cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1047_JG-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1606.042 CPU sec, 56.433 real sec
[M::process] read 704364 sequences (100000058 bp) in 1.029 CPU sec, 1.032 real sec...
[M::process] read 701914 sequences (100000295 bp) in 1.287 CPU sec, 1.335 real sec...
[M::process] read 701230 sequences (100000046 bp) in 1.282 CPU sec, 1.307 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292923, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 212, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (235.22, 111.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700458 sequences (100000208 bp) in 1.238 CPU sec, 1.267 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291970, 6, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.75, 115.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 274, 680)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1706)
[M::mem_pestat] mean and std.dev: (376.92, 267.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2219)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 292041, 1, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 171, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (225.56, 129.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (244.13, 116.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 237, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (286.80, 148.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 704364 reads in 222.078 CPU sec, 7.175 real sec
[M::mem_process_seqs] Processed 701914 reads in 146.752 CPU sec, 4.852 real sec
[M::mem_process_seqs] Processed 701230 reads in 159.922 CPU sec, 5.318 real sec
sorting block 0, #reads = 1096601 mem = 536880102 peak = 586633216
[M::process] read 700118 sequences (100000003 bp) in 1.993 CPU sec, 2.143 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291620, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (246.30, 118.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699282 sequences (100000071 bp) in 1.387 CPU sec, 1.401 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291812, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (248.39, 120.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700458 reads in 133.199 CPU sec, 4.251 real sec
sorting block 1, #reads = 1094863 mem = 536882393 peak = 890736640
[M::mem_process_seqs] Processed 700118 reads in 82.594 CPU sec, 2.604 real sec
[M::process] read 698880 sequences (100000178 bp) in 1.383 CPU sec, 1.414 real sec...
sorting block 2, #reads = 1094813 mem = 536879017 peak = 914710528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291138, 5, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 283, 2299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6547)
[M::mem_pestat] mean and std.dev: (1025.70, 1019.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8671)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.25, 120.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698414 sequences (100000094 bp) in 1.912 CPU sec, 2.004 real sec...
[M::process] read 697988 sequences (100000179 bp) in 1.320 CPU sec, 1.327 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291108, 4, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 392, 5344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15690)
[M::mem_pestat] mean and std.dev: (1778.20, 2336.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 20863)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (251.88, 121.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699282 reads in 131.332 CPU sec, 4.188 real sec
[M::mem_process_seqs] Processed 698880 reads in 81.871 CPU sec, 2.606 real sec
sorting block 3, #reads = 1094956 mem = 536877911 peak = 914710528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290955, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (252.00, 121.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697948 sequences (100000098 bp) in 1.873 CPU sec, 1.970 real sec...
[M::process] read 697376 sequences (100000092 bp) in 1.330 CPU sec, 1.334 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290856, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.44, 121.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698414 reads in 131.727 CPU sec, 4.194 real sec
[M::mem_process_seqs] Processed 697988 reads in 81.774 CPU sec, 2.604 real sec
sorting block 4, #reads = 1094110 mem = 536879627 peak = 914710528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290515, 7, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (115, 237, 237)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481)
[M::mem_pestat] mean and std.dev: (164.12, 60.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 603)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.40, 121.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697064 sequences (100000187 bp) in 1.887 CPU sec, 1.987 real sec...
[M::process] read 697124 sequences (100000005 bp) in 1.390 CPU sec, 1.391 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290795, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.38, 119.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697948 reads in 134.495 CPU sec, 4.285 real sec
sorting block 5, #reads = 1092841 mem = 536879210 peak = 914710528
[M::mem_process_seqs] Processed 697376 reads in 83.610 CPU sec, 2.657 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 289961, 3, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (212, 318, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (286.00, 94.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.04, 118.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 6, #reads = 1092562 mem = 536877420 peak = 914710528
[M::process] read 696888 sequences (100000204 bp) in 2.232 CPU sec, 2.335 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290564, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (250.12, 117.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697028 sequences (100000085 bp) in 1.490 CPU sec, 1.499 real sec...
[M::mem_process_seqs] Processed 697064 reads in 136.165 CPU sec, 4.334 real sec
[M::mem_process_seqs] Processed 697124 reads in 85.150 CPU sec, 2.699 real sec
sorting block 7, #reads = 1092334 mem = 536879248 peak = 914710528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290558, 10, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (250.53, 117.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (462, 1013, 2060)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5256)
[M::mem_pestat] mean and std.dev: (1148.20, 652.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6854)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 224, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1171)
[M::mem_pestat] mean and std.dev: (339.64, 250.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1496)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696856 sequences (100000035 bp) in 2.039 CPU sec, 2.173 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290556, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (201, 213, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (235.67, 81.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (250.72, 117.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 696340 sequences (100000159 bp) in 1.460 CPU sec, 1.456 real sec...
[M::mem_process_seqs] Processed 696888 reads in 136.232 CPU sec, 4.347 real sec
[M::mem_process_seqs] Processed 697028 reads in 85.564 CPU sec, 2.715 real sec
sorting block 8, #reads = 1091896 mem = 536880701 peak = 914710528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290275, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (252.11, 118.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699900 sequences (100000002 bp) in 2.375 CPU sec, 2.472 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290373, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (254.81, 121.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702438 sequences (100000037 bp) in 1.478 CPU sec, 1.485 real sec...
