This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_4GR-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2698.431 CPU sec, 95.814 real sec [M::process] read 700944 sequences (100000152 bp) in 1.053 CPU sec, 1.057 real sec... [M::process] read 699546 sequences (100000275 bp) in 1.673 CPU sec, 1.701 real sec... [M::process] read 699240 sequences (100000177 bp) in 1.654 CPU sec, 1.712 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 285387, 28, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 268, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1259) [M::mem_pestat] mean and std.dev: (296.55, 222.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 232, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (260.44, 132.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (133, 432, 1157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3205) [M::mem_pestat] mean and std.dev: (498.41, 439.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4229) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 369, 915) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2323) [M::mem_pestat] mean and std.dev: (564.24, 528.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3027) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698786 sequences (100000201 bp) in 1.639 CPU sec, 1.688 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 284887, 32, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 302, 1437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3913) [M::mem_pestat] mean and std.dev: (715.76, 705.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5151) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 236, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (264.01, 134.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (476, 704, 2230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5738) [M::mem_pestat] mean and std.dev: (1178.00, 1019.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7492) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 293, 573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1373) [M::mem_pestat] mean and std.dev: (359.78, 206.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1773) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 283962, 22, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 435, 601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1337) [M::mem_pestat] mean and std.dev: (397.35, 254.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1705) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 238, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (265.86, 135.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (112, 614, 2253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6535) [M::mem_pestat] mean and std.dev: (1104.45, 1151.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8676) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 335, 951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2477) [M::mem_pestat] mean and std.dev: (600.36, 583.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3240) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700944 reads in 313.998 CPU sec, 10.197 real sec [M::mem_process_seqs] Processed 699546 reads in 208.618 CPU sec, 6.832 real sec [M::mem_process_seqs] Processed 699240 reads in 230.293 CPU sec, 7.538 real sec sorting block 0, #reads = 1078882 mem = 536881334 peak = 579788800 [M::process] read 697700 sequences (100000066 bp) in 2.689 CPU sec, 2.876 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 284543, 31, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (238, 481, 1911) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5257) [M::mem_pestat] mean and std.dev: (976.06, 893.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6930) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 241, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (268.57, 136.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (87, 658, 1136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3234) [M::mem_pestat] mean and std.dev: (753.61, 794.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4283) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 313, 1205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3195) [M::mem_pestat] mean and std.dev: (640.15, 727.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4190) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697138 sequences (100000136 bp) in 2.416 CPU sec, 2.517 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 283892, 19, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 722, 1363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3637) [M::mem_pestat] mean and std.dev: (781.50, 658.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4774) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 244, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (270.89, 137.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 182, 1264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3752) [M::mem_pestat] mean and std.dev: (469.59, 597.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4996) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 260, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (296.09, 193.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698786 reads in 189.890 CPU sec, 6.040 real sec sorting block 1, #reads = 1078156 mem = 536879941 peak = 762241024 [M::process] read 696742 sequences (100000155 bp) in 1.743 CPU sec, 1.784 real sec... [M::mem_process_seqs] Processed 697700 reads in 126.926 CPU sec, 4.010 real sec sorting block 2, #reads = 1077050 mem = 536878538 peak = 865079296 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 283887, 14, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 400, 904) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2342) [M::mem_pestat] mean and std.dev: (559.68, 491.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3061) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 244, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (271.31, 137.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (363, 517, 985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2229) [M::mem_pestat] mean and std.dev: (583.50, 358.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2851) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 301, 1164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3044) [M::mem_pestat] mean and std.dev: (573.35, 651.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3984) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695652 sequences (100000237 bp) in 2.536 CPU sec, 2.758 real sec... [M::process] read 695688 sequences (100000206 bp) in 1.749 CPU sec, 1.766 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283549, 16, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 439, 743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1873) [M::mem_pestat] mean and std.dev: (491.05, 361.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 246, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (274.31, 139.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (359, 585, 1152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2738) [M::mem_pestat] mean and std.dev: (666.56, 464.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3531) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (249, 370, 748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1746) [M::mem_pestat] mean and std.dev: (519.67, 383.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2245) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697138 reads in 190.599 CPU sec, 6.169 real sec [M::mem_process_seqs] Processed 696742 reads in 125.796 CPU sec, 4.012 real sec sorting block 3, #reads = 1076263 mem = 536879615 peak = 865079296 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 282903, 22, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 473, 732) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1876) [M::mem_pestat] mean and std.dev: (476.68, 388.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 250, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (277.12, 140.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (180, 679, 1402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3846) [M::mem_pestat] mean and std.dev: (850.27, 714.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5068) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 176, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963) [M::mem_pestat] mean and std.dev: (243.14, 134.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1230) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695760 sequences (100000143 bp) in 2.320 CPU sec, 2.433 real sec... [M::process] read 695600 sequences (100000301 bp) in 1.821 CPU sec, 1.825 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282798, 26, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (542, 731, 2214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5558) [M::mem_pestat] mean and std.dev: (1403.46, 1394.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7230) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (275.92, 139.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (153, 375, 857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2265) [M::mem_pestat] mean and std.dev: (490.39, 453.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2969) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (256, 471, 1134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2890) [M::mem_pestat] mean and std.dev: (577.72, 442.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3768) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695652 reads in 192.509 CPU sec, 6.211 real sec [M::mem_process_seqs] Processed 695688 reads in 128.909 CPU sec, 4.116 real sec sorting block 4, #reads = 1075338 mem = 536880287 peak = 865079296 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 283153, 16, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 284, 616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1476) [M::mem_pestat] mean and std.dev: (343.15, 290.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1906) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (276.26, 139.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (93, 371, 1829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5301) [M::mem_pestat] mean and std.dev: (428.00, 556.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7037) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (337, 773, 3889) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10993) [M::mem_pestat] mean and std.dev: (2456.28, 2999.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14545) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696132 sequences (100000292 bp) in 2.371 CPU sec, 2.495 real sec... [M::process] read 695358 sequences (100000036 bp) in 1.856 CPU sec, 1.860 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 283593, 24, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (247, 443, 782) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1852) [M::mem_pestat] mean and std.dev: (424.75, 274.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2387) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (277.42, 141.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (182, 541, 974) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2558) [M::mem_pestat] mean and std.dev: (461.29, 424.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3350) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 495, 1043) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2695) [M::mem_pestat] mean and std.dev: (805.68, 763.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3858) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695760 reads in 184.294 CPU sec, 5.916 real sec sorting block 5, #reads = 1075082 mem = 536882596 peak = 865079296 [M::mem_process_seqs] Processed 695600 reads in 122.704 CPU sec, 3.996 real sec sorting block 6, #reads = 1074450 mem = 536883080 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 283242, 16, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 795, 1147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3047) [M::mem_pestat] mean and std.dev: (651.30, 530.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3997) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 247, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (274.00, 138.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (300, 702, 1172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2916) [M::mem_pestat] mean and std.dev: (599.43, 469.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3788) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 237, 910) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2384) [M::mem_pestat] mean and std.dev: (499.71, 449.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3121) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695044 sequences (100000299 bp) in 2.549 CPU sec, 2.768 real sec... [M::process] read 694852 sequences (100000301 bp) in 1.962 CPU sec, 1.972 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282883, 26, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 668, 1073) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2827) [M::mem_pestat] mean and std.dev: (632.21, 488.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3704) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 251, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (278.04, 140.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (208, 521, 1554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4246) [M::mem_pestat] mean and std.dev: (856.36, 875.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5592) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 360, 601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1383) [M::mem_pestat] mean and std.dev: (366.86, 195.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1774) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696132 reads in 195.452 CPU sec, 6.301 real sec [M::mem_process_seqs] Processed 695358 reads in 131.299 CPU sec, 4.251 real sec sorting block 7, #reads = 1075581 mem = 536882310 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 282843, 23, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 356, 759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1865) [M::mem_pestat] mean and std.dev: (447.68, 407.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 250, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (277.18, 139.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (122, 310, 651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1709) [M::mem_pestat] mean and std.dev: (383.52, 358.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2238) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (359, 877, 1673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4301) [M::mem_pestat] mean and std.dev: (1021.35, 937.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5615) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694936 sequences (100000222 bp) in 2.588 CPU sec, 3.075 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 282868, 29, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 380, 992) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2574) [M::mem_pestat] mean and std.dev: (536.97, 528.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3365) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 251, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (276.69, 138.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (109, 395, 1569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4489) [M::mem_pestat] mean and std.dev: (912.62, 891.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5949) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 276, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012) [M::mem_pestat] mean and std.dev: (319.52, 186.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1299) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694892 sequences (100000289 bp) in 1.914 CPU sec, 1.916 real sec... [M::mem_process_seqs] Processed 695044 reads in 198.091 CPU sec, 6.447 real sec [M::mem_process_seqs] Processed 694852 reads in 130.030 CPU sec, 4.225 real sec sorting block 8, #reads = 1074695 mem = 536877791 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 282204, 23, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (250, 355, 1184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3052) [M::mem_pestat] mean and std.dev: (652.06, 588.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3986) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (274.58, 136.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (96, 367, 1137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3219) [M::mem_pestat] mean and std.dev: (713.26, 792.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4260) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 389, 973) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2495) [M::mem_pestat] mean and std.dev: (545.82, 482.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3256) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695140 sequences (100000060 bp) in 2.940 CPU sec, 3.244 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 282455, 21, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 338, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1122) [M::mem_pestat] mean and std.dev: (326.78, 183.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 250, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (275.93, 137.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (251, 551, 2025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5573) [M::mem_pestat] mean and std.dev: (1057.30, 1359.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7347) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (280, 572, 894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2122) [M::mem_pestat] mean and std.dev: (667.48, 568.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2942) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695870 sequences (100000231 bp) in 1.954 CPU sec, 1.956 real sec... [M::mem_process_seqs] Processed 694936 reads in 206.896 CPU sec, 6.712 real sec sorting block 9, #reads = 1075926 mem = 536879086 peak = 897064960 [M::mem_process_seqs] Processed 694892 reads in 130.726 CPU sec, 4.252 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 283270, 27, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (306, 489, 1188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2952) [M::mem_pestat] mean and std.dev: (678.76, 496.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3834) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (275.49, 138.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (513, 896, 2290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5844) [M::mem_pestat] mean and std.dev: (1475.07, 1331.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7621) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 266, 642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1522) [M::mem_pestat] mean and std.dev: (275.16, 146.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1962) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695458 sequences (100000253 bp) in 2.823 CPU sec, 3.132 real sec... sorting block 10, #reads = 1074296 mem = 536887383 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 283092, 8, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (243, 473, 862) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2100) [M::mem_pestat] mean and std.dev: (614.78, 438.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2719) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 247, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (273.04, 136.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (260, 505, 1053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2639) [M::mem_pestat] mean and std.dev: (659.03, 534.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3432) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 695514 sequences (100000120 bp) in 2.157 CPU sec, 2.188 real sec... [M::mem_process_seqs] Processed 695140 reads in 208.215 CPU sec, 6.754 real sec [M::mem_process_seqs] Processed 695870 reads in 133.989 CPU sec, 4.329 real sec sorting block 11, #reads = 1074295 mem = 536882673 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 282830, 24, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (270, 430, 1967) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5361) [M::mem_pestat] mean and std.dev: (1155.71, 1159.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7058) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (274.53, 137.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (327, 1373, 3970) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11256) [M::mem_pestat] mean and std.dev: (2228.33, 2616.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14899) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 395, 1191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3095) [M::mem_pestat] mean and std.dev: (643.96, 612.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4047) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695304 sequences (100000183 bp) in 2.775 CPU sec, 2.993 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282044, 24, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 456, 1505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4149) [M::mem_pestat] mean and std.dev: (752.00, 724.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 695310 sequences (100000093 bp) in 2.045 CPU sec, 2.059 real sec... [M::mem_pestat] (25, 50, 75) percentile: (166, 248, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (273.56, 136.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (409, 622, 816) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1630) [M::mem_pestat] mean and std.dev: (505.29, 349.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2037) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (266, 336, 643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1397) [M::mem_pestat] mean and std.dev: (415.10, 242.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1774) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695458 reads in 203.310 CPU sec, 6.574 real sec [M::mem_process_seqs] Processed 695514 reads in 135.087 CPU sec, 4.366 real sec sorting block 12, #reads = 1073743 mem = 536877488 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282629, 20, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (329, 681, 1670) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4352) [M::mem_pestat] mean and std.dev: (875.06, 674.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (274.85, 137.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (360, 1219, 2272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6096) [M::mem_pestat] mean and std.dev: (1086.50, 886.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8008) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 324, 657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1587) [M::mem_pestat] mean and std.dev: (415.59, 318.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2052) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694938 sequences (100000059 bp) in 2.766 CPU sec, 2.976 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 282516, 39, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 535, 1030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2800) [M::mem_pestat] mean and std.dev: (671.00, 622.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (276.32, 139.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (97, 247, 709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1933) [M::mem_pestat] mean and std.dev: (466.65, 530.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2589) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 319, 1047) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2767) [M::mem_pestat] mean and std.dev: (587.31, 654.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3627) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698914 sequences (100000096 bp) in 2.069 CPU sec, 2.078 real sec... [M::mem_process_seqs] Processed 695304 reads in 202.297 CPU sec, 6.519 real sec [M::mem_process_seqs] Processed 695310 reads in 137.243 CPU sec, 4.442 real sec sorting block 13, #reads = 1072087 mem = 536881313 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282443, 23, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (287, 495, 2654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7388) [M::mem_pestat] mean and std.dev: (971.03, 1190.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 251, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (278.64, 141.