.command.err - Workdir - DAQ:SENTIEON_BWAMEM (1029_N5D-T1-TDNA-1_A23MHWWLT4_1;L004;0001)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/2a/7815bf6ab429bda3172caba1f0267e/.command.err
Size: 137.5 KB
Task: DAQ:SENTIEON_BWAMEM (1029_N5D-T1-TDNA-1_A23MHWWLT4_1;L004;0001)
Attempt
Content

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1029_N5D-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1748.145 CPU sec, 479.090 real sec
[M::process] read 685908 sequences (100000154 bp) in 0.952 CPU sec, 0.958 real sec...
[M::process] read 685204 sequences (100000299 bp) in 1.206 CPU sec, 1.261 real sec...
[M::process] read 683662 sequences (100000231 bp) in 1.175 CPU sec, 1.188 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 279442, 10, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 232, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (247.89, 119.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 320, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (339.42, 167.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 1475, 1908)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5386)
[M::mem_pestat] mean and std.dev: (1180.20, 941.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7125)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 265, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (299.32, 153.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 682816 sequences (100000300 bp) in 1.129 CPU sec, 1.131 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 279432, 6, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 186, 596)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1514)
[M::mem_pestat] mean and std.dev: (343.33, 357.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1973)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 325, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (343.13, 167.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 267, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (310.96, 192.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 278175, 3, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 243, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1113)
[M::mem_pestat] mean and std.dev: (284.12, 172.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1431)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 334, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (351.57, 171.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 273, 568)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1310)
[M::mem_pestat] mean and std.dev: (342.75, 235.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1681)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685908 reads in 271.415 CPU sec, 8.755 real sec
[M::mem_process_seqs] Processed 685204 reads in 185.655 CPU sec, 6.034 real sec
[M::mem_process_seqs] Processed 683662 reads in 203.949 CPU sec, 6.616 real sec
sorting block 0, #reads = 1071930 mem = 536877971 peak = 604590080
[M::process] read 682242 sequences (100000083 bp) in 1.988 CPU sec, 2.088 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 278506, 7, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 227, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (254.36, 143.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 342, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (358.53, 173.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 290, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860)
[M::mem_pestat] mean and std.dev: (321.02, 154.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682410 sequences (100000164 bp) in 1.738 CPU sec, 1.735 real sec...
[M::process] read 682578 sequences (100000112 bp) in 1.610 CPU sec, 1.615 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 278273, 12, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (231, 329, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864)
[M::mem_pestat] mean and std.dev: (314.47, 139.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1075)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 342, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (359.57, 173.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (817, 1367, 2572)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6082)
[M::mem_pestat] mean and std.dev: (1534.08, 1013.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7837)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (332, 377, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (180, 560)
[M::mem_pestat] mean and std.dev: (370.60, 68.09)
[M::mem_pestat] low and high boundaries for proper pairs: (98, 643)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682816 reads in 171.076 CPU sec, 5.407 real sec
sorting block 1, #reads = 1070403 mem = 536882245 peak = 790188032
[M::mem_process_seqs] Processed 682242 reads in 108.978 CPU sec, 3.448 real sec
sorting block 2, #reads = 1069619 mem = 536878103 peak = 904323072
[M::process] read 682076 sequences (100000029 bp) in 1.963 CPU sec, 2.010 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 278069, 10, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 279, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1179)
[M::mem_pestat] mean and std.dev: (313.69, 193.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1506)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 340, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (357.00, 172.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 456, 1004)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2960)
[M::mem_pestat] mean and std.dev: (384.67, 383.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3938)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 271, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (315.41, 154.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 681766 sequences (100000283 bp) in 1.710 CPU sec, 1.706 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 277821, 14, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 301, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762)
[M::mem_pestat] mean and std.dev: (287.52, 115.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 956)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 339, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (356.90, 172.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1068, 1270, 2498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5358)
[M::mem_pestat] mean and std.dev: (1283.83, 929.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6788)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 360, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1124)
[M::mem_pestat] mean and std.dev: (403.87, 243.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1428)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 278078, 8, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 236, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (245.84, 99.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 337, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (354.09, 171.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 255, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (237.76, 90.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 732)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682410 reads in 246.584 CPU sec, 7.871 real sec
[M::mem_process_seqs] Processed 682076 reads in 184.811 CPU sec, 5.937 real sec
[M::mem_process_seqs] Processed 682578 reads in 202.786 CPU sec, 6.528 real sec
sorting block 3, #reads = 1070032 mem = 536878847 peak = 904323072
[M::process] read 681028 sequences (100000073 bp) in 2.188 CPU sec, 2.306 real sec...
sorting block 4, #reads = 1070526 mem = 536880195 peak = 904323072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 277776, 4, 53)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (231, 258, 825)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2013)
[M::mem_pestat] mean and std.dev: (343.03, 253.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2607)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 342, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (358.86, 172.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 386, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1198)
[M::mem_pestat] mean and std.dev: (379.56, 225.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1542)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680680 sequences (100000246 bp) in 1.770 CPU sec, 1.795 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 277347, 13, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 272, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.79, 108.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 880)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 344, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (360.86, 171.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1579, 2355, 4928)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11626)
[M::mem_pestat] mean and std.dev: (2929.46, 2220.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14975)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 311, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (256.20, 118.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 679994 sequences (100000071 bp) in 1.510 CPU sec, 1.501 real sec...
