This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_7OW-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1957.109 CPU sec, 85.297 real sec [M::process] read 687606 sequences (100000148 bp) in 1.677 CPU sec, 1.679 real sec... [M::process] read 687170 sequences (100000066 bp) in 1.822 CPU sec, 2.161 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 283873, 6, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 176, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (203.56, 122.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 293, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (315.03, 155.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 328, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048) [M::mem_pestat] mean and std.dev: (324.80, 133.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1326) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686446 sequences (100000117 bp) in 1.872 CPU sec, 2.255 real sec... [M::process] read 685986 sequences (100000257 bp) in 1.838 CPU sec, 2.037 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 283834, 10, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 296, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (281.06, 119.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 298, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (318.70, 156.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (180, 739, 739) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1857) [M::mem_pestat] mean and std.dev: (559.70, 274.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2416) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 265, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (294.21, 107.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687606 reads in 212.014 CPU sec, 8.161 real sec [M::mem_process_seqs] Processed 687170 reads in 141.812 CPU sec, 6.052 real sec sorting block 0, #reads = 1080778 mem = 536879458 peak = 555999232 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283571, 5, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (275, 312, 882) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2096) [M::mem_pestat] mean and std.dev: (506.21, 417.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 300, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (320.23, 156.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 201, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (7, 407) [M::mem_pestat] mean and std.dev: (204.75, 69.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685516 sequences (100000122 bp) in 2.311 CPU sec, 2.712 real sec... [M::process] read 684778 sequences (100000175 bp) in 1.928 CPU sec, 2.078 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 283815, 10, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 271, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (279.36, 131.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1086) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 303, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (321.69, 156.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 454, 931) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2727) [M::mem_pestat] mean and std.dev: (719.20, 881.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4245) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 350, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960) [M::mem_pestat] mean and std.dev: (340.79, 195.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1211) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686446 reads in 217.212 CPU sec, 8.668 real sec [M::mem_process_seqs] Processed 685986 reads in 143.387 CPU sec, 6.136 real sec sorting block 1, #reads = 1081074 mem = 536880377 peak = 688119808 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 283396, 8, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 291, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959) [M::mem_pestat] mean and std.dev: (284.64, 127.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1211) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 307, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (325.79, 158.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 361, 640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1470) [M::mem_pestat] mean and std.dev: (415.18, 250.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1885) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684628 sequences (100000072 bp) in 2.327 CPU sec, 2.805 real sec... [M::process] read 684196 sequences (100000070 bp) in 2.078 CPU sec, 2.258 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282556, 4, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 316, 541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1219) [M::mem_pestat] mean and std.dev: (323.52, 165.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 308, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (325.95, 157.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (248, 261, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (66, 521) [M::mem_pestat] mean and std.dev: (261.98, 86.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685516 reads in 229.481 CPU sec, 8.791 real sec [M::mem_process_seqs] Processed 684778 reads in 151.863 CPU sec, 5.806 real sec sorting block 2, #reads = 1081080 mem = 536877457 peak = 788193280 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 283606, 7, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 246, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1049) [M::mem_pestat] mean and std.dev: (315.29, 205.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1334) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 309, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (327.22, 157.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 285, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (264.62, 157.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1030) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683580 sequences (100000250 bp) in 2.751 CPU sec, 3.131 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282597, 8, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 290, 812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2026) [M::mem_pestat] mean and std.dev: (382.44, 260.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 313, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (330.05, 158.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1142) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 312, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (307.67, 104.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683220 sequences (100000019 bp) in 2.076 CPU sec, 2.102 real sec... [M::mem_process_seqs] Processed 684628 reads in 224.859 CPU sec, 8.632 real sec sorting block 3, #reads = 1080143 mem = 536879044 peak = 788193280 [M::mem_process_seqs] Processed 684196 reads in 146.453 CPU sec, 5.643 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 282666, 7, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 265, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1159) [M::mem_pestat] mean and std.dev: (309.61, 154.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 318, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (335.24, 160.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 282, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1150) [M::mem_pestat] mean and std.dev: (310.56, 171.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1480) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683254 sequences (100000166 bp) in 2.556 CPU sec, 3.043 real sec... sorting block 4, #reads = 1079989 mem = 536878605 peak = 819286016 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 281698, 17, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 244, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (284.91, 134.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1019) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 320, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (336.91, 161.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1163) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (427, 606, 1822) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4612) [M::mem_pestat] mean and std.dev: (873.76, 593.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6007) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 241, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (260.41, 132.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682790 sequences (100000007 bp) in 2.005 CPU sec, 2.189 real sec... [M::mem_process_seqs] Processed 683580 reads in 220.678 CPU sec, 8.037 real sec [M::mem_process_seqs] Processed 683220 reads in 147.217 CPU sec, 5.382 real sec sorting block 5, #reads = 1078209 mem = 536878266 peak = 819286016 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282046, 1, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 204, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (253.41, 160.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 318, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (335.58, 160.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1158) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 248, 647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1615) [M::mem_pestat] mean and std.dev: (356.80, 297.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2099) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683368 sequences (100000262 bp) in 2.815 CPU sec, 3.168 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 282176, 8, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 272, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1185) [M::mem_pestat] mean and std.dev: (309.98, 207.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 318, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (334.95, 158.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (315, 406, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 597) [M::mem_pestat] mean and std.dev: (358.74, 92.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683392 sequences (100000177 bp) in 2.252 CPU sec, 2.306 real sec... [M::mem_process_seqs] Processed 683254 reads in 227.445 CPU sec, 8.410 real sec [M::mem_process_seqs] Processed 682790 reads in 149.713 CPU sec, 5.647 real sec sorting block 6, #reads = 1078639 mem = 536877530 peak = 819286016 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 281740, 3, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (222, 331, 699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1653) [M::mem_pestat] mean and std.dev: (342.00, 204.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2130) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 316, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (332.94, 158.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1142) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 272, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (318.40, 191.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683556 sequences (100000222 bp) in 2.560 CPU sec, 3.010 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 282223, 4, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 220, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (245.00, 101.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 317, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (335.33, 161.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1165) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 215, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (239.00, 113.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682910 sequences (100000059 bp) in 1.997 CPU sec, 2.168 real sec... [M::mem_process_seqs] Processed 683368 reads in 202.083 CPU sec, 6.846 real sec [M::mem_process_seqs] Processed 683392 reads in 135.535 CPU sec, 4.709 real sec sorting block 7, #reads = 1079518 mem = 536879894 peak = 819286016 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282608, 17, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 530, 1362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3698) [M::mem_pestat] mean and std.dev: (645.96, 608.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4866) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 312, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (331.19, 159.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1143) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (174, 359, 683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1701) [M::mem_pestat] mean and std.dev: (310.00, 196.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2210) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 280, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951) [M::mem_pestat] mean and std.dev: (320.69, 162.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1204) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682650 sequences (100000285 bp) in 2.878 CPU sec, 3.364 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282357, 2, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 382, 556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1244) [M::mem_pestat] mean and std.dev: (358.74, 199.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 318, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (335.32, 159.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1151) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 312, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (314.85, 132.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1014) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683556 reads in 201.807 CPU sec, 6.884 real sec [M::process] read 682686 sequences (100000178 bp) in 2.277 CPU sec, 2.433 real sec... sorting block 8, #reads = 1078038 mem = 536881379 peak = 819286016 [M::mem_process_seqs] Processed 682910 reads in 136.300 CPU sec, 4.911 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 282111, 26, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 247, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (247.17, 125.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 322, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (338.56, 161.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1164) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (367, 367, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 865) [M::mem_pestat] mean and std.dev: (368.47, 94.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1031) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 270, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1064) [M::mem_pestat] mean and std.dev: (304.17, 199.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1360) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 9, #reads = 1079332 mem = 536881690 peak = 854794240 [M::process] read 682570 sequences (100000268 bp) in 2.618 CPU sec, 2.989 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282362, 7, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 177, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (262.50, 193.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 317, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (333.75, 157.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1136) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (271, 350, 925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2233) [M::mem_pestat] mean and std.dev: (476.65, 301.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2887) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682650 reads in 202.420 CPU sec, 7.095 real sec [M::process] read 682920 sequences (100000172 bp) in 2.212 CPU sec, 2.323 real sec... [M::mem_process_seqs] Processed 682686 reads in 137.620 CPU sec, 5.112 real sec sorting block 10, #reads = 1078350 mem = 536878140 peak = 854794240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 281771, 8, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 347, 597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1317) [M::mem_pestat] mean and std.dev: (449.53, 321.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1736) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 317, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (333.82, 157.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1143) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 313, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1152) [M::mem_pestat] mean and std.dev: (352.89, 200.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1468) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682500 sequences (100000025 bp) in 2.877 CPU sec, 3.329 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282022, 3, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 224, 1693) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4777) [M::mem_pestat] mean and std.dev: (808.55, 1019.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6319) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 314, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (330.60, 155.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 245, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (250.94, 85.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682570 reads in 203.535 CPU sec, 7.045 real sec [M::process] read 682168 sequences (100000295 bp) in 2.348 CPU sec, 2.734 real sec... [M::mem_process_seqs] Processed 682920 reads in 138.865 CPU sec, 5.087 real sec sorting block 11, #reads = 1078595 mem = 536878047 peak = 854794240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 281163, 12, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 261, 1006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2634) [M::mem_pestat] mean and std.dev: (621.38, 654.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 316, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (332.41, 156.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (119, 445, 588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1526) [M::mem_pestat] mean and std.dev: (387.92, 336.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1995) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 268, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (299.63, 139.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683072 sequences (100000184 bp) in 2.768 CPU sec, 3.379 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 281746, 3, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 184, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (184.70, 48.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 317, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (334.07, 156.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (115, 263, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (278.83, 180.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1031) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682500 reads in 209.132 CPU sec, 7.133 real sec [M::process] read 683194 sequences (100000081 bp) in 2.259 CPU sec, 2.444 real sec... [M::mem_process_seqs] Processed 682168 reads in 142.462 CPU sec, 4.833 real sec sorting block 12, #reads = 1077658 mem = 536880022 peak = 854794240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 281365, 13, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 298, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (269.21, 98.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 313, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (330.05, 156.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (570, 1140, 1595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3645) [M::mem_pestat] mean and std.dev: (1166.92, 762.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4670) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 346, 801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2051) [M::mem_pestat] mean and std.dev: (465.31, 376.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2676) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682724 sequences (100000236 bp) in 2.965 CPU sec, 3.478 real sec... sorting block 13, #reads = 1076387 mem = 536878593 peak = 901812224 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 282510, 7, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 192, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (223.81, 109.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 312, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (329.31, 156.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 274, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (301.72, 131.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683072 reads in 208.102 CPU sec, 7.050 real sec [M::mem_process_seqs] Processed 683194 reads in 140.825 CPU sec, 4.874 real sec [M::process] read 682632 sequences (100000038 bp) in 2.711 CPU sec, 2.811 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 281642, 14, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 214, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987) [M::mem_pestat] mean and std.dev: (259.35, 175.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1266) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 314, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (331.45, 157.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (836, 868, 3824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9800) [M::mem_pestat] mean and std.dev: (2262.43, 2118.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12788) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 293, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (280.59, 120.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 14, #reads = 1076481 mem = 536877240 peak = 901812224 [M::process] read 682800 sequences (100000170 bp) in 2.591 CPU sec, 2.941 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 281761, 13, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 236, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (276.04, 133.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 315, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (332.53, 157.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (322, 742, 1278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3190) [M::mem_pestat] mean and std.dev: (650.64, 356.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4146) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 300, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (282.94, 119.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682724 reads in 207.033 CPU sec, 6.849 real sec [M::process] read 682558 sequences (100000077 bp) in 2.374 CPU sec, 2.528 real sec... [M::mem_process_seqs] Processed 682632 reads in 141.944 CPU sec, 4.692 real sec sorting block 15, #reads = 1076473 mem = 536878884 peak = 901812224 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 282015, 20, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 301, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060) [M::mem_pestat] mean and std.dev: (299.39, 166.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1358) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 314, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (331.63, 157.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (359, 1508, 1829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4769) [M::mem_pestat] mean and std.dev: (1085.37, 766.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6239) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 341, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1080) [M::mem_pestat] mean and std.dev: (311.22, 109.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1368) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682616 sequences (100000294 bp) in 2.990 CPU sec, 3.302 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 282166, 6, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 289, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (303.26, 167.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1179) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 316, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (334.20, 159.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1143) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 206, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (225.48, 106.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682800 reads in 204.687 CPU sec, 6.644 real sec [M::mem_process_seqs] Processed 682558 reads in 139.874 CPU sec, 4.455 real sec [M::process] read 682112 sequences (100000182 bp) in 2.568 CPU sec, 2.641 real sec... sorting block 16, #reads = 1076990 mem = 536878884 peak = 901812224 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 281877, 10, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 218, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (226.24, 98.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 319, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (337.19, 161.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (695, 815, 1860) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4190) [M::mem_pestat] mean and std.dev: (1044.70, 582.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5355) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (244, 368, 595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1297) [M::mem_pestat] mean and std.dev: (361.03, 223.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1648) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682334 sequences (100000049 bp) in 2.860 CPU sec, 3.129 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 281582, 5, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 334, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987) [M::mem_pestat] mean and std.dev: (313.48, 127.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1248) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 324, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (341.36, 163.