cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1029_N5D-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1691.946 CPU sec, 360.733 real sec
[M::process] read 683892 sequences (100000187 bp) in 0.962 CPU sec, 0.962 real sec...
[M::process] read 682358 sequences (100000064 bp) in 1.160 CPU sec, 1.162 real sec...
[M::process] read 681508 sequences (100000229 bp) in 1.178 CPU sec, 1.182 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278561, 6, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 240, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (258.56, 121.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 885)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 334, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (350.47, 169.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 291, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (270.86, 98.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681046 sequences (100000031 bp) in 1.129 CPU sec, 1.129 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 278530, 2, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 285, 1507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4173)
[M::mem_pestat] mean and std.dev: (638.37, 736.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5506)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 346, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (360.04, 172.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 209, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (272.74, 174.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1087)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 277699, 11, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 277, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (260.41, 125.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1323)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 350, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (362.46, 171.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (213, 213, 695)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1659)
[M::mem_pestat] mean and std.dev: (284.11, 233.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2141)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 286, 809)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2055)
[M::mem_pestat] mean and std.dev: (478.65, 492.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2678)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683892 reads in 231.765 CPU sec, 7.407 real sec
[M::mem_process_seqs] Processed 682358 reads in 157.949 CPU sec, 5.103 real sec
[M::mem_process_seqs] Processed 681508 reads in 173.235 CPU sec, 5.611 real sec
sorting block 0, #reads = 1070200 mem = 536879128 peak = 600322048
[M::process] read 681962 sequences (100000202 bp) in 1.690 CPU sec, 1.809 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 277893, 6, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 189, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (231.46, 114.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 358, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (369.53, 174.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 242, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561)
[M::mem_pestat] mean and std.dev: (267.86, 82.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 676)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681738 sequences (100000084 bp) in 1.260 CPU sec, 1.286 real sec...
[M::process] read 681490 sequences (100000141 bp) in 1.090 CPU sec, 1.090 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 277871, 5, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 257, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690)
[M::mem_pestat] mean and std.dev: (261.59, 111.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 349, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (363.06, 174.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 309, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (282.55, 130.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 877)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681046 reads in 148.072 CPU sec, 4.709 real sec
sorting block 1, #reads = 1071514 mem = 536881163 peak = 812658688
[M::mem_process_seqs] Processed 681962 reads in 95.814 CPU sec, 3.049 real sec
sorting block 2, #reads = 1071081 mem = 536879476 peak = 880779264
[M::process] read 681806 sequences (100000007 bp) in 1.573 CPU sec, 1.655 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 277347, 6, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 236, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (261.93, 131.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 350, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (364.06, 173.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 285, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (288.24, 157.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681888 sequences (100000093 bp) in 1.214 CPU sec, 1.225 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 278110, 9, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 240, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1223)
[M::mem_pestat] mean and std.dev: (338.93, 271.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1574)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 339, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (352.67, 165.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 525, 543)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1215)
[M::mem_pestat] mean and std.dev: (443.92, 261.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1551)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278060, 7, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (196, 408, 543)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1237)
[M::mem_pestat] mean and std.dev: (406.79, 240.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1584)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 347, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (361.65, 171.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 349, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (315.73, 111.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 981)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681738 reads in 215.640 CPU sec, 6.896 real sec
[M::mem_process_seqs] Processed 681806 reads in 161.767 CPU sec, 5.201 real sec
[M::mem_process_seqs] Processed 681490 reads in 176.659 CPU sec, 5.691 real sec
sorting block 3, #reads = 1069227 mem = 536880669 peak = 880779264
[M::process] read 681102 sequences (100000100 bp) in 1.972 CPU sec, 2.121 real sec...
sorting block 4, #reads = 1069619 mem = 536879000 peak = 880779264
[M::process] read 680818 sequences (100000192 bp) in 1.326 CPU sec, 1.351 real sec...
[M::process] read 680536 sequences (100000185 bp) in 1.180 CPU sec, 1.177 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 276954, 1, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 284, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (272.29, 147.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1280)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 340, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (355.97, 167.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 301, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (280.77, 101.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681102 reads in 84.173 CPU sec, 2.654 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 277871, 7, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 245, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (282.89, 164.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1280)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 337, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (352.05, 166.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 273, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (283.38, 124.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682076 sequences (100000217 bp) in 1.476 CPU sec, 1.484 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 277854, 7, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (284, 396, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (354.95, 141.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 984)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 343, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (357.43, 168.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 267, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (25, 520)
[M::mem_pestat] mean and std.dev: (263.17, 73.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 619)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681888 reads in 236.303 CPU sec, 7.525 real sec
sorting block 5, #reads = 1068780 mem = 536879652 peak = 880779264
[M::mem_process_seqs] Processed 680818 reads in 112.032 CPU sec, 3.588 real sec
sorting block 6, #reads = 1069375 mem = 536878044 peak = 880779264
[M::process] read 682142 sequences (100000184 bp) in 1.692 CPU sec, 1.790 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 277578, 2, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 271, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564)
[M::mem_pestat] mean and std.dev: (241.36, 84.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 688)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 343, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (358.88, 168.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 318, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (277.68, 106.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 967)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681348 sequences (100000025 bp) in 1.240 CPU sec, 1.243 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 278171, 2, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 300, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (301.14, 173.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 346, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (360.75, 173.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 241, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (232.44, 95.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 277968, 5, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 250, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (235.81, 98.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 341, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (356.00, 169.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 312, 717)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1701)
[M::mem_pestat] mean and std.dev: (425.46, 325.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2193)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680536 reads in 207.826 CPU sec, 6.641 real sec
[M::mem_process_seqs] Processed 682142 reads in 155.376 CPU sec, 4.986 real sec
[M::mem_process_seqs] Processed 682076 reads in 170.605 CPU sec, 5.494 real sec
sorting block 7, #reads = 1070143 mem = 536881614 peak = 880779264
[M::process] read 680754 sequences (100000265 bp) in 1.796 CPU sec, 1.928 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 278019, 2, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 307, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1071)
[M::mem_pestat] mean and std.dev: (333.11, 188.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1370)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 354, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (368.18, 176.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 310, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (307.69, 100.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681012 sequences (100000175 bp) in 1.545 CPU sec, 1.685 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 278279, 12, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (219, 279, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (291.68, 117.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 360, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (372.65, 176.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (439, 1310, 2392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6298)
[M::mem_pestat] mean and std.dev: (1269.33, 1039.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8251)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 277, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (286.51, 127.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 681336 sequences (100000023 bp) in 1.240 CPU sec, 1.238 real sec...
