cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_86S-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1933.886 CPU sec, 70.164 real sec [M::process] read 706512 sequences (100000277 bp) in 1.072 CPU sec, 1.076 real sec... [M::process] read 703088 sequences (100000136 bp) in 1.388 CPU sec, 1.461 real sec... [M::process] read 702292 sequences (100000288 bp) in 1.478 CPU sec, 1.481 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 291970, 25, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 249, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (249.59, 133.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 221, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (245.96, 123.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (372, 927, 1995) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5241) [M::mem_pestat] mean and std.dev: (1133.35, 1160.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6864) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 379, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1218) [M::mem_pestat] mean and std.dev: (340.95, 235.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1558) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701926 sequences (100000264 bp) in 1.149 CPU sec, 1.148 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 290502, 21, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 289, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006) [M::mem_pestat] mean and std.dev: (272.65, 148.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1284) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 233, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (258.78, 130.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (332, 469, 1407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3557) [M::mem_pestat] mean and std.dev: (709.32, 622.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4632) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 244, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (276.44, 146.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 290114, 28, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 233, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1120) [M::mem_pestat] mean and std.dev: (275.89, 163.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 231, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (256.35, 129.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (509, 1057, 1586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3740) [M::mem_pestat] mean and std.dev: (1081.43, 795.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4817) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 279, 1014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2738) [M::mem_pestat] mean and std.dev: (528.41, 567.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3600) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 706512 reads in 254.988 CPU sec, 8.192 real sec [M::mem_process_seqs] Processed 702292 reads in 167.891 CPU sec, 5.446 real sec [M::mem_process_seqs] Processed 703088 reads in 183.979 CPU sec, 5.972 real sec sorting block 0, #reads = 1090975 mem = 536879824 peak = 602181632 [M::process] read 701436 sequences (100000084 bp) in 2.378 CPU sec, 2.535 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 290077, 8, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 241, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (212.62, 102.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 235, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (260.60, 131.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 222, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (246.54, 99.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 700920 sequences (100000257 bp) in 1.598 CPU sec, 1.600 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 289887, 15, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 275, 917) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2393) [M::mem_pestat] mean and std.dev: (466.03, 487.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3131) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 236, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (261.86, 131.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (381, 561, 888) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1902) [M::mem_pestat] mean and std.dev: (498.15, 279.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2409) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 272, 603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1489) [M::mem_pestat] mean and std.dev: (348.20, 292.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1932) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701926 reads in 159.173 CPU sec, 5.053 real sec sorting block 1, #reads = 1090397 mem = 536879282 peak = 839684096 [M::process] read 700626 sequences (100000097 bp) in 1.744 CPU sec, 1.763 real sec... [M::mem_process_seqs] Processed 701436 reads in 106.153 CPU sec, 3.348 real sec sorting block 2, #reads = 1089292 mem = 536877651 peak = 877858816 [M::process] read 700154 sequences (100000157 bp) in 2.047 CPU sec, 2.138 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 289959, 21, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 215, 666) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1694) [M::mem_pestat] mean and std.dev: (306.08, 215.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2208) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 238, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (263.27, 132.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (624, 806, 2494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6234) [M::mem_pestat] mean and std.dev: (1255.50, 1336.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8104) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 303, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (293.60, 109.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699354 sequences (100000111 bp) in 1.400 CPU sec, 1.406 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 289396, 12, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 297, 680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1736) [M::mem_pestat] mean and std.dev: (344.54, 307.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2264) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 240, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (264.75, 133.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (148, 838, 3462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10090) [M::mem_pestat] mean and std.dev: (2069.42, 2843.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13442) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 481, 752) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1820) [M::mem_pestat] mean and std.dev: (432.17, 267.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2354) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700920 reads in 157.800 CPU sec, 5.037 real sec [M::mem_process_seqs] Processed 700626 reads in 108.579 CPU sec, 3.490 real sec sorting block 3, #reads = 1088613 mem = 536877964 peak = 877858816 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 289843, 14, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 306, 810) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2056) [M::mem_pestat] mean and std.dev: (431.61, 298.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 241, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (266.18, 134.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (741, 1080, 5319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14475) [M::mem_pestat] mean and std.dev: (3390.43, 3644.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19053) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 410, 1012) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2572) [M::mem_pestat] mean and std.dev: (623.92, 578.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3352) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699458 sequences (100000255 bp) in 2.262 CPU sec, 2.395 real sec... [M::process] read 699094 sequences (100000271 bp) in 1.539 CPU sec, 1.539 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 289587, 21, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 229, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060) [M::mem_pestat] mean and std.dev: (294.16, 175.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1356) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 243, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (267.95, 135.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (295, 977, 4128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11794) [M::mem_pestat] mean and std.dev: (1734.33, 1689.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15627) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 241, 660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1608) [M::mem_pestat] mean and std.dev: (318.12, 293.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2082) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700154 reads in 157.562 CPU sec, 5.018 real sec [M::mem_process_seqs] Processed 699354 reads in 107.772 CPU sec, 3.436 real sec sorting block 4, #reads = 1088700 mem = 536879732 peak = 877858816 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 289350, 16, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 306, 1323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3439) [M::mem_pestat] mean and std.dev: (593.40, 575.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 242, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (268.17, 135.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (140, 412, 633) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1619) [M::mem_pestat] mean and std.dev: (517.44, 541.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2684) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 255, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1129) [M::mem_pestat] mean and std.dev: (252.77, 155.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1457) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698626 sequences (100000103 bp) in 2.262 CPU sec, 2.396 real sec... [M::process] read 699090 sequences (100000094 bp) in 1.610 CPU sec, 1.602 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 289400, 17, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 233, 977) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2607) [M::mem_pestat] mean and std.dev: (385.04, 394.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 243, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (267.89, 134.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (218, 306, 660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1544) [M::mem_pestat] mean and std.dev: (292.00, 131.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1986) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 215, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (221.16, 87.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699458 reads in 161.360 CPU sec, 5.139 real sec sorting block 5, #reads = 1088554 mem = 536879048 peak = 877858816 [M::mem_process_seqs] Processed 699094 reads in 103.921 CPU sec, 3.301 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 289158, 18, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 452, 1436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3942) [M::mem_pestat] mean and std.dev: (1062.24, 1283.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6197) [M::mem_pestat] analyzing insert size distribution for orientation FR... sorting block 6, #reads = 1088884 mem = 536879315 peak = 877858816 [M::mem_pestat] (25, 50, 75) percentile: (162, 244, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (270.59, 136.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (153, 594, 889) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2361) [M::mem_pestat] mean and std.dev: (396.07, 296.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3097) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 307, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (286.00, 104.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698078 sequences (100000293 bp) in 2.778 CPU sec, 2.931 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 288854, 21, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 291, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034) [M::mem_pestat] mean and std.dev: (324.84, 199.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1311) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 244, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (270.86, 137.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (515, 693, 2491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6443) [M::mem_pestat] mean and std.dev: (1089.25, 1192.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8419) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 293, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1184) [M::mem_pestat] mean and std.dev: (314.23, 173.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1524) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697846 sequences (100000078 bp) in 1.820 CPU sec, 1.823 real sec... [M::mem_process_seqs] Processed 698626 reads in 161.398 CPU sec, 5.134 real sec [M::mem_process_seqs] Processed 699090 reads in 104.970 CPU sec, 3.551 real sec sorting block 7, #reads = 1086187 mem = 536881731 peak = 877858816 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 288805, 25, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 308, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1171) [M::mem_pestat] mean and std.dev: (325.46, 188.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 246, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (271.54, 136.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (569, 686, 1538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3476) [M::mem_pestat] mean and std.dev: (842.83, 546.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4445) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (238, 330, 718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1678) [M::mem_pestat] mean and std.dev: (416.88, 295.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2158) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697764 sequences (100000104 bp) in 2.357 CPU sec, 2.478 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 288674, 15, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 417, 818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2034) [M::mem_pestat] mean and std.dev: (459.17, 346.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 247, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (272.39, 136.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (91, 350, 619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1675) [M::mem_pestat] mean and std.dev: (342.38, 310.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2203) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 339, 902) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2378) [M::mem_pestat] mean and std.dev: (530.68, 526.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3116) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698006 sequences (100000125 bp) in 1.820 CPU sec, 1.824 real sec... [M::mem_process_seqs] Processed 698078 reads in 163.678 CPU sec, 5.429 real sec [M::mem_process_seqs] Processed 697846 reads in 102.133 CPU sec, 3.234 real sec sorting block 8, #reads = 1088004 mem = 536882064 peak = 877858816 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 288564, 26, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 201, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (213.12, 71.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 246, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (272.13, 136.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (268, 1021, 2076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5692) [M::mem_pestat] mean and std.dev: (624.25, 582.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7500) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 265, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1257) [M::mem_pestat] mean and std.dev: (263.02, 181.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1620) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697532 sequences (100000011 bp) in 2.310 CPU sec, 2.410 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 289023, 20, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 265, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1150) [M::mem_pestat] mean and std.dev: (290.93, 191.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 246, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (271.55, 136.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (383, 1335, 1422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3500) [M::mem_pestat] mean and std.dev: (1001.00, 707.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4539) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 352, 626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1474) [M::mem_pestat] mean and std.dev: (427.34, 325.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1898) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697144 sequences (100000104 bp) in 1.810 CPU sec, 1.806 real sec... [M::mem_process_seqs] Processed 697764 reads in 159.237 CPU sec, 5.068 real sec sorting block 9, #reads = 1087272 mem = 536878713 peak = 877858816 [M::mem_process_seqs] Processed 698006 reads in 102.918 CPU sec, 3.263 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 289155, 19, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 221, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (227.72, 91.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (271.79, 136.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (391, 1037, 3556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9886) [M::mem_pestat] mean and std.dev: (1665.68, 1623.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13051) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 247, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (244.55, 114.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 10, #reads = 1086719 mem = 536882232 peak = 877858816 [M::process] read 696874 sequences (100000098 bp) in 2.802 CPU sec, 2.976 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 289042, 10, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 211, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1126) [M::mem_pestat] mean and std.dev: (240.08, 151.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (271.57, 134.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (442, 442, 712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1252) [M::mem_pestat] mean and std.dev: (524.56, 176.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1522) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 457, 879) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2265) [M::mem_pestat] mean and std.dev: (492.21, 459.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2958) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696696 sequences (100000208 bp) in 1.670 CPU sec, 1.676 real sec... [M::mem_process_seqs] Processed 697532 reads in 163.193 CPU sec, 5.203 real sec [M::mem_process_seqs] Processed 697144 reads in 113.531 CPU sec, 3.620 real sec sorting block 11, #reads = 1087322 mem = 536878437 peak = 877858816 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 288648, 14, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 257, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987) [M::mem_pestat] mean and std.dev: (293.71, 176.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1261) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (271.54, 134.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (135, 562, 929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2517) [M::mem_pestat] mean and std.dev: (489.77, 333.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3311) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 267, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1124) [M::mem_pestat] mean and std.dev: (310.27, 182.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1441) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696400 sequences (100000108 bp) in 2.738 CPU sec, 2.884 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 288195, 16, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 286, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1098) [M::mem_pestat] mean and std.dev: (344.87, 213.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1398) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (270.78, 133.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (761, 1518, 4618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12332) [M::mem_pestat] mean and std.dev: (2333.25, 2525.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16189) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 232, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1195) [M::mem_pestat] mean and std.dev: (298.19, 227.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1534) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696874 reads in 162.264 CPU sec, 5.167 real sec [M::process] read 696822 sequences (100000074 bp) in 1.969 CPU sec, 1.995 real sec... [M::mem_process_seqs] Processed 696696 reads in 104.910 CPU sec, 3.312 real sec sorting block 12, #reads = 1086094 mem = 536881864 peak = 877858816 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 288307, 18, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 204, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (224.40, 120.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 247, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (269.52, 132.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (487, 1394, 1544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3658) [M::mem_pestat] mean and std.dev: (1089.19, 779.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4715) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 250, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052) [M::mem_pestat] mean and std.dev: (284.70, 157.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1334) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696984 sequences (100000144 bp) in 2.411 CPU sec, 2.563 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 288222, 31, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 331, 1477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4051) [M::mem_pestat] mean and std.dev: (651.94, 757.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5338) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 246, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (269.36, 132.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (375, 1305, 2060) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5430) [M::mem_pestat] mean and std.dev: (1326.10, 1059.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7115) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 287, 922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2308) [M::mem_pestat] mean and std.dev: (459.45, 386.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3001) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696400 reads in 158.392 CPU sec, 5.043 real sec [M::process] read 697064 sequences (100000117 bp) in 1.948 CPU sec, 1.964 real sec... [M::mem_process_seqs] Processed 696822 reads in 103.468 CPU sec, 3.264 real sec sorting block 13, #reads = 1085644 mem = 536887394 peak = 877858816 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 288539, 22, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 234, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (267.60, 148.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (268.26, 131.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (386, 765, 1818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4682) [M::mem_pestat] mean and std.dev: (763.83, 904.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6114) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 289, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (240.94, 85.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696994 sequences (100000152 bp) in 2.382 CPU sec, 2.492 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 288334, 22, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 225, 696) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1774) [M::mem_pestat] mean and std.dev: (376.30, 310.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2313) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (268.60, 131.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (368, 803, 3000) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8264) [M::mem_pestat] mean and std.dev: (1991.68, 2257.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11021) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 319, 719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1739) [M::mem_pestat] mean and std.dev: (398.82, 293.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2249) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696984 reads in 152.198 CPU sec, 4.850 real sec sorting block 14, #reads = 1085110 mem = 536883354 peak = 877858816 [M::mem_process_seqs] Processed 697064 reads in 97.873 CPU sec, 3.084 real sec [M::process] read 696944 sequences (100000079 bp) in 2.066 CPU sec, 2.115 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 288690, 11, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 283, 1066) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2890) [M::mem_pestat] mean and std.dev: (571.13, 689.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3802) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 245, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (268.21, 131.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (658, 987, 2040) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4804) [M::mem_pestat] mean and std.dev: (1012.40, 691.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6186) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 307, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (284.68, 112.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1155) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 15, #reads = 1085016 mem = 536879851 peak = 910938112 [M::process] read 696752 sequences (100000179 bp) in 2.389 CPU sec, 2.502 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 287818, 17, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 327, 755) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1869) [M::mem_pestat] mean and std.dev: (466.73, 385.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 246, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (268.61, 131.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (162, 447, 909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2403) [M::mem_pestat] mean and std.dev: (538.80, 550.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3150) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 243, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845) [M::mem_pestat] mean and std.dev: (286.30, 166.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696392 sequences (100000124 bp) in 1.430 CPU sec, 1.427 real sec... [M::mem_process_seqs] Processed 696994 reads in 158.655 CPU sec, 5.057 real sec [M::mem_process_seqs] Processed 696944 reads in 104.319 CPU sec, 3.317 real sec sorting block 16, #reads = 1085927 mem = 536880146 peak = 910938112 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 288418, 19, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 280, 588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1328) [M::mem_pestat] mean and std.dev: (347.07, 207.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 247, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (269.53, 132.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (435, 702, 1134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2532) [M::mem_pestat] mean and std.dev: (806.47, 597.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3231) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 271, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1058) [M::mem_pestat] mean and std.dev: (337.75, 250.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1355) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696486 sequences (100000200 bp) in 2.779 CPU sec, 2.937 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 287507, 27, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 243, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000) [M::mem_pestat] mean and std.dev: (258.31, 156.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1279) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (270.23, 132.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (533, 1450, 2320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5894) [M::mem_pestat] mean and std.dev: (1608.89, 1198.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7681) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 301, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1228) [M::mem_pestat] mean and std.dev: (322.08, 201.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1588) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696752 reads in 159.070 CPU sec, 5.067 real sec [M::process] read 696048 sequences (100000297 bp) in 1.595 CPU sec, 1.595 real sec... [M::mem_process_seqs] Processed 696392 reads in 103.881 CPU sec, 3.286 real sec sorting block 17, #reads = 1084643 mem = 536878417 peak = 910938112 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 288115, 25, 72) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 270, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1303) [M::mem_pestat] mean and std.dev: (291.92, 209.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (272.27, 134.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (414, 561, 1966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5070) [M::mem_pestat] mean and std.dev: (1156.20, 1239.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6622) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 299, 695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1669) [M::mem_pestat] mean and std.dev: (443.27, 388.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2156) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696068 sequences (100000281 bp) in 2.688 CPU sec, 2.800 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 287688, 13, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 276, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (260.44, 134.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 251, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (274.38, 135.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (538, 956, 2545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6559) [M::mem_pestat] mean and std.dev: (937.91, 738.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8566) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 386, 613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1365) [M::mem_pestat] mean and std.dev: (383.33, 198.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1741) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696486 reads in 157.145 CPU sec, 4.993 real sec [M::process] read 703596 sequences (100000069 bp) in 1.930 CPU sec, 1.938 real sec... sorting block 18, #reads = 1083186 mem = 536880041 peak = 910938112 [M::mem_process_seqs] Processed 696048 reads in 102.276 CPU sec, 3.227 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 287826, 18, 83) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 210, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (315.36, 218.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1190) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (274.05, 134.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (538, 699, 1399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3121) [M::mem_pestat] mean and std.dev: (804.38, 444.