Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/0d/ae68922768dbb62b10370943354cee/.command.sh Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/2a/f64410b73167fcd2a88d3039d55aa5/1136_3LU-T1-TDNA-1_A23MHWWLT4_1/1136_3LU-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/0d/ae68922768dbb62b10370943354cee/.command.run Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/2a/f64410b73167fcd2a88d3039d55aa5/1136_3LU-T1-TDNA-1_A23MHWWLT4_1/1136_3LU-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz ==> STAGING COMPLETE (7 inputs) This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_3LU-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1600.612 CPU sec, 55.310 real sec [M::process] read 683376 sequences (100000019 bp) in 1.495 CPU sec, 1.498 real sec... [M::process] read 682112 sequences (100000278 bp) in 2.100 CPU sec, 2.105 real sec... [M::process] read 680856 sequences (100000151 bp) in 2.139 CPU sec, 2.142 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 283169, 2, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 256, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (274.88, 111.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (219, 344, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005) [M::mem_pestat] mean and std.dev: (359.57, 172.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1267) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 287, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (283.95, 138.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682112 reads in 107.728 CPU sec, 3.362 real sec [M::process] read 680610 sequences (100000088 bp) in 2.260 CPU sec, 2.267 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 283916, 0, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 263, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (263.81, 136.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 335, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989) [M::mem_pestat] mean and std.dev: (351.88, 170.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1248) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (325, 467, 659) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1327) [M::mem_pestat] mean and std.dev: (462.82, 232.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1661) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680372 sequences (100000133 bp) in 1.586 CPU sec, 1.594 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 283636, 2, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 344, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979) [M::mem_pestat] mean and std.dev: (291.74, 162.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1255) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (226, 354, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024) [M::mem_pestat] mean and std.dev: (367.77, 175.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1290) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 364, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1106) [M::mem_pestat] mean and std.dev: (379.02, 175.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1396) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683376 reads in 291.147 CPU sec, 9.144 real sec sorting block 0, #reads = 1082704 mem = 536879198 peak = 651251712 [M::mem_process_seqs] Processed 680856 reads in 150.574 CPU sec, 4.769 real sec [M::process] read 680072 sequences (100000035 bp) in 2.499 CPU sec, 2.523 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 283285, 11, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 249, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (256.60, 111.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (228, 357, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011) [M::mem_pestat] mean and std.dev: (368.29, 173.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1272) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (577, 2465, 2465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6241) [M::mem_pestat] mean and std.dev: (1503.09, 1095.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8129) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 286, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (242.54, 88.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680264 sequences (100000234 bp) in 2.150 CPU sec, 2.145 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 282977, 3, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 388, 815) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2013) [M::mem_pestat] mean and std.dev: (489.78, 331.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (231, 361, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026) [M::mem_pestat] mean and std.dev: (372.35, 174.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1291) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 275, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (309.20, 106.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680610 reads in 186.595 CPU sec, 5.886 real sec sorting block 1, #reads = 1081660 mem = 536883034 peak = 944795648 [M::mem_process_seqs] Processed 680372 reads in 210.014 CPU sec, 6.648 real sec sorting block 2, #reads = 1080989 mem = 536877532 peak = 991911936 [M::process] read 679570 sequences (100000110 bp) in 2.357 CPU sec, 2.382 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282231, 3, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 320, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (295.52, 122.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (233, 364, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028) [M::mem_pestat] mean and std.dev: (374.25, 174.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1293) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (262, 319, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943) [M::mem_pestat] mean and std.dev: (364.93, 149.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1170) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679012 sequences (100000220 bp) in 2.179 CPU sec, 2.181 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 283171, 1, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (252, 411, 623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1365) [M::mem_pestat] mean and std.dev: (382.24, 199.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1736) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (232, 363, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024) [M::mem_pestat] mean and std.dev: (372.89, 174.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1288) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 322, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1163) [M::mem_pestat] mean and std.dev: (305.21, 139.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1476) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282610, 1, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (246, 295, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (6, 606) [M::mem_pestat] mean and std.dev: (282.32, 82.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (236, 370, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040) [M::mem_pestat] mean and std.dev: (378.94, 176.