cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_3U6-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 No more license available for sentieon:klib No more license available for sentieon:klib [M::bwa_idx_load_from_disk] read 3171 ALT contigs No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib Received only 16 licenses out of 31 requested for sentieon:klib Received only 16 licenses out of 31 requested for sentieon:klib [M::main_mem] bwa index loaded in 1658.671 CPU sec, 949.800 real sec [M::process] read 686960 sequences (100000236 bp) in 0.962 CPU sec, 0.966 real sec... [M::process] read 686242 sequences (100000258 bp) in 1.040 CPU sec, 1.041 real sec... [M::process] read 684872 sequences (100000143 bp) in 1.078 CPU sec, 1.110 real sec... [M::process] read 683706 sequences (100000266 bp) in 1.081 CPU sec, 1.120 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 284159, 5, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 350, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1106) [M::mem_pestat] mean and std.dev: (377.00, 190.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 301, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (321.27, 160.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 273, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (236.13, 69.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 283653, 4, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 272, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (80, 445) [M::mem_pestat] mean and std.dev: (252.82, 67.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 314, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932) [M::mem_pestat] mean and std.dev: (332.09, 163.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1177) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 233, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (236.35, 102.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 284139, 7, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 270, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (6, 516) [M::mem_pestat] mean and std.dev: (219.86, 74.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 306, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (325.32, 161.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1159) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 327, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (269.29, 96.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1168) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686960 reads in 191.278 CPU sec, 10.674 real sec [M::mem_process_seqs] Processed 684872 reads in 139.189 CPU sec, 7.206 real sec [M::mem_process_seqs] Processed 686242 reads in 151.404 CPU sec, 7.626 real sec sorting block 0, #reads = 1082081 mem = 536879610 peak = 599572480 [M::process] read 683272 sequences (100000008 bp) in 1.601 CPU sec, 1.861 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282791, 7, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 409, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1190) [M::mem_pestat] mean and std.dev: (380.79, 164.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 323, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960) [M::mem_pestat] mean and std.dev: (340.04, 167.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1213) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (251, 297, 607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1319) [M::mem_pestat] mean and std.dev: (405.57, 270.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1675) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683260 sequences (100000062 bp) in 1.259 CPU sec, 1.286 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282904, 6, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 293, 893) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2269) [M::mem_pestat] mean and std.dev: (550.88, 529.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2957) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 324, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961) [M::mem_pestat] mean and std.dev: (341.09, 167.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1214) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 285, 1182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3198) [M::mem_pestat] mean and std.dev: (590.33, 580.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4206) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683444 sequences (100000031 bp) in 1.110 CPU sec, 1.110 real sec... [M::mem_process_seqs] Processed 683706 reads in 136.133 CPU sec, 4.324 real sec sorting block 1, #reads = 1081012 mem = 536879741 peak = 928002048 [M::mem_process_seqs] Processed 683272 reads in 85.476 CPU sec, 2.711 real sec sorting block 2, #reads = 1080829 mem = 536879610 peak = 973672448 [M::process] read 682952 sequences (100000067 bp) in 1.560 CPU sec, 1.659 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282777, 7, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (270, 324, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (118, 498) [M::mem_pestat] mean and std.dev: (285.46, 71.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 322, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952) [M::mem_pestat] mean and std.dev: (339.29, 166.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1202) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 238, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (256.80, 143.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682260 sequences (100000189 bp) in 1.224 CPU sec, 1.223 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282765, 3, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 305, 826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2104) [M::mem_pestat] mean and std.dev: (466.48, 396.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 319, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942) [M::mem_pestat] mean and std.dev: (337.04, 165.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1189) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 384, 881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2189) [M::mem_pestat] mean and std.dev: (493.91, 355.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2843) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 282449, 8, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 221, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (186.75, 47.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 322, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952) [M::mem_pestat] mean and std.dev: (339.40, 166.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1202) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 274, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (284.25, 110.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683260 reads in 196.415 CPU sec, 6.306 real sec [M::mem_process_seqs] Processed 683444 reads in 145.458 CPU sec, 4.685 real sec [M::mem_process_seqs] Processed 682952 reads in 157.553 CPU sec, 5.100 real sec sorting block 3, #reads = 1080264 mem = 536882616 peak = 973672448 [M::process] read 681824 sequences (100000300 bp) in 1.883 CPU sec, 2.014 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282090, 7, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 292, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011) [M::mem_pestat] mean and std.dev: (316.06, 151.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1278) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 326, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949) [M::mem_pestat] mean and std.dev: (341.82, 165.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1197) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (320, 371, 581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1103) [M::mem_pestat] mean and std.dev: (362.87, 136.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1364) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 4, #reads = 1080332 mem = 536877211 peak = 991092736 [M::process] read 681076 sequences (100000207 bp) in 1.403 CPU sec, 1.447 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282200, 3, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 298, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (315.46, 184.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 329, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (343.95, 165.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1192) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 388, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962) [M::mem_pestat] mean and std.dev: (324.26, 171.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1223) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682260 reads in 138.904 CPU sec, 4.428 real sec [M::mem_process_seqs] Processed 681824 reads in 86.906 CPU sec, 2.740 real sec [M::process] read 683804 sequences (100000205 bp) in 1.494 CPU sec, 1.549 real sec... sorting block 5, #reads = 1078688 mem = 536881487 peak = 991092736 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 281875, 2, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 413, 728) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1854) [M::mem_pestat] mean and std.dev: (450.14, 311.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 334, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965) [M::mem_pestat] mean and std.dev: (349.40, 167.