This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1029_BCAN-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1201.142 CPU sec, 41.638 real sec [M::process] read 869700 sequences (100000008 bp) in 1.085 CPU sec, 1.094 real sec... [M::process] read 869744 sequences (100000087 bp) in 1.640 CPU sec, 1.638 real sec... [M::process] read 868858 sequences (100000082 bp) in 1.710 CPU sec, 1.708 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 292186, 51, 90) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 544, 1149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3115) [M::mem_pestat] mean and std.dev: (805.17, 797.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4098) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (123.16, 44.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (259, 734, 1749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4729) [M::mem_pestat] mean and std.dev: (879.54, 867.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6219) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (247, 441, 1212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3142) [M::mem_pestat] mean and std.dev: (620.55, 638.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4107) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 866356 sequences (100000055 bp) in 1.700 CPU sec, 1.700 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 293940, 46, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 406, 1315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3555) [M::mem_pestat] mean and std.dev: (717.74, 670.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (123.28, 44.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (241, 694, 1263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3307) [M::mem_pestat] mean and std.dev: (862.71, 788.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4329) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 558, 1377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3737) [M::mem_pestat] mean and std.dev: (838.16, 830.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4917) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 293352, 51, 73) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (266, 481, 1322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3434) [M::mem_pestat] mean and std.dev: (809.70, 833.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (123.09, 44.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (307, 625, 1322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3352) [M::mem_pestat] mean and std.dev: (823.42, 741.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4367) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 402, 1478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4052) [M::mem_pestat] mean and std.dev: (801.71, 988.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5339) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 869700 reads in 439.159 CPU sec, 14.014 real sec [M::mem_process_seqs] Processed 868858 reads in 363.179 CPU sec, 11.674 real sec sorting block 0, #reads = 1048958 mem = 536880778 peak = 624463872 [M::process] read 865044 sequences (100000121 bp) in 2.159 CPU sec, 2.176 real sec... [M::mem_process_seqs] Processed 869744 reads in 426.624 CPU sec, 13.682 real sec sorting block 1, #reads = 1051489 mem = 536882122 peak = 913870848 [M::process] read 863040 sequences (100000156 bp) in 2.134 CPU sec, 2.155 real sec... [M::process] read 861536 sequences (100000264 bp) in 1.846 CPU sec, 1.849 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (92, 292626, 60, 88) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (229, 660, 1476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3970) [M::mem_pestat] mean and std.dev: (906.80, 987.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5217) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (124.02, 45.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (199, 595, 1125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2977) [M::mem_pestat] mean and std.dev: (716.64, 649.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3903) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (289, 680, 1404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3634) [M::mem_pestat] mean and std.dev: (878.71, 814.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4749) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 291656, 61, 90) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 362, 1338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3722) [M::mem_pestat] mean and std.dev: (723.55, 777.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4914) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (124.00, 44.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 335) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (219, 645, 1209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3189) [M::mem_pestat] mean and std.dev: (751.64, 737.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4179) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 429, 1019) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2677) [M::mem_pestat] mean and std.dev: (651.55, 604.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3506) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 866356 reads in 307.385 CPU sec, 9.835 real sec sorting block 2, #reads = 1051254 mem = 536881917 peak = 913870848 [M::mem_process_seqs] Processed 865044 reads in 367.066 CPU sec, 11.737 real sec [M::process] read 860270 sequences (100000167 bp) in 1.819 CPU sec, 1.844 real sec... sorting block 3, #reads = 1049716 mem = 536887623 peak = 913870848 [M::process] read 859832 sequences (100000080 bp) in 1.850 CPU sec, 1.852 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 290929, 70, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 396, 906) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2292) [M::mem_pestat] mean and std.dev: (581.44, 542.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2985) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 117, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (125.35, 46.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 343) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (313, 682, 1337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3385) [M::mem_pestat] mean and std.dev: (880.81, 746.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4409) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 464, 1212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3194) [M::mem_pestat] mean and std.dev: (795.12, 779.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4185) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 291473, 85, 90) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 417, 1068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2742) [M::mem_pestat] mean and std.dev: (686.49, 686.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 117, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.61, 45.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (307, 627, 1276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3214) [M::mem_pestat] mean and std.dev: (781.37, 661.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4183) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 553, 1210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3172) [M::mem_pestat] mean and std.dev: (802.59, 750.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4153) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 861536 reads in 304.342 CPU sec, 9.754 real sec sorting block 4, #reads = 1049995 mem = 536883524 peak = 913870848 [M::mem_process_seqs] Processed 863040 reads in 363.185 CPU sec, 11.635 real sec [M::process] read 864096 sequences (100000003 bp) in 2.171 CPU sec, 2.210 real sec... [M::process] read 870370 sequences (100000089 bp) in 1.744 CPU sec, 1.748 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (97, 289991, 77, 89) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 350, 1018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2750) [M::mem_pestat] mean and std.dev: (557.13, 541.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 117, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (125.37, 46.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 343) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (153, 488, 1544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4326) [M::mem_pestat] mean and std.dev: (810.39, 894.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5717) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (281, 523, 1149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2885) [M::mem_pestat] mean and std.dev: (669.42, 549.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3753) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 290061, 74, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 383, 965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2475) [M::mem_pestat] mean and std.dev: (572.38, 539.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3230) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 117, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (125.58, 46.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 343) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (179, 525, 1026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2720) [M::mem_pestat] mean and std.dev: (610.52, 577.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3567) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 470, 1034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2728) [M::mem_pestat] mean and std.dev: (682.60, 648.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3575) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (99, 291272, 65, 81) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 373, 1212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3314) [M::mem_pestat] mean and std.dev: (661.19, 697.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4365) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 116, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.39, 45.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (199, 351, 792) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1978) [M::mem_pestat] mean and std.dev: (468.84, 413.