.command.log - Workdir - DAQ:SENTIEON_BWAMEM (1136_3U6-T1-TDNA-1_A23MHWWLT4_1;L004;0001)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/40/2f3d91d62b83596c909c19e8e8b32f/.command.log
Size: 126.5 KB
Task: DAQ:SENTIEON_BWAMEM (1136_3U6-T1-TDNA-1_A23MHWWLT4_1;L004;0001)
Attempt
Content

  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/05/c9eeb78a93c7ea5dc26a45152221cb/1136_3U6-T1-TDNA-1_A23MHWWLT4_1/0001.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/40/2f3d91d62b83596c909c19e8e8b32f/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/05/c9eeb78a93c7ea5dc26a45152221cb/1136_3U6-T1-TDNA-1_A23MHWWLT4_1/0001.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/40/2f3d91d62b83596c909c19e8e8b32f/.command.run
==> STAGING COMPLETE (7 inputs)

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_3U6-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
Received only 16 licenses out of 31 requested for sentieon:klib
Received only 16 licenses out of 31 requested for sentieon:klib
[M::main_mem] bwa index loaded in 1658.671 CPU sec, 949.800 real sec
[M::process] read 686960 sequences (100000236 bp) in 0.962 CPU sec, 0.966 real sec...
[M::process] read 686242 sequences (100000258 bp) in 1.040 CPU sec, 1.041 real sec...
[M::process] read 684872 sequences (100000143 bp) in 1.078 CPU sec, 1.110 real sec...
[M::process] read 683706 sequences (100000266 bp) in 1.081 CPU sec, 1.120 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 284159, 5, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (221, 350, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1106)
[M::mem_pestat] mean and std.dev: (377.00, 190.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1401)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 301, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (321.27, 160.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 273, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (236.13, 69.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 692)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 283653, 4, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (226, 272, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (80, 445)
[M::mem_pestat] mean and std.dev: (252.82, 67.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 523)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 314, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (332.09, 163.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 233, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (236.35, 102.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 284139, 7, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 270, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (6, 516)
[M::mem_pestat] mean and std.dev: (219.86, 74.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 618)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 306, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (325.32, 161.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 327, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (269.29, 96.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686960 reads in 191.278 CPU sec, 10.674 real sec
[M::mem_process_seqs] Processed 684872 reads in 139.189 CPU sec, 7.206 real sec
[M::mem_process_seqs] Processed 686242 reads in 151.404 CPU sec, 7.626 real sec
sorting block 0, #reads = 1082081 mem = 536879610 peak = 599572480
[M::process] read 683272 sequences (100000008 bp) in 1.601 CPU sec, 1.861 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282791, 7, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 409, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1190)
[M::mem_pestat] mean and std.dev: (380.79, 164.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1522)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 323, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (340.04, 167.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 297, 607)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1319)
[M::mem_pestat] mean and std.dev: (405.57, 270.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1675)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683260 sequences (100000062 bp) in 1.259 CPU sec, 1.286 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282904, 6, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (205, 293, 893)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2269)
[M::mem_pestat] mean and std.dev: (550.88, 529.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2957)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 324, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (341.09, 167.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 285, 1182)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3198)
[M::mem_pestat] mean and std.dev: (590.33, 580.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4206)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683444 sequences (100000031 bp) in 1.110 CPU sec, 1.110 real sec...
[M::mem_process_seqs] Processed 683706 reads in 136.133 CPU sec, 4.324 real sec
sorting block 1, #reads = 1081012 mem = 536879741 peak = 928002048
[M::mem_process_seqs] Processed 683272 reads in 85.476 CPU sec, 2.711 real sec
sorting block 2, #reads = 1080829 mem = 536879610 peak = 973672448
[M::process] read 682952 sequences (100000067 bp) in 1.560 CPU sec, 1.659 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282777, 7, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (270, 324, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (118, 498)
[M::mem_pestat] mean and std.dev: (285.46, 71.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 574)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 322, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (339.29, 166.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 238, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (256.80, 143.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682260 sequences (100000189 bp) in 1.224 CPU sec, 1.223 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282765, 3, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 305, 826)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2104)
[M::mem_pestat] mean and std.dev: (466.48, 396.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2743)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 319, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (337.04, 165.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 384, 881)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2189)
[M::mem_pestat] mean and std.dev: (493.91, 355.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2843)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 282449, 8, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 221, 242)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446)
[M::mem_pestat] mean and std.dev: (186.75, 47.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 548)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 322, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (339.40, 166.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 274, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (284.25, 110.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683260 reads in 196.415 CPU sec, 6.306 real sec
[M::mem_process_seqs] Processed 683444 reads in 145.458 CPU sec, 4.685 real sec
[M::mem_process_seqs] Processed 682952 reads in 157.553 CPU sec, 5.100 real sec
sorting block 3, #reads = 1080264 mem = 536882616 peak = 973672448
[M::process] read 681824 sequences (100000300 bp) in 1.883 CPU sec, 2.014 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282090, 7, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 292, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (316.06, 151.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 326, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (341.82, 165.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (320, 371, 581)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1103)
[M::mem_pestat] mean and std.dev: (362.87, 136.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1364)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 4, #reads = 1080332 mem = 536877211 peak = 991092736
[M::process] read 681076 sequences (100000207 bp) in 1.403 CPU sec, 1.447 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282200, 3, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 298, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (315.46, 184.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1103)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 329, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (343.95, 165.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 388, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (324.26, 171.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682260 reads in 138.904 CPU sec, 4.428 real sec
[M::mem_process_seqs] Processed 681824 reads in 86.906 CPU sec, 2.740 real sec
[M::process] read 683804 sequences (100000205 bp) in 1.494 CPU sec, 1.549 real sec...
