.command.log - Workdir - DAQ:SENTIEON_BWAMEM (659_bR4-T1-TDNA-1_A23MHWWLT4_1;L001;0001)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e0/418c4fb582003f8163b3dc53efdae8/.command.log
Size: 253.5 KB
Task: DAQ:SENTIEON_BWAMEM (659_bR4-T1-TDNA-1_A23MHWWLT4_1;L001;0001)
Attempt
Content

  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/fa/a3e8b4686408afc7b4328db5b13bbf/659_bR4-T1-TDNA-1_A23MHWWLT4_1/659_bR4-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e0/418c4fb582003f8163b3dc53efdae8/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/fa/a3e8b4686408afc7b4328db5b13bbf/659_bR4-T1-TDNA-1_A23MHWWLT4_1/659_bR4-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e0/418c4fb582003f8163b3dc53efdae8/.command.run
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_bR4-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1891.335 CPU sec, 77.539 real sec
[M::process] read 676528 sequences (100000269 bp) in 1.686 CPU sec, 1.690 real sec...
[M::process] read 676372 sequences (100000024 bp) in 2.072 CPU sec, 2.181 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 282123, 0, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (548, 1157, 1157)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2375)
[M::mem_pestat] mean and std.dev: (857.17, 309.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2984)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 340, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (353.97, 156.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 676148 sequences (100000086 bp) in 2.092 CPU sec, 2.135 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281441, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 342, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (356.13, 157.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675714 sequences (100000014 bp) in 2.124 CPU sec, 2.137 real sec...
[M::mem_process_seqs] Processed 676528 reads in 191.652 CPU sec, 6.459 real sec
[M::mem_process_seqs] Processed 676372 reads in 126.034 CPU sec, 4.223 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282017, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 345, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (358.36, 157.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 0, #reads = 1079114 mem = 536879192 peak = 575881216
[M::process] read 675058 sequences (100000101 bp) in 2.578 CPU sec, 3.337 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281486, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 350, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (362.74, 159.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675124 sequences (100000269 bp) in 2.085 CPU sec, 2.141 real sec...
[M::mem_process_seqs] Processed 676148 reads in 185.657 CPU sec, 6.110 real sec
[M::mem_process_seqs] Processed 675714 reads in 124.369 CPU sec, 4.071 real sec
sorting block 1, #reads = 1079384 mem = 536877910 peak = 701710336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281280, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 353, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (366.06, 160.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674942 sequences (100000209 bp) in 2.938 CPU sec, 3.804 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 281351, 6, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (1185, 1185, 1185)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1185, 1185)
[M::mem_pestat] mean and std.dev: (1185.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1185, 1185)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 355, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (367.29, 160.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675058 reads in 183.131 CPU sec, 6.141 real sec
[M::mem_process_seqs] Processed 675124 reads in 121.732 CPU sec, 4.094 real sec
[M::process] read 674886 sequences (100000128 bp) in 2.706 CPU sec, 2.972 real sec...
sorting block 2, #reads = 1079815 mem = 536880484 peak = 755621888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281535, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 356, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (369.28, 161.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281562, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 357, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (369.89, 161.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674894 sequences (100000223 bp) in 3.025 CPU sec, 4.079 real sec...
[M::mem_process_seqs] Processed 674942 reads in 182.409 CPU sec, 6.210 real sec
[M::mem_process_seqs] Processed 674886 reads in 123.070 CPU sec, 4.149 real sec
sorting block 3, #reads = 1080190 mem = 536878333 peak = 755621888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280858, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 356, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (368.99, 161.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674532 sequences (100000269 bp) in 3.138 CPU sec, 3.865 real sec...
[M::mem_process_seqs] Processed 674894 reads in 102.958 CPU sec, 3.873 real sec
sorting block 4, #reads = 1080311 mem = 536880504 peak = 759025664
[M::process] read 674480 sequences (100000043 bp) in 2.720 CPU sec, 3.385 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280770, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 358, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (370.40, 160.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281881, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 360, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (372.74, 161.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674352 sequences (100000162 bp) in 2.967 CPU sec, 3.702 real sec...
[M::mem_process_seqs] Processed 674532 reads in 187.934 CPU sec, 6.581 real sec
[M::mem_process_seqs] Processed 674480 reads in 124.323 CPU sec, 4.343 real sec
sorting block 5, #reads = 1079762 mem = 536880034 peak = 759025664
[M::process] read 674172 sequences (100000026 bp) in 3.098 CPU sec, 3.536 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281284, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 362, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (374.95, 162.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280998, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 364, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (376.31, 162.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674352 reads in 192.691 CPU sec, 6.910 real sec
[M::process] read 674332 sequences (100000221 bp) in 3.301 CPU sec, 3.959 real sec...
[M::mem_process_seqs] Processed 674172 reads in 127.470 CPU sec, 4.567 real sec
sorting block 6, #reads = 1079968 mem = 536877021 peak = 789839872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280830, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 364, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (376.87, 164.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675134 sequences (100000216 bp) in 3.028 CPU sec, 3.405 real sec...
[M::mem_process_seqs] Processed 674332 reads in 109.714 CPU sec, 4.015 real sec
sorting block 7, #reads = 1080371 mem = 536879810 peak = 827535360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280439, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 357, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (370.43, 163.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674950 sequences (100000045 bp) in 3.190 CPU sec, 3.717 real sec...
[M::mem_process_seqs] Processed 675134 reads in 110.826 CPU sec, 4.156 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281297, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 355, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (369.09, 162.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674874 sequences (100000129 bp) in 3.354 CPU sec, 4.219 real sec...
[M::mem_process_seqs] Processed 674950 reads in 111.523 CPU sec, 4.110 real sec
sorting block 8, #reads = 1078810 mem = 536878013 peak = 827535360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281512, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 356, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (370.23, 162.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674894 sequences (100000082 bp) in 3.176 CPU sec, 3.873 real sec...
[M::mem_process_seqs] Processed 674874 reads in 110.362 CPU sec, 4.145 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281240, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 357, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (370.31, 162.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674660 sequences (100000123 bp) in 3.014 CPU sec, 3.467 real sec...
sorting block 9, #reads = 1080566 mem = 536877359 peak = 827535360
[M::mem_process_seqs] Processed 674894 reads in 109.797 CPU sec, 4.203 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280809, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 357, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (370.52, 162.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674532 sequences (100000056 bp) in 3.483 CPU sec, 4.195 real sec...
[M::mem_process_seqs] Processed 674660 reads in 111.414 CPU sec, 4.227 real sec
sorting block 10, #reads = 1080789 mem = 536878613 peak = 827535360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281134, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 357, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (371.06, 162.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674696 sequences (100000166 bp) in 3.175 CPU sec, 3.775 real sec...
[M::mem_process_seqs] Processed 674532 reads in 110.634 CPU sec, 4.275 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281005, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 355, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (369.46, 161.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674604 sequences (100000033 bp) in 3.293 CPU sec, 3.870 real sec...
[M::mem_process_seqs] Processed 674696 reads in 111.017 CPU sec, 4.196 real sec
sorting block 11, #reads = 1080530 mem = 536877809 peak = 827535360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281246, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 355, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (369.33, 161.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674264 sequences (100000184 bp) in 3.029 CPU sec, 3.684 real sec...
[M::mem_process_seqs] Processed 674604 reads in 111.087 CPU sec, 4.142 real sec
sorting block 12, #reads = 1080442 mem = 536879654 peak = 827535360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280940, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 355, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (368.78, 160.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674434 sequences (100000145 bp) in 3.169 CPU sec, 3.698 real sec...
[M::mem_process_seqs] Processed 674264 reads in 110.533 CPU sec, 4.165 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280928, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 352, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (365.72, 158.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674194 sequences (100000176 bp) in 3.215 CPU sec, 4.147 real sec...
[M::mem_process_seqs] Processed 674434 reads in 111.627 CPU sec, 4.219 real sec
sorting block 13, #reads = 1080049 mem = 536879545 peak = 827535360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280465, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 351, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (365.77, 158.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674484 sequences (100000241 bp) in 3.265 CPU sec, 3.840 real sec...
[M::mem_process_seqs] Processed 674194 reads in 114.214 CPU sec, 4.091 real sec
sorting block 14, #reads = 1079696 mem = 536880148 peak = 827535360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280233, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 348, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (362.64, 156.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675170 sequences (100000040 bp) in 3.043 CPU sec, 3.282 real sec...
[M::mem_process_seqs] Processed 674484 reads in 111.560 CPU sec, 3.886 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281208, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 342, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (354.19, 151.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1099)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676334 sequences (100000144 bp) in 3.549 CPU sec, 4.018 real sec...
[M::mem_process_seqs] Processed 675170 reads in 116.560 CPU sec, 4.003 real sec
sorting block 15, #reads = 1080376 mem = 536878937 peak = 827535360
[M::process] read 674470 sequences (100000263 bp) in 2.969 CPU sec, 3.507 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280102, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 347, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (361.03, 155.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674470 reads in 105.692 CPU sec, 3.461 real sec
[M::process] read 674624 sequences (100000015 bp) in 3.338 CPU sec, 3.670 real sec...
sorting block 16, #reads = 1078295 mem = 536879816 peak = 827535360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280882, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 346, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (360.03, 155.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674588 sequences (100000121 bp) in 2.974 CPU sec, 3.154 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281956, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 334, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866)
[M::mem_pestat] mean and std.dev: (345.49, 147.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1077)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674624 reads in 107.041 CPU sec, 3.558 real sec
[M::mem_process_seqs] Processed 676334 reads in 334.120 CPU sec, 11.385 real sec
sorting block 17, #reads = 1078832 mem = 536878249 peak = 835588096
[M::process] read 674538 sequences (100000201 bp) in 3.111 CPU sec, 3.301 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280260, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 346, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (361.33, 157.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280450, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 346, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (361.35, 156.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674402 sequences (100000111 bp) in 2.894 CPU sec, 3.090 real sec...
[M::mem_process_seqs] Processed 674588 reads in 185.718 CPU sec, 5.968 real sec
[M::mem_process_seqs] Processed 674538 reads in 123.127 CPU sec, 3.915 real sec
sorting block 18, #reads = 1075158 mem = 536878057 peak = 842948608
[M::process] read 674444 sequences (100000011 bp) in 3.096 CPU sec, 3.278 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280489, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 347, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (361.39, 156.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 19, #reads = 1079437 mem = 536883635 peak = 842948608
[M::process] read 674306 sequences (100000300 bp) in 2.897 CPU sec, 3.029 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280196, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 346, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (360.48, 155.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674402 reads in 182.111 CPU sec, 5.816 real sec
[M::process] read 673980 sequences (100000081 bp) in 2.677 CPU sec, 2.809 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280941, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 346, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (361.21, 155.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674306 reads in 97.356 CPU sec, 3.067 real sec
sorting block 20, #reads = 1079561 mem = 536888047 peak = 842948608
[M::process] read 673994 sequences (100000271 bp) in 2.491 CPU sec, 2.561 real sec...
