.command.log - Workdir - DAQ:SENTIEON_BWAMEM (1136_27F-T1-TDNA-1_A23MHWWLT4_1;L008;0001)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/55/9db859f9837f7295824c00a013bcc7/.command.log
Size: 162.3 KB
Task: DAQ:SENTIEON_BWAMEM (1136_27F-T1-TDNA-1_A23MHWWLT4_1;L008;0001)
Attempt
Content

  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/55/9db859f9837f7295824c00a013bcc7/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/5e/6ce627e95e7f92d16b295c50c4d481/1136_27F-T1-TDNA-1_A23MHWWLT4_1/0001.1136_27F-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/55/9db859f9837f7295824c00a013bcc7/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/5e/6ce627e95e7f92d16b295c50c4d481/1136_27F-T1-TDNA-1_A23MHWWLT4_1/0001.1136_27F-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
==> STAGING COMPLETE (7 inputs)

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_27F-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1538.212 CPU sec, 1251.854 real sec
[M::process] read 696258 sequences (100000099 bp) in 0.942 CPU sec, 0.951 real sec...
[M::process] read 696682 sequences (100000183 bp) in 1.232 CPU sec, 1.242 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 290097, 30, 76)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 208, 1044)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2838)
[M::mem_pestat] mean and std.dev: (460.67, 511.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3735)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 252, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (277.26, 139.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (375, 464, 1020)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2310)
[M::mem_pestat] mean and std.dev: (685.55, 569.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2963)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 297, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1138)
[M::mem_pestat] mean and std.dev: (321.57, 189.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1448)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 697894 sequences (100000257 bp) in 1.191 CPU sec, 1.213 real sec...
[M::process] read 695364 sequences (100000184 bp) in 1.129 CPU sec, 1.131 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 290953, 28, 85)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 271, 708)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1746)
[M::mem_pestat] mean and std.dev: (383.51, 373.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2265)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 251, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (277.38, 140.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (44, 374, 758)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2186)
[M::mem_pestat] mean and std.dev: (360.62, 411.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2900)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 325, 564)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1322)
[M::mem_pestat] mean and std.dev: (365.86, 223.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1701)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696258 reads in 143.297 CPU sec, 4.550 real sec
[M::mem_process_seqs] Processed 696682 reads in 79.554 CPU sec, 2.538 real sec
sorting block 0, #reads = 1087213 mem = 536879733 peak = 611999744
[M::process] read 697300 sequences (100000068 bp) in 1.515 CPU sec, 1.596 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 291322, 35, 71)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 326, 573)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1373)
[M::mem_pestat] mean and std.dev: (378.47, 280.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1773)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 246, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (271.66, 137.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 374, 1115)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2973)
[M::mem_pestat] mean and std.dev: (620.47, 724.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3902)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 371, 834)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2080)
[M::mem_pestat] mean and std.dev: (479.27, 379.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2703)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696522 sequences (100000230 bp) in 1.190 CPU sec, 1.190 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 290288, 17, 88)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (258, 313, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864)
[M::mem_pestat] mean and std.dev: (317.76, 172.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 257, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (282.20, 142.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 989)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (420, 1291, 3308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9084)
[M::mem_pestat] mean and std.dev: (1629.06, 1385.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11972)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 369, 690)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1592)
[M::mem_pestat] mean and std.dev: (461.40, 351.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2043)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 291302, 32, 87)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 263, 657)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1611)
[M::mem_pestat] mean and std.dev: (420.89, 355.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2088)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 248, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (273.91, 138.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 956)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (102, 468, 980)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2736)
[M::mem_pestat] mean and std.dev: (488.72, 495.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3614)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 316, 690)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1650)
[M::mem_pestat] mean and std.dev: (374.58, 284.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2130)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697894 reads in 175.402 CPU sec, 5.632 real sec
[M::mem_process_seqs] Processed 695364 reads in 130.576 CPU sec, 4.217 real sec
[M::mem_process_seqs] Processed 697300 reads in 141.896 CPU sec, 4.598 real sec
sorting block 1, #reads = 1088707 mem = 536877346 peak = 740974592
[M::process] read 694938 sequences (100000096 bp) in 1.811 CPU sec, 1.917 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 290892, 16, 57)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (211, 344, 774)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1900)
[M::mem_pestat] mean and std.dev: (417.79, 321.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2463)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 249, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (274.99, 138.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (307, 470, 734)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1588)
[M::mem_pestat] mean and std.dev: (446.07, 225.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2015)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 313, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1130)
[M::mem_pestat] mean and std.dev: (328.64, 167.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1442)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 2, #reads = 1087925 mem = 536878403 peak = 908255232
[M::process] read 694976 sequences (100000058 bp) in 1.482 CPU sec, 1.526 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 290066, 29, 71)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 278, 714)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1778)
[M::mem_pestat] mean and std.dev: (400.57, 344.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2310)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 253, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (277.91, 138.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (285, 426, 1304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3342)
[M::mem_pestat] mean and std.dev: (647.67, 741.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4361)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 296, 652)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1626)
[M::mem_pestat] mean and std.dev: (404.70, 328.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2113)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696522 reads in 126.931 CPU sec, 4.039 real sec
[M::process] read 694878 sequences (100000251 bp) in 1.543 CPU sec, 1.620 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 289521, 36, 79)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 352, 587)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1417)
[M::mem_pestat] mean and std.dev: (345.23, 255.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1832)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 254, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765)
[M::mem_pestat] mean and std.dev: (278.38, 138.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 964)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (244, 411, 973)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2431)
[M::mem_pestat] mean and std.dev: (463.00, 417.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3160)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 259, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054)
[M::mem_pestat] mean and std.dev: (352.74, 253.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1365)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 693900 sequences (100000120 bp) in 1.317 CPU sec, 1.325 real sec...
[M::mem_process_seqs] Processed 694976 reads in 72.262 CPU sec, 2.297 real sec
sorting block 3, #reads = 1088225 mem = 536880473 peak = 908255232
[M::mem_process_seqs] Processed 694938 reads in 174.895 CPU sec, 5.547 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 289523, 18, 66)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 271, 598)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1390)
[M::mem_pestat] mean and std.dev: (365.69, 242.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1786)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 252, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (276.47, 137.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (391, 783, 6629)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 19105)
[M::mem_pestat] mean and std.dev: (2328.17, 2804.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 25343)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 346, 602)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1424)
[M::mem_pestat] mean and std.dev: (414.97, 285.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1835)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 4, #reads = 1087064 mem = 536878811 peak = 908255232
[M::process] read 694468 sequences (100000056 bp) in 1.802 CPU sec, 1.921 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 289699, 17, 71)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 273, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1083)
[M::mem_pestat] mean and std.dev: (319.84, 213.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1388)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 255, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (278.58, 137.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 447, 1165)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3221)
[M::mem_pestat] mean and std.dev: (622.62, 635.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4249)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 286, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1065)
[M::mem_pestat] mean and std.dev: (304.64, 200.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1364)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 694878 reads in 136.573 CPU sec, 4.350 real sec
[M::process] read 694540 sequences (100000274 bp) in 1.332 CPU sec, 1.341 real sec...