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1216) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 589, 629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1551) [M::mem_pestat] mean and std.dev: (543.53, 410.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2187) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686532 sequences (100000076 bp) in 1.865 CPU sec, 1.997 real sec... [M::process] read 685616 sequences (100000118 bp) in 1.230 CPU sec, 1.227 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 282941, 1, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 234, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (227.67, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 321, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951) [M::mem_pestat] mean and std.dev: (338.90, 167.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1201) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 260, 570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1376) [M::mem_pestat] mean and std.dev: (316.25, 197.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1779) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681076 reads in 134.920 CPU sec, 4.298 real sec [M::mem_process_seqs] Processed 683804 reads in 85.245 CPU sec, 2.704 real sec sorting block 6, #reads = 1078957 mem = 536878625 peak = 991092736 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283788, 9, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 178, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953) [M::mem_pestat] mean and std.dev: (260.72, 159.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1221) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 307, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (327.06, 164.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1175) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 432, 574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1378) [M::mem_pestat] mean and std.dev: (340.85, 230.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1780) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684506 sequences (100000093 bp) in 1.701 CPU sec, 1.816 real sec... [M::process] read 683744 sequences (100000062 bp) in 1.249 CPU sec, 1.252 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 283402, 6, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 352, 718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1694) [M::mem_pestat] mean and std.dev: (436.45, 252.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2182) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 313, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (330.59, 164.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1182) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (274, 350, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (346.38, 130.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686532 reads in 135.731 CPU sec, 4.328 real sec [M::mem_process_seqs] Processed 685616 reads in 84.427 CPU sec, 2.686 real sec sorting block 7, #reads = 1081747 mem = 536877925 peak = 991092736 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282713, 5, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 236, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (290.21, 126.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 321, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954) [M::mem_pestat] mean and std.dev: (337.10, 166.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1206) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (321, 349, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (342.71, 133.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683632 sequences (100000283 bp) in 1.995 CPU sec, 2.129 real sec... [M::process] read 683550 sequences (100000020 bp) in 1.260 CPU sec, 1.264 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 283116, 4, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 238, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (276.96, 124.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 326, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967) [M::mem_pestat] mean and std.dev: (341.96, 168.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1222) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (129, 205, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1137) [M::mem_pestat] mean and std.dev: (245.00, 149.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1473) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684506 reads in 134.669 CPU sec, 4.283 real sec sorting block 8, #reads = 1082680 mem = 536879958 peak = 991092736 [M::mem_process_seqs] Processed 683744 reads in 84.072 CPU sec, 2.669 real sec sorting block 9, #reads = 1081312 mem = 536879504 peak = 1009647616 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 282882, 5, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 314, 712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1802) [M::mem_pestat] mean and std.dev: (399.68, 309.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2347) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 326, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973) [M::mem_pestat] mean and std.dev: (342.69, 169.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1230) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 334, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1095) [M::mem_pestat] mean and std.dev: (338.56, 213.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1406) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683612 sequences (100000082 bp) in 1.926 CPU sec, 2.079 real sec... [M::process] read 683248 sequences (100000187 bp) in 1.320 CPU sec, 1.317 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 283005, 5, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (246, 473, 604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1320) [M::mem_pestat] mean and std.dev: (511.26, 303.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 322, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957) [M::mem_pestat] mean and std.dev: (339.95, 167.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1209) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 261, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (254.45, 95.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683632 reads in 136.974 CPU sec, 4.361 real sec [M::mem_process_seqs] Processed 683550 reads in 85.863 CPU sec, 2.722 real sec sorting block 10, #reads = 1080820 mem = 536880020 peak = 1009647616 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282915, 6, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 226, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (80, 350) [M::mem_pestat] mean and std.dev: (193.86, 50.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 404) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 316, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (334.30, 163.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1179) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 262, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1122) [M::mem_pestat] mean and std.dev: (337.30, 213.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1422) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682614 sequences (100000025 bp) in 2.085 CPU sec, 2.222 real sec... [M::process] read 681986 sequences (100000264 bp) in 1.310 CPU sec, 1.305 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282884, 6, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 319, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1089) [M::mem_pestat] mean and std.dev: (312.67, 140.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 321, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (337.35, 164.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1178) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (247, 280, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (307.45, 142.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683612 reads in 136.457 CPU sec, 4.337 real sec [M::mem_process_seqs] Processed 683248 reads in 85.307 CPU sec, 2.707 real sec sorting block 11, #reads = 1080558 mem = 536878530 peak = 1009647616 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282868, 5, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 262, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (278.50, 131.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 324, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948) [M::mem_pestat] mean and std.dev: (341.00, 165.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1196) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 370, 1218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3190) [M::mem_pestat] mean and std.dev: (757.00, 674.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4176) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681784 sequences (100000074 bp) in 1.978 CPU sec, 2.139 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 282202, 9, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 222, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (250.58, 92.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 328, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959) [M::mem_pestat] mean and std.dev: (344.70, 167.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1210) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 408, 679) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1679) [M::mem_pestat] mean and std.dev: (408.86, 309.