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2571) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 427, 960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2512) [M::mem_pestat] mean and std.dev: (532.63, 455.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3288) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 860270 reads in 438.032 CPU sec, 14.057 real sec sorting block 5, #reads = 1050791 mem = 536883499 peak = 913870848 [M::mem_process_seqs] Processed 859832 reads in 365.585 CPU sec, 11.785 real sec sorting block 6, #reads = 1047352 mem = 536884979 peak = 1008484352 [M::process] read 870020 sequences (100000128 bp) in 1.973 CPU sec, 1.998 real sec... [M::mem_process_seqs] Processed 864096 reads in 428.330 CPU sec, 13.776 real sec sorting block 7, #reads = 1047509 mem = 536886162 peak = 1008484352 [M::process] read 868756 sequences (100000071 bp) in 2.104 CPU sec, 2.147 real sec... [M::process] read 866852 sequences (100000225 bp) in 1.727 CPU sec, 1.733 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 293704, 52, 83) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 703, 1275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3361) [M::mem_pestat] mean and std.dev: (758.95, 657.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4404) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (122.96, 44.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (512, 1331, 2148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5420) [M::mem_pestat] mean and std.dev: (1485.67, 1251.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7056) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 401, 1210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3226) [M::mem_pestat] mean and std.dev: (725.26, 742.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4234) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 293478, 56, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 403, 1359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3681) [M::mem_pestat] mean and std.dev: (800.46, 840.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4842) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (122.71, 44.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (317, 554, 1779) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4703) [M::mem_pestat] mean and std.dev: (1035.07, 995.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6165) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 509, 1065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2837) [M::mem_pestat] mean and std.dev: (635.54, 657.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3723) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 870020 reads in 311.882 CPU sec, 9.980 real sec sorting block 8, #reads = 1050567 mem = 536883776 peak = 1008484352 [M::process] read 864266 sequences (100000163 bp) in 1.914 CPU sec, 1.923 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 292738, 75, 84) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 486, 1475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4029) [M::mem_pestat] mean and std.dev: (847.55, 919.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5306) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (123.81, 45.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (244, 628, 1424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3784) [M::mem_pestat] mean and std.dev: (842.80, 751.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4964) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 495, 1404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3830) [M::mem_pestat] mean and std.dev: (813.70, 848.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5043) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 866852 reads in 190.204 CPU sec, 6.107 real sec sorting block 9, #reads = 1052458 mem = 536886328 peak = 1008484352 [M::mem_process_seqs] Processed 870370 reads in 584.684 CPU sec, 18.747 real sec sorting block 10, #reads = 1054076 mem = 536882179 peak = 1008484352 [M::process] read 862748 sequences (100000255 bp) in 2.324 CPU sec, 2.369 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 293829, 59, 80) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 370, 966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2502) [M::mem_pestat] mean and std.dev: (479.15, 453.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3270) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (123.21, 44.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (201, 631, 1441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3921) [M::mem_pestat] mean and std.dev: (814.95, 763.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5161) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (238, 634, 1216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3172) [M::mem_pestat] mean and std.dev: (738.03, 682.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4150) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 860586 sequences (100000028 bp) in 1.750 CPU sec, 1.750 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 292242, 64, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 455, 1065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2665) [M::mem_pestat] mean and std.dev: (686.51, 621.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 117, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.38, 45.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (195, 531, 1250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3360) [M::mem_pestat] mean and std.dev: (639.24, 600.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4415) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 503, 1041) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2721) [M::mem_pestat] mean and std.dev: (735.51, 698.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3561) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (94, 291053, 53, 90) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 599, 1448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3934) [M::mem_pestat] mean and std.dev: (883.78, 879.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5177) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 117, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.75, 45.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (371, 675, 1287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3119) [M::mem_pestat] mean and std.dev: (818.52, 623.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4035) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 517, 1134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2954) [M::mem_pestat] mean and std.dev: (643.78, 569.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3864) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 868756 reads in 622.029 CPU sec, 19.948 real sec [M::mem_process_seqs] Processed 864266 reads in 362.724 CPU sec, 11.677 real sec sorting block 11, #reads = 1051269 mem = 536881627 peak = 1008484352 [M::process] read 861238 sequences (100000150 bp) in 2.328 CPU sec, 2.394 real sec... [M::mem_process_seqs] Processed 862748 reads in 424.641 CPU sec, 13.645 real sec sorting block 12, #reads = 1052119 mem = 536883443 peak = 1008484352 [M::process] read 861156 sequences (100000041 bp) in 2.183 CPU sec, 2.266 real sec... [M::process] read 869030 sequences (100000140 bp) in 1.650 CPU sec, 1.643 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 290760, 67, 80) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (260, 580, 1313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3419) [M::mem_pestat] mean and std.dev: (697.24, 574.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 117, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (125.45, 46.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 343) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (139, 567, 1175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3247) [M::mem_pestat] mean and std.dev: (773.23, 781.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4283) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 433, 1201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3177) [M::mem_pestat] mean and std.dev: (698.72, 704.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4165) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 290161, 68, 94) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 354, 1335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3685) [M::mem_pestat] mean and std.dev: (774.10, 893.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4860) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 117, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (125.23, 46.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 343) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (275, 679, 1911) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5183) [M::mem_pestat] mean and std.dev: (1121.34, 1208.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6819) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 486, 1388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3756) [M::mem_pestat] mean and std.dev: (907.60, 935.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4940) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 860586 reads in 320.822 CPU sec, 10.261 real sec sorting block 13, #reads = 1048862 mem = 536880407 peak = 1008484352 [M::mem_process_seqs] Processed 861238 reads in 381.345 CPU sec, 12.195 real sec sorting block 14, #reads = 1046385 mem = 536881782 peak = 1008484352 [M::process] read 870142 sequences (100000056 bp) in 2.425 CPU sec, 2.468 real sec... [M::process] read 869252 sequences (100000257 bp) in 1.736 CPU sec, 1.741 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (92, 292718, 78, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 500, 1120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2926) [M::mem_pestat] mean and std.dev: (662.47, 569.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (123.14, 44.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (211, 446, 1381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3721) [M::mem_pestat] mean and std.dev: (714.97, 746.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4891) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (238, 585, 1599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4321) [M::mem_pestat] mean and std.dev: (895.97, 919.