.command.log - Workdir - DAQ:SENTIEON_BWAMEM (1136_27F-T1-TDNA-1_A23MHWWLT4_1;L007;0004)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/7c/549bf6fe46967229dadc800683570f/.command.log
Size: 158.4 KB
Task: DAQ:SENTIEON_BWAMEM (1136_27F-T1-TDNA-1_A23MHWWLT4_1;L007;0004)
Attempt
Content

  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/7c/549bf6fe46967229dadc800683570f/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/54/54b4a105dd73c5701d075c209d2dd7/1136_27F-T1-TDNA-1_A23MHWWLT4_1/0004.1136_27F-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/7c/549bf6fe46967229dadc800683570f/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/54/54b4a105dd73c5701d075c209d2dd7/1136_27F-T1-TDNA-1_A23MHWWLT4_1/0004.1136_27F-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz
==> STAGING COMPLETE (7 inputs)

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_27F-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1802.770 CPU sec, 63.707 real sec
[M::process] read 701408 sequences (100000197 bp) in 1.184 CPU sec, 1.189 real sec...
[M::process] read 699508 sequences (100000057 bp) in 1.785 CPU sec, 1.850 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 292385, 19, 59)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 215, 531)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1317)
[M::mem_pestat] mean and std.dev: (314.56, 294.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1710)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 235, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (259.93, 130.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 894)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 285, 1046)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2752)
[M::mem_pestat] mean and std.dev: (549.16, 597.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3605)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 347, 669)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1467)
[M::mem_pestat] mean and std.dev: (426.51, 296.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1866)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 698354 sequences (100000154 bp) in 1.744 CPU sec, 1.780 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 291563, 23, 50)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 330, 1437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3937)
[M::mem_pestat] mean and std.dev: (627.96, 690.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5187)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 239, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (264.61, 132.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (394, 1315, 1961)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5095)
[M::mem_pestat] mean and std.dev: (1197.67, 1088.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6662)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 364, 588)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1348)
[M::mem_pestat] mean and std.dev: (359.14, 237.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1728)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696964 sequences (100000228 bp) in 1.959 CPU sec, 1.980 real sec...
[M::mem_process_seqs] Processed 701408 reads in 184.823 CPU sec, 5.872 real sec
[M::mem_process_seqs] Processed 699508 reads in 113.857 CPU sec, 3.620 real sec
sorting block 0, #reads = 1090886 mem = 536879021 peak = 603041792
[M::process] read 696726 sequences (100000125 bp) in 2.251 CPU sec, 2.303 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 291056, 33, 55)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (226, 338, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (343.07, 202.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 247, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (273.13, 136.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (285, 767, 1990)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5400)
[M::mem_pestat] mean and std.dev: (1230.84, 1267.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7105)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 350, 938)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2384)
[M::mem_pestat] mean and std.dev: (550.75, 459.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3107)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696728 sequences (100000128 bp) in 1.990 CPU sec, 1.993 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 291713, 26, 60)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 263, 770)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1940)
[M::mem_pestat] mean and std.dev: (467.59, 410.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2525)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 244, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (268.48, 133.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (95, 1063, 1701)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4913)
[M::mem_pestat] mean and std.dev: (756.35, 780.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6519)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 398, 674)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1602)
[M::mem_pestat] mean and std.dev: (404.29, 284.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2066)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696964 reads in 168.351 CPU sec, 5.332 real sec
[M::mem_process_seqs] Processed 698354 reads in 189.643 CPU sec, 6.030 real sec
sorting block 1, #reads = 1088211 mem = 536879187 peak = 733589504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 290500, 31, 59)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 323, 683)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1733)
[M::mem_pestat] mean and std.dev: (396.86, 307.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2258)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 248, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (273.35, 136.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (343, 568, 2688)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7378)
[M::mem_pestat] mean and std.dev: (1893.63, 2541.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12059)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 310, 717)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1711)
[M::mem_pestat] mean and std.dev: (370.90, 277.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2208)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695824 sequences (100000136 bp) in 2.544 CPU sec, 2.587 real sec...
[M::process] read 695172 sequences (100000274 bp) in 1.924 CPU sec, 1.949 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 290641, 29, 73)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 307, 543)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1279)
[M::mem_pestat] mean and std.dev: (356.34, 288.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1647)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 248, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (272.81, 136.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (205, 241, 1166)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3088)
[M::mem_pestat] mean and std.dev: (504.65, 584.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4049)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 258, 622)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1486)
[M::mem_pestat] mean and std.dev: (325.77, 248.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1918)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696726 reads in 169.405 CPU sec, 5.358 real sec
[M::mem_process_seqs] Processed 696728 reads in 116.074 CPU sec, 3.690 real sec
sorting block 2, #reads = 1089249 mem = 536881890 peak = 861872128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (95, 290288, 38, 64)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (259, 362, 880)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2122)
[M::mem_pestat] mean and std.dev: (506.95, 463.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2743)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 251, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (274.96, 136.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (227, 567, 2902)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8252)
[M::mem_pestat] mean and std.dev: (1446.84, 1780.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10927)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 218, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052)
[M::mem_pestat] mean and std.dev: (293.14, 223.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1348)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695096 sequences (100000123 bp) in 2.787 CPU sec, 2.882 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 289777, 35, 58)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 273, 841)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2193)
[M::mem_pestat] mean and std.dev: (472.32, 512.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2869)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 251, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (274.41, 134.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (380, 778, 1603)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4049)
[M::mem_pestat] mean and std.dev: (722.86, 530.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5272)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 238, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1123)
[M::mem_pestat] mean and std.dev: (292.92, 187.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1434)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695758 sequences (100000288 bp) in 2.141 CPU sec, 2.143 real sec...
[M::mem_process_seqs] Processed 695824 reads in 170.544 CPU sec, 5.401 real sec
sorting block 3, #reads = 1087537 mem = 536878903 peak = 867266560
[M::mem_process_seqs] Processed 695172 reads in 115.393 CPU sec, 3.657 real sec
sorting block 4, #reads = 1086986 mem = 536879796 peak = 867266560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 289853, 27, 70)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 255, 692)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1702)
[M::mem_pestat] mean and std.dev: (441.65, 409.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2207)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 251, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (274.53, 134.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (106, 461, 958)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2662)
[M::mem_pestat] mean and std.dev: (415.43, 435.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3514)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 334, 582)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1334)
[M::mem_pestat] mean and std.dev: (424.42, 302.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1710)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695260 sequences (100000121 bp) in 2.477 CPU sec, 2.572 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 290036, 29, 87)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 321, 818)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2088)
[M::mem_pestat] mean and std.dev: (474.23, 423.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2723)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 249, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (272.81, 134.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (625, 1216, 2972)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7666)
[M::mem_pestat] mean and std.dev: (1727.90, 1688.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10013)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 329, 624)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1468)
[M::mem_pestat] mean and std.dev: (337.11, 195.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1890)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695422 sequences (100000210 bp) in 1.749 CPU sec, 1.750 real sec...
