cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_d0M-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1638.451 CPU sec, 57.366 real sec
[M::process] read 679506 sequences (100000102 bp) in 1.186 CPU sec, 1.193 real sec...
[M::process] read 678660 sequences (100000025 bp) in 1.727 CPU sec, 1.745 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285302, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 299, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (321.93, 148.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677870 sequences (100000095 bp) in 1.768 CPU sec, 1.823 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285430, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 304, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (327.85, 151.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677786 sequences (100000118 bp) in 1.740 CPU sec, 1.800 real sec...
[M::mem_process_seqs] Processed 679506 reads in 182.298 CPU sec, 5.824 real sec
[M::mem_process_seqs] Processed 678660 reads in 120.285 CPU sec, 3.895 real sec
sorting block 0, #reads = 1081888 mem = 536877224 peak = 595369984
[M::process] read 677482 sequences (100000017 bp) in 2.148 CPU sec, 2.193 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284667, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 312, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865)
[M::mem_pestat] mean and std.dev: (332.84, 152.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1082)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677436 sequences (100000082 bp) in 1.830 CPU sec, 1.835 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285330, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 317, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (336.03, 152.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1096)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (587, 587, 587)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (587, 587)
[M::mem_pestat] mean and std.dev: (587.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (587, 587)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285372, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 320, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (339.68, 154.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677870 reads in 235.842 CPU sec, 7.584 real sec
[M::mem_process_seqs] Processed 677786 reads in 177.381 CPU sec, 5.741 real sec
[M::mem_process_seqs] Processed 677482 reads in 196.598 CPU sec, 6.380 real sec
sorting block 1, #reads = 1081132 mem = 536880695 peak = 730636288
[M::process] read 677192 sequences (100000000 bp) in 2.214 CPU sec, 2.319 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285500, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 320, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (339.07, 154.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 2, #reads = 1080869 mem = 536878030 peak = 938192896
[M::process] read 676880 sequences (100000239 bp) in 2.238 CPU sec, 2.280 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285080, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 324, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (342.77, 156.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677436 reads in 169.304 CPU sec, 5.395 real sec
[M::mem_process_seqs] Processed 677192 reads in 114.200 CPU sec, 3.604 real sec
[M::process] read 676956 sequences (100000007 bp) in 2.089 CPU sec, 2.119 real sec...
sorting block 3, #reads = 1082167 mem = 536878942 peak = 938192896
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285127, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 326, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (344.77, 156.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676482 sequences (100000210 bp) in 2.047 CPU sec, 2.374 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285047, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 326, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (344.92, 156.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676378 sequences (100000017 bp) in 1.939 CPU sec, 1.945 real sec...
[M::mem_process_seqs] Processed 676880 reads in 167.663 CPU sec, 5.384 real sec
[M::mem_process_seqs] Processed 676956 reads in 112.892 CPU sec, 3.598 real sec
sorting block 4, #reads = 1081315 mem = 536880038 peak = 938192896
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284657, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 329, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (346.87, 156.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (385, 2113, 2433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6529)
[M::mem_pestat] mean and std.dev: (1558.82, 902.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8577)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675962 sequences (100000254 bp) in 2.439 CPU sec, 2.646 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284735, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 331, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (348.81, 157.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676144 sequences (100000222 bp) in 1.964 CPU sec, 1.965 real sec...
[M::mem_process_seqs] Processed 676482 reads in 170.691 CPU sec, 5.453 real sec
sorting block 5, #reads = 1082600 mem = 536878740 peak = 938192896
[M::mem_process_seqs] Processed 676378 reads in 115.163 CPU sec, 3.669 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284337, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 331, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (348.43, 156.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676348 sequences (100000245 bp) in 2.562 CPU sec, 2.839 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284510, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 332, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (349.98, 157.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675908 sequences (100000278 bp) in 1.936 CPU sec, 1.952 real sec...
[M::mem_process_seqs] Processed 675962 reads in 171.156 CPU sec, 5.483 real sec
sorting block 6, #reads = 1081739 mem = 536879380 peak = 948260864
[M::mem_process_seqs] Processed 676144 reads in 115.042 CPU sec, 3.675 real sec
sorting block 7, #reads = 1081537 mem = 536878527 peak = 969928704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284355, 18, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 331, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (348.95, 157.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 2132, 2132)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6330)
[M::mem_pestat] mean and std.dev: (1384.11, 1162.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8429)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675724 sequences (100000246 bp) in 2.407 CPU sec, 2.570 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284258, 2, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 723, 723)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1865)
[M::mem_pestat] mean and std.dev: (581.30, 424.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2436)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 335, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (352.48, 159.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675690 sequences (100000301 bp) in 2.017 CPU sec, 2.025 real sec...
[M::mem_process_seqs] Processed 676348 reads in 172.576 CPU sec, 5.515 real sec
[M::mem_process_seqs] Processed 675908 reads in 116.020 CPU sec, 3.680 real sec
sorting block 8, #reads = 1080951 mem = 536879611 peak = 969928704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283894, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 334, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (350.98, 157.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675488 sequences (100000125 bp) in 2.685 CPU sec, 2.806 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283987, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 335, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (351.35, 156.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675300 sequences (100000041 bp) in 2.100 CPU sec, 2.109 real sec...
[M::mem_process_seqs] Processed 675724 reads in 175.034 CPU sec, 5.568 real sec
[M::mem_process_seqs] Processed 675690 reads in 117.136 CPU sec, 3.721 real sec
sorting block 9, #reads = 1080665 mem = 536881964 peak = 969928704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284048, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (284, 329, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (356.75, 107.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 336, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (351.98, 156.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675176 sequences (100000151 bp) in 2.297 CPU sec, 2.826 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284661, 4, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (97, 121, 161)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 289)
[M::mem_pestat] mean and std.dev: (107.80, 58.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 353)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 332, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (348.03, 152.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675488 reads in 171.192 CPU sec, 5.518 real sec
[M::process] read 675046 sequences (100000096 bp) in 2.141 CPU sec, 2.146 real sec...
[M::mem_process_seqs] Processed 675300 reads in 115.120 CPU sec, 3.682 real sec
sorting block 10, #reads = 1080408 mem = 536876347 peak = 969928704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283900, 3, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 335, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (350.70, 154.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (285, 415, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (25, 675)
[M::mem_pestat] mean and std.dev: (356.54, 133.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RR
[M::process] read 675296 sequences (100000227 bp) in 2.679 CPU sec, 3.219 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283840, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 334, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (350.71, 154.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675176 reads in 171.743 CPU sec, 5.527 real sec
sorting block 11, #reads = 1080669 mem = 536878861 peak = 969928704
[M::process] read 675220 sequences (100000257 bp) in 2.229 CPU sec, 2.285 real sec...
[M::mem_process_seqs] Processed 675046 reads in 116.886 CPU sec, 3.718 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283711, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 334, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (350.48, 155.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 455, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (386.64, 201.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RR
sorting block 12, #reads = 1079441 mem = 536877817 peak = 973209600
[M::process] read 675532 sequences (100000027 bp) in 2.441 CPU sec, 2.731 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283945, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 327, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (344.92, 151.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1095)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675296 reads in 174.596 CPU sec, 5.601 real sec
[M::process] read 675532 sequences (100000254 bp) in 2.011 CPU sec, 2.028 real sec...
[M::mem_process_seqs] Processed 675220 reads in 118.251 CPU sec, 3.760 real sec
sorting block 13, #reads = 1078169 mem = 536881759 peak = 973209600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284208, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 325, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (343.01, 151.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1093)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675126 sequences (100000099 bp) in 2.726 CPU sec, 2.979 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284480, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 326, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879)
[M::mem_pestat] mean and std.dev: (343.49, 151.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675532 reads in 174.124 CPU sec, 5.539 real sec
[M::process] read 675112 sequences (100000297 bp) in 2.129 CPU sec, 2.131 real sec...
[M::mem_process_seqs] Processed 675532 reads in 118.198 CPU sec, 3.746 real sec
sorting block 14, #reads = 1078945 mem = 536877315 peak = 973209600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284551, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 332, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (348.89, 153.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675300 sequences (100000200 bp) in 2.511 CPU sec, 2.772 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284040, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 334, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (351.56, 156.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675126 reads in 174.696 CPU sec, 5.606 real sec
[M::process] read 674954 sequences (100000086 bp) in 2.159 CPU sec, 2.183 real sec...
sorting block 15, #reads = 1077351 mem = 536878079 peak = 973209600
[M::mem_process_seqs] Processed 675112 reads in 118.502 CPU sec, 3.765 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284729, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 334, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (352.47, 157.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676240 sequences (100000053 bp) in 2.965 CPU sec, 3.181 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284444, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 336, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (354.48, 159.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675300 reads in 174.417 CPU sec, 5.584 real sec
sorting block 16, #reads = 1077458 mem = 536878246 peak = 973209600
[M::process] read 678886 sequences (100000292 bp) in 2.218 CPU sec, 2.240 real sec...