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5682) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (92, 290812, 70, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (305, 629, 1562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4076) [M::mem_pestat] mean and std.dev: (957.48, 846.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5333) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 117, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (125.26, 46.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 343) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (190, 492, 1561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4303) [M::mem_pestat] mean and std.dev: (947.45, 1189.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5705) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 484, 1072) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2746) [M::mem_pestat] mean and std.dev: (632.69, 564.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3583) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 869030 reads in 326.720 CPU sec, 10.461 real sec sorting block 15, #reads = 1046770 mem = 536879469 peak = 1008484352 [M::mem_process_seqs] Processed 861156 reads in 385.034 CPU sec, 12.335 real sec [M::process] read 867462 sequences (100000107 bp) in 2.128 CPU sec, 2.179 real sec... [M::process] read 865776 sequences (100000000 bp) in 1.656 CPU sec, 1.662 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 293334, 55, 75) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (275, 592, 1177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2981) [M::mem_pestat] mean and std.dev: (628.44, 537.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3883) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (122.92, 44.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (225, 808, 1576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4278) [M::mem_pestat] mean and std.dev: (1047.44, 1011.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5629) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 426, 1328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3544) [M::mem_pestat] mean and std.dev: (766.04, 832.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4652) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 293189, 56, 87) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 485, 1049) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2845) [M::mem_pestat] mean and std.dev: (645.33, 642.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (123.24, 44.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (196, 422, 1635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4513) [M::mem_pestat] mean and std.dev: (710.43, 994.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5952) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (278, 604, 1699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4541) [M::mem_pestat] mean and std.dev: (972.45, 1018.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5962) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 870142 reads in 322.635 CPU sec, 10.330 real sec sorting block 16, #reads = 1048940 mem = 536881281 peak = 1008484352 [M::mem_process_seqs] Processed 869252 reads in 385.626 CPU sec, 12.346 real sec sorting block 17, #reads = 1049502 mem = 536883109 peak = 1011552256 [M::process] read 864328 sequences (100000011 bp) in 2.510 CPU sec, 2.561 real sec... [M::process] read 862928 sequences (100000120 bp) in 1.654 CPU sec, 1.660 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (89, 293556, 65, 92) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 784, 1758) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4792) [M::mem_pestat] mean and std.dev: (904.89, 891.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6309) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (123.50, 44.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (260, 559, 1573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4199) [M::mem_pestat] mean and std.dev: (797.76, 772.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5512) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 395, 1526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4194) [M::mem_pestat] mean and std.dev: (808.89, 861.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5528) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 292257, 60, 88) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 371, 1042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2654) [M::mem_pestat] mean and std.dev: (541.20, 491.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (124.04, 45.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (298, 585, 1539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4021) [M::mem_pestat] mean and std.dev: (1012.05, 1064.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5269) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 486, 1252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3346) [M::mem_pestat] mean and std.dev: (756.57, 717.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4393) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 867462 reads in 317.533 CPU sec, 10.164 real sec sorting block 18, #reads = 1051686 mem = 536878703 peak = 1011552256 [M::mem_process_seqs] Processed 865776 reads in 378.888 CPU sec, 12.142 real sec sorting block 19, #reads = 1053199 mem = 536886416 peak = 1011552256 [M::process] read 861824 sequences (100000007 bp) in 2.025 CPU sec, 2.081 real sec... [M::process] read 861806 sequences (100000105 bp) in 1.858 CPU sec, 1.860 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 290825, 59, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 561, 1346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3618) [M::mem_pestat] mean and std.dev: (775.23, 708.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 117, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.74, 45.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (144, 407, 1279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3549) [M::mem_pestat] mean and std.dev: (710.11, 768.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4684) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 476, 1173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3093) [M::mem_pestat] mean and std.dev: (736.00, 739.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4053) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 292237, 65, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 517, 1238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3376) [M::mem_pestat] mean and std.dev: (686.54, 556.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 116, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.41, 45.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (266, 797, 1379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3605) [M::mem_pestat] mean and std.dev: (816.93, 682.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4718) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 461, 1225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3259) [M::mem_pestat] mean and std.dev: (728.29, 670.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4276) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 862928 reads in 315.968 CPU sec, 10.110 real sec sorting block 20, #reads = 1050372 mem = 536888572 peak = 1011552256 [M::mem_process_seqs] Processed 864328 reads in 374.845 CPU sec, 11.991 real sec [M::process] read 862326 sequences (100000182 bp) in 2.501 CPU sec, 2.541 real sec... sorting block 21, #reads = 1049659 mem = 536891448 peak = 1011552256 [M::process] read 870252 sequences (100000272 bp) in 1.709 CPU sec, 1.712 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 291472, 57, 78) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (242, 396, 1019) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2573) [M::mem_pestat] mean and std.dev: (589.74, 503.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3350) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 117, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.82, 45.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (176, 637, 1504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4160) [M::mem_pestat] mean and std.dev: (925.16, 915.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5488) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 589, 1348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3602) [M::mem_pestat] mean and std.dev: (775.06, 738.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4729) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 289935, 52, 86) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 526, 1403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3859) [M::mem_pestat] mean and std.dev: (855.73, 901.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5087) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 117, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.83, 45.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (167, 526, 928) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2450) [M::mem_pestat] mean and std.dev: (537.56, 468.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3211) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 388, 862) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2268) [M::mem_pestat] mean and std.dev: (529.92, 503.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2971) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 861806 reads in 310.831 CPU sec, 9.948 real sec [M::mem_process_seqs] Processed 861824 reads in 368.539 CPU sec, 11.798 real sec sorting block 22, #reads = 1049856 mem = 536887499 peak = 1011552256 [M::process] read 869612 sequences (100000158 bp) in 2.682 CPU sec, 2.749 real sec... [M::process] read 867972 sequences (100000051 bp) in 1.720 CPU sec, 1.713 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 290583, 68, 93) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 493, 1158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3010) [M::mem_pestat] mean and std.dev: (678.88, 645.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3936) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 117, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.81, 45.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (300, 900, 1735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4605) [M::mem_pestat] mean and std.dev: (953.82, 890.