[M::mem_process_seqs] Processed 695096 reads in 174.858 CPU sec, 5.574 real sec
[M::mem_process_seqs] Processed 695758 reads in 119.761 CPU sec, 3.812 real sec
sorting block 5, #reads = 1085590 mem = 536879195 peak = 867266560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 289948, 19, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 277, 557)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1315)
[M::mem_pestat] mean and std.dev: (335.38, 221.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1694)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 251, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (274.12, 135.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (340, 389, 1080)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2560)
[M::mem_pestat] mean and std.dev: (460.31, 388.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3300)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 268, 610)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1412)
[M::mem_pestat] mean and std.dev: (375.98, 275.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1813)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 700500 sequences (100000260 bp) in 2.792 CPU sec, 2.877 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 290622, 19, 68)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 326, 676)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1688)
[M::mem_pestat] mean and std.dev: (376.20, 301.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2194)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 252, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (276.33, 137.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (361, 453, 842)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1804)
[M::mem_pestat] mean and std.dev: (510.41, 351.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2285)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 267, 596)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1392)
[M::mem_pestat] mean and std.dev: (364.37, 263.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1790)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 698994 sequences (100000236 bp) in 2.197 CPU sec, 2.205 real sec...
[M::mem_process_seqs] Processed 695260 reads in 175.040 CPU sec, 5.548 real sec
[M::mem_process_seqs] Processed 695422 reads in 119.376 CPU sec, 3.778 real sec
sorting block 6, #reads = 1085348 mem = 536880688 peak = 867266560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 292285, 20, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 243, 585)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1437)
[M::mem_pestat] mean and std.dev: (310.95, 248.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1863)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 237, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (261.07, 130.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (352, 920, 2448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6640)
[M::mem_pestat] mean and std.dev: (933.59, 842.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8736)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 241, 619)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1507)
[M::mem_pestat] mean and std.dev: (313.67, 215.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1951)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 697814 sequences (100000144 bp) in 2.515 CPU sec, 2.577 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 291669, 33, 56)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 288, 716)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1846)
[M::mem_pestat] mean and std.dev: (385.08, 354.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2411)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 242, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (265.39, 131.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 910)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (303, 501, 1333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3393)
[M::mem_pestat] mean and std.dev: (655.32, 488.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4423)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 295, 873)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2201)
[M::mem_pestat] mean and std.dev: (479.60, 453.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2865)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700500 reads in 167.651 CPU sec, 5.318 real sec
[M::process] read 697782 sequences (100000106 bp) in 2.139 CPU sec, 2.141 real sec...
sorting block 7, #reads = 1087604 mem = 536879176 peak = 867266560
[M::mem_process_seqs] Processed 698994 reads in 115.353 CPU sec, 3.633 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 291033, 22, 83)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 224, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1089)
[M::mem_pestat] mean and std.dev: (302.88, 235.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1393)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 8, #reads = 1092479 mem = 536879827 peak = 867266560
[M::mem_pestat] (25, 50, 75) percentile: (163, 245, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (268.35, 132.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 513, 663)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1671)
[M::mem_pestat] mean and std.dev: (433.16, 347.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2175)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 309, 586)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1330)
[M::mem_pestat] mean and std.dev: (378.08, 264.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1702)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696622 sequences (100000201 bp) in 2.701 CPU sec, 2.777 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 290950, 34, 70)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 341, 613)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1459)
[M::mem_pestat] mean and std.dev: (421.37, 327.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1882)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 247, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (270.55, 134.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (275, 903, 1430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3740)
[M::mem_pestat] mean and std.dev: (809.58, 576.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4895)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 349, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862)
[M::mem_pestat] mean and std.dev: (344.16, 160.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697814 reads in 168.418 CPU sec, 5.338 real sec
[M::process] read 696432 sequences (100000255 bp) in 2.222 CPU sec, 2.223 real sec...
[M::mem_process_seqs] Processed 697782 reads in 115.263 CPU sec, 3.645 real sec
sorting block 9, #reads = 1089393 mem = 536881558 peak = 867266560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 290968, 28, 58)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 201, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1200)
[M::mem_pestat] mean and std.dev: (289.59, 209.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1545)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 250, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (273.66, 135.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (445, 918, 1242)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2836)
[M::mem_pestat] mean and std.dev: (705.08, 495.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3633)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 339, 632)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1556)
[M::mem_pestat] mean and std.dev: (399.59, 237.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2018)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695756 sequences (100000275 bp) in 2.824 CPU sec, 2.933 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 290519, 31, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 336, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (314.05, 183.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 251, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (275.01, 136.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 368, 951)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2481)
[M::mem_pestat] mean and std.dev: (412.32, 358.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3246)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 295, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (264.33, 101.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696622 reads in 168.826 CPU sec, 5.342 real sec
[M::process] read 695348 sequences (100000035 bp) in 2.249 CPU sec, 2.257 real sec...
[M::mem_process_seqs] Processed 696432 reads in 115.479 CPU sec, 3.638 real sec
sorting block 10, #reads = 1089801 mem = 536883810 peak = 867266560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 290367, 28, 74)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 305, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1217)
[M::mem_pestat] mean and std.dev: (364.50, 263.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1561)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 251, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (274.01, 135.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (52, 429, 730)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2086)
[M::mem_pestat] mean and std.dev: (383.44, 357.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2764)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 270, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (306.83, 190.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695730 sequences (100000047 bp) in 3.062 CPU sec, 3.170 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (89, 289864, 24, 78)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 292, 538)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1238)
[M::mem_pestat] mean and std.dev: (339.46, 200.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1588)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 253, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (275.58, 135.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (227, 599, 949)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2393)
[M::mem_pestat] mean and std.dev: (480.90, 335.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3115)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 299, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (276.62, 137.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695756 reads in 173.698 CPU sec, 5.497 real sec
[M::process] read 695578 sequences (100000174 bp) in 2.345 CPU sec, 2.354 real sec...
sorting block 11, #reads = 1088052 mem = 536879624 peak = 867266560
[M::mem_process_seqs] Processed 695348 reads in 119.815 CPU sec, 3.774 real sec
[M::process] read 695234 sequences (100000057 bp) in 3.033 CPU sec, 3.171 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 290104, 25, 62)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 284, 550)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1318)
[M::mem_pestat] mean and std.dev: (313.82, 211.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1702)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 250, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (273.60, 135.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (368, 764, 1543)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3893)
[M::mem_pestat] mean and std.dev: (1080.24, 895.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5068)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 308, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1143)
[M::mem_pestat] mean and std.dev: (287.34, 171.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1473)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695578 reads in 97.136 CPU sec, 3.051 real sec
sorting block 12, #reads = 1087425 mem = 536878584 peak = 927121408
[M::process] read 695242 sequences (100000044 bp) in 2.450 CPU sec, 2.471 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 290159, 23, 65)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 284, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (295.05, 178.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 249, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (272.99, 134.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 646, 1541)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4313)
[M::mem_pestat] mean and std.dev: (861.33, 984.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5699)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 302, 604)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1420)
[M::mem_pestat] mean and std.dev: (379.07, 262.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1828)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 290275, 26, 66)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 278, 548)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1274)
[M::mem_pestat] mean and std.dev: (318.51, 197.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1637)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 251, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (274.24, 135.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (244, 529, 1707)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4633)
[M::mem_pestat] mean and std.dev: (803.36, 623.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6096)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 306, 623)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1503)
[M::mem_pestat] mean and std.dev: (357.86, 251.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1943)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695730 reads in 269.299 CPU sec, 8.532 real sec
[M::mem_process_seqs] Processed 695234 reads in 139.539 CPU sec, 4.412 real sec
[M::process] read 700210 sequences (100000266 bp) in 2.876 CPU sec, 2.952 real sec...