[M::mem_process_seqs] Processed 693900 reads in 79.624 CPU sec, 2.525 real sec
sorting block 5, #reads = 1087797 mem = 536885588 peak = 908255232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 289783, 21, 63)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 219, 539)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1299)
[M::mem_pestat] mean and std.dev: (297.55, 247.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1679)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 253, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (276.84, 136.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 716, 1314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3546)
[M::mem_pestat] mean and std.dev: (589.00, 468.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4662)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 252, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062)
[M::mem_pestat] mean and std.dev: (288.49, 144.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1351)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696004 sequences (100000091 bp) in 1.992 CPU sec, 2.101 real sec...
[M::process] read 696380 sequences (100000009 bp) in 1.260 CPU sec, 1.262 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 289809, 21, 69)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 394, 637)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1551)
[M::mem_pestat] mean and std.dev: (393.96, 287.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2008)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 253, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (278.34, 139.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (419, 1161, 3163)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8651)
[M::mem_pestat] mean and std.dev: (1842.57, 1921.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11395)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 294, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055)
[M::mem_pestat] mean and std.dev: (306.11, 191.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1349)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 694468 reads in 126.475 CPU sec, 4.036 real sec
[M::mem_process_seqs] Processed 694540 reads in 80.044 CPU sec, 2.549 real sec
sorting block 6, #reads = 1086956 mem = 536882880 peak = 908255232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 290289, 33, 75)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (213, 321, 548)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1218)
[M::mem_pestat] mean and std.dev: (361.12, 234.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1553)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 252, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (276.59, 138.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 963)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (222, 711, 1218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3210)
[M::mem_pestat] mean and std.dev: (823.00, 690.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4206)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 287, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (283.80, 138.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696810 sequences (100000023 bp) in 1.821 CPU sec, 1.945 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 290903, 33, 71)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (236, 451, 1163)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3017)
[M::mem_pestat] mean and std.dev: (626.68, 531.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3944)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 253, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (276.99, 138.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (255, 638, 2162)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5976)
[M::mem_pestat] mean and std.dev: (1242.48, 1150.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7883)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 276, 656)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1618)
[M::mem_pestat] mean and std.dev: (434.51, 373.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2099)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696286 sequences (100000084 bp) in 1.290 CPU sec, 1.289 real sec...
[M::mem_process_seqs] Processed 696004 reads in 124.098 CPU sec, 3.954 real sec
sorting block 7, #reads = 1086438 mem = 536877330 peak = 908255232
[M::mem_process_seqs] Processed 696380 reads in 77.736 CPU sec, 2.467 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 291413, 19, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 248, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (280.57, 175.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1306)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 252, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (277.39, 140.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (367, 645, 1066)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2464)
[M::mem_pestat] mean and std.dev: (711.68, 522.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3163)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 378, 700)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1606)
[M::mem_pestat] mean and std.dev: (483.86, 333.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2059)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 8, #reads = 1089548 mem = 536881031 peak = 908255232
[M::process] read 696172 sequences (100000177 bp) in 2.073 CPU sec, 2.157 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 290881, 28, 76)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 232, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (258.26, 140.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 252, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (277.09, 139.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (361, 1116, 1482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3724)
[M::mem_pestat] mean and std.dev: (815.79, 589.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4845)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 390, 853)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2059)
[M::mem_pestat] mean and std.dev: (532.45, 464.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2662)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696088 sequences (100000215 bp) in 1.340 CPU sec, 1.337 real sec...
[M::mem_process_seqs] Processed 696810 reads in 123.378 CPU sec, 3.927 real sec
[M::mem_process_seqs] Processed 696286 reads in 77.960 CPU sec, 2.471 real sec
sorting block 9, #reads = 1090899 mem = 536875870 peak = 908255232
[M::process] read 693796 sequences (100000000 bp) in 1.914 CPU sec, 2.035 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 290885, 24, 82)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (254, 330, 771)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1805)
[M::mem_pestat] mean and std.dev: (397.40, 249.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2322)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 252, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (276.83, 138.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 963)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (483, 1250, 1789)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4401)
[M::mem_pestat] mean and std.dev: (1167.88, 815.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5707)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 338, 595)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1331)
[M::mem_pestat] mean and std.dev: (369.43, 208.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1699)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695132 sequences (100000080 bp) in 1.350 CPU sec, 1.357 real sec...
[M::mem_process_seqs] Processed 696088 reads in 72.791 CPU sec, 2.299 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 290423, 16, 78)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 343, 706)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1738)
[M::mem_pestat] mean and std.dev: (381.72, 270.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2254)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 253, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (277.77, 139.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 967)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (124, 484, 1253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3511)
[M::mem_pestat] mean and std.dev: (614.27, 666.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4640)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 277, 697)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1787)
[M::mem_pestat] mean and std.dev: (381.97, 336.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2332)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 289411, 29, 84)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (205, 315, 799)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1987)
[M::mem_pestat] mean and std.dev: (444.42, 359.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2581)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 259, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (282.74, 140.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (404, 831, 1239)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2909)
[M::mem_pestat] mean and std.dev: (719.37, 488.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3744)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 400, 586)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1260)
[M::mem_pestat] mean and std.dev: (441.73, 250.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1597)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696172 reads in 191.648 CPU sec, 6.105 real sec
sorting block 10, #reads = 1088971 mem = 536878803 peak = 908255232
[M::process] read 695774 sequences (100000183 bp) in 1.578 CPU sec, 1.585 real sec...
[M::mem_process_seqs] Processed 693796 reads in 90.846 CPU sec, 2.890 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 289719, 39, 68)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 295, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1105)
[M::mem_pestat] mean and std.dev: (375.20, 247.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1406)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 253, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759)
sorting block 11, #reads = 1089443 mem = 536881106 peak = 941166592
[M::mem_pestat] mean and std.dev: (277.25, 138.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 956)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (644, 813, 1362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2798)
[M::mem_pestat] mean and std.dev: (864.25, 487.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3516)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 276, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (314.35, 198.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 694154 sequences (100000073 bp) in 2.342 CPU sec, 2.482 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 290190, 21, 75)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (197, 267, 668)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1610)
[M::mem_pestat] mean and std.dev: (362.75, 277.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2081)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 250, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (275.51, 138.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 393, 1177)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3197)
[M::mem_pestat] mean and std.dev: (482.79, 440.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4207)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 297, 548)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1282)
[M::mem_pestat] mean and std.dev: (319.67, 224.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1649)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695132 reads in 125.436 CPU sec, 3.992 real sec
[M::process] read 695598 sequences (100000110 bp) in 1.441 CPU sec, 1.463 real sec...
[M::mem_process_seqs] Processed 695774 reads in 80.350 CPU sec, 2.544 real sec
sorting block 12, #reads = 1085584 mem = 536881868 peak = 941166592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 289815, 32, 94)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (208, 310, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1090)
[M::mem_pestat] mean and std.dev: (358.91, 233.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1384)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 256, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (279.32, 137.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 962)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (710, 996, 2262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5366)
[M::mem_pestat] mean and std.dev: (1392.07, 1383.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6927)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 329, 637)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1473)
[M::mem_pestat] mean and std.dev: (415.95, 329.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1891)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695504 sequences (100000201 bp) in 2.243 CPU sec, 2.379 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 289978, 27, 72)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 253, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (278.83, 175.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 251, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (274.83, 136.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (533, 822, 1894)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4616)
[M::mem_pestat] mean and std.dev: (914.70, 686.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5977)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 276, 575)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1299)
[M::mem_pestat] mean and std.dev: (328.29, 195.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1661)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 694154 reads in 128.203 CPU sec, 4.088 real sec
[M::process] read 696458 sequences (100000003 bp) in 1.398 CPU sec, 1.399 real sec...
sorting block 13, #reads = 1087732 mem = 536879204 peak = 941166592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 289981, 27, 64)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 229, 788)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2022)
[M::mem_pestat] mean and std.dev: (408.22, 445.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2639)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 252, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (275.88, 137.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (108, 629, 2141)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6207)
[M::mem_pestat] mean and std.dev: (915.12, 941.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8240)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 440, 835)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2031)
[M::mem_pestat] mean and std.dev: (550.97, 449.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2629)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 698102 sequences (100000094 bp) in 1.579 CPU sec, 1.582 real sec...