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6040) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 436, 1472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3952) [M::mem_pestat] mean and std.dev: (816.70, 910.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5192) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 292856, 62, 78) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (292, 513, 1431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3709) [M::mem_pestat] mean and std.dev: (805.20, 802.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4848) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (122.90, 44.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (174, 687, 1431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3945) [M::mem_pestat] mean and std.dev: (883.52, 918.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5202) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 499, 1105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2941) [M::mem_pestat] mean and std.dev: (686.18, 679.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3859) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 862326 reads in 313.927 CPU sec, 10.038 real sec sorting block 23, #reads = 1047596 mem = 536879602 peak = 1011552256 [M::mem_process_seqs] Processed 870252 reads in 375.996 CPU sec, 12.021 real sec sorting block 24, #reads = 1045955 mem = 536891918 peak = 1014018048 [M::process] read 866240 sequences (100000233 bp) in 2.411 CPU sec, 2.449 real sec... [M::process] read 865746 sequences (100000120 bp) in 1.504 CPU sec, 1.507 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 293320, 54, 79) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 586, 1285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3467) [M::mem_pestat] mean and std.dev: (743.49, 685.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (123.06, 44.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (149, 341, 923) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2471) [M::mem_pestat] mean and std.dev: (447.94, 459.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3245) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 429, 1054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2820) [M::mem_pestat] mean and std.dev: (713.34, 744.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3703) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 293598, 55, 72) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 409, 1033) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2741) [M::mem_pestat] mean and std.dev: (613.70, 573.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (123.37, 44.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (190, 282, 777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1951) [M::mem_pestat] mean and std.dev: (410.13, 392.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2538) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (266, 493, 1505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3983) [M::mem_pestat] mean and std.dev: (871.93, 962.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5222) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 869612 reads in 310.182 CPU sec, 9.938 real sec sorting block 25, #reads = 1051486 mem = 536886301 peak = 1014018048 [M::mem_process_seqs] Processed 867972 reads in 370.521 CPU sec, 11.871 real sec [M::process] read 864244 sequences (100000180 bp) in 2.555 CPU sec, 2.604 real sec... sorting block 26, #reads = 1053207 mem = 536882373 peak = 1014018048 [M::process] read 864126 sequences (100000062 bp) in 1.767 CPU sec, 1.776 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (89, 292283, 65, 79) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 468, 1238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3332) [M::mem_pestat] mean and std.dev: (652.37, 613.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (124.04, 45.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (206, 744, 1562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4274) [M::mem_pestat] mean and std.dev: (851.02, 762.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5630) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (258, 500, 1143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2913) [M::mem_pestat] mean and std.dev: (684.14, 630.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3798) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 292689, 54, 96) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 526, 1452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4004) [M::mem_pestat] mean and std.dev: (834.44, 924.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5280) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (123.99, 45.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (263, 519, 925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2249) [M::mem_pestat] mean and std.dev: (535.89, 443.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2911) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 378, 1133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2993) [M::mem_pestat] mean and std.dev: (651.54, 679.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3923) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 865746 reads in 312.897 CPU sec, 10.011 real sec [M::mem_process_seqs] Processed 866240 reads in 371.053 CPU sec, 11.882 real sec sorting block 27, #reads = 1048703 mem = 536880630 peak = 1014018048 [M::process] read 862860 sequences (100000172 bp) in 2.040 CPU sec, 2.093 real sec... [M::process] read 863216 sequences (100000002 bp) in 1.614 CPU sec, 1.624 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (86, 291329, 67, 88) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (255, 567, 1202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3096) [M::mem_pestat] mean and std.dev: (752.39, 671.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4043) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 116, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.40, 45.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (392, 827, 1448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3560) [M::mem_pestat] mean and std.dev: (949.36, 812.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4616) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (254, 555, 1488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3956) [M::mem_pestat] mean and std.dev: (813.25, 753.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5190) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 291270, 57, 86) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (291, 540, 1459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3795) [M::mem_pestat] mean and std.dev: (918.16, 894.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4963) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 116, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.47, 45.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (211, 639, 1643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4507) [M::mem_pestat] mean and std.dev: (767.94, 782.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5939) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (246, 485, 1174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3030) [M::mem_pestat] mean and std.dev: (703.24, 650.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3958) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 864244 reads in 314.481 CPU sec, 10.071 real sec sorting block 28, #reads = 1050589 mem = 536882031 peak = 1014018048 [M::mem_process_seqs] Processed 864126 reads in 375.622 CPU sec, 12.026 real sec sorting block 29, #reads = 1048467 mem = 536878880 peak = 1099386880 [M::process] read 865002 sequences (100000159 bp) in 2.960 CPU sec, 3.006 real sec... [M::process] read 874018 sequences (100000007 bp) in 2.170 CPU sec, 2.162 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 291362, 82, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 566, 2055) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5727) [M::mem_pestat] mean and std.dev: (1221.08, 1421.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 117, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.71, 45.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (168, 397, 1046) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2802) [M::mem_pestat] mean and std.dev: (584.37, 605.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3680) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 582, 1905) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5345) [M::mem_pestat] mean and std.dev: (991.56, 1057.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7065) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 291477, 63, 73) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 429, 1156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3114) [M::mem_pestat] mean and std.dev: (623.81, 588.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4093) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 117, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.55, 45.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (242, 583, 2060) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5696) [M::mem_pestat] mean and std.dev: (1187.08, 1415.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7514) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (250, 540, 1270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3310) [M::mem_pestat] mean and std.dev: (804.34, 690.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4330) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 862860 reads in 308.738 CPU sec, 9.856 real sec sorting block 30, #reads = 1046746 mem = 536885404 peak = 1099386880 [M::mem_process_seqs] Processed 863216 reads in 375.849 CPU sec, 12.011 real sec [M::process] read 871876 sequences (100000012 bp) in 2.192 CPU sec, 2.242 real sec... sorting block 31, #reads = 1048240 mem = 536890558 peak = 1099386880 [M::process] read 871184 sequences (100000058 bp) in 2.256 CPU sec, 2.265 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 290800, 53, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 681, 1313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3587) [M::mem_pestat] mean and std.dev: (644.07, 537.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 116, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.30, 45.