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3982) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 322, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1097) [M::mem_pestat] mean and std.dev: (326.79, 199.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1395) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 19, #reads = 1082305 mem = 536880758 peak = 910938112 [M::process] read 703142 sequences (100000211 bp) in 2.512 CPU sec, 2.628 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 290782, 21, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 258, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1303) [M::mem_pestat] mean and std.dev: (314.29, 234.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 230, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (254.79, 127.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (259, 431, 1014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2524) [M::mem_pestat] mean and std.dev: (595.84, 543.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3279) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 332, 672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1586) [M::mem_pestat] mean and std.dev: (410.69, 329.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2043) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702456 sequences (100000041 bp) in 1.810 CPU sec, 1.816 real sec... [M::mem_process_seqs] Processed 696068 reads in 167.692 CPU sec, 5.567 real sec [M::mem_process_seqs] Processed 703596 reads in 105.660 CPU sec, 3.340 real sec sorting block 20, #reads = 1085564 mem = 536880558 peak = 910938112 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 290558, 16, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 300, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018) [M::mem_pestat] mean and std.dev: (305.41, 162.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1300) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 232, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (257.64, 130.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (326, 1242, 5123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14717) [M::mem_pestat] mean and std.dev: (2402.50, 2463.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19514) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 246, 791) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2023) [M::mem_pestat] mean and std.dev: (434.02, 436.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2639) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701802 sequences (100000211 bp) in 2.462 CPU sec, 2.604 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 290400, 18, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 324, 899) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2363) [M::mem_pestat] mean and std.dev: (428.33, 444.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3095) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 234, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (259.29, 130.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (478, 2281, 2371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6157) [M::mem_pestat] mean and std.dev: (1442.61, 945.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8050) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 522, 788) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1926) [M::mem_pestat] mean and std.dev: (527.18, 403.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2495) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 703142 reads in 157.655 CPU sec, 5.016 real sec [M::process] read 701622 sequences (100000007 bp) in 1.862 CPU sec, 1.879 real sec... [M::mem_process_seqs] Processed 702456 reads in 103.276 CPU sec, 3.262 real sec sorting block 21, #reads = 1091573 mem = 536880993 peak = 910938112 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 290030, 21, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 272, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1162) [M::mem_pestat] mean and std.dev: (312.62, 206.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 236, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (260.99, 131.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (546, 974, 7746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 22146) [M::mem_pestat] mean and std.dev: (3202.24, 3286.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 29346) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 250, 700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1804) [M::mem_pestat] mean and std.dev: (380.10, 303.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2356) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701284 sequences (100000113 bp) in 2.530 CPU sec, 2.660 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 290205, 25, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 205, 569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1433) [M::mem_pestat] mean and std.dev: (266.17, 200.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1865) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 237, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (262.62, 133.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (594, 826, 4507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12333) [M::mem_pestat] mean and std.dev: (2031.28, 1994.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16246) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 266, 578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1274) [M::mem_pestat] mean and std.dev: (377.61, 267.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1622) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701802 reads in 155.187 CPU sec, 4.936 real sec [M::process] read 700830 sequences (100000276 bp) in 2.038 CPU sec, 2.054 real sec... [M::mem_process_seqs] Processed 701622 reads in 100.791 CPU sec, 3.174 real sec sorting block 22, #reads = 1090510 mem = 536878895 peak = 910938112 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 290026, 18, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 277, 604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1480) [M::mem_pestat] mean and std.dev: (296.87, 201.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1918) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 237, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (263.04, 132.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (187, 730, 1088) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2890) [M::mem_pestat] mean and std.dev: (762.88, 764.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3821) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 310, 584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1278) [M::mem_pestat] mean and std.dev: (383.40, 246.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1625) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700468 sequences (100000181 bp) in 2.529 CPU sec, 2.665 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 289574, 17, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 380, 683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1695) [M::mem_pestat] mean and std.dev: (410.98, 313.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2201) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 239, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (265.19, 133.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (335, 392, 738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1544) [M::mem_pestat] mean and std.dev: (425.93, 300.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1947) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 339, 1140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2984) [M::mem_pestat] mean and std.dev: (640.40, 629.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3906) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701284 reads in 152.624 CPU sec, 4.861 real sec sorting block 23, #reads = 1091002 mem = 536877573 peak = 910938112 [M::mem_process_seqs] Processed 700830 reads in 101.312 CPU sec, 3.187 real sec [M::process] read 699910 sequences (100000012 bp) in 2.191 CPU sec, 2.241 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 290243, 25, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 282, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (241.11, 136.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 240, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (265.98, 134.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (77, 552, 1752) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5102) [M::mem_pestat] mean and std.dev: (601.55, 762.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6777) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 337, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (326.25, 153.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1063) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 24, #reads = 1089674 mem = 536884986 peak = 910938112 [M::process] read 699422 sequences (100000213 bp) in 2.504 CPU sec, 2.649 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 289329, 30, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 254, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961) [M::mem_pestat] mean and std.dev: (276.92, 139.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1230) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 242, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (268.31, 135.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (171, 1112, 2321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6621) [M::mem_pestat] mean and std.dev: (1431.62, 1341.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8771) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (283, 324, 709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1561) [M::mem_pestat] mean and std.dev: (489.03, 404.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2106) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700468 reads in 153.428 CPU sec, 4.890 real sec [M::mem_process_seqs] Processed 699910 reads in 99.986 CPU sec, 3.151 real sec [M::process] read 699404 sequences (100000253 bp) in 2.094 CPU sec, 2.155 real sec... sorting block 25, #reads = 1090433 mem = 536876846 peak = 910938112 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 289256, 19, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 334, 718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1862) [M::mem_pestat] mean and std.dev: (409.02, 373.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 244, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (269.11, 135.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (731, 1293, 2734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6740) [M::mem_pestat] mean and std.dev: (1500.59, 1162.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8743) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 323, 689) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1653) [M::mem_pestat] mean and std.dev: (381.83, 252.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2135) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698778 sequences (100000055 bp) in 2.594 CPU sec, 2.713 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 289819, 15, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 361, 681) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1655) [M::mem_pestat] mean and std.dev: (401.54, 295.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2142) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 243, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (269.13, 136.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (379, 781, 1998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5236) [M::mem_pestat] mean and std.dev: (1093.53, 873.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6855) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 239, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921) [M::mem_pestat] mean and std.dev: (268.33, 126.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699422 reads in 151.694 CPU sec, 4.832 real sec [M::mem_process_seqs] Processed 699404 reads in 98.816 CPU sec, 3.115 real sec [M::process] read 698540 sequences (100000121 bp) in 2.150 CPU sec, 2.212 real sec... sorting block 26, #reads = 1088557 mem = 536878333 peak = 910938112 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 289416, 18, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 238, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837) [M::mem_pestat] mean and std.dev: (272.15, 140.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 245, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (269.36, 134.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (194, 565, 2428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6896) [M::mem_pestat] mean and std.dev: (1079.41, 1352.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9130) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 246, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (241.85, 102.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698608 sequences (100000000 bp) in 2.234 CPU sec, 2.353 real sec... [M::process] read 698172 sequences (100000192 bp) in 1.390 CPU sec, 1.389 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 288754, 26, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 428, 714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1794) [M::mem_pestat] mean and std.dev: (434.84, 345.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2334) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 246, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (271.12, 136.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (216, 627, 1822) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5034) [M::mem_pestat] mean and std.dev: (750.23, 840.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6640) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (248, 318, 639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1421) [M::mem_pestat] mean and std.dev: (352.68, 186.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1812) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698778 reads in 157.354 CPU sec, 5.015 real sec sorting block 27, #reads = 1089067 mem = 536880907 peak = 910938112 [M::mem_process_seqs] Processed 698540 reads in 101.422 CPU sec, 3.219 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 288426, 13, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 324, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052) [M::mem_pestat] mean and std.dev: (345.59, 210.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1342) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 246, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (272.16, 137.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (378, 915, 2405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6459) [M::mem_pestat] mean and std.dev: (1167.00, 1214.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8486) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 345, 694) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1672) [M::mem_pestat] mean and std.dev: (530.64, 415.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2193) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 28, #reads = 1088219 mem = 536880662 peak = 910938112 [M::process] read 697746 sequences (100000206 bp) in 2.500 CPU sec, 2.651 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 288487, 17, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 394, 1534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4222) [M::mem_pestat] mean and std.dev: (820.09, 909.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 247, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (272.50, 137.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (198, 531, 1349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3651) [M::mem_pestat] mean and std.dev: (684.75, 568.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4802) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (248, 331, 793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1883) [M::mem_pestat] mean and std.dev: (498.17, 386.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2428) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697362 sequences (100000210 bp) in 1.850 CPU sec, 1.851 real sec... [M::mem_process_seqs] Processed 698608 reads in 161.377 CPU sec, 5.341 real sec [M::mem_process_seqs] Processed 698172 reads in 101.975 CPU sec, 3.213 real sec sorting block 29, #reads = 1087790 mem = 536880308 peak = 910938112 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 289316, 9, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 202, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988) [M::mem_pestat] mean and std.dev: (267.45, 185.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1272) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 248, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (273.36, 137.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 292, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937) [M::mem_pestat] mean and std.dev: (301.70, 184.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1196) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 696752 sequences (100000228 bp) in 2.579 CPU sec, 2.710 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 288885, 19, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 446, 782) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1844) [M::mem_pestat] mean and std.dev: (510.07, 346.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 249, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (273.65, 136.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (54, 79, 710) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2022) [M::mem_pestat] mean and std.dev: (373.05, 488.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2678) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 332, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037) [M::mem_pestat] mean and std.dev: (301.98, 145.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1324) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697746 reads in 154.411 CPU sec, 4.894 real sec [M::process] read 697394 sequences (100000117 bp) in 1.960 CPU sec, 1.968 real sec... [M::mem_process_seqs] Processed 697362 reads in 101.523 CPU sec, 3.199 real sec sorting block 30, #reads = 1088270 mem = 536879565 peak = 911011840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 289064, 17, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 249, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (214.94, 83.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 251, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (276.12, 138.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1061, 1545, 3612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8714) [M::mem_pestat] mean and std.dev: (2289.35, 1752.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11265) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 299, 1092) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2884) [M::mem_pestat] mean and std.dev: (560.12, 572.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3780) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696816 sequences (100000257 bp) in 3.039 CPU sec, 3.198 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 288814, 29, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 327, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066) [M::mem_pestat] mean and std.dev: (323.78, 199.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1373) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (271.07, 134.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (408, 925, 1483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3633) [M::mem_pestat] mean and std.dev: (735.08, 502.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4708) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 282, 696) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1752) [M::mem_pestat] mean and std.dev: (313.98, 253.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2280) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696752 reads in 155.467 CPU sec, 4.943 real sec [M::mem_process_seqs] Processed 697394 reads in 103.119 CPU sec, 3.246 real sec sorting block 31, #reads = 1088088 mem = 536880723 peak = 911011840 [M::process] read 696670 sequences (100000108 bp) in 2.248 CPU sec, 2.331 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 288076, 26, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (222, 316, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (285.54, 122.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1142) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (270.76, 133.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (369, 934, 2736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7470) [M::mem_pestat] mean and std.dev: (1377.12, 1206.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9837) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 218, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (250.90, 120.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1083) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 32, #reads = 1086302 mem = 536877506 peak = 911011840 [M::process] read 696626 sequences (100000207 bp) in 2.503 CPU sec, 2.654 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 288356, 19, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 308, 671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1659) [M::mem_pestat] mean and std.dev: (337.92, 311.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2153) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (271.85, 133.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (553, 797, 1998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4888) [M::mem_pestat] mean and std.dev: (1056.94, 902.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6333) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 289, 622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1486) [M::mem_pestat] mean and std.dev: (326.11, 201.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1918) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696816 reads in 159.186 CPU sec, 5.071 real sec [M::mem_process_seqs] Processed 696670 reads in 103.721 CPU sec, 3.265 real sec [M::process] read 696718 sequences (100000069 bp) in 2.215 CPU sec, 2.286 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 288383, 37, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 338, 711) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1663) [M::mem_pestat] mean and std.dev: (425.43, 300.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2139) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 248, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (271.13, 133.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (355, 592, 1411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3523) [M::mem_pestat] mean and std.dev: (918.84, 756.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4579) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 314, 625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1483) [M::mem_pestat] mean and std.dev: (380.74, 280.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1912) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 33, #reads = 1085939 mem = 536875919 peak = 911011840 [M::process] read 696946 sequences (100000073 bp) in 2.699 CPU sec, 2.854 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 288043, 19, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 257, 863) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2233) [M::mem_pestat] mean and std.dev: (451.04, 478.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2918) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 247, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (269.40, 131.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (120, 792, 1553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4419) [M::mem_pestat] mean and std.dev: (910.33, 937.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5852) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 300, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981) [M::mem_pestat] mean and std.dev: (332.99, 183.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1227) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696626 reads in 157.559 CPU sec, 5.011 real sec [M::mem_process_seqs] Processed 696718 reads in 105.156 CPU sec, 3.311 real sec [M::process] read 696526 sequences (100000015 bp) in 2.120 CPU sec, 2.186 real sec... sorting block 34, #reads = 1086305 mem = 536880726 peak = 911011840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 288405, 16, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 382, 1075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2811) [M::mem_pestat] mean and std.dev: (564.23, 507.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 247, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (270.03, 132.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (231, 411, 1976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5466) [M::mem_pestat] mean and std.dev: (1205.19, 1570.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7487) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 360, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (326.25, 144.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696866 sequences (100000084 bp) in 2.600 CPU sec, 2.723 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 288555, 27, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 233, 797) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2117) [M::mem_pestat] mean and std.dev: (414.57, 387.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (270.85, 133.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (836, 1003, 6941) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 19151) [M::mem_pestat] mean and std.dev: (2688.67, 2939.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 25256) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 308, 554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1262) [M::mem_pestat] mean and std.dev: (340.98, 170.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1616) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696946 reads in 159.606 CPU sec, 5.071 real sec [M::process] read 697026 sequences (100000149 bp) in 1.956 CPU sec, 1.983 real sec... [M::mem_process_seqs] Processed 696526 reads in 104.794 CPU sec, 3.495 real sec sorting block 35, #reads = 1085872 mem = 536878766 peak = 917667840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 288150, 15, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 275, 685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1695) [M::mem_pestat] mean and std.dev: (386.17, 294.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2200) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (271.24, 134.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (234, 449, 855) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2097) [M::mem_pestat] mean and std.dev: (477.38, 517.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2718) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (335, 447, 1136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2738) [M::mem_pestat] mean and std.dev: (669.37, 528.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3539) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696914 sequences (100000091 bp) in 2.595 CPU sec, 2.751 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 288492, 21, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 229, 680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1696) [M::mem_pestat] mean and std.dev: (313.59, 280.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2204) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 247, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (269.87, 132.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (158, 455, 1335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3689) [M::mem_pestat] mean and std.dev: (666.95, 773.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4866) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 280, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1212) [M::mem_pestat] mean and std.dev: (302.84, 207.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1559) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696866 reads in 161.295 CPU sec, 5.317 real sec [M::process] read 696900 sequences (100000091 bp) in 1.924 CPU sec, 1.929 real sec... sorting block 36, #reads = 1085527 mem = 536877998 peak = 917667840 [M::mem_process_seqs] Processed 697026 reads in 109.707 CPU sec, 3.476 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 288161, 14, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 274, 578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1404) [M::mem_pestat] mean and std.dev: (361.00, 289.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1817) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 247, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (270.46, 133.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (313, 523, 2637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7285) [M::mem_pestat] mean and std.dev: (1429.07, 1773.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9609) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 274, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040) [M::mem_pestat] mean and std.dev: (303.62, 200.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1326) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 37, #reads = 1084797 mem = 536881318 peak = 1015554048 [M::process] read 697134 sequences (100000196 bp) in 2.403 CPU sec, 2.536 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 288232, 20, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 260, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1237) [M::mem_pestat] mean and std.dev: (347.97, 269.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 247, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (270.42, 133.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (369, 945, 1353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3321) [M::mem_pestat] mean and std.dev: (985.53, 771.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4305) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 302, 596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1368) [M::mem_pestat] mean and std.dev: (299.12, 144.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1754) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696638 sequences (100000042 bp) in 1.470 CPU sec, 1.479 real sec... [M::mem_process_seqs] Processed 696914 reads in 161.402 CPU sec, 5.142 real sec [M::mem_process_seqs] Processed 696900 reads in 105.897 CPU sec, 3.354 real sec sorting block 38, #reads = 1084188 mem = 536879138 peak = 1015554048 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 288430, 24, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 281, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1176) [M::mem_pestat] mean and std.dev: (346.67, 236.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (271.27, 134.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (183, 878, 2483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7083) [M::mem_pestat] mean and std.dev: (1420.17, 1742.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9383) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 258, 547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1199) [M::mem_pestat] mean and std.dev: (276.85, 141.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1525) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696660 sequences (100000275 bp) in 2.722 CPU sec, 2.851 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 288089, 15, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 268, 672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1602) [M::mem_pestat] mean and std.dev: (298.26, 178.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2067) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (272.76, 135.