sorting block 5, #reads = 1078688 mem = 536881487 peak = 991092736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 281875, 2, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 413, 728)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1854)
[M::mem_pestat] mean and std.dev: (450.14, 311.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2417)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 334, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (349.40, 167.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 589, 629)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1551)
[M::mem_pestat] mean and std.dev: (543.53, 410.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2187)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686532 sequences (100000076 bp) in 1.865 CPU sec, 1.997 real sec...
[M::process] read 685616 sequences (100000118 bp) in 1.230 CPU sec, 1.227 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 282941, 1, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (226, 234, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (227.67, 75.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 790)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 321, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (338.90, 167.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 260, 570)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1376)
[M::mem_pestat] mean and std.dev: (316.25, 197.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1779)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681076 reads in 134.920 CPU sec, 4.298 real sec
[M::mem_process_seqs] Processed 683804 reads in 85.245 CPU sec, 2.704 real sec
sorting block 6, #reads = 1078957 mem = 536878625 peak = 991092736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283788, 9, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 178, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (260.72, 159.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 307, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (327.06, 164.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 432, 574)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1378)
[M::mem_pestat] mean and std.dev: (340.85, 230.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1780)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684506 sequences (100000093 bp) in 1.701 CPU sec, 1.816 real sec...
[M::process] read 683744 sequences (100000062 bp) in 1.249 CPU sec, 1.252 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 283402, 6, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (230, 352, 718)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1694)
[M::mem_pestat] mean and std.dev: (436.45, 252.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2182)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 313, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (330.59, 164.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 350, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (346.38, 130.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686532 reads in 135.731 CPU sec, 4.328 real sec
[M::mem_process_seqs] Processed 685616 reads in 84.427 CPU sec, 2.686 real sec
sorting block 7, #reads = 1081747 mem = 536877925 peak = 991092736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282713, 5, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 236, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (290.21, 126.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 321, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (337.10, 166.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (321, 349, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861)
[M::mem_pestat] mean and std.dev: (342.71, 133.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683632 sequences (100000283 bp) in 1.995 CPU sec, 2.129 real sec...
[M::process] read 683550 sequences (100000020 bp) in 1.260 CPU sec, 1.264 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 283116, 4, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 238, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (276.96, 124.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 884)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 326, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (341.96, 168.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (129, 205, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1137)
[M::mem_pestat] mean and std.dev: (245.00, 149.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1473)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684506 reads in 134.669 CPU sec, 4.283 real sec
sorting block 8, #reads = 1082680 mem = 536879958 peak = 991092736
[M::mem_process_seqs] Processed 683744 reads in 84.072 CPU sec, 2.669 real sec
sorting block 9, #reads = 1081312 mem = 536879504 peak = 1009647616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 282882, 5, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 314, 712)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1802)
[M::mem_pestat] mean and std.dev: (399.68, 309.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2347)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 326, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (342.69, 169.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 334, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1095)
[M::mem_pestat] mean and std.dev: (338.56, 213.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1406)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683612 sequences (100000082 bp) in 1.926 CPU sec, 2.079 real sec...
[M::process] read 683248 sequences (100000187 bp) in 1.320 CPU sec, 1.317 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 283005, 5, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (246, 473, 604)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1320)
[M::mem_pestat] mean and std.dev: (511.26, 303.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1727)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 322, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (339.95, 167.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 261, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721)
[M::mem_pestat] mean and std.dev: (254.45, 95.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683632 reads in 136.974 CPU sec, 4.361 real sec
[M::mem_process_seqs] Processed 683550 reads in 85.863 CPU sec, 2.722 real sec
sorting block 10, #reads = 1080820 mem = 536880020 peak = 1009647616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282915, 6, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 226, 242)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (80, 350)
[M::mem_pestat] mean and std.dev: (193.86, 50.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 404)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 316, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (334.30, 163.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 262, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1122)
[M::mem_pestat] mean and std.dev: (337.30, 213.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1422)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682614 sequences (100000025 bp) in 2.085 CPU sec, 2.222 real sec...
[M::process] read 681986 sequences (100000264 bp) in 1.310 CPU sec, 1.305 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282884, 6, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 319, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1089)
[M::mem_pestat] mean and std.dev: (312.67, 140.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1389)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 321, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (337.35, 164.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 280, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (307.45, 142.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683612 reads in 136.457 CPU sec, 4.337 real sec
[M::mem_process_seqs] Processed 683248 reads in 85.307 CPU sec, 2.707 real sec
sorting block 11, #reads = 1080558 mem = 536878530 peak = 1009647616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282868, 5, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (197, 262, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (278.50, 131.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 909)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 324, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (341.00, 165.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 370, 1218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3190)
[M::mem_pestat] mean and std.dev: (757.00, 674.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4176)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681784 sequences (100000074 bp) in 1.978 CPU sec, 2.139 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 282202, 9, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (199, 222, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.58, 92.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 328, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (344.70, 167.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 408, 679)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1679)
[M::mem_pestat] mean and std.dev: (408.86, 309.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2179)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687418 sequences (100000162 bp) in 1.330 CPU sec, 1.330 real sec...