[M::mem_process_seqs] Processed 674954 reads in 118.623 CPU sec, 3.776 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284649, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 327, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (347.56, 159.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1272, 1836, 3434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7758)
[M::mem_pestat] mean and std.dev: (1926.85, 1159.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9920)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 17, #reads = 1078442 mem = 536885269 peak = 984760320
[M::process] read 678050 sequences (100000216 bp) in 2.485 CPU sec, 2.932 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284932, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 305, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863)
[M::mem_pestat] mean and std.dev: (328.83, 153.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1081)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676240 reads in 176.945 CPU sec, 5.736 real sec
[M::mem_process_seqs] Processed 678886 reads in 119.158 CPU sec, 3.808 real sec
[M::process] read 677454 sequences (100000284 bp) in 2.336 CPU sec, 2.395 real sec...
sorting block 18, #reads = 1078611 mem = 536879508 peak = 984760320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284672, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 311, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (334.10, 154.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1098)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677350 sequences (100000061 bp) in 2.892 CPU sec, 3.070 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284644, 3, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 317, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (337.30, 154.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1101)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (309, 309, 702)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1488)
[M::mem_pestat] mean and std.dev: (419.00, 197.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1881)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 678050 reads in 172.730 CPU sec, 5.524 real sec
[M::mem_process_seqs] Processed 677454 reads in 115.440 CPU sec, 3.650 real sec
[M::process] read 677338 sequences (100000245 bp) in 2.238 CPU sec, 2.297 real sec...
sorting block 19, #reads = 1083255 mem = 536879583 peak = 984760320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284370, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 317, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (336.49, 152.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677274 sequences (100000024 bp) in 2.851 CPU sec, 3.124 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284444, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 318, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (337.83, 153.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1105)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677350 reads in 172.305 CPU sec, 5.523 real sec
[M::mem_process_seqs] Processed 677338 reads in 115.742 CPU sec, 3.670 real sec
sorting block 20, #reads = 1082575 mem = 536879117 peak = 984760320
[M::process] read 676880 sequences (100000174 bp) in 2.435 CPU sec, 2.488 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285009, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 321, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (339.15, 154.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 21, #reads = 1082989 mem = 536881691 peak = 989880320
[M::process] read 676762 sequences (100000247 bp) in 2.681 CPU sec, 3.013 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284750, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 324, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (343.26, 156.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677274 reads in 166.885 CPU sec, 5.393 real sec
[M::mem_process_seqs] Processed 676880 reads in 112.994 CPU sec, 3.628 real sec
[M::process] read 676768 sequences (100000183 bp) in 2.323 CPU sec, 2.462 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284475, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 327, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (345.54, 157.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 22, #reads = 1081961 mem = 536878115 peak = 989880320
[M::process] read 676508 sequences (100000032 bp) in 2.566 CPU sec, 2.743 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285267, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 326, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (344.51, 156.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 307, 886)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2206)
[M::mem_pestat] mean and std.dev: (549.50, 439.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2866)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 676762 reads in 167.985 CPU sec, 5.465 real sec
[M::mem_process_seqs] Processed 676768 reads in 113.760 CPU sec, 3.664 real sec
[M::process] read 676396 sequences (100000082 bp) in 2.299 CPU sec, 2.578 real sec...
sorting block 23, #reads = 1081355 mem = 536877041 peak = 989880320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284795, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 326, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (344.15, 154.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676118 sequences (100000215 bp) in 2.617 CPU sec, 2.929 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284870, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 326, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (344.17, 154.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676508 reads in 167.799 CPU sec, 5.399 real sec
[M::mem_process_seqs] Processed 676396 reads in 111.662 CPU sec, 3.539 real sec
[M::process] read 676076 sequences (100000079 bp) in 2.444 CPU sec, 2.530 real sec...
sorting block 24, #reads = 1082713 mem = 536878425 peak = 989880320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284736, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 328, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (346.28, 155.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676046 sequences (100000145 bp) in 2.344 CPU sec, 2.645 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284480, 1, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 329, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (347.08, 155.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 235, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (143, 298)
[M::mem_pestat] mean and std.dev: (218.00, 34.63)
[M::mem_pestat] low and high boundaries for proper pairs: (79, 357)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 676118 reads in 170.412 CPU sec, 5.508 real sec
[M::mem_process_seqs] Processed 676076 reads in 116.397 CPU sec, 3.688 real sec
sorting block 25, #reads = 1082132 mem = 536879363 peak = 989880320
[M::process] read 676130 sequences (100000000 bp) in 2.279 CPU sec, 2.331 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283985, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 332, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (349.34, 157.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 26, #reads = 1081789 mem = 536879993 peak = 1019285504
[M::process] read 676016 sequences (100000201 bp) in 2.482 CPU sec, 2.764 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284474, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 331, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (349.02, 157.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (135, 242, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582)
[M::mem_pestat] mean and std.dev: (195.50, 60.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 731)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 676046 reads in 171.232 CPU sec, 5.512 real sec
[M::process] read 675910 sequences (100000174 bp) in 2.008 CPU sec, 2.068 real sec...
[M::mem_process_seqs] Processed 676130 reads in 115.456 CPU sec, 3.709 real sec
sorting block 27, #reads = 1081216 mem = 536878373 peak = 1019285504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284679, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 332, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (349.96, 158.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675738 sequences (100000035 bp) in 2.425 CPU sec, 2.684 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284379, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 332, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (349.12, 155.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676016 reads in 173.236 CPU sec, 5.554 real sec
[M::process] read 675480 sequences (100000001 bp) in 2.107 CPU sec, 2.116 real sec...
[M::mem_process_seqs] Processed 675910 reads in 116.736 CPU sec, 3.718 real sec
sorting block 28, #reads = 1081411 mem = 536876609 peak = 1019285504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284889, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 331, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (348.61, 155.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675376 sequences (100000063 bp) in 2.990 CPU sec, 3.333 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284063, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 333, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (350.98, 157.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675738 reads in 174.124 CPU sec, 5.567 real sec
[M::process] read 675566 sequences (100000145 bp) in 2.197 CPU sec, 2.210 real sec...
[M::mem_process_seqs] Processed 675480 reads in 117.304 CPU sec, 3.722 real sec
sorting block 29, #reads = 1081690 mem = 536878641 peak = 1019285504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284760, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 330, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (347.89, 155.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675988 sequences (100000259 bp) in 2.327 CPU sec, 2.632 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284855, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 331, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (348.63, 155.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 351, 1622)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4470)
[M::mem_pestat] mean and std.dev: (744.58, 907.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5894)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675376 reads in 170.227 CPU sec, 5.462 real sec
[M::process] read 675616 sequences (100000061 bp) in 2.115 CPU sec, 2.122 real sec...
sorting block 30, #reads = 1079772 mem = 536879467 peak = 1030815744
[M::mem_process_seqs] Processed 675566 reads in 115.222 CPU sec, 3.663 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284462, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 327, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (346.51, 156.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (316, 362, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865)
[M::mem_pestat] mean and std.dev: (436.25, 184.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RR
sorting block 31, #reads = 1080217 mem = 536881798 peak = 1030815744
[M::process] read 675632 sequences (100000156 bp) in 2.709 CPU sec, 3.027 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284897, 4, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (246, 246, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (268.00, 104.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 328, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (346.58, 155.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (277, 311, 844)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1978)
[M::mem_pestat] mean and std.dev: (368.38, 203.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2545)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675988 reads in 169.393 CPU sec, 5.423 real sec
[M::process] read 675490 sequences (100000193 bp) in 2.273 CPU sec, 2.296 real sec...
[M::mem_process_seqs] Processed 675616 reads in 114.838 CPU sec, 3.650 real sec
sorting block 32, #reads = 1079565 mem = 536878664 peak = 1030815744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284429, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 325, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (343.97, 153.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675438 sequences (100000012 bp) in 2.655 CPU sec, 2.818 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285269, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 329, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (347.34, 155.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675632 reads in 168.735 CPU sec, 5.411 real sec
[M::process] read 675480 sequences (100000278 bp) in 2.218 CPU sec, 2.237 real sec...