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (235, 463, 1244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3262) [M::mem_pestat] mean and std.dev: (605.72, 625.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4271) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (248, 481, 1133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2903) [M::mem_pestat] mean and std.dev: (700.03, 657.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3788) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 293555, 47, 77) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (296, 480, 1281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3251) [M::mem_pestat] mean and std.dev: (802.07, 741.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4236) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (122.56, 43.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (287, 637, 1298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3320) [M::mem_pestat] mean and std.dev: (792.83, 679.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4331) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 436, 1077) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2767) [M::mem_pestat] mean and std.dev: (682.00, 593.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3612) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 294209, 59, 72) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 421, 842) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2192) [M::mem_pestat] mean and std.dev: (444.40, 338.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2867) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (89, 115, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 269) [M::mem_pestat] mean and std.dev: (121.99, 43.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (241, 518, 1308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3442) [M::mem_pestat] mean and std.dev: (823.38, 770.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4509) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 419, 1336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3682) [M::mem_pestat] mean and std.dev: (822.85, 847.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4855) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 865002 reads in 509.339 CPU sec, 16.344 real sec sorting block 32, #reads = 1044503 mem = 536882414 peak = 1099386880 [M::mem_process_seqs] Processed 871876 reads in 430.204 CPU sec, 13.860 real sec [M::process] read 869256 sequences (100000181 bp) in 2.501 CPU sec, 2.553 real sec... [M::mem_process_seqs] Processed 874018 reads in 504.997 CPU sec, 16.243 real sec sorting block 33, #reads = 1048134 mem = 536883641 peak = 1099386880 [M::process] read 868204 sequences (100000082 bp) in 2.368 CPU sec, 2.419 real sec... [M::process] read 868418 sequences (100000106 bp) in 2.106 CPU sec, 2.112 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 294216, 49, 97) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 567, 1339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3715) [M::mem_pestat] mean and std.dev: (740.31, 787.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4903) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (122.63, 43.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (263, 585, 1867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5075) [M::mem_pestat] mean and std.dev: (925.24, 976.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6679) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (265, 533, 934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2272) [M::mem_pestat] mean and std.dev: (593.73, 489.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2941) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 293126, 61, 85) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 412, 962) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2522) [M::mem_pestat] mean and std.dev: (579.41, 562.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3302) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (123.24, 44.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (264, 590, 1179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3009) [M::mem_pestat] mean and std.dev: (685.33, 592.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3924) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 370, 993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2561) [M::mem_pestat] mean and std.dev: (620.81, 656.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3345) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 293764, 70, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 472, 989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2565) [M::mem_pestat] mean and std.dev: (567.24, 441.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3353) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (122.81, 43.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (190, 674, 1314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3562) [M::mem_pestat] mean and std.dev: (883.85, 832.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4686) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 339, 1533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4243) [M::mem_pestat] mean and std.dev: (761.20, 877.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5598) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 871184 reads in 509.251 CPU sec, 16.326 real sec sorting block 34, #reads = 1048644 mem = 536880559 peak = 1099386880 [M::mem_process_seqs] Processed 868204 reads in 432.115 CPU sec, 13.911 real sec sorting block 35, #reads = 1050057 mem = 536898449 peak = 1107308544 [M::process] read 865794 sequences (100000057 bp) in 2.794 CPU sec, 2.836 real sec... [M::mem_process_seqs] Processed 869256 reads in 506.620 CPU sec, 16.277 real sec sorting block 36, #reads = 1048571 mem = 536887341 peak = 1107308544 [M::process] read 862644 sequences (100000246 bp) in 2.290 CPU sec, 2.335 real sec... [M::process] read 862252 sequences (100000091 bp) in 2.153 CPU sec, 2.156 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 291970, 68, 78) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 398, 868) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2340) [M::mem_pestat] mean and std.dev: (555.81, 503.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3076) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (124.04, 44.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 335) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (218, 890, 1579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4301) [M::mem_pestat] mean and std.dev: (893.29, 798.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5662) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (281, 634, 1506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3956) [M::mem_pestat] mean and std.dev: (864.32, 785.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5181) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 293252, 62, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (276, 653, 1403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3657) [M::mem_pestat] mean and std.dev: (814.11, 753.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4784) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (123.30, 44.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (204, 516, 1167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3093) [M::mem_pestat] mean and std.dev: (665.16, 645.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4056) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (248, 491, 865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2099) [M::mem_pestat] mean and std.dev: (540.00, 444.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2716) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 865794 reads in 366.336 CPU sec, 11.715 real sec sorting block 37, #reads = 1048493 mem = 536889109 peak = 1107308544 [M::mem_process_seqs] Processed 868418 reads in 437.282 CPU sec, 13.983 real sec [M::process] read 876322 sequences (100000131 bp) in 2.949 CPU sec, 2.980 real sec... sorting block 38, #reads = 1047682 mem = 536888656 peak = 1107308544 [M::process] read 876570 sequences (100000224 bp) in 2.232 CPU sec, 2.237 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (91, 289734, 70, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 504, 985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2587) [M::mem_pestat] mean and std.dev: (613.64, 538.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 117, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.63, 45.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (314, 875, 1256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3140) [M::mem_pestat] mean and std.dev: (795.27, 628.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4082) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 545, 1137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2967) [M::mem_pestat] mean and std.dev: (690.44, 639.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3882) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (102, 288905, 73, 80) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 576, 1343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3599) [M::mem_pestat] mean and std.dev: (669.90, 605.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 117, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.74, 45.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (204, 663, 1680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4632) [M::mem_pestat] mean and std.dev: (842.67, 902.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6108) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 496, 1247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3367) [M::mem_pestat] mean and std.dev: (773.40, 743.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4427) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 862644 reads in 368.060 CPU sec, 11.771 real sec [M::mem_process_seqs] Processed 862252 reads in 442.346 CPU sec, 14.152 real sec sorting block 39, #reads = 1041801 mem = 536895159 peak = 1107308544 [M::process] read 871700 sequences (100000015 bp) in 2.260 CPU sec, 2.299 real sec... [M::process] read 870254 sequences (100000064 bp) in 2.288 CPU sec, 2.295 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 294384, 54, 77) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 400, 1078) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2904) [M::mem_pestat] mean and std.dev: (525.67, 523.