Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/2f/cdf4eaf574f8c3ce29bc7fec978af1/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/88/93ad25caa6d19e5b4671f89b9976e6/1047_JG-T1-TDNA-1_A23MHWWLT4_1/0002.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/88/93ad25caa6d19e5b4671f89b9976e6/1047_JG-T1-TDNA-1_A23MHWWLT4_1/0002.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/2f/cdf4eaf574f8c3ce29bc7fec978af1/.command.run
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1047_JG-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1718.204 CPU sec, 959.783 real sec
[M::process] read 700356 sequences (100000174 bp) in 0.951 CPU sec, 0.962 real sec...
[M::process] read 699392 sequences (100000002 bp) in 1.218 CPU sec, 1.257 real sec...
[M::process] read 698514 sequences (100000186 bp) in 1.244 CPU sec, 1.255 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291539, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (246.63, 118.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697872 sequences (100000206 bp) in 1.169 CPU sec, 1.175 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290611, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (253.57, 122.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291179, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (251.29, 121.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700356 reads in 218.749 CPU sec, 6.997 real sec
[M::mem_process_seqs] Processed 698514 reads in 139.167 CPU sec, 4.477 real sec
[M::mem_process_seqs] Processed 699392 reads in 150.799 CPU sec, 4.873 real sec
sorting block 0, #reads = 1093414 mem = 536880630 peak = 601149440
[M::process] read 697548 sequences (100000098 bp) in 1.735 CPU sec, 1.874 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290558, 5, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 200, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (55, 305)
[M::mem_pestat] mean and std.dev: (174.89, 49.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 372)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (254.73, 122.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 220, 946)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2438)
[M::mem_pestat] mean and std.dev: (409.67, 466.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3184)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 697348 sequences (100000137 bp) in 1.313 CPU sec, 1.328 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290788, 1, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 232, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (256.61, 123.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 864)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 365, 639)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1491)
[M::mem_pestat] mean and std.dev: (413.06, 217.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1917)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697872 reads in 131.449 CPU sec, 4.187 real sec
[M::process] read 698182 sequences (100000047 bp) in 1.160 CPU sec, 1.166 real sec...
sorting block 1, #reads = 1093979 mem = 536880244 peak = 896847872
[M::mem_process_seqs] Processed 697548 reads in 82.530 CPU sec, 2.616 real sec
sorting block 2, #reads = 1094899 mem = 536878551 peak = 896847872
[M::process] read 698292 sequences (100000151 bp) in 1.642 CPU sec, 1.697 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290557, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 233, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (258.40, 125.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 876)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697688 sequences (100000059 bp) in 1.250 CPU sec, 1.266 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290868, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684)
[M::mem_pestat] mean and std.dev: (255.41, 124.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291522, 10, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 321, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048)
[M::mem_pestat] mean and std.dev: (337.56, 210.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1335)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (254.06, 122.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (231, 783, 1878)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5172)
[M::mem_pestat] mean and std.dev: (907.90, 753.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6819)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 697348 reads in 189.175 CPU sec, 6.060 real sec
[M::mem_process_seqs] Processed 698182 reads in 139.642 CPU sec, 4.485 real sec
[M::mem_process_seqs] Processed 698292 reads in 151.115 CPU sec, 4.878 real sec
sorting block 3, #reads = 1094104 mem = 536880296 peak = 896847872
[M::process] read 697298 sequences (100000283 bp) in 1.916 CPU sec, 2.071 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290809, 8, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (255.45, 123.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 856)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 4, #reads = 1095102 mem = 536878047 peak = 896847872
[M::process] read 696424 sequences (100000017 bp) in 1.456 CPU sec, 1.497 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290703, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (254.92, 122.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697688 reads in 135.653 CPU sec, 4.353 real sec
[M::mem_process_seqs] Processed 697298 reads in 84.920 CPU sec, 2.691 real sec
[M::process] read 697024 sequences (100000085 bp) in 1.674 CPU sec, 1.764 real sec...
sorting block 5, #reads = 1093836 mem = 536882417 peak = 896847872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290392, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (255.17, 121.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 225, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487)
[M::mem_pestat] mean and std.dev: (192.78, 56.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 587)
[M::mem_pestat] skip orientation RR
[M::process] read 697300 sequences (100000280 bp) in 1.830 CPU sec, 1.970 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290156, 1, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.45, 118.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 510, 1133)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2947)
[M::mem_pestat] mean and std.dev: (757.57, 649.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3854)
[M::mem_pestat] skip orientation RR
[M::process] read 696428 sequences (100000259 bp) in 1.260 CPU sec, 1.263 real sec...
[M::mem_process_seqs] Processed 696424 reads in 135.450 CPU sec, 4.322 real sec
[M::mem_process_seqs] Processed 697024 reads in 86.121 CPU sec, 2.736 real sec
sorting block 6, #reads = 1093044 mem = 536880508 peak = 896847872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290142, 6, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (282, 282, 555)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1101)
[M::mem_pestat] mean and std.dev: (329.00, 139.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1374)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.15, 118.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 696038 sequences (100000124 bp) in 1.832 CPU sec, 1.979 real sec...
[M::process] read 695618 sequences (100000109 bp) in 1.330 CPU sec, 1.328 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290745, 12, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (252.77, 119.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 1017, 3052)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8800)
[M::mem_pestat] mean and std.dev: (1306.17, 1283.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11674)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 256, 1253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3383)
[M::mem_pestat] mean and std.dev: (569.79, 687.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4448)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697300 reads in 137.203 CPU sec, 4.383 real sec
[M::mem_process_seqs] Processed 696428 reads in 84.608 CPU sec, 2.687 real sec
sorting block 7, #reads = 1089784 mem = 536880374 peak = 896847872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 289770, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (253.16, 118.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 695404 sequences (100000009 bp) in 1.854 CPU sec, 1.948 real sec...