sorting block 13, #reads = 1086735 mem = 536878924 peak = 927121408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 289827, 17, 83)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 348, 774)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1936)
[M::mem_pestat] mean and std.dev: (432.09, 342.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2517)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 252, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759)
[M::mem_pestat] mean and std.dev: (276.94, 138.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 956)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (311, 479, 1037)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2489)
[M::mem_pestat] mean and std.dev: (611.75, 448.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3215)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 336, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (306.97, 155.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 698524 sequences (100000093 bp) in 2.550 CPU sec, 2.653 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 291956, 24, 65)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (272, 395, 968)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2360)
[M::mem_pestat] mean and std.dev: (562.58, 434.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3056)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 238, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (262.04, 129.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 1209, 2145)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6049)
[M::mem_pestat] mean and std.dev: (1249.54, 1052.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8001)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 406, 973)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2543)
[M::mem_pestat] mean and std.dev: (523.02, 561.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3328)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695242 reads in 172.506 CPU sec, 5.508 real sec
[M::process] read 698038 sequences (100000232 bp) in 1.972 CPU sec, 1.984 real sec...
sorting block 14, #reads = 1085261 mem = 536878200 peak = 927121408
[M::mem_process_seqs] Processed 700210 reads in 117.535 CPU sec, 3.716 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 291668, 34, 62)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 288, 642)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1570)
[M::mem_pestat] mean and std.dev: (358.58, 312.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2034)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 243, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (265.89, 131.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 914)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (265, 687, 1324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3442)
[M::mem_pestat] mean and std.dev: (797.41, 677.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4501)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 268, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1240)
[M::mem_pestat] mean and std.dev: (339.84, 253.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1596)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 15, #reads = 1088818 mem = 536881124 peak = 927121408
[M::process] read 697404 sequences (100000125 bp) in 3.123 CPU sec, 3.225 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 291106, 22, 62)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 284, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1169)
[M::mem_pestat] mean and std.dev: (344.95, 255.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1504)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 245, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (268.77, 133.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (281, 881, 3059)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8615)
[M::mem_pestat] mean and std.dev: (1695.32, 1775.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11393)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 302, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (299.83, 155.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698524 reads in 168.635 CPU sec, 5.374 real sec
[M::mem_process_seqs] Processed 698038 reads in 117.646 CPU sec, 3.706 real sec
[M::process] read 696452 sequences (100000133 bp) in 2.625 CPU sec, 2.677 real sec...
sorting block 16, #reads = 1090230 mem = 536882778 peak = 927121408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 291406, 23, 70)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 301, 783)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1999)
[M::mem_pestat] mean and std.dev: (474.50, 482.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2607)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 249, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (272.28, 135.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (307, 599, 1688)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4450)
[M::mem_pestat] mean and std.dev: (763.52, 671.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5831)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (275, 364, 698)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1544)
[M::mem_pestat] mean and std.dev: (450.21, 325.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1967)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695728 sequences (100000233 bp) in 2.369 CPU sec, 2.481 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 290577, 29, 76)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (220, 324, 620)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1420)
[M::mem_pestat] mean and std.dev: (387.51, 287.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1820)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 250, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (274.29, 136.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 561, 1358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3722)
[M::mem_pestat] mean and std.dev: (710.25, 632.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4904)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 376, 835)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2081)
[M::mem_pestat] mean and std.dev: (473.46, 371.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2704)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697404 reads in 169.439 CPU sec, 5.399 real sec
[M::process] read 695112 sequences (100000296 bp) in 2.093 CPU sec, 2.102 real sec...
[M::mem_process_seqs] Processed 696452 reads in 117.606 CPU sec, 3.709 real sec
sorting block 17, #reads = 1089067 mem = 536879391 peak = 927121408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 290422, 27, 97)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 346, 699)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1713)
[M::mem_pestat] mean and std.dev: (493.32, 381.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2220)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 253, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (276.28, 136.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (78, 548, 1214)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3486)
[M::mem_pestat] mean and std.dev: (769.22, 804.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4622)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 315, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (307.45, 141.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695302 sequences (100000075 bp) in 3.043 CPU sec, 3.185 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 290137, 40, 84)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 318, 608)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1450)
[M::mem_pestat] mean and std.dev: (393.54, 305.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1871)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 253, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (276.27, 136.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 880, 1380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3848)
[M::mem_pestat] mean and std.dev: (971.54, 818.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5082)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 355, 746)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1854)
[M::mem_pestat] mean and std.dev: (437.31, 335.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2408)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695728 reads in 164.572 CPU sec, 5.229 real sec
[M::process] read 695576 sequences (100000084 bp) in 2.223 CPU sec, 2.248 real sec...
[M::mem_process_seqs] Processed 695112 reads in 114.255 CPU sec, 3.600 real sec
sorting block 18, #reads = 1087586 mem = 536878215 peak = 927121408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 290551, 32, 71)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 296, 759)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1907)
[M::mem_pestat] mean and std.dev: (384.28, 294.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2481)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 251, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (274.00, 134.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (292, 526, 793)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1795)
[M::mem_pestat] mean and std.dev: (578.87, 388.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2296)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 267, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (235.55, 93.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 19, #reads = 1087182 mem = 536877176 peak = 943017984
[M::process] read 695340 sequences (100000010 bp) in 2.732 CPU sec, 2.839 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 290227, 29, 82)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 271, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759)
[M::mem_pestat] mean and std.dev: (271.45, 128.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 249, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (272.57, 133.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 293, 876)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2262)
[M::mem_pestat] mean and std.dev: (474.46, 443.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2955)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 261, 708)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1742)
[M::mem_pestat] mean and std.dev: (403.81, 333.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2259)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695302 reads in 171.412 CPU sec, 5.443 real sec
[M::process] read 695556 sequences (100000278 bp) in 2.291 CPU sec, 2.303 real sec...