[M::mem_process_seqs] Processed 681348 reads in 146.772 CPU sec, 4.672 real sec
sorting block 8, #reads = 1070562 mem = 536880392 peak = 880779264
[M::mem_process_seqs] Processed 680754 reads in 95.046 CPU sec, 3.015 real sec
sorting block 9, #reads = 1071143 mem = 536876003 peak = 915648512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 277543, 12, 57)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 287, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (259.13, 137.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1313)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 357, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (369.75, 175.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (648, 648, 1383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2853)
[M::mem_pestat] mean and std.dev: (745.27, 423.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3588)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 350, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1102)
[M::mem_pestat] mean and std.dev: (332.10, 165.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1399)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 681330 sequences (100000277 bp) in 1.807 CPU sec, 1.942 real sec...
[M::process] read 681476 sequences (100000126 bp) in 1.300 CPU sec, 1.296 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 278210, 5, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (216, 343, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (360.92, 210.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 351, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (365.47, 174.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 332, 551)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1275)
[M::mem_pestat] mean and std.dev: (344.26, 172.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1637)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681012 reads in 147.947 CPU sec, 4.716 real sec
[M::mem_process_seqs] Processed 681336 reads in 94.664 CPU sec, 3.016 real sec
sorting block 10, #reads = 1071037 mem = 536878730 peak = 915648512
[M::process] read 682514 sequences (100000294 bp) in 1.693 CPU sec, 1.785 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 277234, 2, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 259, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (247.92, 96.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 348, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (363.17, 173.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 255, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (259.16, 109.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681520 sequences (100000203 bp) in 1.290 CPU sec, 1.292 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 277928, 6, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 187, 235)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407)
[M::mem_pestat] mean and std.dev: (188.69, 69.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 493)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 343, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (359.04, 171.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 247, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (284.40, 126.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 278102, 3, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 281, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (300.27, 111.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 336, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (352.28, 168.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 318, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (297.98, 109.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681330 reads in 216.799 CPU sec, 6.934 real sec
[M::mem_process_seqs] Processed 681476 reads in 162.632 CPU sec, 5.211 real sec
sorting block 11, #reads = 1072222 mem = 536879583 peak = 915648512
[M::mem_process_seqs] Processed 682514 reads in 178.901 CPU sec, 5.757 real sec
sorting block 12, #reads = 1069978 mem = 536878169 peak = 915648512
[M::process] read 680994 sequences (100000273 bp) in 2.129 CPU sec, 2.300 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 277881, 5, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 303, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (317.68, 180.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 342, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (358.51, 171.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 269, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595)
[M::mem_pestat] mean and std.dev: (250.47, 92.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 723)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680812 sequences (100000170 bp) in 1.622 CPU sec, 1.697 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 278126, 5, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (214, 254, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (292.06, 149.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 343, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (358.83, 169.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 214, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (245.35, 106.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681520 reads in 148.883 CPU sec, 4.748 real sec
[M::mem_process_seqs] Processed 680994 reads in 96.689 CPU sec, 3.052 real sec
[M::process] read 681024 sequences (100000081 bp) in 1.489 CPU sec, 1.546 real sec...
sorting block 13, #reads = 1069046 mem = 536877576 peak = 915648512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 277880, 2, 51)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 228, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055)
[M::mem_pestat] mean and std.dev: (228.55, 99.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1348)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 345, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (360.86, 170.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 356, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (342.42, 161.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682910 sequences (100000096 bp) in 1.844 CPU sec, 1.968 real sec...
[M::process] read 681298 sequences (100000104 bp) in 1.270 CPU sec, 1.263 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 278850, 4, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 244, 731)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1831)
[M::mem_pestat] mean and std.dev: (369.26, 313.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2381)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 349, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (364.53, 174.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 344, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (341.00, 168.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680812 reads in 146.086 CPU sec, 4.660 real sec
sorting block 14, #reads = 1069973 mem = 536878272 peak = 915648512
[M::mem_process_seqs] Processed 681024 reads in 94.051 CPU sec, 2.998 real sec
sorting block 15, #reads = 1068634 mem = 536877377 peak = 915648512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278584, 7, 60)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 314, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (281.71, 159.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 343, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (359.04, 173.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 311, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (302.61, 115.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680258 sequences (100000112 bp) in 2.016 CPU sec, 2.153 real sec...