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 340, 659) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1881) [M::mem_pestat] mean and std.dev: (427.32, 471.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2492) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 292, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (306.70, 163.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 14, #reads = 1073224 mem = 536881406 peak = 897064960 [M::process] read 700156 sequences (100000081 bp) in 2.812 CPU sec, 3.025 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 284377, 31, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (222, 429, 815) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2001) [M::mem_pestat] mean and std.dev: (434.33, 283.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 239, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (266.80, 135.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (212, 387, 935) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2381) [M::mem_pestat] mean and std.dev: (578.90, 503.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3104) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 336, 1158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3084) [M::mem_pestat] mean and std.dev: (529.97, 467.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4047) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698486 sequences (100000035 bp) in 2.094 CPU sec, 2.102 real sec... [M::mem_process_seqs] Processed 694938 reads in 199.979 CPU sec, 6.463 real sec [M::mem_process_seqs] Processed 698914 reads in 132.751 CPU sec, 4.219 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 284103, 21, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 393, 1094) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2904) [M::mem_pestat] mean and std.dev: (524.75, 512.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 236, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (264.46, 134.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (152, 269, 1599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4493) [M::mem_pestat] mean and std.dev: (772.10, 944.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5940) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 314, 1038) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2672) [M::mem_pestat] mean and std.dev: (453.20, 410.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3489) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 15, #reads = 1074856 mem = 536877938 peak = 897064960 [M::process] read 697850 sequences (100000130 bp) in 2.722 CPU sec, 2.907 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 284059, 16, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 205, 557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1431) [M::mem_pestat] mean and std.dev: (337.96, 330.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1868) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 240, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (266.74, 134.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (266, 579, 2665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7463) [M::mem_pestat] mean and std.dev: (1553.75, 2025.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9862) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 358, 1292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3510) [M::mem_pestat] mean and std.dev: (663.86, 711.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4619) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697256 sequences (100000145 bp) in 1.864 CPU sec, 1.872 real sec... [M::mem_process_seqs] Processed 700156 reads in 187.505 CPU sec, 5.994 real sec [M::mem_process_seqs] Processed 698486 reads in 124.844 CPU sec, 3.973 real sec sorting block 16, #reads = 1079187 mem = 536880944 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 283817, 25, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 387, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (348.43, 174.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1175) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 243, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (268.34, 134.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (102, 359, 921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2559) [M::mem_pestat] mean and std.dev: (497.33, 577.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3378) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (238, 289, 2483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6973) [M::mem_pestat] mean and std.dev: (1246.68, 1721.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9218) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697178 sequences (100000184 bp) in 2.845 CPU sec, 3.113 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 284095, 22, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (244, 842, 1221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3175) [M::mem_pestat] mean and std.dev: (789.06, 644.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4152) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 244, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (270.17, 135.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (236, 1032, 2280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6368) [M::mem_pestat] mean and std.dev: (1352.95, 1242.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8412) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (299, 494, 964) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2294) [M::mem_pestat] mean and std.dev: (587.92, 459.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2959) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696962 sequences (100000101 bp) in 2.021 CPU sec, 2.029 real sec... [M::mem_process_seqs] Processed 697850 reads in 192.408 CPU sec, 6.150 real sec [M::mem_process_seqs] Processed 697256 reads in 128.524 CPU sec, 4.088 real sec sorting block 17, #reads = 1079847 mem = 536879813 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 284274, 15, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 338, 1146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3064) [M::mem_pestat] mean and std.dev: (545.17, 533.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4023) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (270.90, 135.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (127, 548, 975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2671) [M::mem_pestat] mean and std.dev: (526.00, 462.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3519) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 275, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (304.34, 123.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1064) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696436 sequences (100000161 bp) in 2.995 CPU sec, 3.195 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 283980, 19, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 282, 737) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1841) [M::mem_pestat] mean and std.dev: (561.05, 551.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2769) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (272.86, 137.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (158, 835, 1950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5534) [M::mem_pestat] mean and std.dev: (1503.16, 1765.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8564) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 331, 1550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4218) [M::mem_pestat] mean and std.dev: (859.88, 1086.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5552) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697178 reads in 190.695 CPU sec, 6.095 real sec [M::process] read 696558 sequences (100000019 bp) in 1.922 CPU sec, 1.928 real sec... sorting block 18, #reads = 1079278 mem = 536878875 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 283406, 12, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 507, 585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1425) [M::mem_pestat] mean and std.dev: (295.10, 175.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1845) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 248, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (274.57, 138.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (245, 823, 1473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3929) [M::mem_pestat] mean and std.dev: (835.82, 781.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5157) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (250, 341, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952) [M::mem_pestat] mean and std.dev: (344.81, 136.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1186) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695948 sequences (100000297 bp) in 2.202 CPU sec, 2.208 real sec... [M::mem_process_seqs] Processed 696436 reads in 111.188 CPU sec, 3.752 real sec sorting block 19, #reads = 1077721 mem = 536878937 peak = 897064960 [M::mem_process_seqs] Processed 696962 reads in 283.502 CPU sec, 9.249 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 283731, 29, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 377, 1540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4216) [M::mem_pestat] mean and std.dev: (870.52, 999.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 249, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (275.57, 139.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (505, 887, 1735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4195) [M::mem_pestat] mean and std.dev: (1245.30, 1136.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5792) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 291, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1059) [M::mem_pestat] mean and std.dev: (318.56, 170.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1349) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695482 sequences (100000085 bp) in 2.836 CPU sec, 3.027 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282912, 18, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 290, 812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2064) [M::mem_pestat] mean and std.dev: (350.93, 264.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (275.17, 138.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (177, 498, 1282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3492) [M::mem_pestat] mean and std.dev: (605.17, 531.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4597) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 420, 1228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3252) [M::mem_pestat] mean and std.dev: (560.58, 531.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4264) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696558 reads in 212.474 CPU sec, 6.790 real sec sorting block 20, #reads = 1077842 mem = 536877955 peak = 897064960 [M::process] read 695810 sequences (100000096 bp) in 1.855 CPU sec, 1.926 real sec... [M::mem_process_seqs] Processed 695948 reads in 129.950 CPU sec, 4.147 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 283075, 16, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 287, 1621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4485) [M::mem_pestat] mean and std.dev: (636.75, 624.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 251, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (277.98, 140.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (87, 275, 1566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4524) [M::mem_pestat] mean and std.dev: (961.50, 1300.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6163) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 281, 932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2422) [M::mem_pestat] mean and std.dev: (513.17, 378.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3167) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 21, #reads = 1077165 mem = 536880024 peak = 897064960 [M::process] read 695012 sequences (100000031 bp) in 2.950 CPU sec, 3.192 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 283530, 23, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 352, 670) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1682) [M::mem_pestat] mean and std.dev: (486.14, 417.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2188) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 250, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (276.82, 139.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (311, 585, 1967) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5279) [M::mem_pestat] mean and std.dev: (902.00, 809.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6935) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (277, 452, 1652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4402) [M::mem_pestat] mean and std.dev: (911.86, 1075.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5777) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695052 sequences (100000010 bp) in 1.975 CPU sec, 1.978 real sec... [M::mem_process_seqs] Processed 695482 reads in 200.672 CPU sec, 6.454 real sec [M::mem_process_seqs] Processed 695810 reads in 133.535 CPU sec, 4.262 real sec sorting block 22, #reads = 1076068 mem = 536878898 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 282992, 31, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 298, 881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2153) [M::mem_pestat] mean and std.dev: (581.95, 516.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (277.78, 139.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (100, 282, 811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2233) [M::mem_pestat] mean and std.dev: (455.21, 455.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2944) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 382, 639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1555) [M::mem_pestat] mean and std.dev: (428.21, 291.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2013) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695134 sequences (100000018 bp) in 2.831 CPU sec, 3.059 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 283062, 34, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 360, 613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1435) [M::mem_pestat] mean and std.dev: (373.20, 233.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1846) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 250, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (275.66, 137.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (505, 1280, 1414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3232) [M::mem_pestat] mean and std.dev: (977.30, 605.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4141) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 395, 1463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3909) [M::mem_pestat] mean and std.dev: (775.03, 754.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5132) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694678 sequences (100000003 bp) in 2.155 CPU sec, 2.159 real sec... [M::mem_process_seqs] Processed 695012 reads in 199.814 CPU sec, 6.398 real sec [M::mem_process_seqs] Processed 695052 reads in 132.861 CPU sec, 4.217 real sec sorting block 23, #reads = 1076356 mem = 536880532 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 282735, 29, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 295, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1214) [M::mem_pestat] mean and std.dev: (343.98, 217.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 251, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (278.23, 140.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (275, 902, 2397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6641) [M::mem_pestat] mean and std.dev: (1232.15, 1136.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8763) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 425, 605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1383) [M::mem_pestat] mean and std.dev: (368.65, 206.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1772) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695068 sequences (100000151 bp) in 2.867 CPU sec, 3.110 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282653, 20, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (406, 887, 2824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7660) [M::mem_pestat] mean and std.dev: (1507.56, 1914.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10078) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (277.38, 138.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (185, 269, 979) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2567) [M::mem_pestat] mean and std.dev: (400.11, 391.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3361) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 300, 739) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1803) [M::mem_pestat] mean and std.dev: (421.62, 386.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2335) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695402 sequences (100000068 bp) in 1.882 CPU sec, 1.889 real sec... [M::mem_process_seqs] Processed 695134 reads in 200.086 CPU sec, 6.474 real sec sorting block 24, #reads = 1075905 mem = 536880797 peak = 897064960 [M::mem_process_seqs] Processed 694678 reads in 132.690 CPU sec, 4.262 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 282768, 18, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (276, 426, 1045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2583) [M::mem_pestat] mean and std.dev: (625.45, 507.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3352) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 251, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (277.76, 139.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (168, 586, 1181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3207) [M::mem_pestat] mean and std.dev: (682.81, 652.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4220) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 491, 1822) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5104) [M::mem_pestat] mean and std.dev: (869.70, 883.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6745) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 25, #reads = 1074149 mem = 536877106 peak = 897064960 [M::process] read 694920 sequences (100000068 bp) in 3.125 CPU sec, 3.471 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 283261, 18, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 347, 549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1281) [M::mem_pestat] mean and std.dev: (393.93, 306.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (274.01, 137.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (249, 506, 1629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4389) [M::mem_pestat] mean and std.dev: (852.33, 803.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5769) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 259, 668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1612) [M::mem_pestat] mean and std.dev: (390.49, 349.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2084) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695068 reads in 202.516 CPU sec, 6.517 real sec [M::process] read 695570 sequences (100000062 bp) in 2.200 CPU sec, 2.231 real sec... [M::mem_process_seqs] Processed 695402 reads in 139.069 CPU sec, 4.408 real sec sorting block 26, #reads = 1074292 mem = 536882623 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 282674, 25, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 383, 936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2366) [M::mem_pestat] mean and std.dev: (522.54, 453.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3081) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 250, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (276.26, 138.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (510, 1305, 5051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14133) [M::mem_pestat] mean and std.dev: (2335.56, 2152.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18674) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 282, 904) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2320) [M::mem_pestat] mean and std.dev: (531.04, 494.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3028) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695294 sequences (100000116 bp) in 2.902 CPU sec, 3.129 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282951, 30, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 325, 706) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1796) [M::mem_pestat] mean and std.dev: (386.46, 412.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2341) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (274.64, 137.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (199, 767, 2933) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8401) [M::mem_pestat] mean and std.dev: (1604.83, 1947.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11135) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (247, 636, 1535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4111) [M::mem_pestat] mean and std.dev: (774.04, 665.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5399) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695164 sequences (100000033 bp) in 2.078 CPU sec, 2.079 real sec... [M::mem_process_seqs] Processed 694920 reads in 206.271 CPU sec, 6.663 real sec [M::mem_process_seqs] Processed 695570 reads in 135.868 CPU sec, 4.335 real sec sorting block 27, #reads = 1073590 mem = 536881612 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282840, 40, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 430, 1998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5556) [M::mem_pestat] mean and std.dev: (861.52, 1001.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7335) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (275.50, 138.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (446, 980, 1829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4595) [M::mem_pestat] mean and std.dev: (982.69, 797.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5978) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 243, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (280.25, 152.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695224 sequences (100000021 bp) in 3.325 CPU sec, 3.625 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282836, 33, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 241, 738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1870) [M::mem_pestat] mean and std.dev: (475.29, 444.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (274.56, 137.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (529, 1190, 2485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6397) [M::mem_pestat] mean and std.dev: (1396.52, 1096.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8353) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (246, 465, 1463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3897) [M::mem_pestat] mean and std.dev: (678.66, 660.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5114) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695294 reads in 197.094 CPU sec, 6.338 real sec [M::process] read 695610 sequences (100000204 bp) in 2.189 CPU sec, 2.237 real sec... [M::mem_process_seqs] Processed 695164 reads in 134.890 CPU sec, 4.443 real sec sorting block 28, #reads = 1074869 mem = 536878248 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 283178, 22, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 261, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989) [M::mem_pestat] mean and std.dev: (324.44, 174.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1247) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (275.17, 138.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (178, 289, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1132) [M::mem_pestat] mean and std.dev: (287.95, 187.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1450) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 294, 610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1474) [M::mem_pestat] mean and std.dev: (287.65, 152.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1906) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 29, #reads = 1073565 mem = 536883963 peak = 897064960 [M::process] read 695780 sequences (100000176 bp) in 3.377 CPU sec, 3.591 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 282896, 30, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 460, 1035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2707) [M::mem_pestat] mean and std.dev: (644.38, 542.