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (238, 384, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066) [M::mem_pestat] mean and std.dev: (349.64, 196.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1342) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682616 reads in 203.806 CPU sec, 6.586 real sec [M::process] read 687566 sequences (100000177 bp) in 2.458 CPU sec, 2.511 real sec... [M::mem_process_seqs] Processed 682112 reads in 139.274 CPU sec, 4.471 real sec sorting block 17, #reads = 1078117 mem = 536881239 peak = 901812224 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 281901, 7, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 224, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (236.50, 99.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 320, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (338.39, 162.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (288, 361, 506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942) [M::mem_pestat] mean and std.dev: (353.98, 143.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686706 sequences (100000020 bp) in 3.261 CPU sec, 3.614 real sec... sorting block 18, #reads = 1075562 mem = 536877096 peak = 901812224 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 283717, 7, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 316, 820) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2200) [M::mem_pestat] mean and std.dev: (393.15, 367.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2890) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 293, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (313.29, 153.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1090) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 260, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (248.68, 104.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682334 reads in 203.806 CPU sec, 6.636 real sec [M::mem_process_seqs] Processed 687566 reads in 138.492 CPU sec, 4.459 real sec [M::process] read 685764 sequences (100000109 bp) in 3.099 CPU sec, 3.261 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 283284, 6, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 181, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (192.68, 80.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 299, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (319.71, 156.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (283, 320, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (330.91, 146.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1083) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 19, #reads = 1077399 mem = 536879341 peak = 901812224 [M::process] read 685298 sequences (100000274 bp) in 3.102 CPU sec, 3.322 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 282825, 9, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 291, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (272.93, 112.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 302, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (321.08, 155.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 253, 604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1458) [M::mem_pestat] mean and std.dev: (374.31, 247.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1885) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686706 reads in 194.770 CPU sec, 6.306 real sec [M::mem_process_seqs] Processed 685764 reads in 133.770 CPU sec, 4.282 real sec [M::process] read 684828 sequences (100000252 bp) in 3.058 CPU sec, 3.248 real sec... sorting block 20, #reads = 1083044 mem = 536877442 peak = 901812224 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 282720, 16, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 258, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (286.45, 122.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1159) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 305, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (322.51, 154.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (294, 486, 541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035) [M::mem_pestat] mean and std.dev: (356.86, 145.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1282) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 323, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (329.00, 164.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684778 sequences (100000239 bp) in 2.969 CPU sec, 3.238 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 282934, 8, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 238, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (261.39, 112.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 306, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (323.59, 154.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 258, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (290.97, 137.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685298 reads in 193.044 CPU sec, 6.224 real sec [M::mem_process_seqs] Processed 684828 reads in 131.903 CPU sec, 4.221 real sec sorting block 21, #reads = 1082171 mem = 536884809 peak = 901812224 [M::process] read 684336 sequences (100000141 bp) in 3.015 CPU sec, 3.172 real sec... [M::process] read 684198 sequences (100000180 bp) in 2.834 CPU sec, 3.091 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282233, 6, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 266, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (254.21, 117.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 310, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (326.55, 155.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 274, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (263.33, 96.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684336 reads in 105.771 CPU sec, 3.351 real sec sorting block 22, #reads = 1080690 mem = 536878041 peak = 901812224 [M::process] read 684370 sequences (100000159 bp) in 2.745 CPU sec, 2.893 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 282579, 10, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 361, 565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1221) [M::mem_pestat] mean and std.dev: (372.31, 196.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 308, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (325.10, 155.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (995, 995, 2661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5993) [M::mem_pestat] mean and std.dev: (1375.30, 904.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7659) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 298, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (293.93, 123.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282664, 7, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 264, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (284.29, 143.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 313, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (329.70, 157.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 369, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054) [M::mem_pestat] mean and std.dev: (338.97, 176.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1331) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684778 reads in 289.631 CPU sec, 9.262 real sec [M::mem_process_seqs] Processed 684198 reads in 147.077 CPU sec, 4.657 real sec sorting block 23, #reads = 1080711 mem = 536879637 peak = 901812224 [M::process] read 683928 sequences (100000138 bp) in 2.826 CPU sec, 3.000 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 282810, 0, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 324, 1435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3803) [M::mem_pestat] mean and std.dev: (640.08, 749.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4987) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 313, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (329.11, 157.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 356, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (285.55, 127.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1057) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683828 sequences (100000032 bp) in 3.169 CPU sec, 3.348 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 283130, 5, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 349, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (276.26, 95.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 315, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (331.60, 158.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 322, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974) [M::mem_pestat] mean and std.dev: (297.71, 139.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1218) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684370 reads in 181.946 CPU sec, 5.814 real sec sorting block 24, #reads = 1080683 mem = 536877560 peak = 901812224 [M::mem_process_seqs] Processed 683928 reads in 125.119 CPU sec, 3.962 real sec [M::process] read 683572 sequences (100000074 bp) in 2.968 CPU sec, 3.136 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 282824, 1, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 338, 631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1367) [M::mem_pestat] mean and std.dev: (389.27, 254.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1735) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 316, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (331.78, 158.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 308, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033) [M::mem_pestat] mean and std.dev: (346.62, 174.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1301) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 25, #reads = 1080601 mem = 536879604 peak = 901812224 [M::process] read 683250 sequences (100000271 bp) in 3.017 CPU sec, 3.157 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 282466, 3, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 315, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (330.78, 154.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 317, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (333.79, 159.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 346, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1140) [M::mem_pestat] mean and std.dev: (387.34, 208.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1445) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683828 reads in 180.439 CPU sec, 5.755 real sec [M::mem_process_seqs] Processed 683572 reads in 122.735 CPU sec, 3.880 real sec [M::process] read 682934 sequences (100000225 bp) in 2.945 CPU sec, 3.090 real sec... sorting block 26, #reads = 1080059 mem = 536878703 peak = 901812224 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 281928, 4, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 264, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (251.16, 84.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 318, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (334.37, 159.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1143) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 203, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (217.58, 82.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683090 sequences (100000121 bp) in 3.159 CPU sec, 3.358 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282959, 9, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 286, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (300.03, 142.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1162) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 318, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (333.67, 157.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 266, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (58, 473) [M::mem_pestat] mean and std.dev: (239.51, 62.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683250 reads in 177.447 CPU sec, 5.650 real sec [M::mem_process_seqs] Processed 682934 reads in 120.501 CPU sec, 3.820 real sec [M::process] read 682546 sequences (100000282 bp) in 2.556 CPU sec, 2.660 real sec... sorting block 27, #reads = 1079798 mem = 536881422 peak = 901812224 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 282538, 5, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 331, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (262.72, 124.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 321, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (336.31, 159.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 242, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971) [M::mem_pestat] mean and std.dev: (286.42, 148.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1238) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682900 sequences (100000097 bp) in 3.148 CPU sec, 3.270 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 282379, 15, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 320, 850) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2250) [M::mem_pestat] mean and std.dev: (465.00, 388.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2950) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 318, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (334.28, 157.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (495, 495, 611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (263, 843) [M::mem_pestat] mean and std.dev: (531.21, 81.73) [M::mem_pestat] low and high boundaries for proper pairs: (147, 959) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 307, 654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1644) [M::mem_pestat] mean and std.dev: (409.91, 381.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2139) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683090 reads in 177.244 CPU sec, 5.648 real sec [M::mem_process_seqs] Processed 682546 reads in 119.755 CPU sec, 3.778 real sec sorting block 28, #reads = 1080242 mem = 536883899 peak = 901812224 [M::process] read 683068 sequences (100000237 bp) in 2.957 CPU sec, 3.106 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282072, 6, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 262, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (262.81, 128.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1057) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 316, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (332.63, 157.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (135, 215, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837) [M::mem_pestat] mean and std.dev: (248.26, 117.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1071) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682654 sequences (100000058 bp) in 2.760 CPU sec, 2.894 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282260, 6, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 223, 550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1338) [M::mem_pestat] mean and std.dev: (305.14, 257.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1732) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 318, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (334.17, 158.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 315, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937) [M::mem_pestat] mean and std.dev: (291.80, 122.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1187) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682900 reads in 176.965 CPU sec, 5.625 real sec sorting block 29, #reads = 1078772 mem = 536880596 peak = 901812224 [M::mem_process_seqs] Processed 683068 reads in 121.333 CPU sec, 3.825 real sec [M::process] read 682396 sequences (100000155 bp) in 2.543 CPU sec, 2.630 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282261, 4, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 212, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (251.56, 109.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 318, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (334.29, 157.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 271, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088) [M::mem_pestat] mean and std.dev: (300.68, 172.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1385) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 30, #reads = 1079089 mem = 536877720 peak = 901812224 [M::process] read 683016 sequences (100000031 bp) in 3.006 CPU sec, 3.163 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 282431, 13, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 192, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (201.77, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 320, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (335.69, 158.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (995, 1308, 4598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11804) [M::mem_pestat] mean and std.dev: (1949.38, 1925.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15407) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 252, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (286.43, 157.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682654 reads in 177.180 CPU sec, 5.631 real sec [M::mem_process_seqs] Processed 682396 reads in 118.219 CPU sec, 3.727 real sec [M::process] read 683494 sequences (100000119 bp) in 2.807 CPU sec, 2.871 real sec... sorting block 31, #reads = 1079676 mem = 536876820 peak = 901812224 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 282119, 14, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 260, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (261.37, 110.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 316, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (333.72, 158.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1142) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (79, 476, 848) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2386) [M::mem_pestat] mean and std.dev: (546.64, 465.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3155) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 368, 927) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2361) [M::mem_pestat] mean and std.dev: (454.18, 394.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3078) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682378 sequences (100000038 bp) in 2.738 CPU sec, 2.870 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 282556, 5, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 292, 572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1364) [M::mem_pestat] mean and std.dev: (314.26, 162.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 312, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (330.80, 158.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 318, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060) [M::mem_pestat] mean and std.dev: (332.00, 180.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1343) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683016 reads in 178.373 CPU sec, 5.671 real sec [M::process] read 682974 sequences (100000051 bp) in 1.864 CPU sec, 1.876 real sec... sorting block 32, #reads = 1077311 mem = 536880477 peak = 901812224 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 281928, 6, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 271, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (231.45, 73.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 318, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (334.93, 158.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 384, 613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1401) [M::mem_pestat] mean and std.dev: (387.98, 199.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1795) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682990 sequences (100000065 bp) in 2.395 CPU sec, 2.398 real sec... [M::mem_process_seqs] Processed 682378 reads in 103.045 CPU sec, 3.260 real sec [M::mem_process_seqs] Processed 683494 reads in 256.663 CPU sec, 8.132 real sec sorting block 33, #reads = 1077834 mem = 536877390 peak = 901812224 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 282378, 16, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 345, 577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1299) [M::mem_pestat] mean and std.dev: (439.14, 321.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 314, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (332.15, 158.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (781, 1145, 1383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2587) [M::mem_pestat] mean and std.dev: (947.38, 461.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3189) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 266, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936) [M::mem_pestat] mean and std.dev: (312.37, 169.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1171) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682840 sequences (100000085 bp) in 2.856 CPU sec, 3.015 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281902, 10, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 367, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970) [M::mem_pestat] mean and std.dev: (304.92, 124.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1234) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 314, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (331.77, 158.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (122, 1278, 1278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3590) [M::mem_pestat] mean and std.dev: (978.89, 975.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4879) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 256, 643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1555) [M::mem_pestat] mean and std.dev: (292.30, 192.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2011) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682974 reads in 205.861 CPU sec, 6.521 real sec [M::mem_process_seqs] Processed 682990 reads in 130.497 CPU sec, 4.117 real sec [M::process] read 682732 sequences (100000147 bp) in 2.467 CPU sec, 2.517 real sec... sorting block 34, #reads = 1078671 mem = 536877983 peak = 901812224 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 281799, 0, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 216, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1209) [M::mem_pestat] mean and std.dev: (270.06, 166.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 315, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (332.43, 158.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 307, 667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1559) [M::mem_pestat] mean and std.dev: (491.24, 373.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2005) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682888 sequences (100000129 bp) in 2.705 CPU sec, 2.867 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282237, 12, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 181, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (177.43, 49.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 313, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (331.70, 157.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (2419, 2419, 4488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8626) [M::mem_pestat] mean and std.dev: (2303.80, 1392.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10695) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 293, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (331.71, 165.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1159) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682840 reads in 179.279 CPU sec, 5.695 real sec [M::process] read 682606 sequences (100000124 bp) in 2.265 CPU sec, 2.274 real sec... [M::mem_process_seqs] Processed 682732 reads in 121.475 CPU sec, 3.849 real sec sorting block 35, #reads = 1077723 mem = 536878634 peak = 901812224 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282360, 6, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 498, 893) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2277) [M::mem_pestat] mean and std.dev: (595.36, 501.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2969) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 315, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (333.56, 159.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1142) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (258, 384, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041) [M::mem_pestat] mean and std.dev: (376.57, 180.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1302) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682958 sequences (100000198 bp) in 2.700 CPU sec, 2.817 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 282277, 10, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 323, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1212) [M::mem_pestat] mean and std.dev: (384.14, 346.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 319, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (337.05, 161.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (423, 564, 1363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3243) [M::mem_pestat] mean and std.dev: (789.11, 741.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4183) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 389, 965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2467) [M::mem_pestat] mean and std.dev: (594.12, 553.