[M::mem_process_seqs] Processed 681766 reads in 176.765 CPU sec, 5.618 real sec
[M::mem_process_seqs] Processed 681028 reads in 111.538 CPU sec, 3.529 real sec
sorting block 5, #reads = 1069462 mem = 536880542 peak = 904323072
[M::process] read 683994 sequences (100000043 bp) in 1.733 CPU sec, 1.823 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 277312, 3, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 193, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (280.55, 170.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 348, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (363.38, 172.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 255, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553)
[M::mem_pestat] mean and std.dev: (248.78, 87.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 676)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685108 sequences (100000134 bp) in 1.290 CPU sec, 1.291 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 277565, 2, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 296, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1151)
[M::mem_pestat] mean and std.dev: (344.24, 205.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1473)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 354, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (369.76, 175.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 338, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (375.32, 238.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1329)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278629, 11, 55)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 218, 535)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1313)
[M::mem_pestat] mean and std.dev: (376.12, 339.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1736)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 334, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (351.75, 172.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (684, 735, 1068)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1836)
[M::mem_pestat] mean and std.dev: (590.44, 299.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2220)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 286, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1163)
[M::mem_pestat] mean and std.dev: (354.50, 241.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1485)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680680 reads in 242.117 CPU sec, 7.760 real sec
[M::mem_process_seqs] Processed 679994 reads in 179.096 CPU sec, 5.770 real sec
sorting block 6, #reads = 1069474 mem = 536881057 peak = 904323072
[M::mem_process_seqs] Processed 683994 reads in 196.145 CPU sec, 6.335 real sec
sorting block 7, #reads = 1069942 mem = 536877902 peak = 904323072
[M::process] read 684394 sequences (100000123 bp) in 2.391 CPU sec, 2.544 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 279219, 3, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (197, 306, 600)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1406)
[M::mem_pestat] mean and std.dev: (344.74, 228.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1809)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 329, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (346.98, 170.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 272, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (245.00, 107.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683274 sequences (100000296 bp) in 1.943 CPU sec, 1.984 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 278546, 3, 53)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 209, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (223.26, 117.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 335, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (351.45, 171.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 296, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (308.76, 103.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 902)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685108 reads in 174.391 CPU sec, 5.540 real sec
[M::process] read 682450 sequences (100000137 bp) in 1.753 CPU sec, 1.781 real sec...
[M::process] read 682538 sequences (100000184 bp) in 1.754 CPU sec, 1.755 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 278278, 4, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (238, 334, 604)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1336)
[M::mem_pestat] mean and std.dev: (386.12, 203.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1702)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 341, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (356.79, 173.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 279, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (292.48, 172.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683274 reads in 93.967 CPU sec, 3.159 real sec
sorting block 8, #reads = 1074176 mem = 536881162 peak = 904323072
[M::mem_process_seqs] Processed 684394 reads in 232.822 CPU sec, 7.546 real sec
sorting block 9, #reads = 1073581 mem = 536879119 peak = 934260736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 278563, 5, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 264, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543)
[M::mem_pestat] mean and std.dev: (253.14, 81.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 659)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 345, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (361.36, 174.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 287, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (287.67, 136.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682484 sequences (100000128 bp) in 1.948 CPU sec, 2.117 real sec...
[M::process] read 682994 sequences (100000288 bp) in 1.720 CPU sec, 1.721 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 277902, 7, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 231, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (249.98, 128.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 345, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (361.59, 175.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 315, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1096)
[M::mem_pestat] mean and std.dev: (334.30, 155.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1387)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682450 reads in 196.004 CPU sec, 6.290 real sec
[M::mem_process_seqs] Processed 682538 reads in 115.820 CPU sec, 3.683 real sec
sorting block 10, #reads = 1071991 mem = 536877650 peak = 934260736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 278462, 8, 62)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 175, 240)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456)
[M::mem_pestat] mean and std.dev: (187.40, 84.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 564)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 340, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (357.64, 173.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 308, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863)
[M::mem_pestat] mean and std.dev: (322.07, 153.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1082)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682288 sequences (100000181 bp) in 2.278 CPU sec, 2.366 real sec...