[M::mem_process_seqs] Processed 696856 reads in 139.507 CPU sec, 4.439 real sec
[M::mem_process_seqs] Processed 696340 reads in 87.640 CPU sec, 2.778 real sec
sorting block 9, #reads = 1091392 mem = 536877274 peak = 914710528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291365, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (245.77, 116.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701460 sequences (100000048 bp) in 2.107 CPU sec, 2.210 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292447, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (240.76, 115.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699900 reads in 138.185 CPU sec, 4.412 real sec
[M::process] read 701082 sequences (100000238 bp) in 1.500 CPU sec, 1.491 real sec...
sorting block 10, #reads = 1088651 mem = 536880209 peak = 914710528
[M::mem_process_seqs] Processed 702438 reads in 85.689 CPU sec, 2.715 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292138, 3, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (243.88, 116.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 279, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (280.83, 87.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 782)
[M::mem_pestat] skip orientation RR
sorting block 11, #reads = 1093977 mem = 536878418 peak = 938901504
[M::process] read 700502 sequences (100000090 bp) in 2.491 CPU sec, 2.593 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292069, 2, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 221, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (246.21, 119.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 204, 282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 468)
[M::mem_pestat] mean and std.dev: (202.15, 47.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 561)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 701460 reads in 135.168 CPU sec, 4.314 real sec
[M::mem_process_seqs] Processed 701082 reads in 85.034 CPU sec, 2.689 real sec
[M::process] read 700134 sequences (100000243 bp) in 1.694 CPU sec, 1.717 real sec...
sorting block 12, #reads = 1096449 mem = 536878343 peak = 938901504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291437, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (247.80, 119.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699070 sequences (100000298 bp) in 2.119 CPU sec, 2.291 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291653, 10, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (249.05, 120.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 56, 60)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 164)
[M::mem_pestat] mean and std.dev: (36.00, 23.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 216)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 700502 reads in 135.890 CPU sec, 4.348 real sec
[M::process] read 698688 sequences (100000299 bp) in 1.647 CPU sec, 1.661 real sec...
sorting block 13, #reads = 1094228 mem = 536881699 peak = 938901504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291151, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.98, 121.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699070 reads in 74.058 CPU sec, 2.349 real sec
[M::process] read 698556 sequences (100000142 bp) in 1.869 CPU sec, 1.872 real sec...
[M::mem_process_seqs] Processed 700134 reads in 190.713 CPU sec, 6.061 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 291215, 4, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (244, 317, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (288.58, 92.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 784)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.07, 121.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 14, #reads = 1094516 mem = 536879194 peak = 938901504
[M::process] read 697716 sequences (100000235 bp) in 2.176 CPU sec, 2.325 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290727, 2, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 218, 234)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 382)
[M::mem_pestat] mean and std.dev: (192.58, 66.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 458)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (253.04, 122.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698688 reads in 146.635 CPU sec, 4.670 real sec
[M::mem_process_seqs] Processed 698556 reads in 85.867 CPU sec, 2.719 real sec
[M::process] read 697598 sequences (100000172 bp) in 1.955 CPU sec, 2.046 real sec...
sorting block 15, #reads = 1094310 mem = 536878060 peak = 938901504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291182, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (254.16, 122.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696876 sequences (100000148 bp) in 2.176 CPU sec, 2.309 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291026, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.26, 121.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697716 reads in 136.078 CPU sec, 4.342 real sec
[M::mem_process_seqs] Processed 697598 reads in 85.310 CPU sec, 2.696 real sec
[M::process] read 696646 sequences (100000167 bp) in 1.672 CPU sec, 1.714 real sec...
sorting block 16, #reads = 1094227 mem = 536878773 peak = 938901504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290628, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (252.85, 119.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697190 sequences (100000092 bp) in 2.161 CPU sec, 2.325 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290793, 8, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (251.82, 118.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696876 reads in 137.589 CPU sec, 4.399 real sec
sorting block 17, #reads = 1094095 mem = 536879061 peak = 938901504
[M::mem_process_seqs] Processed 696646 reads in 86.571 CPU sec, 2.738 real sec
[M::process] read 696854 sequences (100000184 bp) in 1.675 CPU sec, 1.711 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291089, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (250.87, 117.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 18, #reads = 1092351 mem = 536880884 peak = 954732544
[M::process] read 697268 sequences (100000167 bp) in 2.168 CPU sec, 2.296 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290343, 7, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 314, 2361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6711)
[M::mem_pestat] mean and std.dev: (1087.73, 1146.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8886)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.44, 118.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697190 reads in 131.434 CPU sec, 4.203 real sec
[M::mem_process_seqs] Processed 696854 reads in 83.791 CPU sec, 2.647 real sec
[M::process] read 697180 sequences (100000131 bp) in 1.795 CPU sec, 1.858 real sec...
sorting block 19, #reads = 1092874 mem = 536879717 peak = 954732544
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290753, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (251.08, 118.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697052 sequences (100000078 bp) in 2.001 CPU sec, 2.122 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290328, 10, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (280, 286, 2697)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7531)
[M::mem_pestat] mean and std.dev: (1042.09, 1069.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9948)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.27, 119.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (6, 101, 1604)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4800)
[M::mem_pestat] mean and std.dev: (783.80, 1049.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6398)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 697268 reads in 133.036 CPU sec, 4.256 real sec
[M::mem_process_seqs] Processed 697180 reads in 86.020 CPU sec, 2.719 real sec
[M::process] read 703612 sequences (100000124 bp) in 1.752 CPU sec, 1.815 real sec...
sorting block 20, #reads = 1091782 mem = 536879125 peak = 954732544
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290368, 3, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (277, 514, 1152)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2902)
[M::mem_pestat] mean and std.dev: (554.67, 371.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3777)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.22, 121.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 285, 874)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2214)
[M::mem_pestat] mean and std.dev: (388.75, 263.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2884)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 703548 sequences (100000023 bp) in 2.187 CPU sec, 2.337 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292510, 4, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (238.26, 114.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (283, 373, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (103, 553)
[M::mem_pestat] mean and std.dev: (314.45, 60.04)
[M::mem_pestat] low and high boundaries for proper pairs: (13, 643)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697052 reads in 135.858 CPU sec, 4.333 real sec
[M::mem_process_seqs] Processed 703612 reads in 85.131 CPU sec, 2.685 real sec
sorting block 21, #reads = 1090399 mem = 536888192 peak = 954732544
[M::process] read 702684 sequences (100000143 bp) in 1.682 CPU sec, 1.723 real sec...