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3218) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682422 sequences (100000072 bp) in 1.780 CPU sec, 1.777 real sec... [M::mem_process_seqs] Processed 682888 reads in 178.316 CPU sec, 5.674 real sec sorting block 36, #reads = 1078443 mem = 536878208 peak = 901812224 [M::mem_process_seqs] Processed 682606 reads in 121.383 CPU sec, 3.850 real sec sorting block 37, #reads = 1077118 mem = 536877844 peak = 901812224 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 282189, 4, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 261, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (281.03, 137.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1193) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 319, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (337.29, 163.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1163) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 250, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (294.72, 131.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1196) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685796 sequences (100000124 bp) in 3.118 CPU sec, 3.251 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282341, 7, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 216, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (238.00, 137.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 323, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932) [M::mem_pestat] mean and std.dev: (340.64, 164.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1173) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 330, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1198) [M::mem_pestat] mean and std.dev: (335.38, 196.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1528) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682958 reads in 176.284 CPU sec, 5.593 real sec [M::process] read 686786 sequences (100000207 bp) in 2.068 CPU sec, 2.091 real sec... [M::mem_process_seqs] Processed 682422 reads in 119.785 CPU sec, 3.781 real sec sorting block 38, #reads = 1077436 mem = 536879779 peak = 901812224 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 283380, 6, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (222.00, 83.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 303, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (322.97, 157.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 242, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (285.93, 150.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685798 sequences (100000254 bp) in 3.035 CPU sec, 3.169 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 283530, 9, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 207, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (237.71, 120.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 299, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (317.97, 154.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 289, 617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1497) [M::mem_pestat] mean and std.dev: (406.90, 289.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1937) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685796 reads in 174.079 CPU sec, 5.536 real sec [M::mem_process_seqs] Processed 686786 reads in 118.249 CPU sec, 3.724 real sec [M::process] read 685328 sequences (100000282 bp) in 2.702 CPU sec, 2.808 real sec... sorting block 39, #reads = 1078476 mem = 536880066 peak = 901812224 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 283432, 7, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (258, 308, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (96, 501) [M::mem_pestat] mean and std.dev: (276.19, 77.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 302, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (320.39, 154.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1100) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 355, 700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1640) [M::mem_pestat] mean and std.dev: (449.17, 292.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2110) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684828 sequences (100000068 bp) in 3.092 CPU sec, 3.237 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 283059, 9, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 275, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (245.14, 121.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1193) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 305, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (322.81, 155.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 278, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028) [M::mem_pestat] mean and std.dev: (302.02, 150.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1317) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685798 reads in 171.792 CPU sec, 5.460 real sec [M::mem_process_seqs] Processed 685328 reads in 116.480 CPU sec, 3.676 real sec sorting block 40, #reads = 1081290 mem = 536884963 peak = 901812224 [M::process] read 684402 sequences (100000204 bp) in 2.493 CPU sec, 2.574 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282969, 5, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 290, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962) [M::mem_pestat] mean and std.dev: (314.32, 229.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1233) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 306, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (323.81, 155.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1104) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (278, 327, 625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1319) [M::mem_pestat] mean and std.dev: (363.29, 148.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1666) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 41, #reads = 1080715 mem = 536881508 peak = 901812224 [M::process] read 684038 sequences (100000293 bp) in 3.248 CPU sec, 3.485 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 282998, 7, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 210, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976) [M::mem_pestat] mean and std.dev: (237.02, 172.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1263) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 309, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (325.78, 155.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 294, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (245.60, 76.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684828 reads in 169.664 CPU sec, 5.414 real sec [M::mem_process_seqs] Processed 684402 reads in 114.015 CPU sec, 3.605 real sec [M::process] read 684138 sequences (100000167 bp) in 2.879 CPU sec, 3.014 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282679, 4, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 268, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041) [M::mem_pestat] mean and std.dev: (301.80, 202.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1328) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 312, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (328.90, 157.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1123) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 270, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (266.29, 86.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 42, #reads = 1080916 mem = 536879520 peak = 901812224 [M::process] read 683878 sequences (100000132 bp) in 2.951 CPU sec, 3.047 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 282798, 8, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 307, 542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1156) [M::mem_pestat] mean and std.dev: (361.56, 263.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 313, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (329.91, 157.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 271, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (273.14, 115.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1007) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684038 reads in 170.483 CPU sec, 5.421 real sec [M::mem_process_seqs] Processed 684138 reads in 113.048 CPU sec, 3.565 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282571, 0, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 299, 790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1948) [M::mem_pestat] mean and std.dev: (370.31, 243.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2527) [M::mem_pestat] analyzing insert size distribution for orientation FR... sorting block 43, #reads = 1080079 mem = 536879215 peak = 901812224 [M::mem_pestat] (25, 50, 75) percentile: (205, 315, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (331.51, 158.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (250, 329, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991) [M::mem_pestat] mean and std.dev: (388.66, 227.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1299) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683982 sequences (100000128 bp) in 3.577 CPU sec, 3.843 real sec... [M::mem_process_seqs] Processed 683878 reads in 95.478 CPU sec, 3.170 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 282675, 3, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (253, 354, 602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1300) [M::mem_pestat] mean and std.dev: (348.10, 156.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 315, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (332.74, 160.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 314, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972) [M::mem_pestat] mean and std.dev: (334.33, 152.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1230) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683536 sequences (100000218 bp) in 2.980 CPU sec, 3.182 real sec... [M::mem_process_seqs] Processed 683982 reads in 93.578 CPU sec, 3.076 real sec sorting block 44, #reads = 1078593 mem = 536879095 peak = 901812224 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 283139, 1, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 290, 577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1403) [M::mem_pestat] mean and std.dev: (342.19, 222.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1816) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 319, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (336.20, 161.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1158) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 298, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (277.36, 144.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683264 sequences (100000219 bp) in 2.810 CPU sec, 3.042 real sec... [M::mem_process_seqs] Processed 683536 reads in 93.826 CPU sec, 3.097 real sec sorting block 45, #reads = 1079736 mem = 536878932 peak = 901812224 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 282342, 9, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 262, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076) [M::mem_pestat] mean and std.dev: (334.47, 175.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1367) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 682798 sequences (100000279 bp) in 2.946 CPU sec, 3.145 real sec... [M::mem_pestat] (25, 50, 75) percentile: (207, 319, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (335.63, 160.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1151) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 272, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (314.59, 152.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682710 sequences (100000215 bp) in 1.938 CPU sec, 1.961 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 281951, 7, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 265, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (41, 531) [M::mem_pestat] mean and std.dev: (262.14, 72.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 320, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (336.51, 160.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 307, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (296.18, 108.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683264 reads in 171.680 CPU sec, 5.492 real sec [M::mem_process_seqs] Processed 682798 reads in 114.214 CPU sec, 3.611 real sec [M::process] read 682528 sequences (100000145 bp) in 2.278 CPU sec, 2.371 real sec... sorting block 46, #reads = 1079483 mem = 536880925 peak = 901828608 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282200, 13, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 355, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1126) [M::mem_pestat] mean and std.dev: (301.86, 170.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 320, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (336.31, 159.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (78, 188, 1227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3525) [M::mem_pestat] mean and std.dev: (346.09, 362.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4674) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 460, 1100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2954) [M::mem_pestat] mean and std.dev: (628.25, 525.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3881) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682300 sequences (100000017 bp) in 2.970 CPU sec, 3.115 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 282045, 12, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 269, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1069) [M::mem_pestat] mean and std.dev: (292.95, 129.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 320, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (337.12, 160.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (690, 1321, 2714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6762) [M::mem_pestat] mean and std.dev: (1799.25, 1412.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8786) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 262, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (274.25, 137.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682710 reads in 175.276 CPU sec, 5.571 real sec [M::mem_process_seqs] Processed 682528 reads in 120.628 CPU sec, 3.806 real sec sorting block 47, #reads = 1079720 mem = 536878574 peak = 901828608 [M::process] read 682252 sequences (100000289 bp) in 2.976 CPU sec, 3.118 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 282149, 7, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 322, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1210) [M::mem_pestat] mean and std.dev: (311.21, 177.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 323, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (339.58, 161.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1155) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 281, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (290.05, 136.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1162) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 48, #reads = 1079980 mem = 536879811 peak = 901828608 [M::process] read 682512 sequences (100000216 bp) in 2.446 CPU sec, 2.547 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 281509, 2, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (240, 360, 598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1314) [M::mem_pestat] mean and std.dev: (400.62, 210.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 321, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (337.68, 160.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1150) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 298, 987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2623) [M::mem_pestat] mean and std.dev: (597.10, 633.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3441) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682300 reads in 172.835 CPU sec, 5.508 real sec [M::mem_process_seqs] Processed 682252 reads in 115.940 CPU sec, 3.653 real sec [M::process] read 682706 sequences (100000014 bp) in 2.729 CPU sec, 2.834 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 282338, 6, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 252, 616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1474) [M::mem_pestat] mean and std.dev: (364.10, 299.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1903) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 316, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (333.16, 157.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 278, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (261.25, 108.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 49, #reads = 1079269 mem = 536878615 peak = 901828608 [M::process] read 682492 sequences (100000276 bp) in 3.162 CPU sec, 3.393 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 282469, 5, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 222, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021) [M::mem_pestat] mean and std.dev: (271.14, 185.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1310) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 317, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (334.81, 159.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1143) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 291, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006) [M::mem_pestat] mean and std.dev: (317.00, 226.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1283) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682512 reads in 168.480 CPU sec, 5.377 real sec [M::mem_process_seqs] Processed 682706 reads in 113.773 CPU sec, 3.594 real sec [M::process] read 682696 sequences (100000157 bp) in 3.396 CPU sec, 3.603 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 282260, 12, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 303, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1229) [M::mem_pestat] mean and std.dev: (284.32, 158.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 316, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (333.82, 159.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (614, 9100, 9100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 26072) [M::mem_pestat] mean and std.dev: (4850.42, 4258.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 34558) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 279, 650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1558) [M::mem_pestat] mean and std.dev: (474.83, 403.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2091) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 50, #reads = 1078471 mem = 536879517 peak = 901828608 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 282606, 11, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 246, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (258.32, 155.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1083) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 315, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (332.87, 158.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (985, 1321, 1321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (313, 1993) [M::mem_pestat] mean and std.dev: (1075.50, 269.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2329) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 361, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011) [M::mem_pestat] mean and std.dev: (336.53, 176.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1281) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683312 sequences (100000037 bp) in 2.694 CPU sec, 2.821 real sec... [M::mem_process_seqs] Processed 682492 reads in 172.952 CPU sec, 5.537 real sec [M::mem_process_seqs] Processed 682696 reads in 119.516 CPU sec, 3.782 real sec sorting block 51, #reads = 1078930 mem = 536879254 peak = 912633856 [M::process] read 682910 sequences (100000125 bp) in 2.791 CPU sec, 3.069 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282565, 4, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 209, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (190.90, 60.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 312, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (331.25, 158.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (280, 326, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787) [M::mem_pestat] mean and std.dev: (336.38, 130.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282768, 14, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 279, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (242.84, 96.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 312, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (330.30, 157.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (422, 478, 1508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3680) [M::mem_pestat] mean and std.dev: (809.71, 545.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4766) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 288, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (263.14, 92.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683056 sequences (100000141 bp) in 3.318 CPU sec, 3.561 real sec... [M::mem_process_seqs] Processed 683312 reads in 173.535 CPU sec, 5.569 real sec [M::mem_process_seqs] Processed 682910 reads in 112.320 CPU sec, 3.569 real sec sorting block 52, #reads = 1079040 mem = 536878323 peak = 912633856 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282198, 10, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 299, 658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1580) [M::mem_pestat] mean and std.dev: (401.03, 319.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2041) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 312, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (330.16, 157.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (625, 878, 1635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3655) [M::mem_pestat] mean and std.dev: (1200.10, 578.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4665) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 246, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (221.65, 74.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683056 reads in 99.827 CPU sec, 3.298 real sec [M::process] read 682818 sequences (100000256 bp) in 3.650 CPU sec, 3.966 real sec... sorting block 53, #reads = 1078058 mem = 536880539 peak = 912633856 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 282010, 11, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 232, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021) [M::mem_pestat] mean and std.dev: (280.42, 157.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1308) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 311, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (329.89, 157.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1052, 1052, 1495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 2381) [M::mem_pestat] mean and std.dev: (1218.33, 398.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2824) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 361, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006) [M::mem_pestat] mean and std.dev: (351.47, 159.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1261) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682818 reads in 97.055 CPU sec, 3.191 real sec [M::process] read 683128 sequences (100000008 bp) in 3.625 CPU sec, 3.951 real sec... sorting block 54, #reads = 1078489 mem = 536878935 peak = 912633856 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 281764, 10, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 182, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (263.96, 211.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 311, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (328.47, 156.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (332, 332, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (369.70, 220.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1251) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 356, 609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1413) [M::mem_pestat] mean and std.dev: (348.22, 176.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1815) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683128 reads in 100.788 CPU sec, 3.264 real sec [M::process] read 683000 sequences (100000046 bp) in 3.096 CPU sec, 3.273 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282108, 10, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 194, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1183) [M::mem_pestat] mean and std.dev: (282.78, 263.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 312, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (330.97, 158.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (402, 406, 725) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1371) [M::mem_pestat] mean and std.dev: (401.12, 188.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1694) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 182, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (209.60, 79.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 55, #reads = 1078598 mem = 536880667 peak = 912633856 [M::mem_process_seqs] Processed 683000 reads in 101.558 CPU sec, 3.291 real sec [M::process] read 682730 sequences (100000077 bp) in 3.046 CPU sec, 3.322 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282730, 4, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 218, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1078) [M::mem_pestat] mean and std.dev: (266.09, 147.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1384) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 315, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (333.30, 160.