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (275.80, 140.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (120, 262, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1419) [M::mem_pestat] mean and std.dev: (315.86, 298.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1852) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 403, 1371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3741) [M::mem_pestat] mean and std.dev: (854.54, 902.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4926) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695224 reads in 195.484 CPU sec, 6.408 real sec [M::process] read 698882 sequences (100000026 bp) in 2.049 CPU sec, 2.071 real sec... [M::mem_process_seqs] Processed 695610 reads in 135.893 CPU sec, 4.306 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282198, 40, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 253, 588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1436) [M::mem_pestat] mean and std.dev: (323.36, 275.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1860) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (276.59, 141.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (223, 839, 1726) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4732) [M::mem_pestat] mean and std.dev: (916.17, 1080.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6235) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (328, 385, 689) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1411) [M::mem_pestat] mean and std.dev: (376.82, 165.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1772) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 30, #reads = 1073611 mem = 536878865 peak = 897064960 [M::process] read 699100 sequences (100000111 bp) in 3.205 CPU sec, 3.451 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 283987, 15, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 322, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (287.55, 105.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1009) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 239, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (265.72, 134.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (436, 787, 1845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4663) [M::mem_pestat] mean and std.dev: (1048.29, 754.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6072) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 316, 1018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2708) [M::mem_pestat] mean and std.dev: (581.43, 623.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3553) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695780 reads in 198.584 CPU sec, 6.360 real sec [M::process] read 698204 sequences (100000044 bp) in 1.911 CPU sec, 1.925 real sec... [M::mem_process_seqs] Processed 698882 reads in 133.335 CPU sec, 4.235 real sec sorting block 31, #reads = 1073136 mem = 536883075 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 284634, 35, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 704, 1529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4191) [M::mem_pestat] mean and std.dev: (911.27, 751.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 239, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (264.37, 132.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (307, 1043, 2295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6271) [M::mem_pestat] mean and std.dev: (1518.03, 1484.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8259) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 398, 980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2484) [M::mem_pestat] mean and std.dev: (492.17, 374.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3236) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697928 sequences (100000053 bp) in 3.160 CPU sec, 3.412 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 284056, 22, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 447, 1150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2948) [M::mem_pestat] mean and std.dev: (595.53, 497.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3847) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 242, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (267.68, 134.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (90, 605, 965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2715) [M::mem_pestat] mean and std.dev: (530.37, 467.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3590) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 345, 2060) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5722) [M::mem_pestat] mean and std.dev: (1165.88, 1423.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7553) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699100 reads in 191.117 CPU sec, 6.143 real sec [M::mem_process_seqs] Processed 698204 reads in 129.160 CPU sec, 4.089 real sec [M::process] read 697246 sequences (100000275 bp) in 2.179 CPU sec, 2.246 real sec... sorting block 32, #reads = 1077687 mem = 536877588 peak = 897064960 [M::process] read 696890 sequences (100000180 bp) in 3.285 CPU sec, 3.637 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 283565, 20, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 579, 1306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3506) [M::mem_pestat] mean and std.dev: (734.11, 760.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (271.09, 136.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (583, 674, 1445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3169) [M::mem_pestat] mean and std.dev: (641.62, 384.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4031) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 315, 941) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2477) [M::mem_pestat] mean and std.dev: (485.53, 472.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3245) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697246 reads in 111.453 CPU sec, 3.516 real sec sorting block 33, #reads = 1078044 mem = 536877636 peak = 897064960 [M::process] read 696320 sequences (100000072 bp) in 2.148 CPU sec, 2.224 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 284275, 23, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 382, 798) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2012) [M::mem_pestat] mean and std.dev: (450.19, 363.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 242, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (268.50, 135.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (317, 741, 1437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3677) [M::mem_pestat] mean and std.dev: (804.36, 598.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4797) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (299, 515, 1332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3398) [M::mem_pestat] mean and std.dev: (762.94, 651.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4431) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 283765, 32, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (271, 449, 1273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3277) [M::mem_pestat] mean and std.dev: (640.56, 496.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4279) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 246, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (272.55, 137.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (147, 566, 1660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4686) [M::mem_pestat] mean and std.dev: (857.39, 795.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6199) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 393, 1078) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2860) [M::mem_pestat] mean and std.dev: (676.91, 562.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3751) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697928 reads in 296.543 CPU sec, 9.457 real sec [M::process] read 696372 sequences (100000087 bp) in 2.476 CPU sec, 2.526 real sec... [M::mem_process_seqs] Processed 696890 reads in 152.240 CPU sec, 4.831 real sec sorting block 34, #reads = 1076254 mem = 536879458 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 283760, 13, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 267, 863) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2199) [M::mem_pestat] mean and std.dev: (529.00, 504.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2867) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (274.07, 137.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (310, 454, 2420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6640) [M::mem_pestat] mean and std.dev: (1063.15, 969.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8750) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 314, 1236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3270) [M::mem_pestat] mean and std.dev: (756.08, 899.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4354) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695876 sequences (100000097 bp) in 3.096 CPU sec, 3.362 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 283627, 32, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 202, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (254.86, 147.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 248, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (275.03, 139.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (324, 1330, 1967) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5253) [M::mem_pestat] mean and std.dev: (1286.12, 1145.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6896) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 509, 1261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3347) [M::mem_pestat] mean and std.dev: (792.31, 657.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4390) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696320 reads in 191.635 CPU sec, 6.140 real sec [M::process] read 695176 sequences (100000214 bp) in 1.993 CPU sec, 2.026 real sec... sorting block 35, #reads = 1076396 mem = 536880357 peak = 897064960 [M::mem_process_seqs] Processed 696372 reads in 128.235 CPU sec, 4.100 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 283237, 31, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (259, 517, 1545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4117) [M::mem_pestat] mean and std.dev: (771.36, 815.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (277.10, 141.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (262, 820, 1754) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4738) [M::mem_pestat] mean and std.dev: (965.22, 1131.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6230) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (249, 622, 1651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4455) [M::mem_pestat] mean and std.dev: (936.73, 979.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5857) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 36, #reads = 1078036 mem = 536877470 peak = 897064960 [M::process] read 694838 sequences (100000236 bp) in 2.976 CPU sec, 3.250 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282981, 20, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 326, 1151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3093) [M::mem_pestat] mean and std.dev: (623.76, 612.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4064) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 251, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (277.69, 139.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (261, 1639, 2323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6447) [M::mem_pestat] mean and std.dev: (1293.20, 936.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8509) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 377, 2136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6070) [M::mem_pestat] mean and std.dev: (848.93, 1046.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8037) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694858 sequences (100000135 bp) in 2.026 CPU sec, 2.035 real sec... [M::mem_process_seqs] Processed 695876 reads in 196.678 CPU sec, 6.298 real sec [M::mem_process_seqs] Processed 695176 reads in 131.929 CPU sec, 4.182 real sec sorting block 37, #reads = 1077194 mem = 536882606 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 283203, 18, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (301, 406, 1027) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2479) [M::mem_pestat] mean and std.dev: (533.29, 358.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3205) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (279.22, 141.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (355, 991, 1354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3352) [M::mem_pestat] mean and std.dev: (812.00, 539.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4351) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (253, 340, 1002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2500) [M::mem_pestat] mean and std.dev: (500.83, 451.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3249) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694628 sequences (100000156 bp) in 2.849 CPU sec, 3.028 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 282747, 31, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (523, 637, 1024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2026) [M::mem_pestat] mean and std.dev: (671.89, 399.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 254, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (279.15, 140.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (208, 481, 1488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4048) [M::mem_pestat] mean and std.dev: (676.14, 734.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5328) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (443, 846, 1322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3080) [M::mem_pestat] mean and std.dev: (791.07, 612.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3959) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694838 reads in 191.848 CPU sec, 6.174 real sec [M::process] read 693708 sequences (100000225 bp) in 2.024 CPU sec, 2.045 real sec... [M::mem_process_seqs] Processed 694858 reads in 131.933 CPU sec, 4.229 real sec sorting block 38, #reads = 1076442 mem = 536882004 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 282565, 13, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 400, 798) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1970) [M::mem_pestat] mean and std.dev: (476.25, 413.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 254, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (280.43, 141.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (306, 697, 2497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6879) [M::mem_pestat] mean and std.dev: (1132.92, 973.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9070) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (335, 563, 2123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5699) [M::mem_pestat] mean and std.dev: (717.65, 608.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7487) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695076 sequences (100000103 bp) in 2.956 CPU sec, 3.205 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282167, 34, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 267, 770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1944) [M::mem_pestat] mean and std.dev: (433.70, 455.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 254, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (279.52, 139.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (189, 851, 4192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12198) [M::mem_pestat] mean and std.dev: (1805.44, 2099.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16201) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 613, 1372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3630) [M::mem_pestat] mean and std.dev: (788.03, 656.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4759) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694628 reads in 194.010 CPU sec, 6.255 real sec [M::process] read 694672 sequences (100000059 bp) in 2.020 CPU sec, 2.041 real sec... sorting block 39, #reads = 1076065 mem = 536885965 peak = 897064960 [M::mem_process_seqs] Processed 693708 reads in 131.055 CPU sec, 4.375 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 282832, 22, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 320, 819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2097) [M::mem_pestat] mean and std.dev: (471.40, 401.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2736) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 251, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (276.80, 139.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (90, 290, 723) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1989) [M::mem_pestat] mean and std.dev: (389.43, 444.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2622) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 295, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (310.43, 128.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 40, #reads = 1074526 mem = 536880293 peak = 897064960 [M::process] read 694884 sequences (100000177 bp) in 2.903 CPU sec, 3.146 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 282820, 29, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (271, 569, 1291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3331) [M::mem_pestat] mean and std.dev: (728.86, 614.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4351) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 250, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (276.12, 138.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (235, 958, 1377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3661) [M::mem_pestat] mean and std.dev: (727.57, 515.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4803) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 492, 888) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2180) [M::mem_pestat] mean and std.dev: (586.68, 443.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2826) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695194 sequences (100000042 bp) in 2.094 CPU sec, 2.105 real sec... [M::mem_process_seqs] Processed 695076 reads in 202.722 CPU sec, 6.720 real sec [M::mem_process_seqs] Processed 694672 reads in 133.593 CPU sec, 4.326 real sec sorting block 41, #reads = 1075080 mem = 536878530 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 282708, 18, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (295, 393, 727) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1591) [M::mem_pestat] mean and std.dev: (387.43, 218.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2023) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 250, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (275.60, 138.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (952, 1345, 1699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3193) [M::mem_pestat] mean and std.dev: (1041.57, 529.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3940) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (379, 767, 1451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3595) [M::mem_pestat] mean and std.dev: (869.61, 738.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4667) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695362 sequences (100000259 bp) in 3.192 CPU sec, 3.566 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282548, 22, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 270, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1245) [M::mem_pestat] mean and std.dev: (353.04, 272.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 248, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (273.97, 137.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (272, 464, 2383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6605) [M::mem_pestat] mean and std.dev: (1573.86, 1790.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8736) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (276, 403, 1124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2820) [M::mem_pestat] mean and std.dev: (579.83, 480.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3668) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694884 reads in 200.437 CPU sec, 6.482 real sec [M::process] read 695180 sequences (100000135 bp) in 2.147 CPU sec, 2.171 real sec... [M::mem_process_seqs] Processed 695194 reads in 142.681 CPU sec, 4.548 real sec sorting block 42, #reads = 1074270 mem = 536882639 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 282625, 23, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (283, 471, 742) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1660) [M::mem_pestat] mean and std.dev: (443.03, 292.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2119) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 248, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (273.51, 136.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (432, 985, 2117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5487) [M::mem_pestat] mean and std.dev: (1180.17, 999.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7172) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 444, 736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1812) [M::mem_pestat] mean and std.dev: (428.46, 253.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2350) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695132 sequences (100000159 bp) in 2.916 CPU sec, 3.128 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 282739, 17, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 300, 660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1656) [M::mem_pestat] mean and std.dev: (445.27, 398.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2154) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 248, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (273.70, 136.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (205, 998, 1938) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5404) [M::mem_pestat] mean and std.dev: (1235.06, 1169.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7137) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 471, 1248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3348) [M::mem_pestat] mean and std.dev: (705.93, 644.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4398) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695308 sequences (100000146 bp) in 2.206 CPU sec, 2.211 real sec... [M::mem_process_seqs] Processed 695362 reads in 201.660 CPU sec, 6.444 real sec [M::mem_process_seqs] Processed 695180 reads in 138.673 CPU sec, 4.430 real sec sorting block 43, #reads = 1073865 mem = 536878945 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282704, 25, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (261, 423, 624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1350) [M::mem_pestat] mean and std.dev: (450.06, 284.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 248, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (272.64, 135.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (92, 321, 675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1841) [M::mem_pestat] mean and std.dev: (353.82, 386.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2424) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (367, 629, 954) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2128) [M::mem_pestat] mean and std.dev: (556.07, 329.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2715) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 44, #reads = 1074269 mem = 536878647 peak = 897064960 [M::process] read 695190 sequences (100000215 bp) in 3.267 CPU sec, 3.535 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282793, 23, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 257, 979) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2501) [M::mem_pestat] mean and std.dev: (455.37, 486.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3262) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 248, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (274.41, 137.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (333, 568, 1141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2757) [M::mem_pestat] mean and std.dev: (520.35, 384.