[M::mem_process_seqs] Processed 695504 reads in 74.445 CPU sec, 2.548 real sec
[M::mem_process_seqs] Processed 695598 reads in 178.050 CPU sec, 5.842 real sec
sorting block 14, #reads = 1087151 mem = 536877849 peak = 941166592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 290158, 45, 63)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 437, 1308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3534)
[M::mem_pestat] mean and std.dev: (660.13, 650.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4647)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 249, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (274.45, 137.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 461, 1131)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3101)
[M::mem_pestat] mean and std.dev: (620.02, 593.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4086)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 344, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1130)
[M::mem_pestat] mean and std.dev: (360.66, 218.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1433)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696990 sequences (100000064 bp) in 2.071 CPU sec, 2.197 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (95, 291536, 18, 80)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (197, 413, 564)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1298)
[M::mem_pestat] mean and std.dev: (392.32, 270.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1665)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 247, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (270.40, 134.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 481, 976)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2514)
[M::mem_pestat] mean and std.dev: (508.31, 482.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3283)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 355, 571)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1267)
[M::mem_pestat] mean and std.dev: (385.93, 256.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1615)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696458 reads in 141.326 CPU sec, 4.492 real sec
[M::mem_process_seqs] Processed 698102 reads in 82.254 CPU sec, 2.594 real sec
[M::process] read 694724 sequences (100000169 bp) in 1.659 CPU sec, 1.703 real sec...
sorting block 15, #reads = 1086787 mem = 536878431 peak = 941166592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 290444, 32, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 273, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (312.26, 173.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 251, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (273.52, 135.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 728, 3320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9552)
[M::mem_pestat] mean and std.dev: (1708.52, 2534.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12668)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 378, 631)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1407)
[M::mem_pestat] mean and std.dev: (445.86, 282.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1795)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696852 sequences (100000186 bp) in 1.930 CPU sec, 2.049 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 290090, 36, 97)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 281, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1197)
[M::mem_pestat] mean and std.dev: (353.52, 283.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1540)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 260, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (283.46, 141.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 1065, 1703)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4767)
[M::mem_pestat] mean and std.dev: (796.19, 663.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6299)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 434, 1243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3229)
[M::mem_pestat] mean and std.dev: (604.92, 554.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4222)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696990 reads in 123.405 CPU sec, 3.930 real sec
[M::mem_process_seqs] Processed 694724 reads in 78.379 CPU sec, 2.469 real sec
[M::process] read 696562 sequences (100000121 bp) in 1.572 CPU sec, 1.606 real sec...
sorting block 16, #reads = 1089222 mem = 536879611 peak = 941166592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 290784, 25, 87)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 299, 573)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1353)
[M::mem_pestat] mean and std.dev: (360.44, 287.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1743)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 249, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (273.91, 137.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (118, 494, 1589)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4531)
[M::mem_pestat] mean and std.dev: (783.92, 796.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6002)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 284, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (307.42, 147.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 17, #reads = 1086812 mem = 536878813 peak = 941166592
[M::process] read 695792 sequences (100000059 bp) in 2.107 CPU sec, 2.222 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 291072, 26, 76)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 271, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055)
[M::mem_pestat] mean and std.dev: (324.37, 208.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1349)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 251, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (275.01, 137.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (379, 1066, 4140)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11662)
[M::mem_pestat] mean and std.dev: (2136.35, 2499.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 15423)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 276, 911)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2355)
[M::mem_pestat] mean and std.dev: (464.16, 466.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3077)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696852 reads in 122.826 CPU sec, 3.918 real sec
[M::mem_process_seqs] Processed 696562 reads in 78.511 CPU sec, 2.480 real sec
[M::process] read 693866 sequences (100000174 bp) in 1.685 CPU sec, 1.734 real sec...
sorting block 18, #reads = 1089705 mem = 536880390 peak = 941166592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 290413, 31, 61)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 433, 1169)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3095)
[M::mem_pestat] mean and std.dev: (664.17, 608.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4058)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 253, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (277.59, 138.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 967)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (477, 898, 2288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5910)
[M::mem_pestat] mean and std.dev: (1217.47, 1083.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7721)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 293, 679)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1613)
[M::mem_pestat] mean and std.dev: (404.66, 322.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2080)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695436 sequences (100000256 bp) in 1.898 CPU sec, 2.028 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 289867, 28, 79)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1256)
[M::mem_pestat] mean and std.dev: (338.10, 276.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1621)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 260, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778)
[M::mem_pestat] mean and std.dev: (282.95, 139.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 525, 1162)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3138)
[M::mem_pestat] mean and std.dev: (650.60, 623.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4126)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 310, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1078)
[M::mem_pestat] mean and std.dev: (306.46, 163.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1365)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695792 reads in 123.260 CPU sec, 3.928 real sec
[M::mem_process_seqs] Processed 693866 reads in 79.319 CPU sec, 2.501 real sec
[M::process] read 695284 sequences (100000172 bp) in 1.645 CPU sec, 1.694 real sec...
sorting block 19, #reads = 1088276 mem = 536877703 peak = 941166592
[M::process] read 695576 sequences (100000082 bp) in 1.999 CPU sec, 2.118 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 290290, 23, 67)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 242, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (257.59, 169.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 251, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (275.44, 136.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 951)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (343, 660, 2840)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7834)
[M::mem_pestat] mean and std.dev: (1351.61, 1429.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10331)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 324, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (286.29, 114.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695284 reads in 67.967 CPU sec, 2.132 real sec
[M::process] read 695092 sequences (100000213 bp) in 1.631 CPU sec, 1.675 real sec...
sorting block 20, #reads = 1086662 mem = 536878379 peak = 941166592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 290174, 27, 55)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 301, 594)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1454)
[M::mem_pestat] mean and std.dev: (344.15, 246.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1884)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 252, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762)
[M::mem_pestat] mean and std.dev: (276.81, 138.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (227, 477, 802)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1952)
[M::mem_pestat] mean and std.dev: (474.87, 335.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2527)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 408, 702)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1744)
[M::mem_pestat] mean and std.dev: (464.75, 323.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2265)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 290021, 29, 65)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 300, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1190)
[M::mem_pestat] mean and std.dev: (298.39, 194.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1524)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 249, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (273.75, 136.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (231, 372, 715)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1683)
[M::mem_pestat] mean and std.dev: (498.89, 436.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2244)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 388, 994)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2564)
[M::mem_pestat] mean and std.dev: (662.03, 653.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3349)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695436 reads in 191.115 CPU sec, 6.076 real sec
[M::mem_process_seqs] Processed 695576 reads in 91.807 CPU sec, 2.903 real sec
[M::process] read 695450 sequences (100000170 bp) in 1.667 CPU sec, 1.727 real sec...