[M::mem_process_seqs] Processed 675490 reads in 113.910 CPU sec, 3.613 real sec
sorting block 33, #reads = 1079309 mem = 536877706 peak = 1030815744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284501, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 329, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (347.85, 156.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675332 sequences (100000059 bp) in 2.788 CPU sec, 3.053 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284646, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 332, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (350.58, 157.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675438 reads in 167.681 CPU sec, 5.392 real sec
[M::mem_process_seqs] Processed 675480 reads in 113.564 CPU sec, 3.616 real sec
[M::process] read 675152 sequences (100000076 bp) in 2.273 CPU sec, 2.325 real sec...
sorting block 34, #reads = 1080926 mem = 536878321 peak = 1030815744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285320, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 389, 1010)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2610)
[M::mem_pestat] mean and std.dev: (485.20, 353.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3410)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 335, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (353.81, 160.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 677386 sequences (100000227 bp) in 2.341 CPU sec, 2.855 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284628, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 337, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (355.32, 160.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675332 reads in 166.843 CPU sec, 5.394 real sec
[M::mem_process_seqs] Processed 675152 reads in 114.930 CPU sec, 3.667 real sec
sorting block 35, #reads = 1080552 mem = 536878357 peak = 1030815744
[M::process] read 678874 sequences (100000077 bp) in 2.363 CPU sec, 2.434 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285280, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 316, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (337.88, 156.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 36, #reads = 1079524 mem = 536876622 peak = 1036107776
[M::process] read 677806 sequences (100000042 bp) in 2.671 CPU sec, 3.026 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286125, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 303, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (326.39, 151.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677386 reads in 169.697 CPU sec, 5.484 real sec
[M::mem_process_seqs] Processed 678874 reads in 114.148 CPU sec, 3.674 real sec
[M::process] read 677292 sequences (100000158 bp) in 2.308 CPU sec, 2.383 real sec...
sorting block 37, #reads = 1080818 mem = 536878690 peak = 1036107776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285789, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 313, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872)
[M::mem_pestat] mean and std.dev: (334.83, 153.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1091)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676974 sequences (100000221 bp) in 2.504 CPU sec, 2.812 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 285242, 2, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (117, 143, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (162.06, 61.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 745)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 318, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881)
[M::mem_pestat] mean and std.dev: (338.57, 155.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 174, 6548)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 19350)
[M::mem_pestat] mean and std.dev: (2287.30, 3230.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 25751)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 677806 reads in 166.745 CPU sec, 5.385 real sec
[M::mem_process_seqs] Processed 677292 reads in 114.177 CPU sec, 3.633 real sec
[M::process] read 676788 sequences (100000254 bp) in 2.308 CPU sec, 2.340 real sec...
sorting block 38, #reads = 1082755 mem = 536877788 peak = 1036107776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285086, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 322, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (340.80, 154.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676556 sequences (100000109 bp) in 2.661 CPU sec, 2.953 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285489, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 323, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (342.44, 155.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676974 reads in 166.092 CPU sec, 5.350 real sec
[M::mem_process_seqs] Processed 676788 reads in 111.376 CPU sec, 3.546 real sec
[M::process] read 676370 sequences (100000194 bp) in 2.296 CPU sec, 2.338 real sec...
sorting block 39, #reads = 1082678 mem = 536879710 peak = 1036107776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285021, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 326, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (346.60, 158.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676294 sequences (100000219 bp) in 2.423 CPU sec, 2.834 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284729, 5, 20)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 329, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (348.35, 158.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (2416, 3378, 3378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (492, 5302)
[M::mem_pestat] mean and std.dev: (3019.88, 669.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6264)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 676556 reads in 163.693 CPU sec, 5.290 real sec
[M::mem_process_seqs] Processed 676370 reads in 112.741 CPU sec, 3.601 real sec
sorting block 40, #reads = 1082083 mem = 536878033 peak = 1036107776
[M::process] read 676174 sequences (100000132 bp) in 2.324 CPU sec, 2.377 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285239, 4, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (102, 718, 1012)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2832)
[M::mem_pestat] mean and std.dev: (380.38, 348.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3742)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (349.34, 159.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 41, #reads = 1081168 mem = 536884399 peak = 1039462400
[M::process] read 676110 sequences (100000232 bp) in 2.610 CPU sec, 2.854 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284642, 9, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 333, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (351.80, 161.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (461, 624, 684)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (15, 1130)
[M::mem_pestat] mean and std.dev: (484.00, 163.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1353)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 676294 reads in 164.446 CPU sec, 5.326 real sec
[M::mem_process_seqs] Processed 676174 reads in 113.971 CPU sec, 3.654 real sec
[M::process] read 675888 sequences (100000070 bp) in 2.241 CPU sec, 2.375 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284976, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 333, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (351.78, 160.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 42, #reads = 1082115 mem = 536893335 peak = 1039462400
[M::process] read 675814 sequences (100000103 bp) in 2.543 CPU sec, 2.765 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284876, 7, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 278, 699)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1767)
[M::mem_pestat] mean and std.dev: (391.90, 256.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2301)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 335, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (353.31, 160.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 676110 reads in 163.879 CPU sec, 5.301 real sec
[M::mem_process_seqs] Processed 675888 reads in 111.715 CPU sec, 3.560 real sec
[M::process] read 676432 sequences (100000276 bp) in 2.441 CPU sec, 2.522 real sec...
sorting block 43, #reads = 1081493 mem = 536878573 peak = 1039462400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284982, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 335, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (353.87, 160.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675620 sequences (100000109 bp) in 2.372 CPU sec, 2.650 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285363, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 325, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (346.83, 160.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675814 reads in 166.922 CPU sec, 5.396 real sec
[M::mem_process_seqs] Processed 676432 reads in 114.124 CPU sec, 3.653 real sec
[M::process] read 675512 sequences (100000105 bp) in 2.331 CPU sec, 2.392 real sec...
sorting block 44, #reads = 1081260 mem = 536877901 peak = 1039462400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284768, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 335, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (354.45, 161.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675498 sequences (100000115 bp) in 2.513 CPU sec, 2.836 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284957, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 336, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (355.18, 161.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675620 reads in 167.234 CPU sec, 5.402 real sec
sorting block 45, #reads = 1081715 mem = 536877308 peak = 1039462400
[M::mem_process_seqs] Processed 675512 reads in 114.596 CPU sec, 3.638 real sec
[M::process] read 675230 sequences (100000095 bp) in 2.192 CPU sec, 2.272 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284891, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 333, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (350.89, 157.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 46, #reads = 1080733 mem = 536879478 peak = 1065148416
[M::process] read 675096 sequences (100000024 bp) in 2.589 CPU sec, 2.917 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 284610, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (216, 493, 1733)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4767)
[M::mem_pestat] mean and std.dev: (752.47, 622.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6284)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 336, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (354.91, 160.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675498 reads in 167.064 CPU sec, 5.360 real sec
[M::process] read 675362 sequences (100000230 bp) in 2.081 CPU sec, 2.097 real sec...
[M::mem_process_seqs] Processed 675230 reads in 113.493 CPU sec, 3.606 real sec
sorting block 47, #reads = 1080717 mem = 536881922 peak = 1065148416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284395, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 334, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (352.69, 158.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675630 sequences (100000224 bp) in 2.558 CPU sec, 2.747 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284690, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 332, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (351.09, 158.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675096 reads in 165.855 CPU sec, 5.318 real sec
[M::process] read 675760 sequences (100000271 bp) in 2.188 CPU sec, 2.213 real sec...
[M::mem_process_seqs] Processed 675362 reads in 113.162 CPU sec, 3.582 real sec
sorting block 48, #reads = 1080632 mem = 536879782 peak = 1065148416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284858, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 330, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (350.60, 159.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675922 sequences (100000273 bp) in 2.632 CPU sec, 2.923 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284438, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 328, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (348.08, 157.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675630 reads in 170.457 CPU sec, 5.482 real sec
[M::process] read 675642 sequences (100000170 bp) in 2.133 CPU sec, 2.145 real sec...
[M::mem_process_seqs] Processed 675760 reads in 115.573 CPU sec, 3.670 real sec
sorting block 49, #reads = 1080243 mem = 536877277 peak = 1065148416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285090, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 328, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (349.33, 160.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675494 sequences (100000087 bp) in 2.471 CPU sec, 2.833 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284841, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 330, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (350.36, 159.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675922 reads in 166.484 CPU sec, 5.387 real sec
sorting block 50, #reads = 1080342 mem = 536878545 peak = 1068728320
[M::process] read 675276 sequences (100000235 bp) in 2.243 CPU sec, 2.273 real sec...