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3817) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (89, 114, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 266) [M::mem_pestat] mean and std.dev: (121.34, 43.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 325) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (245, 621, 1598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4304) [M::mem_pestat] mean and std.dev: (915.98, 925.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5657) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (277, 682, 1042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2572) [M::mem_pestat] mean and std.dev: (704.70, 493.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3337) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (91, 293359, 48, 77) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 570, 1216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3262) [M::mem_pestat] mean and std.dev: (698.73, 695.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4285) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (89, 114, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 266) [M::mem_pestat] mean and std.dev: (121.37, 43.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 325) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (182, 594, 1514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4178) [M::mem_pestat] mean and std.dev: (727.81, 704.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5510) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 501, 1590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4346) [M::mem_pestat] mean and std.dev: (902.07, 975.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5724) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 876570 reads in 422.099 CPU sec, 13.537 real sec sorting block 40, #reads = 1037299 mem = 536882803 peak = 1116508160 [M::mem_process_seqs] Processed 876322 reads in 510.909 CPU sec, 16.391 real sec sorting block 41, #reads = 1038250 mem = 536884077 peak = 1116508160 [M::process] read 869672 sequences (100000196 bp) in 2.816 CPU sec, 2.879 real sec... [M::process] read 867922 sequences (100000083 bp) in 2.321 CPU sec, 2.342 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 293838, 49, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 376, 1193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3181) [M::mem_pestat] mean and std.dev: (629.50, 686.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4175) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (122.52, 43.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (256, 525, 2218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6142) [M::mem_pestat] mean and std.dev: (992.30, 1107.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8104) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 484, 1316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3532) [M::mem_pestat] mean and std.dev: (783.19, 753.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4640) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 293137, 59, 75) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 422, 1195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3189) [M::mem_pestat] mean and std.dev: (580.13, 636.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4186) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (122.78, 43.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (423, 994, 3005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8169) [M::mem_pestat] mean and std.dev: (1822.49, 2163.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10751) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 576, 1621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4425) [M::mem_pestat] mean and std.dev: (881.46, 863.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5827) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 871700 reads in 435.865 CPU sec, 13.980 real sec sorting block 42, #reads = 1033927 mem = 536878651 peak = 1116508160 [M::mem_process_seqs] Processed 870254 reads in 528.597 CPU sec, 16.951 real sec [M::process] read 866398 sequences (100000030 bp) in 2.351 CPU sec, 2.380 real sec... sorting block 43, #reads = 1040937 mem = 536886342 peak = 1116508160 [M::process] read 863854 sequences (100000151 bp) in 2.349 CPU sec, 2.359 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 293074, 48, 89) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 382, 773) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1957) [M::mem_pestat] mean and std.dev: (486.38, 431.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (123.08, 44.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (227, 611, 1417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3797) [M::mem_pestat] mean and std.dev: (797.39, 736.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4987) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 466, 961) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2433) [M::mem_pestat] mean and std.dev: (592.64, 493.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3169) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 292271, 47, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 414, 1493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4073) [M::mem_pestat] mean and std.dev: (845.08, 834.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (123.41, 44.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (185, 455, 1832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5126) [M::mem_pestat] mean and std.dev: (1005.06, 1162.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6773) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 502, 1142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2976) [M::mem_pestat] mean and std.dev: (693.46, 674.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3893) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 869672 reads in 405.995 CPU sec, 13.024 real sec sorting block 44, #reads = 1039241 mem = 536881507 peak = 1116508160 [M::mem_process_seqs] Processed 867922 reads in 489.901 CPU sec, 15.723 real sec [M::process] read 862812 sequences (100000046 bp) in 2.934 CPU sec, 2.981 real sec... [M::process] read 873736 sequences (100000192 bp) in 2.083 CPU sec, 2.089 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 290916, 56, 95) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 364, 1251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3447) [M::mem_pestat] mean and std.dev: (574.64, 689.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (124.15, 44.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 335) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (274, 513, 1224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3124) [M::mem_pestat] mean and std.dev: (664.43, 687.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4074) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 450, 1054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2802) [M::mem_pestat] mean and std.dev: (603.69, 596.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3676) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (89, 292077, 72, 75) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (246, 469, 1222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3174) [M::mem_pestat] mean and std.dev: (691.01, 642.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4150) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (123.87, 44.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (262, 657, 1662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4462) [M::mem_pestat] mean and std.dev: (962.60, 950.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5862) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 572, 1543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4269) [M::mem_pestat] mean and std.dev: (890.71, 944.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5632) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 863854 reads in 394.834 CPU sec, 12.657 real sec sorting block 45, #reads = 1044602 mem = 536883542 peak = 1116508160 [M::mem_process_seqs] Processed 866398 reads in 473.318 CPU sec, 15.185 real sec sorting block 46, #reads = 1044821 mem = 536884940 peak = 1147985920 [M::process] read 872706 sequences (100000089 bp) in 2.546 CPU sec, 2.589 real sec... [M::process] read 870360 sequences (100000117 bp) in 2.039 CPU sec, 2.050 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 289738, 74, 95) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 356, 936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2474) [M::mem_pestat] mean and std.dev: (486.77, 424.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3243) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 117, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.50, 45.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (278, 643, 1628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4328) [M::mem_pestat] mean and std.dev: (939.30, 983.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5678) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 467, 1668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4652) [M::mem_pestat] mean and std.dev: (772.78, 830.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6144) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (87, 292895, 63, 100) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 493, 1332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3672) [M::mem_pestat] mean and std.dev: (653.36, 599.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4842) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (89, 114, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 269) [M::mem_pestat] mean and std.dev: (122.03, 43.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (358, 743, 2057) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5455) [M::mem_pestat] mean and std.dev: (1203.63, 1209.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7154) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 536, 1449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3861) [M::mem_pestat] mean and std.dev: (741.34, 763.