[M::process] read 681838 sequences (100000022 bp) in 1.790 CPU sec, 1.787 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 278498, 5, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 200, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549)
[M::mem_pestat] mean and std.dev: (200.25, 68.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 678)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 334, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (351.37, 169.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 276, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869)
[M::mem_pestat] mean and std.dev: (303.50, 160.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1106)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682484 reads in 174.836 CPU sec, 5.554 real sec
sorting block 11, #reads = 1071108 mem = 536881180 peak = 934260736
[M::mem_process_seqs] Processed 682994 reads in 111.454 CPU sec, 3.548 real sec
[M::process] read 681454 sequences (100000032 bp) in 2.213 CPU sec, 2.315 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 278200, 5, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (203, 353, 622)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1460)
[M::mem_pestat] mean and std.dev: (385.06, 258.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1879)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 337, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (354.24, 170.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 284, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1120)
[M::mem_pestat] mean and std.dev: (288.48, 154.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1438)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681014 sequences (100000121 bp) in 1.730 CPU sec, 1.726 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 278266, 8, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 275, 1162)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3138)
[M::mem_pestat] mean and std.dev: (698.50, 875.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4202)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 340, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (357.13, 171.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 267, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (260.15, 108.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278311, 6, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (114, 254, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762)
[M::mem_pestat] mean and std.dev: (243.26, 144.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 978)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 346, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (362.28, 174.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 262, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (258.91, 97.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682288 reads in 248.110 CPU sec, 7.937 real sec
sorting block 12, #reads = 1070240 mem = 536879002 peak = 934260736
[M::mem_process_seqs] Processed 681838 reads in 186.358 CPU sec, 5.983 real sec
[M::mem_process_seqs] Processed 681454 reads in 204.295 CPU sec, 6.583 real sec
sorting block 13, #reads = 1070324 mem = 536879937 peak = 934260736
[M::process] read 683330 sequences (100000295 bp) in 2.463 CPU sec, 2.586 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 278450, 3, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (205, 278, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1180)
[M::mem_pestat] mean and std.dev: (326.72, 206.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1505)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 349, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (365.64, 176.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 255, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (282.18, 145.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686768 sequences (100000287 bp) in 2.090 CPU sec, 2.134 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278833, 6, 50)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 337, 1220)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3334)
[M::mem_pestat] mean and std.dev: (806.82, 939.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4567)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 339, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (357.39, 175.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 324, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1123)
[M::mem_pestat] mean and std.dev: (369.86, 217.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1434)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681014 reads in 172.903 CPU sec, 5.505 real sec
[M::mem_process_seqs] Processed 683330 reads in 114.933 CPU sec, 3.618 real sec
sorting block 14, #reads = 1068918 mem = 536879481 peak = 934260736
[M::process] read 685494 sequences (100000024 bp) in 2.302 CPU sec, 2.386 real sec...
sorting block 15, #reads = 1069214 mem = 536878053 peak = 934260736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 279319, 6, 51)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 282, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (255.68, 109.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 966)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 319, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (336.73, 165.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 335, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1100)
[M::mem_pestat] mean and std.dev: (328.15, 167.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1396)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683836 sequences (100000006 bp) in 2.253 CPU sec, 2.316 real sec...
[M::process] read 683072 sequences (100000109 bp) in 1.800 CPU sec, 1.797 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 278885, 9, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 174, 209)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 341)
[M::mem_pestat] mean and std.dev: (173.17, 51.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 407)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 325, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (340.75, 164.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 270, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (283.30, 149.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686768 reads in 178.228 CPU sec, 5.654 real sec
[M::mem_process_seqs] Processed 685494 reads in 113.786 CPU sec, 3.616 real sec
sorting block 16, #reads = 1072277 mem = 536879033 peak = 934260736
[M::process] read 682658 sequences (100000189 bp) in 2.046 CPU sec, 2.154 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 278627, 2, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 280, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (289.85, 168.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 334, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (348.19, 166.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 299, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (293.82, 118.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682760 sequences (100000285 bp) in 1.808 CPU sec, 1.812 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278167, 6, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 197, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (231.90, 146.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 934)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 338, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (352.49, 167.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 264, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (312.22, 123.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 278223, 2, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (215, 271, 555)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1235)
[M::mem_pestat] mean and std.dev: (336.12, 239.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1575)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 341, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (355.90, 170.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 239, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (265.18, 101.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683836 reads in 244.508 CPU sec, 7.821 real sec
[M::mem_process_seqs] Processed 683072 reads in 178.008 CPU sec, 5.699 real sec
sorting block 17, #reads = 1071664 mem = 536885941 peak = 934260736
[M::process] read 682472 sequences (100000059 bp) in 2.382 CPU sec, 2.455 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 278208, 3, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 266, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (282.42, 141.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 339, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (354.07, 169.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 234, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (248.14, 124.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682760 reads in 94.341 CPU sec, 2.982 real sec
sorting block 18, #reads = 1069640 mem = 536882793 peak = 934260736
[M::mem_process_seqs] Processed 682658 reads in 331.724 CPU sec, 10.591 real sec
[M::process] read 682130 sequences (100000184 bp) in 2.376 CPU sec, 2.497 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 277796, 14, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (123, 173, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (242.17, 157.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 873)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 337, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (353.36, 168.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 199, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1083)
[M::mem_pestat] mean and std.dev: (252.25, 197.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1384)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 317, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (300.78, 123.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 682064 sequences (100000009 bp) in 2.182 CPU sec, 2.248 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 277565, 7, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 273, 620)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1570)
[M::mem_pestat] mean and std.dev: (371.09, 288.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2045)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 339, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (354.23, 169.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 328, 683)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1653)
[M::mem_pestat] mean and std.dev: (459.82, 385.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2138)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682472 reads in 189.801 CPU sec, 5.978 real sec
sorting block 19, #reads = 1071185 mem = 536877253 peak = 934260736
[M::mem_process_seqs] Processed 682130 reads in 112.431 CPU sec, 3.532 real sec
[M::process] read 681712 sequences (100000002 bp) in 1.934 CPU sec, 1.970 real sec...