[M::process] read 679804 sequences (100000157 bp) in 1.290 CPU sec, 1.285 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 278035, 5, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 276, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683)
[M::mem_pestat] mean and std.dev: (258.89, 125.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 353, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (366.69, 175.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 349, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (332.81, 123.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 965)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682910 reads in 143.438 CPU sec, 4.558 real sec
[M::mem_process_seqs] Processed 681298 reads in 92.574 CPU sec, 2.950 real sec
sorting block 16, #reads = 1071456 mem = 536877969 peak = 915648512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 277978, 3, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 292, 905)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2315)
[M::mem_pestat] mean and std.dev: (449.06, 357.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3020)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 360, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (373.07, 176.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 294, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (334.89, 164.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681384 sequences (100000240 bp) in 2.070 CPU sec, 2.214 real sec...
[M::process] read 681012 sequences (100000257 bp) in 1.320 CPU sec, 1.312 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 277880, 6, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 226, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (208.36, 98.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 366, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (378.82, 179.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 297, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620)
[M::mem_pestat] mean and std.dev: (295.00, 112.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 756)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680258 reads in 143.302 CPU sec, 4.558 real sec
[M::mem_process_seqs] Processed 679804 reads in 93.273 CPU sec, 2.968 real sec
sorting block 17, #reads = 1069346 mem = 536877849 peak = 915648512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 278307, 6, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 257, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (286.57, 144.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 352, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (367.21, 176.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 346, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (360.50, 168.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681092 sequences (100000223 bp) in 1.979 CPU sec, 2.133 real sec...
[M::process] read 681432 sequences (100000297 bp) in 1.330 CPU sec, 1.321 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 277775, 3, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (280, 375, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (421.03, 217.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1292)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 350, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (365.80, 175.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 302, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (325.70, 166.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681384 reads in 145.663 CPU sec, 4.634 real sec
[M::mem_process_seqs] Processed 681012 reads in 94.995 CPU sec, 3.019 real sec
sorting block 18, #reads = 1070453 mem = 536875675 peak = 915648512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 277793, 6, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (228, 308, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (282.63, 97.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 764)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 348, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (364.40, 174.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 370, 542)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1084)
[M::mem_pestat] mean and std.dev: (360.81, 149.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1355)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681342 sequences (100000009 bp) in 2.176 CPU sec, 2.304 real sec...
[M::process] read 681042 sequences (100000269 bp) in 1.330 CPU sec, 1.331 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 277858, 3, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 268, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (252.61, 114.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 343, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (359.73, 172.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 488, 641)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1379)
[M::mem_pestat] mean and std.dev: (431.97, 215.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1748)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681092 reads in 147.053 CPU sec, 4.675 real sec
sorting block 19, #reads = 1069917 mem = 536879349 peak = 915648512
[M::mem_process_seqs] Processed 681432 reads in 96.264 CPU sec, 3.060 real sec
sorting block 20, #reads = 1068555 mem = 536878939 peak = 942424064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 278121, 4, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 163, 631)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1605)
[M::mem_pestat] mean and std.dev: (299.46, 328.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2092)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 344, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (360.55, 173.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 315, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (278.67, 129.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680846 sequences (100000268 bp) in 2.017 CPU sec, 2.166 real sec...
[M::process] read 680784 sequences (100000244 bp) in 1.320 CPU sec, 1.318 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 277805, 7, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 254, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (280.12, 119.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 347, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (364.07, 175.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 302, 556)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1222)
[M::mem_pestat] mean and std.dev: (371.97, 203.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1555)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681342 reads in 146.390 CPU sec, 4.656 real sec
[M::mem_process_seqs] Processed 681042 reads in 94.606 CPU sec, 3.006 real sec
sorting block 21, #reads = 1069242 mem = 536879289 peak = 942424064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 278005, 3, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 283, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (278.25, 123.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 347, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (364.54, 175.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 228, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586)
[M::mem_pestat] mean and std.dev: (245.24, 130.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 769)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681562 sequences (100000001 bp) in 2.125 CPU sec, 2.241 real sec...
[M::process] read 681626 sequences (100000058 bp) in 1.310 CPU sec, 1.316 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 278188, 4, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 314, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1230)
[M::mem_pestat] mean and std.dev: (348.68, 285.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1591)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 349, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (366.17, 176.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 402, 704)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1696)
[M::mem_pestat] mean and std.dev: (421.86, 298.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2192)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680846 reads in 143.602 CPU sec, 4.572 real sec
[M::mem_process_seqs] Processed 680784 reads in 98.812 CPU sec, 3.157 real sec
sorting block 22, #reads = 1069590 mem = 536878755 peak = 942424064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 278177, 4, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 215, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (224.87, 108.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 345, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (362.17, 174.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 288, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (291.89, 128.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 972)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680534 sequences (100000080 bp) in 1.924 CPU sec, 2.038 real sec...