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (605, 1367, 3846) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10328) [M::mem_pestat] mean and std.dev: (2095.60, 2212.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13569) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 296, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007) [M::mem_pestat] mean and std.dev: (281.41, 191.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1290) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702832 sequences (100000123 bp) in 1.646 CPU sec, 1.649 real sec... [M::mem_process_seqs] Processed 697134 reads in 161.748 CPU sec, 5.133 real sec [M::mem_process_seqs] Processed 696638 reads in 108.377 CPU sec, 3.426 real sec sorting block 39, #reads = 1084999 mem = 536878925 peak = 1015554048 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 288637, 31, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 255, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (281.46, 160.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (273.54, 135.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (143, 267, 1166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3212) [M::mem_pestat] mean and std.dev: (636.83, 693.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4235) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 346, 911) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2411) [M::mem_pestat] mean and std.dev: (464.04, 382.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3161) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704370 sequences (100000079 bp) in 2.885 CPU sec, 3.050 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 290227, 18, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 339, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093) [M::mem_pestat] mean and std.dev: (321.19, 217.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 232, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (257.25, 129.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (305, 874, 2046) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5528) [M::mem_pestat] mean and std.dev: (1231.89, 1259.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7269) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (253, 359, 539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1111) [M::mem_pestat] mean and std.dev: (341.45, 166.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1397) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704186 sequences (100000172 bp) in 1.582 CPU sec, 1.584 real sec... [M::mem_process_seqs] Processed 696660 reads in 168.374 CPU sec, 5.369 real sec sorting block 40, #reads = 1082561 mem = 536879883 peak = 1015554048 [M::mem_process_seqs] Processed 702832 reads in 111.771 CPU sec, 3.540 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 290834, 27, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 288, 1048) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2878) [M::mem_pestat] mean and std.dev: (518.53, 534.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 228, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (254.79, 129.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (175, 684, 1036) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2758) [M::mem_pestat] mean and std.dev: (572.04, 422.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3619) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 293, 639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1579) [M::mem_pestat] mean and std.dev: (331.80, 255.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2049) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 41, #reads = 1086094 mem = 536880388 peak = 1015554048 [M::process] read 703538 sequences (100000282 bp) in 2.561 CPU sec, 2.699 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 290882, 16, 73) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 263, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1248) [M::mem_pestat] mean and std.dev: (296.97, 217.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 229, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (254.66, 128.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (315, 2088, 4448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12714) [M::mem_pestat] mean and std.dev: (2032.94, 1829.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16847) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 327, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937) [M::mem_pestat] mean and std.dev: (346.44, 193.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1178) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703298 sequences (100000296 bp) in 1.825 CPU sec, 1.828 real sec... [M::mem_process_seqs] Processed 704370 reads in 166.629 CPU sec, 5.582 real sec [M::mem_process_seqs] Processed 704186 reads in 106.077 CPU sec, 3.352 real sec sorting block 42, #reads = 1090639 mem = 536881191 peak = 1015554048 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 291011, 12, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 250, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787) [M::mem_pestat] mean and std.dev: (252.03, 132.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 995) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 231, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (256.36, 129.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (724, 2711, 6676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18580) [M::mem_pestat] mean and std.dev: (2859.50, 2445.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 24532) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 220, 675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1733) [M::mem_pestat] mean and std.dev: (342.38, 304.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2262) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702710 sequences (100000184 bp) in 2.790 CPU sec, 2.944 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 290596, 21, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 290, 1049) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2881) [M::mem_pestat] mean and std.dev: (550.96, 615.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3797) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 231, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (257.62, 130.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (953, 1481, 1748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3338) [M::mem_pestat] mean and std.dev: (1354.00, 723.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4248) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 237, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (274.09, 138.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 703538 reads in 157.223 CPU sec, 5.010 real sec [M::process] read 702508 sequences (100000258 bp) in 1.710 CPU sec, 1.721 real sec... [M::mem_process_seqs] Processed 703298 reads in 104.071 CPU sec, 3.300 real sec sorting block 43, #reads = 1091989 mem = 536879054 peak = 1015554048 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 290857, 15, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 300, 725) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1807) [M::mem_pestat] mean and std.dev: (387.10, 300.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2348) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 232, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (259.05, 131.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (330, 435, 1030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2430) [M::mem_pestat] mean and std.dev: (616.73, 482.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3130) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 391, 929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2377) [M::mem_pestat] mean and std.dev: (573.56, 505.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3101) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701334 sequences (100000063 bp) in 2.401 CPU sec, 2.542 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 290443, 26, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 367, 602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1514) [M::mem_pestat] mean and std.dev: (446.46, 350.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1970) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 232, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (258.58, 131.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (460, 906, 1360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3160) [M::mem_pestat] mean and std.dev: (906.62, 645.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4060) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 401, 1011) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2677) [M::mem_pestat] mean and std.dev: (580.87, 645.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3510) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702710 reads in 151.982 CPU sec, 4.830 real sec [M::process] read 701168 sequences (100000123 bp) in 2.039 CPU sec, 2.061 real sec... [M::mem_process_seqs] Processed 702508 reads in 100.675 CPU sec, 3.168 real sec sorting block 44, #reads = 1090829 mem = 536879048 peak = 1015554048 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 290205, 13, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 232, 785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2049) [M::mem_pestat] mean and std.dev: (425.33, 382.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 235, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (262.75, 133.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (188, 402, 1775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4949) [M::mem_pestat] mean and std.dev: (768.33, 1033.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6536) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 283, 570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1362) [M::mem_pestat] mean and std.dev: (294.51, 164.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1758) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700708 sequences (100000044 bp) in 2.846 CPU sec, 3.006 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 290442, 12, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 228, 663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1637) [M::mem_pestat] mean and std.dev: (352.10, 297.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2124) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 237, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (263.86, 134.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (157, 244, 2209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6313) [M::mem_pestat] mean and std.dev: (845.92, 1065.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8365) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 255, 812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2048) [M::mem_pestat] mean and std.dev: (375.96, 313.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2666) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701334 reads in 155.848 CPU sec, 4.951 real sec sorting block 45, #reads = 1089445 mem = 536877454 peak = 1015554048 [M::mem_process_seqs] Processed 701168 reads in 104.188 CPU sec, 3.282 real sec [M::process] read 700716 sequences (100000113 bp) in 2.099 CPU sec, 2.174 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 289807, 23, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (239, 293, 551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1175) [M::mem_pestat] mean and std.dev: (380.62, 227.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1487) [M::mem_pestat] analyzing insert size distribution for orientation FR... sorting block 46, #reads = 1089349 mem = 536879118 peak = 1015554048 [M::mem_pestat] (25, 50, 75) percentile: (158, 238, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (264.82, 134.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1040, 2036, 2860) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6500) [M::mem_pestat] mean and std.dev: (1807.48, 1399.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8320) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 273, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052) [M::mem_pestat] mean and std.dev: (317.90, 192.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1328) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700206 sequences (100000125 bp) in 2.667 CPU sec, 2.795 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 289800, 18, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (302, 488, 1010) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2426) [M::mem_pestat] mean and std.dev: (519.84, 403.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3134) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 238, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (264.54, 133.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (270, 967, 1447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3801) [M::mem_pestat] mean and std.dev: (842.00, 878.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4978) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 285, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (294.62, 161.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1080) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699580 sequences (100000113 bp) in 1.440 CPU sec, 1.440 real sec... [M::mem_process_seqs] Processed 700708 reads in 156.009 CPU sec, 4.958 real sec [M::mem_process_seqs] Processed 700716 reads in 103.170 CPU sec, 3.259 real sec sorting block 47, #reads = 1088187 mem = 536881563 peak = 1015554048 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 289865, 12, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 240, 730) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1892) [M::mem_pestat] mean and std.dev: (385.60, 368.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 240, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (266.59, 135.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (205, 1356, 2967) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8491) [M::mem_pestat] mean and std.dev: (1581.50, 1515.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11253) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 336, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (299.46, 120.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699710 sequences (100000021 bp) in 2.951 CPU sec, 3.109 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 289109, 24, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 362, 607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1453) [M::mem_pestat] mean and std.dev: (336.38, 184.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1876) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 243, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (268.32, 135.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (299, 639, 1976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5330) [M::mem_pestat] mean and std.dev: (1099.71, 1019.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7007) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (263, 313, 1094) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2756) [M::mem_pestat] mean and std.dev: (574.74, 489.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3587) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700206 reads in 152.794 CPU sec, 4.859 real sec [M::mem_process_seqs] Processed 699580 reads in 101.195 CPU sec, 3.189 real sec [M::process] read 699390 sequences (100000230 bp) in 2.206 CPU sec, 2.290 real sec... sorting block 48, #reads = 1088921 mem = 536880286 peak = 1015554048 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 289589, 15, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 298, 698) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1772) [M::mem_pestat] mean and std.dev: (449.15, 348.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2309) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 243, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (269.41, 136.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (216, 370, 1204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3180) [M::mem_pestat] mean and std.dev: (787.20, 790.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4168) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 296, 880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2294) [M::mem_pestat] mean and std.dev: (397.00, 418.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3001) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699056 sequences (100000174 bp) in 2.432 CPU sec, 2.527 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 289871, 28, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 239, 582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1448) [M::mem_pestat] mean and std.dev: (358.68, 266.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1881) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 243, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (270.27, 137.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (389, 1169, 1280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3062) [M::mem_pestat] mean and std.dev: (854.83, 676.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3953) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 276, 588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1352) [M::mem_pestat] mean and std.dev: (384.72, 279.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1734) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699710 reads in 155.824 CPU sec, 4.958 real sec [M::process] read 698688 sequences (100000260 bp) in 1.997 CPU sec, 2.020 real sec... sorting block 49, #reads = 1089178 mem = 536880014 peak = 1015554048 [M::mem_process_seqs] Processed 699390 reads in 102.693 CPU sec, 3.229 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 289560, 23, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 239, 791) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2095) [M::mem_pestat] mean and std.dev: (414.89, 390.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 243, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (269.48, 136.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (744, 1667, 2205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5127) [M::mem_pestat] mean and std.dev: (1615.55, 1171.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6588) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 235, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974) [M::mem_pestat] mean and std.dev: (291.26, 166.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1236) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 50, #reads = 1089052 mem = 536879288 peak = 1015554048 [M::process] read 698768 sequences (100000262 bp) in 2.802 CPU sec, 2.958 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 288857, 11, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 335, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1225) [M::mem_pestat] mean and std.dev: (343.34, 230.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 245, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (270.87, 137.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (505, 527, 823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1459) [M::mem_pestat] mean and std.dev: (607.50, 331.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1934) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 297, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076) [M::mem_pestat] mean and std.dev: (319.80, 206.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1378) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699056 reads in 157.174 CPU sec, 5.237 real sec [M::mem_process_seqs] Processed 698688 reads in 104.283 CPU sec, 3.375 real sec [M::process] read 699106 sequences (100000070 bp) in 2.097 CPU sec, 2.152 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 288781, 18, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 256, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1263) [M::mem_pestat] mean and std.dev: (273.50, 187.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 246, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (271.47, 137.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (397, 1261, 1497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3697) [M::mem_pestat] mean and std.dev: (855.31, 755.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4797) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 324, 556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1204) [M::mem_pestat] mean and std.dev: (346.90, 173.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1528) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 51, #reads = 1088016 mem = 536878626 peak = 1015554048 [M::process] read 698926 sequences (100000120 bp) in 2.663 CPU sec, 2.860 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 289042, 5, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 237, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1305) [M::mem_pestat] mean and std.dev: (268.29, 205.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 241, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (265.20, 131.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 258, 601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1475) [M::mem_pestat] mean and std.dev: (295.21, 189.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1912) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698768 reads in 164.721 CPU sec, 5.382 real sec [M::mem_process_seqs] Processed 699106 reads in 111.245 CPU sec, 3.667 real sec [M::process] read 699248 sequences (100000207 bp) in 2.091 CPU sec, 2.175 real sec... sorting block 52, #reads = 1088134 mem = 536879854 peak = 1015554048 [M::process] read 697952 sequences (100000181 bp) in 2.685 CPU sec, 2.852 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 288990, 15, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 245, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1147) [M::mem_pestat] mean and std.dev: (307.00, 194.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 240, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (265.03, 132.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (397, 527, 1667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4207) [M::mem_pestat] mean and std.dev: (951.73, 788.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5477) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 263, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (250.08, 97.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699248 reads in 93.433 CPU sec, 3.108 real sec [M::process] read 696700 sequences (100000169 bp) in 2.090 CPU sec, 2.150 real sec... sorting block 53, #reads = 1087093 mem = 536878654 peak = 1015554048 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 289449, 18, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 269, 1459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4017) [M::mem_pestat] mean and std.dev: (777.90, 888.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5296) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 240, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (264.82, 131.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (137, 1213, 2401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6929) [M::mem_pestat] mean and std.dev: (1411.22, 1276.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9193) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 308, 985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2541) [M::mem_pestat] mean and std.dev: (532.86, 538.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3319) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 288888, 31, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 386, 684) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1664) [M::mem_pestat] mean and std.dev: (382.50, 263.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2154) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 246, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (270.56, 135.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (261, 669, 1213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3117) [M::mem_pestat] mean and std.dev: (761.41, 616.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4069) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (136, 241, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1081) [M::mem_pestat] mean and std.dev: (287.19, 216.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1396) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698926 reads in 256.141 CPU sec, 8.543 real sec [M::mem_process_seqs] Processed 697952 reads in 127.393 CPU sec, 4.435 real sec [M::process] read 697024 sequences (100000241 bp) in 2.544 CPU sec, 2.663 real sec... sorting block 54, #reads = 1087116 mem = 536880185 peak = 1015554048 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 288471, 30, 85) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 272, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034) [M::mem_pestat] mean and std.dev: (296.24, 184.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1328) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 250, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (273.72, 136.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (479, 1340, 2291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5915) [M::mem_pestat] mean and std.dev: (1756.60, 1464.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7727) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 314, 891) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2215) [M::mem_pestat] mean and std.dev: (568.66, 534.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2877) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696922 sequences (100000090 bp) in 2.391 CPU sec, 2.574 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 288024, 23, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 308, 811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2047) [M::mem_pestat] mean and std.dev: (445.93, 391.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (272.13, 135.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (435, 559, 588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (129, 894) [M::mem_pestat] mean and std.dev: (533.56, 63.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 333, 692) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1708) [M::mem_pestat] mean and std.dev: (476.69, 408.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2216) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696700 reads in 166.106 CPU sec, 5.439 real sec [M::process] read 697236 sequences (100000081 bp) in 1.918 CPU sec, 1.988 real sec... [M::mem_process_seqs] Processed 697024 reads in 112.445 CPU sec, 3.682 real sec sorting block 55, #reads = 1086816 mem = 536879296 peak = 1015554048 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 288285, 26, 76) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 319, 567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1297) [M::mem_pestat] mean and std.dev: (320.00, 168.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (272.49, 135.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (124, 546, 1767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5053) [M::mem_pestat] mean and std.dev: (934.12, 908.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6696) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 303, 1048) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2800) [M::mem_pestat] mean and std.dev: (521.24, 537.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3676) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696950 sequences (100000167 bp) in 2.704 CPU sec, 2.909 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 288451, 18, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 337, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1194) [M::mem_pestat] mean and std.dev: (357.34, 258.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (271.87, 135.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (389, 1150, 1857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4793) [M::mem_pestat] mean and std.dev: (901.75, 682.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6261) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 453, 693) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1625) [M::mem_pestat] mean and std.dev: (457.23, 322.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2091) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696922 reads in 164.646 CPU sec, 5.428 real sec sorting block 56, #reads = 1086737 mem = 536881161 peak = 1015554048 [M::mem_process_seqs] Processed 697236 reads in 110.323 CPU sec, 3.645 real sec [M::process] read 696958 sequences (100000180 bp) in 2.190 CPU sec, 2.241 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 288958, 16, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 367, 882) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2310) [M::mem_pestat] mean and std.dev: (404.61, 322.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3024) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 248, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (272.00, 135.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (500, 730, 2271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5813) [M::mem_pestat] mean and std.dev: (1110.47, 1040.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7584) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 277, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (273.97, 136.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 57, #reads = 1086240 mem = 536879442 peak = 1015554048 [M::process] read 697148 sequences (100000086 bp) in 2.710 CPU sec, 2.887 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 288714, 23, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 252, 600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1516) [M::mem_pestat] mean and std.dev: (277.16, 207.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1974) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (271.57, 134.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (235, 519, 1166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3028) [M::mem_pestat] mean and std.dev: (853.13, 735.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3959) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 328, 962) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2464) [M::mem_pestat] mean and std.dev: (575.98, 522.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3215) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696950 reads in 164.834 CPU sec, 5.641 real sec [M::mem_process_seqs] Processed 696958 reads in 109.925 CPU sec, 3.805 real sec [M::process] read 697524 sequences (100000000 bp) in 2.175 CPU sec, 2.231 real sec... sorting block 58, #reads = 1084806 mem = 536879019 peak = 1015554048 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 288111, 22, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 264, 598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1458) [M::mem_pestat] mean and std.dev: (350.18, 309.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1888) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 248, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (271.70, 135.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (346, 505, 2503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6817) [M::mem_pestat] mean and std.dev: (1109.45, 1058.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8974) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 288, 998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2636) [M::mem_pestat] mean and std.dev: (506.50, 530.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3455) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696166 sequences (100000216 bp) in 2.366 CPU sec, 2.523 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 288333, 10, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 264, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034) [M::mem_pestat] mean and std.dev: (315.14, 222.