[M::process] read 695118 sequences (100000030 bp) in 1.320 CPU sec, 1.314 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290184, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (254.57, 119.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 198, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380)
[M::mem_pestat] mean and std.dev: (171.50, 32.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 459)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696038 reads in 133.342 CPU sec, 4.254 real sec
sorting block 8, #reads = 1092895 mem = 536877778 peak = 896847872
[M::mem_process_seqs] Processed 695618 reads in 83.239 CPU sec, 2.652 real sec
sorting block 9, #reads = 1092258 mem = 536882798 peak = 918204416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 289981, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 233, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (255.00, 119.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698900 sequences (100000291 bp) in 1.879 CPU sec, 2.021 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 289914, 2, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (282, 332, 555)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1101)
[M::mem_pestat] mean and std.dev: (331.64, 213.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1374)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 234, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (255.52, 119.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::process] read 698986 sequences (100000273 bp) in 1.350 CPU sec, 1.356 real sec...
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 695404 reads in 133.590 CPU sec, 4.264 real sec
[M::mem_process_seqs] Processed 695118 reads in 83.466 CPU sec, 2.654 real sec
sorting block 10, #reads = 1091944 mem = 536877623 peak = 918204416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290604, 5, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (249.89, 119.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 228, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (256.43, 114.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RR
[M::process] read 698564 sequences (100000115 bp) in 2.010 CPU sec, 2.108 real sec...
[M::process] read 698314 sequences (100000257 bp) in 1.380 CPU sec, 1.371 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290991, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (251.38, 121.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698900 reads in 134.769 CPU sec, 4.306 real sec
[M::mem_process_seqs] Processed 698986 reads in 83.761 CPU sec, 2.667 real sec
sorting block 11, #reads = 1092049 mem = 536879869 peak = 918204416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291250, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (253.48, 122.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697886 sequences (100000130 bp) in 2.018 CPU sec, 2.187 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290955, 8, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684)
[M::mem_pestat] mean and std.dev: (254.93, 124.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 330, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690)
[M::mem_pestat] mean and std.dev: (279.78, 137.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 860)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698564 reads in 132.447 CPU sec, 4.237 real sec
[M::process] read 697164 sequences (100000259 bp) in 1.450 CPU sec, 1.462 real sec...
sorting block 12, #reads = 1095223 mem = 536880344 peak = 918204416
[M::mem_process_seqs] Processed 698314 reads in 83.710 CPU sec, 2.652 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290995, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 231, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693)
[M::mem_pestat] mean and std.dev: (256.93, 125.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 13, #reads = 1095508 mem = 536880531 peak = 918204416
[M::process] read 697142 sequences (100000196 bp) in 2.226 CPU sec, 2.379 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 290449, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 233, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (259.48, 126.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 880)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697712 sequences (100000122 bp) in 1.380 CPU sec, 1.381 real sec...
[M::mem_process_seqs] Processed 697886 reads in 132.447 CPU sec, 4.225 real sec
[M::mem_process_seqs] Processed 697164 reads in 83.921 CPU sec, 2.661 real sec
sorting block 14, #reads = 1094187 mem = 536879226 peak = 918204416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 290734, 1, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (249, 273, 613)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1341)
[M::mem_pestat] mean and std.dev: (410.47, 274.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1705)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 233, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (258.45, 125.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 872)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697536 sequences (100000013 bp) in 2.271 CPU sec, 2.394 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291252, 6, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 232, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1077)
[M::mem_pestat] mean and std.dev: (247.55, 117.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1385)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (255.19, 123.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697142 reads in 133.000 CPU sec, 4.259 real sec
[M::mem_process_seqs] Processed 697712 reads in 84.929 CPU sec, 2.686 real sec
[M::process] read 696926 sequences (100000198 bp) in 1.854 CPU sec, 1.915 real sec...
sorting block 15, #reads = 1093682 mem = 536879328 peak = 918204416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290543, 6, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (254.91, 123.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 419, 885)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2297)
[M::mem_pestat] mean and std.dev: (393.00, 288.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3003)
[M::mem_pestat] skip orientation RR
[M::process] read 696950 sequences (100000061 bp) in 2.059 CPU sec, 2.208 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290387, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (254.76, 121.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697536 reads in 134.897 CPU sec, 4.303 real sec
[M::process] read 697510 sequences (100000176 bp) in 1.400 CPU sec, 1.395 real sec...
[M::mem_process_seqs] Processed 696926 reads in 84.807 CPU sec, 2.686 real sec
sorting block 16, #reads = 1093192 mem = 536881858 peak = 918204416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290474, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.44, 120.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698512 sequences (100000048 bp) in 2.095 CPU sec, 2.228 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290467, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.48, 119.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (369, 581, 3635)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10167)
[M::mem_pestat] mean and std.dev: (1928.80, 2758.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 13433)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696950 reads in 134.802 CPU sec, 4.305 real sec
[M::process] read 696716 sequences (100000266 bp) in 1.429 CPU sec, 1.428 real sec...
sorting block 17, #reads = 1094319 mem = 536884195 peak = 918204416
[M::mem_process_seqs] Processed 697510 reads in 85.094 CPU sec, 2.693 real sec
sorting block 18, #reads = 1093894 mem = 536883328 peak = 970629120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290888, 2, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (249.44, 118.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 273, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (291.77, 145.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] skip orientation RR
[M::process] read 696644 sequences (100000019 bp) in 2.134 CPU sec, 2.228 real sec...
[M::process] read 696084 sequences (100000256 bp) in 1.410 CPU sec, 1.413 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290359, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.64, 119.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698512 reads in 138.571 CPU sec, 4.414 real sec
[M::mem_process_seqs] Processed 696716 reads in 85.047 CPU sec, 2.702 real sec
sorting block 19, #reads = 1090863 mem = 536880166 peak = 970629120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290292, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (253.16, 119.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 695626 sequences (100000124 bp) in 2.089 CPU sec, 2.267 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290286, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 231, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (253.73, 119.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696644 reads in 133.992 CPU sec, 4.292 real sec
[M::process] read 695484 sequences (100000227 bp) in 1.541 CPU sec, 1.562 real sec...
sorting block 20, #reads = 1092093 mem = 536878115 peak = 970629120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 289706, 6, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (314, 469, 718)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1526)
[M::mem_pestat] mean and std.dev: (455.78, 228.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1930)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (254.85, 119.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698796 sequences (100000296 bp) in 1.551 CPU sec, 1.565 real sec...