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2179) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687418 sequences (100000162 bp) in 1.330 CPU sec, 1.330 real sec... [M::mem_process_seqs] Processed 682614 reads in 134.390 CPU sec, 4.280 real sec [M::mem_process_seqs] Processed 681986 reads in 84.526 CPU sec, 2.677 real sec sorting block 12, #reads = 1080019 mem = 536878254 peak = 1009647616 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282760, 16, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 361, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046) [M::mem_pestat] mean and std.dev: (354.94, 254.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 333, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974) [M::mem_pestat] mean and std.dev: (349.45, 170.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1229) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (62, 278, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1382) [M::mem_pestat] mean and std.dev: (269.53, 234.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1822) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (244, 305, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (279.60, 127.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687242 sequences (100000265 bp) in 2.108 CPU sec, 2.226 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 284307, 4, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (359, 415, 614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1124) [M::mem_pestat] mean and std.dev: (424.89, 200.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 303, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (322.27, 161.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (130, 261, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (243.00, 114.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685774 sequences (100000272 bp) in 1.350 CPU sec, 1.347 real sec... [M::mem_process_seqs] Processed 681784 reads in 136.234 CPU sec, 4.332 real sec sorting block 13, #reads = 1079295 mem = 536881799 peak = 1009647616 [M::mem_process_seqs] Processed 687418 reads in 86.008 CPU sec, 2.723 real sec sorting block 14, #reads = 1080297 mem = 536891435 peak = 1064579072 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 284351, 8, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 222, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (81, 326) [M::mem_pestat] mean and std.dev: (184.55, 39.09) [M::mem_pestat] low and high boundaries for proper pairs: (28, 375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 303, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (320.93, 158.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::process] read 684638 sequences (100000265 bp) in 1.941 CPU sec, 2.079 real sec... [M::process] read 683762 sequences (100000059 bp) in 1.360 CPU sec, 1.358 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283838, 5, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 376, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (341.56, 213.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1194) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 311, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (327.11, 159.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1158) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 297, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (265.74, 106.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687242 reads in 139.059 CPU sec, 4.428 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283002, 3, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 411, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (354.19, 170.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1165) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 318, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (332.42, 161.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 325, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1242) [M::mem_pestat] mean and std.dev: (350.39, 182.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1594) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683424 sequences (100000287 bp) in 1.736 CPU sec, 1.740 real sec... [M::mem_process_seqs] Processed 684638 reads in 75.756 CPU sec, 2.397 real sec sorting block 15, #reads = 1082209 mem = 536880634 peak = 1064579072 [M::mem_process_seqs] Processed 685774 reads in 190.345 CPU sec, 6.051 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282566, 4, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (261, 299, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (59, 564) [M::mem_pestat] mean and std.dev: (290.25, 67.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 320, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (335.20, 162.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1184) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 291, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (277.61, 87.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1138) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 16, #reads = 1081875 mem = 536880086 peak = 1064579072 [M::process] read 683322 sequences (100000179 bp) in 1.956 CPU sec, 2.120 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283379, 5, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 243, 638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1550) [M::mem_pestat] mean and std.dev: (348.88, 231.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2006) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 323, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943) [M::mem_pestat] mean and std.dev: (338.14, 163.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1190) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 302, 589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1371) [M::mem_pestat] mean and std.dev: (340.72, 177.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1762) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683036 sequences (100000058 bp) in 1.330 CPU sec, 1.325 real sec... [M::mem_process_seqs] Processed 683762 reads in 151.113 CPU sec, 4.803 real sec [M::mem_process_seqs] Processed 683424 reads in 87.442 CPU sec, 2.762 real sec sorting block 17, #reads = 1080426 mem = 536883678 peak = 1064579072 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282623, 1, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 470, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1229) [M::mem_pestat] mean and std.dev: (356.63, 204.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 319, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936) [M::mem_pestat] mean and std.dev: (335.88, 163.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1181) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 304, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966) [M::mem_pestat] mean and std.dev: (345.38, 168.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1214) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683014 sequences (100000134 bp) in 2.362 CPU sec, 2.542 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282570, 5, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 549, 882) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2324) [M::mem_pestat] mean and std.dev: (459.40, 369.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3045) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 320, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937) [M::mem_pestat] mean and std.dev: (336.68, 163.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1182) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 287, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (267.57, 109.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1169) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682600 sequences (100000215 bp) in 1.350 CPU sec, 1.348 real sec... [M::mem_process_seqs] Processed 683322 reads in 139.960 CPU sec, 4.445 real sec [M::mem_process_seqs] Processed 683036 reads in 88.530 CPU sec, 2.816 real sec sorting block 18, #reads = 1080275 mem = 536883462 peak = 1064579072 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282550, 7, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 242, 658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1562) [M::mem_pestat] mean and std.dev: (524.06, 499.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 321, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931) [M::mem_pestat] mean and std.dev: (336.27, 162.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 265, 548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1174) [M::mem_pestat] mean and std.dev: (334.18, 161.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1487) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682318 sequences (100000071 bp) in 1.844 CPU sec, 1.968 real sec... [M::process] read 685424 sequences (100000204 bp) in 1.360 CPU sec, 1.360 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282829, 7, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (306, 306, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (320.45, 122.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 325, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954) [M::mem_pestat] mean and std.dev: (341.52, 166.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1204) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 309, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (300.06, 140.