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1318) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (270.14, 134.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (79, 1488, 1954) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5704) [M::mem_pestat] mean and std.dev: (1393.80, 1536.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7579) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (143, 214, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1169) [M::mem_pestat] mean and std.dev: (285.91, 214.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1511) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697148 reads in 170.296 CPU sec, 5.644 real sec [M::process] read 697590 sequences (100000135 bp) in 2.105 CPU sec, 2.158 real sec... [M::mem_process_seqs] Processed 697524 reads in 116.210 CPU sec, 3.910 real sec sorting block 59, #reads = 1085580 mem = 536878987 peak = 1015554048 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 287853, 19, 77) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 350, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1064) [M::mem_pestat] mean and std.dev: (354.14, 234.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1361) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 251, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (275.56, 137.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (511, 753, 1174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2500) [M::mem_pestat] mean and std.dev: (814.72, 372.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3163) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 212, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (246.04, 141.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1089) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703874 sequences (100000042 bp) in 2.495 CPU sec, 2.742 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 288456, 18, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 223, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1185) [M::mem_pestat] mean and std.dev: (273.53, 179.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (271.99, 136.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (87, 627, 1163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3315) [M::mem_pestat] mean and std.dev: (625.06, 610.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4391) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 452, 921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2411) [M::mem_pestat] mean and std.dev: (523.31, 430.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3156) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696166 reads in 169.246 CPU sec, 5.711 real sec [M::process] read 703988 sequences (100000048 bp) in 2.059 CPU sec, 2.122 real sec... [M::mem_process_seqs] Processed 697590 reads in 121.089 CPU sec, 4.299 real sec sorting block 60, #reads = 1083450 mem = 536882544 peak = 1015554048 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 290879, 20, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 260, 1170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3192) [M::mem_pestat] mean and std.dev: (570.82, 584.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4203) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 229, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (255.44, 129.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (195, 390, 716) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1758) [M::mem_pestat] mean and std.dev: (479.53, 411.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2279) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 307, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (285.97, 113.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 61, #reads = 1083478 mem = 536879648 peak = 1097494528 [M::process] read 703610 sequences (100000195 bp) in 2.875 CPU sec, 3.265 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 290442, 24, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 271, 593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1459) [M::mem_pestat] mean and std.dev: (271.14, 168.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1892) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 228, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (255.13, 129.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (612, 808, 2609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6603) [M::mem_pestat] mean and std.dev: (1261.43, 1000.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8600) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 257, 580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1372) [M::mem_pestat] mean and std.dev: (309.86, 183.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1768) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 703874 reads in 161.043 CPU sec, 5.387 real sec [M::mem_process_seqs] Processed 703988 reads in 105.819 CPU sec, 3.509 real sec [M::process] read 703080 sequences (100000248 bp) in 2.327 CPU sec, 2.427 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 290582, 13, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 238, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (244.85, 116.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 230, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (256.57, 129.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (241, 422, 2356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6586) [M::mem_pestat] mean and std.dev: (800.46, 891.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8701) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 280, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1271) [M::mem_pestat] mean and std.dev: (265.30, 156.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1638) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 62, #reads = 1090699 mem = 536878030 peak = 1097494528 [M::process] read 702878 sequences (100000191 bp) in 2.316 CPU sec, 2.459 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 290578, 23, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 178, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (218.48, 140.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1021) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 232, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (257.23, 129.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (724, 1115, 1175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2077) [M::mem_pestat] mean and std.dev: (833.25, 441.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2601) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 225, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (233.84, 101.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702294 sequences (100000006 bp) in 1.633 CPU sec, 1.670 real sec... [M::mem_process_seqs] Processed 703610 reads in 163.716 CPU sec, 5.704 real sec [M::mem_process_seqs] Processed 703080 reads in 106.464 CPU sec, 4.111 real sec sorting block 63, #reads = 1091683 mem = 536879210 peak = 1097494528 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 290953, 16, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 307, 556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1382) [M::mem_pestat] mean and std.dev: (308.10, 190.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1795) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 232, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (257.67, 129.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (645, 845, 2687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6771) [M::mem_pestat] mean and std.dev: (975.29, 772.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8813) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 330, 651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1535) [M::mem_pestat] mean and std.dev: (414.00, 296.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1977) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703366 sequences (100000207 bp) in 2.775 CPU sec, 3.066 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 290492, 16, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 312, 652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1558) [M::mem_pestat] mean and std.dev: (323.44, 225.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2011) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 233, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (259.06, 130.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (611, 1471, 3198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8372) [M::mem_pestat] mean and std.dev: (1799.31, 1491.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10959) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 375, 952) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2412) [M::mem_pestat] mean and std.dev: (512.08, 432.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3142) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702878 reads in 159.400 CPU sec, 5.207 real sec [M::mem_process_seqs] Processed 702294 reads in 106.407 CPU sec, 3.469 real sec sorting block 64, #reads = 1092151 mem = 536882660 peak = 1097494528 [M::process] read 701920 sequences (100000083 bp) in 2.527 CPU sec, 2.684 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 290921, 16, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 337, 816) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2018) [M::mem_pestat] mean and std.dev: (458.51, 366.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 230, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (256.60, 130.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (423, 441, 1480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3594) [M::mem_pestat] mean and std.dev: (918.25, 763.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4651) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 392, 684) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1644) [M::mem_pestat] mean and std.dev: (443.66, 294.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2124) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 65, #reads = 1091786 mem = 536878125 peak = 1097494528 [M::process] read 702050 sequences (100000199 bp) in 2.340 CPU sec, 2.609 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 290285, 19, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 196, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1290) [M::mem_pestat] mean and std.dev: (319.48, 275.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 235, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (261.41, 132.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (562, 1565, 3533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9475) [M::mem_pestat] mean and std.dev: (2138.78, 2201.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12446) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 222, 685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1761) [M::mem_pestat] mean and std.dev: (371.11, 339.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2299) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 703366 reads in 162.185 CPU sec, 5.332 real sec [M::mem_process_seqs] Processed 701920 reads in 107.101 CPU sec, 3.572 real sec [M::process] read 701598 sequences (100000209 bp) in 2.132 CPU sec, 2.229 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 290108, 9, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 248, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (255.50, 139.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1016) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 235, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (261.28, 132.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 252, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986) [M::mem_pestat] mean and std.dev: (294.82, 162.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1252) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 66, #reads = 1090275 mem = 536879898 peak = 1097494528 [M::process] read 701652 sequences (100000002 bp) in 2.773 CPU sec, 2.932 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 290078, 19, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 395, 1525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4185) [M::mem_pestat] mean and std.dev: (695.86, 865.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 236, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (262.42, 132.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (64, 790, 3459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10249) [M::mem_pestat] mean and std.dev: (1878.53, 2116.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13644) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 331, 766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1928) [M::mem_pestat] mean and std.dev: (446.27, 373.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2509) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702050 reads in 161.057 CPU sec, 5.328 real sec [M::mem_process_seqs] Processed 701598 reads in 107.022 CPU sec, 3.591 real sec [M::process] read 700730 sequences (100000042 bp) in 2.282 CPU sec, 2.417 real sec... sorting block 67, #reads = 1090911 mem = 536882965 peak = 1097494528 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 290250, 17, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 274, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1121) [M::mem_pestat] mean and std.dev: (294.75, 189.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 236, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (262.00, 133.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (402, 730, 2786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7554) [M::mem_pestat] mean and std.dev: (1544.82, 1349.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9938) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 249, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (255.32, 131.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1171) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700370 sequences (100000156 bp) in 2.175 CPU sec, 2.372 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 290045, 9, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 237, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1126) [M::mem_pestat] mean and std.dev: (271.71, 182.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 238, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (264.88, 134.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 359, 590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1362) [M::mem_pestat] mean and std.dev: (421.77, 304.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1748) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701652 reads in 159.679 CPU sec, 5.211 real sec [M::process] read 699898 sequences (100000046 bp) in 1.879 CPU sec, 1.915 real sec... [M::mem_process_seqs] Processed 700730 reads in 106.286 CPU sec, 3.550 real sec sorting block 68, #reads = 1089728 mem = 536877939 peak = 1097494528 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 290025, 33, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 317, 821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2153) [M::mem_pestat] mean and std.dev: (480.73, 385.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2819) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 240, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (266.38, 135.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (509, 1070, 1235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2687) [M::mem_pestat] mean and std.dev: (696.69, 441.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3413) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 232, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951) [M::mem_pestat] mean and std.dev: (293.47, 174.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1207) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700144 sequences (100000105 bp) in 2.852 CPU sec, 3.027 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 289251, 28, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 349, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015) [M::mem_pestat] mean and std.dev: (273.00, 151.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1303) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 241, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (267.35, 135.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (527, 972, 1738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4160) [M::mem_pestat] mean and std.dev: (1191.75, 833.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5371) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 327, 1926) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5440) [M::mem_pestat] mean and std.dev: (1108.56, 1388.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7197) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700370 reads in 155.359 CPU sec, 5.113 real sec sorting block 69, #reads = 1090308 mem = 536882428 peak = 1097494528 [M::mem_process_seqs] Processed 699898 reads in 103.558 CPU sec, 3.281 real sec [M::process] read 699986 sequences (100000159 bp) in 2.254 CPU sec, 2.359 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 289688, 24, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 271, 807) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2017) [M::mem_pestat] mean and std.dev: (418.66, 353.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2622) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 240, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (267.40, 136.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (232, 987, 1778) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4870) [M::mem_pestat] mean and std.dev: (1364.12, 1344.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6741) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 270, 1071) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2841) [M::mem_pestat] mean and std.dev: (457.64, 452.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3726) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 70, #reads = 1090004 mem = 536878585 peak = 1097494528 [M::process] read 699286 sequences (100000222 bp) in 2.497 CPU sec, 2.628 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 289557, 34, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 224, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1247) [M::mem_pestat] mean and std.dev: (317.76, 202.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 241, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (268.33, 136.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (94, 485, 1160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3292) [M::mem_pestat] mean and std.dev: (780.67, 753.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4358) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 268, 707) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1751) [M::mem_pestat] mean and std.dev: (429.16, 397.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2273) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700144 reads in 159.856 CPU sec, 5.081 real sec [M::mem_process_seqs] Processed 699986 reads in 105.730 CPU sec, 3.329 real sec [M::process] read 698992 sequences (100000025 bp) in 2.141 CPU sec, 2.185 real sec... sorting block 71, #reads = 1088793 mem = 536885789 peak = 1097494528 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 289208, 14, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 260, 708) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1720) [M::mem_pestat] mean and std.dev: (446.70, 382.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2226) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 243, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (270.03, 137.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (184, 530, 1294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3514) [M::mem_pestat] mean and std.dev: (794.46, 841.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4624) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 341, 802) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2050) [M::mem_pestat] mean and std.dev: (398.46, 280.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2674) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698618 sequences (100000283 bp) in 2.875 CPU sec, 3.048 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 289363, 24, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 387, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027) [M::mem_pestat] mean and std.dev: (336.34, 181.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1294) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 245, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (270.97, 137.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (188, 1400, 3628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10508) [M::mem_pestat] mean and std.dev: (2471.79, 2803.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13948) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 238, 546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1310) [M::mem_pestat] mean and std.dev: (342.25, 224.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1692) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699286 reads in 159.780 CPU sec, 5.082 real sec [M::mem_process_seqs] Processed 698992 reads in 105.969 CPU sec, 3.340 real sec [M::process] read 698324 sequences (100000167 bp) in 2.018 CPU sec, 2.052 real sec... sorting block 72, #reads = 1087299 mem = 536881601 peak = 1097494528 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 289306, 36, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 342, 546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1232) [M::mem_pestat] mean and std.dev: (341.63, 193.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 245, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (270.75, 136.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (362, 975, 1599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4073) [M::mem_pestat] mean and std.dev: (966.12, 661.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5310) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 331, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1148) [M::mem_pestat] mean and std.dev: (300.29, 172.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1465) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698140 sequences (100000111 bp) in 2.849 CPU sec, 2.993 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 289102, 30, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 241, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (280.27, 145.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1081) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 246, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (271.24, 136.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (456, 590, 771) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1401) [M::mem_pestat] mean and std.dev: (611.79, 325.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1913) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 484, 1731) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4731) [M::mem_pestat] mean and std.dev: (772.43, 775.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6231) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697448 sequences (100000218 bp) in 1.499 CPU sec, 1.504 real sec... [M::mem_process_seqs] Processed 698618 reads in 166.085 CPU sec, 5.311 real sec sorting block 73, #reads = 1088704 mem = 536878106 peak = 1097494528 [M::mem_process_seqs] Processed 698324 reads in 110.631 CPU sec, 3.515 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 288749, 13, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 271, 542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1272) [M::mem_pestat] mean and std.dev: (312.37, 232.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 247, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (271.67, 136.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (381, 412, 2317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6189) [M::mem_pestat] mean and std.dev: (1298.54, 1496.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8125) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 283, 633) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1441) [M::mem_pestat] mean and std.dev: (331.93, 173.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1845) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 74, #reads = 1087520 mem = 536885118 peak = 1097494528 [M::process] read 697248 sequences (100000123 bp) in 2.586 CPU sec, 2.713 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 288838, 20, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 297, 547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1321) [M::mem_pestat] mean and std.dev: (347.84, 254.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1708) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 250, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (273.45, 136.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1196, 1920, 5630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14498) [M::mem_pestat] mean and std.dev: (2600.50, 2069.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18932) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 255, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (274.08, 128.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698140 reads in 166.985 CPU sec, 5.327 real sec [M::process] read 696772 sequences (100000124 bp) in 1.900 CPU sec, 1.909 real sec... [M::mem_process_seqs] Processed 697448 reads in 109.660 CPU sec, 3.522 real sec sorting block 75, #reads = 1086497 mem = 536876965 peak = 1097494528 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 288951, 27, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 279, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837) [M::mem_pestat] mean and std.dev: (273.02, 158.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 250, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (273.72, 136.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (153, 279, 1023) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2763) [M::mem_pestat] mean and std.dev: (519.25, 576.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3633) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (141, 220, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1311) [M::mem_pestat] mean and std.dev: (281.54, 262.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1701) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696352 sequences (100000152 bp) in 2.459 CPU sec, 2.583 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 288470, 21, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 237, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (257.43, 141.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 251, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (274.55, 136.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (163, 660, 1646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4612) [M::mem_pestat] mean and std.dev: (1076.24, 1185.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6095) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 288, 558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1294) [M::mem_pestat] mean and std.dev: (375.57, 275.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1662) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696252 sequences (100000204 bp) in 1.576 CPU sec, 1.581 real sec... [M::mem_process_seqs] Processed 697248 reads in 172.194 CPU sec, 5.554 real sec [M::mem_process_seqs] Processed 696772 reads in 110.677 CPU sec, 3.520 real sec sorting block 76, #reads = 1085903 mem = 536879480 peak = 1097494528 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 288526, 23, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 255, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (257.29, 141.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1175) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (274.89, 135.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (293, 963, 2262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6200) [M::mem_pestat] mean and std.dev: (1479.50, 1638.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8169) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 292, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074) [M::mem_pestat] mean and std.dev: (335.52, 180.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1354) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696342 sequences (100000090 bp) in 2.869 CPU sec, 3.010 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 287986, 13, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 335, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844) [M::mem_pestat] mean and std.dev: (296.85, 142.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 251, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (275.23, 136.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (329, 455, 1329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3329) [M::mem_pestat] mean and std.dev: (702.69, 531.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4329) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 296, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995) [M::mem_pestat] mean and std.dev: (315.75, 171.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1264) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696352 reads in 168.191 CPU sec, 5.353 real sec [M::process] read 696210 sequences (100000027 bp) in 1.963 CPU sec, 1.987 real sec... [M::mem_process_seqs] Processed 696252 reads in 111.544 CPU sec, 3.527 real sec sorting block 77, #reads = 1085195 mem = 536887676 peak = 1097494528 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 288390, 19, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 422, 884) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2180) [M::mem_pestat] mean and std.dev: (476.17, 395.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2828) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 251, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (274.50, 135.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (259, 1862, 3821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10945) [M::mem_pestat] mean and std.dev: (2496.21, 2499.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14507) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 286, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980) [M::mem_pestat] mean and std.dev: (301.00, 203.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1254) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696234 sequences (100000088 bp) in 2.466 CPU sec, 2.586 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 288042, 15, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (247, 466, 1998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5500) [M::mem_pestat] mean and std.dev: (964.77, 1108.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7251) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 251, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (273.61, 134.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (364, 1375, 4853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13831) [M::mem_pestat] mean and std.dev: (2282.73, 2109.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18320) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 244, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (227.77, 96.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695954 sequences (100000221 bp) in 1.856 CPU sec, 1.860 real sec... [M::mem_process_seqs] Processed 696342 reads in 171.872 CPU sec, 5.696 real sec sorting block 78, #reads = 1085178 mem = 536878195 peak = 1097494528 [M::mem_process_seqs] Processed 696210 reads in 107.947 CPU sec, 3.416 real sec sorting block 79, #reads = 1084931 mem = 536880527 peak = 1097494528 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 288359, 20, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 266, 894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2334) [M::mem_pestat] mean and std.dev: (472.67, 512.