[M::mem_process_seqs] Processed 675642 reads in 112.840 CPU sec, 3.601 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284508, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 331, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (349.95, 158.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 51, #reads = 1080602 mem = 536878951 peak = 1078013952
[M::process] read 675224 sequences (100000199 bp) in 2.544 CPU sec, 3.025 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285114, 2, 18)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 332, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (351.09, 158.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 265, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 381)
[M::mem_pestat] mean and std.dev: (239.14, 45.65)
[M::mem_pestat] low and high boundaries for proper pairs: (57, 431)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675494 reads in 165.875 CPU sec, 5.354 real sec
[M::mem_process_seqs] Processed 675276 reads in 114.410 CPU sec, 3.631 real sec
[M::process] read 675290 sequences (100000220 bp) in 2.401 CPU sec, 2.477 real sec...
sorting block 52, #reads = 1079671 mem = 536877551 peak = 1078013952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284318, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 333, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (351.44, 158.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675150 sequences (100000297 bp) in 2.461 CPU sec, 2.802 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284788, 3, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (275, 275, 1303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3359)
[M::mem_pestat] mean and std.dev: (697.00, 584.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4387)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 336, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (354.55, 160.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675224 reads in 165.711 CPU sec, 5.352 real sec
[M::mem_process_seqs] Processed 675290 reads in 113.282 CPU sec, 3.614 real sec
[M::process] read 676022 sequences (100000105 bp) in 2.462 CPU sec, 2.501 real sec...
sorting block 53, #reads = 1078881 mem = 536879277 peak = 1078013952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285012, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 338, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (357.49, 163.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679206 sequences (100000201 bp) in 2.653 CPU sec, 2.985 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284834, 5, 19)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 332, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (352.42, 161.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (495, 495, 1106)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2328)
[M::mem_pestat] mean and std.dev: (768.42, 472.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2939)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675150 reads in 173.562 CPU sec, 5.590 real sec
[M::process] read 678670 sequences (100000038 bp) in 2.142 CPU sec, 2.157 real sec...
[M::mem_process_seqs] Processed 676022 reads in 122.911 CPU sec, 3.931 real sec
sorting block 54, #reads = 1080030 mem = 536877659 peak = 1078013952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285105, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 301, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (326.71, 154.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676924 sequences (100000014 bp) in 2.513 CPU sec, 2.844 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285136, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 307, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (331.96, 157.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1098)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679206 reads in 166.734 CPU sec, 5.354 real sec
sorting block 55, #reads = 1077288 mem = 536880942 peak = 1078116352
[M::process] read 677042 sequences (100000097 bp) in 2.127 CPU sec, 2.318 real sec...
[M::mem_process_seqs] Processed 678670 reads in 112.892 CPU sec, 3.599 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284966, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 322, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (344.50, 159.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 56, #reads = 1082898 mem = 536879407 peak = 1079820288
[M::process] read 676650 sequences (100000201 bp) in 2.646 CPU sec, 3.045 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284607, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 323, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (344.91, 159.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676924 reads in 164.474 CPU sec, 5.315 real sec
[M::mem_process_seqs] Processed 677042 reads in 111.767 CPU sec, 3.571 real sec
[M::process] read 676618 sequences (100000010 bp) in 2.255 CPU sec, 2.290 real sec...
sorting block 57, #reads = 1081973 mem = 536881977 peak = 1079820288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285154, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 328, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (348.23, 160.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676198 sequences (100000151 bp) in 2.460 CPU sec, 2.715 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284685, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 329, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (348.58, 160.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 349, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1156)
[M::mem_pestat] mean and std.dev: (275.75, 111.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1475)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 676650 reads in 161.909 CPU sec, 5.232 real sec
[M::mem_process_seqs] Processed 676618 reads in 111.665 CPU sec, 3.570 real sec
[M::process] read 676150 sequences (100000229 bp) in 2.247 CPU sec, 2.296 real sec...
sorting block 58, #reads = 1082791 mem = 536879970 peak = 1079820288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284669, 2, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (263, 868, 970)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2384)
[M::mem_pestat] mean and std.dev: (690.30, 372.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3091)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 331, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (350.75, 161.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675958 sequences (100000098 bp) in 2.471 CPU sec, 2.826 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284757, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 334, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (354.39, 163.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676198 reads in 166.296 CPU sec, 5.361 real sec
[M::process] read 675916 sequences (100000007 bp) in 2.240 CPU sec, 2.263 real sec...
[M::mem_process_seqs] Processed 676150 reads in 113.175 CPU sec, 3.590 real sec
sorting block 59, #reads = 1081904 mem = 536878072 peak = 1079820288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284956, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 335, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (355.26, 163.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675758 sequences (100000186 bp) in 2.358 CPU sec, 2.826 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 284471, 1, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (248, 259, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (328.92, 196.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 337, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (355.64, 162.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675958 reads in 165.643 CPU sec, 5.400 real sec
sorting block 60, #reads = 1082658 mem = 536880585 peak = 1088004096
[M::process] read 675586 sequences (100000195 bp) in 2.116 CPU sec, 2.149 real sec...
[M::mem_process_seqs] Processed 675916 reads in 112.128 CPU sec, 3.589 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284525, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 337, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (355.82, 162.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 61, #reads = 1081953 mem = 536879374 peak = 1114923008
[M::process] read 676174 sequences (100000053 bp) in 2.658 CPU sec, 2.969 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284875, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 337, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (355.99, 162.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675758 reads in 164.970 CPU sec, 5.385 real sec
[M::process] read 675888 sequences (100000099 bp) in 2.033 CPU sec, 2.206 real sec...
[M::mem_process_seqs] Processed 675586 reads in 113.297 CPU sec, 3.712 real sec
sorting block 62, #reads = 1082238 mem = 536880689 peak = 1114923008
[M::process] read 675098 sequences (100000063 bp) in 2.455 CPU sec, 2.713 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285053, 9, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 334, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (353.44, 161.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675888 reads in 96.233 CPU sec, 3.124 real sec
[M::process] read 675154 sequences (100000010 bp) in 2.100 CPU sec, 2.133 real sec...
sorting block 63, #reads = 1080209 mem = 536882027 peak = 1114923008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285202, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 331, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (351.59, 161.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284026, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 340, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (358.22, 161.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676174 reads in 259.290 CPU sec, 8.440 real sec
[M::mem_process_seqs] Processed 675098 reads in 132.333 CPU sec, 4.306 real sec
[M::process] read 675118 sequences (100000125 bp) in 2.526 CPU sec, 2.751 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284682, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 336, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (355.09, 160.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 64, #reads = 1082025 mem = 536876135 peak = 1114923008
[M::process] read 674852 sequences (100000272 bp) in 2.752 CPU sec, 3.148 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284651, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 335, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (352.52, 157.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675154 reads in 167.762 CPU sec, 5.416 real sec
[M::mem_process_seqs] Processed 675118 reads in 114.229 CPU sec, 3.682 real sec
[M::process] read 675168 sequences (100000018 bp) in 2.337 CPU sec, 2.417 real sec...
sorting block 65, #reads = 1080475 mem = 536880807 peak = 1114923008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284283, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 333, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (351.40, 156.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675318 sequences (100000107 bp) in 2.668 CPU sec, 2.988 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284985, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 329, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (348.26, 155.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674852 reads in 166.739 CPU sec, 5.393 real sec
[M::mem_process_seqs] Processed 675168 reads in 114.082 CPU sec, 3.650 real sec
[M::process] read 675370 sequences (100000041 bp) in 2.314 CPU sec, 2.371 real sec...
sorting block 66, #reads = 1079453 mem = 536877973 peak = 1114923008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284699, 8, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 329, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (347.43, 155.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (277, 312, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (207, 382)
[M::mem_pestat] mean and std.dev: (296.23, 17.08)
[M::mem_pestat] low and high boundaries for proper pairs: (172, 417)
[M::mem_pestat] skip orientation RR
[M::process] read 675424 sequences (100000194 bp) in 2.713 CPU sec, 2.996 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285166, 3, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 330, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (348.68, 156.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 371, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (317.18, 74.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 752)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675318 reads in 170.060 CPU sec, 5.490 real sec
sorting block 67, #reads = 1079504 mem = 536885387 peak = 1114923008
[M::mem_process_seqs] Processed 675370 reads in 115.500 CPU sec, 3.699 real sec
[M::process] read 675226 sequences (100000278 bp) in 2.220 CPU sec, 2.297 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284808, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 330, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (349.36, 157.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 68, #reads = 1080376 mem = 536878890 peak = 1114923008
[M::process] read 675454 sequences (100000126 bp) in 2.594 CPU sec, 2.735 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284246, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 330, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (349.15, 156.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675424 reads in 168.832 CPU sec, 5.413 real sec
[M::process] read 675246 sequences (100000233 bp) in 2.226 CPU sec, 2.270 real sec...
[M::mem_process_seqs] Processed 675226 reads in 115.452 CPU sec, 3.658 real sec
sorting block 69, #reads = 1080061 mem = 536882359 peak = 1114923008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284992, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 331, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (349.68, 157.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675156 sequences (100000092 bp) in 2.678 CPU sec, 2.894 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284440, 9, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 350, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.00, 105.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 333, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (351.00, 157.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675454 reads in 168.200 CPU sec, 5.405 real sec
[M::process] read 674886 sequences (100000130 bp) in 2.131 CPU sec, 2.211 real sec...