[M::mem_process_seqs] Processed 695576 reads in 118.367 CPU sec, 3.732 real sec
sorting block 20, #reads = 1088036 mem = 536878650 peak = 943017984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 289913, 17, 68)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 319, 840)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2220)
[M::mem_pestat] mean and std.dev: (485.03, 484.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2910)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 249, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734)
[M::mem_pestat] mean and std.dev: (271.59, 132.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (250, 367, 735)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1705)
[M::mem_pestat] mean and std.dev: (408.38, 232.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2190)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 249, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1239)
[M::mem_pestat] mean and std.dev: (320.92, 247.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1596)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695156 sequences (100000042 bp) in 3.127 CPU sec, 3.221 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 289973, 26, 69)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 321, 812)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2080)
[M::mem_pestat] mean and std.dev: (487.43, 466.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2714)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 250, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (273.21, 134.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 445, 1414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4198)
[M::mem_pestat] mean and std.dev: (750.13, 1004.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5590)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 278, 721)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1811)
[M::mem_pestat] mean and std.dev: (396.03, 374.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2356)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695340 reads in 170.632 CPU sec, 5.420 real sec
[M::mem_process_seqs] Processed 695556 reads in 118.633 CPU sec, 3.739 real sec
[M::process] read 698872 sequences (100000043 bp) in 2.573 CPU sec, 2.613 real sec...
sorting block 21, #reads = 1086343 mem = 536879202 peak = 943017984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 290311, 39, 84)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 267, 605)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1477)
[M::mem_pestat] mean and std.dev: (304.18, 216.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1913)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 251, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (276.42, 137.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (350, 465, 1027)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2381)
[M::mem_pestat] mean and std.dev: (691.64, 528.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3058)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 276, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864)
[M::mem_pestat] mean and std.dev: (286.22, 171.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1100)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 699026 sequences (100000221 bp) in 2.730 CPU sec, 2.812 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 291421, 24, 69)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (234, 288, 799)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1929)
[M::mem_pestat] mean and std.dev: (489.75, 416.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2494)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 242, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734)
[M::mem_pestat] mean and std.dev: (267.00, 133.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (429, 874, 1523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3711)
[M::mem_pestat] mean and std.dev: (1086.92, 794.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4805)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 347, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (335.71, 177.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695156 reads in 170.682 CPU sec, 5.427 real sec
[M::process] read 697588 sequences (100000226 bp) in 2.348 CPU sec, 2.370 real sec...
[M::mem_process_seqs] Processed 698872 reads in 118.451 CPU sec, 3.734 real sec
sorting block 22, #reads = 1087099 mem = 536878783 peak = 943017984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 291414, 27, 48)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 276, 713)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1749)
[M::mem_pestat] mean and std.dev: (406.25, 367.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2267)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 241, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734)
[M::mem_pestat] mean and std.dev: (266.61, 133.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (331, 525, 1562)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4024)
[M::mem_pestat] mean and std.dev: (936.62, 1005.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5255)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 374, 805)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2041)
[M::mem_pestat] mean and std.dev: (536.56, 455.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2659)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 697144 sequences (100000243 bp) in 2.756 CPU sec, 2.847 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 290873, 31, 85)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 314, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1249)
[M::mem_pestat] mean and std.dev: (339.58, 214.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1615)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 246, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (270.06, 134.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (631, 976, 2152)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5194)
[M::mem_pestat] mean and std.dev: (1633.53, 1583.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7967)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 281, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (285.65, 162.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699026 reads in 165.565 CPU sec, 5.251 real sec
sorting block 23, #reads = 1089859 mem = 536878706 peak = 943017984
[M::process] read 696268 sequences (100000225 bp) in 2.332 CPU sec, 2.343 real sec...
[M::mem_process_seqs] Processed 697588 reads in 112.779 CPU sec, 3.553 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 291484, 17, 77)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 475, 1279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3491)
[M::mem_pestat] mean and std.dev: (664.74, 802.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4597)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 249, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (273.18, 136.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (220, 484, 1230)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3250)
[M::mem_pestat] mean and std.dev: (521.20, 469.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4260)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 256, 904)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2388)
[M::mem_pestat] mean and std.dev: (468.68, 441.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3130)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 24, #reads = 1090993 mem = 536879902 peak = 983453696
[M::process] read 696080 sequences (100000298 bp) in 3.052 CPU sec, 3.140 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 290364, 40, 51)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 311, 636)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1496)
[M::mem_pestat] mean and std.dev: (372.61, 274.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1926)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 251, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (275.91, 138.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (223, 790, 1771)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4867)
[M::mem_pestat] mean and std.dev: (864.14, 749.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6415)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 308, 651)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1525)
[M::mem_pestat] mean and std.dev: (372.80, 268.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1962)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697144 reads in 165.670 CPU sec, 5.267 real sec
[M::mem_process_seqs] Processed 696268 reads in 115.027 CPU sec, 3.621 real sec
[M::process] read 694786 sequences (100000031 bp) in 2.565 CPU sec, 2.614 real sec...
sorting block 25, #reads = 1090232 mem = 536880939 peak = 983453696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 290375, 24, 71)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 230, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (271.24, 164.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 252, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (276.55, 138.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 963)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (222, 767, 1603)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4365)
[M::mem_pestat] mean and std.dev: (933.14, 1058.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5746)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 289, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1082)
[M::mem_pestat] mean and std.dev: (318.12, 185.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1383)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695756 sequences (100000175 bp) in 2.875 CPU sec, 2.970 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 290067, 25, 63)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 309, 765)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1965)
[M::mem_pestat] mean and std.dev: (500.08, 456.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2565)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 254, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (277.41, 137.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (125, 551, 1444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4082)
[M::mem_pestat] mean and std.dev: (708.04, 863.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5401)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 314, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (334.63, 195.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696080 reads in 167.055 CPU sec, 5.336 real sec
[M::mem_process_seqs] Processed 694786 reads in 115.641 CPU sec, 3.647 real sec
[M::process] read 695962 sequences (100000171 bp) in 2.701 CPU sec, 2.751 real sec...
sorting block 26, #reads = 1089615 mem = 536880952 peak = 983453696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 290236, 30, 63)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 260, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1092)
[M::mem_pestat] mean and std.dev: (283.88, 201.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1400)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 249, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (272.88, 134.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 934)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (630, 1110, 2875)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7365)
[M::mem_pestat] mean and std.dev: (1450.89, 1316.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9610)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 315, 612)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1456)
[M::mem_pestat] mean and std.dev: (339.96, 230.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1878)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695860 sequences (100000067 bp) in 2.898 CPU sec, 2.995 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 290212, 35, 60)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 280, 666)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1692)
[M::mem_pestat] mean and std.dev: (395.50, 313.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2205)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 248, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734)
[M::mem_pestat] mean and std.dev: (271.28, 133.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (256, 382, 1291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3361)
[M::mem_pestat] mean and std.dev: (613.45, 551.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4396)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 234, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (308.00, 205.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695756 reads in 168.664 CPU sec, 5.379 real sec
[M::mem_process_seqs] Processed 695962 reads in 116.376 CPU sec, 3.670 real sec
sorting block 27, #reads = 1086727 mem = 536878505 peak = 983453696
[M::process] read 695382 sequences (100000271 bp) in 2.640 CPU sec, 2.688 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 290117, 22, 66)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 277, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (290.00, 167.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 248, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (271.21, 132.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 926)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (261, 578, 1618)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4332)
[M::mem_pestat] mean and std.dev: (872.70, 953.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5689)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 269, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1114)
[M::mem_pestat] mean and std.dev: (319.12, 243.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1432)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 28, #reads = 1087312 mem = 536886958 peak = 983453696
[M::process] read 694720 sequences (100000114 bp) in 2.830 CPU sec, 2.908 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 289880, 25, 70)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1257)
[M::mem_pestat] mean and std.dev: (275.70, 184.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1622)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 250, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (273.09, 134.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (107, 353, 644)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1718)
[M::mem_pestat] mean and std.dev: (404.92, 334.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2255)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 303, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1159)
[M::mem_pestat] mean and std.dev: (326.60, 186.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1479)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695860 reads in 171.398 CPU sec, 5.459 real sec
[M::mem_process_seqs] Processed 695382 reads in 119.291 CPU sec, 3.760 real sec
[M::process] read 700004 sequences (100000090 bp) in 2.666 CPU sec, 2.699 real sec...
