 281, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (245.25, 75.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682332 sequences (100000212 bp) in 2.658 CPU sec, 2.732 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282717, 3, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (269, 371, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (353.84, 138.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 310, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (328.01, 153.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 277, 742) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1850) [M::mem_pestat] mean and std.dev: (346.77, 217.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2404) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682132 sequences (100000235 bp) in 2.340 CPU sec, 2.334 real sec... [M::mem_process_seqs] Processed 682832 reads in 178.904 CPU sec, 5.665 real sec [M::mem_process_seqs] Processed 682510 reads in 115.492 CPU sec, 3.646 real sec sorting block 132, #reads = 1072708 mem = 536880877 peak = 1181110272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282708, 1, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 249, 613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1601) [M::mem_pestat] mean and std.dev: (383.95, 370.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2095) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 312, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (329.36, 154.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 302, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (295.58, 169.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682232 sequences (100000149 bp) in 3.182 CPU sec, 3.252 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 281962, 6, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 224, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841) [M::mem_pestat] mean and std.dev: (247.81, 145.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 314, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (331.54, 155.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 249, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (263.62, 129.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682332 reads in 174.014 CPU sec, 5.495 real sec sorting block 133, #reads = 1073997 mem = 536880154 peak = 1181110272 [M::process] read 681918 sequences (100000133 bp) in 2.466 CPU sec, 2.466 real sec... [M::mem_process_seqs] Processed 682132 reads in 118.106 CPU sec, 3.716 real sec sorting block 134, #reads = 1075249 mem = 536881241 peak = 1181110272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282754, 5, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 268, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (248.28, 122.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 314, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (331.29, 155.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 238, 612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1496) [M::mem_pestat] mean and std.dev: (321.02, 244.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1938) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681966 sequences (100000073 bp) in 2.774 CPU sec, 2.844 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 281974, 5, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 226, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (217.60, 89.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 315, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (332.92, 156.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 268, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (272.40, 111.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681756 sequences (100000167 bp) in 2.280 CPU sec, 2.280 real sec... [M::mem_process_seqs] Processed 682232 reads in 175.693 CPU sec, 5.555 real sec [M::mem_process_seqs] Processed 681918 reads in 119.384 CPU sec, 3.772 real sec sorting block 135, #reads = 1074928 mem = 536879660 peak = 1181110272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282186, 2, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 289, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (255.16, 111.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 316, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (334.56, 158.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1151) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 224, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (230.00, 101.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681810 sequences (100000136 bp) in 3.044 CPU sec, 3.132 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282438, 10, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 178, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (187.24, 82.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 317, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (335.20, 158.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1151) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1329, 1329, 2376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4470) [M::mem_pestat] mean and std.dev: (1641.10, 773.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5517) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (248, 369, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (345.34, 133.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681094 sequences (100000074 bp) in 2.310 CPU sec, 2.302 real sec... [M::mem_process_seqs] Processed 681966 reads in 176.120 CPU sec, 5.549 real sec [M::mem_process_seqs] Processed 681756 reads in 196.904 CPU sec, 6.227 real sec sorting block 136, #reads = 1074858 mem = 536880379 peak = 1181110272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282293, 2, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 279, 1263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3351) [M::mem_pestat] mean and std.dev: (789.39, 974.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 317, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (336.16, 159.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 217, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (241.17, 83.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680414 sequences (100000039 bp) in 3.009 CPU sec, 3.078 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282261, 6, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 278, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (287.93, 135.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1171) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 324, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932) [M::mem_pestat] mean and std.dev: (341.88, 162.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 232, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (215.43, 78.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 993) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681810 reads in 178.848 CPU sec, 5.651 real sec [M::process] read 680910 sequences (100000042 bp) in 2.259 CPU sec, 2.264 real sec... sorting block 137, #reads = 1074752 mem = 536879457 peak = 1181110272 [M::mem_process_seqs] Processed 681094 reads in 122.698 CPU sec, 3.867 real sec [M::process] read 257248 sequences (37729838 bp) in 1.436 CPU sec, 1.474 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282093, 5, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 268, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076) [M::mem_pestat] mean and std.dev: (299.52, 164.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 328, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (345.61, 163.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1179) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (272, 376, 594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1238) [M::mem_pestat] mean and std.dev: (362.94, 186.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1560) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 282175, 5, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (187.09, 56.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 322, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (340.13, 160.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 309, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (298.21, 120.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 106495, 1, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 316, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (334.59, 158.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 566, 710) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1718) [M::mem_pestat] mean and std.dev: (388.12, 201.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2222) [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680414 reads in 200.545 CPU sec, 6.460 real sec sorting block 138, #reads = 1074440 mem = 536880963 peak = 1181110272 [M::mem_process_seqs] Processed 680910 reads in 146.562 CPU sec, 4.737 real sec [M::mem_process_seqs] Processed 257248 reads in 156.364 CPU sec, 5.070 real sec sorting block 139, #reads = 1074348 mem = 536883185 peak = 1242484736 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000606a6 features: 1f8bfbff fffa320b extended: f1bf07ab 00407f5a amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8375C CPU @ 2.90GHz threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 150347744 overall: 69993734144 mem 27255.938 user 657.829 sys 924.818 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_5Q-T1-TDNA-1_A23MHWWLT4_1.L007\tPU:L007\tSM:1136_5Q-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_5Q-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1136_5Q-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 1136_5Q-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 924.856 sec; CPU: 27913.771 sec sorting block 140, #reads = 488200 mem = 244843126 peak = 1242484736 uniq header count 1 merging 141 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000606a6 features: 1f8bfbff fffa320b extended: f1bf07ab 00407f5a amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8375C CPU @ 2.90GHz threads: 31 max 128 algo: util-sort license: sentieon:util=1 output file size: 8368781269 output reads: 151234433 bam_mem_sort: 141 calls 175.090 user 4.886 sys 183.663 real bam_write: 141 calls 111.280 user 9.081 sys 228.106 real execute: 1 calls 46.822 user 1.295 sys 987.421 real merge_files: 1 calls 46.793 user 1.268 sys 61.895 real parse_chunk: 2248 calls 148.429 user 37.876 sys 231.727 real read_chunk: 6063 calls 83.977 user 40.926 sys 872.242 real sort_block: 1 calls 61.863 user 39.610 sys 844.454 real write_chunk: 3813 calls 46.276 user 0.937 sys 60.643 real overall: 3373256704 mem 2601.195 user 119.268 sys 987.443 real ls: cannot access '*.cram': No such file or directory