[M::mem_process_seqs] Processed 682614 reads in 134.390 CPU sec, 4.280 real sec
[M::mem_process_seqs] Processed 681986 reads in 84.526 CPU sec, 2.677 real sec
sorting block 12, #reads = 1080019 mem = 536878254 peak = 1009647616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282760, 16, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 361, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046)
[M::mem_pestat] mean and std.dev: (354.94, 254.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1375)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 333, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (349.45, 170.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (62, 278, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1382)
[M::mem_pestat] mean and std.dev: (269.53, 234.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1822)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 305, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (279.60, 127.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 976)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 687242 sequences (100000265 bp) in 2.108 CPU sec, 2.226 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 284307, 4, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (359, 415, 614)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1124)
[M::mem_pestat] mean and std.dev: (424.89, 200.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1379)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 303, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (322.27, 161.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (130, 261, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (243.00, 114.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 906)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685774 sequences (100000272 bp) in 1.350 CPU sec, 1.347 real sec...
[M::mem_process_seqs] Processed 681784 reads in 136.234 CPU sec, 4.332 real sec
sorting block 13, #reads = 1079295 mem = 536881799 peak = 1009647616
[M::mem_process_seqs] Processed 687418 reads in 86.008 CPU sec, 2.723 real sec
sorting block 14, #reads = 1080297 mem = 536891435 peak = 1064579072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 284351, 8, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 222, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (81, 326)
[M::mem_pestat] mean and std.dev: (184.55, 39.09)
[M::mem_pestat] low and high boundaries for proper pairs: (28, 375)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 303, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (320.93, 158.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 684638 sequences (100000265 bp) in 1.941 CPU sec, 2.079 real sec...
[M::process] read 683762 sequences (100000059 bp) in 1.360 CPU sec, 1.358 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283838, 5, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 376, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (341.56, 213.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 311, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (327.11, 159.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 297, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (265.74, 106.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687242 reads in 139.059 CPU sec, 4.428 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283002, 3, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (205, 411, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (354.19, 170.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 318, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (332.42, 161.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 325, 538)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1242)
[M::mem_pestat] mean and std.dev: (350.39, 182.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1594)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683424 sequences (100000287 bp) in 1.736 CPU sec, 1.740 real sec...
[M::mem_process_seqs] Processed 684638 reads in 75.756 CPU sec, 2.397 real sec
sorting block 15, #reads = 1082209 mem = 536880634 peak = 1064579072
[M::mem_process_seqs] Processed 685774 reads in 190.345 CPU sec, 6.051 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282566, 4, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (261, 299, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (59, 564)
[M::mem_pestat] mean and std.dev: (290.25, 67.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 665)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 320, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (335.20, 162.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 291, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (277.61, 87.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 16, #reads = 1081875 mem = 536880086 peak = 1064579072
[M::process] read 683322 sequences (100000179 bp) in 1.956 CPU sec, 2.120 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283379, 5, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 243, 638)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1550)
[M::mem_pestat] mean and std.dev: (348.88, 231.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2006)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 323, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (338.14, 163.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 302, 589)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1371)
[M::mem_pestat] mean and std.dev: (340.72, 177.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1762)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683036 sequences (100000058 bp) in 1.330 CPU sec, 1.325 real sec...
[M::mem_process_seqs] Processed 683762 reads in 151.113 CPU sec, 4.803 real sec
[M::mem_process_seqs] Processed 683424 reads in 87.442 CPU sec, 2.762 real sec
sorting block 17, #reads = 1080426 mem = 536883678 peak = 1064579072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282623, 1, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 470, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1229)
[M::mem_pestat] mean and std.dev: (356.63, 204.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1577)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 319, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (335.88, 163.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 304, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (345.38, 168.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683014 sequences (100000134 bp) in 2.362 CPU sec, 2.542 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282570, 5, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 549, 882)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2324)
[M::mem_pestat] mean and std.dev: (459.40, 369.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3045)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 320, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (336.68, 163.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 287, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (267.57, 109.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682600 sequences (100000215 bp) in 1.350 CPU sec, 1.348 real sec...
[M::mem_process_seqs] Processed 683322 reads in 139.960 CPU sec, 4.445 real sec
[M::mem_process_seqs] Processed 683036 reads in 88.530 CPU sec, 2.816 real sec
sorting block 18, #reads = 1080275 mem = 536883462 peak = 1064579072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282550, 7, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 242, 658)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1562)
[M::mem_pestat] mean and std.dev: (524.06, 499.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2523)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 321, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (336.27, 162.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 265, 548)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1174)
[M::mem_pestat] mean and std.dev: (334.18, 161.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1487)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682318 sequences (100000071 bp) in 1.844 CPU sec, 1.968 real sec...