sorting block 20, #reads = 1068621 mem = 536880299 peak = 940584960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 277382, 4, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 326, 1124)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3022)
[M::mem_pestat] mean and std.dev: (555.94, 660.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3971)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 337, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (353.15, 168.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 351, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1064)
[M::mem_pestat] mean and std.dev: (355.07, 177.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1342)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681622 sequences (100000223 bp) in 2.524 CPU sec, 2.625 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 277753, 7, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 251, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1169)
[M::mem_pestat] mean and std.dev: (310.67, 194.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1503)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 683608 sequences (100000131 bp) in 1.800 CPU sec, 1.791 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (221, 342, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (358.80, 171.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 282, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093)
[M::mem_pestat] mean and std.dev: (315.39, 177.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1397)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682064 reads in 172.398 CPU sec, 5.475 real sec
[M::mem_process_seqs] Processed 681712 reads in 107.368 CPU sec, 3.399 real sec
sorting block 21, #reads = 1068671 mem = 536886739 peak = 940584960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 277825, 3, 53)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 293, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (330.82, 207.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 345, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (362.00, 174.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 284, 773)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1923)
[M::mem_pestat] mean and std.dev: (494.31, 496.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2498)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685596 sequences (100000160 bp) in 1.955 CPU sec, 2.054 real sec...
[M::process] read 684994 sequences (100000019 bp) in 1.370 CPU sec, 1.361 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278766, 3, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 245, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (254.97, 147.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 334, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (352.36, 172.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 272, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1065)
[M::mem_pestat] mean and std.dev: (310.22, 149.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1354)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681622 reads in 152.072 CPU sec, 4.839 real sec
[M::mem_process_seqs] Processed 683608 reads in 99.778 CPU sec, 3.178 real sec
sorting block 22, #reads = 1067311 mem = 536878660 peak = 940584960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 278502, 1, 52)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (215, 363, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881)
[M::mem_pestat] mean and std.dev: (334.39, 146.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1103)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 324, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (341.13, 166.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 305, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (277.98, 86.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 869)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683794 sequences (100000095 bp) in 1.940 CPU sec, 2.037 real sec...
[M::process] read 683230 sequences (100000067 bp) in 1.420 CPU sec, 1.420 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 278844, 11, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 211, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (260.52, 131.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 328, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (344.72, 167.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (651, 651, 1869)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4305)
[M::mem_pestat] mean and std.dev: (934.09, 582.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5523)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 346, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (336.52, 153.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685596 reads in 158.169 CPU sec, 5.034 real sec
[M::mem_process_seqs] Processed 684994 reads in 104.565 CPU sec, 3.324 real sec
sorting block 23, #reads = 1069394 mem = 536878234 peak = 940584960
sorting block 24, #reads = 1072676 mem = 536879307 peak = 940584960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 277852, 5, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 231, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (232.69, 86.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 740)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 334, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (349.61, 168.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (284, 370, 663)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1421)
[M::mem_pestat] mean and std.dev: (429.49, 217.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1800)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683012 sequences (100000085 bp) in 2.173 CPU sec, 2.258 real sec...
[M::process] read 683966 sequences (100000000 bp) in 1.450 CPU sec, 1.450 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 277783, 8, 66)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 203, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (199.22, 76.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 337, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (352.16, 168.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 356, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1162)
[M::mem_pestat] mean and std.dev: (370.28, 215.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1485)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683794 reads in 166.334 CPU sec, 5.301 real sec
[M::mem_process_seqs] Processed 683230 reads in 108.794 CPU sec, 3.457 real sec
sorting block 25, #reads = 1070459 mem = 536879394 peak = 940584960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278149, 4, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 290, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053)
[M::mem_pestat] mean and std.dev: (320.84, 186.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1346)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 339, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (353.82, 168.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 211, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (252.97, 163.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683352 sequences (100000092 bp) in 2.318 CPU sec, 2.403 real sec...
[M::process] read 682646 sequences (100000048 bp) in 1.789 CPU sec, 1.790 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 278393, 10, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 244, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1206)
[M::mem_pestat] mean and std.dev: (251.29, 127.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1552)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 331, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (348.39, 169.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (410, 763, 981)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2123)
[M::mem_pestat] mean and std.dev: (632.20, 315.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2694)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 253, 699)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1743)
[M::mem_pestat] mean and std.dev: (387.13, 295.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2265)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683012 reads in 164.411 CPU sec, 5.250 real sec
[M::mem_process_seqs] Processed 683966 reads in 107.946 CPU sec, 3.425 real sec
sorting block 26, #reads = 1069948 mem = 536879864 peak = 940584960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 277803, 3, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 249, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (267.55, 126.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 331, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (346.85, 165.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (137, 243, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866)
[M::mem_pestat] mean and std.dev: (269.77, 141.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682626 sequences (100000271 bp) in 2.311 CPU sec, 2.399 real sec...
[M::process] read 682050 sequences (100000066 bp) in 1.790 CPU sec, 1.792 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 277482, 13, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 381, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (328.50, 168.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 330, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (346.07, 163.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (670, 670, 3962)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10546)
[M::mem_pestat] mean and std.dev: (1919.92, 1614.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 13838)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 389, 567)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1231)
[M::mem_pestat] mean and std.dev: (362.86, 174.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1563)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683352 reads in 172.857 CPU sec, 5.580 real sec
[M::mem_process_seqs] Processed 682646 reads in 112.031 CPU sec, 3.564 real sec
sorting block 27, #reads = 1069367 mem = 536889080 peak = 940584960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 277914, 8, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 328, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865)
[M::mem_pestat] mean and std.dev: (318.27, 175.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1095)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 329, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (345.41, 162.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 203, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (284.25, 179.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681714 sequences (100000012 bp) in 2.255 CPU sec, 2.338 real sec...