sorting block 22, #reads = 1093185 mem = 536878120 peak = 957202432
[M::process] read 702168 sequences (100000007 bp) in 2.128 CPU sec, 2.276 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292683, 3, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (240.50, 115.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 375, 1270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3476)
[M::mem_pestat] mean and std.dev: (667.67, 841.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4579)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 702684 reads in 72.981 CPU sec, 2.297 real sec
[M::process] read 701038 sequences (100000081 bp) in 1.816 CPU sec, 1.899 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 293382, 6, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (253, 365, 774)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1816)
[M::mem_pestat] mean and std.dev: (440.36, 270.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2337)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (238.56, 114.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 292194, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.04, 116.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703548 reads in 203.147 CPU sec, 6.470 real sec
sorting block 23, #reads = 1096890 mem = 536876794 peak = 957202432
[M::process] read 700726 sequences (100000141 bp) in 2.037 CPU sec, 2.127 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291755, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (245.71, 119.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701038 reads in 74.127 CPU sec, 2.352 real sec
[M::process] read 699842 sequences (100000187 bp) in 1.792 CPU sec, 1.840 real sec...
sorting block 24, #reads = 1095423 mem = 536878077 peak = 957202432
[M::mem_process_seqs] Processed 702168 reads in 207.041 CPU sec, 6.565 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291542, 4, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (246.10, 119.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 415, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (257.10, 136.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RR
[M::process] read 699552 sequences (100000027 bp) in 2.207 CPU sec, 2.328 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291467, 5, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 320, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721)
[M::mem_pestat] mean and std.dev: (253.67, 93.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (248.35, 119.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 700726 reads in 143.525 CPU sec, 4.551 real sec
[M::mem_process_seqs] Processed 699842 reads in 83.659 CPU sec, 2.646 real sec
sorting block 25, #reads = 1096148 mem = 536881358 peak = 957202432
[M::process] read 699164 sequences (100000233 bp) in 2.030 CPU sec, 2.147 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291419, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (250.54, 122.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698842 sequences (100000187 bp) in 2.127 CPU sec, 2.238 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291830, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.48, 121.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699552 reads in 131.404 CPU sec, 4.191 real sec
sorting block 26, #reads = 1094834 mem = 536877203 peak = 957202432
[M::mem_process_seqs] Processed 699164 reads in 83.588 CPU sec, 2.642 real sec
[M::process] read 698706 sequences (100000141 bp) in 1.844 CPU sec, 1.933 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291145, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (251.11, 121.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 297, 553)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1131)
[M::mem_pestat] mean and std.dev: (303.38, 125.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1420)
[M::mem_pestat] skip orientation RR
sorting block 27, #reads = 1094374 mem = 536883428 peak = 970993664
[M::process] read 698802 sequences (100000248 bp) in 2.272 CPU sec, 2.429 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290939, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (246.63, 116.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698842 reads in 131.980 CPU sec, 4.212 real sec
[M::mem_process_seqs] Processed 698706 reads in 84.224 CPU sec, 2.665 real sec
[M::process] read 697420 sequences (100000010 bp) in 1.855 CPU sec, 1.935 real sec...
sorting block 28, #reads = 1093378 mem = 536878935 peak = 970993664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290854, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.35, 119.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697472 sequences (100000255 bp) in 2.170 CPU sec, 2.274 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291173, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (252.65, 120.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698802 reads in 131.455 CPU sec, 4.185 real sec
[M::mem_process_seqs] Processed 697420 reads in 83.668 CPU sec, 2.642 real sec
[M::process] read 697390 sequences (100000128 bp) in 1.643 CPU sec, 1.694 real sec...
sorting block 29, #reads = 1093544 mem = 536879322 peak = 976461824
[M::process] read 697764 sequences (100000050 bp) in 2.068 CPU sec, 2.209 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290573, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (252.80, 120.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697390 reads in 73.104 CPU sec, 2.312 real sec
sorting block 30, #reads = 1092027 mem = 536879051 peak = 976461824
[M::process] read 697080 sequences (100000018 bp) in 1.600 CPU sec, 1.624 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291315, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.69, 121.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290614, 3, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.51, 120.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 556, 1054)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2620)
[M::mem_pestat] mean and std.dev: (507.42, 327.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3403)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697472 reads in 205.242 CPU sec, 6.533 real sec
[M::mem_process_seqs] Processed 697764 reads in 98.536 CPU sec, 3.121 real sec
[M::process] read 702332 sequences (100000200 bp) in 2.012 CPU sec, 2.057 real sec...