[M::mem_process_seqs] Processed 675246 reads in 114.219 CPU sec, 3.637 real sec
sorting block 70, #reads = 1079430 mem = 536879337 peak = 1114923008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284337, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (418, 418, 589)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (76, 931)
[M::mem_pestat] mean and std.dev: (450.36, 95.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 334, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (352.62, 158.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674810 sequences (100000211 bp) in 2.616 CPU sec, 3.064 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284583, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 337, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (354.98, 159.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675156 reads in 163.710 CPU sec, 5.299 real sec
[M::mem_process_seqs] Processed 674886 reads in 111.586 CPU sec, 3.559 real sec
[M::process] read 674982 sequences (100000138 bp) in 2.363 CPU sec, 2.427 real sec...
sorting block 71, #reads = 1079114 mem = 536879494 peak = 1114923008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285078, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 339, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (357.67, 161.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677132 sequences (100000138 bp) in 2.679 CPU sec, 3.109 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284695, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 338, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (356.87, 161.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674810 reads in 166.324 CPU sec, 5.361 real sec
sorting block 72, #reads = 1079108 mem = 536879356 peak = 1114923008
[M::mem_process_seqs] Processed 674982 reads in 115.612 CPU sec, 3.683 real sec
[M::process] read 680828 sequences (100000045 bp) in 2.584 CPU sec, 2.633 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 285468, 5, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (597, 873, 1120)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2166)
[M::mem_pestat] mean and std.dev: (782.07, 351.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2689)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 322, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (343.85, 159.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 73, #reads = 1079016 mem = 536878943 peak = 1114923008
[M::process] read 679744 sequences (100000274 bp) in 2.487 CPU sec, 2.908 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286654, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 291, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (319.43, 152.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677132 reads in 186.339 CPU sec, 6.034 real sec
[M::process] read 679546 sequences (100000120 bp) in 2.325 CPU sec, 2.385 real sec...
[M::mem_process_seqs] Processed 680828 reads in 128.910 CPU sec, 4.123 real sec
sorting block 74, #reads = 1078522 mem = 536877482 peak = 1114923008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285945, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 302, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881)
[M::mem_pestat] mean and std.dev: (328.68, 155.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1106)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679372 sequences (100000005 bp) in 2.647 CPU sec, 2.949 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 286348, 4, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (267, 717, 816)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1914)
[M::mem_pestat] mean and std.dev: (554.43, 289.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2463)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 303, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (327.72, 154.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 678496 sequences (100000147 bp) in 2.124 CPU sec, 2.125 real sec...
[M::mem_process_seqs] Processed 679744 reads in 185.229 CPU sec, 5.961 real sec
[M::mem_process_seqs] Processed 679546 reads in 123.010 CPU sec, 3.951 real sec
sorting block 75, #reads = 1079081 mem = 536878477 peak = 1114923008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286434, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 307, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (330.05, 153.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677936 sequences (100000173 bp) in 2.692 CPU sec, 3.122 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285816, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 312, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (333.67, 153.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679372 reads in 178.291 CPU sec, 5.743 real sec
[M::process] read 678086 sequences (100000036 bp) in 2.167 CPU sec, 2.181 real sec...
[M::mem_process_seqs] Processed 678496 reads in 120.027 CPU sec, 3.834 real sec
sorting block 76, #reads = 1079834 mem = 536879031 peak = 1114923008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285552, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 317, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (338.35, 156.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677776 sequences (100000125 bp) in 2.727 CPU sec, 3.068 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285986, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 317, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (338.40, 155.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677936 reads in 180.057 CPU sec, 5.791 real sec
sorting block 77, #reads = 1080066 mem = 536879333 peak = 1114923008
[M::process] read 677446 sequences (100000168 bp) in 2.226 CPU sec, 2.232 real sec...
[M::mem_process_seqs] Processed 678086 reads in 120.850 CPU sec, 3.865 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285779, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 322, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (342.00, 157.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 78, #reads = 1080085 mem = 536879104 peak = 1114923008
[M::process] read 677488 sequences (100000159 bp) in 2.761 CPU sec, 2.971 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285214, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 322, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (341.85, 157.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677776 reads in 180.126 CPU sec, 5.852 real sec
[M::process] read 677420 sequences (100000034 bp) in 2.204 CPU sec, 2.227 real sec...
[M::mem_process_seqs] Processed 677446 reads in 121.312 CPU sec, 3.952 real sec
sorting block 79, #reads = 1079996 mem = 536879171 peak = 1114923008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285332, 9, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 322, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (341.64, 156.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677360 sequences (100000131 bp) in 2.562 CPU sec, 2.817 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285740, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 323, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (342.91, 157.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677488 reads in 177.586 CPU sec, 5.729 real sec
[M::process] read 677290 sequences (100000013 bp) in 2.201 CPU sec, 2.223 real sec...
[M::mem_process_seqs] Processed 677420 reads in 118.855 CPU sec, 3.806 real sec
sorting block 80, #reads = 1079744 mem = 536881609 peak = 1114923008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285410, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 322, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (342.47, 156.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677344 sequences (100000233 bp) in 2.749 CPU sec, 3.172 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285244, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 324, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (343.48, 157.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677360 reads in 181.870 CPU sec, 5.877 real sec
[M::process] read 677220 sequences (100000195 bp) in 2.251 CPU sec, 2.273 real sec...
sorting block 81, #reads = 1079725 mem = 536880713 peak = 1114923008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285087, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 326, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (344.12, 157.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677344 reads in 105.811 CPU sec, 3.537 real sec
[M::process] read 677196 sequences (100000073 bp) in 2.330 CPU sec, 2.346 real sec...
[M::mem_process_seqs] Processed 677290 reads in 265.709 CPU sec, 8.669 real sec
sorting block 82, #reads = 1078633 mem = 536880738 peak = 1114923008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285640, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 325, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (343.96, 157.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677008 sequences (100000211 bp) in 2.827 CPU sec, 3.098 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285093, 8, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 326, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (345.14, 158.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (405, 655, 2862)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7776)
[M::mem_pestat] mean and std.dev: (1227.27, 1289.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10233)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 677220 reads in 205.280 CPU sec, 6.558 real sec
[M::mem_process_seqs] Processed 677196 reads in 124.344 CPU sec, 3.938 real sec
[M::process] read 676632 sequences (100000067 bp) in 2.405 CPU sec, 2.481 real sec...
sorting block 83, #reads = 1078681 mem = 536877590 peak = 1114923008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285211, 3, 23)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 327, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (346.26, 157.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (296, 354, 3806)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10826)
[M::mem_pestat] mean and std.dev: (1498.70, 1687.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14336)
[M::mem_pestat] skip orientation RR
[M::process] read 676478 sequences (100000195 bp) in 2.830 CPU sec, 3.159 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285165, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 328, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (346.88, 157.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677008 reads in 186.879 CPU sec, 6.033 real sec
[M::process] read 676478 sequences (100000207 bp) in 2.226 CPU sec, 2.296 real sec...
sorting block 84, #reads = 1078894 mem = 536879969 peak = 1114923008
[M::mem_process_seqs] Processed 676632 reads in 126.699 CPU sec, 4.079 real sec
sorting block 85, #reads = 1077416 mem = 536882182 peak = 1114923008
[M::process] read 676246 sequences (100000166 bp) in 2.823 CPU sec, 3.188 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285020, 2, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 327, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (344.70, 154.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (352, 401, 2464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6688)
[M::mem_pestat] mean and std.dev: (1225.00, 1336.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8800)
[M::mem_pestat] skip orientation RR
[M::process] read 676080 sequences (100000204 bp) in 2.107 CPU sec, 2.144 real sec...
[M::mem_process_seqs] Processed 676478 reads in 108.284 CPU sec, 3.444 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284654, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 328, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (346.08, 155.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 284410, 1, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (287, 310, 8579)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 25163)
[M::mem_pestat] mean and std.dev: (2493.13, 3670.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 33455)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 326, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (343.43, 152.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 676478 reads in 295.457 CPU sec, 9.601 real sec
[M::process] read 675948 sequences (100000054 bp) in 2.498 CPU sec, 2.556 real sec...
sorting block 86, #reads = 1077701 mem = 536880047 peak = 1114923008
[M::mem_process_seqs] Processed 676246 reads in 149.761 CPU sec, 4.887 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 285054, 5, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (417, 417, 2968)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8070)
[M::mem_pestat] mean and std.dev: (1079.50, 1096.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10621)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 329, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (346.84, 154.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 87, #reads = 1076850 mem = 536879772 peak = 1116971008
[M::process] read 675568 sequences (100000010 bp) in 2.948 CPU sec, 3.200 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285457, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 330, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (347.28, 154.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676080 reads in 186.365 CPU sec, 6.043 real sec
[M::process] read 675896 sequences (100000067 bp) in 2.149 CPU sec, 2.259 real sec...
[M::mem_process_seqs] Processed 675948 reads in 125.683 CPU sec, 4.105 real sec
sorting block 88, #reads = 1076951 mem = 536877676 peak = 1116971008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284975, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 334, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (350.12, 155.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675706 sequences (100000240 bp) in 2.666 CPU sec, 2.886 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285015, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 329, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (348.01, 156.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675998 sequences (100000218 bp) in 2.205 CPU sec, 2.212 real sec...