[M::process] read 679900 sequences (100000007 bp) in 1.300 CPU sec, 1.302 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 277188, 12, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 252, 536)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1224)
[M::mem_pestat] mean and std.dev: (276.82, 180.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1568)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 351, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (365.67, 176.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (553, 1070, 2612)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6730)
[M::mem_pestat] mean and std.dev: (1382.75, 1274.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8789)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 257, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (315.19, 190.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681562 reads in 145.023 CPU sec, 4.626 real sec
sorting block 23, #reads = 1069674 mem = 536879559 peak = 942424064
[M::mem_process_seqs] Processed 681626 reads in 101.839 CPU sec, 3.255 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 277190, 5, 48)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 303, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (295.42, 133.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 358, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (373.15, 178.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 291, 545)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1197)
[M::mem_pestat] mean and std.dev: (271.95, 116.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1523)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 679832 sequences (100000112 bp) in 2.038 CPU sec, 2.164 real sec...
[M::process] read 681040 sequences (100000229 bp) in 1.320 CPU sec, 1.321 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 277706, 11, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 223, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (235.00, 101.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 741)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 363, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (376.57, 178.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (404, 422, 1391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3365)
[M::mem_pestat] mean and std.dev: (788.64, 566.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4352)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 247, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (265.50, 122.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1085)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680534 reads in 145.936 CPU sec, 4.655 real sec
sorting block 24, #reads = 1071005 mem = 536880967 peak = 942424064
[M::mem_process_seqs] Processed 679900 reads in 100.542 CPU sec, 3.213 real sec
sorting block 25, #reads = 1071403 mem = 536876935 peak = 984518656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 277421, 5, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 361, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1171)
[M::mem_pestat] mean and std.dev: (406.38, 269.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1503)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 365, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (378.71, 179.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 380, 587)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1323)
[M::mem_pestat] mean and std.dev: (422.93, 294.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1691)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680956 sequences (100000102 bp) in 1.966 CPU sec, 2.075 real sec...
[M::process] read 681474 sequences (100000141 bp) in 1.300 CPU sec, 1.302 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 277940, 1, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (205, 312, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (283.43, 117.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 877)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 353, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (369.08, 177.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 277, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (305.85, 123.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 968)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 679832 reads in 146.854 CPU sec, 4.679 real sec
[M::mem_process_seqs] Processed 681040 reads in 95.087 CPU sec, 3.024 real sec
sorting block 26, #reads = 1071291 mem = 536877790 peak = 984518656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 277596, 5, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 174, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (208.75, 125.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 350, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (366.26, 175.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 265, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (284.50, 123.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681068 sequences (100000138 bp) in 2.091 CPU sec, 2.203 real sec...
[M::process] read 681214 sequences (100000185 bp) in 1.320 CPU sec, 1.324 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 277632, 10, 56)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 209, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (218.29, 98.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 800)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 345, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (361.59, 173.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (647, 3414, 3907)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10427)
[M::mem_pestat] mean and std.dev: (2526.70, 1534.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 13687)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 314, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075)
[M::mem_pestat] mean and std.dev: (356.89, 210.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1371)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680956 reads in 149.620 CPU sec, 4.757 real sec
[M::mem_process_seqs] Processed 681474 reads in 95.729 CPU sec, 3.051 real sec
sorting block 27, #reads = 1069799 mem = 536878682 peak = 984518656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 277904, 6, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 202, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584)
[M::mem_pestat] mean and std.dev: (231.05, 91.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 714)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 346, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (361.28, 171.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 329, 1294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3458)
[M::mem_pestat] mean and std.dev: (717.19, 817.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4540)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680958 sequences (100000276 bp) in 2.156 CPU sec, 2.271 real sec...
[M::process] read 680322 sequences (100000130 bp) in 1.300 CPU sec, 1.300 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 277266, 6, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 254, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (256.12, 127.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 346, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (362.64, 173.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 302, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (308.84, 171.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681068 reads in 149.506 CPU sec, 4.756 real sec
sorting block 28, #reads = 1069869 mem = 536878607 peak = 984518656
[M::mem_process_seqs] Processed 681214 reads in 96.545 CPU sec, 3.070 real sec
sorting block 29, #reads = 1070626 mem = 536879329 peak = 1013383168
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 277892, 4, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 296, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (292.98, 169.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 346, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (362.46, 173.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (275, 310, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 491)
[M::mem_pestat] mean and std.dev: (290.44, 65.09)
[M::mem_pestat] low and high boundaries for proper pairs: (30, 563)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680040 sequences (100000164 bp) in 2.081 CPU sec, 2.195 real sec...
[M::process] read 684986 sequences (100000064 bp) in 1.360 CPU sec, 1.360 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 277824, 7, 76)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 269, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1156)
[M::mem_pestat] mean and std.dev: (307.14, 160.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1483)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 350, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (365.05, 172.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 449, 752)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1748)
[M::mem_pestat] mean and std.dev: (453.36, 244.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2246)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680958 reads in 147.635 CPU sec, 4.691 real sec
[M::mem_process_seqs] Processed 680322 reads in 94.435 CPU sec, 3.002 real sec
sorting block 30, #reads = 1069911 mem = 536879500 peak = 1013383168
[M::process] read 685790 sequences (100000106 bp) in 2.271 CPU sec, 2.401 real sec...