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3054) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 251, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (272.98, 133.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (288, 1035, 1934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5226) [M::mem_pestat] mean and std.dev: (1167.95, 1078.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6872) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 300, 565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1261) [M::mem_pestat] mean and std.dev: (363.58, 219.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1609) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695920 sequences (100000170 bp) in 2.334 CPU sec, 2.451 real sec... [M::process] read 695924 sequences (100000068 bp) in 1.457 CPU sec, 1.464 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 288535, 23, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 223, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (5, 500) [M::mem_pestat] mean and std.dev: (216.83, 57.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 251, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (273.68, 134.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (519, 768, 1023) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2031) [M::mem_pestat] mean and std.dev: (741.18, 355.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2535) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 337, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008) [M::mem_pestat] mean and std.dev: (360.16, 209.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1270) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696234 reads in 168.521 CPU sec, 5.427 real sec [M::mem_process_seqs] Processed 695954 reads in 108.360 CPU sec, 3.442 real sec sorting block 80, #reads = 1083950 mem = 536880853 peak = 1097494528 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 288133, 16, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 329, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (330.02, 200.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1171) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 251, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (273.59, 134.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (464, 682, 1571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3785) [M::mem_pestat] mean and std.dev: (965.62, 809.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4892) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 311, 714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1722) [M::mem_pestat] mean and std.dev: (451.59, 354.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2226) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 709988 sequences (100000033 bp) in 2.592 CPU sec, 2.684 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 287912, 23, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 268, 651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1635) [M::mem_pestat] mean and std.dev: (298.63, 250.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2127) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (276.08, 137.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (352, 609, 2433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6595) [M::mem_pestat] mean and std.dev: (1325.22, 1740.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8676) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 335, 604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1426) [M::mem_pestat] mean and std.dev: (321.18, 191.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1837) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 707516 sequences (100000079 bp) in 1.910 CPU sec, 1.911 real sec... [M::mem_process_seqs] Processed 695920 reads in 165.948 CPU sec, 5.263 real sec [M::mem_process_seqs] Processed 695924 reads in 111.257 CPU sec, 3.512 real sec sorting block 81, #reads = 1084818 mem = 536878951 peak = 1097494528 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 292496, 16, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 435, 773) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1969) [M::mem_pestat] mean and std.dev: (489.81, 432.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 211, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (235.43, 117.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (359, 1163, 2756) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7550) [M::mem_pestat] mean and std.dev: (2047.44, 2591.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12412) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 293, 541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1269) [M::mem_pestat] mean and std.dev: (377.19, 293.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1633) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 706826 sequences (100000236 bp) in 2.505 CPU sec, 2.607 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 292594, 27, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2671) [M::mem_pestat] mean and std.dev: (449.47, 475.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 219, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (245.90, 125.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (464, 799, 1083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2321) [M::mem_pestat] mean and std.dev: (665.58, 429.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2940) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 311, 824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2034) [M::mem_pestat] mean and std.dev: (477.72, 414.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2639) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 706858 sequences (100000274 bp) in 1.934 CPU sec, 1.935 real sec... [M::mem_process_seqs] Processed 709988 reads in 181.960 CPU sec, 5.784 real sec sorting block 82, #reads = 1081998 mem = 536877926 peak = 1097494528 [M::mem_process_seqs] Processed 707516 reads in 113.539 CPU sec, 3.597 real sec sorting block 83, #reads = 1089445 mem = 536881448 peak = 1097494528 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 292192, 16, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 293, 506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056) [M::mem_pestat] mean and std.dev: (375.49, 204.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1331) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 219, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (247.09, 126.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (312, 843, 1465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3771) [M::mem_pestat] mean and std.dev: (856.56, 747.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4924) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 301, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018) [M::mem_pestat] mean and std.dev: (298.06, 182.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1303) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 705216 sequences (100000016 bp) in 2.660 CPU sec, 2.779 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 292331, 22, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 286, 639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1571) [M::mem_pestat] mean and std.dev: (423.21, 369.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2037) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 220, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (247.32, 125.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (184, 383, 1335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3637) [M::mem_pestat] mean and std.dev: (710.14, 729.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4788) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 296, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956) [M::mem_pestat] mean and std.dev: (301.00, 145.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1203) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703742 sequences (100000036 bp) in 1.661 CPU sec, 1.669 real sec... [M::mem_process_seqs] Processed 706826 reads in 174.209 CPU sec, 5.538 real sec [M::mem_process_seqs] Processed 706858 reads in 113.039 CPU sec, 3.576 real sec sorting block 84, #reads = 1090742 mem = 536874577 peak = 1097494528 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 291572, 20, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 234, 648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1614) [M::mem_pestat] mean and std.dev: (361.44, 341.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2097) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 223, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (250.76, 127.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (911, 1385, 1453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2537) [M::mem_pestat] mean and std.dev: (1035.28, 498.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3079) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 256, 762) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1940) [M::mem_pestat] mean and std.dev: (387.50, 351.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2529) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703352 sequences (100000161 bp) in 2.536 CPU sec, 2.629 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 291584, 19, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 283, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (266.63, 128.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1077) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 228, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (255.71, 130.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (552, 879, 1349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2943) [M::mem_pestat] mean and std.dev: (796.29, 463.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3740) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 230, 603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1491) [M::mem_pestat] mean and std.dev: (230.30, 130.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1935) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702698 sequences (100000143 bp) in 1.899 CPU sec, 1.899 real sec... [M::mem_process_seqs] Processed 705216 reads in 170.504 CPU sec, 5.417 real sec [M::mem_process_seqs] Processed 703742 reads in 109.580 CPU sec, 3.464 real sec sorting block 85, #reads = 1089014 mem = 536879159 peak = 1097494528 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 290951, 10, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 228, 539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1295) [M::mem_pestat] mean and std.dev: (303.48, 244.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 229, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (257.57, 131.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (122, 654, 979) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2693) [M::mem_pestat] mean and std.dev: (618.00, 669.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3550) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 352, 656) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1494) [M::mem_pestat] mean and std.dev: (443.94, 322.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1913) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702588 sequences (100000210 bp) in 2.544 CPU sec, 2.674 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 290777, 23, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 272, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1081) [M::mem_pestat] mean and std.dev: (313.38, 201.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1380) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 231, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (259.96, 133.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (97, 449, 1280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3646) [M::mem_pestat] mean and std.dev: (853.36, 1008.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4889) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 332, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1130) [M::mem_pestat] mean and std.dev: (305.02, 183.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1448) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701510 sequences (100000095 bp) in 1.974 CPU sec, 1.979 real sec... [M::mem_process_seqs] Processed 703352 reads in 170.821 CPU sec, 5.430 real sec sorting block 86, #reads = 1090059 mem = 536882577 peak = 1097494528 [M::mem_process_seqs] Processed 702698 reads in 112.505 CPU sec, 3.838 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 290948, 8, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 438, 846) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2134) [M::mem_pestat] mean and std.dev: (610.58, 494.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2778) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 233, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (261.58, 134.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 319, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1120) [M::mem_pestat] mean and std.dev: (300.14, 164.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1437) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 87, #reads = 1087831 mem = 536878221 peak = 1097494528 [M::process] read 701446 sequences (100000214 bp) in 2.757 CPU sec, 2.896 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 290062, 18, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 271, 639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1581) [M::mem_pestat] mean and std.dev: (322.58, 254.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2052) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 235, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (263.42, 135.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (195, 319, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1077) [M::mem_pestat] mean and std.dev: (291.81, 134.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1371) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (257, 325, 641) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1409) [M::mem_pestat] mean and std.dev: (395.20, 250.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1793) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701348 sequences (100000218 bp) in 1.951 CPU sec, 1.957 real sec... [M::mem_process_seqs] Processed 702588 reads in 170.071 CPU sec, 5.662 real sec [M::mem_process_seqs] Processed 701510 reads in 108.245 CPU sec, 3.411 real sec sorting block 88, #reads = 1086731 mem = 536879634 peak = 1097494528 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 289793, 43, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 226, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (234.74, 95.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 236, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (264.18, 135.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (667, 924, 1173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2185) [M::mem_pestat] mean and std.dev: (822.76, 354.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2691) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 361, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1097) [M::mem_pestat] mean and std.dev: (365.38, 243.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1398) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700752 sequences (100000131 bp) in 2.681 CPU sec, 2.781 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 290156, 25, 72) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 257, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (259.37, 110.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1062) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 236, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (264.41, 135.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (178, 342, 1343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3673) [M::mem_pestat] mean and std.dev: (425.86, 390.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4838) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 341, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1097) [M::mem_pestat] mean and std.dev: (349.58, 220.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1398) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700624 sequences (100000023 bp) in 1.927 CPU sec, 1.935 real sec... [M::mem_process_seqs] Processed 701446 reads in 166.652 CPU sec, 5.283 real sec [M::mem_process_seqs] Processed 701348 reads in 109.797 CPU sec, 3.462 real sec sorting block 89, #reads = 1087871 mem = 536879619 peak = 1097494528 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 290429, 26, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 349, 666) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1672) [M::mem_pestat] mean and std.dev: (370.06, 260.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2175) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 239, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (267.79, 138.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1131, 1701, 4074) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9960) [M::mem_pestat] mean and std.dev: (2192.77, 1665.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12903) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 307, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1178) [M::mem_pestat] mean and std.dev: (366.21, 229.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1500) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701178 sequences (100000217 bp) in 2.685 CPU sec, 2.806 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 290168, 19, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 258, 1067) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2845) [M::mem_pestat] mean and std.dev: (553.76, 615.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 238, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (267.31, 137.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (170, 327, 718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1814) [M::mem_pestat] mean and std.dev: (430.18, 433.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2362) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 304, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996) [M::mem_pestat] mean and std.dev: (313.85, 181.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1257) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700752 reads in 165.271 CPU sec, 5.248 real sec [M::process] read 699438 sequences (100000169 bp) in 2.047 CPU sec, 2.073 real sec... [M::mem_process_seqs] Processed 700624 reads in 109.926 CPU sec, 3.458 real sec sorting block 90, #reads = 1086574 mem = 536880043 peak = 1097494528 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 290208, 19, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 187, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (196.79, 83.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 237, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (266.62, 138.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (500, 969, 2655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6965) [M::mem_pestat] mean and std.dev: (1814.42, 1549.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9120) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 276, 1001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2545) [M::mem_pestat] mean and std.dev: (546.88, 526.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3317) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698766 sequences (100000014 bp) in 2.704 CPU sec, 2.841 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 289258, 22, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (252, 482, 998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2490) [M::mem_pestat] mean and std.dev: (640.28, 570.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3236) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 241, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (268.49, 136.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (484, 1115, 1387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3193) [M::mem_pestat] mean and std.dev: (961.57, 714.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4096) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 256, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1248) [M::mem_pestat] mean and std.dev: (290.18, 232.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1608) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698556 sequences (100000065 bp) in 1.770 CPU sec, 1.777 real sec... [M::mem_process_seqs] Processed 701178 reads in 172.313 CPU sec, 5.686 real sec sorting block 91, #reads = 1087982 mem = 536884197 peak = 1097494528 [M::mem_process_seqs] Processed 699438 reads in 111.194 CPU sec, 3.508 real sec sorting block 92, #reads = 1087462 mem = 536881825 peak = 1155514368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 289263, 21, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 362, 732) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1844) [M::mem_pestat] mean and std.dev: (464.32, 401.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2400) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 242, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (269.01, 136.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (579, 1083, 1447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3183) [M::mem_pestat] mean and std.dev: (885.74, 441.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4051) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 271, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (309.57, 195.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1194) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698196 sequences (100000114 bp) in 3.000 CPU sec, 3.157 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 288988, 29, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 261, 567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1325) [M::mem_pestat] mean and std.dev: (354.69, 228.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1704) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 242, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (269.02, 135.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (221, 484, 1924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5330) [M::mem_pestat] mean and std.dev: (1007.62, 931.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7033) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 222, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (233.05, 99.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697760 sequences (100000288 bp) in 1.882 CPU sec, 1.885 real sec... [M::mem_process_seqs] Processed 698766 reads in 170.710 CPU sec, 5.409 real sec [M::mem_process_seqs] Processed 698556 reads in 114.197 CPU sec, 3.608 real sec sorting block 93, #reads = 1083998 mem = 536877847 peak = 1155514368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 289138, 25, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 293, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (283.05, 102.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 243, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (270.19, 137.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (434, 668, 1385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3287) [M::mem_pestat] mean and std.dev: (856.09, 763.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4238) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 259, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (243.84, 98.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698072 sequences (100000232 bp) in 2.649 CPU sec, 2.775 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 288787, 18, 73) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 334, 586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1382) [M::mem_pestat] mean and std.dev: (332.51, 214.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (269.96, 135.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (262, 450, 1096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2764) [M::mem_pestat] mean and std.dev: (543.62, 589.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3598) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 325, 725) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1771) [M::mem_pestat] mean and std.dev: (333.02, 204.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2294) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698194 sequences (100000240 bp) in 1.888 CPU sec, 1.890 real sec... [M::mem_process_seqs] Processed 698196 reads in 172.763 CPU sec, 5.554 real sec [M::mem_process_seqs] Processed 697760 reads in 110.319 CPU sec, 3.483 real sec sorting block 94, #reads = 1083556 mem = 536881036 peak = 1155514368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 288789, 27, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 292, 713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1821) [M::mem_pestat] mean and std.dev: (440.75, 351.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 243, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (269.75, 135.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (443, 1057, 2387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6275) [M::mem_pestat] mean and std.dev: (1304.12, 1419.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8219) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 313, 688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1626) [M::mem_pestat] mean and std.dev: (395.43, 246.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2095) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698078 sequences (100000214 bp) in 2.676 CPU sec, 2.792 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 289133, 20, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 339, 625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1505) [M::mem_pestat] mean and std.dev: (387.98, 329.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1945) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 242, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (269.16, 136.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (64, 274, 922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2638) [M::mem_pestat] mean and std.dev: (467.90, 519.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3496) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 306, 643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1483) [M::mem_pestat] mean and std.dev: (397.07, 306.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1903) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699046 sequences (100000171 bp) in 1.870 CPU sec, 1.871 real sec... [M::mem_process_seqs] Processed 698072 reads in 170.114 CPU sec, 5.397 real sec sorting block 95, #reads = 1085201 mem = 536877837 peak = 1155514368 [M::mem_process_seqs] Processed 698194 reads in 112.662 CPU sec, 3.553 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 288691, 13, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 301, 1009) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2705) [M::mem_pestat] mean and std.dev: (523.61, 460.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 242, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (267.99, 134.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (525, 1172, 3010) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7980) [M::mem_pestat] mean and std.dev: (1601.54, 1650.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10465) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 290, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007) [M::mem_pestat] mean and std.dev: (288.81, 151.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1270) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 96, #reads = 1083937 mem = 536877265 peak = 1155514368 [M::process] read 698936 sequences (100000142 bp) in 2.492 CPU sec, 2.617 real sec... [M::process] read 698164 sequences (100000107 bp) in 1.460 CPU sec, 1.460 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 288798, 27, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 332, 1039) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2701) [M::mem_pestat] mean and std.dev: (618.42, 550.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 240, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (266.66, 134.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (166, 1163, 3210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9298) [M::mem_pestat] mean and std.dev: (1649.56, 1746.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12342) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 306, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (296.88, 108.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698078 reads in 170.919 CPU sec, 5.442 real sec [M::mem_process_seqs] Processed 699046 reads in 112.824 CPU sec, 3.579 real sec sorting block 97, #reads = 1082457 mem = 536880450 peak = 1155514368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 289264, 20, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 278, 1202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3286) [M::mem_pestat] mean and std.dev: (687.29, 815.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4328) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 240, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (266.61, 134.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (73, 550, 1126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3232) [M::mem_pestat] mean and std.dev: (678.60, 735.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4285) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 355, 725) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1733) [M::mem_pestat] mean and std.dev: (373.06, 288.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2237) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697836 sequences (100000238 bp) in 2.836 CPU sec, 2.969 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 288641, 21, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 312, 1932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5488) [M::mem_pestat] mean and std.dev: (859.85, 1056.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7266) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 242, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (268.48, 135.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (425, 931, 2833) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7649) [M::mem_pestat] mean and std.dev: (1178.90, 1106.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10057) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 323, 996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2594) [M::mem_pestat] mean and std.dev: (539.79, 455.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3393) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697546 sequences (100000210 bp) in 1.849 CPU sec, 1.846 real sec... [M::mem_process_seqs] Processed 698936 reads in 171.460 CPU sec, 5.444 real sec [M::mem_process_seqs] Processed 698164 reads in 114.440 CPU sec, 3.612 real sec sorting block 98, #reads = 1083971 mem = 536879135 peak = 1155514368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 287950, 24, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 225, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1111) [M::mem_pestat] mean and std.dev: (275.47, 198.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 243, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (269.17, 135.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (423, 1683, 2291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6027) [M::mem_pestat] mean and std.dev: (1807.42, 1624.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8307) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 251, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (251.75, 119.