sorting block 21, #reads = 1087536 mem = 536878812 peak = 941166592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 290562, 20, 59)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 263, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (271.69, 141.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 250, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (274.45, 136.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (233, 498, 1112)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2870)
[M::mem_pestat] mean and std.dev: (679.85, 649.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3749)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 290, 578)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1354)
[M::mem_pestat] mean and std.dev: (362.62, 276.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1742)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696158 sequences (100000041 bp) in 1.983 CPU sec, 2.138 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 290579, 20, 88)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 252, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1134)
[M::mem_pestat] mean and std.dev: (299.02, 211.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1455)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 250, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (275.17, 137.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 951)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 662, 1410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3846)
[M::mem_pestat] mean and std.dev: (690.11, 743.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5064)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 272, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1166)
[M::mem_pestat] mean and std.dev: (354.17, 233.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1485)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695092 reads in 123.775 CPU sec, 3.947 real sec
[M::mem_process_seqs] Processed 695450 reads in 79.516 CPU sec, 2.509 real sec
[M::process] read 694218 sequences (100000009 bp) in 1.658 CPU sec, 1.727 real sec...
sorting block 22, #reads = 1087274 mem = 536879036 peak = 941166592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 290552, 18, 59)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 352, 679)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1667)
[M::mem_pestat] mean and std.dev: (421.75, 305.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2161)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 252, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (274.84, 135.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (123, 444, 827)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2235)
[M::mem_pestat] mean and std.dev: (466.88, 411.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2939)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 286, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (275.34, 160.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695588 sequences (100000063 bp) in 1.926 CPU sec, 2.072 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 289360, 12, 55)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (208, 297, 798)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1978)
[M::mem_pestat] mean and std.dev: (501.69, 443.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2568)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 259, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765)
[M::mem_pestat] mean and std.dev: (279.88, 137.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 963)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (299, 1467, 2281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6245)
[M::mem_pestat] mean and std.dev: (1067.91, 954.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8227)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 375, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (328.69, 184.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1310)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696158 reads in 122.098 CPU sec, 3.895 real sec
[M::mem_process_seqs] Processed 694218 reads in 77.401 CPU sec, 2.443 real sec
sorting block 23, #reads = 1088387 mem = 536879679 peak = 941166592
[M::process] read 694196 sequences (100000238 bp) in 1.681 CPU sec, 1.737 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 290546, 13, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 268, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (318.42, 207.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1306)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 254, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (275.99, 135.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (44, 108, 607)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1733)
[M::mem_pestat] mean and std.dev: (387.31, 546.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2571)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 248, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1201)
[M::mem_pestat] mean and std.dev: (273.63, 170.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1546)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 24, #reads = 1088002 mem = 536884036 peak = 976388096
[M::process] read 697244 sequences (100000279 bp) in 1.931 CPU sec, 2.050 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 289896, 27, 83)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 328, 840)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2112)
[M::mem_pestat] mean and std.dev: (402.29, 298.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2748)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 260, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (282.46, 139.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (82, 526, 843)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2365)
[M::mem_pestat] mean and std.dev: (528.80, 508.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3126)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 325, 851)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2119)
[M::mem_pestat] mean and std.dev: (493.21, 412.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2753)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695588 reads in 120.181 CPU sec, 3.833 real sec
[M::mem_process_seqs] Processed 694196 reads in 76.762 CPU sec, 2.420 real sec
[M::process] read 697094 sequences (100000207 bp) in 1.754 CPU sec, 1.829 real sec...
sorting block 25, #reads = 1089124 mem = 536880788 peak = 976388096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 290655, 22, 77)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 265, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1172)
[M::mem_pestat] mean and std.dev: (332.89, 249.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1507)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 249, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (273.39, 136.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (107, 526, 1139)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3203)
[M::mem_pestat] mean and std.dev: (598.82, 555.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4235)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 319, 746)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1842)
[M::mem_pestat] mean and std.dev: (427.77, 358.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2390)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695274 sequences (100000181 bp) in 1.893 CPU sec, 2.007 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (87, 290534, 29, 63)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 292, 669)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1693)
[M::mem_pestat] mean and std.dev: (317.21, 245.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2205)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 248, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (272.79, 136.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (241, 645, 1087)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2779)
[M::mem_pestat] mean and std.dev: (545.92, 397.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3625)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 302, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1204)
[M::mem_pestat] mean and std.dev: (339.61, 207.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1540)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697244 reads in 123.793 CPU sec, 3.951 real sec
[M::mem_process_seqs] Processed 697094 reads in 79.253 CPU sec, 2.502 real sec
[M::process] read 695636 sequences (100000128 bp) in 1.796 CPU sec, 1.866 real sec...
sorting block 26, #reads = 1088475 mem = 536879138 peak = 976388096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 290247, 23, 76)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 279, 636)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1556)
[M::mem_pestat] mean and std.dev: (387.86, 321.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2016)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 254, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765)
[M::mem_pestat] mean and std.dev: (278.20, 138.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 964)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (695, 1064, 1912)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4346)
[M::mem_pestat] mean and std.dev: (1229.27, 919.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5563)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 313, 570)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1304)
[M::mem_pestat] mean and std.dev: (369.93, 259.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1671)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 694772 sequences (100000224 bp) in 1.918 CPU sec, 2.022 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (93, 290798, 18, 59)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (219, 363, 806)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1980)
[M::mem_pestat] mean and std.dev: (508.71, 450.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2567)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 253, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762)
[M::mem_pestat] mean and std.dev: (277.35, 138.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (266, 1632, 2737)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7679)
[M::mem_pestat] mean and std.dev: (1652.17, 1604.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10150)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 393, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (361.57, 182.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1302)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695274 reads in 123.454 CPU sec, 3.934 real sec
[M::mem_process_seqs] Processed 695636 reads in 78.660 CPU sec, 2.478 real sec
sorting block 27, #reads = 1089281 mem = 536878795 peak = 984104960
[M::process] read 695542 sequences (100000062 bp) in 1.728 CPU sec, 1.788 real sec...