sorting block 29, #reads = 1087396 mem = 536878596 peak = 983453696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 289986, 16, 52)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 266, 540)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1246)
[M::mem_pestat] mean and std.dev: (308.62, 219.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1599)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 253, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (277.12, 137.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 519, 1020)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2694)
[M::mem_pestat] mean and std.dev: (500.93, 377.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3531)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 241, 581)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1409)
[M::mem_pestat] mean and std.dev: (312.07, 227.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1823)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 704620 sequences (100000082 bp) in 3.047 CPU sec, 3.159 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 292098, 17, 80)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 304, 600)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1388)
[M::mem_pestat] mean and std.dev: (357.02, 252.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1782)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 237, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (263.37, 132.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (251, 715, 1399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3695)
[M::mem_pestat] mean and std.dev: (892.44, 928.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4843)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 289, 709)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1759)
[M::mem_pestat] mean and std.dev: (424.86, 394.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2284)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 694720 reads in 174.185 CPU sec, 5.528 real sec
[M::mem_process_seqs] Processed 700004 reads in 123.210 CPU sec, 3.882 real sec
[M::process] read 702432 sequences (100000009 bp) in 2.608 CPU sec, 2.636 real sec...
sorting block 30, #reads = 1086680 mem = 536879526 peak = 983453696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 293969, 35, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (224, 343, 590)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1322)
[M::mem_pestat] mean and std.dev: (374.40, 234.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1688)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 225, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (252.14, 128.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 868)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (314, 752, 1734)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4574)
[M::mem_pestat] mean and std.dev: (879.71, 948.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5994)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 301, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1112)
[M::mem_pestat] mean and std.dev: (299.85, 168.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1426)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 699662 sequences (100000257 bp) in 2.995 CPU sec, 3.088 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 293355, 17, 59)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 296, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (295.80, 221.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 231, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (258.91, 132.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (201, 320, 1310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3528)
[M::mem_pestat] mean and std.dev: (610.06, 511.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4637)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 246, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721)
[M::mem_pestat] mean and std.dev: (239.96, 98.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 704620 reads in 179.062 CPU sec, 5.678 real sec
[M::process] read 699010 sequences (100000249 bp) in 2.368 CPU sec, 2.378 real sec...
sorting block 31, #reads = 1089500 mem = 536878071 peak = 983453696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 292648, 23, 73)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 330, 886)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2306)
[M::mem_pestat] mean and std.dev: (594.63, 624.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3093)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 239, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (267.19, 136.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (278, 891, 4136)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11852)
[M::mem_pestat] mean and std.dev: (1894.09, 2117.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 15710)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 324, 589)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1361)
[M::mem_pestat] mean and std.dev: (376.16, 190.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1747)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 699008 sequences (100000088 bp) in 2.483 CPU sec, 2.494 real sec...
[M::mem_process_seqs] Processed 699662 reads in 97.620 CPU sec, 3.075 real sec
[M::mem_process_seqs] Processed 702432 reads in 243.580 CPU sec, 7.697 real sec
sorting block 32, #reads = 1089050 mem = 536880982 peak = 983453696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 291719, 18, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 294, 791)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2027)
[M::mem_pestat] mean and std.dev: (467.77, 430.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2645)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 242, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (269.98, 138.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (564, 763, 1723)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4041)
[M::mem_pestat] mean and std.dev: (969.24, 748.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5200)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 311, 840)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2156)
[M::mem_pestat] mean and std.dev: (598.74, 576.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2906)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 699128 sequences (100000240 bp) in 3.110 CPU sec, 3.218 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 292126, 26, 67)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 298, 734)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1846)
[M::mem_pestat] mean and std.dev: (465.02, 415.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2402)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 241, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (268.85, 136.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 542, 1372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3704)
[M::mem_pestat] mean and std.dev: (788.26, 895.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4870)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 334, 1010)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2590)
[M::mem_pestat] mean and std.dev: (524.34, 551.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3380)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699010 reads in 198.734 CPU sec, 6.299 real sec
[M::mem_process_seqs] Processed 699008 reads in 123.904 CPU sec, 3.906 real sec
[M::process] read 698000 sequences (100000228 bp) in 2.650 CPU sec, 2.713 real sec...
sorting block 33, #reads = 1089606 mem = 536878666 peak = 983453696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 291889, 28, 61)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 284, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1136)
[M::mem_pestat] mean and std.dev: (322.98, 238.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1458)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 241, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (268.35, 136.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 845, 3074)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8840)
[M::mem_pestat] mean and std.dev: (1527.71, 1911.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11723)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 302, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (318.03, 161.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 697612 sequences (100000068 bp) in 2.742 CPU sec, 2.815 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 291243, 23, 69)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 259, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1242)
[M::mem_pestat] mean and std.dev: (278.77, 157.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1597)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 243, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (270.54, 137.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (422, 488, 1000)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2156)
[M::mem_pestat] mean and std.dev: (650.05, 512.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2734)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 329, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1099)
[M::mem_pestat] mean and std.dev: (332.11, 181.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1404)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699128 reads in 180.102 CPU sec, 5.718 real sec
[M::process] read 696578 sequences (100000190 bp) in 2.313 CPU sec, 2.328 real sec...
sorting block 34, #reads = 1086360 mem = 536881946 peak = 983453696
[M::mem_process_seqs] Processed 698000 reads in 119.769 CPU sec, 3.776 real sec
sorting block 35, #reads = 1086609 mem = 536883676 peak = 1024737280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 290864, 31, 85)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 233, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (230.44, 129.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 246, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (272.55, 137.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (123, 438, 1283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3603)
[M::mem_pestat] mean and std.dev: (538.26, 597.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4763)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 314, 556)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1242)
[M::mem_pestat] mean and std.dev: (364.41, 229.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1585)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695800 sequences (100000103 bp) in 3.216 CPU sec, 3.312 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 290764, 22, 75)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 309, 712)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1808)
[M::mem_pestat] mean and std.dev: (432.24, 394.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2356)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 246, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (273.32, 137.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 380, 1260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3392)
[M::mem_pestat] mean and std.dev: (768.95, 761.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4458)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 312, 600)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1388)
[M::mem_pestat] mean and std.dev: (380.16, 270.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1782)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697612 reads in 180.360 CPU sec, 5.711 real sec
[M::process] read 702684 sequences (100000248 bp) in 2.085 CPU sec, 2.091 real sec...