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683014 reads in 139.200 CPU sec, 4.436 real sec sorting block 19, #reads = 1079019 mem = 536878211 peak = 1064579072 [M::mem_process_seqs] Processed 682600 reads in 87.174 CPU sec, 2.764 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 282640, 4, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 345, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1139) [M::mem_pestat] mean and std.dev: (291.37, 123.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 327, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963) [M::mem_pestat] mean and std.dev: (344.25, 169.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1216) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 332, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (282.61, 125.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686614 sequences (100000299 bp) in 2.213 CPU sec, 2.379 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 283551, 1, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 195, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (238.22, 101.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 311, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (329.58, 163.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 325, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1107) [M::mem_pestat] mean and std.dev: (360.13, 213.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1408) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685940 sequences (100000213 bp) in 1.350 CPU sec, 1.348 real sec... [M::mem_process_seqs] Processed 682318 reads in 137.694 CPU sec, 4.378 real sec sorting block 20, #reads = 1078893 mem = 536879372 peak = 1064579072 [M::mem_process_seqs] Processed 685424 reads in 87.781 CPU sec, 2.785 real sec sorting block 21, #reads = 1077631 mem = 536879746 peak = 1064579072 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 284322, 2, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (261, 547, 876) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2106) [M::mem_pestat] mean and std.dev: (532.69, 250.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 305, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (323.56, 160.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1151) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 243, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (268.93, 125.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684538 sequences (100000104 bp) in 1.962 CPU sec, 2.106 real sec... [M::process] read 684040 sequences (100000003 bp) in 1.340 CPU sec, 1.342 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 283223, 2, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 301, 1166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3136) [M::mem_pestat] mean and std.dev: (600.84, 698.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4121) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 310, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (327.31, 161.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1169) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 364, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052) [M::mem_pestat] mean and std.dev: (340.15, 174.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1338) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686614 reads in 138.236 CPU sec, 4.401 real sec [M::mem_process_seqs] Processed 685940 reads in 87.360 CPU sec, 2.774 real sec sorting block 22, #reads = 1083262 mem = 536881393 peak = 1064579072 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282591, 4, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 431, 860) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2160) [M::mem_pestat] mean and std.dev: (464.39, 322.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2810) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 318, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (333.93, 163.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1186) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (298, 405, 572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1120) [M::mem_pestat] mean and std.dev: (383.52, 133.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1394) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684054 sequences (100000104 bp) in 2.185 CPU sec, 2.333 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282036, 4, 23) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 320, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (334.70, 162.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1180) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 411, 741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1803) [M::mem_pestat] mean and std.dev: (519.74, 358.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2334) [M::mem_pestat] skip orientation RR [M::process] read 684678 sequences (100000131 bp) in 1.350 CPU sec, 1.351 real sec... [M::mem_process_seqs] Processed 684538 reads in 141.109 CPU sec, 4.494 real sec [M::mem_process_seqs] Processed 684040 reads in 88.992 CPU sec, 2.817 real sec sorting block 23, #reads = 1082108 mem = 536881564 peak = 1064579072 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282572, 4, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 313, 571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1417) [M::mem_pestat] mean and std.dev: (327.95, 186.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1840) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 321, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (336.12, 163.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1192) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (274, 396, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (365.23, 126.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1082) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684128 sequences (100000019 bp) in 1.983 CPU sec, 2.123 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283780, 5, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (256, 729, 877) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2119) [M::mem_pestat] mean and std.dev: (728.54, 508.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 310, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (329.00, 161.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1171) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 301, 1078) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2818) [M::mem_pestat] mean and std.dev: (494.05, 415.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3688) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683714 sequences (100000012 bp) in 1.350 CPU sec, 1.349 real sec... [M::mem_process_seqs] Processed 684054 reads in 142.116 CPU sec, 4.535 real sec sorting block 24, #reads = 1080384 mem = 536880307 peak = 1064579072 [M::mem_process_seqs] Processed 684678 reads in 88.781 CPU sec, 2.815 real sec sorting block 25, #reads = 1079701 mem = 536879087 peak = 1064579072 [M::process] read 683250 sequences (100000137 bp) in 2.254 CPU sec, 2.396 real sec... [M::process] read 682712 sequences (100000078 bp) in 1.350 CPU sec, 1.348 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282330, 5, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 277, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (240.88, 97.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 313, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (329.46, 158.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (262, 374, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (353.21, 163.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683714 reads in 77.725 CPU sec, 2.452 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282333, 5, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (229, 279, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (304.04, 139.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 312, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (328.64, 158.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 602, 872) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2218) [M::mem_pestat] mean and std.dev: (537.25, 366.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2891) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282551, 7, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 389, 832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2006) [M::mem_pestat] mean and std.dev: (516.26, 387.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 315, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (331.32, 158.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 279, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (286.57, 110.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686234 sequences (100000014 bp) in 1.687 CPU sec, 1.687 real sec... [M::mem_process_seqs] Processed 684128 reads in 219.711 CPU sec, 6.977 real sec [M::mem_process_seqs] Processed 683250 reads in 101.840 CPU sec, 3.250 real sec sorting block 26, #reads = 1078183 mem = 536879556 peak = 1064579072 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282753, 5, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 180, 716) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1872) [M::mem_pestat] mean and std.dev: (410.76, 347.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 322, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947) [M::mem_pestat] mean and std.dev: (339.46, 164.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1195) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 258, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (263.00, 106.