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3565) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 333, 1113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2933) [M::mem_pestat] mean and std.dev: (539.04, 577.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3843) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695132 reads in 196.469 CPU sec, 6.289 real sec [M::process] read 695374 sequences (100000032 bp) in 1.979 CPU sec, 1.985 real sec... [M::mem_process_seqs] Processed 695308 reads in 135.075 CPU sec, 4.282 real sec sorting block 45, #reads = 1074892 mem = 536878810 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 283193, 18, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (234, 368, 963) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2421) [M::mem_pestat] mean and std.dev: (549.49, 425.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3150) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 250, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (276.46, 139.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (287, 532, 766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1724) [M::mem_pestat] mean and std.dev: (544.71, 385.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2203) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 309, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (285.93, 103.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695454 sequences (100000176 bp) in 3.080 CPU sec, 3.324 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283364, 32, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 313, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1104) [M::mem_pestat] mean and std.dev: (347.89, 212.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (276.72, 140.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (269, 909, 3527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10043) [M::mem_pestat] mean and std.dev: (1980.88, 2103.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13301) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 348, 808) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1974) [M::mem_pestat] mean and std.dev: (451.31, 362.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2557) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696992 sequences (100000176 bp) in 2.001 CPU sec, 2.007 real sec... [M::mem_process_seqs] Processed 695190 reads in 201.202 CPU sec, 6.612 real sec [M::mem_process_seqs] Processed 695374 reads in 138.005 CPU sec, 4.401 real sec sorting block 46, #reads = 1074689 mem = 536880621 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 282953, 33, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 416, 1183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3127) [M::mem_pestat] mean and std.dev: (741.06, 686.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4099) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 250, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (278.34, 142.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (202, 1058, 2000) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5596) [M::mem_pestat] mean and std.dev: (1201.73, 1048.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7394) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 344, 731) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1779) [M::mem_pestat] mean and std.dev: (475.97, 380.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2303) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699134 sequences (100000035 bp) in 3.387 CPU sec, 3.808 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 283830, 15, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 269, 864) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2236) [M::mem_pestat] mean and std.dev: (421.30, 376.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2922) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (271.79, 138.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (271, 1100, 2114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5800) [M::mem_pestat] mean and std.dev: (985.38, 780.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7643) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (358, 629, 991) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2257) [M::mem_pestat] mean and std.dev: (616.72, 418.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2890) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695454 reads in 201.309 CPU sec, 6.438 real sec [M::process] read 698390 sequences (100000032 bp) in 1.925 CPU sec, 1.951 real sec... [M::mem_process_seqs] Processed 696992 reads in 138.721 CPU sec, 4.599 real sec sorting block 47, #reads = 1074621 mem = 536878479 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 284348, 25, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (292, 717, 935) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2221) [M::mem_pestat] mean and std.dev: (546.68, 324.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 238, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (265.70, 135.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 424, 2690) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8016) [M::mem_pestat] mean and std.dev: (912.17, 1170.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10679) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 271, 756) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1926) [M::mem_pestat] mean and std.dev: (333.12, 252.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2511) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697592 sequences (100000087 bp) in 2.613 CPU sec, 3.016 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 284414, 17, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 439, 739) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1841) [M::mem_pestat] mean and std.dev: (480.70, 414.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2392) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 241, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (268.42, 136.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (643, 870, 2047) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4855) [M::mem_pestat] mean and std.dev: (1063.19, 670.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6259) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 342, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1112) [M::mem_pestat] mean and std.dev: (347.43, 213.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1407) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697056 sequences (100000259 bp) in 2.004 CPU sec, 2.012 real sec... [M::mem_process_seqs] Processed 699134 reads in 201.327 CPU sec, 6.655 real sec sorting block 48, #reads = 1074728 mem = 536878754 peak = 897064960 [M::mem_process_seqs] Processed 698390 reads in 129.597 CPU sec, 4.198 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 283799, 18, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 282, 826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2222) [M::mem_pestat] mean and std.dev: (376.68, 413.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2920) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 243, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (271.78, 138.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (136, 903, 1002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2734) [M::mem_pestat] mean and std.dev: (603.12, 445.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3600) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 264, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (302.67, 131.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 49, #reads = 1078851 mem = 536879346 peak = 897064960 [M::process] read 696452 sequences (100000074 bp) in 3.545 CPU sec, 3.779 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 283839, 23, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 271, 685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1689) [M::mem_pestat] mean and std.dev: (388.07, 302.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2191) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (272.89, 138.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (206, 552, 972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2504) [M::mem_pestat] mean and std.dev: (500.70, 428.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3270) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 286, 1122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3010) [M::mem_pestat] mean and std.dev: (647.56, 711.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3954) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697592 reads in 195.804 CPU sec, 6.325 real sec [M::process] read 696256 sequences (100000019 bp) in 2.163 CPU sec, 2.206 real sec... [M::mem_process_seqs] Processed 697056 reads in 133.253 CPU sec, 4.394 real sec sorting block 50, #reads = 1079229 mem = 536876406 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 283482, 18, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 483, 1035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2635) [M::mem_pestat] mean and std.dev: (578.65, 441.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (272.89, 137.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (204, 1607, 2106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5910) [M::mem_pestat] mean and std.dev: (1284.28, 849.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7812) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 327, 762) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1838) [M::mem_pestat] mean and std.dev: (439.90, 379.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2376) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696412 sequences (100000269 bp) in 2.973 CPU sec, 3.180 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 283546, 13, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (260, 330, 780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1820) [M::mem_pestat] mean and std.dev: (452.27, 363.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2340) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (276.12, 140.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (434, 482, 935) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1937) [M::mem_pestat] mean and std.dev: (468.92, 281.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2438) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 322, 714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1742) [M::mem_pestat] mean and std.dev: (410.87, 279.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2256) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695760 sequences (100000179 bp) in 1.969 CPU sec, 1.976 real sec... [M::mem_process_seqs] Processed 696452 reads in 198.371 CPU sec, 6.516 real sec [M::mem_process_seqs] Processed 696256 reads in 131.682 CPU sec, 4.196 real sec sorting block 51, #reads = 1076573 mem = 536882158 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 283770, 22, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 336, 1031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2691) [M::mem_pestat] mean and std.dev: (512.44, 490.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (276.59, 141.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (189, 636, 1164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3114) [M::mem_pestat] mean and std.dev: (696.81, 692.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4089) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 450, 903) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2357) [M::mem_pestat] mean and std.dev: (444.83, 373.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3084) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695596 sequences (100000017 bp) in 3.125 CPU sec, 3.364 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 283356, 24, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 442, 1274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3388) [M::mem_pestat] mean and std.dev: (700.26, 649.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 250, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (278.46, 141.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (142, 228, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018) [M::mem_pestat] mean and std.dev: (246.00, 140.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1310) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 284, 1708) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4768) [M::mem_pestat] mean and std.dev: (740.48, 865.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6298) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696412 reads in 188.819 CPU sec, 6.053 real sec [M::process] read 695146 sequences (100000257 bp) in 2.117 CPU sec, 2.181 real sec... sorting block 52, #reads = 1077721 mem = 536880466 peak = 897064960 [M::mem_process_seqs] Processed 695760 reads in 128.697 CPU sec, 4.083 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 283639, 30, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 387, 552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1270) [M::mem_pestat] mean and std.dev: (342.49, 235.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 252, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (279.70, 143.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (383, 546, 1211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2867) [M::mem_pestat] mean and std.dev: (658.56, 584.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3695) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (275, 585, 1133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2849) [M::mem_pestat] mean and std.dev: (714.80, 596.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3707) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 53, #reads = 1077742 mem = 536882631 peak = 897064960 [M::process] read 695136 sequences (100000276 bp) in 3.008 CPU sec, 3.258 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 282976, 19, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 525, 1110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3002) [M::mem_pestat] mean and std.dev: (630.33, 470.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3948) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 253, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (279.49, 141.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (298, 835, 2280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6244) [M::mem_pestat] mean and std.dev: (1426.50, 1502.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8226) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 403, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1103) [M::mem_pestat] mean and std.dev: (384.45, 205.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1400) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695596 reads in 194.063 CPU sec, 6.399 real sec [M::process] read 695012 sequences (100000200 bp) in 2.054 CPU sec, 2.082 real sec... [M::mem_process_seqs] Processed 695146 reads in 129.037 CPU sec, 4.090 real sec sorting block 54, #reads = 1077011 mem = 536878096 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 283543, 23, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (444, 582, 1196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2700) [M::mem_pestat] mean and std.dev: (845.39, 606.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (279.21, 141.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (398, 1114, 1818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4658) [M::mem_pestat] mean and std.dev: (1032.55, 1104.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6078) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 792, 1157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3077) [M::mem_pestat] mean and std.dev: (733.00, 616.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4037) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693944 sequences (100000027 bp) in 3.142 CPU sec, 3.408 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 282759, 28, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (238, 345, 1000) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2524) [M::mem_pestat] mean and std.dev: (653.24, 725.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (278.62, 140.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (572, 1365, 3237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8567) [M::mem_pestat] mean and std.dev: (1633.63, 1474.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11232) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 303, 1865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5263) [M::mem_pestat] mean and std.dev: (763.36, 867.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6962) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695136 reads in 195.018 CPU sec, 6.252 real sec [M::process] read 694502 sequences (100000014 bp) in 2.172 CPU sec, 2.205 real sec... [M::mem_process_seqs] Processed 695012 reads in 129.860 CPU sec, 4.275 real sec sorting block 55, #reads = 1075417 mem = 536878940 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282325, 18, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (315, 501, 883) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2019) [M::mem_pestat] mean and std.dev: (457.31, 258.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 254, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (280.45, 140.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (228, 528, 1127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2925) [M::mem_pestat] mean and std.dev: (547.20, 662.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3824) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 413, 1888) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5246) [M::mem_pestat] mean and std.dev: (771.69, 989.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6925) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695510 sequences (100000030 bp) in 2.936 CPU sec, 3.142 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282816, 20, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 276, 709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1855) [M::mem_pestat] mean and std.dev: (381.50, 374.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2428) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 252, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (279.00, 140.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (289, 598, 1028) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2506) [M::mem_pestat] mean and std.dev: (720.35, 629.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3245) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (258, 454, 1148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2928) [M::mem_pestat] mean and std.dev: (569.80, 583.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3818) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695660 sequences (100000110 bp) in 2.014 CPU sec, 2.020 real sec... [M::mem_process_seqs] Processed 693944 reads in 198.944 CPU sec, 6.509 real sec [M::mem_process_seqs] Processed 694502 reads in 131.285 CPU sec, 4.176 real sec sorting block 56, #reads = 1075727 mem = 536879230 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 283118, 22, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (256, 373, 1003) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2497) [M::mem_pestat] mean and std.dev: (612.14, 561.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3244) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 247, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (272.78, 136.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (235, 642, 1391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3703) [M::mem_pestat] mean and std.dev: (836.38, 727.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4859) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (284, 367, 619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1289) [M::mem_pestat] mean and std.dev: (449.69, 297.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1640) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 57, #reads = 1075145 mem = 536878645 peak = 897064960 [M::process] read 695356 sequences (100000091 bp) in 3.009 CPU sec, 3.261 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282880, 20, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 271, 596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1482) [M::mem_pestat] mean and std.dev: (387.45, 344.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1925) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 246, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (272.94, 136.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (121, 409, 1155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3223) [M::mem_pestat] mean and std.dev: (686.20, 756.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4257) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 416, 1219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3253) [M::mem_pestat] mean and std.dev: (716.41, 640.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4270) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695324 sequences (100000138 bp) in 2.020 CPU sec, 2.025 real sec... [M::mem_process_seqs] Processed 695510 reads in 197.852 CPU sec, 6.380 real sec [M::mem_process_seqs] Processed 695660 reads in 130.882 CPU sec, 4.220 real sec sorting block 58, #reads = 1075527 mem = 536881405 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282637, 29, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 496, 934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2532) [M::mem_pestat] mean and std.dev: (578.51, 571.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3331) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 247, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (272.63, 136.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (158, 734, 1099) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2981) [M::mem_pestat] mean and std.dev: (691.31, 556.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3922) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 842, 1410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3780) [M::mem_pestat] mean and std.dev: (830.10, 671.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4965) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695474 sequences (100000084 bp) in 2.912 CPU sec, 3.125 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 283402, 23, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 280, 968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2558) [M::mem_pestat] mean and std.dev: (554.31, 567.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3353) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 247, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (273.13, 136.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (160, 423, 1733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4879) [M::mem_pestat] mean and std.dev: (910.22, 944.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6452) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 367, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (312.00, 156.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695804 sequences (100000161 bp) in 2.079 CPU sec, 2.085 real sec... [M::mem_process_seqs] Processed 695356 reads in 199.843 CPU sec, 6.442 real sec sorting block 59, #reads = 1075525 mem = 536877869 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 282893, 33, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 322, 832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2216) [M::mem_pestat] mean and std.dev: (461.86, 433.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2908) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 247, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (271.42, 134.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (214, 683, 1759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4849) [M::mem_pestat] mean and std.dev: (947.50, 977.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6394) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 317, 561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1259) [M::mem_pestat] mean and std.dev: (342.16, 255.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1608) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695274 sequences (100000235 bp) in 2.319 CPU sec, 2.323 real sec... [M::mem_process_seqs] Processed 695474 reads in 114.261 CPU sec, 3.713 real sec [M::mem_process_seqs] Processed 695324 reads in 294.813 CPU sec, 9.490 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 283662, 31, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 318, 972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2608) [M::mem_pestat] mean and std.dev: (486.09, 521.