[M::mem_process_seqs] Processed 674444 reads in 267.778 CPU sec, 8.459 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280604, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 350, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (364.28, 156.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673924 sequences (100000071 bp) in 2.863 CPU sec, 3.030 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281181, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 349, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (364.20, 157.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673980 reads in 189.318 CPU sec, 5.988 real sec
sorting block 21, #reads = 1078887 mem = 536878783 peak = 857616384
[M::mem_process_seqs] Processed 673994 reads in 112.777 CPU sec, 3.560 real sec
[M::process] read 673824 sequences (100000029 bp) in 2.986 CPU sec, 3.111 real sec...
sorting block 22, #reads = 1079895 mem = 536879872 peak = 857616384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281093, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 349, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (363.40, 156.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673722 sequences (100000217 bp) in 2.527 CPU sec, 2.596 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281073, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 349, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (363.83, 156.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673924 reads in 166.368 CPU sec, 5.285 real sec
[M::mem_process_seqs] Processed 673824 reads in 110.690 CPU sec, 3.481 real sec
[M::process] read 673880 sequences (100000199 bp) in 2.799 CPU sec, 2.902 real sec...
sorting block 23, #reads = 1078383 mem = 536879507 peak = 857616384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281345, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 350, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (365.06, 157.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675330 sequences (100000172 bp) in 2.671 CPU sec, 2.785 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281002, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 351, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (365.96, 157.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673722 reads in 163.489 CPU sec, 5.186 real sec
[M::mem_process_seqs] Processed 673880 reads in 108.799 CPU sec, 3.420 real sec
sorting block 24, #reads = 1080051 mem = 536877556 peak = 857616384
[M::process] read 675724 sequences (100000286 bp) in 2.630 CPU sec, 2.700 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281452, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 345, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (358.72, 156.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675596 sequences (100000074 bp) in 2.251 CPU sec, 2.336 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281009, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 346, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (359.73, 158.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675296 sequences (100000105 bp) in 1.665 CPU sec, 1.669 real sec...
[M::mem_process_seqs] Processed 675330 reads in 162.918 CPU sec, 5.161 real sec
sorting block 25, #reads = 1078327 mem = 536878891 peak = 857616384
[M::mem_process_seqs] Processed 675724 reads in 107.396 CPU sec, 3.390 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281123, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 350, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (363.53, 160.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675074 sequences (100000284 bp) in 2.623 CPU sec, 2.719 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280719, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 351, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (364.37, 160.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675596 reads in 157.537 CPU sec, 4.989 real sec
sorting block 26, #reads = 1079656 mem = 536878170 peak = 860008448
[M::process] read 674854 sequences (100000016 bp) in 2.225 CPU sec, 2.250 real sec...
[M::mem_process_seqs] Processed 675296 reads in 103.513 CPU sec, 3.251 real sec
sorting block 27, #reads = 1080995 mem = 536877178 peak = 940470272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281089, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 355, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (368.98, 162.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674854 sequences (100000078 bp) in 2.866 CPU sec, 2.947 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281419, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 358, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (371.68, 163.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675074 reads in 152.244 CPU sec, 4.822 real sec
[M::mem_process_seqs] Processed 674854 reads in 101.418 CPU sec, 3.184 real sec
[M::process] read 674648 sequences (100000119 bp) in 2.453 CPU sec, 2.513 real sec...
sorting block 28, #reads = 1080262 mem = 536878771 peak = 940470272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280946, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 359, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (372.52, 164.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674750 sequences (100000239 bp) in 2.611 CPU sec, 2.700 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281391, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 362, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (375.73, 166.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674854 reads in 145.169 CPU sec, 4.605 real sec
[M::mem_process_seqs] Processed 674648 reads in 96.105 CPU sec, 3.023 real sec
sorting block 29, #reads = 1080567 mem = 536879965 peak = 940470272
[M::process] read 674290 sequences (100000131 bp) in 2.802 CPU sec, 2.885 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281267, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 361, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (373.76, 164.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674304 sequences (100000035 bp) in 2.581 CPU sec, 2.629 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280349, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 362, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (375.01, 163.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674750 reads in 148.113 CPU sec, 4.709 real sec
[M::mem_process_seqs] Processed 674290 reads in 99.430 CPU sec, 3.132 real sec
sorting block 30, #reads = 1081424 mem = 536878134 peak = 940470272
[M::process] read 674076 sequences (100000017 bp) in 2.398 CPU sec, 2.459 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280342, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 363, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (375.90, 164.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 31, #reads = 1080123 mem = 536880961 peak = 940470272
[M::process] read 674106 sequences (100000028 bp) in 2.244 CPU sec, 2.291 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280637, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 365, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (377.30, 164.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674304 reads in 148.314 CPU sec, 4.690 real sec
[M::mem_process_seqs] Processed 674076 reads in 98.988 CPU sec, 3.104 real sec
[M::process] read 674042 sequences (100000261 bp) in 2.474 CPU sec, 2.511 real sec...
sorting block 32, #reads = 1079821 mem = 536882251 peak = 940470272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280831, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 369, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (381.38, 166.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673856 sequences (100000236 bp) in 2.653 CPU sec, 2.712 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280738, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 368, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (380.22, 165.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674106 reads in 148.483 CPU sec, 4.699 real sec
[M::mem_process_seqs] Processed 674042 reads in 98.815 CPU sec, 3.107 real sec
[M::process] read 674618 sequences (100000182 bp) in 2.121 CPU sec, 2.154 real sec...
sorting block 33, #reads = 1080650 mem = 536876484 peak = 940470272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 280204, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 366, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (379.32, 165.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674814 sequences (100000094 bp) in 2.541 CPU sec, 2.601 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280981, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 358, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (372.32, 163.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673856 reads in 150.392 CPU sec, 4.761 real sec
[M::mem_process_seqs] Processed 674618 reads in 100.066 CPU sec, 3.144 real sec
[M::process] read 674488 sequences (100000207 bp) in 2.479 CPU sec, 2.510 real sec...
sorting block 34, #reads = 1079950 mem = 536878670 peak = 940470272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280980, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 357, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (371.65, 163.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674550 sequences (100000276 bp) in 2.637 CPU sec, 2.691 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280952, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 360, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (373.28, 163.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674814 reads in 149.160 CPU sec, 4.719 real sec
[M::mem_process_seqs] Processed 674488 reads in 99.368 CPU sec, 3.121 real sec
sorting block 35, #reads = 1081041 mem = 536878105 peak = 940470272
[M::process] read 674544 sequences (100000203 bp) in 2.532 CPU sec, 2.577 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281125, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 357, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (371.19, 163.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 36, #reads = 1079794 mem = 536884391 peak = 940470272
[M::process] read 674548 sequences (100000088 bp) in 2.611 CPU sec, 2.762 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280470, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 356, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (370.34, 162.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674550 reads in 152.178 CPU sec, 4.836 real sec
[M::mem_process_seqs] Processed 674544 reads in 101.760 CPU sec, 3.209 real sec
[M::process] read 674506 sequences (100000118 bp) in 2.549 CPU sec, 2.581 real sec...
sorting block 37, #reads = 1080341 mem = 536881905 peak = 940470272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280784, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 356, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (370.40, 163.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674610 sequences (100000050 bp) in 2.480 CPU sec, 2.518 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281051, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 353, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (367.89, 161.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674548 reads in 149.562 CPU sec, 4.723 real sec
[M::mem_process_seqs] Processed 674506 reads in 99.754 CPU sec, 3.133 real sec
[M::process] read 674370 sequences (100000272 bp) in 2.436 CPU sec, 2.465 real sec...
sorting block 38, #reads = 1079599 mem = 536879361 peak = 940470272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281448, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 353, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (367.91, 161.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674704 sequences (100000108 bp) in 2.431 CPU sec, 2.485 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281109, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 352, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (367.03, 160.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674610 reads in 147.323 CPU sec, 4.664 real sec
[M::mem_process_seqs] Processed 674370 reads in 97.467 CPU sec, 3.056 real sec
sorting block 39, #reads = 1080362 mem = 536880178 peak = 940470272
[M::process] read 675070 sequences (100000024 bp) in 2.440 CPU sec, 2.466 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280969, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 349, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (364.11, 159.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674978 sequences (100000160 bp) in 2.586 CPU sec, 2.643 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280793, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 348, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (363.61, 160.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674704 reads in 153.063 CPU sec, 4.837 real sec
[M::mem_process_seqs] Processed 675070 reads in 101.973 CPU sec, 3.202 real sec
sorting block 40, #reads = 1080108 mem = 536878494 peak = 940470272
[M::process] read 674988 sequences (100000107 bp) in 2.415 CPU sec, 2.440 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280880, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 347, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (362.85, 159.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 41, #reads = 1079759 mem = 536879077 peak = 940470272
[M::process] read 676048 sequences (100000186 bp) in 2.504 CPU sec, 2.548 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280946, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 346, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (361.37, 158.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674978 reads in 152.807 CPU sec, 4.836 real sec
[M::process] read 674676 sequences (100000054 bp) in 1.921 CPU sec, 1.940 real sec...
[M::mem_process_seqs] Processed 674988 reads in 103.013 CPU sec, 3.236 real sec
sorting block 42, #reads = 1079693 mem = 536886109 peak = 940470272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281339, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 339, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (352.26, 153.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1111)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674724 sequences (100000212 bp) in 2.240 CPU sec, 2.286 real sec...
[M::process] read 674576 sequences (100000166 bp) in 1.620 CPU sec, 1.617 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280877, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 348, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (363.44, 159.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676048 reads in 159.060 CPU sec, 5.043 real sec
[M::mem_process_seqs] Processed 674676 reads in 98.498 CPU sec, 3.125 real sec
sorting block 43, #reads = 1074893 mem = 536878089 peak = 940470272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280908, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 349, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (364.24, 159.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674396 sequences (100000129 bp) in 2.315 CPU sec, 2.373 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281046, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 348, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (364.32, 160.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674388 sequences (100000254 bp) in 1.580 CPU sec, 1.578 real sec...
[M::mem_process_seqs] Processed 674724 reads in 145.151 CPU sec, 4.590 real sec
[M::mem_process_seqs] Processed 674576 reads in 94.621 CPU sec, 2.986 real sec
sorting block 44, #reads = 1079052 mem = 536876695 peak = 940470272
[M::process] read 674320 sequences (100000112 bp) in 2.727 CPU sec, 2.786 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281246, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 349, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (363.87, 158.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674328 sequences (100000225 bp) in 1.600 CPU sec, 1.600 real sec...