sorting block 28, #reads = 1089362 mem = 536878394 peak = 986939392
[M::process] read 695436 sequences (100000223 bp) in 1.998 CPU sec, 2.112 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 290045, 29, 69)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 230, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (230.91, 138.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 251, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (274.89, 136.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (411, 789, 1207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2799)
[M::mem_pestat] mean and std.dev: (663.31, 420.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3595)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 295, 612)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1420)
[M::mem_pestat] mean and std.dev: (367.48, 247.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1824)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695542 reads in 69.425 CPU sec, 2.196 real sec
[M::process] read 695204 sequences (100000062 bp) in 1.718 CPU sec, 1.788 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 289981, 30, 70)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 250, 549)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1293)
[M::mem_pestat] mean and std.dev: (353.62, 295.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1665)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 256, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (280.09, 139.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (213, 509, 805)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1989)
[M::mem_pestat] mean and std.dev: (427.58, 295.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2581)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 292, 558)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1268)
[M::mem_pestat] mean and std.dev: (373.46, 251.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1623)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 290349, 23, 81)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 220, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (252.47, 159.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 250, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (273.96, 135.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (279, 480, 1209)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3069)
[M::mem_pestat] mean and std.dev: (660.57, 661.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3999)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 247, 537)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1265)
[M::mem_pestat] mean and std.dev: (339.93, 265.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1629)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 694772 reads in 193.885 CPU sec, 6.174 real sec
[M::mem_process_seqs] Processed 695436 reads in 92.545 CPU sec, 2.931 real sec
sorting block 29, #reads = 1087600 mem = 536878015 peak = 986939392
[M::process] read 698444 sequences (100000011 bp) in 1.806 CPU sec, 1.881 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 290275, 20, 81)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 252, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (237.37, 98.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 884)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 250, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (274.80, 136.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (592, 752, 1721)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3979)
[M::mem_pestat] mean and std.dev: (1089.53, 914.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5108)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 301, 1008)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2654)
[M::mem_pestat] mean and std.dev: (479.37, 519.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3477)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 699744 sequences (100000123 bp) in 2.026 CPU sec, 2.167 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 291418, 34, 65)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 288, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (271.70, 138.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 240, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (266.71, 134.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (214, 750, 1417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3823)
[M::mem_pestat] mean and std.dev: (892.61, 888.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5026)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 315, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093)
[M::mem_pestat] mean and std.dev: (340.55, 203.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1396)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695204 reads in 128.957 CPU sec, 4.114 real sec
sorting block 30, #reads = 1086577 mem = 536887817 peak = 986939392
[M::mem_process_seqs] Processed 698444 reads in 84.036 CPU sec, 2.649 real sec
[M::process] read 701580 sequences (100000045 bp) in 1.836 CPU sec, 1.911 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 292675, 18, 71)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 278, 852)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2230)
[M::mem_pestat] mean and std.dev: (398.15, 408.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2919)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 240, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (270.17, 140.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (366, 810, 1921)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5031)
[M::mem_pestat] mean and std.dev: (1134.06, 890.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6586)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 274, 786)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1934)
[M::mem_pestat] mean and std.dev: (461.03, 447.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2508)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 31, #reads = 1087317 mem = 536878525 peak = 1050423296
[M::process] read 699426 sequences (100000264 bp) in 1.939 CPU sec, 2.067 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 292970, 26, 63)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 297, 1010)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2660)
[M::mem_pestat] mean and std.dev: (459.34, 469.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3485)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 234, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724)
[M::mem_pestat] mean and std.dev: (262.47, 135.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 913)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (369, 839, 1162)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2748)
[M::mem_pestat] mean and std.dev: (844.38, 556.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3541)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 245, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1202)
[M::mem_pestat] mean and std.dev: (290.53, 199.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1543)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699744 reads in 130.808 CPU sec, 4.161 real sec
[M::process] read 700430 sequences (100000219 bp) in 1.453 CPU sec, 1.473 real sec...
[M::mem_process_seqs] Processed 701580 reads in 83.338 CPU sec, 2.625 real sec
sorting block 32, #reads = 1088301 mem = 536879219 peak = 1050423296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 291876, 23, 80)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 287, 709)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1775)
[M::mem_pestat] mean and std.dev: (393.38, 372.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2308)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 241, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (269.00, 137.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (267, 368, 548)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1110)
[M::mem_pestat] mean and std.dev: (347.65, 213.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1391)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 316, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (316.18, 161.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 699646 sequences (100000005 bp) in 2.048 CPU sec, 2.149 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 292699, 30, 59)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (208, 303, 741)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1807)
[M::mem_pestat] mean and std.dev: (457.07, 410.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2340)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 236, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (263.97, 134.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 918)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (287, 641, 4381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12569)
[M::mem_pestat] mean and std.dev: (2052.43, 2555.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16663)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 316, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1107)
[M::mem_pestat] mean and std.dev: (329.87, 221.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1411)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699426 reads in 130.818 CPU sec, 4.163 real sec
[M::process] read 697124 sequences (100000033 bp) in 1.416 CPU sec, 1.423 real sec...
[M::mem_process_seqs] Processed 700430 reads in 83.794 CPU sec, 2.640 real sec
sorting block 33, #reads = 1088981 mem = 536888600 peak = 1050423296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 292315, 22, 56)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (310, 423, 673)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1399)
[M::mem_pestat] mean and std.dev: (477.23, 263.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1762)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 239, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (266.59, 136.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (61, 354, 934)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2680)
[M::mem_pestat] mean and std.dev: (486.95, 536.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3553)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 268, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051)
[M::mem_pestat] mean and std.dev: (317.52, 183.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1331)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696878 sequences (100000212 bp) in 1.990 CPU sec, 2.092 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 291042, 27, 73)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 353, 772)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1956)
[M::mem_pestat] mean and std.dev: (391.41, 366.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2548)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 248, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (275.94, 141.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 968)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (390, 753, 1412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3456)
[M::mem_pestat] mean and std.dev: (945.07, 827.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4478)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 311, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1097)
[M::mem_pestat] mean and std.dev: (344.63, 211.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1394)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695458 sequences (100000040 bp) in 1.390 CPU sec, 1.391 real sec...
[M::mem_process_seqs] Processed 699646 reads in 131.880 CPU sec, 4.193 real sec
sorting block 34, #reads = 1087741 mem = 536880323 peak = 1050423296
[M::process] read 696418 sequences (100000045 bp) in 1.509 CPU sec, 1.514 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 290803, 29, 75)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 322, 800)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1992)
[M::mem_pestat] mean and std.dev: (392.45, 271.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2588)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 248, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (274.57, 138.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (122, 1299, 5651)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16709)
[M::mem_pestat] mean and std.dev: (2676.34, 2639.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 22238)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 335, 716)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1640)
[M::mem_pestat] mean and std.dev: (446.82, 345.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2102)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696878 reads in 76.696 CPU sec, 2.647 real sec
sorting block 35, #reads = 1086790 mem = 536879180 peak = 1050423296
[M::mem_process_seqs] Processed 697124 reads in 178.702 CPU sec, 5.881 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 290241, 34, 72)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 283, 595)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1449)
[M::mem_pestat] mean and std.dev: (335.11, 267.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1876)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 252, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (278.19, 140.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 967)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (368, 606, 1522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3830)
[M::mem_pestat] mean and std.dev: (791.06, 705.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4984)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 361, 887)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2239)
[M::mem_pestat] mean and std.dev: (544.65, 532.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2915)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695786 sequences (100000005 bp) in 2.202 CPU sec, 2.293 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 290517, 22, 58)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 248, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1109)
[M::mem_pestat] mean and std.dev: (292.88, 210.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1423)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 248, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (273.92, 137.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 544, 853)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2169)
[M::mem_pestat] mean and std.dev: (520.76, 303.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2827)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 373, 1202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3198)
[M::mem_pestat] mean and std.dev: (606.58, 740.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4196)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695458 reads in 144.882 CPU sec, 4.600 real sec
[M::mem_process_seqs] Processed 696418 reads in 85.712 CPU sec, 2.694 real sec
[M::process] read 697744 sequences (100000042 bp) in 1.626 CPU sec, 1.673 real sec...
sorting block 36, #reads = 1085610 mem = 536882011 peak = 1050423296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 290839, 25, 76)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 211, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (232.66, 137.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 250, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (276.70, 139.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 959)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 439, 1480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4058)
[M::mem_pestat] mean and std.dev: (679.96, 803.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5347)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 317, 574)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1298)
[M::mem_pestat] mean and std.dev: (357.52, 245.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1660)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 37, #reads = 1085583 mem = 536878631 peak = 1050423296
[M::process] read 703832 sequences (100000019 bp) in 2.074 CPU sec, 2.194 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 291554, 34, 64)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 245, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1087)
[M::mem_pestat] mean and std.dev: (299.48, 195.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1394)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 244, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (271.01, 137.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (197, 647, 1437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3917)
[M::mem_pestat] mean and std.dev: (806.61, 674.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5157)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 315, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (317.93, 158.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695786 reads in 133.528 CPU sec, 4.246 real sec
[M::process] read 702376 sequences (100000222 bp) in 1.503 CPU sec, 1.510 real sec...