[M::mem_process_seqs] Processed 696578 reads in 124.163 CPU sec, 3.919 real sec
sorting block 36, #reads = 1085534 mem = 536877390 peak = 1024737280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 290635, 31, 78)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 265, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093)
[M::mem_pestat] mean and std.dev: (302.42, 211.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1402)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 249, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (275.82, 139.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 586, 1333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3663)
[M::mem_pestat] mean and std.dev: (812.42, 790.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4828)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 335, 565)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1345)
[M::mem_pestat] mean and std.dev: (409.46, 324.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1735)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 705830 sequences (100000192 bp) in 2.689 CPU sec, 2.753 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 292856, 17, 80)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (201, 269, 555)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1263)
[M::mem_pestat] mean and std.dev: (334.20, 206.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1617)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 230, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (257.33, 131.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (246, 874, 2264)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6300)
[M::mem_pestat] mean and std.dev: (1096.31, 1038.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8318)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 304, 555)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1225)
[M::mem_pestat] mean and std.dev: (331.26, 187.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1560)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 704234 sequences (100000283 bp) in 2.447 CPU sec, 2.454 real sec...
[M::mem_process_seqs] Processed 695800 reads in 189.404 CPU sec, 6.240 real sec
[M::mem_process_seqs] Processed 702684 reads in 125.011 CPU sec, 3.944 real sec
sorting block 37, #reads = 1084761 mem = 536879806 peak = 1024737280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 294119, 28, 69)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (220, 277, 821)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2023)
[M::mem_pestat] mean and std.dev: (374.08, 328.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2624)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 221, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (247.36, 123.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (240, 665, 1292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3396)
[M::mem_pestat] mean and std.dev: (725.81, 632.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4448)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 345, 880)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2274)
[M::mem_pestat] mean and std.dev: (577.45, 620.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3058)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 702684 sequences (100000195 bp) in 2.864 CPU sec, 2.960 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 294027, 31, 72)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 227, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (234.91, 138.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 225, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684)
[M::mem_pestat] mean and std.dev: (251.21, 125.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 861)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (358, 630, 1498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3778)
[M::mem_pestat] mean and std.dev: (712.72, 540.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4918)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 289, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1173)
[M::mem_pestat] mean and std.dev: (343.21, 203.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1498)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 701124 sequences (100000268 bp) in 2.440 CPU sec, 2.441 real sec...
[M::mem_process_seqs] Processed 705830 reads in 196.771 CPU sec, 6.234 real sec
sorting block 38, #reads = 1085593 mem = 536884098 peak = 1024737280
[M::mem_process_seqs] Processed 704234 reads in 134.520 CPU sec, 4.265 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 293332, 31, 48)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 287, 609)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1447)
[M::mem_pestat] mean and std.dev: (400.65, 292.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1866)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 230, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (255.78, 128.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 446, 1050)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2878)
[M::mem_pestat] mean and std.dev: (505.61, 428.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3792)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 363, 685)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1607)
[M::mem_pestat] mean and std.dev: (443.14, 319.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2068)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 39, #reads = 1085431 mem = 536883655 peak = 1024737280
[M::process] read 699834 sequences (100000284 bp) in 2.993 CPU sec, 3.070 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 292889, 19, 60)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 251, 601)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1471)
[M::mem_pestat] mean and std.dev: (349.40, 300.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1906)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 234, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715)
[M::mem_pestat] mean and std.dev: (260.38, 131.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 901)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (609, 621, 1358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2856)
[M::mem_pestat] mean and std.dev: (761.24, 528.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3605)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 297, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (309.54, 182.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 699800 sequences (100000167 bp) in 2.209 CPU sec, 2.220 real sec...
[M::mem_process_seqs] Processed 702684 reads in 195.003 CPU sec, 6.191 real sec
[M::mem_process_seqs] Processed 701124 reads in 132.085 CPU sec, 4.190 real sec
sorting block 40, #reads = 1086193 mem = 536877985 peak = 1024737280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 292601, 18, 61)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 279, 884)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2268)
[M::mem_pestat] mean and std.dev: (479.03, 493.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2960)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 238, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (264.79, 133.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (325, 792, 2073)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5569)
[M::mem_pestat] mean and std.dev: (1133.35, 1081.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7317)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 315, 547)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1165)
[M::mem_pestat] mean and std.dev: (355.02, 240.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1474)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 699150 sequences (100000146 bp) in 2.958 CPU sec, 3.064 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 292653, 28, 60)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 290, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1263)
[M::mem_pestat] mean and std.dev: (291.69, 228.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1633)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 237, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (263.47, 132.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (217, 828, 1277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3397)
[M::mem_pestat] mean and std.dev: (960.71, 879.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4477)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 359, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1113)
[M::mem_pestat] mean and std.dev: (346.41, 160.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1407)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 697586 sequences (100000176 bp) in 2.226 CPU sec, 2.235 real sec...
[M::mem_process_seqs] Processed 699834 reads in 185.256 CPU sec, 5.876 real sec
[M::mem_process_seqs] Processed 699800 reads in 126.927 CPU sec, 4.010 real sec
sorting block 41, #reads = 1084890 mem = 536877720 peak = 1024737280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 292072, 18, 64)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 251, 577)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1411)
[M::mem_pestat] mean and std.dev: (360.14, 295.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1828)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 239, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (266.82, 135.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (402, 761, 1606)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4014)
[M::mem_pestat] mean and std.dev: (1018.28, 921.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5218)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 259, 536)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1280)
[M::mem_pestat] mean and std.dev: (383.89, 288.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1652)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696946 sequences (100000269 bp) in 3.238 CPU sec, 3.314 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 291118, 26, 72)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 235, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1272)
[M::mem_pestat] mean and std.dev: (341.96, 250.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1642)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 244, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (272.95, 140.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 951)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (275, 531, 1834)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4952)
[M::mem_pestat] mean and std.dev: (730.65, 663.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6511)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 350, 862)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2264)
[M::mem_pestat] mean and std.dev: (515.32, 518.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2965)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699150 reads in 179.559 CPU sec, 5.674 real sec
[M::process] read 697878 sequences (100000048 bp) in 2.584 CPU sec, 2.587 real sec...
sorting block 42, #reads = 1085829 mem = 536878935 peak = 1024737280
[M::mem_process_seqs] Processed 697586 reads in 125.441 CPU sec, 3.953 real sec
[M::process] read 703856 sequences (100000180 bp) in 3.104 CPU sec, 3.222 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 291056, 14, 72)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 285, 609)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1469)
[M::mem_pestat] mean and std.dev: (363.23, 241.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1899)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 244, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (272.19, 139.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (305, 561, 698)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1484)
[M::mem_pestat] mean and std.dev: (441.92, 244.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1877)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 296, 621)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1485)
[M::mem_pestat] mean and std.dev: (361.11, 264.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1917)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 701776 sequences (100000176 bp) in 2.395 CPU sec, 2.399 real sec...