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5067) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 294471, 57, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 471, 1313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3579) [M::mem_pestat] mean and std.dev: (721.94, 696.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4712) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (122.32, 43.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (368, 626, 1941) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5087) [M::mem_pestat] mean and std.dev: (1029.47, 966.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6660) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 486, 1721) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4721) [M::mem_pestat] mean and std.dev: (770.18, 902.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6221) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 862812 reads in 565.407 CPU sec, 18.159 real sec sorting block 47, #reads = 1044252 mem = 536879327 peak = 1147985920 [M::mem_process_seqs] Processed 873736 reads in 487.821 CPU sec, 15.720 real sec sorting block 48, #reads = 1030954 mem = 536882092 peak = 1147985920 [M::process] read 869246 sequences (100000168 bp) in 2.911 CPU sec, 2.944 real sec... [M::mem_process_seqs] Processed 872706 reads in 568.393 CPU sec, 18.288 real sec sorting block 49, #reads = 1048283 mem = 536881172 peak = 1147985920 [M::process] read 868780 sequences (100000277 bp) in 2.701 CPU sec, 2.770 real sec... [M::process] read 867588 sequences (100000222 bp) in 2.168 CPU sec, 2.179 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 293994, 50, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 428, 836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2088) [M::mem_pestat] mean and std.dev: (478.96, 401.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (122.72, 43.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (158, 584, 1278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3518) [M::mem_pestat] mean and std.dev: (766.77, 751.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4638) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 547, 1829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5135) [M::mem_pestat] mean and std.dev: (990.32, 1045.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6788) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 293331, 49, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 514, 1758) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4850) [M::mem_pestat] mean and std.dev: (1004.57, 1220.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (123.17, 44.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (207, 878, 1627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4467) [M::mem_pestat] mean and std.dev: (922.28, 842.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5887) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 349, 1189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3235) [M::mem_pestat] mean and std.dev: (688.47, 764.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4258) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 870360 reads in 385.803 CPU sec, 12.347 real sec sorting block 50, #reads = 1052690 mem = 536878142 peak = 1147985920 [M::mem_process_seqs] Processed 869246 reads in 462.678 CPU sec, 14.825 real sec [M::process] read 865530 sequences (100000064 bp) in 2.529 CPU sec, 2.581 real sec... [M::process] read 864736 sequences (100000047 bp) in 2.232 CPU sec, 2.238 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 293192, 70, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (229, 592, 1916) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5290) [M::mem_pestat] mean and std.dev: (1017.00, 1110.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6977) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (123.22, 44.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (128, 583, 1628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4628) [M::mem_pestat] mean and std.dev: (930.75, 1073.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6128) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (326, 607, 1409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3575) [M::mem_pestat] mean and std.dev: (854.84, 757.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4658) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 291988, 62, 91) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 368, 1210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3338) [M::mem_pestat] mean and std.dev: (760.09, 795.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (123.49, 44.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (211, 692, 1238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3292) [M::mem_pestat] mean and std.dev: (822.58, 770.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4319) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (317, 609, 1369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3473) [M::mem_pestat] mean and std.dev: (871.02, 787.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4525) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 868780 reads in 396.933 CPU sec, 12.709 real sec sorting block 51, #reads = 1048245 mem = 536889850 peak = 1147985920 [M::mem_process_seqs] Processed 867588 reads in 476.068 CPU sec, 15.263 real sec sorting block 52, #reads = 1045778 mem = 536881176 peak = 1147985920 [M::process] read 864786 sequences (100000075 bp) in 2.585 CPU sec, 2.642 real sec... [M::process] read 869892 sequences (100000015 bp) in 2.283 CPU sec, 2.293 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (91, 291680, 66, 93) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 549, 1113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2909) [M::mem_pestat] mean and std.dev: (614.41, 571.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3807) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (124.02, 45.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (209, 506, 1097) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2873) [M::mem_pestat] mean and std.dev: (634.51, 573.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3761) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (293, 616, 1107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2735) [M::mem_pestat] mean and std.dev: (746.01, 635.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3549) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 291020, 51, 88) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (257, 856, 1610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4316) [M::mem_pestat] mean and std.dev: (931.22, 834.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (124.00, 44.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 335) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (179, 415, 1260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3422) [M::mem_pestat] mean and std.dev: (689.13, 760.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4503) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 442, 1369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3713) [M::mem_pestat] mean and std.dev: (782.71, 878.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4885) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 865530 reads in 393.994 CPU sec, 12.619 real sec sorting block 53, #reads = 1046382 mem = 536882800 peak = 1147985920 [M::mem_process_seqs] Processed 864736 reads in 473.936 CPU sec, 15.194 real sec sorting block 54, #reads = 1044531 mem = 536890125 peak = 1147985920 [M::process] read 878714 sequences (100000017 bp) in 2.627 CPU sec, 2.666 real sec... [M::process] read 875126 sequences (100000070 bp) in 2.255 CPU sec, 2.263 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 290978, 42, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 359, 1366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3748) [M::mem_pestat] mean and std.dev: (618.56, 651.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4939) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (124.06, 44.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 335) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (167, 540, 1242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3392) [M::mem_pestat] mean and std.dev: (682.58, 721.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4467) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 657, 1430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3864) [M::mem_pestat] mean and std.dev: (845.21, 783.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5081) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (90, 290925, 71, 87) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 596, 1485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4141) [M::mem_pestat] mean and std.dev: (876.92, 919.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (122.66, 43.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (405, 700, 1845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4725) [M::mem_pestat] mean and std.dev: (1038.72, 1027.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6165) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 549, 1286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3408) [M::mem_pestat] mean and std.dev: (761.04, 731.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4469) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 864786 reads in 417.711 CPU sec, 13.382 real sec sorting block 55, #reads = 1040569 mem = 536886375 peak = 1147985920 [M::mem_process_seqs] Processed 869892 reads in 512.336 CPU sec, 16.402 real sec [M::process] read 871854 sequences (100000162 bp) in 2.839 CPU sec, 2.876 real sec... sorting block 56, #reads = 1024859 mem = 536879949 peak = 1147985920 [M::process] read 871030 sequences (100000013 bp) in 2.366 CPU sec, 2.376 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 294670, 60, 75) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 375, 974) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2518) [M::mem_pestat] mean and std.dev: (564.86, 521.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3290) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (89, 114, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 266) [M::mem_pestat] mean and std.dev: (120.91, 42.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 325) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (178, 536, 1239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3361) [M::mem_pestat] mean and std.dev: (665.07, 711.