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (272.30, 136.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (217, 620, 1747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4807) [M::mem_pestat] mean and std.dev: (801.07, 831.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6337) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 348, 1584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4386) [M::mem_pestat] mean and std.dev: (697.22, 886.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5787) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 60, #reads = 1074952 mem = 536878399 peak = 897064960 [M::process] read 695694 sequences (100000020 bp) in 3.090 CPU sec, 3.449 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 283142, 25, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 319, 551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1297) [M::mem_pestat] mean and std.dev: (370.41, 256.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (274.78, 138.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (338, 436, 1235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3029) [M::mem_pestat] mean and std.dev: (666.46, 506.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3926) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 376, 1163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3055) [M::mem_pestat] mean and std.dev: (702.96, 635.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4001) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695804 reads in 216.590 CPU sec, 6.915 real sec [M::mem_process_seqs] Processed 695274 reads in 132.750 CPU sec, 4.218 real sec [M::process] read 695734 sequences (100000260 bp) in 2.493 CPU sec, 2.638 real sec... sorting block 61, #reads = 1075907 mem = 536884232 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 283632, 27, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 312, 882) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2226) [M::mem_pestat] mean and std.dev: (579.15, 552.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2898) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 247, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (273.05, 136.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (248, 684, 1643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4433) [M::mem_pestat] mean and std.dev: (1032.85, 1211.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5879) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 322, 1158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3084) [M::mem_pestat] mean and std.dev: (595.63, 499.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4047) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694214 sequences (100000245 bp) in 3.695 CPU sec, 4.108 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 283557, 24, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 394, 651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1557) [M::mem_pestat] mean and std.dev: (457.73, 365.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2010) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (274.88, 138.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (229, 970, 2458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6916) [M::mem_pestat] mean and std.dev: (1195.42, 1204.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9145) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 275, 788) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1914) [M::mem_pestat] mean and std.dev: (379.73, 291.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2477) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695694 reads in 195.182 CPU sec, 6.260 real sec [M::mem_process_seqs] Processed 695734 reads in 130.457 CPU sec, 4.154 real sec sorting block 62, #reads = 1075950 mem = 536877107 peak = 897064960 [M::process] read 695874 sequences (100000132 bp) in 2.813 CPU sec, 3.066 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 282625, 27, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (293, 592, 1350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3464) [M::mem_pestat] mean and std.dev: (616.05, 577.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 254, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (281.11, 142.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (82, 185, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1495) [M::mem_pestat] mean and std.dev: (294.42, 290.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1966) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (278, 406, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013) [M::mem_pestat] mean and std.dev: (404.60, 169.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1258) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703058 sequences (100000019 bp) in 2.956 CPU sec, 3.219 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 283054, 13, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 236, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958) [M::mem_pestat] mean and std.dev: (281.63, 184.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1223) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (275.28, 139.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (66, 574, 2021) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5931) [M::mem_pestat] mean and std.dev: (915.38, 829.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7886) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 332, 871) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2323) [M::mem_pestat] mean and std.dev: (565.44, 590.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3049) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694214 reads in 201.351 CPU sec, 6.476 real sec sorting block 63, #reads = 1076861 mem = 536878672 peak = 897064960 [M::mem_process_seqs] Processed 695874 reads in 138.274 CPU sec, 4.383 real sec [M::process] read 703494 sequences (100000200 bp) in 2.956 CPU sec, 3.160 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 286100, 22, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 386, 718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1784) [M::mem_pestat] mean and std.dev: (447.21, 329.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2317) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 225, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (254.52, 130.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (109, 518, 801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2185) [M::mem_pestat] mean and std.dev: (514.50, 424.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2877) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 229, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (279.37, 165.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1196) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 64, #reads = 1075109 mem = 536879520 peak = 897064960 [M::process] read 701892 sequences (100000110 bp) in 3.354 CPU sec, 3.807 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 286136, 19, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 318, 1096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2930) [M::mem_pestat] mean and std.dev: (609.25, 623.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3847) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 223, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (252.63, 129.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (438, 540, 2505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6639) [M::mem_pestat] mean and std.dev: (1152.79, 1155.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8706) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 302, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (276.81, 152.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 703058 reads in 213.872 CPU sec, 6.859 real sec [M::mem_process_seqs] Processed 703494 reads in 144.652 CPU sec, 4.606 real sec [M::process] read 701206 sequences (100000166 bp) in 2.548 CPU sec, 2.725 real sec... sorting block 65, #reads = 1076065 mem = 536880320 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 286115, 28, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 282, 1387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3799) [M::mem_pestat] mean and std.dev: (582.63, 684.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5005) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 228, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (257.71, 132.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (123, 407, 2220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6414) [M::mem_pestat] mean and std.dev: (1142.19, 1476.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8511) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 222, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052) [M::mem_pestat] mean and std.dev: (312.48, 207.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1337) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699716 sequences (100000040 bp) in 3.171 CPU sec, 3.474 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 286069, 21, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (413, 653, 1426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3452) [M::mem_pestat] mean and std.dev: (732.27, 499.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 231, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (261.36, 135.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 912) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (549, 1339, 2018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4956) [M::mem_pestat] mean and std.dev: (1260.76, 816.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6425) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (292, 390, 1987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5377) [M::mem_pestat] mean and std.dev: (1264.38, 1481.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7191) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701892 reads in 212.061 CPU sec, 6.827 real sec [M::mem_process_seqs] Processed 701206 reads in 141.348 CPU sec, 4.494 real sec [M::process] read 699216 sequences (100000277 bp) in 2.234 CPU sec, 2.319 real sec... sorting block 66, #reads = 1076697 mem = 536880199 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 285204, 30, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 482, 1759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4751) [M::mem_pestat] mean and std.dev: (942.00, 1057.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6247) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 236, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (266.13, 137.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (66, 232, 609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1695) [M::mem_pestat] mean and std.dev: (287.40, 305.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2238) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 395, 1039) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2635) [M::mem_pestat] mean and std.dev: (540.42, 451.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3433) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697736 sequences (100000140 bp) in 3.573 CPU sec, 3.874 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 284828, 11, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 587, 751) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1789) [M::mem_pestat] mean and std.dev: (503.26, 265.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2308) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 238, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (269.18, 139.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1447, 1664, 2079) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (183, 3343) [M::mem_pestat] mean and std.dev: (1622.20, 634.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4161) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 492, 969) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2513) [M::mem_pestat] mean and std.dev: (481.22, 392.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3285) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699716 reads in 208.031 CPU sec, 6.639 real sec [M::process] read 698016 sequences (100000104 bp) in 2.268 CPU sec, 2.298 real sec... sorting block 67, #reads = 1075901 mem = 536877529 peak = 897064960 [M::mem_process_seqs] Processed 699216 reads in 138.079 CPU sec, 4.548 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 283899, 19, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 420, 751) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1771) [M::mem_pestat] mean and std.dev: (411.00, 241.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2281) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 242, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (273.39, 142.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (862, 1420, 2282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5122) [M::mem_pestat] mean and std.dev: (1545.50, 935.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6542) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 359, 955) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2421) [M::mem_pestat] mean and std.dev: (603.96, 574.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3154) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 68, #reads = 1076491 mem = 536876835 peak = 897064960 [M::process] read 698154 sequences (100000010 bp) in 3.013 CPU sec, 3.391 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 284520, 20, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 419, 835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2187) [M::mem_pestat] mean and std.dev: (503.72, 462.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2863) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 242, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (273.97, 142.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (722, 1237, 2162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5042) [M::mem_pestat] mean and std.dev: (1371.65, 1043.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6482) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (322, 515, 1270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3166) [M::mem_pestat] mean and std.dev: (748.36, 620.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4114) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697904 sequences (100000280 bp) in 2.150 CPU sec, 2.147 real sec... [M::mem_process_seqs] Processed 697736 reads in 207.360 CPU sec, 6.841 real sec [M::mem_process_seqs] Processed 698016 reads in 140.878 CPU sec, 4.485 real sec sorting block 69, #reads = 1075503 mem = 536878136 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 284977, 12, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 472, 1898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5262) [M::mem_pestat] mean and std.dev: (1158.37, 1269.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6944) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 242, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (273.24, 141.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (149, 801, 1326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3680) [M::mem_pestat] mean and std.dev: (665.82, 745.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4857) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (277, 433, 1225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3121) [M::mem_pestat] mean and std.dev: (713.07, 597.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4069) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697540 sequences (100000212 bp) in 3.160 CPU sec, 3.368 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 284326, 28, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 370, 669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1635) [M::mem_pestat] mean and std.dev: (379.72, 309.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2118) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 242, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (271.87, 139.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (266, 514, 974) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2390) [M::mem_pestat] mean and std.dev: (486.71, 383.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3098) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 346, 828) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2118) [M::mem_pestat] mean and std.dev: (472.06, 420.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2763) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697626 sequences (100000014 bp) in 2.165 CPU sec, 2.183 real sec... [M::mem_process_seqs] Processed 698154 reads in 219.733 CPU sec, 7.048 real sec [M::mem_process_seqs] Processed 697904 reads in 149.531 CPU sec, 4.811 real sec sorting block 70, #reads = 1075112 mem = 536879878 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 284462, 24, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 291, 555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1315) [M::mem_pestat] mean and std.dev: (308.68, 237.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 242, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (272.88, 140.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (116, 538, 1388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3932) [M::mem_pestat] mean and std.dev: (1018.12, 1201.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5824) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 266, 617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1425) [M::mem_pestat] mean and std.dev: (299.80, 231.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1829) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698120 sequences (100000173 bp) in 3.436 CPU sec, 3.738 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 284576, 19, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 483, 1076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2866) [M::mem_pestat] mean and std.dev: (599.57, 573.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 243, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (273.31, 140.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (272, 831, 2639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7373) [M::mem_pestat] mean and std.dev: (923.56, 965.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9740) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 265, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1160) [M::mem_pestat] mean and std.dev: (311.65, 208.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1485) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696706 sequences (100000073 bp) in 2.180 CPU sec, 2.186 real sec... [M::mem_process_seqs] Processed 697540 reads in 212.656 CPU sec, 6.838 real sec [M::mem_process_seqs] Processed 697626 reads in 138.895 CPU sec, 4.403 real sec sorting block 71, #reads = 1071887 mem = 536879019 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 284456, 24, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 231, 869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2279) [M::mem_pestat] mean and std.dev: (377.11, 404.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2984) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 239, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (269.57, 138.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (171, 448, 787) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2019) [M::mem_pestat] mean and std.dev: (411.38, 358.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2635) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 281, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1117) [M::mem_pestat] mean and std.dev: (327.16, 190.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1409) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 72, #reads = 1074657 mem = 536877593 peak = 897064960 [M::process] read 696814 sequences (100000249 bp) in 2.767 CPU sec, 3.247 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 283375, 29, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (379, 507, 1159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2719) [M::mem_pestat] mean and std.dev: (635.17, 473.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (273.96, 140.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (156, 378, 775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2013) [M::mem_pestat] mean and std.dev: (499.18, 426.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2632) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 328, 757) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1829) [M::mem_pestat] mean and std.dev: (487.67, 429.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2365) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696096 sequences (100000017 bp) in 2.013 CPU sec, 2.020 real sec... [M::mem_process_seqs] Processed 698120 reads in 212.205 CPU sec, 6.857 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 283229, 25, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 367, 945) [M::process] read 696254 sequences (100000266 bp) in 2.313 CPU sec, 2.315 real sec... [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2499) [M::mem_pestat] mean and std.dev: (585.55, 570.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3276) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 244, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (273.79, 140.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (626, 1437, 3310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8678) [M::mem_pestat] mean and std.dev: (2225.32, 2252.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11362) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 582, 1172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3044) [M::mem_pestat] mean and std.dev: (725.12, 723.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3980) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696814 reads in 123.809 CPU sec, 4.022 real sec sorting block 73, #reads = 1074118 mem = 536878166 peak = 897064960 [M::mem_process_seqs] Processed 696706 reads in 306.910 CPU sec, 9.891 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 283251, 28, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 285, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (310.86, 198.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1184) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (276.46, 141.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (538, 1476, 2065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5119) [M::mem_pestat] mean and std.dev: (1244.08, 902.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6646) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 560, 1075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2781) [M::mem_pestat] mean and std.dev: (734.67, 633.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3634) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 74, #reads = 1071941 mem = 536881546 peak = 897064960 [M::process] read 696166 sequences (100000081 bp) in 2.872 CPU sec, 3.250 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282826, 28, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 565, 3259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9247) [M::mem_pestat] mean and std.dev: (1444.34, 1416.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12241) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 244, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (274.25, 140.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (208, 421, 1311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3517) [M::mem_pestat] mean and std.dev: (809.54, 977.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4720) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 352, 551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1277) [M::mem_pestat] mean and std.dev: (375.57, 278.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1640) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695400 sequences (100000262 bp) in 2.165 CPU sec, 2.168 real sec... [M::mem_process_seqs] Processed 696096 reads in 242.187 CPU sec, 7.727 real sec [M::mem_process_seqs] Processed 696254 reads in 148.636 CPU sec, 4.922 real sec sorting block 75, #reads = 1072197 mem = 536881549 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 283458, 23, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 206, 673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1709) [M::mem_pestat] mean and std.dev: (296.14, 218.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2227) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 245, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (274.47, 140.