[M::mem_process_seqs] Processed 674388 reads in 78.556 CPU sec, 2.462 real sec
sorting block 45, #reads = 1079967 mem = 536879342 peak = 940470272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281387, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 347, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (363.33, 158.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281102, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 349, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (363.90, 158.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674396 reads in 223.385 CPU sec, 7.051 real sec
[M::process] read 674226 sequences (100000017 bp) in 1.692 CPU sec, 1.692 real sec...
[M::mem_process_seqs] Processed 674320 reads in 110.906 CPU sec, 3.518 real sec
sorting block 46, #reads = 1079647 mem = 536877232 peak = 946622464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281433, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 347, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (362.98, 157.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674136 sequences (100000200 bp) in 2.617 CPU sec, 2.688 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281044, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 348, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (363.66, 157.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674328 reads in 142.600 CPU sec, 4.510 real sec
[M::mem_process_seqs] Processed 674226 reads in 95.571 CPU sec, 2.995 real sec
sorting block 47, #reads = 1078772 mem = 536882739 peak = 946622464
[M::process] read 674184 sequences (100000138 bp) in 2.339 CPU sec, 2.377 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281004, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 350, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (364.84, 157.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673726 sequences (100000068 bp) in 2.136 CPU sec, 2.210 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280720, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 352, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (366.20, 159.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674136 reads in 143.290 CPU sec, 4.532 real sec
sorting block 48, #reads = 1078437 mem = 536880191 peak = 946622464
[M::process] read 673826 sequences (100000046 bp) in 2.108 CPU sec, 2.115 real sec...
[M::mem_process_seqs] Processed 674184 reads in 95.203 CPU sec, 2.984 real sec
sorting block 49, #reads = 1079072 mem = 536879369 peak = 1039011840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280537, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 353, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (368.91, 160.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674588 sequences (100000064 bp) in 2.083 CPU sec, 2.150 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280351, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 354, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (370.47, 161.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676174 sequences (100000275 bp) in 1.610 CPU sec, 1.610 real sec...
[M::mem_process_seqs] Processed 673726 reads in 142.368 CPU sec, 4.515 real sec
[M::mem_process_seqs] Processed 673826 reads in 96.466 CPU sec, 3.045 real sec
sorting block 50, #reads = 1077525 mem = 536877975 peak = 1039011840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281661, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 352, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (366.87, 161.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675770 sequences (100000225 bp) in 2.804 CPU sec, 2.862 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281453, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 345, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (359.14, 160.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674588 reads in 145.595 CPU sec, 4.587 real sec
[M::mem_process_seqs] Processed 676174 reads in 96.704 CPU sec, 3.031 real sec
[M::process] read 675560 sequences (100000198 bp) in 2.442 CPU sec, 2.473 real sec...
sorting block 51, #reads = 1078511 mem = 536877493 peak = 1039011840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281836, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 350, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (363.43, 161.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675322 sequences (100000172 bp) in 2.146 CPU sec, 2.223 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281354, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 351, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (365.42, 161.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675056 sequences (100000097 bp) in 1.590 CPU sec, 1.593 real sec...
[M::mem_process_seqs] Processed 675770 reads in 143.861 CPU sec, 4.555 real sec
sorting block 52, #reads = 1079038 mem = 536881709 peak = 1039011840
[M::mem_process_seqs] Processed 675560 reads in 96.354 CPU sec, 3.040 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281568, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 355, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (368.83, 163.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674530 sequences (100000138 bp) in 2.779 CPU sec, 2.840 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281862, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 355, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (368.91, 162.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675322 reads in 140.767 CPU sec, 4.444 real sec
sorting block 53, #reads = 1080399 mem = 536880102 peak = 1039011840
[M::mem_process_seqs] Processed 675056 reads in 94.589 CPU sec, 2.969 real sec
[M::process] read 674442 sequences (100000003 bp) in 2.658 CPU sec, 2.770 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282095, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 358, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (371.49, 162.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 54, #reads = 1079792 mem = 536878994 peak = 1039011840
[M::process] read 674440 sequences (100000272 bp) in 2.110 CPU sec, 2.182 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281476, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 359, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (373.07, 163.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674530 reads in 143.643 CPU sec, 4.565 real sec
[M::mem_process_seqs] Processed 674442 reads in 97.358 CPU sec, 3.062 real sec
[M::process] read 674222 sequences (100000242 bp) in 1.887 CPU sec, 1.906 real sec...
sorting block 55, #reads = 1080030 mem = 536877810 peak = 1039011840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280815, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 362, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (374.60, 163.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673780 sequences (100000128 bp) in 2.818 CPU sec, 2.901 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281201, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 365, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (377.77, 164.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674440 reads in 141.295 CPU sec, 4.473 real sec
[M::mem_process_seqs] Processed 674222 reads in 94.545 CPU sec, 2.969 real sec
sorting block 56, #reads = 1079720 mem = 536880430 peak = 1039011840
[M::process] read 673782 sequences (100000116 bp) in 2.324 CPU sec, 2.391 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281348, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 366, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (379.19, 164.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673638 sequences (100000220 bp) in 2.402 CPU sec, 2.465 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280745, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 367, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (380.87, 166.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673780 reads in 143.051 CPU sec, 4.528 real sec
[M::mem_process_seqs] Processed 673782 reads in 96.177 CPU sec, 3.026 real sec
sorting block 57, #reads = 1079277 mem = 536879958 peak = 1039011840
[M::process] read 673724 sequences (100000122 bp) in 2.366 CPU sec, 2.387 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281827, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 371, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (384.38, 167.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673638 sequences (100000153 bp) in 2.645 CPU sec, 2.713 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281373, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 371, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (384.62, 168.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673638 reads in 141.350 CPU sec, 4.473 real sec
sorting block 58, #reads = 1078923 mem = 536879216 peak = 1039011840
[M::mem_process_seqs] Processed 673724 reads in 94.444 CPU sec, 2.974 real sec
[M::process] read 674362 sequences (100000057 bp) in 2.525 CPU sec, 2.570 real sec...
sorting block 59, #reads = 1080431 mem = 536878050 peak = 1039011840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280980, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 372, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (384.70, 167.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674794 sequences (100000297 bp) in 2.502 CPU sec, 2.552 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281093, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 361, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (375.82, 165.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673638 reads in 145.511 CPU sec, 4.601 real sec
[M::mem_process_seqs] Processed 674362 reads in 96.904 CPU sec, 3.039 real sec
[M::process] read 674740 sequences (100000287 bp) in 2.541 CPU sec, 2.587 real sec...
sorting block 60, #reads = 1078710 mem = 536880059 peak = 1039011840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281172, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 356, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (371.39, 164.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674408 sequences (100000093 bp) in 2.561 CPU sec, 2.626 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281601, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 355, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (369.91, 163.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674794 reads in 142.621 CPU sec, 4.519 real sec
[M::mem_process_seqs] Processed 674740 reads in 94.776 CPU sec, 2.978 real sec
sorting block 61, #reads = 1079157 mem = 536880379 peak = 1039011840
[M::process] read 674426 sequences (100000122 bp) in 2.594 CPU sec, 2.634 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281205, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 359, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (373.12, 164.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674252 sequences (100000297 bp) in 2.126 CPU sec, 2.168 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281474, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 357, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (372.14, 163.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674408 reads in 140.250 CPU sec, 4.433 real sec
sorting block 62, #reads = 1079583 mem = 536878742 peak = 1039011840
[M::process] read 674284 sequences (100000149 bp) in 2.024 CPU sec, 2.066 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281167, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 356, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (371.29, 164.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674418 sequences (100000163 bp) in 1.559 CPU sec, 1.568 real sec...
[M::mem_process_seqs] Processed 674252 reads in 81.397 CPU sec, 2.570 real sec
sorting block 63, #reads = 1080781 mem = 536878489 peak = 1039011840
[M::mem_process_seqs] Processed 674426 reads in 198.260 CPU sec, 6.252 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280526, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 354, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (370.53, 163.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674534 sequences (100000205 bp) in 2.050 CPU sec, 2.131 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281086, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 354, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (370.28, 163.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674472 sequences (100000137 bp) in 1.590 CPU sec, 1.581 real sec...
[M::mem_process_seqs] Processed 674284 reads in 160.774 CPU sec, 5.082 real sec
sorting block 64, #reads = 1079373 mem = 536879361 peak = 1039011840
[M::mem_process_seqs] Processed 674418 reads in 96.047 CPU sec, 3.030 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281623, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 354, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (369.57, 163.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674826 sequences (100000272 bp) in 2.720 CPU sec, 2.771 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281430, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 352, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (367.79, 162.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674534 reads in 142.456 CPU sec, 4.494 real sec
sorting block 65, #reads = 1079723 mem = 536881605 peak = 1039011840
[M::mem_process_seqs] Processed 674472 reads in 95.007 CPU sec, 2.975 real sec
[M::process] read 674560 sequences (100000047 bp) in 2.319 CPU sec, 2.343 real sec...
sorting block 66, #reads = 1079398 mem = 536880270 peak = 1042104320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281142, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 350, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (366.81, 162.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674972 sequences (100000158 bp) in 2.286 CPU sec, 2.336 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280780, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 351, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (367.03, 162.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674826 reads in 144.788 CPU sec, 4.587 real sec
[M::process] read 674834 sequences (100000069 bp) in 1.685 CPU sec, 1.690 real sec...
[M::mem_process_seqs] Processed 674560 reads in 96.969 CPU sec, 3.049 real sec
sorting block 67, #reads = 1079297 mem = 536879622 peak = 1042104320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280868, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 346, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (361.95, 159.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674684 sequences (100000116 bp) in 2.768 CPU sec, 2.822 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281399, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 347, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (361.84, 158.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674972 reads in 147.672 CPU sec, 4.662 real sec
[M::mem_process_seqs] Processed 674834 reads in 98.867 CPU sec, 3.097 real sec
[M::process] read 674270 sequences (100000109 bp) in 2.335 CPU sec, 2.354 real sec...
sorting block 68, #reads = 1079507 mem = 536881248 peak = 1042104320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281614, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 350, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (365.50, 161.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674484 sequences (100000016 bp) in 2.452 CPU sec, 2.506 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281196, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 352, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (368.30, 162.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674684 reads in 145.554 CPU sec, 4.607 real sec
[M::mem_process_seqs] Processed 674270 reads in 96.584 CPU sec, 3.027 real sec
sorting block 69, #reads = 1077876 mem = 536879718 peak = 1042104320
[M::process] read 674368 sequences (100000230 bp) in 2.160 CPU sec, 2.173 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281370, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 353, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (369.23, 163.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674196 sequences (100000085 bp) in 2.538 CPU sec, 2.611 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281075, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 354, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (369.65, 163.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674484 reads in 145.380 CPU sec, 4.593 real sec
sorting block 70, #reads = 1079086 mem = 536887186 peak = 1042104320
[M::mem_process_seqs] Processed 674368 reads in 94.695 CPU sec, 2.970 real sec
[M::process] read 674196 sequences (100000295 bp) in 2.374 CPU sec, 2.389 real sec...