sorting block 31, #reads = 1093205 mem = 536884023 peak = 985698304
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290478, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (254.80, 122.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703436 sequences (100000161 bp) in 2.297 CPU sec, 2.435 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292074, 8, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.32, 115.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (102, 305, 539)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1413)
[M::mem_pestat] mean and std.dev: (249.12, 145.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1850)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697080 reads in 133.681 CPU sec, 4.265 real sec
[M::mem_process_seqs] Processed 702332 reads in 85.408 CPU sec, 2.695 real sec
sorting block 32, #reads = 1091230 mem = 536877411 peak = 985698304
[M::process] read 702570 sequences (100000041 bp) in 1.788 CPU sec, 1.864 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292539, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (238.26, 114.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 33, #reads = 1092980 mem = 536884636 peak = 989573120
[M::process] read 702376 sequences (100000227 bp) in 2.235 CPU sec, 2.391 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292478, 2, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 620, 866)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2252)
[M::mem_pestat] mean and std.dev: (485.82, 317.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2945)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (240.90, 115.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 703436 reads in 129.612 CPU sec, 4.126 real sec
[M::mem_process_seqs] Processed 702570 reads in 82.620 CPU sec, 2.613 real sec
[M::process] read 701638 sequences (100000177 bp) in 1.674 CPU sec, 1.724 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 292292, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (240.43, 115.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 34, #reads = 1097134 mem = 536877611 peak = 989573120
[M::process] read 701156 sequences (100000192 bp) in 2.286 CPU sec, 2.417 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292122, 11, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (242.98, 116.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (73, 607, 1087)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3115)
[M::mem_pestat] mean and std.dev: (725.64, 671.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4129)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 702376 reads in 129.685 CPU sec, 4.138 real sec
[M::mem_process_seqs] Processed 701638 reads in 82.782 CPU sec, 2.616 real sec
[M::process] read 701142 sequences (100000215 bp) in 1.866 CPU sec, 1.951 real sec...
sorting block 35, #reads = 1096469 mem = 536882104 peak = 989573120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291801, 1, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (256, 354, 989)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2455)
[M::mem_pestat] mean and std.dev: (587.80, 480.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3188)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (244.63, 118.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 700058 sequences (100000201 bp) in 2.175 CPU sec, 2.293 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292195, 6, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (245.99, 119.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (129, 244, 754)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2004)
[M::mem_pestat] mean and std.dev: (252.18, 171.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2629)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 701156 reads in 129.695 CPU sec, 4.135 real sec
[M::process] read 699808 sequences (100000187 bp) in 1.835 CPU sec, 1.909 real sec...
sorting block 36, #reads = 1096583 mem = 536877619 peak = 989573120
[M::process] read 699630 sequences (100000135 bp) in 1.337 CPU sec, 1.341 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291558, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (248.88, 121.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699808 reads in 67.152 CPU sec, 2.186 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291911, 7, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (247.81, 119.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 439, 928)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2360)
[M::mem_pestat] mean and std.dev: (478.15, 300.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3076)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700058 reads in 139.074 CPU sec, 4.887 real sec
sorting block 37, #reads = 1094807 mem = 536879530 peak = 989573120
[M::mem_process_seqs] Processed 701142 reads in 275.295 CPU sec, 9.194 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291570, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (250.23, 121.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698820 sequences (100000207 bp) in 2.558 CPU sec, 2.728 real sec...
[M::mem_process_seqs] Processed 699630 reads in 84.107 CPU sec, 2.730 real sec
sorting block 38, #reads = 1095736 mem = 536881058 peak = 989573120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291402, 7, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.67, 121.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 274, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (300.07, 169.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1098)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698820 reads in 73.125 CPU sec, 2.411 real sec
[M::process] read 698120 sequences (100000218 bp) in 2.314 CPU sec, 2.508 real sec...
sorting block 39, #reads = 1095151 mem = 536877330 peak = 989573120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290952, 8, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (252.91, 122.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698120 reads in 74.420 CPU sec, 2.434 real sec
[M::process] read 697194 sequences (100000215 bp) in 2.340 CPU sec, 2.524 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 290923, 5, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (236, 485, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1085)
[M::mem_pestat] mean and std.dev: (430.38, 233.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1368)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (255.22, 122.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 852)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (517, 859, 864)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1558)
[M::mem_pestat] mean and std.dev: (758.00, 322.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2046)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 40, #reads = 1093522 mem = 536879401 peak = 989573120
[M::mem_process_seqs] Processed 697194 reads in 74.430 CPU sec, 2.411 real sec
[M::process] read 696694 sequences (100000150 bp) in 2.576 CPU sec, 2.789 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290493, 14, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (255.19, 121.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 221, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1367)
[M::mem_pestat] mean and std.dev: (194.31, 209.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1820)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 696574 sequences (100000294 bp) in 2.206 CPU sec, 2.332 real sec...
[M::mem_process_seqs] Processed 696694 reads in 74.583 CPU sec, 2.411 real sec
sorting block 41, #reads = 1092977 mem = 536879142 peak = 989573120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 289866, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (255.64, 121.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 849)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696480 sequences (100000282 bp) in 2.322 CPU sec, 2.479 real sec...
[M::mem_process_seqs] Processed 696574 reads in 74.852 CPU sec, 2.421 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290070, 9, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 159, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (197.22, 111.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.58, 120.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 187, 590)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1396)
[M::mem_pestat] mean and std.dev: (254.10, 142.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1799)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 42, #reads = 1092312 mem = 536879404 peak = 989573120
[M::mem_process_seqs] Processed 696480 reads in 75.646 CPU sec, 2.446 real sec
[M::process] read 696120 sequences (100000132 bp) in 2.343 CPU sec, 2.484 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290108, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.61, 120.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696120 reads in 76.586 CPU sec, 2.480 real sec
[M::process] read 700320 sequences (100000084 bp) in 2.705 CPU sec, 2.889 real sec...
sorting block 43, #reads = 1089296 mem = 536879008 peak = 989573120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291483, 2, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 272, 1078)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2926)
[M::mem_pestat] mean and std.dev: (498.83, 428.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3850)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (244.47, 116.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 706650 sequences (100000148 bp) in 2.168 CPU sec, 2.285 real sec...