[M::mem_process_seqs] Processed 675568 reads in 186.973 CPU sec, 6.027 real sec
[M::mem_process_seqs] Processed 675896 reads in 125.472 CPU sec, 4.052 real sec
sorting block 89, #reads = 1077436 mem = 536879069 peak = 1116971008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285270, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 334, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (352.33, 159.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682084 sequences (100000063 bp) in 2.790 CPU sec, 3.208 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285184, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 331, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (350.32, 158.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675706 reads in 184.846 CPU sec, 5.959 real sec
[M::process] read 683250 sequences (100000283 bp) in 2.291 CPU sec, 2.298 real sec...
[M::mem_process_seqs] Processed 675998 reads in 126.908 CPU sec, 4.048 real sec
sorting block 90, #reads = 1077570 mem = 536878616 peak = 1116971008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287109, 3, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (265, 302, 544)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1102)
[M::mem_pestat] mean and std.dev: (324.67, 126.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1381)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 284, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (309.78, 146.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 682714 sequences (100000071 bp) in 2.640 CPU sec, 2.974 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287830, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 276, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (301.30, 140.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681748 sequences (100000239 bp) in 2.337 CPU sec, 2.343 real sec...
[M::mem_process_seqs] Processed 682084 reads in 216.609 CPU sec, 6.964 real sec
sorting block 91, #reads = 1076075 mem = 536877965 peak = 1124691968
[M::mem_process_seqs] Processed 683250 reads in 149.009 CPU sec, 4.763 real sec
sorting block 92, #reads = 1071137 mem = 536878224 peak = 1124691968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287348, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 281, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (306.93, 143.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680892 sequences (100000231 bp) in 2.943 CPU sec, 3.191 real sec...
[M::process] read 680060 sequences (100000160 bp) in 2.327 CPU sec, 2.336 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287222, 4, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (341, 430, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (397.30, 139.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1085)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 289, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (312.94, 145.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 682714 reads in 207.346 CPU sec, 6.670 real sec
[M::mem_process_seqs] Processed 681748 reads in 138.201 CPU sec, 4.439 real sec
sorting block 93, #reads = 1073483 mem = 536878940 peak = 1124691968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287019, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 294, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (316.35, 145.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679622 sequences (100000047 bp) in 2.639 CPU sec, 3.150 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286551, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 300, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (320.46, 145.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679018 sequences (100000134 bp) in 2.305 CPU sec, 2.362 real sec...
[M::mem_process_seqs] Processed 680892 reads in 201.973 CPU sec, 6.538 real sec
[M::mem_process_seqs] Processed 680060 reads in 134.767 CPU sec, 4.356 real sec
sorting block 94, #reads = 1073830 mem = 536879310 peak = 1124691968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 287011, 3, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (480, 1272, 2709)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7167)
[M::mem_pestat] mean and std.dev: (1357.77, 968.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9396)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 302, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861)
[M::mem_pestat] mean and std.dev: (323.83, 148.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 678930 sequences (100000115 bp) in 2.913 CPU sec, 3.220 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286596, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 306, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866)
[M::mem_pestat] mean and std.dev: (326.93, 149.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1085)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678626 sequences (100000059 bp) in 2.326 CPU sec, 2.330 real sec...
[M::mem_process_seqs] Processed 679622 reads in 195.846 CPU sec, 6.300 real sec
[M::mem_process_seqs] Processed 679018 reads in 130.254 CPU sec, 4.159 real sec
sorting block 95, #reads = 1074986 mem = 536880977 peak = 1124691968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286187, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 309, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (327.88, 148.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1086)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678378 sequences (100000112 bp) in 2.609 CPU sec, 3.041 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286206, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 311, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (331.08, 151.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1099)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678278 sequences (100000260 bp) in 2.211 CPU sec, 2.214 real sec...
[M::mem_process_seqs] Processed 678930 reads in 193.026 CPU sec, 6.231 real sec
sorting block 96, #reads = 1075959 mem = 536883784 peak = 1128472576
[M::mem_process_seqs] Processed 678626 reads in 128.935 CPU sec, 4.122 real sec
sorting block 97, #reads = 1075304 mem = 536879009 peak = 1129193472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286083, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 313, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (333.54, 152.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678160 sequences (100000218 bp) in 2.954 CPU sec, 3.173 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286802, 9, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 314, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (333.70, 152.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1105)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677584 sequences (100000139 bp) in 2.297 CPU sec, 2.308 real sec...
[M::mem_process_seqs] Processed 678378 reads in 191.341 CPU sec, 6.129 real sec
[M::mem_process_seqs] Processed 678278 reads in 127.674 CPU sec, 4.078 real sec
sorting block 98, #reads = 1076047 mem = 536880004 peak = 1129193472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286100, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 313, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (333.57, 152.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677448 sequences (100000126 bp) in 2.669 CPU sec, 3.223 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 285974, 4, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (350, 352, 882)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1946)
[M::mem_pestat] mean and std.dev: (416.69, 243.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2478)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 317, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (337.31, 154.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 678160 reads in 185.926 CPU sec, 6.013 real sec
[M::process] read 676980 sequences (100000015 bp) in 2.282 CPU sec, 2.299 real sec...
[M::mem_process_seqs] Processed 677584 reads in 127.007 CPU sec, 4.056 real sec
sorting block 99, #reads = 1077466 mem = 536883338 peak = 1129193472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285978, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 321, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (342.48, 157.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676776 sequences (100000190 bp) in 2.815 CPU sec, 3.095 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285104, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 326, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (347.40, 161.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676562 sequences (100000256 bp) in 2.264 CPU sec, 2.266 real sec...
[M::mem_process_seqs] Processed 677448 reads in 185.051 CPU sec, 5.935 real sec
sorting block 100, #reads = 1076727 mem = 536880008 peak = 1129193472
[M::mem_process_seqs] Processed 676980 reads in 123.907 CPU sec, 3.976 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285225, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 330, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (350.19, 161.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 101, #reads = 1077698 mem = 536878315 peak = 1155829760
[M::process] read 676346 sequences (100000194 bp) in 2.694 CPU sec, 2.931 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285600, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 328, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (348.86, 160.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 238, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (263.10, 87.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 676776 reads in 186.756 CPU sec, 6.053 real sec
[M::process] read 676008 sequences (100000040 bp) in 2.256 CPU sec, 2.300 real sec...
[M::mem_process_seqs] Processed 676562 reads in 126.345 CPU sec, 4.097 real sec
sorting block 102, #reads = 1076767 mem = 536879053 peak = 1155829760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284736, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 328, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (348.84, 160.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676058 sequences (100000264 bp) in 2.844 CPU sec, 3.082 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 284825, 9, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (346, 830, 830)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1798)
[M::mem_pestat] mean and std.dev: (620.07, 296.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2282)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (350.27, 159.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675904 sequences (100000174 bp) in 2.236 CPU sec, 2.243 real sec...
[M::mem_process_seqs] Processed 676346 reads in 186.486 CPU sec, 6.023 real sec
[M::mem_process_seqs] Processed 676008 reads in 126.863 CPU sec, 4.119 real sec
sorting block 103, #reads = 1077506 mem = 536878459 peak = 1155829760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285405, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 332, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (352.75, 162.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676034 sequences (100000111 bp) in 2.587 CPU sec, 3.011 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284731, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 335, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (355.33, 163.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676058 reads in 183.923 CPU sec, 5.928 real sec
[M::process] read 675684 sequences (100000273 bp) in 2.190 CPU sec, 2.210 real sec...
[M::mem_process_seqs] Processed 675904 reads in 124.385 CPU sec, 3.970 real sec
sorting block 104, #reads = 1078336 mem = 536878369 peak = 1155829760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285466, 2, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (232, 400, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778)
[M::mem_pestat] mean and std.dev: (318.50, 92.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 333, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (355.04, 165.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 374, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (273.80, 130.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 676290 sequences (100000069 bp) in 3.082 CPU sec, 3.381 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285095, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 337, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (358.62, 166.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676034 reads in 184.916 CPU sec, 5.940 real sec
[M::process] read 680716 sequences (100000166 bp) in 2.354 CPU sec, 2.371 real sec...
sorting block 105, #reads = 1077756 mem = 536880221 peak = 1155829760
[M::mem_process_seqs] Processed 675684 reads in 127.732 CPU sec, 4.070 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285005, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 333, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (354.36, 165.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 106, #reads = 1075997 mem = 536880837 peak = 1159446528
[M::process] read 679640 sequences (100000089 bp) in 2.594 CPU sec, 2.805 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286336, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 291, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (317.26, 150.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678516 sequences (100000250 bp) in 2.193 CPU sec, 2.194 real sec...