[M::mem_process_seqs] Processed 697744 reads in 86.325 CPU sec, 2.720 real sec
sorting block 38, #reads = 1085616 mem = 536880212 peak = 1050423296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 293954, 13, 60)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 240, 597)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1473)
[M::mem_pestat] mean and std.dev: (356.33, 313.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1911)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 228, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (255.85, 130.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (518, 1011, 2731)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7157)
[M::mem_pestat] mean and std.dev: (1636.92, 1527.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9370)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 278, 583)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1405)
[M::mem_pestat] mean and std.dev: (337.28, 268.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1816)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 700096 sequences (100000021 bp) in 2.350 CPU sec, 2.463 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 293324, 25, 56)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 219, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (288.95, 202.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 233, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (259.19, 130.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 417, 848)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2210)
[M::mem_pestat] mean and std.dev: (410.38, 320.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2891)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 273, 916)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2356)
[M::mem_pestat] mean and std.dev: (504.27, 486.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3076)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 700886 sequences (100000142 bp) in 1.440 CPU sec, 1.440 real sec...
[M::mem_process_seqs] Processed 703832 reads in 143.836 CPU sec, 4.565 real sec
[M::mem_process_seqs] Processed 702376 reads in 90.406 CPU sec, 2.860 real sec
sorting block 39, #reads = 1082698 mem = 536878158 peak = 1050423296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 292307, 23, 58)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (239, 328, 642)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1448)
[M::mem_pestat] mean and std.dev: (397.71, 286.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1851)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 238, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (264.41, 133.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 919)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (464, 638, 819)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1529)
[M::mem_pestat] mean and std.dev: (655.57, 307.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1887)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 284, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1116)
[M::mem_pestat] mean and std.dev: (299.52, 169.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1425)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 699766 sequences (100000116 bp) in 2.067 CPU sec, 2.211 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 292531, 16, 87)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 361, 1088)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2886)
[M::mem_pestat] mean and std.dev: (513.25, 404.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3785)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 235, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (261.45, 132.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 902)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (211, 711, 2078)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5812)
[M::mem_pestat] mean and std.dev: (1201.94, 1204.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7679)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 291, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1148)
[M::mem_pestat] mean and std.dev: (343.33, 232.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1469)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 697570 sequences (100000181 bp) in 1.420 CPU sec, 1.421 real sec...
[M::mem_process_seqs] Processed 700096 reads in 135.598 CPU sec, 4.307 real sec
[M::mem_process_seqs] Processed 700886 reads in 85.393 CPU sec, 2.700 real sec
sorting block 40, #reads = 1084423 mem = 536879350 peak = 1050423296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 292417, 26, 89)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 292, 597)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1371)
[M::mem_pestat] mean and std.dev: (361.00, 270.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1758)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 239, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (266.01, 134.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (214, 759, 1310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3502)
[M::mem_pestat] mean and std.dev: (877.04, 761.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4598)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 258, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (252.68, 119.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 697254 sequences (100000088 bp) in 2.354 CPU sec, 2.482 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 290828, 28, 78)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 280, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (273.17, 156.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 246, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (273.62, 139.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 956)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (117, 795, 5998)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17760)
[M::mem_pestat] mean and std.dev: (2241.21, 2697.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 23641)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 294, 553)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1269)
[M::mem_pestat] mean and std.dev: (353.65, 250.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1627)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695584 sequences (100000210 bp) in 1.440 CPU sec, 1.437 real sec...
[M::mem_process_seqs] Processed 699766 reads in 133.968 CPU sec, 4.248 real sec
sorting block 41, #reads = 1085954 mem = 536881671 peak = 1050423296
[M::mem_process_seqs] Processed 697570 reads in 84.834 CPU sec, 2.678 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 291297, 30, 59)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 246, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1206)
[M::mem_pestat] mean and std.dev: (266.58, 180.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1553)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 42, #reads = 1083986 mem = 536878714 peak = 1122066432
[M::mem_pestat] (25, 50, 75) percentile: (164, 247, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (274.66, 140.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 459, 666)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1746)
[M::mem_pestat] mean and std.dev: (408.71, 334.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2286)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (287, 532, 1392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3602)
[M::mem_pestat] mean and std.dev: (863.73, 871.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4707)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 697094 sequences (100000068 bp) in 2.128 CPU sec, 2.253 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 290585, 26, 79)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 293, 1211)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3221)
[M::mem_pestat] mean and std.dev: (539.24, 535.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4226)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 252, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (279.32, 141.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (230, 530, 619)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1397)
[M::mem_pestat] mean and std.dev: (453.00, 269.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1786)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 284, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1140)
[M::mem_pestat] mean and std.dev: (319.72, 217.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1465)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697254 reads in 131.133 CPU sec, 4.173 real sec
[M::process] read 696876 sequences (100000283 bp) in 1.532 CPU sec, 1.552 real sec...
[M::mem_process_seqs] Processed 695584 reads in 83.722 CPU sec, 2.636 real sec
sorting block 43, #reads = 1084487 mem = 536881497 peak = 1122066432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 290859, 35, 73)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (235, 317, 1258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3304)
[M::mem_pestat] mean and std.dev: (729.61, 802.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4327)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 246, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (273.32, 138.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (476, 1655, 1833)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4547)
[M::mem_pestat] mean and std.dev: (1276.00, 935.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5904)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 330, 905)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2319)
[M::mem_pestat] mean and std.dev: (518.43, 503.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3026)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 699996 sequences (100000094 bp) in 2.405 CPU sec, 2.512 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 290873, 20, 65)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 354, 802)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2064)
[M::mem_pestat] mean and std.dev: (485.16, 460.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2695)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 246, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (274.52, 140.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 956)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 214, 1930)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5482)
[M::mem_pestat] mean and std.dev: (816.68, 1323.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7258)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 287, 604)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1428)
[M::mem_pestat] mean and std.dev: (302.63, 205.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1840)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697094 reads in 131.604 CPU sec, 4.178 real sec
[M::process] read 700610 sequences (100000170 bp) in 1.652 CPU sec, 1.667 real sec...