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (399, 789, 1835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4707) [M::mem_pestat] mean and std.dev: (1053.05, 876.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6143) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 362, 1421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3887) [M::mem_pestat] mean and std.dev: (789.32, 929.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5120) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695816 sequences (100000228 bp) in 3.009 CPU sec, 3.410 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 282847, 26, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 420, 985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2579) [M::mem_pestat] mean and std.dev: (602.34, 671.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3376) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (278.30, 142.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (270, 752, 2194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6042) [M::mem_pestat] mean and std.dev: (1492.08, 1706.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8320) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 273, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962) [M::mem_pestat] mean and std.dev: (321.91, 171.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1225) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695180 sequences (100000135 bp) in 2.272 CPU sec, 2.277 real sec... [M::mem_process_seqs] Processed 696166 reads in 221.104 CPU sec, 7.268 real sec [M::mem_process_seqs] Processed 695400 reads in 146.190 CPU sec, 4.699 real sec sorting block 76, #reads = 1071089 mem = 536876352 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 283334, 26, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (269, 400, 754) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1724) [M::mem_pestat] mean and std.dev: (510.14, 407.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2209) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 247, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (277.03, 142.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (421, 1206, 1758) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4432) [M::mem_pestat] mean and std.dev: (1214.31, 912.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5769) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 302, 583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1287) [M::mem_pestat] mean and std.dev: (399.12, 274.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1639) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695460 sequences (100000112 bp) in 2.932 CPU sec, 3.195 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 283040, 13, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 611, 1873) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5149) [M::mem_pestat] mean and std.dev: (1442.65, 1631.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7970) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (279.49, 143.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (57, 376, 1211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3519) [M::mem_pestat] mean and std.dev: (611.42, 728.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4673) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 292, 992) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2578) [M::mem_pestat] mean and std.dev: (515.02, 468.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3371) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 706788 sequences (100000162 bp) in 2.152 CPU sec, 2.153 real sec... [M::mem_process_seqs] Processed 695816 reads in 217.038 CPU sec, 6.991 real sec [M::mem_process_seqs] Processed 695180 reads in 142.458 CPU sec, 4.605 real sec sorting block 77, #reads = 1070466 mem = 536877624 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282731, 19, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 353, 603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1377) [M::mem_pestat] mean and std.dev: (374.24, 241.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1764) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (278.25, 143.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (143, 818, 4677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13745) [M::mem_pestat] mean and std.dev: (2256.37, 3018.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18279) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 435, 806) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1994) [M::mem_pestat] mean and std.dev: (498.10, 421.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2588) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 705280 sequences (100000216 bp) in 3.099 CPU sec, 3.523 real sec... sorting block 78, #reads = 1071449 mem = 536883480 peak = 897064960 [M::process] read 703620 sequences (100000119 bp) in 2.175 CPU sec, 2.183 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 286885, 22, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (280, 711, 902) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2146) [M::mem_pestat] mean and std.dev: (631.79, 404.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 214, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (242.24, 122.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (159, 474, 970) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2592) [M::mem_pestat] mean and std.dev: (514.84, 511.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3403) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (248, 495, 1213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3143) [M::mem_pestat] mean and std.dev: (623.33, 481.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4108) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695460 reads in 236.925 CPU sec, 7.672 real sec [M::mem_process_seqs] Processed 706788 reads in 163.289 CPU sec, 5.202 real sec sorting block 79, #reads = 1069171 mem = 536883337 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 286825, 18, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 305, 595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1507) [M::mem_pestat] mean and std.dev: (369.39, 294.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1963) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 219, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (247.48, 125.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (404, 703, 1396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3380) [M::mem_pestat] mean and std.dev: (876.94, 649.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4372) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 1014, 1305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3481) [M::mem_pestat] mean and std.dev: (812.00, 622.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4569) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703228 sequences (100000295 bp) in 2.663 CPU sec, 2.964 real sec... [M::process] read 703420 sequences (100000220 bp) in 2.193 CPU sec, 2.202 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 286480, 30, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 384, 2203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6153) [M::mem_pestat] mean and std.dev: (833.00, 911.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8128) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 224, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (252.86, 128.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (257, 896, 1392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3662) [M::mem_pestat] mean and std.dev: (837.21, 693.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4797) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 340, 849) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2107) [M::mem_pestat] mean and std.dev: (545.63, 539.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2736) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 705280 reads in 234.137 CPU sec, 7.517 real sec [M::mem_process_seqs] Processed 703620 reads in 157.572 CPU sec, 5.285 real sec sorting block 80, #reads = 1072382 mem = 536877529 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 286022, 13, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 384, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (280.97, 121.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1187) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 226, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (253.72, 128.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (173, 1193, 2666) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7652) [M::mem_pestat] mean and std.dev: (1265.62, 1230.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10145) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (245, 535, 1003) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2519) [M::mem_pestat] mean and std.dev: (688.97, 562.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3277) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701754 sequences (100000182 bp) in 3.501 CPU sec, 3.742 real sec... [M::process] read 700784 sequences (100000064 bp) in 2.143 CPU sec, 2.148 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 286940, 30, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 307, 1024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2650) [M::mem_pestat] mean and std.dev: (493.37, 384.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 226, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (253.66, 128.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (388, 775, 2434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6526) [M::mem_pestat] mean and std.dev: (850.29, 742.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8572) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (363, 552, 1292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3150) [M::mem_pestat] mean and std.dev: (618.67, 412.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4079) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 703228 reads in 240.914 CPU sec, 7.958 real sec [M::mem_process_seqs] Processed 703420 reads in 162.442 CPU sec, 5.196 real sec sorting block 81, #reads = 1071795 mem = 536887033 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 285700, 20, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 540, 775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1823) [M::mem_pestat] mean and std.dev: (457.91, 269.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2347) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 229, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (257.90, 130.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (195, 468, 1056) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2778) [M::mem_pestat] mean and std.dev: (410.29, 366.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3639) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (244, 406, 818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1966) [M::mem_pestat] mean and std.dev: (547.03, 412.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2540) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700508 sequences (100000267 bp) in 3.263 CPU sec, 3.505 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 285856, 27, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 449, 901) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2201) [M::mem_pestat] mean and std.dev: (629.74, 603.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3045) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 233, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (261.72, 133.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (370, 544, 1177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2791) [M::mem_pestat] mean and std.dev: (524.82, 351.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3598) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (268, 362, 631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1357) [M::mem_pestat] mean and std.dev: (443.78, 251.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1720) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699664 sequences (100000283 bp) in 2.204 CPU sec, 2.207 real sec... [M::mem_process_seqs] Processed 701754 reads in 225.102 CPU sec, 7.183 real sec sorting block 82, #reads = 1069557 mem = 536877817 peak = 897064960 [M::mem_process_seqs] Processed 700784 reads in 149.454 CPU sec, 4.758 real sec sorting block 83, #reads = 1071153 mem = 536878144 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 285181, 17, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 430, 1263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3519) [M::mem_pestat] mean and std.dev: (762.75, 931.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 233, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (262.61, 134.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (374, 677, 1758) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4526) [M::mem_pestat] mean and std.dev: (921.06, 761.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5910) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (253, 611, 748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1738) [M::mem_pestat] mean and std.dev: (492.30, 356.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2233) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699164 sequences (100000239 bp) in 3.034 CPU sec, 3.343 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 284769, 15, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 393, 1786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4944) [M::mem_pestat] mean and std.dev: (1089.58, 1258.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 236, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (264.50, 134.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (357, 481, 1460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3666) [M::mem_pestat] mean and std.dev: (846.20, 746.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4769) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 263, 965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2533) [M::mem_pestat] mean and std.dev: (550.05, 555.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3317) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699760 sequences (100000168 bp) in 2.163 CPU sec, 2.270 real sec... [M::mem_process_seqs] Processed 700508 reads in 229.700 CPU sec, 7.636 real sec [M::mem_process_seqs] Processed 699664 reads in 147.846 CPU sec, 4.783 real sec sorting block 84, #reads = 1071410 mem = 536883153 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 284787, 17, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 312, 806) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2068) [M::mem_pestat] mean and std.dev: (418.02, 308.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 237, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (266.25, 135.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (386, 621, 965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2123) [M::mem_pestat] mean and std.dev: (612.65, 405.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2702) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (289, 468, 1055) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2587) [M::mem_pestat] mean and std.dev: (577.66, 492.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3353) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698618 sequences (100000167 bp) in 2.973 CPU sec, 3.387 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 284959, 18, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 272, 798) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2066) [M::mem_pestat] mean and std.dev: (474.11, 482.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 234, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (263.35, 134.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (248, 559, 1016) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2552) [M::mem_pestat] mean and std.dev: (587.18, 449.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3320) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 390, 1225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3259) [M::mem_pestat] mean and std.dev: (644.50, 593.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4276) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697446 sequences (100000293 bp) in 2.103 CPU sec, 2.105 real sec... [M::mem_process_seqs] Processed 699164 reads in 225.672 CPU sec, 7.337 real sec [M::mem_process_seqs] Processed 699760 reads in 150.453 CPU sec, 4.841 real sec sorting block 85, #reads = 1071633 mem = 536884742 peak = 897064960 [M::process] read 699172 sequences (100000025 bp) in 3.189 CPU sec, 3.546 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 284126, 37, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 453, 666) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1558) [M::mem_pestat] mean and std.dev: (458.54, 280.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2004) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 242, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (272.13, 139.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (378, 796, 1434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3546) [M::mem_pestat] mean and std.dev: (790.09, 686.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4602) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (382, 486, 1156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2704) [M::mem_pestat] mean and std.dev: (763.44, 632.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3478) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696864 sequences (100000045 bp) in 2.435 CPU sec, 2.445 real sec... [M::mem_process_seqs] Processed 697446 reads in 123.860 CPU sec, 3.923 real sec sorting block 86, #reads = 1070957 mem = 536879551 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 284838, 39, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 293, 835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2107) [M::mem_pestat] mean and std.dev: (482.43, 406.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 238, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (267.36, 136.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (399, 658, 1013) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2241) [M::mem_pestat] mean and std.dev: (679.36, 487.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2855) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 303, 637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1551) [M::mem_pestat] mean and std.dev: (376.47, 321.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2008) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 284152, 15, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (264, 353, 658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1446) [M::mem_pestat] mean and std.dev: (481.97, 339.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1840) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 235, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (265.53, 136.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (100, 832, 1988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5764) [M::mem_pestat] mean and std.dev: (1300.40, 1551.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7652) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (271, 341, 736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1666) [M::mem_pestat] mean and std.dev: (404.23, 277.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2131) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698618 reads in 333.064 CPU sec, 10.674 real sec [M::process] read 696792 sequences (100000037 bp) in 2.445 CPU sec, 2.464 real sec... [M::mem_process_seqs] Processed 699172 reads in 169.359 CPU sec, 5.648 real sec sorting block 87, #reads = 1072683 mem = 536879945 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 284076, 17, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 322, 1053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2689) [M::mem_pestat] mean and std.dev: (635.46, 579.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (274.72, 142.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (173, 473, 1563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4343) [M::mem_pestat] mean and std.dev: (524.21, 495.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5733) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 541, 1255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3347) [M::mem_pestat] mean and std.dev: (671.60, 635.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4393) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696848 sequences (100000283 bp) in 2.964 CPU sec, 3.388 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 283868, 28, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 442, 927) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2373) [M::mem_pestat] mean and std.dev: (455.03, 347.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3096) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (275.75, 143.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (404, 598, 1356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3260) [M::mem_pestat] mean and std.dev: (758.27, 663.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4212) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 266, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975) [M::mem_pestat] mean and std.dev: (301.72, 114.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1223) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695636 sequences (100000079 bp) in 2.103 CPU sec, 2.219 real sec... [M::mem_process_seqs] Processed 696864 reads in 215.089 CPU sec, 7.152 real sec sorting block 88, #reads = 1071691 mem = 536882160 peak = 897064960 [M::mem_process_seqs] Processed 696792 reads in 142.796 CPU sec, 4.565 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 283453, 27, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 403, 756) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1920) [M::mem_pestat] mean and std.dev: (475.59, 380.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 244, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (277.37, 145.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (409, 580, 1492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3658) [M::mem_pestat] mean and std.dev: (807.52, 832.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4741) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 329, 556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1232) [M::mem_pestat] mean and std.dev: (343.35, 230.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1570) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 89, #reads = 1072256 mem = 536876811 peak = 897064960 [M::process] read 695586 sequences (100000126 bp) in 2.931 CPU sec, 3.244 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 283177, 43, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 432, 1678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4570) [M::mem_pestat] mean and std.dev: (758.62, 890.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6016) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (279.38, 144.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (388, 802, 1677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4255) [M::mem_pestat] mean and std.dev: (1096.38, 940.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5544) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 349, 605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1335) [M::mem_pestat] mean and std.dev: (409.59, 285.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1700) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695686 sequences (100000143 bp) in 2.195 CPU sec, 2.198 real sec... [M::mem_process_seqs] Processed 696848 reads in 213.608 CPU sec, 6.893 real sec [M::mem_process_seqs] Processed 695636 reads in 142.593 CPU sec, 4.552 real sec sorting block 90, #reads = 1072071 mem = 536877954 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282454, 27, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 457, 847) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2091) [M::mem_pestat] mean and std.dev: (438.12, 299.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (280.09, 146.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 989) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (126, 439, 869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2355) [M::mem_pestat] mean and std.dev: (403.78, 330.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3098) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 198, 687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1737) [M::mem_pestat] mean and std.dev: (376.35, 396.