[M::process] read 683902 sequences (100000205 bp) in 1.330 CPU sec, 1.336 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 279504, 2, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 221, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (247.15, 142.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 329, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (348.93, 172.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 261, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (266.00, 125.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684986 reads in 84.537 CPU sec, 2.675 real sec
sorting block 31, #reads = 1069727 mem = 536879941 peak = 1013383168
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 277882, 9, 59)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 262, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (294.09, 133.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 353, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (368.16, 173.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 279, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (309.25, 145.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 279985, 5, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 206, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062)
[M::mem_pestat] mean and std.dev: (258.07, 143.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1364)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 326, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (345.89, 171.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 341, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (312.26, 122.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683686 sequences (100000270 bp) in 1.649 CPU sec, 1.656 real sec...
[M::mem_process_seqs] Processed 680040 reads in 233.712 CPU sec, 7.423 real sec
[M::mem_process_seqs] Processed 685790 reads in 116.682 CPU sec, 3.722 real sec
sorting block 32, #reads = 1069633 mem = 536877383 peak = 1013383168
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 279222, 8, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 266, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (284.74, 127.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 339, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (353.07, 170.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 234, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (265.17, 135.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685990 sequences (100000070 bp) in 2.407 CPU sec, 2.559 real sec...
[M::process] read 685898 sequences (100000132 bp) in 1.370 CPU sec, 1.367 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 279163, 12, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 204, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610)
[M::mem_pestat] mean and std.dev: (233.08, 88.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 753)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 339, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (354.22, 170.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1037, 1247, 1507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (97, 2447)
[M::mem_pestat] mean and std.dev: (1104.50, 276.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2917)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 257, 582)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1422)
[M::mem_pestat] mean and std.dev: (378.03, 318.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1842)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683902 reads in 152.496 CPU sec, 4.846 real sec
[M::mem_process_seqs] Processed 683686 reads in 98.149 CPU sec, 3.118 real sec
sorting block 33, #reads = 1069055 mem = 536879136 peak = 1013383168
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 280125, 7, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (201, 273, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (315.64, 150.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 323, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (341.28, 167.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 313, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (312.43, 124.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 861)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683694 sequences (100000030 bp) in 1.943 CPU sec, 2.090 real sec...
[M::process] read 686188 sequences (100000096 bp) in 1.420 CPU sec, 1.418 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 279748, 5, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 301, 738)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1850)
[M::mem_pestat] mean and std.dev: (389.38, 333.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2406)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 324, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (341.13, 166.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 336, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879)
[M::mem_pestat] mean and std.dev: (329.13, 156.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685990 reads in 162.155 CPU sec, 5.163 real sec
[M::mem_process_seqs] Processed 685898 reads in 106.717 CPU sec, 3.399 real sec
sorting block 34, #reads = 1068740 mem = 536879640 peak = 1036623872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278555, 8, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 229, 265)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 439)
[M::mem_pestat] mean and std.dev: (209.05, 57.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 526)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 334, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (349.65, 168.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 293, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (291.30, 117.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683674 sequences (100000168 bp) in 2.220 CPU sec, 2.333 real sec...
[M::process] read 683314 sequences (100000059 bp) in 1.400 CPU sec, 1.392 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 279843, 8, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (225, 257, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (83, 438)
[M::mem_pestat] mean and std.dev: (242.43, 67.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 512)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 320, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (335.84, 162.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 241, 557)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1313)
[M::mem_pestat] mean and std.dev: (309.65, 227.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1691)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683694 reads in 167.397 CPU sec, 5.326 real sec
sorting block 35, #reads = 1064916 mem = 536877183 peak = 1036623872
[M::mem_process_seqs] Processed 686188 reads in 114.144 CPU sec, 3.644 real sec
sorting block 36, #reads = 1061756 mem = 536878301 peak = 1061650432
[M::process] read 685058 sequences (100000113 bp) in 1.852 CPU sec, 1.957 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 278895, 8, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 216, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1217)
[M::mem_pestat] mean and std.dev: (337.02, 276.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1573)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 332, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (347.59, 166.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 341, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062)
[M::mem_pestat] mean and std.dev: (348.25, 206.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1349)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 688882 sequences (100000264 bp) in 1.448 CPU sec, 1.444 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 279052, 5, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (196, 306, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (298.89, 136.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 336, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (352.14, 169.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 280, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1086)
[M::mem_pestat] mean and std.dev: (368.64, 170.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1370)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 279995, 7, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 288, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (325.40, 196.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 325, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (345.17, 170.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 348, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (327.54, 163.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1307)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683674 reads in 238.857 CPU sec, 7.636 real sec
[M::mem_process_seqs] Processed 683314 reads in 180.757 CPU sec, 5.799 real sec
[M::mem_process_seqs] Processed 685058 reads in 199.389 CPU sec, 6.411 real sec
sorting block 37, #reads = 1062269 mem = 536877613 peak = 1061650432
[M::process] read 685986 sequences (100000136 bp) in 2.408 CPU sec, 2.562 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 280983, 10, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 233, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (227.50, 96.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 743)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 307, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (327.76, 163.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (618, 2474, 7180)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 20304)
[M::mem_pestat] mean and std.dev: (3012.40, 2853.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 26866)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 258, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (257.70, 111.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 38, #reads = 1062091 mem = 536883104 peak = 1061650432
[M::process] read 684658 sequences (100000167 bp) in 1.702 CPU sec, 1.791 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 280256, 1, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 309, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1135)
[M::mem_pestat] mean and std.dev: (318.17, 209.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1465)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 322, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (339.36, 165.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 234, 254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (158, 318)
[M::mem_pestat] mean and std.dev: (229.87, 30.22)
[M::mem_pestat] low and high boundaries for proper pairs: (109, 351)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685308 sequences (100000129 bp) in 1.320 CPU sec, 1.324 real sec...