[M::process] read 685424 sequences (100000204 bp) in 1.360 CPU sec, 1.360 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282829, 7, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (306, 306, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861)
[M::mem_pestat] mean and std.dev: (320.45, 122.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 325, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (341.52, 166.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 309, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (300.06, 140.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683014 reads in 139.200 CPU sec, 4.436 real sec
sorting block 19, #reads = 1079019 mem = 536878211 peak = 1064579072
[M::mem_process_seqs] Processed 682600 reads in 87.174 CPU sec, 2.764 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 282640, 4, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 345, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1139)
[M::mem_pestat] mean and std.dev: (291.37, 123.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1457)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 327, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (344.25, 169.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 332, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (282.61, 125.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686614 sequences (100000299 bp) in 2.213 CPU sec, 2.379 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 283551, 1, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 195, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561)
[M::mem_pestat] mean and std.dev: (238.22, 101.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 690)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 311, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (329.58, 163.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 325, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1107)
[M::mem_pestat] mean and std.dev: (360.13, 213.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1408)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685940 sequences (100000213 bp) in 1.350 CPU sec, 1.348 real sec...
[M::mem_process_seqs] Processed 682318 reads in 137.694 CPU sec, 4.378 real sec
sorting block 20, #reads = 1078893 mem = 536879372 peak = 1064579072
[M::mem_process_seqs] Processed 685424 reads in 87.781 CPU sec, 2.785 real sec
sorting block 21, #reads = 1077631 mem = 536879746 peak = 1064579072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 284322, 2, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (261, 547, 876)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2106)
[M::mem_pestat] mean and std.dev: (532.69, 250.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2721)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 305, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (323.56, 160.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 243, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (268.93, 125.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 861)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684538 sequences (100000104 bp) in 1.962 CPU sec, 2.106 real sec...
[M::process] read 684040 sequences (100000003 bp) in 1.340 CPU sec, 1.342 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 283223, 2, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 301, 1166)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3136)
[M::mem_pestat] mean and std.dev: (600.84, 698.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4121)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 310, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (327.31, 161.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 364, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052)
[M::mem_pestat] mean and std.dev: (340.15, 174.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1338)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686614 reads in 138.236 CPU sec, 4.401 real sec
[M::mem_process_seqs] Processed 685940 reads in 87.360 CPU sec, 2.774 real sec
sorting block 22, #reads = 1083262 mem = 536881393 peak = 1064579072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282591, 4, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 431, 860)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2160)
[M::mem_pestat] mean and std.dev: (464.39, 322.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2810)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 318, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (333.93, 163.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (298, 405, 572)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1120)
[M::mem_pestat] mean and std.dev: (383.52, 133.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1394)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684054 sequences (100000104 bp) in 2.185 CPU sec, 2.333 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282036, 4, 23)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 320, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (334.70, 162.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 411, 741)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1803)
[M::mem_pestat] mean and std.dev: (519.74, 358.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2334)
[M::mem_pestat] skip orientation RR
[M::process] read 684678 sequences (100000131 bp) in 1.350 CPU sec, 1.351 real sec...
[M::mem_process_seqs] Processed 684538 reads in 141.109 CPU sec, 4.494 real sec
[M::mem_process_seqs] Processed 684040 reads in 88.992 CPU sec, 2.817 real sec
sorting block 23, #reads = 1082108 mem = 536881564 peak = 1064579072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282572, 4, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 313, 571)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1417)
[M::mem_pestat] mean and std.dev: (327.95, 186.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1840)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 321, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (336.12, 163.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 396, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (365.23, 126.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1082)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684128 sequences (100000019 bp) in 1.983 CPU sec, 2.123 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283780, 5, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (256, 729, 877)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2119)
[M::mem_pestat] mean and std.dev: (728.54, 508.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2761)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 310, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (329.00, 161.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 301, 1078)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2818)
[M::mem_pestat] mean and std.dev: (494.05, 415.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3688)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683714 sequences (100000012 bp) in 1.350 CPU sec, 1.349 real sec...
[M::mem_process_seqs] Processed 684054 reads in 142.116 CPU sec, 4.535 real sec
sorting block 24, #reads = 1080384 mem = 536880307 peak = 1064579072
[M::mem_process_seqs] Processed 684678 reads in 88.781 CPU sec, 2.815 real sec
sorting block 25, #reads = 1079701 mem = 536879087 peak = 1064579072
[M::process] read 683250 sequences (100000137 bp) in 2.254 CPU sec, 2.396 real sec...
[M::process] read 682712 sequences (100000078 bp) in 1.350 CPU sec, 1.348 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282330, 5, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 277, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (240.88, 97.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 313, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (329.46, 158.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 374, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (353.21, 163.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683714 reads in 77.725 CPU sec, 2.452 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282333, 5, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (229, 279, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (304.04, 139.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 893)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 312, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (328.64, 158.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 602, 872)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2218)
[M::mem_pestat] mean and std.dev: (537.25, 366.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2891)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282551, 7, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (245, 389, 832)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2006)
[M::mem_pestat] mean and std.dev: (516.26, 387.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2593)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 315, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (331.32, 158.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 279, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (286.57, 110.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686234 sequences (100000014 bp) in 1.687 CPU sec, 1.687 real sec...
[M::mem_process_seqs] Processed 684128 reads in 219.711 CPU sec, 6.977 real sec
[M::mem_process_seqs] Processed 683250 reads in 101.840 CPU sec, 3.250 real sec
sorting block 26, #reads = 1078183 mem = 536879556 peak = 1064579072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282753, 5, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 180, 716)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1872)
[M::mem_pestat] mean and std.dev: (410.76, 347.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2450)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 322, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (339.46, 164.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 258, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873)
[M::mem_pestat] mean and std.dev: (263.00, 106.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 27, #reads = 1078700 mem = 536881575 peak = 1064579072
[M::process] read 689540 sequences (100000068 bp) in 2.055 CPU sec, 2.216 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 284454, 2, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 226, 581)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1417)
[M::mem_pestat] mean and std.dev: (390.04, 334.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1835)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 305, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (327.43, 165.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 309, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (306.41, 148.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687076 sequences (100000246 bp) in 1.330 CPU sec, 1.331 real sec...