[M::process] read 684906 sequences (100000028 bp) in 1.440 CPU sec, 1.443 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 277529, 9, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 241, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (237.15, 115.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 335, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (350.45, 165.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 293, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (295.92, 138.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682626 reads in 157.543 CPU sec, 5.014 real sec
sorting block 28, #reads = 1067646 mem = 536878174 peak = 940584960
[M::mem_process_seqs] Processed 682050 reads in 98.713 CPU sec, 3.141 real sec
sorting block 29, #reads = 1068053 mem = 536879679 peak = 940584960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 277864, 4, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 359, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (326.13, 127.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 341, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (357.30, 170.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 334, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (341.00, 152.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 688080 sequences (100000088 bp) in 2.086 CPU sec, 2.157 real sec...
[M::process] read 685436 sequences (100000162 bp) in 1.390 CPU sec, 1.397 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 279038, 7, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 230, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (246.06, 102.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 325, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (345.68, 171.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 280, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (277.72, 165.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681714 reads in 151.729 CPU sec, 4.830 real sec
[M::mem_process_seqs] Processed 684906 reads in 101.818 CPU sec, 3.236 real sec
sorting block 30, #reads = 1068352 mem = 536877059 peak = 940584960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 280373, 9, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 197, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (234.94, 105.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 984)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 309, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (333.85, 169.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 339, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (289.44, 137.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684318 sequences (100000189 bp) in 2.038 CPU sec, 2.126 real sec...
[M::process] read 683744 sequences (100000021 bp) in 1.408 CPU sec, 1.408 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 280104, 8, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 271, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881)
[M::mem_pestat] mean and std.dev: (289.80, 186.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 327, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (347.86, 174.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (126, 208, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (212.46, 94.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688080 reads in 151.042 CPU sec, 4.801 real sec
[M::mem_process_seqs] Processed 685436 reads in 96.684 CPU sec, 3.071 real sec
sorting block 31, #reads = 1070311 mem = 536880657 peak = 940584960
[M::process] read 683272 sequences (100000008 bp) in 1.981 CPU sec, 2.058 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 279678, 6, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 252, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (262.93, 158.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1332)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 332, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (350.70, 172.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 468, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (357.19, 148.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683106 sequences (100000286 bp) in 1.410 CPU sec, 1.413 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278577, 5, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (219, 348, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1152)
[M::mem_pestat] mean and std.dev: (383.32, 205.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1463)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 337, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (354.76, 173.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 258, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (243.52, 91.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684318 reads in 153.995 CPU sec, 5.127 real sec
sorting block 32, #reads = 1071781 mem = 536879651 peak = 940584960
[M::mem_process_seqs] Processed 683744 reads in 96.714 CPU sec, 3.079 real sec
sorting block 33, #reads = 1068540 mem = 536880258 peak = 961859584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 278730, 10, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 220, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (249.67, 133.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 337, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (353.82, 171.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (107, 551, 616)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1634)
[M::mem_pestat] mean and std.dev: (495.20, 434.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2232)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 253, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (269.82, 132.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 682848 sequences (100000178 bp) in 1.945 CPU sec, 2.035 real sec...
[M::process] read 682566 sequences (100000201 bp) in 1.410 CPU sec, 1.406 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 278775, 7, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 233, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (277.88, 170.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 339, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (355.61, 171.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 279, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (295.61, 182.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683272 reads in 154.986 CPU sec, 4.930 real sec
[M::mem_process_seqs] Processed 683106 reads in 100.801 CPU sec, 3.204 real sec
sorting block 34, #reads = 1067423 mem = 536882996 peak = 961859584
[M::process] read 682324 sequences (100000045 bp) in 1.993 CPU sec, 2.063 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 278906, 5, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 283, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (313.67, 203.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 336, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (353.24, 170.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 252, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (254.15, 93.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682480 sequences (100000088 bp) in 1.400 CPU sec, 1.407 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 278277, 3, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 285, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (284.78, 126.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 919)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 338, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (354.89, 170.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 284, 675)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1589)
[M::mem_pestat] mean and std.dev: (385.76, 271.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2046)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682848 reads in 155.923 CPU sec, 5.053 real sec
[M::mem_process_seqs] Processed 682566 reads in 100.604 CPU sec, 3.209 real sec
sorting block 35, #reads = 1065788 mem = 536878418 peak = 961859584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278282, 4, 56)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 298, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (303.74, 173.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 338, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (354.12, 169.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 277, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (299.19, 163.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681804 sequences (100000133 bp) in 2.139 CPU sec, 2.272 real sec...
[M::process] read 690818 sequences (100000149 bp) in 1.430 CPU sec, 1.436 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 278937, 5, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 284, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737)
[M::mem_pestat] mean and std.dev: (264.82, 125.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 338, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (355.95, 171.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 244, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (267.32, 138.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682324 reads in 153.391 CPU sec, 4.908 real sec
sorting block 36, #reads = 1065747 mem = 536881262 peak = 961859584
[M::mem_process_seqs] Processed 682480 reads in 98.960 CPU sec, 3.147 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 277998, 5, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 267, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (269.73, 152.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 344, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (361.40, 174.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 239, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (245.26, 98.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 690286 sequences (100000162 bp) in 2.201 CPU sec, 2.259 real sec...