[M::mem_process_seqs] Processed 700320 reads in 80.753 CPU sec, 2.599 real sec
[M::process] read 706278 sequences (100000299 bp) in 2.121 CPU sec, 2.235 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 293910, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 205, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (228.91, 109.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 739)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703994 sequences (100000028 bp) in 1.310 CPU sec, 1.318 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 293420, 0, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 207, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595)
[M::mem_pestat] mean and std.dev: (230.46, 110.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 744)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 291, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607)
[M::mem_pestat] mean and std.dev: (227.14, 87.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 760)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 706650 reads in 140.099 CPU sec, 4.404 real sec
sorting block 44, #reads = 1088743 mem = 536879057 peak = 989573120
[M::process] read 703012 sequences (100000136 bp) in 1.342 CPU sec, 1.345 real sec...
[M::mem_process_seqs] Processed 706278 reads in 88.724 CPU sec, 2.791 real sec
sorting block 45, #reads = 1095920 mem = 536878964 peak = 1085161472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292847, 7, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (236.16, 113.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701922 sequences (100000090 bp) in 2.125 CPU sec, 2.284 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 293085, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (239.73, 116.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 210, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (200.56, 57.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 727)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 703994 reads in 136.555 CPU sec, 4.344 real sec
[M::process] read 701630 sequences (100000109 bp) in 1.465 CPU sec, 1.471 real sec...
[M::process] read 700954 sequences (100000027 bp) in 1.543 CPU sec, 1.545 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 292476, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (243.22, 118.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701922 reads in 77.317 CPU sec, 2.635 real sec
sorting block 46, #reads = 1095001 mem = 536878118 peak = 1085161472
[M::mem_process_seqs] Processed 703012 reads in 188.225 CPU sec, 6.161 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292117, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (244.59, 119.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 47, #reads = 1095048 mem = 536879409 peak = 1085161472
[M::process] read 700958 sequences (100000075 bp) in 1.975 CPU sec, 2.079 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292134, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (247.53, 121.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701630 reads in 148.747 CPU sec, 4.725 real sec
[M::process] read 699086 sequences (100000290 bp) in 1.576 CPU sec, 1.601 real sec...
[M::mem_process_seqs] Processed 700954 reads in 87.323 CPU sec, 2.751 real sec
sorting block 48, #reads = 1093603 mem = 536880252 peak = 1085161472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291887, 5, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (247.30, 121.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (280, 280, 839)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1957)
[M::mem_pestat] mean and std.dev: (395.78, 246.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2516)
[M::mem_pestat] skip orientation RR
[M::process] read 698584 sequences (100000026 bp) in 2.305 CPU sec, 2.406 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291172, 3, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (196, 739, 3140)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9028)
[M::mem_pestat] mean and std.dev: (1437.60, 1440.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11972)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.48, 120.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 209, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (205.36, 62.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 697978 sequences (100000025 bp) in 1.460 CPU sec, 1.462 real sec...
[M::mem_process_seqs] Processed 700958 reads in 138.076 CPU sec, 4.386 real sec
[M::mem_process_seqs] Processed 699086 reads in 88.111 CPU sec, 2.785 real sec
sorting block 49, #reads = 1092840 mem = 536879675 peak = 1085161472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291489, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 325, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (285.67, 151.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (250.35, 121.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698522 sequences (100000212 bp) in 2.060 CPU sec, 2.173 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290761, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.50, 120.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (382, 418, 965)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2131)
[M::mem_pestat] mean and std.dev: (489.70, 303.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2714)
[M::mem_pestat] skip orientation RR
[M::process] read 698704 sequences (100000038 bp) in 1.450 CPU sec, 1.449 real sec...
[M::mem_process_seqs] Processed 698584 reads in 138.617 CPU sec, 4.407 real sec
sorting block 50, #reads = 1091310 mem = 536878084 peak = 1085161472
[M::mem_process_seqs] Processed 697978 reads in 86.925 CPU sec, 2.751 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 290939, 1, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (250, 950, 950)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2350)
[M::mem_pestat] mean and std.dev: (797.79, 617.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3267)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.32, 120.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 51, #reads = 1091476 mem = 536879027 peak = 1085161472
[M::process] read 698620 sequences (100000029 bp) in 2.408 CPU sec, 2.511 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291239, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.06, 118.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697900 sequences (100000251 bp) in 1.470 CPU sec, 1.468 real sec...
[M::mem_process_seqs] Processed 698522 reads in 138.990 CPU sec, 4.409 real sec
[M::mem_process_seqs] Processed 698704 reads in 88.684 CPU sec, 2.801 real sec
sorting block 52, #reads = 1090441 mem = 536878632 peak = 1085161472
[M::process] read 698242 sequences (100000184 bp) in 2.171 CPU sec, 2.315 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290374, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.83, 119.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 707260 sequences (100000201 bp) in 1.540 CPU sec, 1.542 real sec...
[M::mem_process_seqs] Processed 697900 reads in 81.809 CPU sec, 2.579 real sec
sorting block 53, #reads = 1089543 mem = 536876947 peak = 1085161472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290900, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.54, 119.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290856, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (249.18, 119.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698620 reads in 216.617 CPU sec, 6.885 real sec
[M::process] read 707420 sequences (100000024 bp) in 1.879 CPU sec, 1.909 real sec...
[M::mem_process_seqs] Processed 698242 reads in 103.939 CPU sec, 3.303 real sec
sorting block 54, #reads = 1089853 mem = 536877758 peak = 1085161472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 293717, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 205, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (228.05, 107.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 735)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 706860 sequences (100000170 bp) in 2.543 CPU sec, 2.680 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 294166, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 204, 292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584)
[M::mem_pestat] mean and std.dev: (227.33, 107.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 705240 sequences (100000181 bp) in 1.490 CPU sec, 1.493 real sec...