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1308) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 215, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967) [M::mem_pestat] mean and std.dev: (306.92, 201.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1233) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680072 reads in 270.826 CPU sec, 8.618 real sec [M::mem_process_seqs] Processed 680264 reads in 206.727 CPU sec, 6.615 real sec [M::mem_process_seqs] Processed 679570 reads in 231.150 CPU sec, 7.395 real sec sorting block 3, #reads = 1081267 mem = 536877458 peak = 991911936 [M::process] read 679208 sequences (100000135 bp) in 2.811 CPU sec, 2.862 real sec... sorting block 4, #reads = 1080429 mem = 536878553 peak = 1006825472 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282822, 0, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 220, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (211.00, 74.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (239, 373, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046) [M::mem_pestat] mean and std.dev: (382.01, 177.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1315) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (113, 122, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (161.36, 74.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678684 sequences (100000146 bp) in 2.474 CPU sec, 2.477 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 281762, 5, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (281, 633, 5000) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14438) [M::mem_pestat] mean and std.dev: (1824.25, 2176.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19157) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (238, 372, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045) [M::mem_pestat] mean and std.dev: (380.88, 176.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1314) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 260, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (339.33, 229.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1259) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 679012 reads in 200.166 CPU sec, 6.308 real sec [M::process] read 678416 sequences (100000289 bp) in 2.381 CPU sec, 2.393 real sec... [M::mem_process_seqs] Processed 679208 reads in 133.394 CPU sec, 4.171 real sec sorting block 5, #reads = 1079899 mem = 536879608 peak = 1006825472 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 281921, 4, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 252, 620) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1446) [M::mem_pestat] mean and std.dev: (348.19, 230.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1859) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (241, 374, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045) [M::mem_pestat] mean and std.dev: (383.43, 176.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1313) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 304, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062) [M::mem_pestat] mean and std.dev: (276.39, 162.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1365) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678340 sequences (100000273 bp) in 2.795 CPU sec, 2.834 real sec... [M::process] read 678404 sequences (100000281 bp) in 2.300 CPU sec, 2.306 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 281451, 1, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 220, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (256.92, 118.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 377, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047) [M::mem_pestat] mean and std.dev: (385.07, 176.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1315) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (268, 311, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (333.92, 138.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 678684 reads in 197.698 CPU sec, 6.241 real sec [M::mem_process_seqs] Processed 678416 reads in 133.060 CPU sec, 4.214 real sec sorting block 6, #reads = 1079775 mem = 536880373 peak = 1006825472 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 281980, 0, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 256, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931) [M::mem_pestat] mean and std.dev: (302.53, 150.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1184) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 378, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045) [M::mem_pestat] mean and std.dev: (385.54, 176.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1312) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 273, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007) [M::mem_pestat] mean and std.dev: (319.26, 149.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1269) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678138 sequences (100000257 bp) in 2.821 CPU sec, 2.855 real sec... [M::process] read 678452 sequences (100000044 bp) in 2.360 CPU sec, 2.351 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 281641, 2, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 178, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (212.58, 114.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 379, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043) [M::mem_pestat] mean and std.dev: (385.68, 176.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1309) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (131, 180, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (209.26, 95.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 678340 reads in 197.387 CPU sec, 6.224 real sec sorting block 7, #reads = 1079640 mem = 536875839 peak = 1007230976 [M::mem_process_seqs] Processed 678404 reads in 130.523 CPU sec, 4.129 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 282002, 4, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 250, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044) [M::mem_pestat] mean and std.dev: (304.66, 146.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1323) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 380, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052) [M::mem_pestat] mean and std.dev: (387.68, 177.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1321) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 298, 727) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1739) [M::mem_pestat] mean and std.dev: (387.81, 218.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2245) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677778 sequences (100000048 bp) in 3.190 CPU sec, 3.240 real sec... [M::process] read 677810 sequences (100000000 bp) in 2.270 CPU sec, 2.266 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 281610, 0, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 244, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (231.96, 55.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 380, 515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055) [M::mem_pestat] mean and std.dev: (387.35, 177.