[M::mem_process_seqs] Processed 682712 reads in 140.039 CPU sec, 4.467 real sec
[M::mem_process_seqs] Processed 686234 reads in 89.316 CPU sec, 2.830 real sec
sorting block 28, #reads = 1078486 mem = 536883925 peak = 1064579072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 285507, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 295, 559)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1303)
[M::mem_pestat] mean and std.dev: (287.62, 147.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1675)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 285, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (312.65, 161.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 686048 sequences (100000010 bp) in 2.241 CPU sec, 2.404 real sec...
[M::process] read 684828 sequences (100000020 bp) in 1.370 CPU sec, 1.375 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 284783, 5, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (236, 378, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (316.43, 108.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 964)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 303, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (326.82, 166.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 220, 585)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1463)
[M::mem_pestat] mean and std.dev: (268.38, 196.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1902)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 689540 reads in 140.641 CPU sec, 4.467 real sec
[M::mem_process_seqs] Processed 687076 reads in 86.809 CPU sec, 2.756 real sec
sorting block 29, #reads = 1080792 mem = 536878121 peak = 1064579072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 283835, 4, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 270, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (253.67, 131.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 311, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (331.45, 166.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 269, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (348.58, 236.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684282 sequences (100000129 bp) in 1.949 CPU sec, 2.077 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 283503, 6, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 413, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042)
[M::mem_pestat] mean and std.dev: (376.47, 273.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1472)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 684162 sequences (100000140 bp) in 1.370 CPU sec, 1.373 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (196, 317, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (335.71, 166.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 384, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (360.59, 178.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686048 reads in 142.466 CPU sec, 4.547 real sec
[M::mem_process_seqs] Processed 684828 reads in 90.018 CPU sec, 2.861 real sec
sorting block 30, #reads = 1080552 mem = 536879702 peak = 1064579072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 283331, 3, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 354, 984)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2626)
[M::mem_pestat] mean and std.dev: (660.56, 640.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3447)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 319, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (336.75, 165.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 260, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (309.62, 145.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683912 sequences (100000169 bp) in 2.153 CPU sec, 2.276 real sec...
[M::process] read 683652 sequences (100000017 bp) in 1.390 CPU sec, 1.389 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283201, 4, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (221, 433, 768)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1862)
[M::mem_pestat] mean and std.dev: (594.18, 467.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2462)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 319, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (336.20, 165.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 190, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (289.69, 172.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684282 reads in 143.842 CPU sec, 4.574 real sec
sorting block 31, #reads = 1078367 mem = 536880322 peak = 1064579072
[M::mem_process_seqs] Processed 684162 reads in 89.911 CPU sec, 2.857 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 283291, 4, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 215, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (226.54, 88.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 317, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (335.18, 164.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 374, 580)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1252)
[M::mem_pestat] mean and std.dev: (405.72, 195.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1588)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 32, #reads = 1078104 mem = 536879092 peak = 1092300800
[M::process] read 683270 sequences (100000289 bp) in 1.940 CPU sec, 2.055 real sec...
[M::process] read 683074 sequences (100000215 bp) in 1.400 CPU sec, 1.398 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283067, 10, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 284, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1210)
[M::mem_pestat] mean and std.dev: (294.40, 154.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1551)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 318, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (335.22, 163.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (267, 327, 1268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3270)
[M::mem_pestat] mean and std.dev: (648.70, 548.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4271)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 361, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (299.53, 110.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 876)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683912 reads in 145.029 CPU sec, 4.627 real sec
[M::mem_process_seqs] Processed 683652 reads in 91.552 CPU sec, 2.901 real sec
sorting block 33, #reads = 1077274 mem = 536878896 peak = 1092300800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 283457, 3, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 373, 737)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1849)
[M::mem_pestat] mean and std.dev: (423.30, 275.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2405)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 320, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (337.09, 164.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 258, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590)
[M::mem_pestat] mean and std.dev: (266.47, 77.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 713)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 689964 sequences (100000244 bp) in 2.002 CPU sec, 2.124 real sec...
[M::process] read 692726 sequences (100000060 bp) in 1.420 CPU sec, 1.424 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283145, 3, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (240, 320, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (333.54, 143.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 324, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (341.54, 167.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 398, 578)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1286)
[M::mem_pestat] mean and std.dev: (376.00, 208.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1640)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683270 reads in 143.327 CPU sec, 4.563 real sec
[M::mem_process_seqs] Processed 683074 reads in 90.015 CPU sec, 2.854 real sec
sorting block 34, #reads = 1077028 mem = 536877729 peak = 1092300800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 285043, 2, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 533, 4078)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11874)
[M::mem_pestat] mean and std.dev: (1483.57, 1667.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 15772)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 282, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (309.74, 159.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 303, 1126)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3014)
[M::mem_pestat] mean and std.dev: (522.43, 529.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3958)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 689716 sequences (100000176 bp) in 2.123 CPU sec, 2.264 real sec...