[M::mem_process_seqs] Processed 695626 reads in 79.794 CPU sec, 2.691 real sec
[M::mem_process_seqs] Processed 696084 reads in 190.115 CPU sec, 6.203 real sec
sorting block 21, #reads = 1091965 mem = 536881152 peak = 970629120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 289926, 10, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 234, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (256.56, 121.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 294, 1017)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2731)
[M::mem_pestat] mean and std.dev: (556.00, 529.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3588)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 231, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (230.00, 79.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 699064 sequences (100000153 bp) in 1.969 CPU sec, 2.100 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290756, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (250.82, 120.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 695484 reads in 146.812 CPU sec, 4.674 real sec
[M::mem_process_seqs] Processed 698796 reads in 86.336 CPU sec, 2.724 real sec
[M::process] read 698232 sequences (100000226 bp) in 1.574 CPU sec, 1.610 real sec...
sorting block 22, #reads = 1092515 mem = 536882635 peak = 970629120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291238, 10, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.69, 120.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (284, 313, 584)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1184)
[M::mem_pestat] mean and std.dev: (418.80, 223.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1484)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 697534 sequences (100000250 bp) in 2.198 CPU sec, 2.356 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291246, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (253.83, 122.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696852 sequences (100000295 bp) in 1.370 CPU sec, 1.367 real sec...
[M::mem_process_seqs] Processed 699064 reads in 133.111 CPU sec, 4.240 real sec
[M::mem_process_seqs] Processed 698232 reads in 83.070 CPU sec, 2.633 real sec
sorting block 23, #reads = 1091771 mem = 536880302 peak = 970629120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290791, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 232, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (256.39, 123.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 864)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 24, #reads = 1094925 mem = 536878696 peak = 970629120
[M::process] read 696540 sequences (100000184 bp) in 1.936 CPU sec, 2.036 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290718, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 234, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (258.92, 125.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 873)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697534 reads in 129.738 CPU sec, 4.141 real sec
[M::process] read 696998 sequences (100000076 bp) in 1.443 CPU sec, 1.452 real sec...
[M::mem_process_seqs] Processed 696852 reads in 81.989 CPU sec, 2.590 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290876, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 235, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (260.51, 126.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 881)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 25, #reads = 1093359 mem = 536879971 peak = 970629120
[M::process] read 698116 sequences (100000159 bp) in 2.114 CPU sec, 2.277 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290194, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 233, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (258.96, 126.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 876)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696540 reads in 128.173 CPU sec, 4.086 real sec
[M::mem_process_seqs] Processed 696998 reads in 82.410 CPU sec, 2.601 real sec
[M::process] read 697252 sequences (100000297 bp) in 1.521 CPU sec, 1.548 real sec...
sorting block 26, #reads = 1094354 mem = 536878304 peak = 970629120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291166, 9, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (253.44, 122.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696798 sequences (100000209 bp) in 1.840 CPU sec, 1.933 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290252, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (255.28, 123.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 852)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697024 sequences (100000050 bp) in 1.390 CPU sec, 1.383 real sec...
[M::mem_process_seqs] Processed 698116 reads in 129.706 CPU sec, 4.132 real sec
[M::mem_process_seqs] Processed 697252 reads in 82.314 CPU sec, 2.602 real sec
sorting block 27, #reads = 1094088 mem = 536882417 peak = 970629120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290651, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 202, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601)
[M::mem_pestat] mean and std.dev: (203.67, 88.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 749)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.40, 121.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697154 sequences (100000090 bp) in 2.345 CPU sec, 2.487 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290356, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.37, 120.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696798 reads in 129.334 CPU sec, 4.107 real sec
[M::process] read 697738 sequences (100000154 bp) in 1.472 CPU sec, 1.472 real sec...
sorting block 28, #reads = 1093543 mem = 536879214 peak = 970629120
[M::mem_process_seqs] Processed 697024 reads in 82.342 CPU sec, 2.601 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290808, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (252.60, 120.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 29, #reads = 1093580 mem = 536878076 peak = 970629120
[M::process] read 696726 sequences (100000175 bp) in 2.039 CPU sec, 2.195 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290488, 6, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (250.78, 119.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 498, 841)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2161)
[M::mem_pestat] mean and std.dev: (514.60, 370.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2821)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697154 reads in 130.561 CPU sec, 4.164 real sec
[M::process] read 696628 sequences (100000043 bp) in 1.518 CPU sec, 1.541 real sec...
[M::mem_process_seqs] Processed 697738 reads in 84.176 CPU sec, 2.653 real sec
sorting block 30, #reads = 1093325 mem = 536877462 peak = 970629120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290464, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.51, 120.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696390 sequences (100000042 bp) in 2.369 CPU sec, 2.529 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290238, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (253.33, 120.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696726 reads in 129.457 CPU sec, 4.124 real sec
[M::mem_process_seqs] Processed 696628 reads in 83.050 CPU sec, 2.620 real sec
[M::process] read 695974 sequences (100000002 bp) in 1.709 CPU sec, 1.767 real sec...
sorting block 31, #reads = 1091982 mem = 536879647 peak = 970629120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 290175, 5, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.34, 121.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 308, 1456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4050)
[M::mem_pestat] mean and std.dev: (602.15, 660.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5347)
[M::mem_pestat] skip orientation RR
[M::process] read 696222 sequences (100000203 bp) in 1.875 CPU sec, 1.979 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290079, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (254.98, 120.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696390 reads in 129.275 CPU sec, 4.130 real sec
[M::mem_process_seqs] Processed 695974 reads in 82.463 CPU sec, 2.601 real sec
[M::process] read 697286 sequences (100000033 bp) in 1.599 CPU sec, 1.638 real sec...
sorting block 32, #reads = 1091880 mem = 536880296 peak = 970629120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290236, 4, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (255.09, 121.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 360, 819)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1935)
[M::mem_pestat] mean and std.dev: (515.77, 390.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2493)
[M::mem_pestat] skip orientation RR
[M::process] read 699042 sequences (100000033 bp) in 2.284 CPU sec, 2.443 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290395, 5, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (469, 469, 1179)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2599)
[M::mem_pestat] mean and std.dev: (682.11, 393.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3309)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (252.90, 120.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696222 reads in 130.708 CPU sec, 4.157 real sec
sorting block 33, #reads = 1093148 mem = 536876445 peak = 970629120
[M::process] read 698266 sequences (100000156 bp) in 1.494 CPU sec, 1.521 real sec...