[M::process] read 687922 sequences (100000199 bp) in 1.420 CPU sec, 1.425 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 281593, 14, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 210, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581)
[M::mem_pestat] mean and std.dev: (208.30, 107.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 729)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 292, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (317.95, 162.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (734, 1449, 8583)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 24281)
[M::mem_pestat] mean and std.dev: (3327.79, 3446.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 32130)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 339, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1132)
[M::mem_pestat] mean and std.dev: (347.38, 194.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1441)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681804 reads in 160.913 CPU sec, 5.145 real sec
sorting block 37, #reads = 1067174 mem = 536876826 peak = 961859584
[M::mem_process_seqs] Processed 690818 reads in 103.720 CPU sec, 3.294 real sec
sorting block 38, #reads = 1066654 mem = 536879440 peak = 1005629440
[M::process] read 686818 sequences (100000162 bp) in 2.149 CPU sec, 2.209 real sec...
[M::process] read 685982 sequences (100000011 bp) in 1.420 CPU sec, 1.424 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 281434, 5, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 233, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1230)
[M::mem_pestat] mean and std.dev: (288.00, 192.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1590)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 309, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (329.74, 163.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 208, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (231.95, 113.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687922 reads in 85.451 CPU sec, 2.713 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 281885, 4, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 280, 648)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1570)
[M::mem_pestat] mean and std.dev: (363.00, 270.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2031)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 295, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (318.10, 159.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 257, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (273.49, 126.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 280931, 2, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 260, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (278.49, 196.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 317, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (335.82, 165.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 275, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (45, 475)
[M::mem_pestat] mean and std.dev: (256.21, 63.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 561)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684554 sequences (100000128 bp) in 1.933 CPU sec, 1.936 real sec...
[M::mem_process_seqs] Processed 690286 reads in 245.261 CPU sec, 7.780 real sec
sorting block 39, #reads = 1067829 mem = 536878581 peak = 1005629440
[M::mem_process_seqs] Processed 686818 reads in 119.126 CPU sec, 3.792 real sec
sorting block 40, #reads = 1067387 mem = 536880726 peak = 1005629440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 280080, 4, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (108, 194, 282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (210.89, 115.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 804)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 323, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (340.02, 166.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 243, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (295.12, 141.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683652 sequences (100000066 bp) in 2.234 CPU sec, 2.348 real sec...
[M::process] read 683108 sequences (100000105 bp) in 1.380 CPU sec, 1.382 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 279090, 10, 53)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 319, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (266.66, 96.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 330, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (346.60, 168.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (236, 1446, 1702)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4634)
[M::mem_pestat] mean and std.dev: (1113.33, 787.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6100)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 332, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (308.22, 140.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685982 reads in 159.380 CPU sec, 5.065 real sec
[M::mem_process_seqs] Processed 684554 reads in 103.777 CPU sec, 3.299 real sec
sorting block 41, #reads = 1065777 mem = 536877986 peak = 1005629440
[M::process] read 682670 sequences (100000131 bp) in 2.072 CPU sec, 2.176 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 278858, 6, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 244, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (263.03, 138.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 332, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (349.88, 169.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 265, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (281.06, 115.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681956 sequences (100000226 bp) in 1.408 CPU sec, 1.403 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 278646, 3, 57)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 410, 566)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1346)
[M::mem_pestat] mean and std.dev: (385.35, 264.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1736)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 336, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (354.13, 171.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 299, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (311.25, 137.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683652 reads in 157.341 CPU sec, 5.001 real sec
sorting block 42, #reads = 1064630 mem = 536881342 peak = 1005629440
[M::process] read 682244 sequences (100000029 bp) in 1.602 CPU sec, 1.604 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 278199, 2, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (201, 262, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (247.11, 80.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 737)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 335, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (353.47, 170.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 275, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (329.74, 186.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683108 reads in 172.164 CPU sec, 5.464 real sec
[M::mem_process_seqs] Processed 682670 reads in 195.846 CPU sec, 6.248 real sec
sorting block 43, #reads = 1065916 mem = 536877867 peak = 1005629440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278486, 12, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 298, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1117)
[M::mem_pestat] mean and std.dev: (352.91, 251.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1433)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 342, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (359.68, 173.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (112, 1318, 2827)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8257)
[M::mem_pestat] mean and std.dev: (1793.33, 2136.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10972)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 279, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (303.88, 125.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 690034 sequences (100000215 bp) in 1.990 CPU sec, 2.102 real sec...