[M::mem_process_seqs] Processed 707260 reads in 153.218 CPU sec, 4.871 real sec
[M::mem_process_seqs] Processed 707420 reads in 96.945 CPU sec, 3.073 real sec
sorting block 55, #reads = 1088416 mem = 536880873 peak = 1085161472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 293866, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 205, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (229.39, 109.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 743)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704382 sequences (100000159 bp) in 2.807 CPU sec, 2.977 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 293184, 3, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 158, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693)
[M::mem_pestat] mean and std.dev: (210.44, 112.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 209, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605)
[M::mem_pestat] mean and std.dev: (233.34, 111.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 757)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 706860 reads in 156.794 CPU sec, 4.971 real sec
[M::process] read 702810 sequences (100000239 bp) in 1.710 CPU sec, 1.709 real sec...
[M::mem_process_seqs] Processed 705240 reads in 98.693 CPU sec, 3.116 real sec
sorting block 56, #reads = 1091256 mem = 536877759 peak = 1085161472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 293156, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (236.32, 114.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702176 sequences (100000181 bp) in 2.856 CPU sec, 3.007 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292509, 8, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (239.76, 115.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 704382 reads in 149.951 CPU sec, 4.764 real sec
sorting block 57, #reads = 1089734 mem = 536880272 peak = 1085161472
[M::mem_process_seqs] Processed 702810 reads in 93.613 CPU sec, 2.951 real sec
[M::process] read 701972 sequences (100000154 bp) in 1.817 CPU sec, 1.853 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292512, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.65, 116.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 58, #reads = 1090869 mem = 536880085 peak = 1085161472
[M::process] read 701264 sequences (100000078 bp) in 2.717 CPU sec, 2.892 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291870, 5, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (217, 345, 974)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2488)
[M::mem_pestat] mean and std.dev: (619.00, 539.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3245)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.74, 117.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 702176 reads in 146.629 CPU sec, 4.673 real sec
[M::mem_process_seqs] Processed 701972 reads in 92.902 CPU sec, 2.931 real sec
[M::process] read 700280 sequences (100000063 bp) in 1.966 CPU sec, 2.041 real sec...
sorting block 59, #reads = 1091772 mem = 536878690 peak = 1085161472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292154, 10, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (244.14, 118.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 773, 3534)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10212)
[M::mem_pestat] mean and std.dev: (1439.40, 1533.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 13551)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 699472 sequences (100000191 bp) in 2.570 CPU sec, 2.713 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291484, 9, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (246.32, 118.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701264 reads in 145.113 CPU sec, 4.625 real sec
[M::mem_process_seqs] Processed 700280 reads in 92.182 CPU sec, 2.908 real sec
[M::process] read 698866 sequences (100000258 bp) in 1.976 CPU sec, 2.057 real sec...
sorting block 60, #reads = 1092124 mem = 536877779 peak = 1085161472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290743, 4, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 462, 1002)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2710)
[M::mem_pestat] mean and std.dev: (515.08, 364.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3564)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (248.36, 120.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698620 sequences (100000298 bp) in 2.306 CPU sec, 2.446 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291081, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (249.04, 120.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699472 reads in 140.083 CPU sec, 4.475 real sec
[M::mem_process_seqs] Processed 698866 reads in 88.888 CPU sec, 2.806 real sec
sorting block 61, #reads = 1091335 mem = 536878972 peak = 1085161472
[M::process] read 698900 sequences (100000137 bp) in 2.263 CPU sec, 2.392 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290874, 9, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 829, 3418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9916)
[M::mem_pestat] mean and std.dev: (1645.92, 1824.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 13165)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.83, 120.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 62, #reads = 1090812 mem = 536879462 peak = 1085161472
[M::process] read 698712 sequences (100000202 bp) in 2.354 CPU sec, 2.480 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291315, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.63, 120.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698620 reads in 139.569 CPU sec, 4.458 real sec
[M::mem_process_seqs] Processed 698900 reads in 88.923 CPU sec, 2.811 real sec
[M::process] read 698494 sequences (100000290 bp) in 2.050 CPU sec, 2.140 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291003, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.99, 120.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 63, #reads = 1089932 mem = 536881934 peak = 1085161472
[M::process] read 706592 sequences (100000281 bp) in 2.329 CPU sec, 2.485 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290562, 5, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 336, 585)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1491)
[M::mem_pestat] mean and std.dev: (373.56, 274.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1944)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (249.05, 120.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 357, 907)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2185)
[M::mem_pestat] mean and std.dev: (321.80, 213.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2824)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698712 reads in 148.652 CPU sec, 4.730 real sec
[M::process] read 706796 sequences (100000116 bp) in 1.673 CPU sec, 1.704 real sec...
[M::mem_process_seqs] Processed 698494 reads in 99.167 CPU sec, 3.128 real sec
sorting block 64, #reads = 1090345 mem = 536880622 peak = 1085161472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 293658, 6, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 154, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737)
[M::mem_pestat] mean and std.dev: (206.20, 148.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 206, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592)
[M::mem_pestat] mean and std.dev: (229.38, 108.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 740)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 704940 sequences (100000018 bp) in 2.519 CPU sec, 2.647 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 294119, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 205, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (228.98, 108.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 739)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 706592 reads in 143.519 CPU sec, 4.561 real sec
[M::process] read 703800 sequences (100000031 bp) in 1.499 CPU sec, 1.498 real sec...
[M::mem_process_seqs] Processed 706796 reads in 89.574 CPU sec, 2.833 real sec
sorting block 65, #reads = 1084209 mem = 536881436 peak = 1085161472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 293370, 2, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (120, 221, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627)
[M::mem_pestat] mean and std.dev: (171.12, 64.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 209, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605)
[M::mem_pestat] mean and std.dev: (233.01, 111.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 757)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 702652 sequences (100000201 bp) in 2.511 CPU sec, 2.631 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292806, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (236.13, 113.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 704940 reads in 137.226 CPU sec, 4.361 real sec
sorting block 66, #reads = 1096415 mem = 536876374 peak = 1085161472
[M::process] read 702072 sequences (100000217 bp) in 1.599 CPU sec, 1.622 real sec...