[M::mem_process_seqs] Processed 697286 reads in 83.519 CPU sec, 2.637 real sec
sorting block 34, #reads = 1091291 mem = 536876432 peak = 970629120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290959, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 396, 547)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1337)
[M::mem_pestat] mean and std.dev: (356.83, 225.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1732)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (251.16, 121.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697480 sequences (100000214 bp) in 1.853 CPU sec, 1.944 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291118, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (254.57, 123.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697040 sequences (100000045 bp) in 1.350 CPU sec, 1.351 real sec...
[M::mem_process_seqs] Processed 699042 reads in 128.718 CPU sec, 4.103 real sec
[M::mem_process_seqs] Processed 698266 reads in 81.646 CPU sec, 2.584 real sec
sorting block 35, #reads = 1094547 mem = 536880269 peak = 970629120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290664, 3, 20)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (256.01, 124.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 856)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (127, 257, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610)
[M::mem_pestat] mean and std.dev: (209.60, 96.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RR
[M::process] read 696348 sequences (100000083 bp) in 2.149 CPU sec, 2.244 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290698, 10, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 233, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (257.94, 125.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 865)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (227, 474, 3972)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11462)
[M::mem_pestat] mean and std.dev: (1458.40, 1730.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 15207)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 695866 sequences (100000223 bp) in 1.380 CPU sec, 1.376 real sec...
[M::mem_process_seqs] Processed 697480 reads in 127.370 CPU sec, 4.046 real sec
[M::mem_process_seqs] Processed 697040 reads in 80.568 CPU sec, 2.548 real sec
sorting block 36, #reads = 1093835 mem = 536877939 peak = 970629120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290617, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 235, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702)
[M::mem_pestat] mean and std.dev: (261.15, 127.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697580 sequences (100000228 bp) in 1.940 CPU sec, 2.062 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290746, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 236, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705)
[M::mem_pestat] mean and std.dev: (261.69, 127.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696348 reads in 127.732 CPU sec, 4.063 real sec
[M::process] read 696964 sequences (100000178 bp) in 1.420 CPU sec, 1.426 real sec...
sorting block 37, #reads = 1094113 mem = 536883350 peak = 970629120
[M::mem_process_seqs] Processed 695866 reads in 81.027 CPU sec, 2.558 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290571, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (254.50, 123.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 38, #reads = 1093845 mem = 536878801 peak = 980652032
[M::process] read 697238 sequences (100000078 bp) in 2.387 CPU sec, 2.547 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290139, 9, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (255.91, 123.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 856)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697580 reads in 129.164 CPU sec, 4.109 real sec
[M::process] read 697354 sequences (100000210 bp) in 1.501 CPU sec, 1.512 real sec...
[M::mem_process_seqs] Processed 696964 reads in 82.293 CPU sec, 2.594 real sec
sorting block 39, #reads = 1094092 mem = 536880258 peak = 980652032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290834, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.19, 122.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696832 sequences (100000171 bp) in 2.044 CPU sec, 2.209 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 290655, 2, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (410, 687, 1046)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2318)
[M::mem_pestat] mean and std.dev: (684.71, 386.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2954)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.86, 121.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 270, 1233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3409)
[M::mem_pestat] mean and std.dev: (542.73, 476.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4497)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697238 reads in 129.130 CPU sec, 4.115 real sec
[M::process] read 696314 sequences (100000266 bp) in 1.479 CPU sec, 1.487 real sec...
[M::mem_process_seqs] Processed 697354 reads in 82.815 CPU sec, 2.615 real sec
sorting block 40, #reads = 1093628 mem = 536876596 peak = 980652032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290557, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (252.96, 120.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696974 sequences (100000196 bp) in 2.386 CPU sec, 2.540 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 289943, 6, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (253.45, 120.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 207, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (262.22, 128.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696832 reads in 130.036 CPU sec, 4.135 real sec
[M::process] read 696762 sequences (100000278 bp) in 1.507 CPU sec, 1.536 real sec...
[M::mem_process_seqs] Processed 696314 reads in 82.856 CPU sec, 2.616 real sec
sorting block 41, #reads = 1093096 mem = 536878549 peak = 980652032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290812, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.65, 121.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 695960 sequences (100000081 bp) in 2.029 CPU sec, 2.181 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290427, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (253.11, 120.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696974 reads in 129.725 CPU sec, 4.136 real sec
sorting block 42, #reads = 1092773 mem = 536877983 peak = 980652032
[M::process] read 695632 sequences (100000071 bp) in 1.490 CPU sec, 1.513 real sec...
[M::mem_process_seqs] Processed 696762 reads in 82.822 CPU sec, 2.614 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 289796, 8, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.57, 121.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 43, #reads = 1092813 mem = 536878230 peak = 1011126272
[M::process] read 695688 sequences (100000301 bp) in 2.364 CPU sec, 2.521 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290069, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (256.35, 121.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 695960 reads in 129.240 CPU sec, 4.116 real sec
[M::mem_process_seqs] Processed 695632 reads in 82.514 CPU sec, 2.602 real sec
[M::process] read 697676 sequences (100000241 bp) in 1.595 CPU sec, 1.624 real sec...
sorting block 44, #reads = 1092141 mem = 536879504 peak = 1011126272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290311, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (256.71, 122.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 705164 sequences (100000243 bp) in 1.880 CPU sec, 1.970 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291133, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.91, 121.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 250, 1186)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3216)
[M::mem_pestat] mean and std.dev: (466.11, 483.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4231)
[M::mem_pestat] skip orientation RR
[M::process] read 704252 sequences (100000112 bp) in 1.404 CPU sec, 1.415 real sec...