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 281, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038) [M::mem_pestat] mean and std.dev: (307.22, 159.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1330) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682408 sequences (100000123 bp) in 2.830 CPU sec, 2.937 real sec... [M::mem_process_seqs] Processed 682730 reads in 100.119 CPU sec, 3.233 real sec sorting block 56, #reads = 1078141 mem = 536880778 peak = 912633856 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 282108, 6, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 245, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (247.26, 133.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 318, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (336.71, 161.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 320, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (314.12, 149.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682610 sequences (100000054 bp) in 3.211 CPU sec, 3.404 real sec... [M::mem_process_seqs] Processed 682408 reads in 99.081 CPU sec, 3.193 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 283209, 11, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 723) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1739) [M::mem_pestat] mean and std.dev: (397.70, 347.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2247) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 319, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (337.94, 162.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (736, 966, 1218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2182) [M::mem_pestat] mean and std.dev: (921.80, 478.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2836) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 230, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044) [M::mem_pestat] mean and std.dev: (298.35, 201.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1335) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682610 reads in 97.948 CPU sec, 3.169 real sec sorting block 57, #reads = 1077719 mem = 536880755 peak = 912633856 [M::process] read 682424 sequences (100000277 bp) in 3.377 CPU sec, 3.617 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282418, 17, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 237, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (282.36, 153.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 321, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932) [M::mem_pestat] mean and std.dev: (340.72, 165.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1173) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (102, 316, 2280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6636) [M::mem_pestat] mean and std.dev: (908.88, 1162.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8814) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 336, 605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1435) [M::mem_pestat] mean and std.dev: (423.97, 320.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1850) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682172 sequences (100000090 bp) in 2.751 CPU sec, 2.836 real sec... [M::mem_process_seqs] Processed 682424 reads in 96.391 CPU sec, 3.095 real sec sorting block 58, #reads = 1078896 mem = 536879898 peak = 912633856 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282469, 13, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 293, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (284.32, 106.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 325, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945) [M::mem_pestat] mean and std.dev: (344.04, 166.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1190) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (115, 970, 2580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7510) [M::mem_pestat] mean and std.dev: (1134.38, 1059.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9975) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (285, 343, 593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1209) [M::mem_pestat] mean and std.dev: (435.36, 286.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1580) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686648 sequences (100000237 bp) in 2.838 CPU sec, 2.952 real sec... [M::mem_process_seqs] Processed 682172 reads in 103.718 CPU sec, 3.339 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 283444, 7, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 220, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (236.43, 101.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 299, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (319.51, 156.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (247, 510, 1029) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2593) [M::mem_pestat] mean and std.dev: (576.00, 492.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3375) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685932 sequences (100000160 bp) in 3.264 CPU sec, 3.480 real sec... [M::mem_process_seqs] Processed 686648 reads in 105.867 CPU sec, 3.417 real sec sorting block 59, #reads = 1078219 mem = 536878362 peak = 912633856 [M::process] read 685582 sequences (100000063 bp) in 2.858 CPU sec, 2.970 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 283338, 5, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (257, 408, 763) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1775) [M::mem_pestat] mean and std.dev: (401.41, 242.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2281) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 302, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (322.63, 157.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 292, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025) [M::mem_pestat] mean and std.dev: (320.05, 178.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1309) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684800 sequences (100000108 bp) in 2.040 CPU sec, 2.034 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 283218, 4, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 322, 649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1555) [M::mem_pestat] mean and std.dev: (369.98, 246.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2008) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 306, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (325.03, 158.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 289, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1104) [M::mem_pestat] mean and std.dev: (356.68, 213.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1405) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685932 reads in 177.141 CPU sec, 5.570 real sec [M::mem_process_seqs] Processed 685582 reads in 118.780 CPU sec, 3.732 real sec [M::process] read 684324 sequences (100000063 bp) in 2.776 CPU sec, 2.929 real sec... sorting block 60, #reads = 1082867 mem = 536885037 peak = 912633856 [M::process] read 683948 sequences (100000042 bp) in 3.081 CPU sec, 3.338 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282953, 1, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 190, 577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1411) [M::mem_pestat] mean and std.dev: (296.35, 251.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1828) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 312, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (330.14, 159.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 322, 827) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2185) [M::mem_pestat] mean and std.dev: (427.58, 422.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2864) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684324 reads in 100.136 CPU sec, 3.167 real sec sorting block 61, #reads = 1082678 mem = 536881531 peak = 912633856 [M::process] read 684140 sequences (100000048 bp) in 1.856 CPU sec, 1.912 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282997, 9, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 246, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (244.47, 79.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 310, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (329.37, 160.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 271, 575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1349) [M::mem_pestat] mean and std.dev: (434.82, 357.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1866) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 283168, 2, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 306, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784) [M::mem_pestat] mean and std.dev: (298.39, 153.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 312, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (329.32, 157.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (278, 369, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1073) [M::mem_pestat] mean and std.dev: (418.43, 221.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1338) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684800 reads in 273.121 CPU sec, 8.690 real sec [M::mem_process_seqs] Processed 683948 reads in 138.395 CPU sec, 4.379 real sec [M::process] read 683526 sequences (100000280 bp) in 2.482 CPU sec, 2.524 real sec... sorting block 62, #reads = 1081455 mem = 536879249 peak = 958717952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 283316, 5, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 309, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1206) [M::mem_pestat] mean and std.dev: (318.11, 197.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 314, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (331.39, 159.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 281, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (297.82, 192.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683596 sequences (100000208 bp) in 3.374 CPU sec, 3.587 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282274, 9, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 241, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (208.85, 56.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 317, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (335.26, 161.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 261, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (289.12, 146.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684140 reads in 173.684 CPU sec, 5.516 real sec [M::mem_process_seqs] Processed 683526 reads in 116.855 CPU sec, 3.689 real sec sorting block 63, #reads = 1082408 mem = 536880047 peak = 958717952 [M::process] read 683208 sequences (100000167 bp) in 2.594 CPU sec, 2.666 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282645, 4, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 215, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (14, 419) [M::mem_pestat] mean and std.dev: (204.91, 58.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 317, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923) [M::mem_pestat] mean and std.dev: (335.82, 161.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1162) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 369, 639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1487) [M::mem_pestat] mean and std.dev: (349.28, 226.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1911) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682960 sequences (100000001 bp) in 2.674 CPU sec, 2.767 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282634, 9, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 246, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (313.55, 173.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 321, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (338.35, 162.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1171) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 277, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (289.80, 136.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1078) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683596 reads in 178.144 CPU sec, 5.673 real sec sorting block 64, #reads = 1080376 mem = 536880387 peak = 958717952 [M::process] read 682862 sequences (100000193 bp) in 2.319 CPU sec, 2.354 real sec... [M::mem_process_seqs] Processed 683208 reads in 119.997 CPU sec, 3.786 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 282651, 7, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 269, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (267.21, 98.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 322, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931) [M::mem_pestat] mean and std.dev: (339.14, 163.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 273, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (255.14, 78.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 65, #reads = 1079878 mem = 536878948 peak = 958717952 [M::process] read 682744 sequences (100000216 bp) in 2.936 CPU sec, 3.138 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 282286, 11, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 212, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (199.47, 96.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 323, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932) [M::mem_pestat] mean and std.dev: (340.18, 163.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1173) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (398, 491, 855) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1769) [M::mem_pestat] mean and std.dev: (485.56, 173.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2226) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 353, 554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1208) [M::mem_pestat] mean and std.dev: (445.39, 289.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1601) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682960 reads in 171.044 CPU sec, 5.455 real sec [M::process] read 682900 sequences (100000105 bp) in 1.929 CPU sec, 1.957 real sec... [M::mem_process_seqs] Processed 682862 reads in 115.874 CPU sec, 3.664 real sec sorting block 66, #reads = 1081034 mem = 536880106 peak = 958717952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282944, 6, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 279, 572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1396) [M::mem_pestat] mean and std.dev: (273.74, 156.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 323, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (339.34, 161.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1166) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 232, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (226.00, 102.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1017) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682558 sequences (100000102 bp) in 3.044 CPU sec, 3.248 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 281983, 4, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 392, 825) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2063) [M::mem_pestat] mean and std.dev: (491.97, 350.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 321, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (337.67, 161.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 214, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990) [M::mem_pestat] mean and std.dev: (304.98, 154.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1260) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682744 reads in 175.625 CPU sec, 5.589 real sec [M::mem_process_seqs] Processed 682900 reads in 122.371 CPU sec, 3.854 real sec [M::process] read 682520 sequences (100000107 bp) in 2.544 CPU sec, 2.614 real sec... sorting block 67, #reads = 1080296 mem = 536880037 peak = 958717952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281964, 0, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 226, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (203.75, 73.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 321, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (337.91, 161.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (135, 281, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1167) [M::mem_pestat] mean and std.dev: (284.26, 171.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1511) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681770 sequences (100000291 bp) in 3.011 CPU sec, 3.123 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 282054, 9, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 235, 1279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3525) [M::mem_pestat] mean and std.dev: (617.69, 746.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 319, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (336.18, 159.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 222, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (208.54, 79.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682558 reads in 183.002 CPU sec, 5.818 real sec [M::mem_process_seqs] Processed 682520 reads in 123.580 CPU sec, 3.896 real sec [M::process] read 682554 sequences (100000296 bp) in 2.555 CPU sec, 2.627 real sec... sorting block 68, #reads = 1079429 mem = 536877944 peak = 958717952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 281709, 3, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 198, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (209.43, 103.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 322, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (338.47, 159.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (272, 331, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (350.83, 155.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1068) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 69, #reads = 1079576 mem = 536880225 peak = 992776192 [M::process] read 683302 sequences (100000049 bp) in 2.927 CPU sec, 3.053 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 281799, 6, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 287, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (265.61, 141.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 320, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (336.66, 160.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 254, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794) [M::mem_pestat] mean and std.dev: (256.58, 112.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1006) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681770 reads in 180.379 CPU sec, 5.743 real sec [M::mem_process_seqs] Processed 682554 reads in 121.182 CPU sec, 3.823 real sec [M::process] read 683250 sequences (100000032 bp) in 2.792 CPU sec, 2.904 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282093, 2, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 249, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (36, 416) [M::mem_pestat] mean and std.dev: (220.73, 59.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 311, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (329.08, 156.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (265, 337, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (349.82, 160.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 70, #reads = 1079638 mem = 536879291 peak = 992776192 [M::process] read 683018 sequences (100000036 bp) in 2.986 CPU sec, 3.099 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 281878, 21, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 242, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (224.88, 79.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 308, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (326.59, 155.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (755, 836, 2780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6830) [M::mem_pestat] mean and std.dev: (1540.75, 1701.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8855) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 250, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (263.07, 131.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683302 reads in 176.112 CPU sec, 5.606 real sec [M::mem_process_seqs] Processed 683250 reads in 119.459 CPU sec, 3.771 real sec [M::process] read 683226 sequences (100000003 bp) in 3.057 CPU sec, 3.178 real sec... sorting block 71, #reads = 1078546 mem = 536884666 peak = 992776192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 282129, 9, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 255, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1151) [M::mem_pestat] mean and std.dev: (299.20, 188.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 312, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (330.23, 157.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 266, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (289.07, 142.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1159) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683320 sequences (100000293 bp) in 2.923 CPU sec, 3.070 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 282284, 7, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 265, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017) [M::mem_pestat] mean and std.dev: (290.04, 144.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1301) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 312, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (330.25, 157.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 287, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1123) [M::mem_pestat] mean and std.dev: (302.84, 203.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1437) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683018 reads in 178.118 CPU sec, 5.684 real sec [M::mem_process_seqs] Processed 683226 reads in 119.237 CPU sec, 3.778 real sec sorting block 72, #reads = 1078005 mem = 536878499 peak = 992776192 [M::process] read 683014 sequences (100000024 bp) in 3.256 CPU sec, 3.451 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282976, 3, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 327, 788) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1976) [M::mem_pestat] mean and std.dev: (488.92, 431.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 310, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (328.70, 156.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 295, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1229) [M::mem_pestat] mean and std.dev: (336.79, 211.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1575) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 282174, 12, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 263, 764) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1918) [M::mem_pestat] mean and std.dev: (316.88, 290.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 309, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (327.01, 154.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1100) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (540, 705, 2384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6072) [M::mem_pestat] mean and std.dev: (1361.17, 947.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7916) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 246, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (253.87, 76.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683178 sequences (100000089 bp) in 3.355 CPU sec, 3.569 real sec... [M::mem_process_seqs] Processed 683320 reads in 174.938 CPU sec, 5.593 real sec sorting block 73, #reads = 1079387 mem = 536881254 peak = 992776192 [M::mem_process_seqs] Processed 683014 reads in 118.927 CPU sec, 3.767 real sec [M::process] read 683218 sequences (100000099 bp) in 3.078 CPU sec, 3.229 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 282137, 4, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 272, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042) [M::mem_pestat] mean and std.dev: (394.48, 273.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 309, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (327.72, 155.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 253, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (276.86, 118.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 74, #reads = 1078114 mem = 536880747 peak = 992776192 [M::process] read 682874 sequences (100000154 bp) in 2.698 CPU sec, 2.776 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 282283, 12, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 216, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (256.38, 127.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 992) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 313, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (331.21, 157.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (521, 1562, 2289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5825) [M::mem_pestat] mean and std.dev: (1435.33, 1047.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7593) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 298, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (282.22, 130.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683178 reads in 184.352 CPU sec, 5.868 real sec [M::mem_process_seqs] Processed 683218 reads in 125.074 CPU sec, 3.944 real sec [M::process] read 682938 sequences (100000161 bp) in 2.996 CPU sec, 3.130 real sec... sorting block 75, #reads = 1078026 mem = 536878492 peak = 992776192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 281967, 8, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 226, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (229.03, 153.