[M::mem_process_seqs] Processed 703800 reads in 86.133 CPU sec, 2.715 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292614, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (239.56, 115.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 67, #reads = 1095412 mem = 536885455 peak = 1085161472
[M::process] read 701632 sequences (100000175 bp) in 2.448 CPU sec, 2.563 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292497, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 215, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627)
[M::mem_pestat] mean and std.dev: (240.15, 114.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702652 reads in 132.565 CPU sec, 4.221 real sec
[M::mem_process_seqs] Processed 702072 reads in 84.234 CPU sec, 2.655 real sec
[M::process] read 701516 sequences (100000065 bp) in 1.866 CPU sec, 1.921 real sec...
sorting block 68, #reads = 1095437 mem = 536879598 peak = 1085161472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292333, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.77, 115.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701270 sequences (100000123 bp) in 2.446 CPU sec, 2.583 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291876, 10, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (242.53, 116.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (414, 1041, 2578)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6906)
[M::mem_pestat] mean and std.dev: (891.38, 757.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9070)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 221, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (261.92, 111.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 701632 reads in 135.237 CPU sec, 4.307 real sec
[M::mem_process_seqs] Processed 701516 reads in 86.605 CPU sec, 2.737 real sec
[M::process] read 701474 sequences (100000108 bp) in 1.958 CPU sec, 2.035 real sec...
sorting block 69, #reads = 1094562 mem = 536878736 peak = 1085161472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291901, 8, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 253, 1160)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3128)
[M::mem_pestat] mean and std.dev: (578.82, 531.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4112)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (242.81, 116.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 701788 sequences (100000185 bp) in 2.392 CPU sec, 2.520 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292302, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 215, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627)
[M::mem_pestat] mean and std.dev: (240.43, 114.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701270 reads in 137.691 CPU sec, 4.387 real sec
[M::mem_process_seqs] Processed 701474 reads in 87.218 CPU sec, 2.753 real sec
sorting block 70, #reads = 1093554 mem = 536880223 peak = 1085161472
[M::process] read 701430 sequences (100000032 bp) in 2.001 CPU sec, 2.100 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 292067, 7, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 215, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (239.65, 114.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 781)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 279, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (272.50, 138.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] skip orientation RR
sorting block 71, #reads = 1093100 mem = 536878676 peak = 1085161472
[M::process] read 700528 sequences (100000055 bp) in 2.447 CPU sec, 2.598 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292550, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (242.20, 116.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701788 reads in 138.777 CPU sec, 4.428 real sec
[M::mem_process_seqs] Processed 701430 reads in 88.372 CPU sec, 2.797 real sec
[M::process] read 699540 sequences (100000243 bp) in 2.042 CPU sec, 2.137 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291999, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 219, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (245.08, 118.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 72, #reads = 1093644 mem = 536877964 peak = 1085161472
[M::process] read 699568 sequences (100000267 bp) in 2.362 CPU sec, 2.524 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291674, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (247.15, 119.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 283, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (255.33, 102.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700528 reads in 138.770 CPU sec, 4.424 real sec
[M::mem_process_seqs] Processed 699540 reads in 88.672 CPU sec, 2.802 real sec
[M::process] read 700004 sequences (100000091 bp) in 2.103 CPU sec, 2.215 real sec...
sorting block 73, #reads = 1092786 mem = 536878864 peak = 1085161472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291209, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.62, 119.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704552 sequences (100000298 bp) in 2.708 CPU sec, 2.883 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291302, 8, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 219, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638)
[M::mem_pestat] mean and std.dev: (243.94, 117.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 361, 1175)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3167)
[M::mem_pestat] mean and std.dev: (423.64, 394.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4163)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699568 reads in 147.066 CPU sec, 4.698 real sec
[M::mem_process_seqs] Processed 700004 reads in 95.756 CPU sec, 3.033 real sec
sorting block 74, #reads = 1091864 mem = 536877548 peak = 1085161472
[M::process] read 709062 sequences (100000118 bp) in 2.317 CPU sec, 2.475 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292073, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 209, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608)
[M::mem_pestat] mean and std.dev: (233.64, 111.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 761)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 708822 sequences (100000275 bp) in 2.444 CPU sec, 2.587 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 294327, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 201, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574)
[M::mem_pestat] mean and std.dev: (224.00, 105.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 717)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 704552 reads in 163.617 CPU sec, 5.213 real sec
sorting block 75, #reads = 1088884 mem = 536878371 peak = 1085161472
[M::mem_process_seqs] Processed 709062 reads in 101.150 CPU sec, 3.194 real sec
[M::process] read 707700 sequences (100000030 bp) in 1.805 CPU sec, 1.839 real sec...
sorting block 76, #reads = 1087530 mem = 536879870 peak = 1085161472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 294557, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 201, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574)
[M::mem_pestat] mean and std.dev: (224.25, 105.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 717)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 259, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (277.50, 107.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] skip orientation RR
[M::process] read 706582 sequences (100000005 bp) in 2.822 CPU sec, 2.992 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 294524, 8, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 338, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (328.50, 189.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 203, 292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584)
[M::mem_pestat] mean and std.dev: (226.88, 107.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 708822 reads in 158.640 CPU sec, 5.046 real sec
[M::process] read 704282 sequences (100000060 bp) in 1.675 CPU sec, 1.687 real sec...