[M::mem_process_seqs] Processed 676290 reads in 204.240 CPU sec, 6.534 real sec
[M::mem_process_seqs] Processed 680716 reads in 128.171 CPU sec, 4.055 real sec
sorting block 107, #reads = 1069591 mem = 536879285 peak = 1159446528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286110, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 302, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (326.95, 153.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1091)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678222 sequences (100000102 bp) in 2.736 CPU sec, 2.794 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285986, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 308, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879)
[M::mem_pestat] mean and std.dev: (331.35, 154.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679640 reads in 160.579 CPU sec, 5.057 real sec
[M::process] read 678090 sequences (100000079 bp) in 2.235 CPU sec, 2.240 real sec...
[M::mem_process_seqs] Processed 678516 reads in 108.261 CPU sec, 3.421 real sec
sorting block 108, #reads = 1079059 mem = 536877502 peak = 1159446528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285538, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 312, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (333.79, 154.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1105)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677604 sequences (100000199 bp) in 2.720 CPU sec, 2.887 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285758, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 314, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (335.99, 155.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 239, 1008)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2546)
[M::mem_pestat] mean and std.dev: (575.60, 618.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3315)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 678222 reads in 169.511 CPU sec, 5.420 real sec
[M::mem_process_seqs] Processed 678090 reads in 111.680 CPU sec, 3.515 real sec
[M::process] read 677538 sequences (100000275 bp) in 2.458 CPU sec, 2.516 real sec...
sorting block 109, #reads = 1080572 mem = 536879159 peak = 1159446528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286090, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 316, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (336.92, 154.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1111)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677482 sequences (100000163 bp) in 2.267 CPU sec, 2.358 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 285984, 5, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (370, 374, 2956)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8128)
[M::mem_pestat] mean and std.dev: (1112.31, 1232.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10714)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 317, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (337.04, 153.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 676940 sequences (100000209 bp) in 1.860 CPU sec, 1.854 real sec...
[M::mem_process_seqs] Processed 677604 reads in 164.613 CPU sec, 5.227 real sec
sorting block 110, #reads = 1079859 mem = 536881440 peak = 1159446528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285655, 3, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 318, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (338.38, 155.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 651, 891)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2367)
[M::mem_pestat] mean and std.dev: (547.08, 369.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3105)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 677482 reads in 96.218 CPU sec, 3.059 real sec
[M::process] read 677156 sequences (100000016 bp) in 2.270 CPU sec, 2.290 real sec...
sorting block 111, #reads = 1080244 mem = 536882838 peak = 1159446528
[M::mem_process_seqs] Processed 677538 reads in 243.865 CPU sec, 7.764 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285324, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 322, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (341.66, 156.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676692 sequences (100000295 bp) in 2.596 CPU sec, 2.736 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285524, 0, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (440, 468, 1824)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4592)
[M::mem_pestat] mean and std.dev: (1124.36, 960.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5976)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 322, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (342.41, 156.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 676940 reads in 188.265 CPU sec, 5.998 real sec
[M::mem_process_seqs] Processed 677156 reads in 114.083 CPU sec, 3.599 real sec
sorting block 112, #reads = 1080901 mem = 536885362 peak = 1159446528
[M::process] read 677344 sequences (100000179 bp) in 2.641 CPU sec, 2.770 real sec...
sorting block 113, #reads = 1080617 mem = 536878547 peak = 1159446528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285414, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 317, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (338.76, 155.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677516 sequences (100000154 bp) in 3.360 CPU sec, 3.634 real sec...
[M::mem_process_seqs] Processed 677344 reads in 93.810 CPU sec, 3.025 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285862, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 325, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (345.00, 157.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285913, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 316, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (337.74, 155.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677138 sequences (100000291 bp) in 3.004 CPU sec, 3.171 real sec...
[M::mem_process_seqs] Processed 676692 reads in 265.061 CPU sec, 8.486 real sec
[M::mem_process_seqs] Processed 677516 reads in 118.261 CPU sec, 3.746 real sec
sorting block 114, #reads = 1080496 mem = 536878659 peak = 1159446528
[M::process] read 677166 sequences (100000039 bp) in 2.710 CPU sec, 2.825 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285802, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 322, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (343.14, 157.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676336 sequences (100000219 bp) in 2.589 CPU sec, 2.817 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285860, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 325, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (345.77, 159.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677138 reads in 179.601 CPU sec, 5.760 real sec
sorting block 115, #reads = 1080194 mem = 536878627 peak = 1159446528
[M::mem_process_seqs] Processed 677166 reads in 122.033 CPU sec, 3.893 real sec
[M::process] read 675884 sequences (100000255 bp) in 2.443 CPU sec, 2.515 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285051, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 327, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (347.42, 159.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (393, 393, 8223)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 23883)
[M::mem_pestat] mean and std.dev: (3950.64, 3900.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 31713)
[M::mem_pestat] skip orientation RR
sorting block 116, #reads = 1078254 mem = 536877470 peak = 1159446528
[M::process] read 676050 sequences (100000221 bp) in 2.503 CPU sec, 2.745 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284815, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 332, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (352.23, 161.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676336 reads in 176.003 CPU sec, 5.659 real sec
[M::process] read 675990 sequences (100000277 bp) in 2.057 CPU sec, 2.084 real sec...
[M::mem_process_seqs] Processed 675884 reads in 119.084 CPU sec, 3.761 real sec
sorting block 117, #reads = 1079128 mem = 536880165 peak = 1159446528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284789, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 331, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (351.17, 161.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676006 sequences (100000199 bp) in 2.340 CPU sec, 2.449 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284810, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (350.72, 161.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676532 sequences (100000181 bp) in 1.860 CPU sec, 1.861 real sec...
[M::mem_process_seqs] Processed 676050 reads in 166.389 CPU sec, 5.298 real sec
[M::mem_process_seqs] Processed 675990 reads in 110.109 CPU sec, 3.479 real sec
sorting block 118, #reads = 1078395 mem = 536877388 peak = 1159446528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285157, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 331, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (351.97, 162.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676034 sequences (100000245 bp) in 2.554 CPU sec, 2.624 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285305, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 325, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (347.68, 161.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676004 sequences (100000020 bp) in 2.180 CPU sec, 2.178 real sec...
[M::mem_process_seqs] Processed 676006 reads in 168.758 CPU sec, 5.325 real sec
sorting block 119, #reads = 1077989 mem = 536878731 peak = 1159446528
[M::mem_process_seqs] Processed 676532 reads in 112.156 CPU sec, 3.542 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285175, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 330, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (350.49, 160.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675898 sequences (100000194 bp) in 2.656 CPU sec, 2.710 real sec...
[M::process] read 676038 sequences (100000190 bp) in 1.646 CPU sec, 1.650 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285283, 1, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (244, 483, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (389.82, 140.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 331, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (351.99, 161.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 676004 reads in 163.166 CPU sec, 5.150 real sec
sorting block 120, #reads = 1077461 mem = 536878493 peak = 1159446528
[M::mem_process_seqs] Processed 676034 reads in 180.475 CPU sec, 5.718 real sec
sorting block 121, #reads = 1077774 mem = 536884710 peak = 1159446528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285189, 10, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 331, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (351.88, 161.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (309, 2078, 2224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6054)
[M::mem_pestat] mean and std.dev: (1563.80, 885.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7969)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676000 sequences (100000290 bp) in 2.639 CPU sec, 2.678 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285104, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (351.26, 162.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675898 reads in 164.719 CPU sec, 5.190 real sec
[M::process] read 675986 sequences (100000206 bp) in 2.279 CPU sec, 2.286 real sec...
[M::mem_process_seqs] Processed 676038 reads in 111.283 CPU sec, 3.505 real sec
sorting block 122, #reads = 1077735 mem = 536878730 peak = 1159446528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285096, 12, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 330, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (353.07, 164.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2010, 2438, 2438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1154, 3294)
[M::mem_pestat] mean and std.dev: (2352.20, 171.60)
[M::mem_pestat] low and high boundaries for proper pairs: (726, 3722)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 678940 sequences (100000217 bp) in 2.309 CPU sec, 2.368 real sec...
[M::process] read 684504 sequences (100000255 bp) in 1.937 CPU sec, 1.940 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284884, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 332, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (354.71, 165.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676000 reads in 169.654 CPU sec, 5.363 real sec
[M::mem_process_seqs] Processed 675986 reads in 118.288 CPU sec, 3.755 real sec
sorting block 123, #reads = 1075647 mem = 536880781 peak = 1159446528
[M::process] read 681150 sequences (100000228 bp) in 2.562 CPU sec, 2.620 real sec...
[M::process] read 680418 sequences (100000034 bp) in 1.810 CPU sec, 1.809 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287906, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 270, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (297.92, 142.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684504 reads in 106.227 CPU sec, 3.379 real sec
sorting block 124, #reads = 1072100 mem = 536877261 peak = 1159446528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285758, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 308, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (333.06, 158.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 287218, 0, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (199, 199, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 310)
[M::mem_pestat] mean and std.dev: (197.50, 30.12)
[M::mem_pestat] low and high boundaries for proper pairs: (77, 347)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 293, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (317.71, 148.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 679526 sequences (100000013 bp) in 2.205 CPU sec, 2.217 real sec...