[M::mem_process_seqs] Processed 695688 reads in 130.956 CPU sec, 4.166 real sec
[M::mem_process_seqs] Processed 697676 reads in 83.606 CPU sec, 2.650 real sec
sorting block 45, #reads = 1092384 mem = 536878140 peak = 1011126272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 293556, 9, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 209, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614)
[M::mem_pestat] mean and std.dev: (234.88, 114.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 769)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703394 sequences (100000015 bp) in 2.232 CPU sec, 2.332 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292593, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (237.70, 116.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 782)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701866 sequences (100000081 bp) in 1.400 CPU sec, 1.397 real sec...
[M::mem_process_seqs] Processed 705164 reads in 135.971 CPU sec, 4.318 real sec
sorting block 46, #reads = 1093183 mem = 536879388 peak = 1011126272
[M::mem_process_seqs] Processed 704252 reads in 85.278 CPU sec, 2.700 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292692, 1, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 419, 843)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2173)
[M::mem_pestat] mean and std.dev: (520.18, 413.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2838)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (241.13, 118.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 47, #reads = 1095961 mem = 536879250 peak = 1037828096
[M::process] read 700896 sequences (100000036 bp) in 2.069 CPU sec, 2.204 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292616, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (245.50, 120.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703394 reads in 137.332 CPU sec, 4.378 real sec
[M::process] read 701988 sequences (100000081 bp) in 1.464 CPU sec, 1.468 real sec...
[M::mem_process_seqs] Processed 701866 reads in 86.418 CPU sec, 2.737 real sec
sorting block 48, #reads = 1093876 mem = 536877185 peak = 1037828096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291940, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (247.72, 121.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703206 sequences (100000188 bp) in 2.463 CPU sec, 2.622 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292184, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (243.92, 118.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700896 reads in 134.415 CPU sec, 4.275 real sec
[M::mem_process_seqs] Processed 701988 reads in 86.048 CPU sec, 2.714 real sec
[M::process] read 703118 sequences (100000080 bp) in 1.631 CPU sec, 1.661 real sec...
sorting block 49, #reads = 1093791 mem = 536881131 peak = 1037828096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292239, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (239.49, 116.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702154 sequences (100000252 bp) in 1.876 CPU sec, 1.964 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292777, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (240.41, 117.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700772 sequences (100000010 bp) in 1.390 CPU sec, 1.394 real sec...
[M::mem_process_seqs] Processed 703206 reads in 140.681 CPU sec, 4.487 real sec
[M::mem_process_seqs] Processed 703118 reads in 89.389 CPU sec, 2.828 real sec
sorting block 50, #reads = 1094311 mem = 536876673 peak = 1037828096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 292168, 5, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 212, 292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564)
[M::mem_pestat] mean and std.dev: (227.92, 113.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 700)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (243.39, 118.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 702136 sequences (100000063 bp) in 2.061 CPU sec, 2.154 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291801, 9, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (246.60, 119.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700462 sequences (100000200 bp) in 1.430 CPU sec, 1.432 real sec...
[M::mem_process_seqs] Processed 702154 reads in 139.357 CPU sec, 4.432 real sec
sorting block 51, #reads = 1093023 mem = 536877290 peak = 1037828096
[M::mem_process_seqs] Processed 700772 reads in 86.684 CPU sec, 2.754 real sec
sorting block 52, #reads = 1092185 mem = 536877872 peak = 1113432064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292293, 8, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.80, 116.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (309, 488, 4041)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11505)
[M::mem_pestat] mean and std.dev: (1718.23, 2257.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 15237)
[M::mem_pestat] skip orientation RR
[M::process] read 699352 sequences (100000023 bp) in 1.921 CPU sec, 2.022 real sec...
[M::process] read 699260 sequences (100000262 bp) in 1.410 CPU sec, 1.408 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291811, 4, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (253, 334, 951)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2347)
[M::mem_pestat] mean and std.dev: (545.00, 410.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3045)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (245.71, 118.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 371, 1039)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2601)
[M::mem_pestat] mean and std.dev: (592.53, 386.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3382)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 702136 reads in 151.229 CPU sec, 4.818 real sec
[M::mem_process_seqs] Processed 700462 reads in 94.536 CPU sec, 3.007 real sec
sorting block 53, #reads = 1087827 mem = 536881840 peak = 1113432064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291260, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (248.10, 119.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699368 sequences (100000262 bp) in 2.410 CPU sec, 2.541 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291311, 10, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 498, 1167)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3087)
[M::mem_pestat] mean and std.dev: (508.73, 382.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4047)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.22, 120.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (64, 78, 126)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 250)
[M::mem_pestat] mean and std.dev: (74.44, 44.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 312)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::process] read 701680 sequences (100000245 bp) in 1.460 CPU sec, 1.457 real sec...
[M::mem_process_seqs] Processed 699352 reads in 143.634 CPU sec, 4.562 real sec
[M::mem_process_seqs] Processed 699260 reads in 91.426 CPU sec, 2.896 real sec
sorting block 54, #reads = 1088260 mem = 536879459 peak = 1113432064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291142, 7, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (247.01, 118.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 812)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 331, 790)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1868)
[M::mem_pestat] mean and std.dev: (503.15, 386.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2407)
[M::mem_pestat] skip orientation RR
[M::process] read 708474 sequences (100000184 bp) in 2.066 CPU sec, 2.183 real sec...