[M::process] read 688228 sequences (100000223 bp) in 1.370 CPU sec, 1.375 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 286686, 13, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 314, 649)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1563)
[M::mem_pestat] mean and std.dev: (422.20, 372.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2020)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 267, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (295.24, 150.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 445, 733)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1867)
[M::mem_pestat] mean and std.dev: (445.38, 253.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2434)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 360, 564)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1194)
[M::mem_pestat] mean and std.dev: (312.70, 116.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1509)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 689964 reads in 149.698 CPU sec, 4.768 real sec
[M::mem_process_seqs] Processed 692726 reads in 94.936 CPU sec, 3.014 real sec
sorting block 35, #reads = 1077104 mem = 536881108 peak = 1092300800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 286154, 6, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 261, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1174)
[M::mem_pestat] mean and std.dev: (264.50, 145.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1519)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 284, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (308.59, 155.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (276, 404, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690)
[M::mem_pestat] mean and std.dev: (308.93, 106.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687174 sequences (100000049 bp) in 2.421 CPU sec, 2.560 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 286018, 17, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 262, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562)
[M::mem_pestat] mean and std.dev: (222.86, 105.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 703)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 296, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (317.07, 158.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (729, 1343, 1939)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4359)
[M::mem_pestat] mean and std.dev: (1175.41, 686.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5569)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::process] read 685754 sequences (100000168 bp) in 1.390 CPU sec, 1.388 real sec...
[M::mem_process_seqs] Processed 689716 reads in 146.800 CPU sec, 4.666 real sec
sorting block 36, #reads = 1078740 mem = 536879574 peak = 1092300800
[M::mem_process_seqs] Processed 688228 reads in 92.022 CPU sec, 2.914 real sec
sorting block 37, #reads = 1078788 mem = 536881219 peak = 1106063360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284960, 3, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 399, 823)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2097)
[M::mem_pestat] mean and std.dev: (417.17, 258.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2734)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 303, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (321.43, 159.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 359, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (343.32, 132.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1061)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684658 sequences (100000081 bp) in 2.005 CPU sec, 2.113 real sec...
[M::process] read 683882 sequences (100000077 bp) in 1.380 CPU sec, 1.381 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 284471, 1, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 158, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (226.00, 110.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 311, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (329.28, 162.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 303, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (25, 600)
[M::mem_pestat] mean and std.dev: (298.81, 96.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 715)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687174 reads in 147.674 CPU sec, 4.711 real sec
[M::mem_process_seqs] Processed 685754 reads in 93.966 CPU sec, 2.982 real sec
sorting block 38, #reads = 1077681 mem = 536880265 peak = 1106063360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283952, 4, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 250, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690)
[M::mem_pestat] mean and std.dev: (292.21, 139.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 314, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (332.34, 163.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 653, 835)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2127)
[M::mem_pestat] mean and std.dev: (499.70, 388.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2773)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683376 sequences (100000095 bp) in 2.271 CPU sec, 2.390 real sec...
[M::process] read 682656 sequences (100000185 bp) in 1.360 CPU sec, 1.359 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283530, 7, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 312, 855)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2255)
[M::mem_pestat] mean and std.dev: (562.14, 490.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2955)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 318, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (336.52, 165.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 258, 1066)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2826)
[M::mem_pestat] mean and std.dev: (529.92, 464.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3706)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684658 reads in 146.914 CPU sec, 4.669 real sec
[M::mem_process_seqs] Processed 683882 reads in 91.873 CPU sec, 2.915 real sec
sorting block 39, #reads = 1076534 mem = 536878087 peak = 1106063360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283046, 6, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 195, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (240.32, 117.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 320, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (338.00, 165.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 311, 742)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1772)
[M::mem_pestat] mean and std.dev: (386.73, 240.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2287)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 688286 sequences (100000286 bp) in 2.012 CPU sec, 2.108 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282921, 5, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (234, 266, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (18, 558)
[M::mem_pestat] mean and std.dev: (255.55, 91.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 666)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 325, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (343.03, 168.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 303, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (258.59, 93.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 690032 sequences (100000103 bp) in 1.380 CPU sec, 1.376 real sec...
[M::mem_process_seqs] Processed 683376 reads in 144.563 CPU sec, 4.612 real sec
sorting block 40, #reads = 1076897 mem = 536878358 peak = 1106063360
[M::mem_process_seqs] Processed 682656 reads in 90.191 CPU sec, 2.855 real sec
sorting block 41, #reads = 1076158 mem = 536882808 peak = 1106063360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 284708, 10, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 271, 985)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2699)
[M::mem_pestat] mean and std.dev: (389.00, 490.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3556)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 293, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (317.75, 162.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (939, 1393, 1437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2433)
[M::mem_pestat] mean and std.dev: (1145.80, 417.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2931)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 437, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1280)
[M::mem_pestat] mean and std.dev: (335.79, 161.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1654)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 688038 sequences (100000131 bp) in 2.296 CPU sec, 2.412 real sec...