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 315, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (332.47, 158.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 292, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020) [M::mem_pestat] mean and std.dev: (330.16, 142.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1284) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682794 sequences (100000013 bp) in 2.929 CPU sec, 3.033 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282220, 2, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 228, 742) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1960) [M::mem_pestat] mean and std.dev: (358.17, 406.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 313, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (330.56, 156.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 293, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (286.38, 120.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682874 reads in 183.361 CPU sec, 5.835 real sec [M::mem_process_seqs] Processed 682938 reads in 122.331 CPU sec, 3.862 real sec [M::process] read 681068 sequences (100000120 bp) in 2.646 CPU sec, 2.728 real sec... sorting block 76, #reads = 1078448 mem = 536878381 peak = 992776192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282687, 3, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 263, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956) [M::mem_pestat] mean and std.dev: (285.15, 183.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1225) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 317, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (335.20, 160.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 291, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (308.45, 147.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682468 sequences (100000193 bp) in 2.676 CPU sec, 2.931 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 282319, 7, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 267, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (282.22, 122.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 328, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (344.46, 162.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 246, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1220) [M::mem_pestat] mean and std.dev: (287.31, 169.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1570) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682794 reads in 175.540 CPU sec, 5.606 real sec [M::mem_process_seqs] Processed 681068 reads in 118.982 CPU sec, 3.763 real sec [M::process] read 682738 sequences (100000138 bp) in 2.000 CPU sec, 2.073 real sec... sorting block 77, #reads = 1078896 mem = 536879436 peak = 992776192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282210, 5, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (331, 402, 666) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1336) [M::mem_pestat] mean and std.dev: (396.28, 246.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 319, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (337.99, 162.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 274, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (284.03, 93.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 691118 sequences (100000300 bp) in 3.418 CPU sec, 3.593 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 282585, 0, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 289, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1121) [M::mem_pestat] mean and std.dev: (336.35, 180.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 320, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (338.91, 163.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1168) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (255, 325, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (328.57, 139.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682468 reads in 176.451 CPU sec, 5.623 real sec sorting block 78, #reads = 1077924 mem = 536879637 peak = 992776192 [M::mem_process_seqs] Processed 682738 reads in 119.155 CPU sec, 3.761 real sec [M::process] read 690366 sequences (100000135 bp) in 2.817 CPU sec, 2.901 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 285706, 13, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 224, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (211.10, 68.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 275, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (303.10, 154.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1083) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (372, 429, 2006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5274) [M::mem_pestat] mean and std.dev: (816.38, 906.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6908) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 311, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (346.00, 185.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1089) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 79, #reads = 1078143 mem = 536880442 peak = 992776192 [M::process] read 689180 sequences (100000069 bp) in 3.084 CPU sec, 3.263 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 285026, 19, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 329, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1134) [M::mem_pestat] mean and std.dev: (378.15, 260.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 280, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (306.53, 154.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (73, 98, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1297) [M::mem_pestat] mean and std.dev: (218.89, 252.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1705) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 352, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016) [M::mem_pestat] mean and std.dev: (377.97, 280.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1502) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691118 reads in 196.849 CPU sec, 6.259 real sec [M::mem_process_seqs] Processed 690366 reads in 133.783 CPU sec, 4.219 real sec [M::process] read 688590 sequences (100000069 bp) in 2.870 CPU sec, 2.989 real sec... sorting block 80, #reads = 1078901 mem = 536879094 peak = 992776192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 285384, 8, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 258, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028) [M::mem_pestat] mean and std.dev: (209.07, 97.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1325) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 288, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (312.23, 156.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 324, 547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1313) [M::mem_pestat] mean and std.dev: (331.16, 168.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1696) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687832 sequences (100000238 bp) in 3.425 CPU sec, 3.646 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 285194, 8, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 183, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1372) [M::mem_pestat] mean and std.dev: (280.69, 288.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 291, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (314.36, 156.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 249, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (249.47, 99.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 990) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689180 reads in 189.217 CPU sec, 6.033 real sec [M::mem_process_seqs] Processed 688590 reads in 125.612 CPU sec, 3.967 real sec sorting block 81, #reads = 1079954 mem = 536877979 peak = 992776192 [M::process] read 686250 sequences (100000020 bp) in 3.339 CPU sec, 3.547 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 284543, 5, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 177, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (195.44, 119.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 295, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (318.54, 159.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (137, 200, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1154) [M::mem_pestat] mean and std.dev: (302.92, 236.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1493) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686060 sequences (100000014 bp) in 2.741 CPU sec, 2.839 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 283926, 1, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 283, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (262.10, 111.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 300, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (321.93, 158.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (286, 404, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (350.87, 129.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1066) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687832 reads in 188.905 CPU sec, 6.025 real sec [M::mem_process_seqs] Processed 686250 reads in 123.542 CPU sec, 3.897 real sec sorting block 82, #reads = 1079763 mem = 536881866 peak = 992776192 [M::process] read 685218 sequences (100000217 bp) in 3.062 CPU sec, 3.214 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 283670, 19, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 358, 592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1384) [M::mem_pestat] mean and std.dev: (376.43, 215.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 305, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (326.58, 161.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1167) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (231, 2038, 5591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16311) [M::mem_pestat] mean and std.dev: (3152.37, 2908.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21671) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 334, 561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1285) [M::mem_pestat] mean and std.dev: (287.96, 130.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1647) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 83, #reads = 1079262 mem = 536880467 peak = 992776192 [M::process] read 684642 sequences (100000271 bp) in 3.274 CPU sec, 3.503 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 283334, 3, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 359, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024) [M::mem_pestat] mean and std.dev: (355.20, 204.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1301) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 311, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (331.59, 162.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1179) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 243, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (231.00, 68.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686060 reads in 183.599 CPU sec, 5.867 real sec [M::mem_process_seqs] Processed 685218 reads in 123.601 CPU sec, 3.911 real sec [M::process] read 685324 sequences (100000272 bp) in 3.557 CPU sec, 3.825 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 283336, 0, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 342, 540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1210) [M::mem_pestat] mean and std.dev: (366.06, 221.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 315, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945) [M::mem_pestat] mean and std.dev: (334.82, 163.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1193) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 268, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006) [M::mem_pestat] mean and std.dev: (285.48, 149.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1292) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 84, #reads = 1078957 mem = 536880994 peak = 992776192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 284083, 3, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 286, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (253.52, 105.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 312, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (331.02, 161.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1179) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (260, 282, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (351.75, 168.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1026) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685174 sequences (100000040 bp) in 2.992 CPU sec, 3.129 real sec... [M::mem_process_seqs] Processed 684642 reads in 181.740 CPU sec, 5.802 real sec [M::mem_process_seqs] Processed 685324 reads in 121.335 CPU sec, 3.835 real sec sorting block 85, #reads = 1078387 mem = 536880162 peak = 992776192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 283334, 4, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 257, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (313.22, 167.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 312, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (331.01, 160.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1179) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 254, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (289.31, 138.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685052 sequences (100000029 bp) in 3.871 CPU sec, 4.164 real sec... [M::mem_process_seqs] Processed 685174 reads in 108.337 CPU sec, 3.555 real sec sorting block 86, #reads = 1078384 mem = 536879336 peak = 992776192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 283945, 5, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 214, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (244.98, 119.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 311, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (329.42, 159.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1163) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 493, 844) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2128) [M::mem_pestat] mean and std.dev: (510.03, 355.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2770) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684926 sequences (100000289 bp) in 3.354 CPU sec, 3.576 real sec... [M::mem_process_seqs] Processed 685052 reads in 111.218 CPU sec, 3.607 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 283319, 9, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 233, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1178) [M::mem_pestat] mean and std.dev: (365.61, 296.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 313, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (330.81, 160.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1171) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 300, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (296.47, 128.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684988 sequences (100000172 bp) in 2.910 CPU sec, 3.046 real sec... [M::mem_process_seqs] Processed 684926 reads in 108.183 CPU sec, 3.467 real sec sorting block 87, #reads = 1074094 mem = 536881170 peak = 992776192 [M::process] read 684744 sequences (100000226 bp) in 3.016 CPU sec, 3.164 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 283260, 11, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 221, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1160) [M::mem_pestat] mean and std.dev: (311.22, 213.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 312, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (330.45, 159.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1168) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (649, 1745, 4951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13555) [M::mem_pestat] mean and std.dev: (2800.73, 2209.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17857) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (262, 379, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (311.56, 116.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685410 sequences (100000027 bp) in 2.280 CPU sec, 2.272 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 283050, 4, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 272, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (260.86, 136.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 313, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (330.61, 159.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1168) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 231, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (247.97, 128.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684988 reads in 187.452 CPU sec, 5.891 real sec [M::mem_process_seqs] Processed 684744 reads in 122.651 CPU sec, 3.857 real sec [M::process] read 684456 sequences (100000239 bp) in 2.404 CPU sec, 2.471 real sec... sorting block 88, #reads = 1076526 mem = 536882816 peak = 992776192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283292, 10, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 203, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (279.21, 170.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 307, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (327.13, 159.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (394, 4387, 4387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12373) [M::mem_pestat] mean and std.dev: (2526.90, 1927.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16366) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (266, 387, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (314.08, 135.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683842 sequences (100000101 bp) in 2.456 CPU sec, 2.569 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 282786, 7, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 318, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841) [M::mem_pestat] mean and std.dev: (260.76, 106.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 313, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932) [M::mem_pestat] mean and std.dev: (331.58, 160.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1176) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 281, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996) [M::mem_pestat] mean and std.dev: (325.61, 139.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1252) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684122 sequences (100000046 bp) in 1.740 CPU sec, 1.735 real sec... [M::mem_process_seqs] Processed 685410 reads in 188.202 CPU sec, 5.988 real sec sorting block 89, #reads = 1076829 mem = 536881197 peak = 992776192 [M::mem_process_seqs] Processed 684456 reads in 125.033 CPU sec, 3.968 real sec sorting block 90, #reads = 1076353 mem = 536879206 peak = 1066196992 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 282681, 12, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 297, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (280.29, 114.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 315, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923) [M::mem_pestat] mean and std.dev: (332.62, 158.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1163) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (568, 1567, 6774) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 19186) [M::mem_pestat] mean and std.dev: (3221.58, 3017.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 25392) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 339, 630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1444) [M::mem_pestat] mean and std.dev: (392.35, 270.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1851) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683426 sequences (100000042 bp) in 2.810 CPU sec, 2.889 real sec... [M::process] read 684112 sequences (100000121 bp) in 1.760 CPU sec, 1.758 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 283031, 14, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 278, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (320.88, 154.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 314, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (332.84, 160.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1170) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (619, 1008, 2149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5209) [M::mem_pestat] mean and std.dev: (1527.21, 1400.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7129) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 253, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (248.54, 85.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683842 reads in 184.675 CPU sec, 5.851 real sec [M::mem_process_seqs] Processed 684122 reads in 123.178 CPU sec, 3.920 real sec sorting block 91, #reads = 1076381 mem = 536878334 peak = 1066196992 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 282735, 11, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 275, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (276.41, 125.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 320, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943) [M::mem_pestat] mean and std.dev: (337.57, 162.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1189) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (979, 1584, 1584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2794) [M::mem_pestat] mean and std.dev: (1202.33, 456.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3399) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 310, 635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1513) [M::mem_pestat] mean and std.dev: (347.72, 192.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1952) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683952 sequences (100000243 bp) in 2.771 CPU sec, 2.995 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 283112, 5, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 209, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1165) [M::mem_pestat] mean and std.dev: (370.79, 316.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 313, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931) [M::mem_pestat] mean and std.dev: (332.88, 161.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 232, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (248.42, 101.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683530 sequences (100000043 bp) in 2.040 CPU sec, 2.035 real sec... [M::mem_process_seqs] Processed 683426 reads in 195.378 CPU sec, 6.208 real sec [M::mem_process_seqs] Processed 684112 reads in 131.775 CPU sec, 4.177 real sec sorting block 92, #reads = 1075890 mem = 536884176 peak = 1066196992 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 282914, 7, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 326, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (307.71, 131.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1204) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 313, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (332.51, 160.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1166) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 233, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (236.69, 83.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683046 sequences (100000087 bp) in 3.142 CPU sec, 3.220 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 282049, 2, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 189, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (187.12, 99.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 315, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (333.93, 160.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1168) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 234, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (305.31, 193.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683126 sequences (100000125 bp) in 2.370 CPU sec, 2.373 real sec... [M::mem_process_seqs] Processed 683952 reads in 194.247 CPU sec, 6.134 real sec sorting block 93, #reads = 1075393 mem = 536882579 peak = 1066196992 [M::mem_process_seqs] Processed 683530 reads in 128.124 CPU sec, 4.053 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 282299, 8, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 214, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (218.70, 105.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 318, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932) [M::mem_pestat] mean and std.dev: (336.65, 161.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (238, 314, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (280.62, 88.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683074 sequences (100000005 bp) in 2.373 CPU sec, 2.511 real sec... [M::process] read 682888 sequences (100000046 bp) in 2.150 CPU sec, 2.152 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 282598, 4, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 289, 592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1404) [M::mem_pestat] mean and std.dev: (351.06, 217.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1810) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 319, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (337.78, 162.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1182) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 313, 636) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1488) [M::mem_pestat] mean and std.dev: (380.94, 249.