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 27, #reads = 1078700 mem = 536881575 peak = 1064579072 [M::process] read 689540 sequences (100000068 bp) in 2.055 CPU sec, 2.216 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 284454, 2, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 226, 581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1417) [M::mem_pestat] mean and std.dev: (390.04, 334.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1835) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 305, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932) [M::mem_pestat] mean and std.dev: (327.43, 165.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1179) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 309, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985) [M::mem_pestat] mean and std.dev: (306.41, 148.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1245) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687076 sequences (100000246 bp) in 1.330 CPU sec, 1.331 real sec... [M::mem_process_seqs] Processed 682712 reads in 140.039 CPU sec, 4.467 real sec [M::mem_process_seqs] Processed 686234 reads in 89.316 CPU sec, 2.830 real sec sorting block 28, #reads = 1078486 mem = 536883925 peak = 1064579072 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 285507, 3, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 295, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1303) [M::mem_pestat] mean and std.dev: (287.62, 147.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 285, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (312.65, 161.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::process] read 686048 sequences (100000010 bp) in 2.241 CPU sec, 2.404 real sec... [M::process] read 684828 sequences (100000020 bp) in 1.370 CPU sec, 1.375 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 284783, 5, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 378, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (316.43, 108.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 303, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (326.82, 166.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1196) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 220, 585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1463) [M::mem_pestat] mean and std.dev: (268.38, 196.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1902) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689540 reads in 140.641 CPU sec, 4.467 real sec [M::mem_process_seqs] Processed 687076 reads in 86.809 CPU sec, 2.756 real sec sorting block 29, #reads = 1080792 mem = 536878121 peak = 1064579072 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 283835, 4, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 270, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (253.67, 131.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 311, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952) [M::mem_pestat] mean and std.dev: (331.45, 166.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1205) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 269, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957) [M::mem_pestat] mean and std.dev: (348.58, 236.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1293) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684282 sequences (100000129 bp) in 1.949 CPU sec, 2.077 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 283503, 6, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 413, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042) [M::mem_pestat] mean and std.dev: (376.47, 273.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 684162 sequences (100000140 bp) in 1.370 CPU sec, 1.373 real sec... [M::mem_pestat] (25, 50, 75) percentile: (196, 317, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958) [M::mem_pestat] mean and std.dev: (335.71, 166.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1212) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (270, 384, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (360.59, 178.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686048 reads in 142.466 CPU sec, 4.547 real sec [M::mem_process_seqs] Processed 684828 reads in 90.018 CPU sec, 2.861 real sec sorting block 30, #reads = 1080552 mem = 536879702 peak = 1064579072 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 283331, 3, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 354, 984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2626) [M::mem_pestat] mean and std.dev: (660.56, 640.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 319, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960) [M::mem_pestat] mean and std.dev: (336.75, 165.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1214) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 260, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945) [M::mem_pestat] mean and std.dev: (309.62, 145.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1195) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683912 sequences (100000169 bp) in 2.153 CPU sec, 2.276 real sec... [M::process] read 683652 sequences (100000017 bp) in 1.390 CPU sec, 1.389 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283201, 4, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 433, 768) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1862) [M::mem_pestat] mean and std.dev: (594.18, 467.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 319, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951) [M::mem_pestat] mean and std.dev: (336.20, 165.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1202) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 190, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984) [M::mem_pestat] mean and std.dev: (289.69, 172.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1255) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684282 reads in 143.842 CPU sec, 4.574 real sec sorting block 31, #reads = 1078367 mem = 536880322 peak = 1064579072 [M::mem_process_seqs] Processed 684162 reads in 89.911 CPU sec, 2.857 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 283291, 4, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 215, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (226.54, 88.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 317, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943) [M::mem_pestat] mean and std.dev: (335.18, 164.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1191) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (244, 374, 580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1252) [M::mem_pestat] mean and std.dev: (405.72, 195.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1588) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 32, #reads = 1078104 mem = 536879092 peak = 1092300800 [M::process] read 683270 sequences (100000289 bp) in 1.940 CPU sec, 2.055 real sec... [M::process] read 683074 sequences (100000215 bp) in 1.400 CPU sec, 1.398 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283067, 10, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 284, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1210) [M::mem_pestat] mean and std.dev: (294.40, 154.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 318, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (335.22, 163.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1195) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (267, 327, 1268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3270) [M::mem_pestat] mean and std.dev: (648.70, 548.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4271) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 361, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (299.53, 110.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683912 reads in 145.029 CPU sec, 4.627 real sec [M::mem_process_seqs] Processed 683652 reads in 91.552 CPU sec, 2.901 real sec sorting block 33, #reads = 1077274 mem = 536878896 peak = 1092300800 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 283457, 3, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 373, 737) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1849) [M::mem_pestat] mean and std.dev: (423.30, 275.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2405) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 320, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940) [M::mem_pestat] mean and std.dev: (337.09, 164.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1186) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 258, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (266.47, 77.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689964 sequences (100000244 bp) in 2.002 CPU sec, 2.124 real sec... [M::process] read 692726 sequences (100000060 bp) in 1.420 CPU sec, 1.424 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283145, 3, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (240, 320, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (333.54, 143.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 324, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959) [M::mem_pestat] mean and std.dev: (341.54, 167.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1211) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 398, 578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1286) [M::mem_pestat] mean and std.dev: (376.00, 208.