[M::mem_process_seqs] Processed 688882 reads in 170.903 CPU sec, 5.442 real sec
[M::mem_process_seqs] Processed 685986 reads in 110.583 CPU sec, 3.758 real sec
sorting block 39, #reads = 1062046 mem = 536882286 peak = 1061650432
[M::process] read 685056 sequences (100000116 bp) in 2.003 CPU sec, 2.126 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 280106, 2, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 257, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (241.96, 104.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 332, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (347.17, 167.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 392, 587)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1301)
[M::mem_pestat] mean and std.dev: (368.35, 184.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1658)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684658 reads in 89.771 CPU sec, 3.151 real sec
[M::process] read 685096 sequences (100000000 bp) in 1.860 CPU sec, 1.964 real sec...
sorting block 40, #reads = 1064915 mem = 536878413 peak = 1061650432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 279674, 10, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 244, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (277.79, 143.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 327, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (344.52, 168.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 355, 590)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1428)
[M::mem_pestat] mean and std.dev: (264.88, 147.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1847)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 264, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771)
[M::mem_pestat] mean and std.dev: (260.74, 87.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 965)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 684766 sequences (100000022 bp) in 1.386 CPU sec, 1.395 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 280540, 10, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (269, 451, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (320.41, 125.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 325, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (342.58, 167.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (590, 1386, 1565)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3515)
[M::mem_pestat] mean and std.dev: (1004.25, 430.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4490)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 263, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (287.79, 91.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 966)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685056 reads in 177.356 CPU sec, 5.688 real sec
[M::mem_process_seqs] Processed 685308 reads in 189.710 CPU sec, 6.154 real sec
sorting block 41, #reads = 1068083 mem = 536877995 peak = 1061650432
[M::process] read 682556 sequences (100000079 bp) in 1.979 CPU sec, 2.088 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 279421, 11, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 305, 552)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1272)
[M::mem_pestat] mean and std.dev: (321.51, 217.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1632)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 326, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (344.11, 168.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (445, 1438, 1779)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4447)
[M::mem_pestat] mean and std.dev: (1182.55, 957.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5781)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 206, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (232.91, 115.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 968)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685096 reads in 95.405 CPU sec, 3.155 real sec
sorting block 42, #reads = 1065259 mem = 536881552 peak = 1061650432
[M::process] read 681880 sequences (100000206 bp) in 1.722 CPU sec, 1.983 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 279140, 5, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 290, 699)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1737)
[M::mem_pestat] mean and std.dev: (368.09, 369.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2256)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 328, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (346.75, 170.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 323, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1127)
[M::mem_pestat] mean and std.dev: (311.13, 148.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1440)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681356 sequences (100000259 bp) in 1.420 CPU sec, 1.411 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 278545, 8, 50)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 322, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (318.07, 158.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 343, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (361.38, 176.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 252, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (282.55, 155.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684766 reads in 190.436 CPU sec, 6.112 real sec
[M::mem_process_seqs] Processed 682556 reads in 203.482 CPU sec, 6.629 real sec
sorting block 43, #reads = 1062896 mem = 536880383 peak = 1061650432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 278899, 3, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 213, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1187)
[M::mem_pestat] mean and std.dev: (298.48, 219.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1532)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 351, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (368.75, 180.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1317)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (275, 366, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (339.81, 128.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685166 sequences (100000228 bp) in 2.248 CPU sec, 2.423 real sec...
[M::process] read 683824 sequences (100000228 bp) in 1.470 CPU sec, 1.476 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 278506, 3, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 291, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (268.90, 105.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 352, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045)
[M::mem_pestat] mean and std.dev: (370.94, 182.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1319)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 245, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1232)
[M::mem_pestat] mean and std.dev: (267.11, 159.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1591)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681880 reads in 165.954 CPU sec, 5.366 real sec
[M::mem_process_seqs] Processed 681356 reads in 105.588 CPU sec, 3.440 real sec
sorting block 44, #reads = 1067217 mem = 536878180 peak = 1061650432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 279107, 20, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (224, 264, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734)
[M::mem_pestat] mean and std.dev: (287.24, 118.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 330, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (352.33, 178.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (537, 1353, 1353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2985)
[M::mem_pestat] mean and std.dev: (1194.30, 879.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4713)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 318, 539)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1279)
[M::mem_pestat] mean and std.dev: (317.59, 177.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1649)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 682036 sequences (100000151 bp) in 2.174 CPU sec, 2.316 real sec...
[M::process] read 681946 sequences (100000040 bp) in 1.380 CPU sec, 1.384 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 280036, 8, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 239, 587)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1491)
[M::mem_pestat] mean and std.dev: (366.62, 336.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1943)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 340, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (359.06, 178.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 386, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1096)
[M::mem_pestat] mean and std.dev: (342.39, 185.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1400)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685166 reads in 164.853 CPU sec, 5.343 real sec
[M::mem_process_seqs] Processed 683824 reads in 100.100 CPU sec, 3.252 real sec
sorting block 45, #reads = 1063274 mem = 536880201 peak = 1061650432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 279739, 1, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 284, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (311.85, 175.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 350, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (365.65, 177.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 253, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (298.84, 191.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684202 sequences (100000180 bp) in 2.014 CPU sec, 2.178 real sec...
