Detecting adapter sequence for read1...
>Illumina TruSeq Adapter Read 1
AGATCGGAAGAGCACACGTCTGAACTCCAGTCA

Detecting adapter sequence for read2...
>Illumina TruSeq Adapter Read 2
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT

Read1 before filtering:
total reads: 131073082
total bases: 19792035382
Q20 bases: 19246983903(97.2461%)
Q30 bases: 18209812548(92.0058%)
Q40 bases: 18209812548(92.0058%)

Read2 before filtering:
total reads: 131073082
total bases: 19792035382
Q20 bases: 18708688588(94.5263%)
Q30 bases: 16939529378(85.5876%)
Q40 bases: 16939529378(85.5876%)

Read1 after filtering:
total reads: 128062561
total bases: 18742373752
Q20 bases: 18320022223(97.7465%)
Q30 bases: 17412758779(92.9058%)
Q40 bases: 17412758779(92.9058%)

Read2 after filtering:
total reads: 128062561
total bases: 18743767487
Q20 bases: 17920916338(95.61%)
Q30 bases: 16373010242(87.3518%)
Q40 bases: 16373010242(87.3518%)

Filtering result:
reads passed filter: 256125122
reads failed due to low quality: 5998378
reads failed due to too many N: 15936
reads failed due to too short: 6728
reads with adapter trimmed: 32303665
bases trimmed due to adapters: 1193558779

Duplication rate: 12.6809%

Insert size peak (evaluated by paired-end reads): 228

JSON report: 1029_N5D-T1-TDNA-1_A23MHWWLT4_1.fastp.json
HTML report: 1029_N5D-T1-TDNA-1_A23MHWWLT4_1.fastp.html

fastp --in1 1029_N5D-T1-TDNA-1_A23MHWWLT4_1_S1_L003_R1_001.fastq.gz --in2 1029_N5D-T1-TDNA-1_A23MHWWLT4_1_S1_L003_R2_001.fastq.gz --out1 1029_N5D-T1-TDNA-1_A23MHWWLT4_1/1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz --out2 1029_N5D-T1-TDNA-1_A23MHWWLT4_1/1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz --detect_adapter_for_pe --length_required 15 --json 1029_N5D-T1-TDNA-1_A23MHWWLT4_1.fastp.json --html 1029_N5D-T1-TDNA-1_A23MHWWLT4_1.fastp.html --thread 4 --split_by_lines 400000000 
fastp v1.0.1, time used: 1630 seconds