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4422) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 331, 1316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3656) [M::mem_pestat] mean and std.dev: (726.50, 833.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4826) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 294133, 58, 78) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 359, 1044) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2796) [M::mem_pestat] mean and std.dev: (599.29, 632.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (89, 114, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 269) [M::mem_pestat] mean and std.dev: (121.72, 43.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (204, 538, 1245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3327) [M::mem_pestat] mean and std.dev: (727.07, 717.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4368) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 386, 980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2558) [M::mem_pestat] mean and std.dev: (616.49, 609.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3347) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 878714 reads in 429.597 CPU sec, 13.775 real sec [M::mem_process_seqs] Processed 875126 reads in 518.067 CPU sec, 16.617 real sec sorting block 57, #reads = 1028882 mem = 536890624 peak = 1147985920 [M::process] read 867662 sequences (100000081 bp) in 2.524 CPU sec, 2.577 real sec... [M::process] read 866530 sequences (100000176 bp) in 2.041 CPU sec, 2.048 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 292694, 69, 87) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 464, 1090) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2932) [M::mem_pestat] mean and std.dev: (698.63, 719.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3853) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (122.65, 44.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (275, 958, 2792) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7826) [M::mem_pestat] mean and std.dev: (1554.90, 1701.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10343) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 603, 1436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3936) [M::mem_pestat] mean and std.dev: (784.15, 742.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5186) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 293326, 69, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 450, 1585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4333) [M::mem_pestat] mean and std.dev: (711.61, 729.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (122.42, 43.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (222, 696, 2346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6594) [M::mem_pestat] mean and std.dev: (1153.61, 1295.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8718) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (293, 686, 1821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4877) [M::mem_pestat] mean and std.dev: (1163.20, 1174.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6405) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 871030 reads in 407.090 CPU sec, 13.055 real sec sorting block 58, #reads = 1038660 mem = 536884578 peak = 1190621184 [M::mem_process_seqs] Processed 871854 reads in 492.195 CPU sec, 15.792 real sec sorting block 59, #reads = 1037930 mem = 536881605 peak = 1190621184 [M::process] read 865472 sequences (100000093 bp) in 2.817 CPU sec, 2.857 real sec... [M::process] read 864954 sequences (100000126 bp) in 2.304 CPU sec, 2.309 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 291920, 65, 73) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 438, 1229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3319) [M::mem_pestat] mean and std.dev: (677.33, 691.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4364) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (123.39, 44.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (232, 444, 1025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2611) [M::mem_pestat] mean and std.dev: (642.60, 632.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3404) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (246, 443, 1018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2562) [M::mem_pestat] mean and std.dev: (590.41, 544.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3334) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (97, 291208, 71, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (244, 487, 1197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3103) [M::mem_pestat] mean and std.dev: (707.44, 647.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4056) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (123.74, 44.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (191, 585, 1331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3611) [M::mem_pestat] mean and std.dev: (764.54, 790.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4751) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 445, 961) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2445) [M::mem_pestat] mean and std.dev: (578.31, 496.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3187) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 867662 reads in 404.254 CPU sec, 12.954 real sec sorting block 60, #reads = 1039880 mem = 536898683 peak = 1190621184 [M::mem_process_seqs] Processed 866530 reads in 485.848 CPU sec, 15.572 real sec [M::process] read 842320 sequences (97403993 bp) in 2.852 CPU sec, 2.883 real sec... sorting block 61, #reads = 1042294 mem = 536877830 peak = 1190621184 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 291781, 58, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 425, 1095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2929) [M::mem_pestat] mean and std.dev: (609.80, 550.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3846) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (124.09, 45.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (206, 525, 1195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3173) [M::mem_pestat] mean and std.dev: (769.14, 796.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4162) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (316, 848, 1725) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4543) [M::mem_pestat] mean and std.dev: (1041.96, 936.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5952) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 291195, 60, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 413, 951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2397) [M::mem_pestat] mean and std.dev: (484.18, 414.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3120) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (124.00, 44.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 335) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (234, 581, 1369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3639) [M::mem_pestat] mean and std.dev: (864.55, 868.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4774) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 489, 1292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3478) [M::mem_pestat] mean and std.dev: (682.39, 653.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4571) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 865472 reads in 396.811 CPU sec, 12.782 real sec sorting block 62, #reads = 1043010 mem = 536883431 peak = 1190621184 [M::mem_process_seqs] Processed 864954 reads in 473.620 CPU sec, 15.287 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 283852, 76, 78) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 473, 1106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2958) [M::mem_pestat] mean and std.dev: (622.27, 573.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3884) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (124.21, 45.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 335) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (282, 990, 2677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7467) [M::mem_pestat] mean and std.dev: (1545.11, 1621.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9862) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (257, 563, 1014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2528) [M::mem_pestat] mean and std.dev: (610.64, 482.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3285) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 842320 reads in 229.324 CPU sec, 7.469 real sec sorting block 63, #reads = 1046073 mem = 536888500 peak = 1190621184 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: 1f8bfbff fffab20b extended: f1bf07ab 1a407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8559C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 65878784 overall: 77470355456 mem 17261.835 user 111.048 sys 566.066 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1029_BCAN-T1-TDNA-1_A23MHWWLT4_1.L003\tPU:L003\tSM:1029_BCAN-T1-TDNA-1_A23MHWWLT4_1\tLB:1029_BCAN-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1029_BCAN-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 1029_BCAN-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 566.099 sec; CPU: 17372.885 sec sorting block 64, #reads = 786680 mem = 404494753 peak = 1193979904 uniq header count 1 merging 65 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: 1f8bfbff fffab20b extended: f1bf07ab 1a407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8559C threads: 31 max 64 algo: util-sort license: sentieon:util=1 output file size: 4958700945 output reads: 67740687 bam_mem_sort: 65 calls 27.829 user 0.107 sys 28.190 real bam_write: 65 calls 30.142 user 1.061 sys 37.827 real execute: 1 calls 18.908 user 0.468 sys 596.127 real merge_files: 1 calls 18.884 user 0.445 sys 29.693 real parse_chunk: 1037 calls 49.439 user 11.545 sys 62.559 real read_chunk: 2872 calls 23.038 user 15.815 sys 521.912 real sort_block: 1 calls 13.735 user 15.327 sys 509.677 real write_chunk: 1833 calls 18.456 user 0.298 sys 28.833 real overall: 4398215168 mem 1113.934 user 37.960 sys 596.149 real ls: cannot access '*.cram': No such file or directory