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1640) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683270 reads in 143.327 CPU sec, 4.563 real sec [M::mem_process_seqs] Processed 683074 reads in 90.015 CPU sec, 2.854 real sec sorting block 34, #reads = 1077028 mem = 536877729 peak = 1092300800 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 285043, 2, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 533, 4078) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11874) [M::mem_pestat] mean and std.dev: (1483.57, 1667.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15772) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 282, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (309.74, 159.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 303, 1126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3014) [M::mem_pestat] mean and std.dev: (522.43, 529.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3958) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689716 sequences (100000176 bp) in 2.123 CPU sec, 2.264 real sec... [M::process] read 688228 sequences (100000223 bp) in 1.370 CPU sec, 1.375 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 286686, 13, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 314, 649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1563) [M::mem_pestat] mean and std.dev: (422.20, 372.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2020) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 267, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (295.24, 150.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1065) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (166, 445, 733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1867) [M::mem_pestat] mean and std.dev: (445.38, 253.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2434) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (249, 360, 564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1194) [M::mem_pestat] mean and std.dev: (312.70, 116.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1509) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689964 reads in 149.698 CPU sec, 4.768 real sec [M::mem_process_seqs] Processed 692726 reads in 94.936 CPU sec, 3.014 real sec sorting block 35, #reads = 1077104 mem = 536881108 peak = 1092300800 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 286154, 6, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 261, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1174) [M::mem_pestat] mean and std.dev: (264.50, 145.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 284, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (308.59, 155.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (276, 404, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (308.93, 106.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687174 sequences (100000049 bp) in 2.421 CPU sec, 2.560 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 286018, 17, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 262, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (222.86, 105.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 296, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (317.07, 158.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (729, 1343, 1939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4359) [M::mem_pestat] mean and std.dev: (1175.41, 686.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5569) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 685754 sequences (100000168 bp) in 1.390 CPU sec, 1.388 real sec... [M::mem_process_seqs] Processed 689716 reads in 146.800 CPU sec, 4.666 real sec sorting block 36, #reads = 1078740 mem = 536879574 peak = 1092300800 [M::mem_process_seqs] Processed 688228 reads in 92.022 CPU sec, 2.914 real sec sorting block 37, #reads = 1078788 mem = 536881219 peak = 1106063360 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284960, 3, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 399, 823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2097) [M::mem_pestat] mean and std.dev: (417.17, 258.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 303, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (321.43, 159.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (249, 359, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (343.32, 132.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684658 sequences (100000081 bp) in 2.005 CPU sec, 2.113 real sec... [M::process] read 683882 sequences (100000077 bp) in 1.380 CPU sec, 1.381 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 284471, 1, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 158, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (226.00, 110.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 311, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937) [M::mem_pestat] mean and std.dev: (329.28, 162.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1185) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (255, 303, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (25, 600) [M::mem_pestat] mean and std.dev: (298.81, 96.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687174 reads in 147.674 CPU sec, 4.711 real sec [M::mem_process_seqs] Processed 685754 reads in 93.966 CPU sec, 2.982 real sec sorting block 38, #reads = 1077681 mem = 536880265 peak = 1106063360 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283952, 4, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 250, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (292.21, 139.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 314, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940) [M::mem_pestat] mean and std.dev: (332.34, 163.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1188) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 653, 835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2127) [M::mem_pestat] mean and std.dev: (499.70, 388.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2773) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683376 sequences (100000095 bp) in 2.271 CPU sec, 2.390 real sec... [M::process] read 682656 sequences (100000185 bp) in 1.360 CPU sec, 1.359 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283530, 7, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 312, 855) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2255) [M::mem_pestat] mean and std.dev: (562.14, 490.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2955) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 318, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952) [M::mem_pestat] mean and std.dev: (336.52, 165.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1203) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 258, 1066) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2826) [M::mem_pestat] mean and std.dev: (529.92, 464.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3706) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684658 reads in 146.914 CPU sec, 4.669 real sec [M::mem_process_seqs] Processed 683882 reads in 91.873 CPU sec, 2.915 real sec sorting block 39, #reads = 1076534 mem = 536878087 peak = 1106063360 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283046, 6, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 195, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (240.32, 117.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 320, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948) [M::mem_pestat] mean and std.dev: (338.00, 165.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1197) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 311, 742) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1772) [M::mem_pestat] mean and std.dev: (386.73, 240.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2287) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688286 sequences (100000286 bp) in 2.012 CPU sec, 2.108 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282921, 5, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (234, 266, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (18, 558) [M::mem_pestat] mean and std.dev: (255.55, 91.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 325, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960) [M::mem_pestat] mean and std.dev: (343.03, 168.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1212) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 303, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (258.59, 93.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 690032 sequences (100000103 bp) in 1.380 CPU sec, 1.376 real sec... [M::mem_process_seqs] Processed 683376 reads in 144.563 CPU sec, 4.612 real sec sorting block 40, #reads = 1076897 mem = 536878358 peak = 1106063360 [M::mem_process_seqs] Processed 682656 reads in 90.191 CPU sec, 2.855 real sec sorting block 41, #reads = 1076158 mem = 536882808 peak = 1106063360 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 284708, 10, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 271, 985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2699) [M::mem_pestat] mean and std.dev: (389.00, 490.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 293, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (317.75, 162.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (939, 1393, 1437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2433) [M::mem_pestat] mean and std.dev: (1145.80, 417.