sorting block 71, #reads = 1080316 mem = 536879231 peak = 1051832320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280669, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 351, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (367.74, 162.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674154 sequences (100000189 bp) in 2.492 CPU sec, 2.549 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281129, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 353, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (369.22, 162.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674196 reads in 140.164 CPU sec, 4.439 real sec
[M::mem_process_seqs] Processed 674196 reads in 92.070 CPU sec, 2.892 real sec
[M::process] read 674030 sequences (100000169 bp) in 2.371 CPU sec, 2.412 real sec...
sorting block 72, #reads = 1080067 mem = 536879204 peak = 1051832320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280726, 10, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 353, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (369.28, 162.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3776, 6859, 6859)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13025)
[M::mem_pestat] mean and std.dev: (5194.10, 2716.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16108)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 231, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (231, 231)
[M::mem_pestat] mean and std.dev: (231.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (231, 231)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 674128 sequences (100000177 bp) in 2.587 CPU sec, 2.643 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 281256, 1, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (365, 365, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (365, 365)
[M::mem_pestat] mean and std.dev: (365.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (365, 365)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 353, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (369.45, 162.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 674154 reads in 142.729 CPU sec, 4.513 real sec
[M::mem_process_seqs] Processed 674030 reads in 96.162 CPU sec, 3.012 real sec
[M::process] read 674066 sequences (100000145 bp) in 2.152 CPU sec, 2.190 real sec...
sorting block 73, #reads = 1079828 mem = 536876890 peak = 1051832320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280590, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 353, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (369.06, 161.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674084 sequences (100000149 bp) in 1.968 CPU sec, 2.019 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281267, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 354, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (370.35, 162.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673822 sequences (100000221 bp) in 1.500 CPU sec, 1.506 real sec...
[M::mem_process_seqs] Processed 674128 reads in 141.622 CPU sec, 4.491 real sec
sorting block 74, #reads = 1079937 mem = 536878332 peak = 1051832320
[M::mem_process_seqs] Processed 674066 reads in 94.150 CPU sec, 2.971 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281185, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 354, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (370.32, 163.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673640 sequences (100000233 bp) in 2.703 CPU sec, 2.774 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280808, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 357, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (373.22, 164.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674084 reads in 143.544 CPU sec, 4.523 real sec
sorting block 75, #reads = 1079838 mem = 536878657 peak = 1054990336
[M::mem_process_seqs] Processed 673822 reads in 93.752 CPU sec, 2.941 real sec
[M::process] read 675118 sequences (100000065 bp) in 2.381 CPU sec, 2.402 real sec...
sorting block 76, #reads = 1078959 mem = 536880399 peak = 1074180096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280915, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 357, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (372.43, 163.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675604 sequences (100000065 bp) in 2.095 CPU sec, 2.143 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281684, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 347, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (361.58, 158.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675268 sequences (100000218 bp) in 1.590 CPU sec, 1.596 real sec...
[M::mem_process_seqs] Processed 673640 reads in 143.612 CPU sec, 4.547 real sec
[M::mem_process_seqs] Processed 675118 reads in 95.195 CPU sec, 3.002 real sec
sorting block 77, #reads = 1078742 mem = 536879020 peak = 1074180096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280986, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 349, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (363.89, 162.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675004 sequences (100000273 bp) in 2.849 CPU sec, 2.921 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280657, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 353, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (367.20, 163.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675604 reads in 142.062 CPU sec, 4.489 real sec
[M::mem_process_seqs] Processed 675268 reads in 93.652 CPU sec, 2.936 real sec
[M::process] read 674658 sequences (100000074 bp) in 2.456 CPU sec, 2.491 real sec...
sorting block 78, #reads = 1080663 mem = 536877413 peak = 1074180096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281135, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 355, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (370.16, 164.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674642 sequences (100000252 bp) in 2.396 CPU sec, 2.449 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280637, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 357, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (371.89, 164.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675004 reads in 140.745 CPU sec, 4.455 real sec
[M::mem_process_seqs] Processed 674658 reads in 93.683 CPU sec, 2.938 real sec
sorting block 79, #reads = 1080658 mem = 536880664 peak = 1074180096
[M::process] read 674370 sequences (100000033 bp) in 2.497 CPU sec, 2.526 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280635, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 360, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (374.41, 165.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674422 sequences (100000293 bp) in 2.163 CPU sec, 2.213 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281280, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 362, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (376.75, 166.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674642 reads in 140.991 CPU sec, 4.463 real sec
sorting block 80, #reads = 1081607 mem = 536877709 peak = 1074180096
[M::process] read 674128 sequences (100000109 bp) in 1.623 CPU sec, 1.630 real sec...
[M::mem_process_seqs] Processed 674370 reads in 93.539 CPU sec, 2.940 real sec
sorting block 81, #reads = 1080956 mem = 536879343 peak = 1098829824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280513, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 362, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (377.28, 167.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674144 sequences (100000066 bp) in 2.543 CPU sec, 2.607 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280750, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 364, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (378.54, 166.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674422 reads in 143.355 CPU sec, 4.535 real sec
[M::mem_process_seqs] Processed 674128 reads in 96.106 CPU sec, 3.014 real sec
[M::process] read 673858 sequences (100000299 bp) in 2.342 CPU sec, 2.354 real sec...
sorting block 82, #reads = 1080656 mem = 536880565 peak = 1098829824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280971, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 363, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (377.89, 167.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673630 sequences (100000227 bp) in 2.552 CPU sec, 2.604 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280756, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 367, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (379.86, 165.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674144 reads in 141.943 CPU sec, 4.482 real sec
[M::mem_process_seqs] Processed 673858 reads in 94.665 CPU sec, 2.970 real sec
[M::process] read 673542 sequences (100000031 bp) in 2.392 CPU sec, 2.413 real sec...
sorting block 83, #reads = 1081014 mem = 536877266 peak = 1098829824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 279974, 12, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 368, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (382.10, 167.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (954, 954, 954)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (954, 954)
[M::mem_pestat] mean and std.dev: (954.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (954, 954)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673274 sequences (100000059 bp) in 2.427 CPU sec, 2.487 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280999, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 369, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (382.85, 166.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673630 reads in 141.789 CPU sec, 4.489 real sec
[M::mem_process_seqs] Processed 673542 reads in 95.114 CPU sec, 2.983 real sec
sorting block 84, #reads = 1080907 mem = 536881075 peak = 1098829824
[M::process] read 673374 sequences (100000140 bp) in 2.461 CPU sec, 2.487 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 280637, 0, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (599, 599, 599)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (599, 599)
[M::mem_pestat] mean and std.dev: (599.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (599, 599)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 372, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (384.48, 167.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674120 sequences (100000166 bp) in 2.494 CPU sec, 2.544 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281014, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 373, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (386.82, 168.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673274 reads in 142.162 CPU sec, 4.497 real sec
sorting block 85, #reads = 1079768 mem = 536879753 peak = 1098829824
[M::mem_process_seqs] Processed 673374 reads in 95.774 CPU sec, 3.001 real sec
[M::process] read 674538 sequences (100000146 bp) in 2.499 CPU sec, 2.532 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280995, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 366, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (380.00, 167.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 86, #reads = 1079559 mem = 536880456 peak = 1098829824
[M::process] read 674712 sequences (100000215 bp) in 2.804 CPU sec, 2.962 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281134, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 361, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (375.56, 166.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674120 reads in 145.193 CPU sec, 4.605 real sec
[M::mem_process_seqs] Processed 674538 reads in 96.178 CPU sec, 3.029 real sec
[M::process] read 674248 sequences (100000185 bp) in 1.975 CPU sec, 2.026 real sec...
sorting block 87, #reads = 1080782 mem = 536880316 peak = 1098829824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281098, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 357, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (373.09, 166.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674366 sequences (100000247 bp) in 2.276 CPU sec, 2.332 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281477, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 362, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (376.18, 166.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (608, 608, 608)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (608, 608)
[M::mem_pestat] mean and std.dev: (608.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (608, 608)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 674712 reads in 146.530 CPU sec, 4.634 real sec
[M::mem_process_seqs] Processed 674248 reads in 97.510 CPU sec, 3.056 real sec
[M::process] read 674288 sequences (100000105 bp) in 2.090 CPU sec, 2.103 real sec...
sorting block 88, #reads = 1080577 mem = 536879404 peak = 1098829824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281223, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 358, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (373.55, 164.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674412 sequences (100000175 bp) in 2.466 CPU sec, 2.512 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281248, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 356, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (371.44, 163.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674366 reads in 143.361 CPU sec, 4.536 real sec
[M::mem_process_seqs] Processed 674288 reads in 94.326 CPU sec, 2.954 real sec
sorting block 89, #reads = 1080465 mem = 536877846 peak = 1098829824
[M::process] read 674482 sequences (100000066 bp) in 2.344 CPU sec, 2.363 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280873, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 355, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (370.40, 163.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674692 sequences (100000240 bp) in 2.158 CPU sec, 2.204 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281070, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 354, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (369.06, 162.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674872 sequences (100000098 bp) in 1.520 CPU sec, 1.523 real sec...
[M::mem_process_seqs] Processed 674412 reads in 145.744 CPU sec, 4.613 real sec
sorting block 90, #reads = 1080534 mem = 536881037 peak = 1098829824
[M::process] read 674530 sequences (100000294 bp) in 1.656 CPU sec, 1.657 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280908, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 352, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (367.46, 162.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674692 reads in 84.822 CPU sec, 2.688 real sec
sorting block 91, #reads = 1080208 mem = 536878957 peak = 1109147648
[M::mem_process_seqs] Processed 674482 reads in 196.406 CPU sec, 6.224 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280917, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 351, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (365.67, 160.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674476 sequences (100000223 bp) in 2.364 CPU sec, 2.434 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280909, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 352, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (366.62, 160.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674482 sequences (100000205 bp) in 1.570 CPU sec, 1.577 real sec...
[M::mem_process_seqs] Processed 674872 reads in 164.323 CPU sec, 5.204 real sec
sorting block 92, #reads = 1080045 mem = 536881955 peak = 1109147648
[M::mem_process_seqs] Processed 674530 reads in 98.657 CPU sec, 3.117 real sec
sorting block 93, #reads = 1080291 mem = 536878056 peak = 1109147648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280788, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 351, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (366.22, 160.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674380 sequences (100000290 bp) in 2.758 CPU sec, 2.834 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281074, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 352, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (366.63, 160.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674476 reads in 147.786 CPU sec, 4.649 real sec
[M::process] read 674222 sequences (100000288 bp) in 2.262 CPU sec, 2.273 real sec...