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2262) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702176 sequences (100000083 bp) in 2.781 CPU sec, 3.177 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 283639, 27, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (238, 509, 1622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4390) [M::mem_pestat] mean and std.dev: (888.62, 906.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5774) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (279.96, 145.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 993) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (294, 564, 1349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3459) [M::mem_pestat] mean and std.dev: (851.44, 869.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4514) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 458, 770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1898) [M::mem_pestat] mean and std.dev: (472.82, 401.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2462) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702480 sequences (100000195 bp) in 2.044 CPU sec, 2.053 real sec... [M::mem_process_seqs] Processed 695586 reads in 228.185 CPU sec, 7.433 real sec [M::mem_process_seqs] Processed 695686 reads in 155.675 CPU sec, 5.066 real sec sorting block 91, #reads = 1068533 mem = 536879551 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 285142, 30, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 225, 793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2075) [M::mem_pestat] mean and std.dev: (387.39, 345.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 228, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (257.81, 132.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (264, 621, 1113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2811) [M::mem_pestat] mean and std.dev: (571.21, 504.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3660) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 271, 610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1362) [M::mem_pestat] mean and std.dev: (318.50, 183.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1738) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700784 sequences (100000132 bp) in 3.328 CPU sec, 3.729 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 286214, 27, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (250, 734, 2476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6928) [M::mem_pestat] mean and std.dev: (813.06, 839.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9154) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 228, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (256.79, 131.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (187, 546, 770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1936) [M::mem_pestat] mean and std.dev: (387.00, 282.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2519) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (304, 357, 910) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2122) [M::mem_pestat] mean and std.dev: (451.64, 412.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2728) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699894 sequences (100000069 bp) in 2.218 CPU sec, 2.238 real sec... [M::mem_process_seqs] Processed 702176 reads in 239.372 CPU sec, 7.762 real sec sorting block 92, #reads = 1064522 mem = 536877866 peak = 897064960 [M::mem_process_seqs] Processed 702480 reads in 154.423 CPU sec, 4.934 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 285480, 11, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 361, 967) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2503) [M::mem_pestat] mean and std.dev: (540.97, 497.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3271) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 232, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (261.21, 133.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 908) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (253, 710, 1564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4186) [M::mem_pestat] mean and std.dev: (1045.73, 1075.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5497) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 300, 969) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2529) [M::mem_pestat] mean and std.dev: (524.04, 455.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3309) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 93, #reads = 1073131 mem = 536880831 peak = 897064960 [M::process] read 698998 sequences (100000170 bp) in 2.910 CPU sec, 3.218 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 284915, 32, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 430, 934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2300) [M::mem_pestat] mean and std.dev: (558.48, 459.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2983) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 234, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (263.14, 134.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (272, 1079, 1333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3455) [M::mem_pestat] mean and std.dev: (860.84, 570.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4516) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (308, 601, 803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1793) [M::mem_pestat] mean and std.dev: (599.50, 419.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2288) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698814 sequences (100000272 bp) in 2.038 CPU sec, 2.089 real sec... [M::mem_process_seqs] Processed 700784 reads in 209.144 CPU sec, 6.706 real sec [M::mem_process_seqs] Processed 699894 reads in 137.842 CPU sec, 4.629 real sec sorting block 94, #reads = 1074678 mem = 536878584 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 284639, 19, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 400, 1423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3859) [M::mem_pestat] mean and std.dev: (646.58, 610.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5077) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 238, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (266.77, 136.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (444, 1171, 2489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6579) [M::mem_pestat] mean and std.dev: (1536.06, 1485.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8624) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (246, 358, 1064) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2700) [M::mem_pestat] mean and std.dev: (650.42, 604.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3518) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698374 sequences (100000172 bp) in 3.344 CPU sec, 3.631 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 284877, 41, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 462, 1124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3034) [M::mem_pestat] mean and std.dev: (589.57, 504.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3989) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 238, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (268.59, 138.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (165, 1252, 2217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6321) [M::mem_pestat] mean and std.dev: (1343.29, 1227.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8373) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 248, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1061) [M::mem_pestat] mean and std.dev: (263.53, 121.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1350) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698116 sequences (100000082 bp) in 2.068 CPU sec, 2.111 real sec... [M::mem_process_seqs] Processed 698998 reads in 210.234 CPU sec, 6.995 real sec [M::mem_process_seqs] Processed 698814 reads in 136.771 CPU sec, 4.445 real sec sorting block 95, #reads = 1076027 mem = 536878051 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 284434, 16, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 300, 575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1317) [M::mem_pestat] mean and std.dev: (360.00, 220.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1688) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 240, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (270.76, 140.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (902, 1483, 4552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11852) [M::mem_pestat] mean and std.dev: (2168.88, 1816.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15502) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 599, 1058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2720) [M::mem_pestat] mean and std.dev: (662.65, 578.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3551) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698216 sequences (100000042 bp) in 2.958 CPU sec, 3.479 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 284766, 26, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 271, 2895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8345) [M::mem_pestat] mean and std.dev: (1472.18, 1982.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11070) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 242, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (271.68, 140.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (774, 1747, 5862) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16038) [M::mem_pestat] mean and std.dev: (2558.81, 2269.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21126) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (138, 263, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1296) [M::mem_pestat] mean and std.dev: (346.65, 263.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1682) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698298 sequences (100000129 bp) in 2.030 CPU sec, 2.031 real sec... [M::mem_process_seqs] Processed 698374 reads in 207.518 CPU sec, 6.763 real sec sorting block 96, #reads = 1077209 mem = 536883854 peak = 897064960 [M::mem_process_seqs] Processed 698116 reads in 135.458 CPU sec, 4.354 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 285095, 12, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 221, 627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1569) [M::mem_pestat] mean and std.dev: (325.12, 267.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2040) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 241, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (270.67, 139.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (212, 625, 2190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6146) [M::mem_pestat] mean and std.dev: (925.17, 935.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8124) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (262, 594, 1408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3700) [M::mem_pestat] mean and std.dev: (875.54, 716.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4846) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697228 sequences (100000113 bp) in 2.857 CPU sec, 3.275 real sec... sorting block 97, #reads = 1076528 mem = 536877280 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 284749, 22, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 377, 1097) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2817) [M::mem_pestat] mean and std.dev: (683.60, 651.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 240, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (269.60, 137.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (338, 881, 2040) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5444) [M::mem_pestat] mean and std.dev: (1247.67, 1238.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7146) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 382, 883) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2233) [M::mem_pestat] mean and std.dev: (644.47, 604.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3062) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696630 sequences (100000102 bp) in 2.241 CPU sec, 2.243 real sec... [M::mem_process_seqs] Processed 698216 reads in 216.897 CPU sec, 7.013 real sec [M::mem_process_seqs] Processed 698298 reads in 143.212 CPU sec, 4.623 real sec sorting block 98, #reads = 1075120 mem = 536878912 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 284181, 19, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (229, 294, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (283.95, 104.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 244, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (273.04, 140.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (57, 355, 2086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6144) [M::mem_pestat] mean and std.dev: (1181.84, 1314.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8173) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (331, 499, 980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2278) [M::mem_pestat] mean and std.dev: (601.12, 412.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2927) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697060 sequences (100000146 bp) in 2.867 CPU sec, 3.332 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 284268, 14, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 370, 887) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2283) [M::mem_pestat] mean and std.dev: (456.56, 371.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2981) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (275.28, 140.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (81, 635, 1468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4242) [M::mem_pestat] mean and std.dev: (708.00, 845.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5629) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 311, 943) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2365) [M::mem_pestat] mean and std.dev: (512.36, 508.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3076) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696616 sequences (100000142 bp) in 2.174 CPU sec, 2.228 real sec... [M::mem_process_seqs] Processed 697228 reads in 215.994 CPU sec, 7.049 real sec [M::mem_process_seqs] Processed 696630 reads in 140.272 CPU sec, 4.558 real sec sorting block 99, #reads = 1073583 mem = 536880399 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 283796, 16, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 397, 864) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2090) [M::mem_pestat] mean and std.dev: (462.55, 380.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (274.61, 141.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 437, 907) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2631) [M::mem_pestat] mean and std.dev: (523.93, 627.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3493) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 565, 1005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2647) [M::mem_pestat] mean and std.dev: (722.60, 655.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3468) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696296 sequences (100000128 bp) in 2.974 CPU sec, 3.263 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 283386, 28, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 242, 812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2108) [M::mem_pestat] mean and std.dev: (433.86, 427.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2756) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (275.61, 141.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (437, 778, 1299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3023) [M::mem_pestat] mean and std.dev: (755.75, 532.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3885) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 452, 1138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2960) [M::mem_pestat] mean and std.dev: (752.32, 726.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3871) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696650 sequences (100000172 bp) in 2.171 CPU sec, 2.179 real sec... [M::mem_process_seqs] Processed 697060 reads in 214.003 CPU sec, 6.935 real sec [M::mem_process_seqs] Processed 696616 reads in 139.599 CPU sec, 4.515 real sec sorting block 100, #reads = 1074220 mem = 536882571 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 283597, 24, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 316, 607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1347) [M::mem_pestat] mean and std.dev: (358.12, 235.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (277.25, 143.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (86, 437, 1127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3209) [M::mem_pestat] mean and std.dev: (433.64, 444.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4250) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (266, 353, 1025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2543) [M::mem_pestat] mean and std.dev: (591.04, 607.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3302) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695884 sequences (100000195 bp) in 2.690 CPU sec, 2.968 real sec... sorting block 101, #reads = 1073436 mem = 536879373 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 283171, 41, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 387, 1529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4215) [M::mem_pestat] mean and std.dev: (740.36, 852.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (275.53, 142.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (325, 419, 1223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3019) [M::mem_pestat] mean and std.dev: (629.59, 450.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3917) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 397, 601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1371) [M::mem_pestat] mean and std.dev: (472.89, 336.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1820) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696220 sequences (100000010 bp) in 2.235 CPU sec, 2.294 real sec... [M::mem_process_seqs] Processed 696296 reads in 214.078 CPU sec, 6.943 real sec [M::mem_process_seqs] Processed 696650 reads in 143.211 CPU sec, 4.649 real sec sorting block 102, #reads = 1072339 mem = 536878734 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 283018, 23, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (243, 422, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1107) [M::mem_pestat] mean and std.dev: (357.24, 172.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1395) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 247, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (276.78, 142.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (285, 493, 1279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3267) [M::mem_pestat] mean and std.dev: (755.17, 598.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4261) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 385, 1011) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2627) [M::mem_pestat] mean and std.dev: (644.14, 567.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3435) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696180 sequences (100000166 bp) in 3.024 CPU sec, 3.376 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 283392, 30, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 401, 785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1951) [M::mem_pestat] mean and std.dev: (477.49, 392.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (276.52, 142.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (143, 451, 939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2531) [M::mem_pestat] mean and std.dev: (558.86, 476.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3327) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (284, 457, 989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2399) [M::mem_pestat] mean and std.dev: (619.14, 441.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3104) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696370 sequences (100000169 bp) in 2.084 CPU sec, 2.115 real sec... [M::mem_process_seqs] Processed 695884 reads in 218.054 CPU sec, 7.074 real sec [M::mem_process_seqs] Processed 696220 reads in 145.271 CPU sec, 4.644 real sec sorting block 103, #reads = 1072196 mem = 536877157 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 283102, 16, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (313, 653, 2435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6679) [M::mem_pestat] mean and std.dev: (917.75, 889.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8801) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 245, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (275.88, 142.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (341, 1193, 4250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12068) [M::mem_pestat] mean and std.dev: (2420.12, 2761.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15977) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (273, 796, 1606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4272) [M::mem_pestat] mean and std.dev: (950.40, 863.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5605) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696176 sequences (100000009 bp) in 3.431 CPU sec, 3.849 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283228, 16, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 152, 689) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1803) [M::mem_pestat] mean and std.dev: (372.09, 394.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (274.50, 141.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (247, 1352, 2123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5875) [M::mem_pestat] mean and std.dev: (1227.38, 988.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7751) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (246, 388, 753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1767) [M::mem_pestat] mean and std.dev: (496.24, 385.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2274) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696180 reads in 215.129 CPU sec, 6.903 real sec [M::process] read 696096 sequences (100000223 bp) in 2.354 CPU sec, 2.477 real sec... [M::mem_process_seqs] Processed 696370 reads in 155.399 CPU sec, 4.964 real sec sorting block 104, #reads = 1070949 mem = 536883831 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 283267, 16, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 289, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1143) [M::mem_pestat] mean and std.dev: (334.64, 254.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 245, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (275.83, 142.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (90, 760, 2551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7473) [M::mem_pestat] mean and std.dev: (1492.19, 2006.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9934) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (265, 435, 1491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3943) [M::mem_pestat] mean and std.dev: (608.21, 518.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5169) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703840 sequences (100000185 bp) in 3.051 CPU sec, 3.348 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 282835, 13, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 341, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1065) [M::mem_pestat] mean and std.dev: (342.46, 218.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1344) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (276.60, 143.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (353, 1198, 1616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4142) [M::mem_pestat] mean and std.dev: (1019.00, 777.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5405) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 305, 619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1453) [M::mem_pestat] mean and std.dev: (342.44, 213.