[M::process] read 686738 sequences (100000265 bp) in 1.370 CPU sec, 1.370 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 285258, 12, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 299, 895)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2363)
[M::mem_pestat] mean and std.dev: (306.67, 243.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3097)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 283, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (308.37, 156.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (559, 559, 1047)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2023)
[M::mem_pestat] mean and std.dev: (532.27, 315.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2511)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 214, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537)
[M::mem_pestat] mean and std.dev: (225.20, 92.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 653)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688286 reads in 148.225 CPU sec, 4.714 real sec
[M::mem_process_seqs] Processed 690032 reads in 89.789 CPU sec, 2.853 real sec
sorting block 42, #reads = 1078340 mem = 536881148 peak = 1106063360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 284355, 1, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 301, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (344.22, 216.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 295, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (315.49, 156.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 332, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (320.68, 162.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686650 sequences (100000120 bp) in 1.970 CPU sec, 2.066 real sec...
[M::process] read 686250 sequences (100000294 bp) in 1.420 CPU sec, 1.420 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 283907, 7, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (213, 219, 537)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1185)
[M::mem_pestat] mean and std.dev: (306.92, 157.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1509)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 303, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (321.19, 158.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (276, 322, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (88, 558)
[M::mem_pestat] mean and std.dev: (294.82, 95.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 676)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688038 reads in 141.168 CPU sec, 4.494 real sec
[M::mem_process_seqs] Processed 686738 reads in 88.653 CPU sec, 2.810 real sec
sorting block 43, #reads = 1082547 mem = 536878975 peak = 1106063360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 284019, 3, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (214, 426, 565)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1267)
[M::mem_pestat] mean and std.dev: (382.23, 182.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1618)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 303, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (322.34, 159.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 421, 857)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2155)
[M::mem_pestat] mean and std.dev: (517.88, 363.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2804)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685670 sequences (100000162 bp) in 2.488 CPU sec, 2.625 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283645, 6, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 182, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493)
[M::mem_pestat] mean and std.dev: (221.14, 102.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 630)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 306, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (322.89, 158.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (139, 184, 277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553)
[M::mem_pestat] mean and std.dev: (209.20, 97.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 691)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684712 sequences (100000020 bp) in 1.370 CPU sec, 1.370 real sec...
[M::mem_process_seqs] Processed 686650 reads in 141.176 CPU sec, 4.486 real sec
[M::mem_process_seqs] Processed 686250 reads in 88.588 CPU sec, 2.807 real sec
sorting block 44, #reads = 1080122 mem = 536877583 peak = 1106063360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 283719, 2, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 365, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055)
[M::mem_pestat] mean and std.dev: (290.31, 137.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1348)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 310, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (326.71, 159.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 272, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (278.88, 109.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 905)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684668 sequences (100000135 bp) in 2.157 CPU sec, 2.288 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 283432, 2, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (208, 306, 1204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3196)
[M::mem_pestat] mean and std.dev: (597.95, 582.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4192)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 314, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (331.43, 161.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 303, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1107)
[M::mem_pestat] mean and std.dev: (302.64, 174.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1399)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683846 sequences (100000150 bp) in 1.380 CPU sec, 1.385 real sec...
[M::mem_process_seqs] Processed 685670 reads in 146.829 CPU sec, 4.681 real sec
sorting block 45, #reads = 1080310 mem = 536878695 peak = 1106063360
[M::mem_process_seqs] Processed 684712 reads in 93.962 CPU sec, 2.975 real sec
sorting block 46, #reads = 1077224 mem = 536883276 peak = 1106063360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 283422, 6, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (213, 255, 275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (89, 399)
[M::mem_pestat] mean and std.dev: (228.38, 32.84)
[M::mem_pestat] low and high boundaries for proper pairs: (27, 461)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 312, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (329.67, 160.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 332, 1231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3393)
[M::mem_pestat] mean and std.dev: (660.19, 774.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4474)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683876 sequences (100000135 bp) in 2.266 CPU sec, 2.363 real sec...
[M::process] read 695266 sequences (100000093 bp) in 1.440 CPU sec, 1.442 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 283327, 5, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 406, 1206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3214)
[M::mem_pestat] mean and std.dev: (721.75, 777.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4218)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 316, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (334.60, 163.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 298, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066)
[M::mem_pestat] mean and std.dev: (313.71, 155.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1364)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684668 reads in 149.122 CPU sec, 4.738 real sec
[M::mem_process_seqs] Processed 683846 reads in 93.879 CPU sec, 2.980 real sec
sorting block 47, #reads = 1074995 mem = 536879901 peak = 1106063360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 283087, 6, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (196, 334, 959)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2485)
[M::mem_pestat] mean and std.dev: (493.53, 457.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3248)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 319, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (336.90, 165.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 263, 1168)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3206)
[M::mem_pestat] mean and std.dev: (417.89, 412.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4225)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 691736 sequences (100000272 bp) in 2.009 CPU sec, 2.092 real sec...
[M::process] read 689268 sequences (100000209 bp) in 1.410 CPU sec, 1.414 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 287947, 2, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 243, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (275.35, 138.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 257, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (286.59, 146.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 395, 839)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2043)
[M::mem_pestat] mean and std.dev: (470.67, 434.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2645)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683876 reads in 156.017 CPU sec, 4.973 real sec
[M::mem_process_seqs] Processed 695266 reads in 97.466 CPU sec, 3.101 real sec
sorting block 48, #reads = 1073918 mem = 536878079 peak = 1106063360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 286024, 5, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 200, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (220.45, 95.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 984)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 275, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (298.56, 148.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 382, 580)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1416)
[M::mem_pestat] mean and std.dev: (411.70, 328.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1834)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 688400 sequences (100000003 bp) in 2.488 CPU sec, 2.610 real sec...