[M::process] read 687362 sequences (100000055 bp) in 1.400 CPU sec, 1.393 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 278445, 7, 48)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 281, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (257.72, 130.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 340, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (360.29, 176.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 236, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (282.41, 171.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1320)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681956 reads in 156.665 CPU sec, 4.983 real sec
[M::mem_process_seqs] Processed 682244 reads in 103.815 CPU sec, 3.305 real sec
sorting block 44, #reads = 1067003 mem = 536882139 peak = 1005629440
[M::process] read 685578 sequences (100000061 bp) in 1.966 CPU sec, 2.071 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 280928, 3, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 189, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1118)
[M::mem_pestat] mean and std.dev: (228.00, 132.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1445)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 298, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (321.59, 162.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 341, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1115)
[M::mem_pestat] mean and std.dev: (339.66, 225.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1417)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 690034 reads in 90.420 CPU sec, 3.214 real sec
sorting block 45, #reads = 1065534 mem = 536878307 peak = 1005629440
[M::process] read 685268 sequences (100000185 bp) in 1.907 CPU sec, 2.103 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 280285, 3, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 234, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586)
[M::mem_pestat] mean and std.dev: (215.46, 73.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 724)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 312, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (331.60, 163.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 284, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (305.09, 135.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1292)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685170 sequences (100000291 bp) in 1.398 CPU sec, 1.400 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 279303, 6, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 201, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (192.71, 96.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 321, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (337.72, 164.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 344, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (306.61, 140.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687362 reads in 173.749 CPU sec, 5.567 real sec
[M::mem_process_seqs] Processed 685578 reads in 187.729 CPU sec, 6.022 real sec
sorting block 46, #reads = 1071702 mem = 536879206 peak = 1005629440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 280124, 2, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 247, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (267.37, 159.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 326, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (342.02, 165.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 318, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1242)
[M::mem_pestat] mean and std.dev: (340.45, 232.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1598)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684982 sequences (100000131 bp) in 2.009 CPU sec, 2.124 real sec...
[M::process] read 684240 sequences (100000264 bp) in 1.400 CPU sec, 1.392 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 279847, 1, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 206, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (207.08, 109.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 894)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 325, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (341.63, 164.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 229, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869)
[M::mem_pestat] mean and std.dev: (239.38, 104.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1092)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685268 reads in 158.342 CPU sec, 5.039 real sec
[M::mem_process_seqs] Processed 685170 reads in 99.016 CPU sec, 3.159 real sec
sorting block 47, #reads = 1070097 mem = 536884500 peak = 1005629440
[M::process] read 683696 sequences (100000053 bp) in 1.908 CPU sec, 1.983 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 278851, 1, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 183, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (25, 410)
[M::mem_pestat] mean and std.dev: (207.86, 76.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 512)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 324, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (339.73, 163.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 317, 540)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076)
[M::mem_pestat] mean and std.dev: (364.59, 188.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1344)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683510 sequences (100000215 bp) in 1.410 CPU sec, 1.408 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 278686, 3, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 330, 536)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1252)
[M::mem_pestat] mean and std.dev: (379.22, 290.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1610)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 333, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (349.84, 168.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 302, 585)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1275)
[M::mem_pestat] mean and std.dev: (356.81, 205.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1620)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278531, 6, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 190, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (237.46, 140.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 864)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 330, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (345.95, 166.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 262, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (256.41, 92.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684982 reads in 234.468 CPU sec, 7.702 real sec
sorting block 48, #reads = 1070546 mem = 536879491 peak = 1005629440
[M::mem_process_seqs] Processed 683696 reads in 180.342 CPU sec, 5.786 real sec
[M::mem_process_seqs] Processed 684240 reads in 197.678 CPU sec, 6.355 real sec
sorting block 49, #reads = 1067683 mem = 536881023 peak = 1038675968
[M::process] read 683040 sequences (100000053 bp) in 2.311 CPU sec, 2.481 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 278725, 5, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 277, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (257.85, 95.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 722)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 328, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (346.18, 166.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 202, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (210.97, 69.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 773)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682980 sequences (100000042 bp) in 1.652 CPU sec, 1.754 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 278769, 2, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (209, 232, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (253.93, 97.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 335, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (352.09, 169.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 242, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (275.82, 147.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 691010 sequences (100000220 bp) in 1.400 CPU sec, 1.398 real sec...
[M::mem_process_seqs] Processed 683510 reads in 173.269 CPU sec, 5.526 real sec
sorting block 50, #reads = 1065191 mem = 536881608 peak = 1038675968
[M::mem_process_seqs] Processed 683040 reads in 107.840 CPU sec, 3.444 real sec
sorting block 51, #reads = 1064862 mem = 536881582 peak = 1038675968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 279229, 10, 57)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 274, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (246.58, 101.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 336, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (354.06, 171.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (240, 673, 954)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2382)
[M::mem_pestat] mean and std.dev: (551.60, 393.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3096)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 389, 666)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1478)
[M::mem_pestat] mean and std.dev: (390.60, 213.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1884)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 691120 sequences (100000286 bp) in 2.056 CPU sec, 2.167 real sec...
[M::process] read 688582 sequences (100000200 bp) in 1.420 CPU sec, 1.425 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281846, 10, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 243, 616)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1518)
[M::mem_pestat] mean and std.dev: (330.73, 260.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1969)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 292, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (316.75, 161.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1120, 1951, 1951)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3613)
[M::mem_pestat] mean and std.dev: (1444.11, 770.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4525)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 273, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (263.77, 124.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682980 reads in 173.261 CPU sec, 5.553 real sec
[M::mem_process_seqs] Processed 691010 reads in 112.501 CPU sec, 3.592 real sec
sorting block 52, #reads = 1059545 mem = 536880711 peak = 1038675968
[M::process] read 687418 sequences (100000158 bp) in 1.874 CPU sec, 1.951 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 281721, 5, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485)
[M::mem_pestat] mean and std.dev: (205.20, 83.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 596)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 292, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (312.93, 155.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 258, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (299.41, 152.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686824 sequences (100000301 bp) in 1.428 CPU sec, 1.428 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 281260, 1, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (197, 246, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (278.79, 116.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 306, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (324.34, 158.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 322, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1070)
[M::mem_pestat] mean and std.dev: (316.16, 128.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1345)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 691120 reads in 169.527 CPU sec, 5.410 real sec
sorting block 53, #reads = 1066938 mem = 536884462 peak = 1038675968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 280965, 10, 57)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 224, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566)
[M::mem_pestat] mean and std.dev: (230.96, 107.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 704)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 314, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (331.10, 161.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (99, 1242, 4923)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14571)
[M::mem_pestat] mean and std.dev: (2250.80, 2432.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 19395)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 336, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (316.90, 140.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 686214 sequences (100000290 bp) in 1.681 CPU sec, 1.682 real sec...