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1870) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 707058 sequences (100000075 bp) in 2.301 CPU sec, 2.357 real sec... [M::mem_process_seqs] Processed 696176 reads in 225.995 CPU sec, 7.227 real sec sorting block 105, #reads = 1070796 mem = 536881725 peak = 897064960 [M::mem_process_seqs] Processed 696096 reads in 157.624 CPU sec, 5.264 real sec sorting block 106, #reads = 1068203 mem = 536878199 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 284947, 16, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 354, 891) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2309) [M::mem_pestat] mean and std.dev: (450.57, 401.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3018) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 223, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (251.76, 129.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1150, 1212, 8670) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 23710) [M::mem_pestat] mean and std.dev: (3796.62, 3486.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 31230) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (273, 736, 1016) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2502) [M::mem_pestat] mean and std.dev: (734.86, 559.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3245) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704410 sequences (100000297 bp) in 3.105 CPU sec, 3.437 real sec... [M::process] read 702644 sequences (100000244 bp) in 2.089 CPU sec, 2.097 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 287249, 24, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 363, 896) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2436) [M::mem_pestat] mean and std.dev: (496.36, 454.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3206) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 215, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (242.24, 122.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (197, 486, 4351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12659) [M::mem_pestat] mean and std.dev: (1515.83, 1810.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16813) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 260, 607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1513) [M::mem_pestat] mean and std.dev: (368.21, 361.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1966) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 703840 reads in 255.707 CPU sec, 8.462 real sec [M::mem_process_seqs] Processed 707058 reads in 165.762 CPU sec, 5.301 real sec sorting block 107, #reads = 1062581 mem = 536882271 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 286212, 11, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (290, 503, 1092) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2696) [M::mem_pestat] mean and std.dev: (698.48, 597.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 221, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (250.28, 127.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (116, 185, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1163) [M::mem_pestat] mean and std.dev: (239.50, 178.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1512) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 294, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (295.69, 179.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702186 sequences (100000112 bp) in 2.856 CPU sec, 3.084 real sec... [M::process] read 701960 sequences (100000141 bp) in 2.160 CPU sec, 2.169 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 285706, 19, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 248, 561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1357) [M::mem_pestat] mean and std.dev: (335.26, 242.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 227, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (254.54, 128.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (283, 471, 2313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6373) [M::mem_pestat] mean and std.dev: (1036.42, 1032.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8403) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 322, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (308.46, 150.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 704410 reads in 237.197 CPU sec, 7.701 real sec [M::mem_process_seqs] Processed 702644 reads in 153.375 CPU sec, 4.987 real sec sorting block 108, #reads = 1072034 mem = 536880941 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 285287, 24, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 240, 535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1363) [M::mem_pestat] mean and std.dev: (257.50, 188.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 229, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (257.47, 131.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (122, 330, 1179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3293) [M::mem_pestat] mean and std.dev: (464.50, 544.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4350) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 358, 1304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3566) [M::mem_pestat] mean and std.dev: (590.97, 681.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4697) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700730 sequences (100000055 bp) in 3.827 CPU sec, 4.240 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 285576, 21, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (264, 378, 967) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2373) [M::mem_pestat] mean and std.dev: (465.21, 354.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3076) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 228, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (258.44, 132.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (333, 452, 1123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2703) [M::mem_pestat] mean and std.dev: (677.60, 615.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3493) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 428, 728) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1716) [M::mem_pestat] mean and std.dev: (436.07, 239.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2210) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699464 sequences (100000115 bp) in 2.137 CPU sec, 2.139 real sec... [M::mem_process_seqs] Processed 702186 reads in 239.590 CPU sec, 7.710 real sec sorting block 109, #reads = 1069592 mem = 536880349 peak = 897064960 [M::mem_process_seqs] Processed 701960 reads in 162.962 CPU sec, 5.188 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 284517, 22, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 395, 706) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1636) [M::mem_pestat] mean and std.dev: (484.80, 316.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2101) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 232, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (262.43, 134.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (325, 877, 1060) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2530) [M::mem_pestat] mean and std.dev: (783.48, 509.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3265) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 302, 650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1600) [M::mem_pestat] mean and std.dev: (369.08, 289.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2075) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 110, #reads = 1069338 mem = 536878724 peak = 897064960 [M::process] read 699772 sequences (100000257 bp) in 4.754 CPU sec, 5.156 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 284713, 16, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 549, 1403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3791) [M::mem_pestat] mean and std.dev: (835.64, 834.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4985) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 235, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (265.93, 136.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (100, 233, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (172.29, 124.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 484, 715) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1751) [M::mem_pestat] mean and std.dev: (524.09, 405.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2269) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700730 reads in 224.627 CPU sec, 7.182 real sec [M::mem_process_seqs] Processed 699464 reads in 152.051 CPU sec, 4.815 real sec [M::process] read 698690 sequences (100000153 bp) in 2.454 CPU sec, 2.556 real sec... sorting block 111, #reads = 1072085 mem = 536881389 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 284789, 12, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (260, 422, 872) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2096) [M::mem_pestat] mean and std.dev: (439.64, 313.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2708) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 234, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (263.68, 134.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (389, 873, 1984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5174) [M::mem_pestat] mean and std.dev: (830.80, 652.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6769) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 612, 1050) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2722) [M::mem_pestat] mean and std.dev: (741.37, 657.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3558) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696588 sequences (100000145 bp) in 3.468 CPU sec, 3.746 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 283866, 15, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 344, 959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2491) [M::mem_pestat] mean and std.dev: (534.60, 475.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3257) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 237, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (266.21, 135.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (395, 800, 1604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4022) [M::mem_pestat] mean and std.dev: (852.57, 709.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5231) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 245, 876) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2226) [M::mem_pestat] mean and std.dev: (539.91, 500.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2901) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698290 sequences (100000023 bp) in 2.065 CPU sec, 2.071 real sec... [M::mem_process_seqs] Processed 699772 reads in 225.951 CPU sec, 7.206 real sec [M::mem_process_seqs] Processed 698690 reads in 144.890 CPU sec, 4.681 real sec sorting block 112, #reads = 1071237 mem = 536880875 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283526, 34, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 358, 586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1398) [M::mem_pestat] mean and std.dev: (335.36, 196.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1804) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (274.88, 140.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (424, 627, 1157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2623) [M::mem_pestat] mean and std.dev: (723.69, 505.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3356) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 326, 1317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3533) [M::mem_pestat] mean and std.dev: (663.26, 707.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4641) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697638 sequences (100000137 bp) in 3.036 CPU sec, 3.255 real sec... [M::process] read 696698 sequences (100000076 bp) in 2.060 CPU sec, 2.060 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 283996, 21, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 259, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1195) [M::mem_pestat] mean and std.dev: (303.20, 203.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 238, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (267.36, 136.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (305, 642, 2618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7244) [M::mem_pestat] mean and std.dev: (937.56, 1104.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9557) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 516, 2772) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7852) [M::mem_pestat] mean and std.dev: (1647.10, 2152.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10392) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696588 reads in 211.796 CPU sec, 6.831 real sec sorting block 113, #reads = 1069160 mem = 536877770 peak = 897064960 [M::mem_process_seqs] Processed 698290 reads in 140.427 CPU sec, 4.545 real sec sorting block 114, #reads = 1069689 mem = 536880971 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 283376, 31, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 344, 4289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12525) [M::mem_pestat] mean and std.dev: (1557.81, 2136.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 241, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (270.33, 137.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (326, 555, 2656) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7316) [M::mem_pestat] mean and std.dev: (1266.07, 1326.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9646) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 338, 1323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3593) [M::mem_pestat] mean and std.dev: (898.70, 1034.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5037) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696674 sequences (100000062 bp) in 2.830 CPU sec, 2.995 real sec... [M::process] read 696638 sequences (100000227 bp) in 2.091 CPU sec, 2.098 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 283724, 22, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 363, 776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1886) [M::mem_pestat] mean and std.dev: (427.65, 285.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 243, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (271.24, 137.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (335, 1365, 2058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5504) [M::mem_pestat] mean and std.dev: (980.37, 766.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7227) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 283, 601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1405) [M::mem_pestat] mean and std.dev: (356.76, 237.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1807) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697638 reads in 215.089 CPU sec, 6.935 real sec [M::mem_process_seqs] Processed 696698 reads in 140.845 CPU sec, 4.538 real sec sorting block 115, #reads = 1069448 mem = 536881929 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 283676, 21, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 429, 990) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2616) [M::mem_pestat] mean and std.dev: (594.48, 647.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3429) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 243, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (271.73, 138.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (341, 666, 1159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2795) [M::mem_pestat] mean and std.dev: (714.40, 589.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3613) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 421, 1570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4364) [M::mem_pestat] mean and std.dev: (711.05, 670.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5761) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696828 sequences (100000135 bp) in 2.808 CPU sec, 2.961 real sec... [M::process] read 697132 sequences (100000003 bp) in 1.933 CPU sec, 1.935 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 283442, 38, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 439, 1452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3974) [M::mem_pestat] mean and std.dev: (916.53, 1014.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5235) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 243, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (272.18, 138.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (175, 496, 2198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6244) [M::mem_pestat] mean and std.dev: (1322.55, 1520.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8267) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 355, 943) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2413) [M::mem_pestat] mean and std.dev: (458.21, 366.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3148) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696674 reads in 213.808 CPU sec, 6.877 real sec [M::mem_process_seqs] Processed 696638 reads in 139.987 CPU sec, 4.533 real sec sorting block 116, #reads = 1072414 mem = 536879615 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283691, 21, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 321, 607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1441) [M::mem_pestat] mean and std.dev: (354.38, 249.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1858) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 242, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (272.71, 140.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (298, 581, 1679) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4441) [M::mem_pestat] mean and std.dev: (794.15, 709.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5822) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 263, 656) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1634) [M::mem_pestat] mean and std.dev: (371.75, 317.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2123) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696750 sequences (100000037 bp) in 2.862 CPU sec, 3.044 real sec... [M::process] read 695178 sequences (100000163 bp) in 1.858 CPU sec, 1.863 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 283802, 24, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 329, 661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1519) [M::mem_pestat] mean and std.dev: (440.85, 333.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1948) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 241, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (272.21, 140.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (364, 590, 747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1513) [M::mem_pestat] mean and std.dev: (509.36, 325.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1896) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (244, 751, 1935) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5317) [M::mem_pestat] mean and std.dev: (1370.67, 1633.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7905) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696828 reads in 214.557 CPU sec, 6.928 real sec sorting block 117, #reads = 1072336 mem = 536880005 peak = 897064960 [M::mem_process_seqs] Processed 697132 reads in 140.637 CPU sec, 4.572 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 283352, 21, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (276, 390, 1043) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2577) [M::mem_pestat] mean and std.dev: (670.29, 634.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3344) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 243, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (273.42, 140.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (215, 587, 2107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5891) [M::mem_pestat] mean and std.dev: (1122.33, 1160.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7783) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (276, 1114, 1997) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5439) [M::mem_pestat] mean and std.dev: (1215.64, 1120.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7160) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 118, #reads = 1072357 mem = 536877477 peak = 897064960 [M::process] read 696066 sequences (100000058 bp) in 3.078 CPU sec, 3.243 real sec... [M::process] read 213982 sequences (30693419 bp) in 0.872 CPU sec, 0.875 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 282700, 34, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (268, 446, 702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1570) [M::mem_pestat] mean and std.dev: (401.06, 210.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2004) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 250, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784) [M::mem_pestat] mean and std.dev: (280.86, 145.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 990) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (55, 287, 773) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2209) [M::mem_pestat] mean and std.dev: (348.77, 373.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2927) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 506, 1039) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2759) [M::mem_pestat] mean and std.dev: (592.52, 526.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3619) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696750 reads in 217.726 CPU sec, 7.066 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 283505, 31, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 316, 997) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2627) [M::mem_pestat] mean and std.dev: (636.37, 686.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (275.95, 141.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (311, 434, 1443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3707) [M::mem_pestat] mean and std.dev: (972.35, 977.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4883) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 318, 871) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2239) [M::mem_pestat] mean and std.dev: (479.83, 450.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2923) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696066 reads in 120.805 CPU sec, 4.008 real sec sorting block 119, #reads = 1071002 mem = 536883263 peak = 897064960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 87018, 12, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 442, 1547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4151) [M::mem_pestat] mean and std.dev: (720.96, 706.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 242, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (271.14, 138.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (328, 476, 544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976) [M::mem_pestat] mean and std.dev: (354.55, 209.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1192) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (277, 358, 1720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4606) [M::mem_pestat] mean and std.dev: (646.42, 747.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6049) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 213982 reads in 65.183 CPU sec, 2.144 real sec [M::mem_process_seqs] Processed 695178 reads in 298.785 CPU sec, 10.109 real sec sorting block 120, #reads = 1069009 mem = 536877842 peak = 897064960 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 129213598 overall: 69428469760 mem 23006.885 user 960.907 sys 794.194 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_4GR-T1-TDNA-1_A23MHWWLT4_1.L007\tPU:L007\tSM:1136_4GR-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_4GR-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1136_4GR-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 1136_4GR-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 794.237 sec; CPU: 23967.799 sec sorting block 121, #reads = 330368 mem = 165850344 peak = 897064960 uniq header count 1 merging 122 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 384 algo: util-sort license: sentieon:util=1 output file size: 7460105052 output reads: 130295262 bam_mem_sort: 122 calls 106.629 user 2.666 sys 110.788 real bam_write: 122 calls 74.697 user 6.596 sys 117.460 real execute: 1 calls 46.685 user 3.016 sys 847.132 real merge_files: 1 calls 46.654 user 2.995 sys 52.607 real parse_chunk: 1941 calls 92.232 user 26.788 sys 135.965 real read_chunk: 5250 calls 66.701 user 29.308 sys 713.871 real sort_block: 1 calls 47.726 user 27.962 sys 687.429 real write_chunk: 3307 calls 46.169 user 2.637 sys 51.443 real overall: 3085201408 mem 2023.497 user 114.740 sys 847.153 real ls: cannot access '*.cram': No such file or directory