[M::mem_process_seqs] Processed 707700 reads in 99.377 CPU sec, 3.138 real sec
sorting block 77, #reads = 1091525 mem = 536876774 peak = 1085161472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 293745, 6, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 206, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595)
[M::mem_pestat] mean and std.dev: (229.98, 109.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 744)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 301, 6545)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 19107)
[M::mem_pestat] mean and std.dev: (1972.27, 2800.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 25388)
[M::mem_pestat] skip orientation RR
[M::process] read 703646 sequences (100000016 bp) in 2.412 CPU sec, 2.541 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 293074, 8, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (235.62, 113.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (126, 637, 670)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1758)
[M::mem_pestat] mean and std.dev: (477.67, 300.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2302)
[M::mem_pestat] skip orientation RR
[M::process] read 703136 sequences (100000042 bp) in 1.590 CPU sec, 1.592 real sec...
[M::mem_process_seqs] Processed 706582 reads in 153.789 CPU sec, 4.892 real sec
[M::mem_process_seqs] Processed 704282 reads in 94.312 CPU sec, 2.984 real sec
sorting block 78, #reads = 1090722 mem = 536878407 peak = 1085161472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 293500, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 212, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (237.05, 114.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 187, 229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 379)
[M::mem_pestat] mean and std.dev: (180.62, 30.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 454)
[M::mem_pestat] skip orientation RR
[M::process] read 702942 sequences (100000071 bp) in 2.605 CPU sec, 2.732 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 293066, 5, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (233, 353, 2241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6257)
[M::mem_pestat] mean and std.dev: (1033.73, 1410.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8265)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (239.34, 115.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 703646 reads in 142.484 CPU sec, 4.530 real sec
[M::process] read 702570 sequences (100000124 bp) in 1.606 CPU sec, 1.623 real sec...
sorting block 79, #reads = 1092715 mem = 536880581 peak = 1085161472
[M::mem_process_seqs] Processed 703136 reads in 89.379 CPU sec, 2.823 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292949, 2, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (239.94, 116.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 331, 624)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1448)
[M::mem_pestat] mean and std.dev: (334.07, 167.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1860)
[M::mem_pestat] skip orientation RR
sorting block 80, #reads = 1093232 mem = 536878858 peak = 1109307392
[M::process] read 700942 sequences (100000042 bp) in 2.525 CPU sec, 2.666 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292377, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (241.32, 117.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702942 reads in 140.139 CPU sec, 4.456 real sec
[M::process] read 700646 sequences (100000017 bp) in 1.694 CPU sec, 1.724 real sec...
[M::mem_process_seqs] Processed 702570 reads in 89.270 CPU sec, 2.810 real sec
sorting block 81, #reads = 1093126 mem = 536888146 peak = 1109307392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291609, 4, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 275, 1335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3625)
[M::mem_pestat] mean and std.dev: (571.56, 656.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4770)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (245.12, 119.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 699846 sequences (100000017 bp) in 2.519 CPU sec, 2.669 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292218, 2, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (123, 151, 282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (185.40, 77.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 759)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 219, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (245.13, 118.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 700942 reads in 138.547 CPU sec, 4.408 real sec
[M::mem_process_seqs] Processed 700646 reads in 88.066 CPU sec, 2.774 real sec
[M::process] read 699044 sequences (100000127 bp) in 1.746 CPU sec, 1.783 real sec...
sorting block 82, #reads = 1092594 mem = 536883838 peak = 1109307392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291455, 5, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (249.07, 121.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 2156, 2461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6861)
[M::mem_pestat] mean and std.dev: (1335.86, 1015.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9061)
[M::mem_pestat] skip orientation RR
[M::process] read 699112 sequences (100000256 bp) in 2.322 CPU sec, 2.485 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291308, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.78, 122.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699846 reads in 137.411 CPU sec, 4.385 real sec
[M::mem_process_seqs] Processed 699044 reads in 87.717 CPU sec, 2.773 real sec
sorting block 83, #reads = 1093179 mem = 536880574 peak = 1109307392
[M::process] read 697962 sequences (100000069 bp) in 1.795 CPU sec, 1.851 real sec...
[M::process] read 12768 sequences (1819397 bp) in 0.362 CPU sec, 0.366 real sec...
sorting block 84, #reads = 1091333 mem = 536878556 peak = 1109307392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291322, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.23, 120.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 272, 2754)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7718)
[M::mem_pestat] mean and std.dev: (1161.91, 1252.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10200)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290990, 5, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (256, 352, 750)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1738)
[M::mem_pestat] mean and std.dev: (447.91, 339.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2232)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.91, 120.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699112 reads in 134.830 CPU sec, 4.376 real sec
[M::mem_process_seqs] Processed 697962 reads in 84.946 CPU sec, 2.754 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 5326, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (244.05, 117.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 12768 reads in 1.840 CPU sec, 0.066 real sec
sorting block 85, #reads = 1091041 mem = 536878250 peak = 1109307392
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 93890738
overall: 70065573888 mem 11381.624 user 360.581 sys 389.460 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1047_JG-T1-TDNA-1_A23MHWWLT4_1.L006\tPU:L006\tSM:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tLB:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0003.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0003.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 389.508 sec; CPU: 11742.221 sec
sorting block 86, #reads = 386500 mem = 190419005 peak = 1109307392
uniq header count 1
merging 87 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 5197908723
output reads: 94369169
bam_mem_sort: 87 calls 32.397 user 0.946 sys 33.549 real
bam_write: 87 calls 54.164 user 2.426 sys 56.983 real
execute: 1 calls 18.223 user 1.183 sys 416.943 real
merge_files: 1 calls 18.193 user 1.174 sys 26.954 real
parse_chunk: 1382 calls 45.483 user 10.232 sys 56.781 real
read_chunk: 3733 calls 36.581 user 36.297 sys 337.054 real
sort_block: 1 calls 28.746 user 35.914 sys 325.331 real
write_chunk: 2349 calls 17.787 user 0.975 sys 26.118 real
overall: 2979168256 mem 1046.743 user 57.812 sys 416.964 real
ls: cannot access '*.cram': No such file or directory