[M::process] read 704708 sequences (100000134 bp) in 1.450 CPU sec, 1.444 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291973, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (242.12, 117.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699368 reads in 147.347 CPU sec, 4.688 real sec
sorting block 55, #reads = 1088301 mem = 536881515 peak = 1113432064
[M::mem_process_seqs] Processed 701680 reads in 93.480 CPU sec, 2.966 real sec
sorting block 56, #reads = 1088538 mem = 536878367 peak = 1113432064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 294204, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 203, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589)
[M::mem_pestat] mean and std.dev: (227.40, 109.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 737)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704142 sequences (100000167 bp) in 2.328 CPU sec, 2.424 real sec...
[M::process] read 702842 sequences (100000003 bp) in 1.430 CPU sec, 1.433 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 293589, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 209, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (234.88, 113.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 708474 reads in 151.957 CPU sec, 4.829 real sec
[M::mem_process_seqs] Processed 704708 reads in 93.283 CPU sec, 2.962 real sec
sorting block 57, #reads = 1088970 mem = 536880771 peak = 1113432064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 293185, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 212, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627)
[M::mem_pestat] mean and std.dev: (237.90, 115.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 786)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701476 sequences (100000294 bp) in 2.110 CPU sec, 2.238 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 293024, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (242.29, 117.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 804)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702892 sequences (100000021 bp) in 1.420 CPU sec, 1.425 real sec...
[M::mem_process_seqs] Processed 704142 reads in 145.781 CPU sec, 4.642 real sec
[M::mem_process_seqs] Processed 702842 reads in 91.178 CPU sec, 2.888 real sec
sorting block 58, #reads = 1090614 mem = 536884391 peak = 1113432064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291932, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (244.84, 119.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702560 sequences (100000120 bp) in 2.280 CPU sec, 2.390 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292784, 5, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (240.27, 116.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 827, 2242)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6308)
[M::mem_pestat] mean and std.dev: (1346.40, 1509.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8341)
[M::mem_pestat] skip orientation RR
[M::process] read 702174 sequences (100000105 bp) in 1.420 CPU sec, 1.419 real sec...
[M::mem_process_seqs] Processed 701476 reads in 138.423 CPU sec, 4.398 real sec
sorting block 59, #reads = 1091340 mem = 536882432 peak = 1113432064
[M::mem_process_seqs] Processed 702892 reads in 87.420 CPU sec, 2.767 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292229, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (240.71, 116.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 60, #reads = 1093967 mem = 536879714 peak = 1113432064
[M::process] read 701668 sequences (100000060 bp) in 2.061 CPU sec, 2.173 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292093, 6, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.91, 116.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 271, 276)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498)
[M::mem_pestat] mean and std.dev: (230.82, 79.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 609)
[M::mem_pestat] skip orientation RR
[M::process] read 702938 sequences (100000177 bp) in 1.450 CPU sec, 1.448 real sec...
[M::mem_process_seqs] Processed 702560 reads in 138.416 CPU sec, 4.409 real sec
[M::mem_process_seqs] Processed 702174 reads in 87.316 CPU sec, 2.771 real sec
sorting block 61, #reads = 1092535 mem = 536877484 peak = 1113432064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291898, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (243.16, 117.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (302, 302, 696)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1484)
[M::mem_pestat] mean and std.dev: (427.73, 237.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1878)
[M::mem_pestat] skip orientation RR
[M::process] read 699454 sequences (100000291 bp) in 2.396 CPU sec, 2.526 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291782, 3, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (122, 261, 1350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3806)
[M::mem_pestat] mean and std.dev: (673.00, 864.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5034)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (240.68, 116.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 699016 sequences (100000212 bp) in 1.400 CPU sec, 1.397 real sec...
[M::mem_process_seqs] Processed 701668 reads in 146.484 CPU sec, 4.658 real sec
[M::mem_process_seqs] Processed 702938 reads in 96.820 CPU sec, 3.069 real sec
sorting block 62, #reads = 1091823 mem = 536877894 peak = 1113432064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291348, 6, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 204, 1140)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3140)
[M::mem_pestat] mean and std.dev: (531.70, 561.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4140)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (248.04, 120.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698786 sequences (100000082 bp) in 2.045 CPU sec, 2.159 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290783, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.75, 120.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698472 sequences (100000256 bp) in 1.400 CPU sec, 1.399 real sec...
[M::mem_process_seqs] Processed 699454 reads in 138.956 CPU sec, 4.422 real sec
[M::mem_process_seqs] Processed 699016 reads in 87.417 CPU sec, 2.766 real sec
sorting block 63, #reads = 1086716 mem = 536881033 peak = 1113432064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291449, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (250.31, 122.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698030 sequences (100000261 bp) in 2.265 CPU sec, 2.348 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291254, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (251.75, 123.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702396 sequences (100000253 bp) in 1.450 CPU sec, 1.451 real sec...
[M::mem_process_seqs] Processed 698786 reads in 135.434 CPU sec, 4.300 real sec
sorting block 64, #reads = 1091255 mem = 536878771 peak = 1113432064
[M::mem_process_seqs] Processed 698472 reads in 85.096 CPU sec, 2.695 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290328, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.51, 121.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 65, #reads = 1092693 mem = 536880316 peak = 1113432064
[M::process] read 703368 sequences (100000048 bp) in 1.922 CPU sec, 2.013 real sec...
[M::process] read 702110 sequences (100000211 bp) in 1.390 CPU sec, 1.381 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 291693, 7, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (196, 334, 945)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2443)
[M::mem_pestat] mean and std.dev: (486.06, 383.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3192)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 213, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626)
[M::mem_pestat] mean and std.dev: (239.52, 116.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 784)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698030 reads in 144.959 CPU sec, 4.624 real sec
[M::mem_process_seqs] Processed 702396 reads in 95.733 CPU sec, 3.036 real sec
sorting block 66, #reads = 1088280 mem = 536881995 peak = 1113432064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292583, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (240.30, 118.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700566 sequences (100000140 bp) in 2.225 CPU sec, 2.311 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292142, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (242.74, 118.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 229, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520)
[M::mem_pestat] mean and std.dev: (182.00, 57.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 643)
[M::mem_pestat] skip orientation RR
[M::process] read 699930 sequences (100000138 bp) in 1.380 CPU sec, 1.379 real sec...