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697716 sequences (100000043 bp) in 2.990 CPU sec, 3.095 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 288909, 30, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 225, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (279.19, 176.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1090) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 244, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (269.60, 134.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (188, 689, 1805) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5039) [M::mem_pestat] mean and std.dev: (720.08, 669.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6656) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 218, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1165) [M::mem_pestat] mean and std.dev: (294.50, 202.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1494) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697902 sequences (100000243 bp) in 1.900 CPU sec, 1.905 real sec... [M::mem_process_seqs] Processed 697836 reads in 171.557 CPU sec, 5.436 real sec sorting block 99, #reads = 1082831 mem = 536877236 peak = 1155514368 [M::mem_process_seqs] Processed 697546 reads in 113.428 CPU sec, 3.580 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 288586, 14, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 229, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (244.47, 120.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 243, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (269.06, 135.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (495, 999, 1627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3891) [M::mem_pestat] mean and std.dev: (1107.38, 946.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5023) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 285, 535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1189) [M::mem_pestat] mean and std.dev: (315.49, 145.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1516) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 100, #reads = 1082495 mem = 536878411 peak = 1155514368 [M::process] read 708644 sequences (100000245 bp) in 3.018 CPU sec, 3.109 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 288948, 17, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 223, 478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1104) [M::mem_pestat] mean and std.dev: (330.53, 247.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 243, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (269.54, 135.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (72, 487, 912) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2592) [M::mem_pestat] mean and std.dev: (573.12, 581.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3432) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 272, 623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1481) [M::mem_pestat] mean and std.dev: (298.69, 176.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1910) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 710062 sequences (100000264 bp) in 1.930 CPU sec, 1.931 real sec... [M::mem_process_seqs] Processed 697716 reads in 179.184 CPU sec, 5.678 real sec [M::mem_process_seqs] Processed 697902 reads in 120.509 CPU sec, 3.805 real sec sorting block 101, #reads = 1082375 mem = 536879767 peak = 1155514368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 292365, 24, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 272, 541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1249) [M::mem_pestat] mean and std.dev: (285.91, 184.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 215, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (241.33, 121.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (284, 830, 1435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3737) [M::mem_pestat] mean and std.dev: (901.39, 828.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4888) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 312, 776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1990) [M::mem_pestat] mean and std.dev: (416.94, 361.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2597) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 708066 sequences (100000082 bp) in 2.831 CPU sec, 2.923 real sec... [M::process] read 707928 sequences (100000148 bp) in 2.034 CPU sec, 2.036 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 293227, 14, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 277, 973) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2539) [M::mem_pestat] mean and std.dev: (481.74, 502.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3322) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 212, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (238.04, 119.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (209, 550, 1356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3650) [M::mem_pestat] mean and std.dev: (552.50, 461.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4797) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 318, 939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2377) [M::mem_pestat] mean and std.dev: (464.08, 378.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3096) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 708644 reads in 198.290 CPU sec, 6.293 real sec [M::mem_process_seqs] Processed 710062 reads in 129.066 CPU sec, 4.092 real sec sorting block 102, #reads = 1081962 mem = 536880161 peak = 1155514368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 292945, 11, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 306, 839) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2193) [M::mem_pestat] mean and std.dev: (429.10, 458.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2870) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 217, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (243.13, 122.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1215, 1300, 3612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8406) [M::mem_pestat] mean and std.dev: (2469.91, 2272.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11558) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 391, 675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1631) [M::mem_pestat] mean and std.dev: (452.46, 356.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2109) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 707546 sequences (100000100 bp) in 2.700 CPU sec, 2.773 real sec... [M::process] read 706212 sequences (100000026 bp) in 2.040 CPU sec, 2.044 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 292588, 19, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 299, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1100) [M::mem_pestat] mean and std.dev: (308.85, 184.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1406) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 217, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (242.98, 122.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (272, 512, 2201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6059) [M::mem_pestat] mean and std.dev: (1125.68, 1153.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7988) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 357, 697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1689) [M::mem_pestat] mean and std.dev: (426.81, 275.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2185) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 708066 reads in 192.100 CPU sec, 6.385 real sec [M::mem_process_seqs] Processed 707928 reads in 124.535 CPU sec, 3.944 real sec sorting block 103, #reads = 1085749 mem = 536878224 peak = 1155514368 [M::process] read 705442 sequences (100000281 bp) in 2.739 CPU sec, 2.824 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 292192, 12, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 246, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (283.17, 156.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1177) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 705026 sequences (100000174 bp) in 2.020 CPU sec, 2.024 real sec... [M::mem_pestat] (25, 50, 75) percentile: (150, 222, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (247.91, 124.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (446, 463, 555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (228, 773) [M::mem_pestat] mean and std.dev: (489.22, 55.45) [M::mem_pestat] low and high boundaries for proper pairs: (119, 882) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 272, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958) [M::mem_pestat] mean and std.dev: (306.73, 149.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1213) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 706212 reads in 104.193 CPU sec, 3.280 real sec sorting block 104, #reads = 1084684 mem = 536877910 peak = 1155514368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 292710, 19, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 345, 1204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3272) [M::mem_pestat] mean and std.dev: (746.16, 877.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4306) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 218, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (244.24, 122.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (494, 825, 2270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5822) [M::mem_pestat] mean and std.dev: (1160.05, 1002.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7598) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 272, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (293.00, 167.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 291789, 15, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 231, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (248.02, 150.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1067) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 223, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (249.66, 125.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (143, 396, 1123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3083) [M::mem_pestat] mean and std.dev: (631.67, 533.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4063) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 261, 1172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3176) [M::mem_pestat] mean and std.dev: (726.72, 834.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4178) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 707546 reads in 291.138 CPU sec, 9.227 real sec [M::process] read 704588 sequences (100000205 bp) in 2.416 CPU sec, 2.419 real sec... [M::mem_process_seqs] Processed 705442 reads in 144.275 CPU sec, 4.596 real sec sorting block 105, #reads = 1083951 mem = 536883464 peak = 1158754304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 291231, 20, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 334, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986) [M::mem_pestat] mean and std.dev: (342.38, 232.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1273) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 225, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (252.00, 127.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (506, 1131, 2036) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5096) [M::mem_pestat] mean and std.dev: (830.81, 508.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6626) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 252, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038) [M::mem_pestat] mean and std.dev: (272.74, 161.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1330) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703496 sequences (100000245 bp) in 2.916 CPU sec, 3.056 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 291388, 18, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 249, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1135) [M::mem_pestat] mean and std.dev: (286.29, 174.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 226, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (253.15, 128.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (503, 1427, 3990) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10964) [M::mem_pestat] mean and std.dev: (1971.78, 1611.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14451) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 375, 654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1494) [M::mem_pestat] mean and std.dev: (406.70, 248.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1914) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702506 sequences (100000009 bp) in 1.972 CPU sec, 1.980 real sec... [M::mem_process_seqs] Processed 705026 reads in 183.464 CPU sec, 5.824 real sec sorting block 106, #reads = 1083966 mem = 536880985 peak = 1158754304 [M::mem_process_seqs] Processed 704588 reads in 119.740 CPU sec, 3.788 real sec sorting block 107, #reads = 1084766 mem = 536881670 peak = 1158754304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 291463, 17, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 244, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1164) [M::mem_pestat] mean and std.dev: (330.54, 250.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 229, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (255.81, 129.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (148, 232, 665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1699) [M::mem_pestat] mean and std.dev: (387.88, 374.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2216) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 346, 595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1375) [M::mem_pestat] mean and std.dev: (362.10, 198.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1765) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701894 sequences (100000053 bp) in 2.923 CPU sec, 3.101 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 291081, 13, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (200.31, 87.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 231, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (257.83, 130.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (536, 682, 1166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2426) [M::mem_pestat] mean and std.dev: (791.75, 425.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3056) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 358, 870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2234) [M::mem_pestat] mean and std.dev: (435.51, 352.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2916) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701912 sequences (100000064 bp) in 1.978 CPU sec, 1.981 real sec... [M::mem_process_seqs] Processed 703496 reads in 175.727 CPU sec, 5.580 real sec [M::mem_process_seqs] Processed 702506 reads in 113.911 CPU sec, 3.901 real sec sorting block 108, #reads = 1085956 mem = 536876237 peak = 1158754304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 290496, 22, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 305, 852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2152) [M::mem_pestat] mean and std.dev: (477.27, 492.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2802) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 232, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (259.29, 131.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (126, 662, 1944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5580) [M::mem_pestat] mean and std.dev: (992.63, 1365.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7398) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 264, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1058) [M::mem_pestat] mean and std.dev: (316.68, 199.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1343) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701428 sequences (100000156 bp) in 2.650 CPU sec, 2.780 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 290411, 20, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 230, 615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1567) [M::mem_pestat] mean and std.dev: (325.55, 280.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2043) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 232, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (259.25, 131.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (560, 1286, 2635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6785) [M::mem_pestat] mean and std.dev: (1601.00, 1504.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8860) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 310, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (282.18, 113.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701636 sequences (100000284 bp) in 1.945 CPU sec, 1.949 real sec... [M::mem_process_seqs] Processed 701894 reads in 178.823 CPU sec, 5.982 real sec [M::mem_process_seqs] Processed 701912 reads in 116.287 CPU sec, 3.712 real sec sorting block 109, #reads = 1085676 mem = 536880218 peak = 1158754304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 290090, 15, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 246, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (269.35, 152.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 234, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (261.66, 132.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (186, 502, 2306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6546) [M::mem_pestat] mean and std.dev: (1159.07, 1462.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8666) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 263, 732) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1862) [M::mem_pestat] mean and std.dev: (329.78, 296.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2427) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700828 sequences (100000143 bp) in 2.630 CPU sec, 2.771 real sec... [M::process] read 700472 sequences (100000033 bp) in 1.590 CPU sec, 1.595 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 290345, 11, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 297, 1152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3090) [M::mem_pestat] mean and std.dev: (544.59, 641.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4059) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 233, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (261.12, 133.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 908) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (893, 2230, 2781) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6557) [M::mem_pestat] mean and std.dev: (1958.09, 1395.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8445) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 295, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050) [M::mem_pestat] mean and std.dev: (307.79, 183.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1341) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701428 reads in 173.239 CPU sec, 5.508 real sec sorting block 110, #reads = 1085485 mem = 536882298 peak = 1158754304 [M::mem_process_seqs] Processed 701636 reads in 112.204 CPU sec, 3.561 real sec sorting block 111, #reads = 1085063 mem = 536885280 peak = 1158754304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 290040, 21, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 278, 669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1697) [M::mem_pestat] mean and std.dev: (355.50, 342.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2211) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 236, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (264.18, 134.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (240, 781, 1419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3777) [M::mem_pestat] mean and std.dev: (910.45, 985.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4956) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 209, 582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1450) [M::mem_pestat] mean and std.dev: (333.14, 299.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1884) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699476 sequences (100000226 bp) in 2.581 CPU sec, 2.684 real sec... [M::process] read 699422 sequences (100000063 bp) in 1.948 CPU sec, 1.953 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 289941, 16, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 309, 807) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2021) [M::mem_pestat] mean and std.dev: (443.23, 471.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 236, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (261.87, 131.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (125, 776, 1149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3197) [M::mem_pestat] mean and std.dev: (797.25, 668.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4221) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 309, 569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1283) [M::mem_pestat] mean and std.dev: (342.60, 218.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1640) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700828 reads in 169.633 CPU sec, 5.675 real sec [M::mem_process_seqs] Processed 700472 reads in 111.322 CPU sec, 3.518 real sec sorting block 112, #reads = 1085673 mem = 536877241 peak = 1158754304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 289740, 11, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 358, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075) [M::mem_pestat] mean and std.dev: (321.65, 223.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1381) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 239, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (266.64, 135.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (547, 2222, 7796) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 22294) [M::mem_pestat] mean and std.dev: (3800.91, 3536.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 29543) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 358, 735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1801) [M::mem_pestat] mean and std.dev: (436.00, 331.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2334) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698648 sequences (100000221 bp) in 2.631 CPU sec, 2.737 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 289482, 13, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 266, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042) [M::mem_pestat] mean and std.dev: (282.44, 145.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1335) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 240, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (267.06, 135.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (138, 1123, 3083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8973) [M::mem_pestat] mean and std.dev: (1528.85, 1668.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11918) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (277, 426, 707) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1567) [M::mem_pestat] mean and std.dev: (440.89, 245.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1997) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698384 sequences (100000109 bp) in 1.752 CPU sec, 1.760 real sec... [M::mem_process_seqs] Processed 699476 reads in 170.534 CPU sec, 5.411 real sec [M::mem_process_seqs] Processed 699422 reads in 113.387 CPU sec, 3.590 real sec sorting block 113, #reads = 1085069 mem = 536880340 peak = 1158754304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 289068, 19, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 336, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1244) [M::mem_pestat] mean and std.dev: (327.79, 215.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 241, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (267.66, 135.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (484, 491, 1957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4903) [M::mem_pestat] mean and std.dev: (1108.21, 1141.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6376) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 331, 1135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2939) [M::mem_pestat] mean and std.dev: (569.34, 514.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3841) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698578 sequences (100000206 bp) in 2.697 CPU sec, 2.855 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 289176, 18, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 234, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (232.23, 109.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 912) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 241, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (268.14, 135.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (302, 499, 1822) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4862) [M::mem_pestat] mean and std.dev: (964.44, 952.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6382) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 272, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (267.48, 130.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698722 sequences (100000119 bp) in 1.837 CPU sec, 1.839 real sec... [M::mem_process_seqs] Processed 698648 reads in 174.794 CPU sec, 5.561 real sec sorting block 114, #reads = 1083180 mem = 536878219 peak = 1158754304 [M::mem_process_seqs] Processed 698384 reads in 126.271 CPU sec, 4.020 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 289308, 12, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 226, 848) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2258) [M::mem_pestat] mean and std.dev: (422.07, 409.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2963) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 242, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (269.06, 136.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (622, 876, 3467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9157) [M::mem_pestat] mean and std.dev: (1995.92, 2358.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12002) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 280, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001) [M::mem_pestat] mean and std.dev: (316.13, 152.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1254) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 115, #reads = 1084040 mem = 536880577 peak = 1158754304 [M::process] read 698686 sequences (100000141 bp) in 2.646 CPU sec, 2.768 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 289471, 18, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 316, 852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2212) [M::mem_pestat] mean and std.dev: (525.92, 515.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2892) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 240, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (267.74, 135.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (598, 1078, 1301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2707) [M::mem_pestat] mean and std.dev: (868.88, 449.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3410) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 339, 816) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2004) [M::mem_pestat] mean and std.dev: (431.06, 355.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2598) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698472 sequences (100000170 bp) in 1.628 CPU sec, 1.630 real sec... [M::mem_process_seqs] Processed 698578 reads in 169.858 CPU sec, 5.414 real sec [M::mem_process_seqs] Processed 698722 reads in 118.825 CPU sec, 3.788 real sec sorting block 116, #reads = 1085169 mem = 536878001 peak = 1158754304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 288357, 18, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 226, 1065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2853) [M::mem_pestat] mean and std.dev: (461.85, 512.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 241, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (268.27, 136.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (230, 778, 2097) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5831) [M::mem_pestat] mean and std.dev: (1037.22, 917.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7698) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (248, 337, 593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1283) [M::mem_pestat] mean and std.dev: (346.06, 200.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1628) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698756 sequences (100000283 bp) in 2.620 CPU sec, 2.727 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 288961, 8, 83) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 354, 698) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1726) [M::mem_pestat] mean and std.dev: (428.72, 290.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2240) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 241, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (268.60, 136.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 353, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1110) [M::mem_pestat] mean and std.dev: (324.67, 162.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1414) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 698556 sequences (100000045 bp) in 1.790 CPU sec, 1.796 real sec... [M::mem_process_seqs] Processed 698686 reads in 168.367 CPU sec, 5.344 real sec [M::mem_process_seqs] Processed 698472 reads in 109.240 CPU sec, 3.446 real sec sorting block 117, #reads = 1083510 mem = 536880129 peak = 1158754304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 289357, 11, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 303, 678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1676) [M::mem_pestat] mean and std.dev: (401.28, 358.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2175) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 239, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (267.55, 136.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 138, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1515) [M::mem_pestat] mean and std.dev: (190.10, 205.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2014) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 454, 796) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1930) [M::mem_pestat] mean and std.dev: (498.80, 354.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2497) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698164 sequences (100000204 bp) in 2.890 CPU sec, 3.014 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 288912, 11, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 290, 793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2049) [M::mem_pestat] mean and std.dev: (465.38, 449.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 241, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (269.06, 137.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (365, 390, 744) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1502) [M::mem_pestat] mean and std.dev: (435.11, 230.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1881) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (245, 296, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971) [M::mem_pestat] mean and std.dev: (340.17, 185.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1213) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698756 reads in 170.619 CPU sec, 5.455 real sec [M::process] read 698924 sequences (100000081 bp) in 2.161 CPU sec, 2.176 real sec... [M::mem_process_seqs] Processed 698556 reads in 111.413 CPU sec, 3.503 real sec sorting block 118, #reads = 1084081 mem = 536878595 peak = 1158754304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 288700, 10, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 296, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1286) [M::mem_pestat] mean and std.dev: (321.12, 208.