[M::mem_process_seqs] Processed 674482 reads in 99.006 CPU sec, 3.106 real sec
sorting block 94, #reads = 1078760 mem = 536879457 peak = 1109147648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 280748, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 351, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (366.13, 160.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674378 sequences (100000058 bp) in 2.657 CPU sec, 2.716 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280148, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 352, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (367.92, 161.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674380 reads in 146.281 CPU sec, 4.612 real sec
[M::mem_process_seqs] Processed 674222 reads in 98.597 CPU sec, 3.091 real sec
[M::process] read 674348 sequences (100000227 bp) in 2.291 CPU sec, 2.296 real sec...
sorting block 95, #reads = 1078902 mem = 536877985 peak = 1109147648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280560, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 353, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (368.65, 162.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674400 sequences (100000106 bp) in 2.682 CPU sec, 2.737 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280583, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 353, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (369.12, 162.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674378 reads in 148.834 CPU sec, 4.703 real sec
[M::mem_process_seqs] Processed 674348 reads in 98.552 CPU sec, 3.086 real sec
[M::process] read 674458 sequences (100000207 bp) in 2.348 CPU sec, 2.364 real sec...
sorting block 96, #reads = 1079688 mem = 536880565 peak = 1109147648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280970, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 352, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (368.26, 162.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674142 sequences (100000169 bp) in 2.614 CPU sec, 2.759 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280407, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 353, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (368.87, 162.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674400 reads in 150.272 CPU sec, 4.759 real sec
sorting block 97, #reads = 1078424 mem = 536877957 peak = 1109147648
[M::mem_process_seqs] Processed 674458 reads in 100.765 CPU sec, 3.173 real sec
[M::process] read 674100 sequences (100000054 bp) in 2.379 CPU sec, 2.399 real sec...
sorting block 98, #reads = 1079291 mem = 536878373 peak = 1109147648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281181, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 351, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (366.52, 159.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673906 sequences (100000266 bp) in 2.664 CPU sec, 2.720 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281402, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 354, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (369.02, 160.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674142 reads in 149.213 CPU sec, 4.717 real sec
[M::mem_process_seqs] Processed 674100 reads in 98.607 CPU sec, 3.096 real sec
[M::process] read 673860 sequences (100000004 bp) in 2.372 CPU sec, 2.387 real sec...
sorting block 99, #reads = 1078828 mem = 536878574 peak = 1109147648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280550, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 356, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (369.53, 160.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673734 sequences (100000081 bp) in 2.637 CPU sec, 2.686 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280875, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 355, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (369.71, 160.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673906 reads in 144.422 CPU sec, 4.567 real sec
[M::mem_process_seqs] Processed 673860 reads in 96.652 CPU sec, 3.032 real sec
[M::process] read 673820 sequences (100000035 bp) in 2.261 CPU sec, 2.307 real sec...
sorting block 100, #reads = 1078423 mem = 536879915 peak = 1109147648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280253, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 357, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (371.37, 160.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673622 sequences (100000046 bp) in 2.894 CPU sec, 3.042 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281387, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 356, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (371.04, 161.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673734 reads in 146.582 CPU sec, 4.650 real sec
[M::mem_process_seqs] Processed 673820 reads in 97.440 CPU sec, 3.070 real sec
sorting block 101, #reads = 1078736 mem = 536878883 peak = 1109147648
[M::process] read 673370 sequences (100000284 bp) in 2.636 CPU sec, 2.684 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281490, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 357, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (371.36, 161.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675834 sequences (100000203 bp) in 2.553 CPU sec, 2.586 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280957, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 360, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (374.74, 162.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673622 reads in 140.940 CPU sec, 4.466 real sec
sorting block 102, #reads = 1079122 mem = 536881339 peak = 1109147648
[M::mem_process_seqs] Processed 673370 reads in 91.301 CPU sec, 2.868 real sec
[M::process] read 679042 sequences (100000074 bp) in 2.802 CPU sec, 2.847 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281913, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 347, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (361.92, 161.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 103, #reads = 1080140 mem = 536878760 peak = 1109147648
[M::process] read 678464 sequences (100000189 bp) in 2.445 CPU sec, 2.476 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282087, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 327, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (345.71, 160.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675834 reads in 155.531 CPU sec, 4.923 real sec
[M::mem_process_seqs] Processed 679042 reads in 104.507 CPU sec, 3.279 real sec
sorting block 104, #reads = 1076942 mem = 536879139 peak = 1109147648
[M::process] read 678250 sequences (100000228 bp) in 2.721 CPU sec, 2.752 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282693, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 332, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (349.89, 161.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678182 sequences (100000024 bp) in 2.457 CPU sec, 2.489 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282259, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 334, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (352.04, 162.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678464 reads in 156.499 CPU sec, 4.939 real sec
[M::mem_process_seqs] Processed 678250 reads in 102.769 CPU sec, 3.225 real sec
sorting block 105, #reads = 1078313 mem = 536879743 peak = 1109147648
[M::process] read 678280 sequences (100000206 bp) in 2.620 CPU sec, 2.665 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282707, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 334, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (350.85, 160.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677816 sequences (100000067 bp) in 2.579 CPU sec, 2.613 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282508, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 332, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (348.66, 158.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678182 reads in 160.184 CPU sec, 5.064 real sec
[M::mem_process_seqs] Processed 678280 reads in 105.503 CPU sec, 3.312 real sec
sorting block 106, #reads = 1078200 mem = 536880758 peak = 1109147648
[M::process] read 677276 sequences (100000064 bp) in 2.371 CPU sec, 2.385 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282689, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 337, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (352.35, 158.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676642 sequences (100000168 bp) in 2.665 CPU sec, 2.713 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281891, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 341, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (355.01, 159.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677816 reads in 163.841 CPU sec, 5.168 real sec
[M::process] read 676250 sequences (100000076 bp) in 1.949 CPU sec, 1.947 real sec...
sorting block 107, #reads = 1076110 mem = 536881278 peak = 1109147648
[M::mem_process_seqs] Processed 677276 reads in 106.771 CPU sec, 3.358 real sec
sorting block 108, #reads = 1076223 mem = 536881732 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281767, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 347, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (359.55, 159.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676332 sequences (100000274 bp) in 2.576 CPU sec, 2.644 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281992, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 350, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (362.35, 160.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676642 reads in 158.264 CPU sec, 4.984 real sec
[M::process] read 676310 sequences (100000067 bp) in 2.249 CPU sec, 2.251 real sec...
[M::mem_process_seqs] Processed 676250 reads in 103.312 CPU sec, 3.243 real sec
sorting block 109, #reads = 1076352 mem = 536877662 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281464, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 349, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (361.04, 160.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676898 sequences (100000235 bp) in 2.603 CPU sec, 2.636 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281459, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 349, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (362.24, 161.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676332 reads in 153.014 CPU sec, 4.822 real sec
[M::process] read 677724 sequences (100000283 bp) in 2.362 CPU sec, 2.373 real sec...
[M::mem_process_seqs] Processed 676310 reads in 100.219 CPU sec, 3.143 real sec
sorting block 110, #reads = 1076863 mem = 536879566 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282413, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 343, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (355.79, 158.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678048 sequences (100000157 bp) in 2.630 CPU sec, 2.662 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282253, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 335, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (349.98, 157.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676898 reads in 155.879 CPU sec, 4.933 real sec
[M::mem_process_seqs] Processed 677724 reads in 103.981 CPU sec, 3.258 real sec
sorting block 111, #reads = 1077128 mem = 536878833 peak = 1164419072
[M::process] read 678064 sequences (100000095 bp) in 2.483 CPU sec, 2.500 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282836, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 333, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (348.52, 157.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 5010, 5010)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14486)
[M::mem_pestat] mean and std.dev: (3575.80, 2191.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 19224)
[M::mem_pestat] skip orientation RR
[M::process] read 677864 sequences (100000197 bp) in 2.686 CPU sec, 2.730 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282191, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 333, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (348.34, 156.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677774 sequences (100000278 bp) in 1.690 CPU sec, 1.695 real sec...
[M::mem_process_seqs] Processed 678048 reads in 167.111 CPU sec, 5.284 real sec
sorting block 112, #reads = 1076815 mem = 536880899 peak = 1164419072
[M::mem_process_seqs] Processed 678064 reads in 111.788 CPU sec, 3.523 real sec
sorting block 113, #reads = 1074926 mem = 536879321 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282130, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 334, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (349.58, 157.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677594 sequences (100000153 bp) in 2.740 CPU sec, 2.796 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 282893, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 335, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (349.86, 156.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676854 sequences (100000265 bp) in 2.270 CPU sec, 2.271 real sec...
[M::mem_process_seqs] Processed 677864 reads in 174.436 CPU sec, 5.502 real sec
[M::mem_process_seqs] Processed 677774 reads in 114.313 CPU sec, 3.605 real sec
sorting block 114, #reads = 1074490 mem = 536877811 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282285, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 336, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (349.93, 155.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676226 sequences (100000037 bp) in 2.806 CPU sec, 2.859 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281886, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 341, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (354.55, 157.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676366 sequences (100000158 bp) in 2.290 CPU sec, 2.287 real sec...
[M::mem_process_seqs] Processed 677594 reads in 172.101 CPU sec, 5.416 real sec
[M::mem_process_seqs] Processed 676854 reads in 190.223 CPU sec, 6.012 real sec
sorting block 115, #reads = 1072935 mem = 536877893 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281994, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 346, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (359.58, 158.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676102 sequences (100000244 bp) in 2.678 CPU sec, 2.746 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 281730, 1, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (238, 875, 875)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2149)
[M::mem_pestat] mean and std.dev: (566.33, 347.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2786)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 342, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (355.43, 156.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 676226 reads in 162.737 CPU sec, 5.140 real sec
sorting block 116, #reads = 1075082 mem = 536875900 peak = 1164419072
[M::mem_process_seqs] Processed 676366 reads in 105.543 CPU sec, 3.312 real sec
[M::process] read 679304 sequences (100000272 bp) in 2.525 CPU sec, 2.522 real sec...
sorting block 117, #reads = 1075701 mem = 536880965 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281579, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 346, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (360.11, 158.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676850 sequences (100000224 bp) in 2.918 CPU sec, 2.980 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 283421, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 322, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (334.74, 147.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1094)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676644 sequences (100000020 bp) in 2.290 CPU sec, 2.294 real sec...
[M::mem_process_seqs] Processed 676102 reads in 177.734 CPU sec, 5.599 real sec
[M::mem_process_seqs] Processed 679304 reads in 127.199 CPU sec, 4.004 real sec
sorting block 118, #reads = 1073676 mem = 536878871 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281907, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 337, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (350.07, 153.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676228 sequences (100000289 bp) in 2.760 CPU sec, 2.814 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281947, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 336, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (349.40, 152.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675794 sequences (100000063 bp) in 2.310 CPU sec, 2.305 real sec...