[M::mem_process_seqs] Processed 697878 reads in 116.411 CPU sec, 3.664 real sec
sorting block 43, #reads = 1086055 mem = 536881572 peak = 1039581184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 290624, 23, 64)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 317, 1324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3626)
[M::mem_pestat] mean and std.dev: (700.57, 805.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4777)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 246, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (274.55, 140.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 364, 1076)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2850)
[M::mem_pestat] mean and std.dev: (558.95, 609.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3737)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 318, 733)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1749)
[M::mem_pestat] mean and std.dev: (472.06, 365.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2257)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 293694, 21, 63)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 359, 891)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2289)
[M::mem_pestat] mean and std.dev: (498.98, 451.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2988)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 227, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693)
[M::mem_pestat] mean and std.dev: (253.43, 128.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 873)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (371, 475, 932)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2054)
[M::mem_pestat] mean and std.dev: (548.32, 393.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2615)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 289, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1101)
[M::mem_pestat] mean and std.dev: (309.02, 201.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1402)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696946 reads in 294.979 CPU sec, 9.340 real sec
[M::process] read 700586 sequences (100000160 bp) in 2.550 CPU sec, 2.570 real sec...
[M::mem_process_seqs] Processed 703856 reads in 151.921 CPU sec, 4.817 real sec
sorting block 44, #reads = 1080523 mem = 536878039 peak = 1085771776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 292916, 24, 60)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 363, 685)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1689)
[M::mem_pestat] mean and std.dev: (370.77, 245.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2191)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 233, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (259.03, 130.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 893)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (502, 806, 2427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6277)
[M::mem_pestat] mean and std.dev: (815.05, 616.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8202)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 341, 1003)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2597)
[M::mem_pestat] mean and std.dev: (457.31, 415.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3394)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 699578 sequences (100000188 bp) in 3.186 CPU sec, 3.277 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 292747, 30, 68)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (225, 314, 668)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1554)
[M::mem_pestat] mean and std.dev: (438.94, 321.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1997)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 237, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (263.78, 133.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 918)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (360, 603, 1132)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2676)
[M::mem_pestat] mean and std.dev: (758.63, 584.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3448)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 348, 1091)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2809)
[M::mem_pestat] mean and std.dev: (530.83, 487.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3668)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 701776 reads in 175.255 CPU sec, 5.569 real sec
sorting block 45, #reads = 1087115 mem = 536880339 peak = 1085771776
[M::mem_process_seqs] Processed 700586 reads in 119.648 CPU sec, 3.767 real sec
[M::process] read 699382 sequences (100000053 bp) in 2.641 CPU sec, 2.681 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 292418, 16, 70)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 365, 639)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1547)
[M::mem_pestat] mean and std.dev: (406.47, 301.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2001)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 239, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (266.67, 135.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (367, 503, 8460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 24646)
[M::mem_pestat] mean and std.dev: (2488.44, 3456.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 32739)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 337, 587)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1299)
[M::mem_pestat] mean and std.dev: (344.48, 224.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1655)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 46, #reads = 1090071 mem = 536879499 peak = 1085771776
[M::process] read 698032 sequences (100000083 bp) in 2.806 CPU sec, 2.902 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 292208, 14, 70)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 315, 634)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1544)
[M::mem_pestat] mean and std.dev: (364.69, 265.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1999)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 240, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (266.29, 134.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (44, 1393, 1534)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4514)
[M::mem_pestat] mean and std.dev: (1139.93, 966.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6004)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 265, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866)
[M::mem_pestat] mean and std.dev: (266.47, 135.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1092)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699578 reads in 176.812 CPU sec, 5.625 real sec
[M::process] read 697808 sequences (100000239 bp) in 2.329 CPU sec, 2.351 real sec...
[M::mem_process_seqs] Processed 699382 reads in 121.870 CPU sec, 3.834 real sec
sorting block 47, #reads = 1089985 mem = 536883009 peak = 1085771776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 291874, 29, 57)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 257, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1227)
[M::mem_pestat] mean and std.dev: (260.63, 175.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1586)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 244, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (270.58, 136.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (392, 750, 1185)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2771)
[M::mem_pestat] mean and std.dev: (812.15, 615.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3564)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 367, 813)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2077)
[M::mem_pestat] mean and std.dev: (480.68, 406.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2709)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 697108 sequences (100000220 bp) in 2.924 CPU sec, 3.012 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 290974, 21, 90)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 336, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1171)
[M::mem_pestat] mean and std.dev: (321.91, 193.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1502)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 245, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (272.02, 138.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (341, 640, 1514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3860)
[M::mem_pestat] mean and std.dev: (749.63, 602.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5033)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 355, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (335.03, 178.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698032 reads in 174.857 CPU sec, 5.564 real sec
[M::mem_process_seqs] Processed 697808 reads in 120.428 CPU sec, 3.791 real sec
[M::process] read 696980 sequences (100000205 bp) in 2.499 CPU sec, 2.526 real sec...
sorting block 48, #reads = 1087200 mem = 536879783 peak = 1085771776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 290741, 25, 82)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 379, 753)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1923)
[M::mem_pestat] mean and std.dev: (500.73, 458.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2508)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 246, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (272.62, 138.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (255, 452, 1122)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2856)
[M::mem_pestat] mean and std.dev: (547.65, 463.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3723)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 392, 714)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1712)
[M::mem_pestat] mean and std.dev: (468.91, 374.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2211)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 697252 sequences (100000069 bp) in 2.732 CPU sec, 2.808 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 291516, 16, 60)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 302, 831)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2153)
[M::mem_pestat] mean and std.dev: (450.83, 418.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2814)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 246, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (273.46, 138.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (649, 1184, 2352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5758)
[M::mem_pestat] mean and std.dev: (1482.69, 1435.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7461)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 317, 540)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1228)
[M::mem_pestat] mean and std.dev: (389.55, 257.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1572)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697108 reads in 173.811 CPU sec, 5.510 real sec
[M::process] read 696788 sequences (100000275 bp) in 2.447 CPU sec, 2.454 real sec...
sorting block 49, #reads = 1086862 mem = 536881153 peak = 1085771776
[M::mem_process_seqs] Processed 696980 reads in 120.727 CPU sec, 3.799 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 290662, 28, 63)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 253, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1175)
[M::mem_pestat] mean and std.dev: (285.49, 200.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1518)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 245, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (272.20, 137.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (367, 710, 1993)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5245)
[M::mem_pestat] mean and std.dev: (1236.30, 1263.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6871)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 254, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (289.49, 170.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 50, #reads = 1085262 mem = 536880844 peak = 1085771776
[M::process] read 707334 sequences (100000154 bp) in 3.093 CPU sec, 3.170 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 290830, 25, 81)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 284, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1072)
[M::mem_pestat] mean and std.dev: (334.25, 226.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1371)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 246, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (273.32, 138.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (232, 659, 1528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4120)
[M::mem_pestat] mean and std.dev: (779.52, 741.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5416)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 296, 577)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1325)
[M::mem_pestat] mean and std.dev: (353.46, 236.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1699)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697252 reads in 182.864 CPU sec, 5.793 real sec
[M::process] read 704992 sequences (100000006 bp) in 2.427 CPU sec, 2.429 real sec...