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 242, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (270.45, 138.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (72, 202, 689) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1923) [M::mem_pestat] mean and std.dev: (302.30, 284.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2540) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 279, 720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1808) [M::mem_pestat] mean and std.dev: (387.37, 367.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2352) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 707520 sequences (100000077 bp) in 2.787 CPU sec, 2.895 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 288731, 18, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 266, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (259.81, 145.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 239, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (266.04, 135.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (321, 442, 1458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3732) [M::mem_pestat] mean and std.dev: (883.94, 870.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4869) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 318, 657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1527) [M::mem_pestat] mean and std.dev: (440.82, 364.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1962) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 707578 sequences (100000119 bp) in 1.950 CPU sec, 1.954 real sec... [M::mem_process_seqs] Processed 698164 reads in 183.200 CPU sec, 5.811 real sec sorting block 119, #reads = 1082699 mem = 536877318 peak = 1158754304 [M::mem_process_seqs] Processed 698924 reads in 132.353 CPU sec, 4.194 real sec sorting block 120, #reads = 1079673 mem = 536880486 peak = 1158754304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 292202, 21, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 260, 889) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2361) [M::mem_pestat] mean and std.dev: (428.66, 370.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3097) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 219, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (244.99, 123.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (163, 796, 1329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3661) [M::mem_pestat] mean and std.dev: (954.24, 890.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4827) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 257, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833) [M::mem_pestat] mean and std.dev: (282.91, 148.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 707284 sequences (100000297 bp) in 2.835 CPU sec, 2.966 real sec... [M::process] read 706306 sequences (100000009 bp) in 1.510 CPU sec, 1.512 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 292113, 19, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 204, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (203.87, 84.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 218, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (244.33, 123.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (683, 1398, 1891) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4307) [M::mem_pestat] mean and std.dev: (1089.44, 834.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5515) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 280, 742) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1932) [M::mem_pestat] mean and std.dev: (442.58, 371.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2527) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 707520 reads in 171.246 CPU sec, 5.438 real sec [M::mem_process_seqs] Processed 707578 reads in 109.482 CPU sec, 3.755 real sec sorting block 121, #reads = 1081060 mem = 536877953 peak = 1158754304 [M::process] read 705270 sequences (100000019 bp) in 2.535 CPU sec, 2.649 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 292082, 10, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 291, 635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1539) [M::mem_pestat] mean and std.dev: (314.42, 219.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1991) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 221, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (248.00, 125.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (280, 537, 898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2134) [M::mem_pestat] mean and std.dev: (638.30, 494.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2752) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 308, 797) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1985) [M::mem_pestat] mean and std.dev: (414.81, 347.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2579) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704686 sequences (100000017 bp) in 1.910 CPU sec, 1.911 real sec... [M::mem_process_seqs] Processed 706306 reads in 95.905 CPU sec, 3.037 real sec sorting block 122, #reads = 1089140 mem = 536874678 peak = 1158754304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 292444, 18, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 281, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1096) [M::mem_pestat] mean and std.dev: (293.49, 147.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 219, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (244.83, 123.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (3, 766, 766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2292) [M::mem_pestat] mean and std.dev: (524.82, 472.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3055) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 362, 564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1264) [M::mem_pestat] mean and std.dev: (388.87, 278.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1614) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 291789, 22, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 321, 990) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2628) [M::mem_pestat] mean and std.dev: (411.81, 372.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 224, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (250.43, 127.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (404, 737, 1688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4256) [M::mem_pestat] mean and std.dev: (968.57, 988.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5540) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 275, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1163) [M::mem_pestat] mean and std.dev: (309.56, 195.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1492) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 707284 reads in 258.616 CPU sec, 8.479 real sec [M::process] read 704228 sequences (100000085 bp) in 2.249 CPU sec, 2.273 real sec... [M::mem_process_seqs] Processed 705270 reads in 125.438 CPU sec, 3.985 real sec sorting block 123, #reads = 1088871 mem = 536877375 peak = 1158754304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 291842, 18, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 292, 611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1537) [M::mem_pestat] mean and std.dev: (264.27, 159.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2000) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 225, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (252.59, 128.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (147, 708, 1778) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5040) [M::mem_pestat] mean and std.dev: (1276.33, 1489.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7235) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (238, 306, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985) [M::mem_pestat] mean and std.dev: (313.94, 155.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1234) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702872 sequences (100000166 bp) in 3.104 CPU sec, 3.288 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 291187, 9, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 204, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (199.39, 104.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 227, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (254.00, 129.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 260, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1098) [M::mem_pestat] mean and std.dev: (325.85, 208.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1405) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 704686 reads in 162.857 CPU sec, 5.180 real sec [M::mem_process_seqs] Processed 704228 reads in 108.304 CPU sec, 3.408 real sec [M::process] read 701942 sequences (100000069 bp) in 2.251 CPU sec, 2.341 real sec... sorting block 124, #reads = 1089963 mem = 536883101 peak = 1158754304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 290994, 8, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 213, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (228.64, 117.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1158) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 230, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (256.54, 129.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 438, 586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1298) [M::mem_pestat] mean and std.dev: (428.11, 275.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1654) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 702292 sequences (100000151 bp) in 2.671 CPU sec, 2.810 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 290373, 20, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 231, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (249.18, 143.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 233, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (259.26, 130.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (280, 723, 810) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1870) [M::mem_pestat] mean and std.dev: (621.21, 326.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2400) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 285, 582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1372) [M::mem_pestat] mean and std.dev: (363.83, 236.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1767) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702872 reads in 162.719 CPU sec, 5.162 real sec [M::process] read 701792 sequences (100000191 bp) in 2.116 CPU sec, 2.151 real sec... sorting block 125, #reads = 1089568 mem = 536878970 peak = 1158754304 [M::mem_process_seqs] Processed 701942 reads in 105.920 CPU sec, 3.334 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 290615, 13, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 238, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (222.41, 83.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 231, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (257.46, 130.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (341, 723, 5202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14924) [M::mem_pestat] mean and std.dev: (1987.46, 2071.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19785) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 262, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (252.48, 107.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 126, #reads = 1088549 mem = 536877131 peak = 1158754304 [M::process] read 701610 sequences (100000252 bp) in 2.876 CPU sec, 3.040 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 290456, 10, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 288, 1237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3333) [M::mem_pestat] mean and std.dev: (568.15, 652.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4381) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 234, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (260.37, 131.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (505, 1594, 7138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 20404) [M::mem_pestat] mean and std.dev: (3273.30, 3547.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 27037) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 267, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (283.31, 144.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702292 reads in 164.431 CPU sec, 5.235 real sec [M::process] read 701180 sequences (100000229 bp) in 2.077 CPU sec, 2.111 real sec... [M::mem_process_seqs] Processed 701792 reads in 113.760 CPU sec, 3.602 real sec sorting block 127, #reads = 1089489 mem = 536883423 peak = 1158754304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 290861, 13, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 248, 557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1427) [M::mem_pestat] mean and std.dev: (281.77, 250.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1862) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 233, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (259.74, 130.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (785, 1164, 2413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5669) [M::mem_pestat] mean and std.dev: (1324.58, 1076.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7297) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 292, 1042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2716) [M::mem_pestat] mean and std.dev: (532.49, 565.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3553) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701400 sequences (100000107 bp) in 2.676 CPU sec, 2.835 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 289927, 25, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 214, 867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2307) [M::mem_pestat] mean and std.dev: (418.97, 391.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3027) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 235, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (260.58, 130.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (437, 730, 1935) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4931) [M::mem_pestat] mean and std.dev: (956.17, 664.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6429) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 215, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1132) [M::mem_pestat] mean and std.dev: (302.93, 222.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1458) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701610 reads in 166.289 CPU sec, 5.292 real sec [M::process] read 701068 sequences (100000046 bp) in 1.986 CPU sec, 2.014 real sec... [M::mem_process_seqs] Processed 701180 reads in 115.017 CPU sec, 3.638 real sec sorting block 128, #reads = 1087176 mem = 536878826 peak = 1158754304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 290329, 18, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 294, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (287.86, 156.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 235, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (260.74, 131.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (96, 374, 951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2661) [M::mem_pestat] mean and std.dev: (655.29, 717.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3524) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 298, 623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1401) [M::mem_pestat] mean and std.dev: (407.41, 259.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1790) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701266 sequences (100000117 bp) in 2.280 CPU sec, 2.424 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 290067, 21, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 311, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (262.00, 92.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 236, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (261.62, 131.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (775, 1233, 8819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 24907) [M::mem_pestat] mean and std.dev: (3630.33, 3762.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 32951) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 373, 670) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1584) [M::mem_pestat] mean and std.dev: (465.52, 360.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2041) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701236 sequences (100000077 bp) in 1.601 CPU sec, 1.611 real sec... [M::mem_process_seqs] Processed 701400 reads in 168.659 CPU sec, 5.382 real sec sorting block 129, #reads = 1087637 mem = 536878613 peak = 1158754304 [M::mem_process_seqs] Processed 701068 reads in 111.513 CPU sec, 3.539 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 290318, 21, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 226, 728) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1834) [M::mem_pestat] mean and std.dev: (350.85, 357.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2387) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 234, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (260.94, 131.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (104, 464, 2339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6809) [M::mem_pestat] mean and std.dev: (1286.57, 1611.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9044) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 367, 738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1832) [M::mem_pestat] mean and std.dev: (354.98, 254.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2379) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 130, #reads = 1088370 mem = 536886382 peak = 1158754304 [M::process] read 701814 sequences (100000283 bp) in 3.393 CPU sec, 3.568 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 290724, 17, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 325, 597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1443) [M::mem_pestat] mean and std.dev: (381.89, 274.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1866) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 233, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (260.62, 131.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (379, 632, 929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2029) [M::mem_pestat] mean and std.dev: (670.18, 439.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2579) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 247, 540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1258) [M::mem_pestat] mean and std.dev: (341.50, 228.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1617) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701266 reads in 173.827 CPU sec, 5.531 real sec [M::mem_process_seqs] Processed 701236 reads in 113.915 CPU sec, 3.595 real sec [M::process] read 701836 sequences (100000037 bp) in 2.526 CPU sec, 2.604 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 290392, 20, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 394, 999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2619) [M::mem_pestat] mean and std.dev: (538.16, 568.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3429) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 231, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (257.19, 128.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (194, 1307, 1664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4604) [M::mem_pestat] mean and std.dev: (1009.89, 1094.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6074) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 444, 893) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2219) [M::mem_pestat] mean and std.dev: (554.45, 517.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2882) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 131, #reads = 1087405 mem = 536877086 peak = 1158754304 [M::process] read 702048 sequences (100000154 bp) in 2.810 CPU sec, 2.959 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 290288, 14, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 364, 623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1523) [M::mem_pestat] mean and std.dev: (341.24, 225.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1973) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 231, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (258.16, 130.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (390, 791, 1828) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4704) [M::mem_pestat] mean and std.dev: (1045.77, 842.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6142) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 296, 599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1469) [M::mem_pestat] mean and std.dev: (301.37, 240.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1904) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701814 reads in 175.526 CPU sec, 5.598 real sec [M::mem_process_seqs] Processed 701836 reads in 117.101 CPU sec, 3.688 real sec [M::process] read 701902 sequences (100000073 bp) in 2.144 CPU sec, 2.194 real sec... sorting block 132, #reads = 1086526 mem = 536879546 peak = 1158754304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 290239, 24, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 286, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1271) [M::mem_pestat] mean and std.dev: (313.58, 187.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 231, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (257.22, 129.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (285, 1184, 2636) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7338) [M::mem_pestat] mean and std.dev: (1496.57, 1573.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9689) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 305, 622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1426) [M::mem_pestat] mean and std.dev: (379.45, 239.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1828) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700652 sequences (100000116 bp) in 2.962 CPU sec, 3.100 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 290439, 14, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 321, 663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1547) [M::mem_pestat] mean and std.dev: (423.23, 294.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1989) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 231, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (258.49, 131.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (551, 927, 1691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3971) [M::mem_pestat] mean and std.dev: (1025.07, 614.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5111) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 294, 741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1873) [M::mem_pestat] mean and std.dev: (426.58, 379.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2439) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702048 reads in 174.693 CPU sec, 5.553 real sec [M::process] read 700122 sequences (100000256 bp) in 1.907 CPU sec, 1.922 real sec... [M::mem_process_seqs] Processed 701902 reads in 115.377 CPU sec, 3.636 real sec sorting block 133, #reads = 1086976 mem = 536879119 peak = 1158754304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 289900, 16, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (238, 372, 634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1426) [M::mem_pestat] mean and std.dev: (376.59, 253.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1822) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 237, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (263.94, 134.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (497, 723, 3970) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10916) [M::mem_pestat] mean and std.dev: (1923.25, 2426.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14389) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 368, 752) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1904) [M::mem_pestat] mean and std.dev: (509.70, 461.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2480) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699932 sequences (100000195 bp) in 3.187 CPU sec, 3.358 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 289617, 17, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 236, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (242.61, 145.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1064) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 237, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (264.65, 134.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (321, 558, 931) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2151) [M::mem_pestat] mean and std.dev: (563.80, 346.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2761) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 298, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048) [M::mem_pestat] mean and std.dev: (304.03, 136.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1322) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700652 reads in 175.236 CPU sec, 5.568 real sec sorting block 134, #reads = 1087119 mem = 536878074 peak = 1158754304 [M::process] read 699656 sequences (100000077 bp) in 2.280 CPU sec, 2.336 real sec... [M::mem_process_seqs] Processed 700122 reads in 116.900 CPU sec, 3.680 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 289760, 17, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 287, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (320.77, 169.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 239, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (265.74, 135.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (539, 1232, 2426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6200) [M::mem_pestat] mean and std.dev: (1380.35, 1168.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8087) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 240, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (262.74, 130.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1007) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 135, #reads = 1086063 mem = 536881892 peak = 1161523200 [M::process] read 699286 sequences (100000080 bp) in 3.203 CPU sec, 3.357 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 289970, 21, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 301, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050) [M::mem_pestat] mean and std.dev: (314.07, 153.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1337) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 239, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (265.94, 134.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (200, 721, 721) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1763) [M::mem_pestat] mean and std.dev: (560.21, 352.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2284) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 328, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (341.25, 192.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1170) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699932 reads in 175.521 CPU sec, 5.585 real sec [M::mem_process_seqs] Processed 699656 reads in 115.704 CPU sec, 3.638 real sec [M::process] read 698846 sequences (100000101 bp) in 2.120 CPU sec, 2.167 real sec... sorting block 136, #reads = 1086608 mem = 536884476 peak = 1161523200 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 288888, 31, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 339, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1049) [M::mem_pestat] mean and std.dev: (332.83, 204.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1342) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 240, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (267.14, 135.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (423, 963, 2130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5544) [M::mem_pestat] mean and std.dev: (1492.68, 1450.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7296) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 286, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1070) [M::mem_pestat] mean and std.dev: (283.08, 154.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1371) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700174 sequences (100000285 bp) in 2.403 CPU sec, 2.526 real sec... [M::process] read 699416 sequences (100000254 bp) in 1.520 CPU sec, 1.527 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 289315, 26, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (229, 339, 569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1249) [M::mem_pestat] mean and std.dev: (342.16, 175.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 242, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (268.86, 136.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (661, 1006, 2072) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4894) [M::mem_pestat] mean and std.dev: (945.62, 760.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6305) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 325, 613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1463) [M::mem_pestat] mean and std.dev: (367.24, 245.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1888) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699286 reads in 176.178 CPU sec, 5.632 real sec [M::mem_process_seqs] Processed 698846 reads in 118.075 CPU sec, 3.778 real sec sorting block 137, #reads = 1084404 mem = 536877186 peak = 1161523200 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 289909, 16, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 233, 557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1345) [M::mem_pestat] mean and std.dev: (319.16, 257.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1739) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 235, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (260.90, 131.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (216, 470, 1150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3018) [M::mem_pestat] mean and std.dev: (570.27, 520.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3952) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 311, 573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1353) [M::mem_pestat] mean and std.dev: (310.81, 176.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1743) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699844 sequences (100000289 bp) in 2.528 CPU sec, 2.687 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 289108, 12, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 206, 566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1406) [M::mem_pestat] mean and std.dev: (363.65, 322.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1826) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 238, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (265.32, 135.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (527, 987, 1210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2576) [M::mem_pestat] mean and std.dev: (865.91, 448.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3259) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 294, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1178) [M::mem_pestat] mean and std.dev: (284.72, 149.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1517) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 710582 sequences (100000105 bp) in 1.996 CPU sec, 1.997 real sec... [M::mem_process_seqs] Processed 700174 reads in 184.140 CPU sec, 5.866 real sec sorting block 138, #reads = 1083817 mem = 536878727 peak = 1161523200 [M::mem_process_seqs] Processed 699416 reads in 123.271 CPU sec, 3.908 real sec sorting block 139, #reads = 1081699 mem = 536883533 peak = 1161523200 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 288686, 24, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 259, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (292.47, 189.