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2931) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 437, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1280) [M::mem_pestat] mean and std.dev: (335.79, 161.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1654) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688038 sequences (100000131 bp) in 2.296 CPU sec, 2.412 real sec... [M::process] read 686738 sequences (100000265 bp) in 1.370 CPU sec, 1.370 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 285258, 12, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 299, 895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2363) [M::mem_pestat] mean and std.dev: (306.67, 243.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3097) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 283, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (308.37, 156.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (559, 559, 1047) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2023) [M::mem_pestat] mean and std.dev: (532.27, 315.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2511) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 214, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (225.20, 92.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688286 reads in 148.225 CPU sec, 4.714 real sec [M::mem_process_seqs] Processed 690032 reads in 89.789 CPU sec, 2.853 real sec sorting block 42, #reads = 1078340 mem = 536881148 peak = 1106063360 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 284355, 1, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 301, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (344.22, 216.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1209) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 295, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (315.49, 156.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 332, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (320.68, 162.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686650 sequences (100000120 bp) in 1.970 CPU sec, 2.066 real sec... [M::process] read 686250 sequences (100000294 bp) in 1.420 CPU sec, 1.420 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 283907, 7, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 219, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1185) [M::mem_pestat] mean and std.dev: (306.92, 157.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 303, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (321.19, 158.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1142) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (276, 322, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (88, 558) [M::mem_pestat] mean and std.dev: (294.82, 95.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688038 reads in 141.168 CPU sec, 4.494 real sec [M::mem_process_seqs] Processed 686738 reads in 88.653 CPU sec, 2.810 real sec sorting block 43, #reads = 1082547 mem = 536878975 peak = 1106063360 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 284019, 3, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 426, 565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1267) [M::mem_pestat] mean and std.dev: (382.23, 182.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 303, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (322.34, 159.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1150) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 421, 857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2155) [M::mem_pestat] mean and std.dev: (517.88, 363.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2804) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685670 sequences (100000162 bp) in 2.488 CPU sec, 2.625 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283645, 6, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 182, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (221.14, 102.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 306, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (322.89, 158.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (139, 184, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (209.20, 97.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684712 sequences (100000020 bp) in 1.370 CPU sec, 1.370 real sec... [M::mem_process_seqs] Processed 686650 reads in 141.176 CPU sec, 4.486 real sec [M::mem_process_seqs] Processed 686250 reads in 88.588 CPU sec, 2.807 real sec sorting block 44, #reads = 1080122 mem = 536877583 peak = 1106063360 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 283719, 2, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 365, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055) [M::mem_pestat] mean and std.dev: (290.31, 137.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1348) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 310, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (326.71, 159.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1162) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 272, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (278.88, 109.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684668 sequences (100000135 bp) in 2.157 CPU sec, 2.288 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 283432, 2, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 306, 1204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3196) [M::mem_pestat] mean and std.dev: (597.95, 582.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4192) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 314, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937) [M::mem_pestat] mean and std.dev: (331.43, 161.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1184) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 303, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1107) [M::mem_pestat] mean and std.dev: (302.64, 174.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1399) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683846 sequences (100000150 bp) in 1.380 CPU sec, 1.385 real sec... [M::mem_process_seqs] Processed 685670 reads in 146.829 CPU sec, 4.681 real sec sorting block 45, #reads = 1080310 mem = 536878695 peak = 1106063360 [M::mem_process_seqs] Processed 684712 reads in 93.962 CPU sec, 2.975 real sec sorting block 46, #reads = 1077224 mem = 536883276 peak = 1106063360 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 283422, 6, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 255, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (89, 399) [M::mem_pestat] mean and std.dev: (228.38, 32.84) [M::mem_pestat] low and high boundaries for proper pairs: (27, 461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 312, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (329.67, 160.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 332, 1231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3393) [M::mem_pestat] mean and std.dev: (660.19, 774.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4474) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683876 sequences (100000135 bp) in 2.266 CPU sec, 2.363 real sec... [M::process] read 695266 sequences (100000093 bp) in 1.440 CPU sec, 1.442 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 283327, 5, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 406, 1206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3214) [M::mem_pestat] mean and std.dev: (721.75, 777.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4218) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 316, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945) [M::mem_pestat] mean and std.dev: (334.60, 163.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1194) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 298, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066) [M::mem_pestat] mean and std.dev: (313.71, 155.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1364) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684668 reads in 149.122 CPU sec, 4.738 real sec [M::mem_process_seqs] Processed 683846 reads in 93.879 CPU sec, 2.980 real sec sorting block 47, #reads = 1074995 mem = 536879901 peak = 1106063360 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 283087, 6, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 334, 959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2485) [M::mem_pestat] mean and std.dev: (493.53, 457.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3248) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 319, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952) [M::mem_pestat] mean and std.dev: (336.90, 165.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1203) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 263, 1168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3206) [M::mem_pestat] mean and std.dev: (417.89, 412.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4225) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 691736 sequences (100000272 bp) in 2.009 CPU sec, 2.092 real sec... [M::process] read 689268 sequences (100000209 bp) in 1.410 CPU sec, 1.414 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 287947, 2, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 243, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (275.35, 138.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 257, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (286.59, 146.