[M::process] read 687712 sequences (100000158 bp) in 1.400 CPU sec, 1.404 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 285401, 9, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (235, 336, 1099)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2827)
[M::mem_pestat] mean and std.dev: (574.92, 487.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3691)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 289, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881)
[M::mem_pestat] mean and std.dev: (309.27, 152.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 284, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1260)
[M::mem_pestat] mean and std.dev: (257.20, 144.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1632)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 691736 reads in 151.558 CPU sec, 4.815 real sec
sorting block 49, #reads = 1076431 mem = 536882284 peak = 1106063360
[M::mem_process_seqs] Processed 689268 reads in 95.165 CPU sec, 3.021 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 284942, 6, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 245, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508)
[M::mem_pestat] mean and std.dev: (220.31, 77.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 631)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 296, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (316.20, 156.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 219, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (289.38, 142.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 50, #reads = 1075979 mem = 536877920 peak = 1110929408
[M::process] read 685544 sequences (100000276 bp) in 2.152 CPU sec, 2.272 real sec...
[M::process] read 684494 sequences (100000133 bp) in 1.420 CPU sec, 1.422 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 285304, 7, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (229, 249, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (294.20, 94.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 893)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 296, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (316.46, 157.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 347, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1127)
[M::mem_pestat] mean and std.dev: (343.77, 182.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1432)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688400 reads in 151.797 CPU sec, 4.834 real sec
[M::mem_process_seqs] Processed 687712 reads in 95.753 CPU sec, 3.037 real sec
sorting block 51, #reads = 1075323 mem = 536883326 peak = 1110929408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283995, 3, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 383, 735)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1889)
[M::mem_pestat] mean and std.dev: (400.00, 267.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2466)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 306, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (322.99, 156.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 264, 608)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1430)
[M::mem_pestat] mean and std.dev: (303.95, 168.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1841)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683988 sequences (100000108 bp) in 2.288 CPU sec, 2.386 real sec...
[M::process] read 683180 sequences (100000174 bp) in 1.370 CPU sec, 1.372 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 283566, 2, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 273, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (311.31, 173.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1309)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 310, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (326.48, 156.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 339, 596)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1406)
[M::mem_pestat] mean and std.dev: (324.14, 165.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1811)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685544 reads in 149.145 CPU sec, 4.740 real sec
[M::mem_process_seqs] Processed 684494 reads in 93.206 CPU sec, 2.967 real sec
sorting block 52, #reads = 1075447 mem = 536880810 peak = 1110929408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 283258, 4, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (208, 281, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (258.90, 117.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 310, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (326.54, 157.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 313, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1199)
[M::mem_pestat] mean and std.dev: (371.13, 238.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1539)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682424 sequences (100000178 bp) in 2.124 CPU sec, 2.247 real sec...
[M::process] read 3294 sequences (481242 bp) in 0.230 CPU sec, 0.235 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 282572, 10, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (231, 297, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (318.64, 125.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 317, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (335.06, 162.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (277, 333, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (161, 451)
[M::mem_pestat] mean and std.dev: (328.12, 20.62)
[M::mem_pestat] low and high boundaries for proper pairs: (103, 509)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 258, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520)
[M::mem_pestat] mean and std.dev: (244.63, 82.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 629)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683988 reads in 144.037 CPU sec, 4.619 real sec
sorting block 53, #reads = 1076414 mem = 536878407 peak = 1110929408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 1386, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 299, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868)
[M::mem_pestat] mean and std.dev: (323.18, 159.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1091)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 3294 reads in 0.740 CPU sec, 0.024 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 283418, 2, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (199, 309, 814)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2044)
[M::mem_pestat] mean and std.dev: (345.79, 236.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2659)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 325, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (343.04, 168.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 311, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (297.20, 145.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682424 reads in 76.019 CPU sec, 2.482 real sec
[M::mem_process_seqs] Processed 683180 reads in 165.861 CPU sec, 6.046 real sec
sorting block 54, #reads = 1075655 mem = 536881060 peak = 1110929408
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 59608420
overall: 70313336832 mem 8074.252 user 306.241 sys 1176.924 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_3U6-T1-TDNA-1_A23MHWWLT4_1.L004\tPU:L004\tSM:1136_3U6-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_3U6-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0001.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0001.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 1176.960 sec; CPU: 8380.496 sec
sorting block 55, #reads = 581452 mem = 289922951 peak = 1110929408
uniq header count 1
merging 56 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3579776815
output reads: 59928219
bam_mem_sort: 56 calls 20.749 user 0.153 sys 21.037 real
bam_write: 56 calls 48.310 user 0.952 sys 49.559 real
execute: 1 calls 10.649 user 1.015 sys 1197.357 real
merge_files: 1 calls 10.633 user 0.992 sys 19.624 real
parse_chunk: 889 calls 26.865 user 8.056 sys 35.562 real
read_chunk: 2400 calls 22.147 user 21.365 sys 223.589 real
sort_block: 1 calls 17.393 user 20.927 sys 216.266 real
write_chunk: 1509 calls 10.302 user 0.830 sys 18.927 real
overall: 2680320000 mem 732.505 user 38.837 sys 1197.378 real
ls: cannot access '*.cram': No such file or directory