[M::mem_process_seqs] Processed 676850 reads in 182.353 CPU sec, 5.754 real sec
[M::mem_process_seqs] Processed 676644 reads in 119.105 CPU sec, 3.760 real sec
sorting block 119, #reads = 1068596 mem = 536879826 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281680, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 339, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (352.73, 154.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675952 sequences (100000054 bp) in 3.150 CPU sec, 3.256 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281621, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 344, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (357.51, 156.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676228 reads in 174.866 CPU sec, 5.509 real sec
[M::process] read 675740 sequences (100000206 bp) in 2.348 CPU sec, 2.346 real sec...
[M::mem_process_seqs] Processed 675794 reads in 114.529 CPU sec, 3.592 real sec
sorting block 120, #reads = 1070449 mem = 536877650 peak = 1164419072
[M::process] read 675438 sequences (100000283 bp) in 2.677 CPU sec, 2.733 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281938, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 347, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (359.65, 157.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675924 sequences (100000038 bp) in 2.320 CPU sec, 2.322 real sec...
[M::mem_process_seqs] Processed 675740 reads in 101.124 CPU sec, 3.169 real sec
sorting block 121, #reads = 1073034 mem = 536876909 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282026, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 343, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (356.86, 156.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281165, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 348, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (360.99, 158.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675872 sequences (100000077 bp) in 2.415 CPU sec, 2.423 real sec...
[M::mem_process_seqs] Processed 675952 reads in 274.102 CPU sec, 8.642 real sec
[M::mem_process_seqs] Processed 675438 reads in 138.807 CPU sec, 4.390 real sec
sorting block 122, #reads = 1073398 mem = 536881603 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282014, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 345, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (359.27, 158.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675434 sequences (100000088 bp) in 3.063 CPU sec, 3.170 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281845, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 342, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (356.78, 157.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677084 sequences (100000197 bp) in 2.309 CPU sec, 2.312 real sec...
[M::mem_process_seqs] Processed 675924 reads in 191.934 CPU sec, 6.058 real sec
sorting block 123, #reads = 1072060 mem = 536880117 peak = 1164419072
[M::mem_process_seqs] Processed 675872 reads in 133.292 CPU sec, 4.209 real sec
sorting block 124, #reads = 1070787 mem = 536882029 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281623, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 348, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (362.59, 160.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683018 sequences (100000071 bp) in 2.911 CPU sec, 2.995 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282504, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 338, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (355.22, 161.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681080 sequences (100000040 bp) in 2.380 CPU sec, 2.382 real sec...
[M::mem_process_seqs] Processed 675434 reads in 196.441 CPU sec, 6.200 real sec
[M::mem_process_seqs] Processed 677084 reads in 130.771 CPU sec, 4.137 real sec
sorting block 125, #reads = 1072276 mem = 536878784 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284454, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 301, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (319.80, 148.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1078)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679192 sequences (100000270 bp) in 3.000 CPU sec, 3.049 real sec...
[M::process] read 679092 sequences (100000261 bp) in 2.370 CPU sec, 2.377 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283387, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 312, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881)
[M::mem_pestat] mean and std.dev: (329.34, 151.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1105)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683018 reads in 209.413 CPU sec, 6.618 real sec
[M::mem_process_seqs] Processed 681080 reads in 137.813 CPU sec, 4.365 real sec
sorting block 126, #reads = 1068774 mem = 536879905 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283386, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 323, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (338.89, 154.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678974 sequences (100000238 bp) in 3.138 CPU sec, 3.213 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282758, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 324, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (340.35, 154.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678340 sequences (100000187 bp) in 2.370 CPU sec, 2.370 real sec...
[M::mem_process_seqs] Processed 679192 reads in 191.779 CPU sec, 6.050 real sec
sorting block 127, #reads = 1071902 mem = 536879835 peak = 1164419072
[M::mem_process_seqs] Processed 679092 reads in 125.903 CPU sec, 3.975 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283322, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 325, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (340.45, 153.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677564 sequences (100000242 bp) in 2.977 CPU sec, 3.048 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282955, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 332, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (345.13, 154.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677632 sequences (100000278 bp) in 2.410 CPU sec, 2.409 real sec...
[M::mem_process_seqs] Processed 678974 reads in 196.234 CPU sec, 6.196 real sec
sorting block 128, #reads = 1073871 mem = 536879967 peak = 1164419072
[M::mem_process_seqs] Processed 678340 reads in 127.862 CPU sec, 4.049 real sec
sorting block 129, #reads = 1071878 mem = 536879958 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282793, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 336, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (348.38, 154.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676946 sequences (100000298 bp) in 3.098 CPU sec, 3.179 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282660, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 336, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (348.95, 154.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676746 sequences (100000243 bp) in 2.353 CPU sec, 2.358 real sec...
[M::mem_process_seqs] Processed 677564 reads in 190.977 CPU sec, 6.025 real sec
[M::mem_process_seqs] Processed 677632 reads in 126.362 CPU sec, 3.983 real sec
sorting block 130, #reads = 1072580 mem = 536879413 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282367, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 340, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (352.56, 155.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677800 sequences (100000049 bp) in 3.018 CPU sec, 3.077 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282027, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 342, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (355.50, 157.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677576 sequences (100000262 bp) in 2.370 CPU sec, 2.367 real sec...
[M::mem_process_seqs] Processed 676946 reads in 186.022 CPU sec, 5.864 real sec
[M::mem_process_seqs] Processed 676746 reads in 122.272 CPU sec, 3.860 real sec
sorting block 131, #reads = 1073759 mem = 536878334 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282490, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 333, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (348.46, 156.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677566 sequences (100000001 bp) in 3.144 CPU sec, 3.241 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282731, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 333, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (348.07, 156.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677800 reads in 180.169 CPU sec, 5.683 real sec
[M::process] read 677578 sequences (100000203 bp) in 2.398 CPU sec, 2.393 real sec...
[M::mem_process_seqs] Processed 677576 reads in 117.808 CPU sec, 3.706 real sec
sorting block 132, #reads = 1075094 mem = 536881520 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282130, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 334, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (349.04, 156.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676976 sequences (100000271 bp) in 2.817 CPU sec, 2.862 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282728, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 334, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (349.18, 156.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677012 sequences (100000177 bp) in 2.340 CPU sec, 2.342 real sec...
[M::mem_process_seqs] Processed 677566 reads in 179.912 CPU sec, 5.674 real sec
sorting block 133, #reads = 1075917 mem = 536879735 peak = 1164419072
[M::mem_process_seqs] Processed 677578 reads in 115.783 CPU sec, 3.646 real sec
sorting block 134, #reads = 1076489 mem = 536880275 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281854, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 337, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (351.66, 157.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677458 sequences (100000097 bp) in 2.746 CPU sec, 2.813 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281862, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 340, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (354.11, 158.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677352 sequences (100000281 bp) in 2.400 CPU sec, 2.400 real sec...
[M::mem_process_seqs] Processed 676976 reads in 182.548 CPU sec, 5.758 real sec
[M::mem_process_seqs] Processed 677012 reads in 123.579 CPU sec, 3.895 real sec
sorting block 135, #reads = 1076161 mem = 536879187 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282827, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 336, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (351.46, 157.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676812 sequences (100000292 bp) in 3.013 CPU sec, 3.202 real sec...
[M::process] read 676610 sequences (100000066 bp) in 1.793 CPU sec, 1.801 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281913, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 337, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (351.38, 156.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677458 reads in 192.349 CPU sec, 6.100 real sec
[M::mem_process_seqs] Processed 677352 reads in 131.338 CPU sec, 4.174 real sec
sorting block 136, #reads = 1074916 mem = 536880530 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282483, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 339, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (353.57, 157.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679642 sequences (100000167 bp) in 3.277 CPU sec, 3.362 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282216, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 343, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (357.38, 158.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675810 sequences (100000242 bp) in 2.275 CPU sec, 2.283 real sec...
[M::mem_process_seqs] Processed 676812 reads in 193.820 CPU sec, 6.113 real sec
sorting block 137, #reads = 1073476 mem = 536877520 peak = 1164419072
[M::mem_process_seqs] Processed 676610 reads in 127.992 CPU sec, 4.035 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283433, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 319, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (333.93, 150.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1096)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675342 sequences (100000083 bp) in 3.071 CPU sec, 3.152 real sec...
[M::process] read 675646 sequences (100000277 bp) in 2.365 CPU sec, 2.370 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281591, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 347, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (363.22, 162.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679642 reads in 209.465 CPU sec, 6.623 real sec
sorting block 138, #reads = 1073445 mem = 536880832 peak = 1164419072
[M::mem_process_seqs] Processed 675810 reads in 131.524 CPU sec, 4.175 real sec
sorting block 139, #reads = 1068390 mem = 536879102 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281882, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 352, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (368.01, 164.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675464 sequences (100000266 bp) in 3.117 CPU sec, 3.184 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281896, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 350, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (365.36, 163.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675246 sequences (100000055 bp) in 2.286 CPU sec, 2.286 real sec...
[M::mem_process_seqs] Processed 675342 reads in 181.378 CPU sec, 5.720 real sec
[M::mem_process_seqs] Processed 675646 reads in 121.736 CPU sec, 3.837 real sec
sorting block 140, #reads = 1077057 mem = 536881210 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281379, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 354, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (370.11, 166.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675070 sequences (100000114 bp) in 3.295 CPU sec, 3.410 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 282033, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 350, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (366.55, 164.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674780 sequences (100000122 bp) in 2.300 CPU sec, 2.297 real sec...
[M::mem_process_seqs] Processed 675246 reads in 181.497 CPU sec, 5.714 real sec
[M::mem_process_seqs] Processed 675464 reads in 202.891 CPU sec, 6.409 real sec
sorting block 141, #reads = 1075808 mem = 536877996 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281469, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 356, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (372.75, 168.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674666 sequences (100000055 bp) in 2.792 CPU sec, 2.847 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281040, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 360, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (376.69, 169.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674668 sequences (100000216 bp) in 2.300 CPU sec, 2.301 real sec...
[M::mem_process_seqs] Processed 675070 reads in 180.056 CPU sec, 5.680 real sec
sorting block 142, #reads = 1075452 mem = 536878739 peak = 1164419072
[M::mem_process_seqs] Processed 674780 reads in 120.129 CPU sec, 3.788 real sec
sorting block 143, #reads = 1075708 mem = 536878399 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281723, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 359, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (376.64, 169.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674786 sequences (100000268 bp) in 2.857 CPU sec, 2.913 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281518, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 360, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (376.96, 170.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674658 sequences (100000072 bp) in 2.316 CPU sec, 2.321 real sec...
[M::mem_process_seqs] Processed 674666 reads in 178.549 CPU sec, 5.632 real sec
[M::mem_process_seqs] Processed 674668 reads in 118.971 CPU sec, 3.750 real sec
sorting block 144, #reads = 1076323 mem = 536880652 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281175, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 359, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (376.48, 171.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675112 sequences (100000145 bp) in 2.884 CPU sec, 2.935 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281664, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 359, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (378.01, 172.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679136 sequences (100000186 bp) in 2.350 CPU sec, 2.346 real sec...