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1914) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683046 reads in 188.307 CPU sec, 5.980 real sec sorting block 94, #reads = 1075133 mem = 536879599 peak = 1066196992 [M::mem_process_seqs] Processed 683126 reads in 123.548 CPU sec, 3.919 real sec sorting block 95, #reads = 1074955 mem = 536877633 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 282374, 12, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (248, 483, 665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1499) [M::mem_pestat] mean and std.dev: (463.67, 255.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1916) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 319, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (337.94, 162.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1182) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (585, 713, 1135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2235) [M::mem_pestat] mean and std.dev: (833.67, 491.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2800) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 243, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (254.52, 106.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682866 sequences (100000008 bp) in 3.136 CPU sec, 3.201 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 282440, 8, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 229, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023) [M::mem_pestat] mean and std.dev: (344.97, 254.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 322, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951) [M::mem_pestat] mean and std.dev: (341.72, 166.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1199) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 183, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (198.28, 68.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683074 reads in 185.592 CPU sec, 5.855 real sec [M::process] read 691860 sequences (100000002 bp) in 2.530 CPU sec, 2.535 real sec... [M::mem_process_seqs] Processed 682888 reads in 124.395 CPU sec, 3.920 real sec sorting block 96, #reads = 1074566 mem = 536885283 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282113, 6, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 352, 569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1367) [M::mem_pestat] mean and std.dev: (359.46, 254.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1766) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 321, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948) [M::mem_pestat] mean and std.dev: (341.16, 165.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1195) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 232, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (247.40, 83.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692864 sequences (100000274 bp) in 2.860 CPU sec, 2.937 real sec... [M::process] read 690778 sequences (100000218 bp) in 1.970 CPU sec, 1.966 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 286077, 4, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 281, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1095) [M::mem_pestat] mean and std.dev: (305.90, 161.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 273, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849) [M::mem_pestat] mean and std.dev: (300.06, 152.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1073) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 284, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (291.43, 109.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682866 reads in 195.543 CPU sec, 6.199 real sec [M::mem_process_seqs] Processed 691860 reads in 133.950 CPU sec, 4.249 real sec sorting block 97, #reads = 1075182 mem = 536881069 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 286648, 3, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 250, 598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1442) [M::mem_pestat] mean and std.dev: (382.44, 295.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 269, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (294.00, 147.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 227, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (253.40, 120.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 690122 sequences (100000272 bp) in 3.351 CPU sec, 3.429 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 285694, 5, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 253, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (6, 561) [M::mem_pestat] mean and std.dev: (265.56, 101.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 279, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (302.41, 150.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1073) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 320, 635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1433) [M::mem_pestat] mean and std.dev: (430.30, 285.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1832) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692864 reads in 197.420 CPU sec, 6.246 real sec [M::process] read 689528 sequences (100000063 bp) in 2.561 CPU sec, 2.568 real sec... sorting block 98, #reads = 1074737 mem = 536883829 peak = 1092800512 [M::mem_process_seqs] Processed 690778 reads in 130.375 CPU sec, 4.099 real sec sorting block 99, #reads = 1076130 mem = 536880720 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 285455, 12, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (242, 360, 817) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1967) [M::mem_pestat] mean and std.dev: (437.03, 334.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 281, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (303.87, 149.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1074) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (626, 3005, 3005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7763) [M::mem_pestat] mean and std.dev: (1779.08, 1338.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10142) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 239, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1160) [M::mem_pestat] mean and std.dev: (265.38, 197.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1494) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689642 sequences (100000139 bp) in 2.981 CPU sec, 3.067 real sec... [M::process] read 688798 sequences (100000059 bp) in 1.910 CPU sec, 1.915 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 285140, 14, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 232, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (253.08, 95.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 285, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (305.35, 149.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1072) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (252, 775, 1141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2919) [M::mem_pestat] mean and std.dev: (807.93, 692.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3808) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 357, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1114) [M::mem_pestat] mean and std.dev: (328.52, 148.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1417) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690122 reads in 199.284 CPU sec, 6.321 real sec [M::mem_process_seqs] Processed 689528 reads in 132.336 CPU sec, 4.186 real sec sorting block 100, #reads = 1075339 mem = 536882476 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 285413, 8, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 242, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943) [M::mem_pestat] mean and std.dev: (248.62, 133.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1208) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 284, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (304.15, 147.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1068) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 316, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (269.73, 104.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688340 sequences (100000220 bp) in 3.663 CPU sec, 3.819 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 285277, 16, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 234, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016) [M::mem_pestat] mean and std.dev: (295.69, 235.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1301) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 290, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (309.50, 150.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1087) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (252, 558, 1440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3816) [M::mem_pestat] mean and std.dev: (650.43, 509.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5004) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 239, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (273.40, 124.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689642 reads in 199.102 CPU sec, 6.285 real sec [M::process] read 687276 sequences (100000235 bp) in 2.498 CPU sec, 2.492 real sec... [M::mem_process_seqs] Processed 688798 reads in 129.846 CPU sec, 4.094 real sec sorting block 101, #reads = 1072856 mem = 536881865 peak = 1092800512 [M::process] read 687150 sequences (100000071 bp) in 3.321 CPU sec, 3.495 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 284304, 15, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 238, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1058) [M::mem_pestat] mean and std.dev: (281.28, 165.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1356) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 296, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (315.62, 153.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (269, 495, 798) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1856) [M::mem_pestat] mean and std.dev: (384.08, 187.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2385) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 277, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062) [M::mem_pestat] mean and std.dev: (327.53, 225.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1358) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687276 reads in 104.267 CPU sec, 3.270 real sec [M::process] read 687518 sequences (100000032 bp) in 2.547 CPU sec, 2.559 real sec... sorting block 102, #reads = 1073660 mem = 536880590 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 284864, 6, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 253, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1064) [M::mem_pestat] mean and std.dev: (279.95, 205.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1371) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 291, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (310.44, 150.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1099) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 313, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (273.31, 111.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 284734, 10, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (262, 272, 1959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5353) [M::mem_pestat] mean and std.dev: (784.65, 755.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7050) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 298, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (317.64, 154.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (412, 880, 1012) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2212) [M::mem_pestat] mean and std.dev: (728.70, 486.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2812) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 310, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936) [M::mem_pestat] mean and std.dev: (282.29, 129.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1185) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688340 reads in 295.562 CPU sec, 9.333 real sec [M::process] read 686270 sequences (100000221 bp) in 2.812 CPU sec, 2.833 real sec... [M::mem_process_seqs] Processed 687150 reads in 149.416 CPU sec, 4.717 real sec sorting block 103, #reads = 1075461 mem = 536880990 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 284728, 8, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 262, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (282.46, 115.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 912) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 297, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (316.44, 154.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 392, 692) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1694) [M::mem_pestat] mean and std.dev: (403.17, 280.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2195) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686188 sequences (100000176 bp) in 2.637 CPU sec, 2.780 real sec... [M::process] read 687000 sequences (100000142 bp) in 1.860 CPU sec, 1.862 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 284324, 1, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 249, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (225.63, 93.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 302, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (320.09, 154.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 320, 499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051) [M::mem_pestat] mean and std.dev: (324.77, 180.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1327) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687518 reads in 193.219 CPU sec, 6.147 real sec [M::mem_process_seqs] Processed 686270 reads in 122.702 CPU sec, 3.897 real sec sorting block 104, #reads = 1075750 mem = 536877238 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 284038, 8, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 318, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (259.97, 109.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 304, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (322.69, 155.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 259, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1231) [M::mem_pestat] mean and std.dev: (344.27, 189.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1575) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686242 sequences (100000211 bp) in 2.744 CPU sec, 2.861 real sec... [M::process] read 685052 sequences (100000086 bp) in 1.917 CPU sec, 1.919 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 284619, 10, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 334, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954) [M::mem_pestat] mean and std.dev: (279.44, 136.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1214) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 298, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (318.84, 155.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (909, 9808, 9808) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 27606) [M::mem_pestat] mean and std.dev: (5921.90, 4392.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 36505) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 325, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (305.74, 106.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686188 reads in 186.468 CPU sec, 5.908 real sec sorting block 105, #reads = 1074347 mem = 536877439 peak = 1092800512 [M::mem_process_seqs] Processed 687000 reads in 124.820 CPU sec, 3.957 real sec sorting block 106, #reads = 1074576 mem = 536878072 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 283593, 11, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 197, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (248.83, 130.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 300, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (320.68, 156.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1371, 1371, 1750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (613, 2508) [M::mem_pestat] mean and std.dev: (1536.50, 473.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3431) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 335, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (306.83, 123.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1086) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684676 sequences (100000071 bp) in 3.144 CPU sec, 3.233 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 283413, 9, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 280, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (280.92, 134.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 307, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (326.33, 157.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (269, 381, 584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1214) [M::mem_pestat] mean and std.dev: (345.74, 145.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1529) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686524 sequences (100000090 bp) in 2.460 CPU sec, 2.452 real sec... [M::mem_process_seqs] Processed 686242 reads in 188.433 CPU sec, 5.945 real sec [M::mem_process_seqs] Processed 685052 reads in 123.293 CPU sec, 3.894 real sec sorting block 107, #reads = 1075158 mem = 536878514 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 283033, 8, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 289, 834) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2036) [M::mem_pestat] mean and std.dev: (524.27, 416.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 311, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923) [M::mem_pestat] mean and std.dev: (330.63, 160.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1164) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 240, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (259.64, 126.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683890 sequences (100000125 bp) in 3.057 CPU sec, 3.163 real sec... [M::process] read 684140 sequences (100000237 bp) in 1.930 CPU sec, 1.928 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 284391, 9, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 230, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1139) [M::mem_pestat] mean and std.dev: (205.27, 107.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 297, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (321.53, 160.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (254, 494, 1038) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2606) [M::mem_pestat] mean and std.dev: (661.97, 510.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3390) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684676 reads in 185.839 CPU sec, 5.879 real sec sorting block 108, #reads = 1075412 mem = 536877869 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282773, 6, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 224, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (219.89, 56.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 317, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (335.95, 163.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1187) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 336, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062) [M::mem_pestat] mean and std.dev: (332.10, 147.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1342) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683908 sequences (100000117 bp) in 2.641 CPU sec, 2.650 real sec... [M::mem_process_seqs] Processed 683890 reads in 109.833 CPU sec, 3.738 real sec [M::mem_process_seqs] Processed 686524 reads in 261.022 CPU sec, 8.549 real sec sorting block 109, #reads = 1076322 mem = 536882659 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282877, 2, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 242, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (251.82, 85.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 317, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950) [M::mem_pestat] mean and std.dev: (337.68, 165.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1199) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (290, 358, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055) [M::mem_pestat] mean and std.dev: (378.23, 180.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1310) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684174 sequences (100000107 bp) in 3.052 CPU sec, 3.165 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 283033, 10, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 286, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (286.21, 122.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 317, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959) [M::mem_pestat] mean and std.dev: (339.31, 167.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1211) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (82, 252, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1183) [M::mem_pestat] mean and std.dev: (289.60, 225.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1550) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 286, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (273.63, 119.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684140 reads in 209.421 CPU sec, 6.633 real sec [M::process] read 683682 sequences (100000014 bp) in 2.119 CPU sec, 2.157 real sec... [M::mem_process_seqs] Processed 683908 reads in 128.181 CPU sec, 4.036 real sec sorting block 110, #reads = 1075870 mem = 536881163 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 283478, 6, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 202, 599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1447) [M::mem_pestat] mean and std.dev: (317.08, 214.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1871) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 317, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950) [M::mem_pestat] mean and std.dev: (338.22, 166.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1199) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 337, 667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1543) [M::mem_pestat] mean and std.dev: (425.25, 329.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1981) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682868 sequences (100000146 bp) in 3.465 CPU sec, 3.663 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 282566, 14, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 258, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (278.18, 150.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 319, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951) [M::mem_pestat] mean and std.dev: (339.52, 166.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1200) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (197, 501, 1952) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5462) [M::mem_pestat] mean and std.dev: (824.64, 754.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7217) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 324, 512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1134) [M::mem_pestat] mean and std.dev: (368.90, 215.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1445) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684174 reads in 181.914 CPU sec, 5.764 real sec [M::mem_process_seqs] Processed 683682 reads in 123.905 CPU sec, 3.900 real sec [M::process] read 683104 sequences (100000075 bp) in 2.698 CPU sec, 2.739 real sec... sorting block 111, #reads = 1075481 mem = 536880493 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 282548, 4, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 329, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (283.11, 115.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 321, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953) [M::mem_pestat] mean and std.dev: (341.48, 166.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1202) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 261, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (276.31, 119.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1096) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683374 sequences (100000275 bp) in 2.914 CPU sec, 3.031 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 282351, 20, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 290, 652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1530) [M::mem_pestat] mean and std.dev: (393.30, 356.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1969) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 325, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972) [M::mem_pestat] mean and std.dev: (345.27, 169.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1227) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (430, 794, 1123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2509) [M::mem_pestat] mean and std.dev: (814.10, 451.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3202) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (293.60, 141.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682868 reads in 185.251 CPU sec, 5.872 real sec sorting block 112, #reads = 1074690 mem = 536882526 peak = 1092800512 [M::process] read 687780 sequences (100000225 bp) in 2.665 CPU sec, 2.694 real sec... [M::mem_process_seqs] Processed 683104 reads in 126.259 CPU sec, 3.966 real sec sorting block 113, #reads = 1073124 mem = 536876932 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 283204, 2, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 236, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (236.96, 95.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 989) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 321, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956) [M::mem_pestat] mean and std.dev: (341.39, 167.