[M::process] read 683156 sequences (100000169 bp) in 1.520 CPU sec, 1.523 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278639, 12, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 326, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (252.50, 95.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 355, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (369.89, 179.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1300)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1286, 1754, 1926)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (6, 3206)
[M::mem_pestat] mean and std.dev: (1431.33, 769.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4510)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 264, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (255.67, 117.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682036 reads in 153.453 CPU sec, 4.957 real sec
sorting block 46, #reads = 1070408 mem = 536879294 peak = 1061650432
[M::mem_process_seqs] Processed 681946 reads in 95.110 CPU sec, 3.092 real sec
sorting block 47, #reads = 1070018 mem = 536878356 peak = 1061650432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 279555, 8, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 210, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (219.47, 128.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 334, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (353.83, 175.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 267, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (281.70, 104.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 897)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682390 sequences (100000155 bp) in 2.308 CPU sec, 2.460 real sec...
[M::process] read 682438 sequences (100000070 bp) in 1.538 CPU sec, 1.544 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 279618, 11, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 331, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (287.25, 141.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 343, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (361.64, 178.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1296)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (99, 1061, 2400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7002)
[M::mem_pestat] mean and std.dev: (1100.36, 988.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9303)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 399, 540)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1208)
[M::mem_pestat] mean and std.dev: (356.64, 170.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1542)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684202 reads in 157.905 CPU sec, 5.096 real sec
[M::mem_process_seqs] Processed 683156 reads in 100.162 CPU sec, 3.268 real sec
sorting block 48, #reads = 1070860 mem = 536886531 peak = 1061650432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278760, 0, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (228, 355, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (313.53, 125.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1092)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 348, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (366.10, 180.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1300)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 294, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (300.61, 148.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682366 sequences (100000072 bp) in 2.114 CPU sec, 2.237 real sec...
[M::process] read 682516 sequences (100000260 bp) in 1.560 CPU sec, 1.560 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 278707, 5, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (208, 316, 592)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1360)
[M::mem_pestat] mean and std.dev: (390.69, 250.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1744)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 343, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (363.31, 179.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 276, 674)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1596)
[M::mem_pestat] mean and std.dev: (351.00, 266.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2057)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682390 reads in 161.667 CPU sec, 5.223 real sec
[M::mem_process_seqs] Processed 682438 reads in 102.926 CPU sec, 3.335 real sec
sorting block 49, #reads = 1069459 mem = 536879867 peak = 1061650432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278871, 3, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 322, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (321.41, 185.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 343, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (364.31, 181.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1298)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 301, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601)
[M::mem_pestat] mean and std.dev: (268.75, 69.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 725)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682666 sequences (100000263 bp) in 2.331 CPU sec, 2.485 real sec...
[M::process] read 684908 sequences (100000164 bp) in 1.580 CPU sec, 1.582 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 278801, 5, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 264, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (324.11, 208.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1327)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 343, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (364.37, 181.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1305)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 266, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (275.58, 116.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682366 reads in 164.881 CPU sec, 5.321 real sec
sorting block 50, #reads = 1066637 mem = 536879738 peak = 1061650432
[M::mem_process_seqs] Processed 682516 reads in 104.189 CPU sec, 3.384 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 278691, 2, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 260, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (310.76, 195.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 341, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (364.47, 183.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1308)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 235, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549)
[M::mem_pestat] mean and std.dev: (240.76, 103.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 675)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 51, #reads = 1067813 mem = 536883199 peak = 1061650432
[M::process] read 685884 sequences (100000226 bp) in 2.257 CPU sec, 2.376 real sec...
[M::process] read 684006 sequences (100000035 bp) in 1.540 CPU sec, 1.544 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 279929, 1, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (245, 337, 613)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1349)
[M::mem_pestat] mean and std.dev: (377.45, 197.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1717)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 328, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (352.46, 179.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 212, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (249.36, 109.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 877)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682666 reads in 171.847 CPU sec, 5.551 real sec
[M::mem_process_seqs] Processed 684908 reads in 114.236 CPU sec, 3.645 real sec
sorting block 52, #reads = 1064323 mem = 536880035 peak = 1061650432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 280264, 9, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 239, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601)
[M::mem_pestat] mean and std.dev: (264.83, 144.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 327, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (349.22, 176.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 489, 1012)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2530)
[M::mem_pestat] mean and std.dev: (559.16, 454.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3289)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682012 sequences (100000186 bp) in 2.241 CPU sec, 2.366 real sec...
[M::process] read 683356 sequences (100000022 bp) in 1.540 CPU sec, 1.540 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 280136, 10, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 289, 545)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1247)
[M::mem_pestat] mean and std.dev: (374.70, 286.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1598)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 341, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (357.87, 176.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (291, 887, 1108)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2742)
[M::mem_pestat] mean and std.dev: (663.67, 361.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3559)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 293, 563)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1231)
[M::mem_pestat] mean and std.dev: (370.48, 193.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1565)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685884 reads in 161.720 CPU sec, 5.154 real sec
[M::mem_process_seqs] Processed 684006 reads in 104.189 CPU sec, 3.591 real sec
sorting block 53, #reads = 1064933 mem = 536879577 peak = 1061650432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 278943, 5, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 246, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (301.07, 179.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1080)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 353, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (366.50, 175.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1291)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 369, 544)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1162)
[M::mem_pestat] mean and std.dev: (328.51, 157.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1471)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682304 sequences (100000168 bp) in 2.363 CPU sec, 2.510 real sec...