[M::mem_process_seqs] Processed 674786 reads in 187.087 CPU sec, 5.912 real sec
[M::mem_process_seqs] Processed 674658 reads in 126.830 CPU sec, 3.998 real sec
sorting block 145, #reads = 1075408 mem = 536881289 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281209, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 353, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (371.60, 169.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677840 sequences (100000004 bp) in 3.172 CPU sec, 3.314 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282627, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 327, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (347.94, 164.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 82, 1400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4194)
[M::mem_pestat] mean and std.dev: (681.90, 834.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5591)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677330 sequences (100000029 bp) in 2.300 CPU sec, 2.306 real sec...
[M::mem_process_seqs] Processed 675112 reads in 213.227 CPU sec, 6.762 real sec
[M::mem_process_seqs] Processed 679136 reads in 137.205 CPU sec, 4.334 real sec
sorting block 146, #reads = 1070619 mem = 536877702 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282244, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 340, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (358.89, 168.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676862 sequences (100000278 bp) in 3.271 CPU sec, 3.435 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282197, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 344, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (362.84, 169.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677840 reads in 169.125 CPU sec, 5.373 real sec
sorting block 147, #reads = 1072592 mem = 536881826 peak = 1164419072
[M::mem_process_seqs] Processed 677330 reads in 113.360 CPU sec, 3.570 real sec
[M::process] read 676878 sequences (100000030 bp) in 2.730 CPU sec, 2.784 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282176, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 347, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (365.16, 169.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 148, #reads = 1079985 mem = 536878514 peak = 1164419072
[M::process] read 676868 sequences (100000097 bp) in 2.927 CPU sec, 3.018 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281126, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 346, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (363.29, 167.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676862 reads in 174.929 CPU sec, 5.541 real sec
[M::mem_process_seqs] Processed 676878 reads in 118.715 CPU sec, 3.727 real sec
[M::process] read 676454 sequences (100000182 bp) in 2.613 CPU sec, 2.637 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282340, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 345, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (363.00, 166.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 149, #reads = 1079406 mem = 536877072 peak = 1164419072
[M::process] read 675324 sequences (100000213 bp) in 3.133 CPU sec, 3.318 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282279, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 349, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (365.23, 166.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676868 reads in 176.107 CPU sec, 5.586 real sec
[M::process] read 675616 sequences (100000009 bp) in 2.550 CPU sec, 2.574 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281699, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 354, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (368.68, 164.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675280 sequences (100000103 bp) in 2.309 CPU sec, 2.318 real sec...
[M::mem_process_seqs] Processed 675324 reads in 94.544 CPU sec, 2.975 real sec
sorting block 150, #reads = 1078344 mem = 536878950 peak = 1164419072
[M::mem_process_seqs] Processed 676454 reads in 246.093 CPU sec, 7.770 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280895, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 353, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (368.52, 166.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 151, #reads = 1078866 mem = 536881625 peak = 1164419072
[M::process] read 675116 sequences (100000225 bp) in 2.897 CPU sec, 3.019 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282231, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 356, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (370.79, 166.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675616 reads in 187.792 CPU sec, 5.951 real sec
[M::mem_process_seqs] Processed 675280 reads in 114.087 CPU sec, 3.590 real sec
[M::process] read 675374 sequences (100000198 bp) in 2.795 CPU sec, 2.873 real sec...
sorting block 152, #reads = 1079094 mem = 536880944 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282149, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 358, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (372.28, 166.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675490 sequences (100000143 bp) in 2.728 CPU sec, 2.802 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281493, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 359, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (373.83, 169.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675116 reads in 166.335 CPU sec, 5.279 real sec
[M::mem_process_seqs] Processed 675374 reads in 112.009 CPU sec, 3.527 real sec
[M::process] read 676696 sequences (100000065 bp) in 2.645 CPU sec, 2.679 real sec...
sorting block 153, #reads = 1079259 mem = 536887347 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281591, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 357, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (372.61, 169.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677832 sequences (100000128 bp) in 2.707 CPU sec, 2.787 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281878, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 345, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (361.93, 165.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675490 reads in 171.588 CPU sec, 5.441 real sec
[M::mem_process_seqs] Processed 676696 reads in 116.461 CPU sec, 3.659 real sec
sorting block 154, #reads = 1079889 mem = 536879936 peak = 1164419072
[M::process] read 676836 sequences (100000183 bp) in 2.561 CPU sec, 2.584 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282395, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 334, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (352.53, 163.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676100 sequences (100000173 bp) in 2.871 CPU sec, 2.945 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282610, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 344, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (360.80, 164.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677832 reads in 175.840 CPU sec, 5.556 real sec
sorting block 155, #reads = 1079384 mem = 536879487 peak = 1164419072
[M::process] read 676088 sequences (100000040 bp) in 2.268 CPU sec, 2.277 real sec...
[M::mem_process_seqs] Processed 676836 reads in 115.950 CPU sec, 3.650 real sec
sorting block 156, #reads = 1079620 mem = 536881904 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282186, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 348, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (365.29, 166.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675984 sequences (100000137 bp) in 2.731 CPU sec, 2.808 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282163, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 350, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (366.43, 166.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675756 sequences (100000228 bp) in 1.701 CPU sec, 1.704 real sec...
[M::mem_process_seqs] Processed 676100 reads in 174.880 CPU sec, 5.542 real sec
[M::mem_process_seqs] Processed 676088 reads in 115.307 CPU sec, 3.648 real sec
sorting block 157, #reads = 1079637 mem = 536878904 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281528, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 351, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (367.80, 167.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675192 sequences (100000205 bp) in 2.917 CPU sec, 2.985 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282350, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 353, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (368.71, 166.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675180 sequences (100000200 bp) in 2.260 CPU sec, 2.259 real sec...
[M::mem_process_seqs] Processed 675984 reads in 179.964 CPU sec, 5.678 real sec
[M::mem_process_seqs] Processed 675756 reads in 120.469 CPU sec, 3.808 real sec
sorting block 158, #reads = 1077363 mem = 536882099 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281469, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 357, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (374.16, 169.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675040 sequences (100000297 bp) in 2.764 CPU sec, 2.844 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281540, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 355, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (372.72, 169.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675192 reads in 176.608 CPU sec, 5.578 real sec
[M::process] read 674836 sequences (100000091 bp) in 2.340 CPU sec, 2.341 real sec...
sorting block 159, #reads = 1077563 mem = 536879519 peak = 1164419072
[M::mem_process_seqs] Processed 675180 reads in 119.334 CPU sec, 3.757 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281391, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 357, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (375.63, 171.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 160, #reads = 1078041 mem = 536884658 peak = 1164419072
[M::process] read 675184 sequences (100000231 bp) in 3.056 CPU sec, 3.159 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281161, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 357, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (375.82, 171.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675040 reads in 183.110 CPU sec, 5.787 real sec
[M::process] read 676050 sequences (100000011 bp) in 2.461 CPU sec, 2.467 real sec...
[M::mem_process_seqs] Processed 674836 reads in 124.067 CPU sec, 3.915 real sec
sorting block 161, #reads = 1078457 mem = 536878627 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281969, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 351, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (369.21, 167.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675734 sequences (100000045 bp) in 2.888 CPU sec, 3.055 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282426, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 346, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (363.81, 165.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675184 reads in 195.333 CPU sec, 6.214 real sec
[M::process] read 675402 sequences (100000185 bp) in 2.510 CPU sec, 2.525 real sec...
[M::mem_process_seqs] Processed 676050 reads in 136.740 CPU sec, 4.316 real sec
sorting block 162, #reads = 1076502 mem = 536878998 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281816, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 350, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (369.41, 169.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675084 sequences (100000116 bp) in 3.197 CPU sec, 3.338 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281703, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 352, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (371.34, 170.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675734 reads in 191.700 CPU sec, 6.086 real sec
[M::process] read 675080 sequences (100000003 bp) in 2.407 CPU sec, 2.421 real sec...
[M::mem_process_seqs] Processed 675402 reads in 128.096 CPU sec, 4.053 real sec
sorting block 163, #reads = 1074580 mem = 536879717 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281809, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 354, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (373.31, 170.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675422 sequences (100000058 bp) in 2.969 CPU sec, 3.190 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281333, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 353, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (372.72, 170.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675084 reads in 189.355 CPU sec, 6.045 real sec
[M::process] read 675332 sequences (100000059 bp) in 2.626 CPU sec, 2.665 real sec...
[M::mem_process_seqs] Processed 675080 reads in 129.642 CPU sec, 4.086 real sec
sorting block 164, #reads = 1077553 mem = 536878817 peak = 1164419072
sorting block 165, #reads = 1076699 mem = 536877152 peak = 1164419072
[M::process] read 675442 sequences (100000248 bp) in 3.013 CPU sec, 3.172 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281705, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 351, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (371.25, 171.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675332 reads in 110.964 CPU sec, 3.494 real sec
[M::process] read 675120 sequences (100000168 bp) in 2.501 CPU sec, 2.517 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281403, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 353, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (372.95, 172.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282195, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 354, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (375.35, 175.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675422 reads in 297.973 CPU sec, 9.506 real sec
[M::mem_process_seqs] Processed 675442 reads in 152.041 CPU sec, 4.832 real sec
[M::process] read 675718 sequences (100000168 bp) in 2.732 CPU sec, 2.813 real sec...
sorting block 166, #reads = 1075805 mem = 536879265 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282023, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 353, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (374.53, 173.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675320 sequences (100000115 bp) in 3.056 CPU sec, 3.267 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282245, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 346, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (367.64, 170.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675120 reads in 194.582 CPU sec, 6.211 real sec
sorting block 167, #reads = 1077431 mem = 536879834 peak = 1164419072
[M::mem_process_seqs] Processed 675718 reads in 130.373 CPU sec, 4.100 real sec
[M::process] read 675458 sequences (100000266 bp) in 2.588 CPU sec, 2.616 real sec...
sorting block 168, #reads = 1074639 mem = 536880480 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281929, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 349, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (368.86, 169.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680892 sequences (100000221 bp) in 2.948 CPU sec, 3.014 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281166, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 346, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (366.91, 168.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679648 sequences (100000202 bp) in 2.250 CPU sec, 2.248 real sec...
[M::mem_process_seqs] Processed 675320 reads in 197.039 CPU sec, 6.228 real sec
[M::mem_process_seqs] Processed 675458 reads in 135.181 CPU sec, 4.275 real sec
sorting block 169, #reads = 1070836 mem = 536879840 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283573, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 317, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (340.36, 164.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678462 sequences (100000154 bp) in 2.896 CPU sec, 2.971 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282754, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 325, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (346.62, 164.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677784 sequences (100000286 bp) in 2.474 CPU sec, 2.480 real sec...