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1206) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 319, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (292.66, 161.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689904 sequences (100000218 bp) in 3.161 CPU sec, 3.257 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 284084, 6, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 241, 573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1381) [M::mem_pestat] mean and std.dev: (294.33, 178.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1785) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 295, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (318.71, 159.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (276, 374, 535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053) [M::mem_pestat] mean and std.dev: (431.17, 268.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1503) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688370 sequences (100000126 bp) in 2.350 CPU sec, 2.351 real sec... [M::mem_process_seqs] Processed 683374 reads in 194.156 CPU sec, 6.142 real sec [M::mem_process_seqs] Processed 687780 reads in 137.564 CPU sec, 4.339 real sec sorting block 114, #reads = 1070786 mem = 536878012 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 285210, 10, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 355, 694) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1732) [M::mem_pestat] mean and std.dev: (361.74, 284.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2251) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 283, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (308.29, 155.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1091) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (518, 518, 1316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2912) [M::mem_pestat] mean and std.dev: (750.33, 526.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3710) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 203, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962) [M::mem_pestat] mean and std.dev: (239.67, 150.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1236) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688042 sequences (100000162 bp) in 2.992 CPU sec, 3.095 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 284945, 1, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 344, 552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1208) [M::mem_pestat] mean and std.dev: (402.20, 235.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 291, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (313.90, 156.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 424, 764) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1834) [M::mem_pestat] mean and std.dev: (465.69, 402.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2369) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687278 sequences (100000267 bp) in 2.410 CPU sec, 2.405 real sec... [M::mem_process_seqs] Processed 689904 reads in 185.364 CPU sec, 5.848 real sec sorting block 115, #reads = 1073933 mem = 536879638 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 285486, 8, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 238, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023) [M::mem_pestat] mean and std.dev: (272.61, 131.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1309) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 294, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (315.21, 155.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 246, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (257.70, 128.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686256 sequences (100000295 bp) in 2.613 CPU sec, 2.619 real sec... [M::mem_process_seqs] Processed 688042 reads in 102.521 CPU sec, 3.250 real sec [M::mem_process_seqs] Processed 688370 reads in 254.333 CPU sec, 8.061 real sec sorting block 116, #reads = 1080360 mem = 536880479 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 284434, 4, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 241, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (284.37, 134.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 297, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (317.86, 156.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 226, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (242.67, 102.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686082 sequences (100000093 bp) in 2.893 CPU sec, 2.989 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 284283, 12, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 299, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (267.08, 118.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 302, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (321.91, 157.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (108, 248, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1353) [M::mem_pestat] mean and std.dev: (238.90, 192.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1768) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 386, 561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1201) [M::mem_pestat] mean and std.dev: (411.79, 223.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1521) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687278 reads in 192.590 CPU sec, 6.099 real sec [M::mem_process_seqs] Processed 686256 reads in 115.344 CPU sec, 3.639 real sec sorting block 117, #reads = 1080016 mem = 536881766 peak = 1092800512 [M::process] read 686236 sequences (100000168 bp) in 2.802 CPU sec, 2.887 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 283937, 1, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 315, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (260.95, 111.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 304, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (324.53, 159.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1143) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 239, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (224.43, 77.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685744 sequences (100000278 bp) in 2.660 CPU sec, 2.757 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283938, 1, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 257, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (238.65, 81.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 305, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (326.44, 161.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1159) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 377, 650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1568) [M::mem_pestat] mean and std.dev: (405.04, 240.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2027) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686082 reads in 169.354 CPU sec, 5.368 real sec [M::mem_process_seqs] Processed 686236 reads in 114.838 CPU sec, 3.611 real sec sorting block 118, #reads = 1079271 mem = 536880987 peak = 1092800512 [M::process] read 685394 sequences (100000115 bp) in 2.590 CPU sec, 2.632 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 282860, 6, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 195, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (187.24, 73.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 307, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (328.03, 162.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 318, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1166) [M::mem_pestat] mean and std.dev: (338.62, 188.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1501) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685366 sequences (100000127 bp) in 2.979 CPU sec, 3.082 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 283505, 14, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 238, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (233.55, 101.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 309, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (329.47, 161.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1158) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (395, 645, 645) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1145) [M::mem_pestat] mean and std.dev: (491.58, 238.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1446) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 340, 577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1363) [M::mem_pestat] mean and std.dev: (361.08, 288.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1756) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685744 reads in 171.614 CPU sec, 5.436 real sec sorting block 119, #reads = 1079999 mem = 536878545 peak = 1092800512 [M::mem_process_seqs] Processed 685394 reads in 116.402 CPU sec, 3.660 real sec [M::process] read 685160 sequences (100000248 bp) in 2.669 CPU sec, 2.714 real sec... sorting block 120, #reads = 1078078 mem = 536879206 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 283644, 9, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 326, 590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1424) [M::mem_pestat] mean and std.dev: (357.38, 212.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1841) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 308, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (328.08, 160.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 256, 573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1333) [M::mem_pestat] mean and std.dev: (305.26, 211.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1713) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685486 sequences (100000257 bp) in 3.053 CPU sec, 3.204 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 284034, 5, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 310, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1109) [M::mem_pestat] mean and std.dev: (332.71, 179.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 309, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (328.55, 160.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1151) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 271, 585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1393) [M::mem_pestat] mean and std.dev: (332.57, 217.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1797) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685366 reads in 177.849 CPU sec, 5.640 real sec [M::mem_process_seqs] Processed 685160 reads in 123.403 CPU sec, 3.881 real sec [M::process] read 685096 sequences (100000022 bp) in 2.668 CPU sec, 2.717 real sec... sorting block 121, #reads = 1079154 mem = 536879889 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283914, 8, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 222, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041) [M::mem_pestat] mean and std.dev: (310.92, 217.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1333) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 306, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (325.88, 159.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 314, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1095) [M::mem_pestat] mean and std.dev: (333.03, 158.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1380) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684522 sequences (100000074 bp) in 3.132 CPU sec, 3.275 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 283195, 4, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 251, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1065) [M::mem_pestat] mean and std.dev: (310.46, 222.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1367) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 311, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (330.77, 162.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1167) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 265, 1133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2963) [M::mem_pestat] mean and std.dev: (781.37, 931.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4506) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685486 reads in 175.893 CPU sec, 5.570 real sec [M::mem_process_seqs] Processed 685096 reads in 118.987 CPU sec, 3.739 real sec [M::process] read 684048 sequences (100000122 bp) in 2.604 CPU sec, 2.650 real sec... sorting block 122, #reads = 1076495 mem = 536879588 peak = 1092800512 [M::process] read 684352 sequences (100000144 bp) in 2.834 CPU sec, 2.927 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 283662, 6, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 219, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (225.45, 103.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 316, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931) [M::mem_pestat] mean and std.dev: (334.34, 162.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 280, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004) [M::mem_pestat] mean and std.dev: (323.38, 165.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1270) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684048 reads in 97.915 CPU sec, 3.067 real sec sorting block 123, #reads = 1077389 mem = 536880644 peak = 1092800512 [M::process] read 683986 sequences (100000062 bp) in 2.583 CPU sec, 2.629 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 283487, 1, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 206, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (225.00, 106.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 312, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (330.76, 160.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1150) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (238, 344, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (311.16, 122.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 282837, 2, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 269, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (298.28, 142.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 314, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (333.50, 162.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1170) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 264, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (290.30, 159.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684522 reads in 269.702 CPU sec, 8.527 real sec [M::process] read 683670 sequences (100000280 bp) in 2.124 CPU sec, 2.153 real sec... [M::mem_process_seqs] Processed 684352 reads in 139.125 CPU sec, 4.382 real sec sorting block 124, #reads = 1077117 mem = 536877621 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 282685, 19, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 224, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (226.39, 108.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 317, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (337.32, 165.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1184) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1931, 2351, 3625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7013) [M::mem_pestat] mean and std.dev: (2384.89, 1320.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8707) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 204, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977) [M::mem_pestat] mean and std.dev: (250.21, 125.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1243) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683636 sequences (100000226 bp) in 3.024 CPU sec, 3.127 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 282714, 8, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 522, 1215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3279) [M::mem_pestat] mean and std.dev: (655.15, 632.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4311) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 317, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (337.53, 164.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1184) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 293, 662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1558) [M::mem_pestat] mean and std.dev: (372.91, 229.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2006) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683986 reads in 169.351 CPU sec, 5.368 real sec [M::mem_process_seqs] Processed 683670 reads in 114.646 CPU sec, 3.603 real sec sorting block 125, #reads = 1076784 mem = 536879126 peak = 1092800512 [M::process] read 684040 sequences (100000196 bp) in 2.479 CPU sec, 2.540 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 282405, 11, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 242, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965) [M::mem_pestat] mean and std.dev: (318.68, 219.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1230) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 317, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (335.48, 162.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (848, 2494, 2554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5966) [M::mem_pestat] mean and std.dev: (1858.82, 1005.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7672) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (251, 374, 583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1247) [M::mem_pestat] mean and std.dev: (394.54, 205.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1579) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 126, #reads = 1076297 mem = 536877007 peak = 1092800512 [M::process] read 683238 sequences (100000241 bp) in 2.441 CPU sec, 2.548 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 282965, 3, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 357, 596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1346) [M::mem_pestat] mean and std.dev: (415.98, 266.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 315, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (336.07, 164.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1183) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (274, 330, 922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2218) [M::mem_pestat] mean and std.dev: (691.34, 693.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3467) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683526 sequences (100000242 bp) in 1.790 CPU sec, 1.791 real sec... [M::mem_process_seqs] Processed 683636 reads in 172.229 CPU sec, 5.466 real sec [M::mem_process_seqs] Processed 684040 reads in 117.205 CPU sec, 3.707 real sec sorting block 127, #reads = 1076748 mem = 536877947 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 283285, 4, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 318, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957) [M::mem_pestat] mean and std.dev: (321.96, 153.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1214) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 317, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936) [M::mem_pestat] mean and std.dev: (336.79, 163.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1180) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 302, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1228) [M::mem_pestat] mean and std.dev: (288.69, 133.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1576) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683536 sequences (100000208 bp) in 3.226 CPU sec, 3.322 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 282725, 9, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 298, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995) [M::mem_pestat] mean and std.dev: (292.91, 185.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1274) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 316, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (336.69, 164.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1187) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (271, 349, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979) [M::mem_pestat] mean and std.dev: (372.16, 162.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1215) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683238 reads in 174.234 CPU sec, 5.490 real sec [M::process] read 683716 sequences (100000067 bp) in 2.531 CPU sec, 2.542 real sec... [M::mem_process_seqs] Processed 683526 reads in 118.737 CPU sec, 3.735 real sec sorting block 128, #reads = 1076773 mem = 536882431 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282712, 6, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 325, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (302.94, 113.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 315, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (335.91, 163.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1183) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 279, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1081) [M::mem_pestat] mean and std.dev: (326.57, 183.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1373) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683556 sequences (100000099 bp) in 2.818 CPU sec, 2.886 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 282519, 13, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 331, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1142) [M::mem_pestat] mean and std.dev: (305.46, 164.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 316, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (336.72, 165.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1187) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (231, 444, 6562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 19224) [M::mem_pestat] mean and std.dev: (2120.77, 2692.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 25555) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 275, 965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2561) [M::mem_pestat] mean and std.dev: (381.61, 333.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3359) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683536 reads in 172.369 CPU sec, 5.441 real sec [M::process] read 684348 sequences (100000079 bp) in 2.431 CPU sec, 2.448 real sec... [M::mem_process_seqs] Processed 683716 reads in 117.089 CPU sec, 3.675 real sec sorting block 129, #reads = 1074660 mem = 536879794 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 282899, 10, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 302, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029) [M::mem_pestat] mean and std.dev: (268.71, 185.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1322) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 317, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948) [M::mem_pestat] mean and std.dev: (338.59, 166.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1196) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (383, 1058, 1058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2408) [M::mem_pestat] mean and std.dev: (967.10, 639.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3524) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (281, 316, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (284.91, 106.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683782 sequences (100000019 bp) in 3.215 CPU sec, 3.316 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 282842, 5, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 271, 600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1474) [M::mem_pestat] mean and std.dev: (343.23, 262.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1911) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 314, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937) [M::mem_pestat] mean and std.dev: (335.29, 165.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1182) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 370, 611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1361) [M::mem_pestat] mean and std.dev: (376.78, 220.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1736) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683556 reads in 174.081 CPU sec, 5.502 real sec sorting block 130, #reads = 1075072 mem = 536887756 peak = 1092800512 [M::mem_process_seqs] Processed 684348 reads in 119.470 CPU sec, 3.746 real sec [M::process] read 683270 sequences (100000097 bp) in 2.604 CPU sec, 2.628 real sec... sorting block 131, #reads = 1074045 mem = 536876339 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 283184, 15, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 228, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987) [M::mem_pestat] mean and std.dev: (263.44, 169.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1259) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 314, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932) [M::mem_pestat] mean and std.dev: (335.21, 164.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1175) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (85, 285, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1306) [M::mem_pestat] mean and std.dev: (190.42, 166.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1713) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 287, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (302.02, 135.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688840 sequences (100000090 bp) in 2.937 CPU sec, 3.059 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 282802, 14, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 394, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004) [M::mem_pestat] mean and std.dev: (349.89, 178.