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 395, 839) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2043) [M::mem_pestat] mean and std.dev: (470.67, 434.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2645) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683876 reads in 156.017 CPU sec, 4.973 real sec [M::mem_process_seqs] Processed 695266 reads in 97.466 CPU sec, 3.101 real sec sorting block 48, #reads = 1073918 mem = 536878079 peak = 1106063360 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 286024, 5, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 200, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784) [M::mem_pestat] mean and std.dev: (220.45, 95.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 984) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 275, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848) [M::mem_pestat] mean and std.dev: (298.56, 148.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1072) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 382, 580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1416) [M::mem_pestat] mean and std.dev: (411.70, 328.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1834) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688400 sequences (100000003 bp) in 2.488 CPU sec, 2.610 real sec... [M::process] read 687712 sequences (100000158 bp) in 1.400 CPU sec, 1.404 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 285401, 9, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 336, 1099) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2827) [M::mem_pestat] mean and std.dev: (574.92, 487.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 289, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (309.27, 152.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 284, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1260) [M::mem_pestat] mean and std.dev: (257.20, 144.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1632) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691736 reads in 151.558 CPU sec, 4.815 real sec sorting block 49, #reads = 1076431 mem = 536882284 peak = 1106063360 [M::mem_process_seqs] Processed 689268 reads in 95.165 CPU sec, 3.021 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 284942, 6, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 245, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (220.31, 77.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 296, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (316.20, 156.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 219, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (289.38, 142.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1191) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 50, #reads = 1075979 mem = 536877920 peak = 1110929408 [M::process] read 685544 sequences (100000276 bp) in 2.152 CPU sec, 2.272 real sec... [M::process] read 684494 sequences (100000133 bp) in 1.420 CPU sec, 1.422 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 285304, 7, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (229, 249, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (294.20, 94.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 296, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (316.46, 157.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 347, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1127) [M::mem_pestat] mean and std.dev: (343.77, 182.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1432) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688400 reads in 151.797 CPU sec, 4.834 real sec [M::mem_process_seqs] Processed 687712 reads in 95.753 CPU sec, 3.037 real sec sorting block 51, #reads = 1075323 mem = 536883326 peak = 1110929408 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283995, 3, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 383, 735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1889) [M::mem_pestat] mean and std.dev: (400.00, 267.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 306, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (322.99, 156.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 264, 608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1430) [M::mem_pestat] mean and std.dev: (303.95, 168.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1841) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683988 sequences (100000108 bp) in 2.288 CPU sec, 2.386 real sec... [M::process] read 683180 sequences (100000174 bp) in 1.370 CPU sec, 1.372 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 283566, 2, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 273, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027) [M::mem_pestat] mean and std.dev: (311.31, 173.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1309) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 310, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (326.48, 156.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 339, 596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1406) [M::mem_pestat] mean and std.dev: (324.14, 165.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1811) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685544 reads in 149.145 CPU sec, 4.740 real sec [M::mem_process_seqs] Processed 684494 reads in 93.206 CPU sec, 2.967 real sec sorting block 52, #reads = 1075447 mem = 536880810 peak = 1110929408 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 283258, 4, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 281, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (258.90, 117.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 310, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (326.54, 157.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 313, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1199) [M::mem_pestat] mean and std.dev: (371.13, 238.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1539) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682424 sequences (100000178 bp) in 2.124 CPU sec, 2.247 real sec... [M::process] read 3294 sequences (481242 bp) in 0.230 CPU sec, 0.235 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 282572, 10, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 297, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (318.64, 125.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 317, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (335.06, 162.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1173) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (277, 333, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (161, 451) [M::mem_pestat] mean and std.dev: (328.12, 20.62) [M::mem_pestat] low and high boundaries for proper pairs: (103, 509) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 258, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (244.63, 82.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683988 reads in 144.037 CPU sec, 4.619 real sec sorting block 53, #reads = 1076414 mem = 536878407 peak = 1110929408 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 1386, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 299, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (323.18, 159.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1091) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 3294 reads in 0.740 CPU sec, 0.024 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 283418, 2, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 309, 814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2044) [M::mem_pestat] mean and std.dev: (345.79, 236.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 325, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960) [M::mem_pestat] mean and std.dev: (343.04, 168.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1212) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 311, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945) [M::mem_pestat] mean and std.dev: (297.20, 145.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1194) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682424 reads in 76.019 CPU sec, 2.482 real sec [M::mem_process_seqs] Processed 683180 reads in 165.861 CPU sec, 6.046 real sec sorting block 54, #reads = 1075655 mem = 536881060 peak = 1110929408 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 59608420 overall: 70313336832 mem 8074.252 user 306.241 sys 1176.924 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_3U6-T1-TDNA-1_A23MHWWLT4_1.L004\tPU:L004\tSM:1136_3U6-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_3U6-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0001.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0001.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 1176.960 sec; CPU: 8380.496 sec sorting block 55, #reads = 581452 mem = 289922951 peak = 1110929408 uniq header count 1 merging 56 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 3579776815 output reads: 59928219 bam_mem_sort: 56 calls 20.749 user 0.153 sys 21.037 real bam_write: 56 calls 48.310 user 0.952 sys 49.559 real execute: 1 calls 10.649 user 1.015 sys 1197.357 real merge_files: 1 calls 10.633 user 0.992 sys 19.624 real parse_chunk: 889 calls 26.865 user 8.056 sys 35.562 real read_chunk: 2400 calls 22.147 user 21.365 sys 223.589 real sort_block: 1 calls 17.393 user 20.927 sys 216.266 real write_chunk: 1509 calls 10.302 user 0.830 sys 18.927 real overall: 2680320000 mem 732.505 user 38.837 sys 1197.378 real ls: cannot access '*.cram': No such file or directory