[M::mem_process_seqs] Processed 703368 reads in 130.681 CPU sec, 4.148 real sec
[M::mem_process_seqs] Processed 702110 reads in 82.943 CPU sec, 2.626 real sec
sorting block 67, #reads = 1091999 mem = 536878425 peak = 1113432064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291510, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (247.25, 120.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699758 sequences (100000164 bp) in 1.925 CPU sec, 2.014 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291955, 4, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (380, 1066, 4749)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13487)
[M::mem_pestat] mean and std.dev: (1894.55, 1988.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 17856)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (249.97, 122.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (308, 393, 1871)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4997)
[M::mem_pestat] mean and std.dev: (851.75, 1019.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6560)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 701444 sequences (100000058 bp) in 1.390 CPU sec, 1.386 real sec...
[M::mem_process_seqs] Processed 700566 reads in 129.668 CPU sec, 4.126 real sec
sorting block 68, #reads = 1094568 mem = 536884187 peak = 1113432064
[M::mem_process_seqs] Processed 699930 reads in 81.596 CPU sec, 2.583 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291789, 2, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 372, 765)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1895)
[M::mem_pestat] mean and std.dev: (308.12, 195.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2460)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (251.37, 124.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (124, 161, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499)
[M::mem_pestat] mean and std.dev: (178.44, 87.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 624)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 69, #reads = 1094633 mem = 536878054 peak = 1113432064
[M::process] read 701474 sequences (100000271 bp) in 2.288 CPU sec, 2.367 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 292168, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (245.20, 119.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699758 reads in 129.997 CPU sec, 4.133 real sec
[M::process] read 700684 sequences (100000131 bp) in 1.418 CPU sec, 1.413 real sec...
[M::mem_process_seqs] Processed 701444 reads in 82.947 CPU sec, 2.623 real sec
sorting block 70, #reads = 1094493 mem = 536876340 peak = 1113432064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292364, 7, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 194, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (39, 339)
[M::mem_pestat] mean and std.dev: (184.44, 29.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 399)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (243.14, 118.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 699608 sequences (100000094 bp) in 1.987 CPU sec, 2.084 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292126, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (246.20, 120.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 311, 1301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3513)
[M::mem_pestat] mean and std.dev: (530.67, 471.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4619)
[M::mem_pestat] skip orientation RR
[M::process] read 698648 sequences (100000151 bp) in 1.360 CPU sec, 1.367 real sec...
[M::mem_process_seqs] Processed 701474 reads in 132.320 CPU sec, 4.214 real sec
[M::mem_process_seqs] Processed 700684 reads in 84.089 CPU sec, 2.661 real sec
sorting block 71, #reads = 1095012 mem = 536880212 peak = 1113432064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291987, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (250.14, 122.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699304 sequences (100000087 bp) in 2.462 CPU sec, 2.576 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291519, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (251.06, 122.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699608 reads in 131.781 CPU sec, 4.188 real sec
[M::process] read 699194 sequences (100000079 bp) in 1.470 CPU sec, 1.485 real sec...
[M::mem_process_seqs] Processed 698648 reads in 83.206 CPU sec, 2.628 real sec
sorting block 72, #reads = 1094291 mem = 536877916 peak = 1113432064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291192, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.95, 120.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698700 sequences (100000115 bp) in 1.875 CPU sec, 1.946 real sec...
[M::process] read 699224 sequences (100000235 bp) in 1.340 CPU sec, 1.344 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291311, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 221, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (247.19, 119.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699304 reads in 137.567 CPU sec, 4.383 real sec
sorting block 73, #reads = 1094126 mem = 536885356 peak = 1113432064
[M::mem_process_seqs] Processed 699194 reads in 86.822 CPU sec, 2.757 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290839, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.17, 120.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 74, #reads = 1090537 mem = 536879684 peak = 1113432064
[M::process] read 699100 sequences (100000116 bp) in 2.431 CPU sec, 2.524 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290884, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 220, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (246.20, 119.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698908 sequences (100000227 bp) in 1.440 CPU sec, 1.437 real sec...
[M::mem_process_seqs] Processed 698700 reads in 136.342 CPU sec, 4.326 real sec
[M::mem_process_seqs] Processed 699224 reads in 87.405 CPU sec, 2.763 real sec
sorting block 75, #reads = 1091760 mem = 536879404 peak = 1113432064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290861, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (246.95, 119.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 812)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 706472 sequences (100000074 bp) in 2.029 CPU sec, 2.085 real sec...
[M::process] read 704050 sequences (100000139 bp) in 1.390 CPU sec, 1.392 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290511, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (245.95, 118.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 804)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699100 reads in 143.583 CPU sec, 4.576 real sec
[M::mem_process_seqs] Processed 698908 reads in 94.171 CPU sec, 2.988 real sec
sorting block 76, #reads = 1090217 mem = 536878198 peak = 1113432064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 293324, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 205, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (230.32, 111.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 747)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702256 sequences (100000095 bp) in 2.085 CPU sec, 2.166 real sec...
[M::process] read 701162 sequences (100000066 bp) in 1.390 CPU sec, 1.391 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 293059, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 212, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627)
[M::mem_pestat] mean and std.dev: (238.23, 116.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 786)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 706472 reads in 145.213 CPU sec, 4.622 real sec
sorting block 77, #reads = 1086014 mem = 536883543 peak = 1113432064
[M::mem_process_seqs] Processed 704050 reads in 89.880 CPU sec, 2.852 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292509, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (244.05, 120.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 78, #reads = 1091522 mem = 536879229 peak = 1113432064
[M::process] read 700182 sequences (100000032 bp) in 2.038 CPU sec, 2.125 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292168, 9, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (246.44, 120.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 5693, 5693)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16781)
[M::mem_pestat] mean and std.dev: (3015.80, 2878.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 22325)
[M::mem_pestat] skip orientation RR
[M::process] read 699874 sequences (100000039 bp) in 1.380 CPU sec, 1.384 real sec...