[M::process] read 682326 sequences (100000138 bp) in 1.540 CPU sec, 1.539 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 279508, 6, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 240, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (267.60, 138.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1303)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 340, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (356.98, 174.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 321, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (324.62, 130.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682012 reads in 163.372 CPU sec, 5.471 real sec
[M::mem_process_seqs] Processed 683356 reads in 102.383 CPU sec, 3.337 real sec
sorting block 54, #reads = 1065412 mem = 536879376 peak = 1061650432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278422, 5, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 381, 608)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1464)
[M::mem_pestat] mean and std.dev: (383.07, 239.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1892)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 343, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (359.71, 173.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 253, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (296.29, 179.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681824 sequences (100000029 bp) in 2.358 CPU sec, 2.501 real sec...
[M::process] read 681620 sequences (100000268 bp) in 1.490 CPU sec, 1.487 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 278405, 5, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 244, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (255.00, 156.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 339, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (354.94, 168.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 338, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1139)
[M::mem_pestat] mean and std.dev: (349.95, 155.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1447)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682304 reads in 167.287 CPU sec, 5.401 real sec
sorting block 55, #reads = 1067372 mem = 536878750 peak = 1061650432
[M::mem_process_seqs] Processed 682326 reads in 106.283 CPU sec, 3.373 real sec
sorting block 56, #reads = 1064630 mem = 536882072 peak = 1061650432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 278759, 8, 52)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 209, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.10, 120.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 339, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (356.70, 170.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 288, 547)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1191)
[M::mem_pestat] mean and std.dev: (322.36, 161.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1513)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681428 sequences (100000120 bp) in 2.339 CPU sec, 2.483 real sec...
[M::process] read 680950 sequences (100000199 bp) in 1.460 CPU sec, 1.464 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 277868, 10, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 265, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (268.95, 136.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 339, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (355.89, 170.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (433, 498, 1153)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2593)
[M::mem_pestat] mean and std.dev: (716.70, 645.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3313)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 226, 264)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454)
[M::mem_pestat] mean and std.dev: (210.24, 74.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 549)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681824 reads in 161.618 CPU sec, 5.215 real sec
[M::mem_process_seqs] Processed 681620 reads in 104.660 CPU sec, 3.341 real sec
sorting block 57, #reads = 1066060 mem = 536881086 peak = 1061650432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 277621, 6, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (222, 365, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (367.81, 191.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1310)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 338, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (356.77, 171.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 320, 534)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1192)
[M::mem_pestat] mean and std.dev: (415.33, 309.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1654)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680980 sequences (100000073 bp) in 2.134 CPU sec, 2.261 real sec...
[M::process] read 147820 sequences (21667793 bp) in 0.590 CPU sec, 0.586 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 278160, 7, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 237, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (275.05, 156.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 341, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (358.72, 171.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 199, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (232.85, 116.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681428 reads in 152.825 CPU sec, 4.900 real sec
[M::mem_process_seqs] Processed 680950 reads in 97.307 CPU sec, 3.269 real sec
sorting block 58, #reads = 1068190 mem = 536879353 peak = 1061650432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 277948, 4, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 262, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (262.21, 115.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 346, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (363.91, 175.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 269, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (271.19, 102.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 60667, 2, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (300, 433, 582)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1146)
[M::mem_pestat] mean and std.dev: (338.27, 125.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1428)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 339, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (358.40, 174.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 465, 1469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3991)
[M::mem_pestat] mean and std.dev: (743.42, 894.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5252)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 147820 reads in 34.051 CPU sec, 1.105 real sec
[M::mem_process_seqs] Processed 680980 reads in 102.430 CPU sec, 3.572 real sec
sorting block 59, #reads = 1068657 mem = 536879262 peak = 1136721920
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 64292060
overall: 70197821440 mem 9326.215 user 363.572 sys 625.484 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1029_N5D-T1-TDNA-1_A23MHWWLT4_1.L001\tPU:L001\tSM:1029_N5D-T1-TDNA-1_A23MHWWLT4_1\tLB:1029_N5D-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0004.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0004.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 625.521 sec; CPU: 9689.790 sec
sorting block 60, #reads = 570099 mem = 286833221 peak = 1136721920
uniq header count 1
merging 61 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3897750646
output reads: 64671419
bam_mem_sort: 61 calls 22.067 user 0.173 sys 22.419 real
bam_write: 61 calls 29.130 user 0.877 sys 30.220 real
execute: 1 calls 12.264 user 0.870 sys 647.174 real
merge_files: 1 calls 12.245 user 0.851 sys 20.965 real
parse_chunk: 969 calls 30.559 user 8.124 sys 39.448 real
read_chunk: 2617 calls 25.641 user 22.695 sys 265.946 real
sort_block: 1 calls 19.608 user 22.130 sys 257.086 real
write_chunk: 1646 calls 11.930 user 0.676 sys 20.257 real
overall: 2886230016 mem 794.941 user 39.478 sys 647.196 real
ls: cannot access '*.cram': No such file or directory