[M::mem_process_seqs] Processed 680892 reads in 204.149 CPU sec, 6.446 real sec
[M::mem_process_seqs] Processed 679648 reads in 139.660 CPU sec, 4.431 real sec
sorting block 170, #reads = 1069074 mem = 536879421 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282695, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 333, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (353.11, 165.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677468 sequences (100000137 bp) in 3.250 CPU sec, 3.389 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283069, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 340, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (357.56, 165.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678462 reads in 197.035 CPU sec, 6.292 real sec
[M::process] read 676576 sequences (100000211 bp) in 2.385 CPU sec, 2.386 real sec...
sorting block 171, #reads = 1073752 mem = 536883227 peak = 1164419072
[M::mem_process_seqs] Processed 677784 reads in 131.961 CPU sec, 4.201 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282367, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 343, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (360.19, 166.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 172, #reads = 1075328 mem = 536878715 peak = 1164419072
[M::process] read 676830 sequences (100000016 bp) in 2.962 CPU sec, 3.151 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 282299, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 348, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (363.47, 164.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677468 reads in 194.252 CPU sec, 6.193 real sec
[M::process] read 676122 sequences (100000101 bp) in 2.506 CPU sec, 2.528 real sec...
[M::mem_process_seqs] Processed 676576 reads in 129.749 CPU sec, 4.102 real sec
sorting block 173, #reads = 1075655 mem = 536883313 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282331, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 347, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (362.74, 165.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675826 sequences (100000120 bp) in 2.711 CPU sec, 2.836 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282035, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 350, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (365.71, 165.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675732 sequences (100000222 bp) in 2.280 CPU sec, 2.279 real sec...
[M::mem_process_seqs] Processed 676830 reads in 176.855 CPU sec, 5.598 real sec
[M::mem_process_seqs] Processed 676122 reads in 114.924 CPU sec, 3.620 real sec
sorting block 174, #reads = 1075021 mem = 536877942 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282669, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 354, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (367.31, 163.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675182 sequences (100000126 bp) in 2.390 CPU sec, 2.459 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282310, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 358, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (370.26, 164.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675410 sequences (100000046 bp) in 2.350 CPU sec, 2.348 real sec...
[M::mem_process_seqs] Processed 675826 reads in 175.437 CPU sec, 5.542 real sec
sorting block 175, #reads = 1075003 mem = 536880483 peak = 1164419072
[M::mem_process_seqs] Processed 675732 reads in 118.310 CPU sec, 3.739 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281841, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 359, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (372.66, 165.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676116 sequences (100000170 bp) in 2.949 CPU sec, 3.017 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281557, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 357, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (370.34, 165.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676106 sequences (100000138 bp) in 2.330 CPU sec, 2.326 real sec...
[M::mem_process_seqs] Processed 675182 reads in 178.600 CPU sec, 5.630 real sec
sorting block 176, #reads = 1074686 mem = 536882969 peak = 1164419072
[M::mem_process_seqs] Processed 675410 reads in 119.861 CPU sec, 3.776 real sec
sorting block 177, #reads = 1075148 mem = 536877719 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282050, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 348, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (363.65, 163.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675600 sequences (100000192 bp) in 2.767 CPU sec, 2.835 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281894, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 345, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (361.37, 162.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676116 reads in 175.821 CPU sec, 5.548 real sec
[M::process] read 675362 sequences (100000142 bp) in 2.350 CPU sec, 2.343 real sec...
[M::mem_process_seqs] Processed 676106 reads in 118.383 CPU sec, 3.730 real sec
sorting block 178, #reads = 1077213 mem = 536882129 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281781, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 351, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (365.52, 162.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675206 sequences (100000294 bp) in 3.012 CPU sec, 3.074 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281664, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 352, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (367.00, 163.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675600 reads in 175.753 CPU sec, 5.543 real sec
[M::process] read 674824 sequences (100000254 bp) in 2.379 CPU sec, 2.384 real sec...
[M::mem_process_seqs] Processed 675362 reads in 117.991 CPU sec, 3.708 real sec
sorting block 179, #reads = 1076753 mem = 536883885 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282143, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 352, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (366.37, 161.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675246 sequences (100000030 bp) in 2.411 CPU sec, 2.475 real sec...
[M::process] read 675302 sequences (100000129 bp) in 1.710 CPU sec, 1.710 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281435, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 352, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (366.47, 160.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675206 reads in 168.367 CPU sec, 5.331 real sec
sorting block 180, #reads = 1076824 mem = 536878862 peak = 1164419072
[M::mem_process_seqs] Processed 674824 reads in 113.427 CPU sec, 3.597 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281918, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 348, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (361.65, 158.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674904 sequences (100000233 bp) in 3.030 CPU sec, 3.096 real sec...
[M::process] read 674720 sequences (100000038 bp) in 1.710 CPU sec, 1.711 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281839, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 345, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (358.59, 156.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675246 reads in 182.427 CPU sec, 5.766 real sec
sorting block 181, #reads = 1075770 mem = 536879627 peak = 1164419072
[M::mem_process_seqs] Processed 675302 reads in 203.172 CPU sec, 6.443 real sec
sorting block 182, #reads = 1073858 mem = 536880008 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281335, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 346, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (361.44, 157.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674766 sequences (100000226 bp) in 2.716 CPU sec, 2.761 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281546, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 346, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (361.70, 158.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674956 sequences (100000267 bp) in 2.290 CPU sec, 2.289 real sec...
[M::mem_process_seqs] Processed 674904 reads in 174.085 CPU sec, 5.494 real sec
[M::mem_process_seqs] Processed 674720 reads in 116.386 CPU sec, 3.672 real sec
sorting block 183, #reads = 1075156 mem = 536879517 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281232, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 346, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (361.34, 158.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674754 sequences (100000046 bp) in 2.966 CPU sec, 3.016 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281675, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 345, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (362.06, 159.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674748 sequences (100000061 bp) in 2.274 CPU sec, 2.275 real sec...
[M::mem_process_seqs] Processed 674766 reads in 174.426 CPU sec, 5.496 real sec
[M::mem_process_seqs] Processed 674956 reads in 115.516 CPU sec, 3.645 real sec
sorting block 184, #reads = 1075942 mem = 536879151 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281295, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 346, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (362.61, 159.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674708 sequences (100000224 bp) in 2.625 CPU sec, 2.689 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280553, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 344, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (360.66, 158.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674916 sequences (100000064 bp) in 2.180 CPU sec, 2.171 real sec...
[M::mem_process_seqs] Processed 674754 reads in 171.142 CPU sec, 5.408 real sec
sorting block 185, #reads = 1077679 mem = 536877933 peak = 1164419072
[M::mem_process_seqs] Processed 674748 reads in 112.746 CPU sec, 3.555 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281382, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 344, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (360.43, 157.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674838 sequences (100000230 bp) in 2.983 CPU sec, 3.029 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281070, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 344, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (361.89, 161.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674708 reads in 172.109 CPU sec, 5.433 real sec
[M::process] read 674636 sequences (100000255 bp) in 2.338 CPU sec, 2.340 real sec...
sorting block 186, #reads = 1076964 mem = 536879442 peak = 1164419072
[M::mem_process_seqs] Processed 674916 reads in 112.439 CPU sec, 3.538 real sec
sorting block 187, #reads = 1077663 mem = 536878627 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281403, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 343, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (360.68, 159.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674618 sequences (100000195 bp) in 2.268 CPU sec, 2.310 real sec...
[M::process] read 674560 sequences (100000275 bp) in 1.710 CPU sec, 1.711 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280904, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 345, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (363.32, 161.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674838 reads in 168.060 CPU sec, 5.335 real sec
[M::mem_process_seqs] Processed 674636 reads in 110.728 CPU sec, 3.505 real sec
sorting block 188, #reads = 1078099 mem = 536879916 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281140, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 347, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (363.35, 159.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674282 sequences (100000290 bp) in 2.940 CPU sec, 2.998 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280721, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 347, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (363.28, 160.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674618 reads in 170.530 CPU sec, 5.379 real sec
[M::process] read 674424 sequences (100000067 bp) in 2.249 CPU sec, 2.251 real sec...
[M::mem_process_seqs] Processed 674560 reads in 113.442 CPU sec, 3.569 real sec
sorting block 189, #reads = 1077756 mem = 536879445 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281299, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 347, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (363.95, 160.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674446 sequences (100000077 bp) in 2.723 CPU sec, 2.790 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280990, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 348, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (365.29, 161.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674330 sequences (100000060 bp) in 2.060 CPU sec, 2.061 real sec...
[M::mem_process_seqs] Processed 674282 reads in 169.284 CPU sec, 5.346 real sec
sorting block 190, #reads = 1077875 mem = 536877546 peak = 1164419072
[M::mem_process_seqs] Processed 674424 reads in 112.636 CPU sec, 3.547 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281073, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 348, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (366.34, 163.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674480 sequences (100000187 bp) in 2.921 CPU sec, 2.974 real sec...
[M::process] read 194324 sequences (28775363 bp) in 0.830 CPU sec, 0.828 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281173, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 351, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (369.28, 164.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674446 reads in 163.863 CPU sec, 5.202 real sec
sorting block 191, #reads = 1077637 mem = 536878446 peak = 1164419072
[M::mem_process_seqs] Processed 674330 reads in 108.990 CPU sec, 3.478 real sec
sorting block 192, #reads = 1078959 mem = 536879258 peak = 1164419072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280331, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 352, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (369.27, 164.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 81077, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 343, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (360.78, 161.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674480 reads in 101.662 CPU sec, 3.299 real sec
[M::mem_process_seqs] Processed 194324 reads in 38.533 CPU sec, 1.242 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 208208488
overall: 69802332160 mem 29998.760 user 562.932 sys 1007.581 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_bR4-T1-TDNA-1_A23MHWWLT4_1.L001\tPU:L001\tSM:659_bR4-T1-TDNA-1_A23MHWWLT4_1\tLB:659_bR4-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_bR4-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_bR4-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 1007.619 sec; CPU: 30561.696 sec
sorting block 193, #reads = 1063314 mem = 530938530 peak = 1164419072
uniq header count 1
merging 194 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 11120664421
output reads: 209069869
bam_mem_sort: 194 calls 121.854 user 1.803 sys 124.551 real
bam_write: 194 calls 75.269 user 3.887 sys 99.909 real
execute: 1 calls 64.415 user 1.725 sys 1097.383 real
merge_files: 1 calls 64.387 user 1.675 sys 88.634 real
parse_chunk: 3104 calls 188.578 user 38.653 sys 236.957 real
read_chunk: 8361 calls 81.046 user 56.907 sys 955.834 real
sort_block: 1 calls 60.995 user 56.453 sys 923.002 real
write_chunk: 5255 calls 63.980 user 1.274 sys 87.468 real
overall: 3363868672 mem 3506.867 user 125.323 sys 1097.403 real
ls: cannot access '*.cram': No such file or directory