[M::mem_process_seqs] Processed 696876 reads in 85.289 CPU sec, 2.681 real sec
sorting block 44, #reads = 1085031 mem = 536878755 peak = 1122066432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 291813, 32, 70)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 378, 933)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2443)
[M::mem_pestat] mean and std.dev: (569.75, 559.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3198)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 237, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (264.74, 134.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 919)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (236, 535, 873)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2147)
[M::mem_pestat] mean and std.dev: (504.43, 374.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2784)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 288, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1185)
[M::mem_pestat] mean and std.dev: (319.42, 219.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1516)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 699178 sequences (100000262 bp) in 2.126 CPU sec, 2.237 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 292593, 22, 55)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 289, 628)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1550)
[M::mem_pestat] mean and std.dev: (433.92, 349.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2011)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 238, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734)
[M::mem_pestat] mean and std.dev: (264.98, 134.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 926)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (218, 354, 1193)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3143)
[M::mem_pestat] mean and std.dev: (542.25, 542.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4118)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 388, 843)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2095)
[M::mem_pestat] mean and std.dev: (508.81, 392.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2721)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699996 reads in 135.886 CPU sec, 4.326 real sec
[M::process] read 697438 sequences (100000237 bp) in 1.437 CPU sec, 1.441 real sec...
sorting block 45, #reads = 1084335 mem = 536883173 peak = 1122066432
[M::mem_process_seqs] Processed 700610 reads in 82.782 CPU sec, 2.613 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 291851, 23, 59)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 217, 538)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1340)
[M::mem_pestat] mean and std.dev: (320.47, 281.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1741)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 242, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (268.64, 136.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (102, 558, 1042)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2922)
[M::mem_pestat] mean and std.dev: (606.73, 602.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3862)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 244, 671)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1673)
[M::mem_pestat] mean and std.dev: (415.57, 420.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2174)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 46, #reads = 1084840 mem = 536878656 peak = 1122066432
[M::process] read 700130 sequences (100000087 bp) in 2.454 CPU sec, 2.568 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 291673, 31, 58)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 285, 953)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2553)
[M::mem_pestat] mean and std.dev: (439.46, 453.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3353)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 248, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (274.19, 138.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (386, 652, 1943)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5057)
[M::mem_pestat] mean and std.dev: (979.41, 1237.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6614)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 242, 886)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2292)
[M::mem_pestat] mean and std.dev: (453.93, 414.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2995)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699178 reads in 126.350 CPU sec, 4.012 real sec
[M::mem_process_seqs] Processed 697438 reads in 81.093 CPU sec, 2.555 real sec
[M::process] read 699040 sequences (100000213 bp) in 1.787 CPU sec, 1.851 real sec...
sorting block 47, #reads = 1087735 mem = 536878901 peak = 1122066432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 292381, 22, 56)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 283, 565)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1299)
[M::mem_pestat] mean and std.dev: (334.17, 229.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1666)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 237, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (262.85, 131.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 1324, 1831)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5105)
[M::mem_pestat] mean and std.dev: (1382.59, 1491.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7347)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 280, 744)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1868)
[M::mem_pestat] mean and std.dev: (483.72, 456.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2430)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 697566 sequences (100000110 bp) in 1.965 CPU sec, 2.100 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 292099, 21, 59)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (199, 273, 717)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1753)
[M::mem_pestat] mean and std.dev: (314.39, 261.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2271)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 241, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (266.55, 133.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (249, 705, 986)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2460)
[M::mem_pestat] mean and std.dev: (643.62, 473.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3197)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 350, 675)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1551)
[M::mem_pestat] mean and std.dev: (410.22, 341.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1989)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700130 reads in 128.718 CPU sec, 4.102 real sec
[M::mem_process_seqs] Processed 699040 reads in 81.839 CPU sec, 2.575 real sec
[M::process] read 698340 sequences (100000127 bp) in 1.731 CPU sec, 1.772 real sec...
sorting block 48, #reads = 1087875 mem = 536880971 peak = 1122066432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 291893, 37, 104)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 283, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1268)
[M::mem_pestat] mean and std.dev: (307.61, 229.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1636)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 247, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (273.72, 138.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 531, 1325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3667)
[M::mem_pestat] mean and std.dev: (837.88, 1114.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5296)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 369, 785)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1987)
[M::mem_pestat] mean and std.dev: (444.01, 341.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2588)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 697906 sequences (100000235 bp) in 2.357 CPU sec, 2.472 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 291364, 21, 68)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 229, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (234.23, 127.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 951)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 242, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (268.10, 134.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 926)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (256, 607, 1248)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3232)
[M::mem_pestat] mean and std.dev: (612.68, 487.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4224)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 269, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1142)
[M::mem_pestat] mean and std.dev: (325.61, 215.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1462)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697566 reads in 128.164 CPU sec, 4.080 real sec
[M::mem_process_seqs] Processed 698340 reads in 82.204 CPU sec, 2.597 real sec
sorting block 49, #reads = 1088536 mem = 536880299 peak = 1122066432
[M::process] read 697884 sequences (100000058 bp) in 1.936 CPU sec, 2.032 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 291584, 31, 70)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 381, 729)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1845)
[M::mem_pestat] mean and std.dev: (467.56, 395.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2403)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 244, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (271.03, 136.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (108, 229, 1024)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2856)
[M::mem_pestat] mean and std.dev: (458.04, 543.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3772)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 328, 558)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1268)
[M::mem_pestat] mean and std.dev: (341.43, 190.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1623)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 50, #reads = 1086748 mem = 536880698 peak = 1122066432
[M::process] read 697852 sequences (100000100 bp) in 1.986 CPU sec, 2.106 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 291224, 31, 61)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 273, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1100)
[M::mem_pestat] mean and std.dev: (312.66, 203.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1403)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 244, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (271.84, 138.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (290, 880, 1644)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4352)
[M::mem_pestat] mean and std.dev: (999.89, 1049.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5706)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 271, 649)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1597)
[M::mem_pestat] mean and std.dev: (355.71, 267.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2071)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697906 reads in 131.270 CPU sec, 4.186 real sec
[M::mem_process_seqs] Processed 697884 reads in 84.888 CPU sec, 2.676 real sec
[M::process] read 698926 sequences (100000212 bp) in 1.887 CPU sec, 1.960 real sec...
sorting block 51, #reads = 1086735 mem = 536879962 peak = 1122066432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 291178, 30, 64)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 222, 612)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1504)
[M::mem_pestat] mean and std.dev: (357.72, 291.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1950)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 243, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (271.49, 138.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (102, 780, 2813)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8235)
[M::mem_pestat] mean and std.dev: (1401.63, 1686.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10946)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 236, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (256.12, 124.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 884)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 704598 sequences (100000137 bp) in 2.307 CPU sec, 2.419 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 291192, 31, 62)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 329, 916)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2442)
[M::mem_pestat] mean and std.dev: (549.80, 560.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3205)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 239, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (265.90, 134.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 913)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (268, 640, 1707)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4585)
[M::mem_pestat] mean and std.dev: (832.43, 758.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6024)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 306, 786)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1934)
[M::mem_pestat] mean and std.dev: (403.68, 362.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2508)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697852 reads in 139.142 CPU sec, 4.422 real sec
[M::mem_process_seqs] Processed 698926 reads in 92.325 CPU sec, 2.903 real sec
[M::process] read 703564 sequences (100000298 bp) in 1.700 CPU sec, 1.740 real sec...
sorting block 52, #reads = 1085546 mem = 536879172 peak = 1122066432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 293146, 16, 65)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (199, 342, 643)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1531)
[M::mem_pestat] mean and std.dev: (397.86, 289.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1975)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 228, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (255.97, 130.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 897)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (388, 721, 2627)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7105)
[M::mem_pestat] mean and std.dev: (1253.44, 1339.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9344)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 332, 559)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1311)
[M::mem_pestat] mean and std.dev: (377.21, 248.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1687)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 701678 sequences (100000147 bp) in 2.134 CPU sec, 2.245 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 294083, 29, 62)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (203, 591, 861)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2177)
[M::mem_pestat] mean and std.dev: (571.78, 450.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2835)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 229, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (256.02, 129.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (542, 778, 2713)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7055)
[M::mem_pestat] mean and std.dev: (1307.93, 1162.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9226)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 302, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (284.76, 143.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 702890 sequences (100000142 bp) in 1.470 CPU sec, 1.462 real sec...