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1271) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 320, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950) [M::mem_pestat] mean and std.dev: (341.05, 168.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1198) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (544, 700, 1814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4354) [M::mem_pestat] mean and std.dev: (1076.79, 775.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5624) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 301, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1109) [M::mem_pestat] mean and std.dev: (348.63, 231.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1422) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683782 reads in 187.940 CPU sec, 5.950 real sec [M::process] read 693836 sequences (100000074 bp) in 2.623 CPU sec, 2.648 real sec... [M::mem_process_seqs] Processed 683270 reads in 131.328 CPU sec, 4.130 real sec sorting block 132, #reads = 1073076 mem = 536878937 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 284773, 4, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 374, 567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1367) [M::mem_pestat] mean and std.dev: (344.24, 218.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1767) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 287, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (313.20, 158.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 298, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804) [M::mem_pestat] mean and std.dev: (299.61, 122.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 691862 sequences (100000077 bp) in 2.990 CPU sec, 3.087 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 286533, 13, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 210, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (206.62, 72.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 264, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (290.11, 146.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (306, 541, 4538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13002) [M::mem_pestat] mean and std.dev: (1782.15, 2502.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17234) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 219, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (230.93, 79.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 690408 sequences (100000195 bp) in 2.440 CPU sec, 2.435 real sec... [M::mem_process_seqs] Processed 688840 reads in 206.258 CPU sec, 6.527 real sec [M::mem_process_seqs] Processed 693836 reads in 137.068 CPU sec, 4.327 real sec sorting block 133, #reads = 1065805 mem = 536879619 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 286339, 5, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 286, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1155) [M::mem_pestat] mean and std.dev: (274.21, 169.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 272, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837) [M::mem_pestat] mean and std.dev: (297.18, 148.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1057) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 452, 939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2399) [M::mem_pestat] mean and std.dev: (653.89, 556.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3129) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689004 sequences (100000177 bp) in 2.767 CPU sec, 2.974 real sec... [M::process] read 688616 sequences (100000265 bp) in 1.900 CPU sec, 1.902 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 285859, 7, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 269, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (295.71, 130.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 281, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (303.28, 149.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1074) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 286, 593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1363) [M::mem_pestat] mean and std.dev: (332.86, 175.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1748) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691862 reads in 189.868 CPU sec, 6.026 real sec sorting block 134, #reads = 1074718 mem = 536879883 peak = 1092800512 [M::mem_process_seqs] Processed 690408 reads in 126.028 CPU sec, 3.994 real sec sorting block 135, #reads = 1076195 mem = 536878031 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 285330, 9, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 223, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (244.38, 104.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 287, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849) [M::mem_pestat] mean and std.dev: (306.79, 148.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1070) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 242, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (279.03, 133.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688236 sequences (100000178 bp) in 3.253 CPU sec, 3.333 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 285252, 3, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 240, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (229.08, 90.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 997) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 290, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (309.51, 150.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1087) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 288, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (274.05, 89.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689004 reads in 186.493 CPU sec, 5.874 real sec [M::process] read 688058 sequences (100000123 bp) in 2.460 CPU sec, 2.462 real sec... [M::mem_process_seqs] Processed 688616 reads in 126.650 CPU sec, 3.984 real sec sorting block 136, #reads = 1075308 mem = 536878547 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 284617, 8, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 195, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (242.25, 194.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 292, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (313.46, 154.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 264, 544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1290) [M::mem_pestat] mean and std.dev: (286.44, 143.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1663) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687058 sequences (100000119 bp) in 2.899 CPU sec, 2.982 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 284729, 8, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 237, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1186) [M::mem_pestat] mean and std.dev: (349.92, 233.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 294, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (315.94, 155.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 279, 617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1477) [M::mem_pestat] mean and std.dev: (378.24, 245.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1907) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686412 sequences (100000291 bp) in 2.499 CPU sec, 2.493 real sec... [M::mem_process_seqs] Processed 688236 reads in 197.399 CPU sec, 6.240 real sec [M::mem_process_seqs] Processed 688058 reads in 123.327 CPU sec, 3.885 real sec sorting block 137, #reads = 1073123 mem = 536882345 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 284019, 6, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 218, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018) [M::mem_pestat] mean and std.dev: (328.31, 249.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1325) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 298, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (318.93, 155.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 291, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1079) [M::mem_pestat] mean and std.dev: (291.84, 146.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1376) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686332 sequences (100000282 bp) in 2.693 CPU sec, 2.775 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 284235, 6, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (325, 466, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (402.23, 149.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 302, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (322.76, 158.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 259, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (235.71, 81.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686646 sequences (100000124 bp) in 1.890 CPU sec, 1.882 real sec... [M::mem_process_seqs] Processed 687058 reads in 154.117 CPU sec, 4.864 real sec sorting block 138, #reads = 1074849 mem = 536879901 peak = 1092800512 [M::mem_process_seqs] Processed 686412 reads in 101.514 CPU sec, 3.199 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 284094, 9, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 262, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (251.82, 130.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 301, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (321.79, 157.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1138) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 300, 676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1634) [M::mem_pestat] mean and std.dev: (309.87, 220.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2113) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685186 sequences (100000245 bp) in 2.597 CPU sec, 2.686 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 284207, 3, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 258, 582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1428) [M::mem_pestat] mean and std.dev: (311.00, 232.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1851) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 297, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (316.94, 152.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 250, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (244.04, 107.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683770 sequences (100000206 bp) in 1.920 CPU sec, 1.917 real sec... [M::mem_process_seqs] Processed 686332 reads in 166.639 CPU sec, 5.273 real sec sorting block 139, #reads = 1074280 mem = 536880410 peak = 1092800512 [M::mem_process_seqs] Processed 686646 reads in 114.876 CPU sec, 3.628 real sec sorting block 140, #reads = 1073625 mem = 536878530 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 283265, 5, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 280, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1143) [M::mem_pestat] mean and std.dev: (298.31, 196.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 306, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (325.47, 157.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1142) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 276, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (57, 477) [M::mem_pestat] mean and std.dev: (261.37, 97.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685158 sequences (100000176 bp) in 2.896 CPU sec, 2.956 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283164, 9, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 326, 1333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3559) [M::mem_pestat] mean and std.dev: (757.25, 829.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 315, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (332.03, 157.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (253, 392, 1050) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2644) [M::mem_pestat] mean and std.dev: (528.10, 406.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3441) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685186 reads in 167.815 CPU sec, 5.307 real sec [M::mem_process_seqs] Processed 683770 reads in 113.617 CPU sec, 3.563 real sec [M::process] read 685224 sequences (100000249 bp) in 2.590 CPU sec, 2.609 real sec... sorting block 141, #reads = 1072908 mem = 536880116 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 283911, 6, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 294, 829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2117) [M::mem_pestat] mean and std.dev: (589.13, 616.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3053) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 308, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (326.31, 157.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (254, 287, 680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1532) [M::mem_pestat] mean and std.dev: (388.41, 276.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1958) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684848 sequences (100000079 bp) in 2.833 CPU sec, 2.920 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283100, 9, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 263, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (306.96, 159.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1096) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 304, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (323.59, 155.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 250, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (257.21, 155.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685158 reads in 168.322 CPU sec, 5.328 real sec [M::mem_process_seqs] Processed 685224 reads in 114.044 CPU sec, 3.575 real sec [M::process] read 684488 sequences (100000301 bp) in 2.633 CPU sec, 2.657 real sec... sorting block 142, #reads = 1074617 mem = 536881925 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 283046, 3, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 299, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1148) [M::mem_pestat] mean and std.dev: (361.69, 265.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 307, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (326.30, 157.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 380, 748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1784) [M::mem_pestat] mean and std.dev: (454.06, 351.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2302) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684370 sequences (100000077 bp) in 3.038 CPU sec, 3.119 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 283346, 9, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 218, 593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1495) [M::mem_pestat] mean and std.dev: (272.05, 194.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1946) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 309, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (327.57, 156.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 379, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (302.79, 126.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1143) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684848 reads in 167.813 CPU sec, 5.315 real sec [M::mem_process_seqs] Processed 684488 reads in 112.728 CPU sec, 3.537 real sec sorting block 143, #reads = 1073157 mem = 536879268 peak = 1092800512 [M::process] read 684508 sequences (100000137 bp) in 2.655 CPU sec, 2.706 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282561, 6, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 291, 804) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2128) [M::mem_pestat] mean and std.dev: (419.32, 355.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2790) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 310, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (329.05, 158.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 428, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (343.85, 160.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1188) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 144, #reads = 1074924 mem = 536882627 peak = 1092800512 [M::process] read 684360 sequences (100000104 bp) in 2.695 CPU sec, 2.788 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 283239, 9, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 394, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066) [M::mem_pestat] mean and std.dev: (365.20, 194.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1356) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 309, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (328.70, 158.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 239, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (235.56, 83.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684370 reads in 166.926 CPU sec, 5.291 real sec [M::mem_process_seqs] Processed 684508 reads in 112.856 CPU sec, 3.547 real sec [M::process] read 684346 sequences (100000073 bp) in 2.636 CPU sec, 2.681 real sec... sorting block 145, #reads = 1075238 mem = 536881843 peak = 1092800512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 283013, 5, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 335, 596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1336) [M::mem_pestat] mean and std.dev: (451.69, 316.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 310, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921) [M::mem_pestat] mean and std.dev: (330.88, 160.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 231, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (250.36, 90.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684104 sequences (100000276 bp) in 2.897 CPU sec, 2.971 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 282929, 7, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 186, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (229.15, 112.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 311, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (332.32, 162.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1173) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (135, 248, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (234.19, 105.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684360 reads in 170.829 CPU sec, 5.409 real sec [M::process] read 684348 sequences (100000129 bp) in 2.510 CPU sec, 2.554 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282689, 5, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 334, 616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1476) [M::mem_pestat] mean and std.dev: (368.70, 248.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1906) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 312, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (332.85, 162.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1173) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 254, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1130) [M::mem_pestat] mean and std.dev: (330.12, 212.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1444) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683194 sequences (100000182 bp) in 1.708 CPU sec, 1.718 real sec... [M::mem_process_seqs] Processed 684104 reads in 97.383 CPU sec, 3.091 real sec sorting block 146, #reads = 1075353 mem = 536879487 peak = 1092800512 [M::mem_process_seqs] Processed 684346 reads in 233.571 CPU sec, 7.386 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 282753, 5, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 299, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846) [M::mem_pestat] mean and std.dev: (298.35, 148.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 311, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (332.53, 162.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1173) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 313, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (299.54, 140.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 147, #reads = 1075933 mem = 536880130 peak = 1129287680 [M::process] read 682390 sequences (100000013 bp) in 2.980 CPU sec, 3.083 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 282346, 5, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 208, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848) [M::mem_pestat] mean and std.dev: (283.47, 172.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1071) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 317, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (338.85, 166.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1193) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 247, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (265.90, 125.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684348 reads in 189.473 CPU sec, 6.002 real sec [M::mem_process_seqs] Processed 683194 reads in 117.959 CPU sec, 3.718 real sec [M::process] read 683260 sequences (100000058 bp) in 2.328 CPU sec, 2.372 real sec... sorting block 148, #reads = 1074697 mem = 536882683 peak = 1129287680 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282511, 10, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 315, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (282.07, 149.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 324, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953) [M::mem_pestat] mean and std.dev: (343.82, 166.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1201) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (145, 1729, 1801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5113) [M::mem_pestat] mean and std.dev: (1344.60, 1357.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6775) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 271, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (341.92, 166.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 404976 sequences (59193914 bp) in 2.154 CPU sec, 2.241 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 282260, 10, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 483, 583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1455) [M::mem_pestat] mean and std.dev: (361.87, 193.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1891) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 318, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940) [M::mem_pestat] mean and std.dev: (338.24, 165.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1185) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1041, 3330, 3330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7908) [M::mem_pestat] mean and std.dev: (2704.80, 2110.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11146) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 323, 1073) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2801) [M::mem_pestat] mean and std.dev: (601.44, 659.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3665) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682390 reads in 167.671 CPU sec, 5.391 real sec [M::mem_process_seqs] Processed 683260 reads in 113.497 CPU sec, 3.667 real sec sorting block 149, #reads = 1075247 mem = 536880156 peak = 1129287680 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 167323, 11, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 236, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (255.65, 129.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1143) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 313, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (333.39, 162.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1170) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (812, 909, 2552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6032) [M::mem_pestat] mean and std.dev: (1192.73, 889.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7772) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 407, 1032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2644) [M::mem_pestat] mean and std.dev: (533.34, 394.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3450) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 404976 reads in 61.220 CPU sec, 2.025 real sec sorting block 150, #reads = 1074491 mem = 536885454 peak = 1129287680 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: 1f8bfbff fffab20b extended: f1bf07ab 1a407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8559C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 161967094 overall: 69923983360 mem 25714.591 user 647.917 sys 888.417 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_7OW-T1-TDNA-1_A23MHWWLT4_1.L007\tPU:L007\tSM:1136_7OW-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_7OW-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1136_7OW-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 1136_7OW-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 888.446 sec; CPU: 26362.512 sec sorting block 151, #reads = 161928 mem = 81288849 peak = 1129287680 uniq header count 1 merging 152 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: 1f8bfbff fffab20b extended: f1bf07ab 1a407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8559C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 9036122592 output reads: 162873098 bam_mem_sort: 152 calls 91.375 user 1.920 sys 94.184 real bam_write: 152 calls 49.689 user 3.401 sys 70.280 real execute: 1 calls 37.619 user 1.324 sys 952.333 real merge_files: 1 calls 37.591 user 1.293 sys 63.801 real parse_chunk: 2419 calls 139.759 user 32.459 sys 181.559 real read_chunk: 6526 calls 70.868 user 45.284 sys 821.729 real sort_block: 1 calls 50.569 user 44.480 sys 793.358 real write_chunk: 4105 calls 37.235 user 0.943 sys 62.760 real overall: 3321741312 mem 2548.904 user 101.099 sys 952.352 real ls: cannot access '*.cram': No such file or directory