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1164) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 236, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (263.81, 134.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (601, 785, 1217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2449) [M::mem_pestat] mean and std.dev: (708.15, 455.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3065) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 213, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (232.55, 101.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 710366 sequences (100000122 bp) in 2.666 CPU sec, 2.788 real sec... [M::process] read 709566 sequences (100000093 bp) in 1.719 CPU sec, 1.723 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 292275, 12, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 246, 640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1602) [M::mem_pestat] mean and std.dev: (341.73, 337.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2083) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 212, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (237.26, 118.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (245, 1323, 1962) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5396) [M::mem_pestat] mean and std.dev: (1195.00, 1169.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7113) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 280, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1178) [M::mem_pestat] mean and std.dev: (320.71, 224.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1510) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699844 reads in 202.271 CPU sec, 6.726 real sec [M::mem_process_seqs] Processed 710582 reads in 126.373 CPU sec, 4.016 real sec sorting block 140, #reads = 1075456 mem = 536878839 peak = 1161523200 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 293188, 17, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 366, 674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1686) [M::mem_pestat] mean and std.dev: (441.67, 386.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2192) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 212, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (238.03, 119.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (211, 265, 2900) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8278) [M::mem_pestat] mean and std.dev: (1473.88, 1825.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10967) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 234, 827) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2149) [M::mem_pestat] mean and std.dev: (347.68, 392.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2810) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 710564 sequences (100000026 bp) in 2.557 CPU sec, 2.660 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 293210, 16, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 209, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1324) [M::mem_pestat] mean and std.dev: (310.51, 272.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 214, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (239.22, 119.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (430, 1135, 1518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3694) [M::mem_pestat] mean and std.dev: (760.00, 519.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4782) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 260, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1156) [M::mem_pestat] mean and std.dev: (286.02, 182.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1475) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 708020 sequences (100000043 bp) in 1.990 CPU sec, 1.991 real sec... [M::mem_process_seqs] Processed 710366 reads in 172.480 CPU sec, 5.481 real sec [M::mem_process_seqs] Processed 709566 reads in 114.592 CPU sec, 3.629 real sec sorting block 141, #reads = 1086720 mem = 536890702 peak = 1161523200 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 293529, 20, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 232, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (289.70, 186.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1163) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 211, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (237.08, 118.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (512, 631, 2576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6704) [M::mem_pestat] mean and std.dev: (1543.50, 1691.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8768) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 229, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810) [M::mem_pestat] mean and std.dev: (250.00, 132.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1025) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 709110 sequences (100000261 bp) in 2.714 CPU sec, 2.854 real sec... [M::process] read 708138 sequences (100000093 bp) in 1.802 CPU sec, 1.802 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 292507, 13, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 259, 732) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1812) [M::mem_pestat] mean and std.dev: (370.76, 290.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2352) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 217, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (242.47, 121.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (889, 1647, 1661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3205) [M::mem_pestat] mean and std.dev: (1215.67, 695.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3996) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 298, 1088) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2912) [M::mem_pestat] mean and std.dev: (424.88, 418.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3824) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 710564 reads in 194.396 CPU sec, 6.183 real sec sorting block 142, #reads = 1085581 mem = 536877884 peak = 1161523200 [M::mem_process_seqs] Processed 708020 reads in 127.761 CPU sec, 4.065 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 292882, 25, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 199, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1343) [M::mem_pestat] mean and std.dev: (322.38, 303.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 214, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (238.85, 119.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (405, 928, 6752) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 19446) [M::mem_pestat] mean and std.dev: (2689.60, 3416.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 25793) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 255, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (265.51, 120.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 143, #reads = 1083852 mem = 536880724 peak = 1161523200 [M::process] read 706552 sequences (100000019 bp) in 3.144 CPU sec, 3.271 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 292160, 16, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 283, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (278.74, 160.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 216, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (242.18, 121.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (256, 1240, 3050) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8638) [M::mem_pestat] mean and std.dev: (1677.31, 1678.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11432) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 276, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1155) [M::mem_pestat] mean and std.dev: (316.85, 196.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1477) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 705684 sequences (100000011 bp) in 2.231 CPU sec, 2.233 real sec... [M::mem_process_seqs] Processed 709110 reads in 201.638 CPU sec, 6.678 real sec [M::mem_process_seqs] Processed 708138 reads in 129.272 CPU sec, 4.076 real sec sorting block 144, #reads = 1085481 mem = 536880474 peak = 1161523200 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 291761, 15, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 424, 777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1917) [M::mem_pestat] mean and std.dev: (491.27, 374.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 220, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (246.84, 124.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (298, 298, 1464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3796) [M::mem_pestat] mean and std.dev: (745.20, 860.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4962) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 281, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960) [M::mem_pestat] mean and std.dev: (310.24, 195.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1217) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704250 sequences (100000109 bp) in 3.324 CPU sec, 3.493 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 292043, 25, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 228, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (222.82, 83.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1123) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 223, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (249.00, 125.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (431, 704, 1869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4745) [M::mem_pestat] mean and std.dev: (1173.50, 1128.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6183) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 305, 632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1546) [M::mem_pestat] mean and std.dev: (354.49, 272.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2003) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 706552 reads in 188.910 CPU sec, 6.007 real sec [M::process] read 703936 sequences (100000220 bp) in 2.325 CPU sec, 2.350 real sec... [M::mem_process_seqs] Processed 705684 reads in 130.774 CPU sec, 4.142 real sec sorting block 145, #reads = 1082731 mem = 536881652 peak = 1161523200 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 291141, 15, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 220, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (231.68, 124.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 227, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (253.25, 127.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (500, 802, 906) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1718) [M::mem_pestat] mean and std.dev: (567.08, 275.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2124) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 189, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (221.68, 111.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 994) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703588 sequences (100000232 bp) in 3.179 CPU sec, 3.341 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 291204, 20, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 374, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (345.54, 150.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1155) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 227, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (254.56, 129.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (242, 690, 1419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3773) [M::mem_pestat] mean and std.dev: (769.00, 755.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4950) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 305, 573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1295) [M::mem_pestat] mean and std.dev: (327.10, 201.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1656) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703654 sequences (100000154 bp) in 1.652 CPU sec, 1.653 real sec... [M::mem_process_seqs] Processed 704250 reads in 180.259 CPU sec, 5.735 real sec sorting block 146, #reads = 1086185 mem = 536880048 peak = 1161523200 [M::mem_process_seqs] Processed 703936 reads in 119.239 CPU sec, 3.768 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 291233, 33, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 304, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1049) [M::mem_pestat] mean and std.dev: (331.51, 216.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1340) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 228, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (255.53, 129.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (358, 629, 1372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3400) [M::mem_pestat] mean and std.dev: (896.00, 764.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4414) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 269, 678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1684) [M::mem_pestat] mean and std.dev: (353.50, 283.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2187) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 147, #reads = 1085995 mem = 536880611 peak = 1161523200 [M::process] read 702954 sequences (100000232 bp) in 3.115 CPU sec, 3.254 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 291388, 26, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 212, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088) [M::mem_pestat] mean and std.dev: (242.92, 165.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 228, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (255.93, 130.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (323, 667, 1024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2426) [M::mem_pestat] mean and std.dev: (675.61, 416.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3127) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 263, 669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1593) [M::mem_pestat] mean and std.dev: (340.59, 250.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2055) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702738 sequences (100000055 bp) in 1.460 CPU sec, 1.458 real sec... [M::mem_process_seqs] Processed 703588 reads in 168.446 CPU sec, 5.350 real sec [M::mem_process_seqs] Processed 703654 reads in 108.378 CPU sec, 3.439 real sec sorting block 148, #reads = 1087126 mem = 536879343 peak = 1161523200 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 290795, 8, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (239, 403, 1130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2912) [M::mem_pestat] mean and std.dev: (566.84, 519.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3803) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 230, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (257.04, 130.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 259, 673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1667) [M::mem_pestat] mean and std.dev: (408.38, 370.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2164) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 702726 sequences (100000113 bp) in 2.595 CPU sec, 2.713 real sec... [M::process] read 702062 sequences (100000005 bp) in 1.889 CPU sec, 1.890 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 290585, 22, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 352, 566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1326) [M::mem_pestat] mean and std.dev: (359.70, 247.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 230, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (258.36, 131.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (220, 2471, 2471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6973) [M::mem_pestat] mean and std.dev: (1504.27, 1300.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9224) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 324, 1496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4056) [M::mem_pestat] mean and std.dev: (675.02, 866.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5336) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702954 reads in 176.939 CPU sec, 5.915 real sec [M::mem_process_seqs] Processed 702738 reads in 112.708 CPU sec, 3.571 real sec sorting block 149, #reads = 1086801 mem = 536881627 peak = 1161523200 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 290696, 12, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 258, 715) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1823) [M::mem_pestat] mean and std.dev: (367.20, 369.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2377) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 230, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (258.24, 131.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (78, 446, 1136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3252) [M::mem_pestat] mean and std.dev: (705.00, 747.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4310) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 331, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1130) [M::mem_pestat] mean and std.dev: (347.03, 192.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1435) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701526 sequences (100000160 bp) in 3.127 CPU sec, 3.267 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 290981, 21, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 254, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1146) [M::mem_pestat] mean and std.dev: (296.46, 170.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 232, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (260.28, 133.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (367, 576, 3985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11221) [M::mem_pestat] mean and std.dev: (1795.71, 2024.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14839) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 307, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1124) [M::mem_pestat] mean and std.dev: (321.11, 170.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1423) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702726 reads in 181.167 CPU sec, 5.759 real sec [M::process] read 701074 sequences (100000161 bp) in 2.339 CPU sec, 2.372 real sec... [M::mem_process_seqs] Processed 702062 reads in 121.354 CPU sec, 3.823 real sec sorting block 150, #reads = 1086692 mem = 536878236 peak = 1161523200 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 290439, 11, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 436, 863) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2193) [M::mem_pestat] mean and std.dev: (541.11, 402.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2858) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 234, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (262.27, 134.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (504, 696, 2201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5595) [M::mem_pestat] mean and std.dev: (870.10, 817.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7292) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 252, 571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1271) [M::mem_pestat] mean and std.dev: (323.86, 237.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1621) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700698 sequences (100000003 bp) in 2.870 CPU sec, 3.031 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 289890, 19, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 273, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1127) [M::mem_pestat] mean and std.dev: (310.41, 190.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 236, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (263.12, 133.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (345, 945, 1200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2910) [M::mem_pestat] mean and std.dev: (730.50, 579.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3765) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 254, 539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1299) [M::mem_pestat] mean and std.dev: (316.81, 261.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1679) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700430 sequences (100000213 bp) in 1.950 CPU sec, 1.952 real sec... [M::mem_process_seqs] Processed 701526 reads in 181.084 CPU sec, 5.764 real sec sorting block 151, #reads = 1086123 mem = 536877043 peak = 1161523200 [M::mem_process_seqs] Processed 701074 reads in 119.982 CPU sec, 4.060 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 289776, 16, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 277, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951) [M::mem_pestat] mean and std.dev: (283.65, 150.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1210) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 236, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (262.98, 132.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (729, 2645, 6534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18144) [M::mem_pestat] mean and std.dev: (3281.19, 2705.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 23949) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 236, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050) [M::mem_pestat] mean and std.dev: (283.71, 183.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1338) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 152, #reads = 1085781 mem = 536879313 peak = 1161523200 [M::process] read 699772 sequences (100000218 bp) in 3.137 CPU sec, 3.296 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 290644, 20, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 298, 858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2220) [M::mem_pestat] mean and std.dev: (454.39, 458.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2901) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 238, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (264.66, 134.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (273, 957, 5531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16047) [M::mem_pestat] mean and std.dev: (2493.50, 2685.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21305) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 311, 1067) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2801) [M::mem_pestat] mean and std.dev: (576.76, 533.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3668) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700698 reads in 177.883 CPU sec, 5.925 real sec [M::process] read 699226 sequences (100000225 bp) in 2.210 CPU sec, 2.253 real sec... [M::mem_process_seqs] Processed 700430 reads in 116.318 CPU sec, 3.664 real sec sorting block 153, #reads = 1085201 mem = 536881949 peak = 1161523200 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 289408, 24, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 301, 583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1389) [M::mem_pestat] mean and std.dev: (303.28, 179.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1792) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 240, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (267.84, 136.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1134, 2503, 2982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6678) [M::mem_pestat] mean and std.dev: (1993.29, 1383.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8526) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 331, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1120) [M::mem_pestat] mean and std.dev: (317.47, 201.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1433) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698656 sequences (100000213 bp) in 2.390 CPU sec, 2.532 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 289724, 20, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 268, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1156) [M::mem_pestat] mean and std.dev: (290.60, 165.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 243, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (269.67, 137.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (465, 1016, 1437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3381) [M::mem_pestat] mean and std.dev: (854.28, 546.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4353) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 336, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093) [M::mem_pestat] mean and std.dev: (309.93, 187.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1398) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699284 sequences (100000003 bp) in 1.470 CPU sec, 1.471 real sec... [M::mem_process_seqs] Processed 699772 reads in 164.491 CPU sec, 5.241 real sec [M::mem_process_seqs] Processed 699226 reads in 107.977 CPU sec, 3.415 real sec sorting block 154, #reads = 1086684 mem = 536879193 peak = 1161523200 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 288967, 18, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 202, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (219.69, 104.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 243, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (269.82, 137.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (789, 1415, 3414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8664) [M::mem_pestat] mean and std.dev: (1908.61, 1290.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11289) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 401, 695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1703) [M::mem_pestat] mean and std.dev: (411.19, 287.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2207) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698916 sequences (100000138 bp) in 2.956 CPU sec, 3.112 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 289440, 21, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 317, 602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1406) [M::mem_pestat] mean and std.dev: (353.25, 240.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 242, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (269.21, 136.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (217, 363, 1545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4201) [M::mem_pestat] mean and std.dev: (942.00, 997.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5529) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 302, 579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1293) [M::mem_pestat] mean and std.dev: (380.53, 276.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1650) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698656 reads in 166.275 CPU sec, 5.285 real sec [M::process] read 697360 sequences (100000280 bp) in 2.064 CPU sec, 2.093 real sec... sorting block 155, #reads = 1086294 mem = 536880565 peak = 1161523200 [M::mem_process_seqs] Processed 699284 reads in 116.417 CPU sec, 3.684 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 288956, 20, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 367, 909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2325) [M::mem_pestat] mean and std.dev: (493.83, 545.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3033) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 241, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (266.95, 134.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (579, 2246, 4144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11274) [M::mem_pestat] mean and std.dev: (2608.65, 2446.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14839) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 362, 826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2110) [M::mem_pestat] mean and std.dev: (488.44, 429.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2752) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 156, #reads = 1085601 mem = 536879768 peak = 1161523200 [M::process] read 697390 sequences (100000212 bp) in 2.589 CPU sec, 2.727 real sec... [M::process] read 207778 sequences (29664544 bp) in 0.780 CPU sec, 0.786 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 288490, 25, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 305, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1258) [M::mem_pestat] mean and std.dev: (368.82, 299.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (270.99, 135.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (183, 558, 2449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6981) [M::mem_pestat] mean and std.dev: (1610.36, 1882.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9247) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 295, 644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1504) [M::mem_pestat] mean and std.dev: (327.84, 189.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1934) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698916 reads in 166.102 CPU sec, 5.307 real sec [M::mem_process_seqs] Processed 697360 reads in 107.613 CPU sec, 3.424 real sec sorting block 157, #reads = 1084047 mem = 536879904 peak = 1161523200 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 288508, 20, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 245, 714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1830) [M::mem_pestat] mean and std.dev: (328.16, 254.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 245, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (269.89, 134.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (432, 774, 1194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2718) [M::mem_pestat] mean and std.dev: (713.22, 401.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3480) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 246, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (236.32, 97.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 85912, 6, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 158, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (189.31, 65.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 238, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (262.77, 131.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 417, 661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1577) [M::mem_pestat] mean and std.dev: (320.53, 177.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2035) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697390 reads in 116.294 CPU sec, 3.774 real sec [M::mem_process_seqs] Processed 207778 reads in 48.417 CPU sec, 1.563 real sec sorting block 158, #reads = 1084306 mem = 536880762 peak = 1161523200 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 171848848 overall: 70072819712 mem 24044.186 user 640.245 sys 815.700 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_86S-T1-TDNA-1_A23MHWWLT4_1.L006\tPU:L006\tSM:1136_86S-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_86S-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1136_86S-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 1136_86S-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 815.739 sec; CPU: 24684.439 sec sorting block 159, #reads = 257715 mem = 128634397 peak = 1161523200 uniq header count 1 merging 160 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 9415776996 output reads: 173023257 bam_mem_sort: 160 calls 87.593 user 1.650 sys 89.991 real bam_write: 160 calls 55.962 user 3.437 sys 70.282 real execute: 1 calls 35.360 user 1.569 sys 866.792 real merge_files: 1 calls 35.327 user 1.561 sys 50.928 real parse_chunk: 2548 calls 109.912 user 28.219 sys 145.493 real read_chunk: 6886 calls 52.920 user 47.046 sys 760.611 real sort_block: 1 calls 35.801 user 46.380 sys 736.414 real write_chunk: 4336 calls 34.939 user 1.225 sys 49.834 real overall: 3499302912 mem 2070.233 user 101.247 sys 866.814 real ls: cannot access '*.cram': No such file or directory