[M::mem_process_seqs] Processed 704598 reads in 146.267 CPU sec, 4.652 real sec
[M::mem_process_seqs] Processed 703564 reads in 91.820 CPU sec, 2.904 real sec
sorting block 53, #reads = 1081690 mem = 536877917 peak = 1122066432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 293025, 13, 75)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 282, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1104)
[M::mem_pestat] mean and std.dev: (312.82, 204.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1413)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 236, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (263.84, 134.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 644, 2061)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5867)
[M::mem_pestat] mean and std.dev: (823.42, 794.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7770)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 280, 783)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2015)
[M::mem_pestat] mean and std.dev: (496.27, 473.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2631)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 700872 sequences (100000238 bp) in 2.279 CPU sec, 2.405 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 293269, 27, 55)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 306, 713)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1765)
[M::mem_pestat] mean and std.dev: (413.16, 361.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2291)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 229, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (255.26, 127.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (82, 1009, 2975)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8761)
[M::mem_pestat] mean and std.dev: (1945.31, 2702.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12757)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 336, 905)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2323)
[M::mem_pestat] mean and std.dev: (531.23, 495.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3032)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 698650 sequences (100000083 bp) in 1.510 CPU sec, 1.505 real sec...
[M::mem_process_seqs] Processed 701678 reads in 138.135 CPU sec, 4.379 real sec
sorting block 54, #reads = 1083651 mem = 536878614 peak = 1122066432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 292203, 29, 65)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 245, 841)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2143)
[M::mem_pestat] mean and std.dev: (503.06, 525.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2794)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 235, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (261.16, 130.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 902)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 404, 1217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3255)
[M::mem_pestat] mean and std.dev: (691.89, 722.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4274)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 324, 650)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1510)
[M::mem_pestat] mean and std.dev: (427.17, 343.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1940)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 698916 sequences (100000040 bp) in 1.633 CPU sec, 1.640 real sec...
[M::mem_process_seqs] Processed 700872 reads in 78.763 CPU sec, 2.703 real sec
sorting block 55, #reads = 1085518 mem = 536880693 peak = 1122066432
[M::mem_process_seqs] Processed 702890 reads in 183.971 CPU sec, 6.042 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 291719, 21, 73)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 247, 700)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1704)
[M::mem_pestat] mean and std.dev: (358.98, 329.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2206)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 243, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (268.03, 133.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 930)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (101, 379, 1157)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3269)
[M::mem_pestat] mean and std.dev: (559.67, 469.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4325)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 306, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (294.59, 132.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 698652 sequences (100000176 bp) in 2.295 CPU sec, 2.420 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 291573, 34, 74)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (199, 281, 1100)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2902)
[M::mem_pestat] mean and std.dev: (544.25, 629.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3803)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 241, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (266.38, 133.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (291, 635, 1833)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4917)
[M::mem_pestat] mean and std.dev: (929.85, 922.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6459)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 309, 666)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1618)
[M::mem_pestat] mean and std.dev: (398.84, 302.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2094)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698650 reads in 146.653 CPU sec, 4.653 real sec
sorting block 56, #reads = 1085667 mem = 536881454 peak = 1122066432
[M::mem_process_seqs] Processed 698916 reads in 85.474 CPU sec, 2.693 real sec
[M::process] read 697656 sequences (100000204 bp) in 1.785 CPU sec, 1.866 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 292299, 33, 93)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 298, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1163)
[M::mem_pestat] mean and std.dev: (312.18, 219.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1501)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 242, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734)
[M::mem_pestat] mean and std.dev: (267.48, 134.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (280, 704, 1390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3610)
[M::mem_pestat] mean and std.dev: (830.94, 743.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4720)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 329, 656)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1584)
[M::mem_pestat] mean and std.dev: (378.36, 272.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2048)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 57, #reads = 1085069 mem = 536878038 peak = 1122066432
[M::process] read 697370 sequences (100000255 bp) in 2.058 CPU sec, 2.200 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 291755, 28, 75)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 277, 577)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1359)
[M::mem_pestat] mean and std.dev: (328.41, 235.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1750)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 244, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (269.70, 135.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (205, 607, 764)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1882)
[M::mem_pestat] mean and std.dev: (530.73, 416.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2441)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (297, 427, 1047)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2547)
[M::mem_pestat] mean and std.dev: (554.26, 466.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3297)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 373858 sequences (53561665 bp) in 0.970 CPU sec, 0.970 real sec...
[M::mem_process_seqs] Processed 698652 reads in 131.443 CPU sec, 4.187 real sec
[M::mem_process_seqs] Processed 697656 reads in 83.731 CPU sec, 2.650 real sec
sorting block 58, #reads = 1086537 mem = 536878177 peak = 1122066432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 290806, 41, 58)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 321, 797)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2079)
[M::mem_pestat] mean and std.dev: (483.10, 501.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2720)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 244, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (270.66, 136.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (124, 581, 1687)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4813)
[M::mem_pestat] mean and std.dev: (806.13, 791.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6376)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (309, 396, 537)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (377.27, 177.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 156224, 9, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 236, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (274.03, 173.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 242, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (269.24, 136.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 304, 608)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1434)
[M::mem_pestat] mean and std.dev: (396.29, 282.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1847)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697370 reads in 101.958 CPU sec, 3.307 real sec
[M::mem_process_seqs] Processed 373858 reads in 50.061 CPU sec, 1.615 real sec
sorting block 59, #reads = 1085747 mem = 536879618 peak = 1122066432
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 65221546
overall: 69995233280 mem 7883.401 user 266.578 sys 1471.097 real
sorting block 60, #reads = 551345 mem = 273020693 peak = 1122066432
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_27F-T1-TDNA-1_A23MHWWLT4_1.L008\tPU:L008\tSM:1136_27F-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_27F-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0001.1136_27F-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0001.1136_27F-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 1471.141 sec; CPU: 8149.989 sec
uniq header count 1
merging 61 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3684623724
output reads: 65768827
bam_mem_sort: 61 calls 24.527 user 0.192 sys 24.817 real
bam_write: 61 calls 24.957 user 0.780 sys 26.067 real
execute: 1 calls 12.324 user 0.661 sys 1490.722 real
merge_files: 1 calls 12.313 user 0.632 sys 18.942 real
parse_chunk: 969 calls 33.350 user 8.874 sys 42.943 real
read_chunk: 2618 calls 24.341 user 21.510 sys 222.429 real
sort_block: 1 calls 19.128 user 21.003 sys 214.403 real
write_chunk: 1647 calls 11.998 user 0.465 sys 18.275 real
overall: 2793758720 mem 739.892 user 38.667 sys 1490.745 real
ls: cannot access '*.cram': No such file or directory