[M::mem_process_seqs] Processed 702256 reads in 138.571 CPU sec, 4.410 real sec
[M::mem_process_seqs] Processed 701162 reads in 87.322 CPU sec, 2.767 real sec
sorting block 79, #reads = 1092776 mem = 536878375 peak = 1113432064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291474, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (250.80, 123.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699758 sequences (100000084 bp) in 2.041 CPU sec, 2.105 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291526, 2, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (250.16, 122.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 639, 639)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1497)
[M::mem_pestat] mean and std.dev: (453.77, 282.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1926)
[M::mem_pestat] skip orientation RR
[M::process] read 698458 sequences (100000237 bp) in 1.370 CPU sec, 1.372 real sec...
[M::mem_process_seqs] Processed 700182 reads in 136.877 CPU sec, 4.362 real sec
[M::mem_process_seqs] Processed 699874 reads in 86.371 CPU sec, 2.737 real sec
sorting block 80, #reads = 1091491 mem = 536877666 peak = 1113432064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291486, 8, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (226, 842, 1517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4099)
[M::mem_pestat] mean and std.dev: (965.82, 979.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5390)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (248.75, 120.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697628 sequences (100000249 bp) in 2.059 CPU sec, 2.147 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291313, 5, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (251.16, 121.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 284, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (271.79, 123.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699758 reads in 135.442 CPU sec, 4.316 real sec
[M::process] read 697550 sequences (100000242 bp) in 1.390 CPU sec, 1.392 real sec...
[M::mem_process_seqs] Processed 698458 reads in 85.398 CPU sec, 2.699 real sec
sorting block 81, #reads = 1093016 mem = 536878211 peak = 1113432064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 290681, 6, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 234, 678)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1724)
[M::mem_pestat] mean and std.dev: (394.77, 365.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2247)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.78, 120.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (136, 302, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (246.75, 97.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 852)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 697276 sequences (100000062 bp) in 2.211 CPU sec, 2.317 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290663, 5, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 300, 1525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4161)
[M::mem_pestat] mean and std.dev: (937.67, 951.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5479)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (251.21, 119.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (387, 440, 1367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3327)
[M::mem_pestat] mean and std.dev: (718.64, 561.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4307)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 697152 sequences (100000154 bp) in 1.350 CPU sec, 1.353 real sec...
[M::mem_process_seqs] Processed 697628 reads in 138.273 CPU sec, 4.397 real sec
sorting block 82, #reads = 1092287 mem = 536877264 peak = 1113432064
[M::mem_process_seqs] Processed 697550 reads in 87.446 CPU sec, 2.771 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290444, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.42, 118.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 269, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (292.30, 145.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RR
sorting block 83, #reads = 1089766 mem = 536880275 peak = 1123201024
[M::process] read 696994 sequences (100000252 bp) in 2.010 CPU sec, 2.090 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290110, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.22, 118.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696850 sequences (100000071 bp) in 1.370 CPU sec, 1.367 real sec...
[M::mem_process_seqs] Processed 697276 reads in 134.349 CPU sec, 4.279 real sec
[M::mem_process_seqs] Processed 697152 reads in 84.902 CPU sec, 2.685 real sec
sorting block 84, #reads = 1090781 mem = 536879399 peak = 1123201024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290345, 11, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (249.85, 117.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (648, 2893, 2893)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7383)
[M::mem_pestat] mean and std.dev: (1988.91, 1054.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9628)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 696168 sequences (100000071 bp) in 2.229 CPU sec, 2.283 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290933, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 227, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (250.22, 118.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696448 sequences (100000012 bp) in 1.340 CPU sec, 1.345 real sec...
[M::mem_process_seqs] Processed 696994 reads in 131.757 CPU sec, 4.190 real sec
[M::mem_process_seqs] Processed 696850 reads in 83.258 CPU sec, 2.636 real sec
sorting block 85, #reads = 1091498 mem = 536879441 peak = 1123201024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 289719, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 229, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (251.67, 118.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697254 sequences (100000201 bp) in 2.125 CPU sec, 2.231 real sec...
[M::process] read 10054 sequences (1440743 bp) in 0.240 CPU sec, 0.242 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290254, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.70, 119.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696168 reads in 131.270 CPU sec, 4.218 real sec
sorting block 86, #reads = 1092073 mem = 536879204 peak = 1123201024
[M::mem_process_seqs] Processed 696448 reads in 82.647 CPU sec, 2.657 real sec
sorting block 87, #reads = 1091595 mem = 536878214 peak = 1137610752
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290924, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 4222, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 223, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (245.78, 117.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] (25, 50, 75) percentile: (160, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.48, 120.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (326, 348, 1011)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2381)
[M::mem_pestat] mean and std.dev: (633.30, 457.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3066)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 10054 reads in 1.306 CPU sec, 0.045 real sec
[M::mem_process_seqs] Processed 697254 reads in 74.616 CPU sec, 2.439 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 96453542
overall: 70060199936 mem 11509.033 user 401.396 sys 1293.035 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1047_JG-T1-TDNA-1_A23MHWWLT4_1.L001\tPU:L001\tSM:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tLB:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0002.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0002.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 1293.073 sec; CPU: 11910.432 sec
sorting block 88, #reads = 803027 mem = 395115108 peak = 1137610752
uniq header count 1
merging 89 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 5148376275
output reads: 96944193
bam_mem_sort: 89 calls 31.540 user 0.209 sys 31.944 real
bam_write: 89 calls 52.644 user 1.289 sys 54.384 real
execute: 1 calls 17.644 user 0.831 sys 1320.109 real
merge_files: 1 calls 17.623 user 0.812 sys 26.032 real
parse_chunk: 1420 calls 45.008 user 11.955 sys 58.040 real
read_chunk: 3835 calls 37.661 user 33.231 sys 337.359 real
sort_block: 1 calls 30.212 user 32.706 sys 326.317 real
write_chunk: 2413 calls 17.302 user 0.622 sys 25.263 real
overall: 2997960704 mem 1022.567 user 54.649 sys 1320.134 real
ls: cannot access '*.cram': No such file or directory