[M::mem_process_seqs] Processed 696788 reads in 129.920 CPU sec, 4.095 real sec
sorting block 51, #reads = 1083731 mem = 536879354 peak = 1085771776
[M::process] read 702780 sequences (100000248 bp) in 2.471 CPU sec, 2.547 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 294491, 21, 60)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 291, 563)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1339)
[M::mem_pestat] mean and std.dev: (348.41, 255.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1727)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 218, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (243.67, 122.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (222, 677, 1204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3168)
[M::mem_pestat] mean and std.dev: (776.71, 669.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4150)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 349, 537)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1281)
[M::mem_pestat] mean and std.dev: (355.41, 265.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1653)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 701920 sequences (100000301 bp) in 1.849 CPU sec, 1.852 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 294092, 30, 69)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 303, 683)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1673)
[M::mem_pestat] mean and std.dev: (444.98, 409.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2168)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 224, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (249.88, 125.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 856)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (333, 767, 1436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3642)
[M::mem_pestat] mean and std.dev: (892.57, 767.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4745)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 256, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (272.98, 163.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 707334 reads in 197.936 CPU sec, 6.297 real sec
[M::mem_process_seqs] Processed 704992 reads in 130.616 CPU sec, 4.172 real sec
sorting block 52, #reads = 1081764 mem = 536886563 peak = 1085771776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 293183, 29, 54)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 265, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1243)
[M::mem_pestat] mean and std.dev: (311.74, 204.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1603)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 230, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (255.65, 128.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 863, 3683)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10747)
[M::mem_pestat] mean and std.dev: (1979.76, 2338.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14279)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 283, 664)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1622)
[M::mem_pestat] mean and std.dev: (414.33, 331.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2101)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 700698 sequences (100000045 bp) in 3.111 CPU sec, 3.179 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 292945, 19, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 244, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (309.76, 222.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 232, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (260.11, 132.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (290, 552, 1931)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5213)
[M::mem_pestat] mean and std.dev: (929.47, 789.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6854)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 316, 746)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1846)
[M::mem_pestat] mean and std.dev: (472.61, 431.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2396)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 698284 sequences (100000189 bp) in 2.390 CPU sec, 2.393 real sec...
[M::mem_process_seqs] Processed 702780 reads in 195.664 CPU sec, 6.183 real sec
sorting block 53, #reads = 1086242 mem = 536876805 peak = 1085771776
[M::mem_process_seqs] Processed 701920 reads in 129.067 CPU sec, 4.087 real sec
sorting block 54, #reads = 1085266 mem = 536877614 peak = 1085771776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 292073, 34, 65)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 306, 694)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1726)
[M::mem_pestat] mean and std.dev: (429.56, 369.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2242)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 235, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (261.48, 131.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 902)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (226, 661, 1562)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4234)
[M::mem_pestat] mean and std.dev: (814.07, 888.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5570)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 352, 634)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1548)
[M::mem_pestat] mean and std.dev: (395.49, 303.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2005)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 698370 sequences (100000150 bp) in 2.602 CPU sec, 2.676 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (98, 291558, 21, 101)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 249, 538)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1298)
[M::mem_pestat] mean and std.dev: (328.49, 258.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1678)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 242, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (268.37, 134.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 930)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 665, 1110)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3014)
[M::mem_pestat] mean and std.dev: (641.30, 537.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3966)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 342, 963)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2495)
[M::mem_pestat] mean and std.dev: (556.59, 539.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3261)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 697720 sequences (100000287 bp) in 2.410 CPU sec, 2.407 real sec...
[M::mem_process_seqs] Processed 700698 reads in 184.728 CPU sec, 5.850 real sec
[M::mem_process_seqs] Processed 698284 reads in 123.959 CPU sec, 3.918 real sec
sorting block 55, #reads = 1084726 mem = 536880503 peak = 1085771776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 291108, 28, 70)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 292, 602)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1498)
[M::mem_pestat] mean and std.dev: (339.04, 263.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1946)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 240, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (266.61, 133.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 914)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (196, 649, 2054)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5770)
[M::mem_pestat] mean and std.dev: (904.42, 1046.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7628)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 249, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (272.69, 136.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 697538 sequences (100000196 bp) in 2.693 CPU sec, 2.765 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 290554, 19, 69)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 251, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (302.62, 182.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 242, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (268.41, 134.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (392, 925, 1947)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5057)
[M::mem_pestat] mean and std.dev: (957.47, 701.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6612)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 253, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (279.85, 149.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696410 sequences (100000263 bp) in 2.189 CPU sec, 2.189 real sec...
[M::mem_process_seqs] Processed 698370 reads in 181.214 CPU sec, 5.746 real sec
[M::mem_process_seqs] Processed 697720 reads in 122.479 CPU sec, 3.877 real sec
sorting block 56, #reads = 1084531 mem = 536887010 peak = 1085771776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 291178, 22, 74)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 258, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1203)
[M::mem_pestat] mean and std.dev: (266.44, 200.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1551)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 243, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (270.41, 136.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (110, 488, 804)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2192)
[M::mem_pestat] mean and std.dev: (549.14, 463.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2886)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 343, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (352.66, 205.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 527286 sequences (75723046 bp) in 2.251 CPU sec, 2.312 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 291015, 28, 71)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 189, 573)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1433)
[M::mem_pestat] mean and std.dev: (328.68, 329.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1863)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 248, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759)
[M::mem_pestat] mean and std.dev: (274.98, 139.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (312, 618, 938)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2190)
[M::mem_pestat] mean and std.dev: (641.65, 490.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2816)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 330, 865)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2199)
[M::mem_pestat] mean and std.dev: (481.15, 445.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2866)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697538 reads in 163.935 CPU sec, 5.269 real sec
[M::mem_process_seqs] Processed 696410 reads in 102.724 CPU sec, 3.317 real sec
sorting block 57, #reads = 1085523 mem = 536880650 peak = 1085771776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 220036, 24, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 247, 659)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1589)
[M::mem_pestat] mean and std.dev: (369.02, 323.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2054)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 247, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (273.78, 138.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 496, 2042)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5874)
[M::mem_pestat] mean and std.dev: (797.32, 985.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7790)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 330, 701)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1581)
[M::mem_pestat] mean and std.dev: (435.10, 286.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2021)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 527286 reads in 63.220 CPU sec, 2.091 real sec
sorting block 58, #reads = 1085055 mem = 536876402 peak = 1088131072
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 64061176
overall: 69681729536 mem 10577.870 user 308.636 sys 360.320 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_27F-T1-TDNA-1_A23MHWWLT4_1.L007\tPU:L007\tSM:1136_27F-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_27F-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0004.1136_27F-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0004.1136_27F-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 360.358 sec; CPU: 10886.511 sec
sorting block 59, #reads = 457189 mem = 226649558 peak = 1088131072
uniq header count 1
merging 60 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3678769449
output reads: 64600188
bam_mem_sort: 60 calls 36.453 user 0.799 sys 37.384 real
bam_write: 60 calls 39.356 user 2.231 sys 49.573 real
execute: 1 calls 20.680 user 0.787 sys 388.980 real
merge_files: 1 calls 20.643 user 0.763 sys 28.325 real
parse_chunk: 951 calls 57.748 user 13.492 sys 74.964 real
read_chunk: 2571 calls 28.336 user 14.770 sys 301.353 real
sort_block: 1 calls 21.131 user 14.394 sys 289.854 real
write_chunk: 1618 calls 20.162 user 0.534 sys 27.253 real
overall: 2829246464 mem 1092.279 user 44.738 sys 389.003 real
ls: cannot access '*.cram': No such file or directory