[M::mem_process_seqs] Processed 688582 reads in 185.055 CPU sec, 5.887 real sec
[M::mem_process_seqs] Processed 687418 reads in 203.301 CPU sec, 6.738 real sec
sorting block 54, #reads = 1065062 mem = 536883491 peak = 1038675968
[M::process] read 684004 sequences (100000295 bp) in 2.038 CPU sec, 2.144 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 280339, 8, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 217, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1115)
[M::mem_pestat] mean and std.dev: (287.76, 199.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1433)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 319, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (335.54, 163.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 252, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (266.44, 95.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686824 reads in 95.880 CPU sec, 3.348 real sec
sorting block 55, #reads = 1065045 mem = 536882352 peak = 1038675968
[M::process] read 683760 sequences (100000233 bp) in 2.265 CPU sec, 2.406 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 279281, 8, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 249, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1231)
[M::mem_pestat] mean and std.dev: (269.67, 167.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1597)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 313, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (331.56, 160.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 293, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1065)
[M::mem_pestat] mean and std.dev: (327.78, 176.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1357)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682984 sequences (100000175 bp) in 1.640 CPU sec, 1.647 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 279015, 6, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 254, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (246.73, 105.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 327, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (342.86, 163.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 283, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (287.79, 105.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686214 reads in 190.995 CPU sec, 6.112 real sec
[M::process] read 681856 sequences (100000278 bp) in 1.876 CPU sec, 1.881 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278207, 2, 48)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 303, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (298.25, 129.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 324, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (341.12, 162.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 229, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523)
[M::mem_pestat] mean and std.dev: (222.80, 72.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 637)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683760 reads in 91.334 CPU sec, 2.957 real sec
sorting block 56, #reads = 1064097 mem = 536878395 peak = 1038675968
[M::mem_process_seqs] Processed 684004 reads in 318.568 CPU sec, 10.252 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 278679, 8, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (201, 264, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (265.33, 113.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 737)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 328, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (344.74, 163.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 292, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1136)
[M::mem_pestat] mean and std.dev: (334.97, 218.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1460)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681418 sequences (100000091 bp) in 2.391 CPU sec, 2.595 real sec...
[M::process] read 26380 sequences (3845666 bp) in 0.430 CPU sec, 0.424 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 277787, 3, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (221, 262, 536)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1166)
[M::mem_pestat] mean and std.dev: (342.21, 191.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1481)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 339, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (356.15, 170.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (280, 327, 1253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3199)
[M::mem_pestat] mean and std.dev: (548.51, 526.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4172)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682984 reads in 178.930 CPU sec, 5.754 real sec
sorting block 57, #reads = 1066101 mem = 536881208 peak = 1038675968
[M::mem_process_seqs] Processed 681856 reads in 103.151 CPU sec, 3.311 real sec
sorting block 58, #reads = 1066622 mem = 536876204 peak = 1165824000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 10676, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 324, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (340.58, 166.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 26380 reads in 3.962 CPU sec, 0.137 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 277919, 4, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 237, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (256.48, 112.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 345, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (363.10, 176.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 226, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454)
[M::mem_pestat] mean and std.dev: (218.42, 74.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 550)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681418 reads in 91.933 CPU sec, 3.304 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 63635024
overall: 70031024128 mem 9892.701 user 328.326 sys 759.566 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1029_N5D-T1-TDNA-1_A23MHWWLT4_1.L004\tPU:L004\tSM:1029_N5D-T1-TDNA-1_A23MHWWLT4_1\tLB:1029_N5D-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0001.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0001.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 759.612 sec; CPU: 10221.041 sec
sorting block 59, #reads = 964386 mem = 485584185 peak = 1165824000
uniq header count 1
merging 60 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3968788525
output reads: 64011575
bam_mem_sort: 60 calls 29.239 user 0.365 sys 29.718 real
bam_write: 60 calls 19.966 user 0.601 sys 20.589 real
execute: 1 calls 12.053 user 0.643 sys 782.397 real
merge_files: 1 calls 12.029 user 0.622 sys 21.482 real
parse_chunk: 959 calls 37.876 user 9.572 sys 48.232 real
read_chunk: 2590 calls 21.326 user 17.645 sys 280.329 real
sort_block: 1 calls 15.740 user 16.998 sys 272.084 real
write_chunk: 1629 calls 11.684 user 0.421 sys 20.726 real
overall: 2889023488 mem 829.914 user 35.712 sys 782.418 real
ls: cannot access '*.cram': No such file or directory