[M::mem_process_seqs] Processed 678940 reads in 311.389 CPU sec, 9.877 real sec
[M::mem_process_seqs] Processed 681150 reads in 240.322 CPU sec, 7.646 real sec
sorting block 125, #reads = 1068887 mem = 536878872 peak = 1159446528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286868, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 298, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (320.78, 148.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678692 sequences (100000103 bp) in 3.323 CPU sec, 3.483 real sec...
[M::process] read 678220 sequences (100000250 bp) in 1.740 CPU sec, 1.734 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286672, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 304, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873)
[M::mem_pestat] mean and std.dev: (326.62, 151.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1095)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680418 reads in 194.139 CPU sec, 6.155 real sec
sorting block 126, #reads = 1073652 mem = 536878374 peak = 1159446528
[M::mem_process_seqs] Processed 679526 reads in 128.123 CPU sec, 4.066 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286072, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 309, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (331.12, 152.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1106)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678024 sequences (100000287 bp) in 3.058 CPU sec, 3.177 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286064, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 314, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (334.56, 152.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677464 sequences (100000122 bp) in 2.220 CPU sec, 2.217 real sec...
[M::mem_process_seqs] Processed 678692 reads in 194.365 CPU sec, 6.160 real sec
sorting block 127, #reads = 1074289 mem = 536879473 peak = 1159446528
[M::mem_process_seqs] Processed 678220 reads in 130.336 CPU sec, 4.135 real sec
sorting block 128, #reads = 1074417 mem = 536879054 peak = 1160601600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286106, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 319, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (338.48, 154.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677208 sequences (100000040 bp) in 2.995 CPU sec, 3.097 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286067, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 323, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (341.41, 154.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677574 sequences (100000168 bp) in 2.231 CPU sec, 2.256 real sec...
[M::mem_process_seqs] Processed 678024 reads in 193.816 CPU sec, 6.208 real sec
[M::mem_process_seqs] Processed 677464 reads in 132.804 CPU sec, 4.274 real sec
sorting block 129, #reads = 1074776 mem = 536879375 peak = 1160601600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286088, 6, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 327, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (344.14, 155.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (141, 231, 752)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1974)
[M::mem_pestat] mean and std.dev: (372.86, 278.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2585)
[M::mem_pestat] skip orientation RR
[M::process] read 676438 sequences (100000000 bp) in 2.921 CPU sec, 3.249 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286061, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 320, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (341.72, 157.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677208 reads in 191.630 CPU sec, 6.233 real sec
[M::process] read 676364 sequences (100000063 bp) in 2.351 CPU sec, 2.396 real sec...
[M::mem_process_seqs] Processed 677574 reads in 130.530 CPU sec, 4.286 real sec
sorting block 130, #reads = 1073803 mem = 536884234 peak = 1160601600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285967, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 329, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (347.74, 156.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676268 sequences (100000158 bp) in 2.725 CPU sec, 3.062 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285703, 2, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 333, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (350.76, 159.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (1287, 1807, 2343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4455)
[M::mem_pestat] mean and std.dev: (1752.79, 937.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5511)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 676438 reads in 188.485 CPU sec, 6.191 real sec
[M::process] read 676190 sequences (100000218 bp) in 2.278 CPU sec, 2.299 real sec...
[M::mem_process_seqs] Processed 676364 reads in 128.235 CPU sec, 4.166 real sec
sorting block 131, #reads = 1075574 mem = 536880253 peak = 1160601600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285399, 12, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 332, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (350.08, 158.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1797, 2364, 2364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (663, 3498)
[M::mem_pestat] mean and std.dev: (2199.20, 252.11)
[M::mem_pestat] low and high boundaries for proper pairs: (96, 4065)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676294 sequences (100000191 bp) in 2.905 CPU sec, 3.296 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285560, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (348.71, 157.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676268 reads in 189.672 CPU sec, 6.501 real sec
sorting block 132, #reads = 1075841 mem = 536879078 peak = 1160601600
[M::process] read 676004 sequences (100000173 bp) in 2.354 CPU sec, 2.482 real sec...
[M::mem_process_seqs] Processed 676190 reads in 129.127 CPU sec, 4.637 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285348, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 325, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (345.65, 156.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 133, #reads = 1076240 mem = 536880785 peak = 1165783040
[M::process] read 675936 sequences (100000140 bp) in 2.973 CPU sec, 3.350 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285059, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (349.85, 159.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676294 reads in 187.617 CPU sec, 6.675 real sec
[M::process] read 675766 sequences (100000010 bp) in 2.363 CPU sec, 2.478 real sec...
[M::mem_process_seqs] Processed 676004 reads in 127.108 CPU sec, 4.494 real sec
sorting block 134, #reads = 1076777 mem = 536881865 peak = 1165783040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285317, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 329, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (349.36, 159.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676044 sequences (100000264 bp) in 2.812 CPU sec, 3.362 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284823, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 328, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (347.61, 157.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 648, 648)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1334)
[M::mem_pestat] mean and std.dev: (486.40, 203.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1677)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675936 reads in 187.184 CPU sec, 6.582 real sec
[M::mem_process_seqs] Processed 675766 reads in 128.439 CPU sec, 4.514 real sec
[M::process] read 675676 sequences (100000249 bp) in 2.513 CPU sec, 2.813 real sec...
sorting block 135, #reads = 1077024 mem = 536880956 peak = 1165783040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285291, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 329, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (348.50, 158.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675440 sequences (100000044 bp) in 3.067 CPU sec, 4.000 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284464, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 331, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (351.11, 159.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676044 reads in 186.110 CPU sec, 6.535 real sec
[M::mem_process_seqs] Processed 675676 reads in 125.564 CPU sec, 4.560 real sec
sorting block 136, #reads = 1076797 mem = 536879061 peak = 1165783040
[M::process] read 675660 sequences (100000241 bp) in 2.441 CPU sec, 2.721 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284972, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 333, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (352.91, 160.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 137, #reads = 1078015 mem = 536879958 peak = 1165783040
[M::process] read 675676 sequences (100000278 bp) in 2.682 CPU sec, 3.460 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285241, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 332, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (353.25, 161.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675440 reads in 182.951 CPU sec, 6.419 real sec
[M::mem_process_seqs] Processed 675660 reads in 123.166 CPU sec, 4.438 real sec
[M::process] read 675422 sequences (100000088 bp) in 2.423 CPU sec, 2.855 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285161, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 333, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (353.97, 162.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 138, #reads = 1077808 mem = 536878615 peak = 1165783040
[M::process] read 675370 sequences (100000134 bp) in 2.682 CPU sec, 3.385 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284838, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 335, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (355.88, 163.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675676 reads in 180.905 CPU sec, 6.258 real sec
[M::process] read 413216 sequences (61112899 bp) in 1.458 CPU sec, 1.508 real sec...
[M::mem_process_seqs] Processed 675422 reads in 122.584 CPU sec, 4.199 real sec
sorting block 139, #reads = 1078003 mem = 536878143 peak = 1165783040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285069, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 338, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (359.08, 165.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 174483, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (351.39, 162.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675370 reads in 145.294 CPU sec, 5.022 real sec
[M::mem_process_seqs] Processed 413216 reads in 83.764 CPU sec, 2.797 real sec
sorting block 140, #reads = 1079674 mem = 536879651 peak = 1165783040
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 152011616
overall: 69882195968 mem 23219.964 user 528.056 sys 783.156 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_d0M-T1-TDNA-1_A23MHWWLT4_1.L005\tPU:L005\tSM:659_d0M-T1-TDNA-1_A23MHWWLT4_1\tLB:659_d0M-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_d0M-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_d0M-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 783.193 sec; CPU: 23748.023 sec
sorting block 141, #reads = 606297 mem = 302198322 peak = 1165783040
uniq header count 1
merging 142 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 8469625405
output reads: 152753867
bam_mem_sort: 142 calls 93.998 user 1.818 sys 99.784 real
bam_write: 142 calls 97.957 user 6.506 sys 213.114 real
execute: 1 calls 58.831 user 5.483 sys 895.722 real
merge_files: 1 calls 58.807 user 5.439 sys 111.795 real
parse_chunk: 2266 calls 137.792 user 32.443 sys 214.441 real
read_chunk: 6104 calls 82.586 user 32.879 sys 772.966 real
sort_block: 1 calls 52.684 user 31.643 sys 719.493 real
write_chunk: 3836 calls 58.167 user 5.065 sys 110.265 real
overall: 3165569024 mem 2948.280 user 109.151 sys 895.744 real
ls: cannot access '*.cram': No such file or directory