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1325) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 312, 557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1219) [M::mem_pestat] mean and std.dev: (360.47, 180.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1550) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 678138 reads in 203.677 CPU sec, 6.429 real sec sorting block 8, #reads = 1078976 mem = 536878802 peak = 1016971264 [M::mem_process_seqs] Processed 678452 reads in 136.208 CPU sec, 4.310 real sec sorting block 9, #reads = 1079183 mem = 536879794 peak = 1052327936 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 281721, 5, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (324, 361, 672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1368) [M::mem_pestat] mean and std.dev: (418.18, 220.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 387, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1065) [M::mem_pestat] mean and std.dev: (392.92, 178.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 280, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1180) [M::mem_pestat] mean and std.dev: (323.41, 185.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1513) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677468 sequences (100000027 bp) in 2.897 CPU sec, 2.938 real sec... [M::process] read 677310 sequences (100000220 bp) in 2.380 CPU sec, 2.383 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 281050, 5, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 313, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (308.60, 133.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 383, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052) [M::mem_pestat] mean and std.dev: (389.83, 177.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1320) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 251, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (310.70, 177.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677778 reads in 205.189 CPU sec, 6.474 real sec [M::mem_process_seqs] Processed 677810 reads in 135.761 CPU sec, 4.302 real sec sorting block 10, #reads = 1078859 mem = 536879129 peak = 1052327936 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281539, 0, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 328, 1226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3222) [M::mem_pestat] mean and std.dev: (441.85, 358.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4220) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 382, 515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047) [M::mem_pestat] mean and std.dev: (389.16, 176.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1313) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 247, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (255.42, 119.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677050 sequences (100000207 bp) in 3.152 CPU sec, 3.189 real sec... [M::process] read 677052 sequences (100000103 bp) in 2.400 CPU sec, 2.395 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 280873, 3, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (253, 350, 572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1210) [M::mem_pestat] mean and std.dev: (341.80, 161.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (251, 383, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040) [M::mem_pestat] mean and std.dev: (389.93, 175.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1303) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 287, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (272.40, 78.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677468 reads in 201.757 CPU sec, 6.358 real sec [M::mem_process_seqs] Processed 677310 reads in 132.824 CPU sec, 4.200 real sec sorting block 11, #reads = 1078784 mem = 536878680 peak = 1052327936 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 280579, 6, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 277, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (265.13, 99.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (251, 381, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040) [M::mem_pestat] mean and std.dev: (389.13, 174.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1303) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 286, 565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1271) [M::mem_pestat] mean and std.dev: (349.06, 212.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1624) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677086 sequences (100000027 bp) in 2.955 CPU sec, 2.992 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 281091, 1, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 268, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055) [M::mem_pestat] mean and std.dev: (316.47, 194.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1357) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (250, 378, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021) [M::mem_pestat] mean and std.dev: (385.83, 172.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1278) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 230, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1063) [M::mem_pestat] mean and std.dev: (263.16, 130.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1355) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676818 sequences (100000058 bp) in 2.430 CPU sec, 2.426 real sec... [M::mem_process_seqs] Processed 677050 reads in 204.171 CPU sec, 6.450 real sec sorting block 12, #reads = 1078123 mem = 536877709 peak = 1052327936 [M::mem_process_seqs] Processed 677052 reads in 136.108 CPU sec, 4.300 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 281114, 0, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (252, 372, 1155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2961) [M::mem_pestat] mean and std.dev: (408.43, 324.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (252, 381, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035) [M::mem_pestat] mean and std.dev: (389.00, 174.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1296) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 256, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (251.11, 99.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677216 sequences (100000288 bp) in 3.347 CPU sec, 3.393 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 281289, 0, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 328, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (318.25, 128.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (253, 382, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033) [M::mem_pestat] mean and std.dev: (389.73, 174.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1293) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 336, 894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2210) [M::mem_pestat] mean and std.dev: (347.12, 243.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2868) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677372 sequences (100000276 bp) in 2.420 CPU sec, 2.421 real sec... [M::mem_process_seqs] Processed 677086 reads in 202.848 CPU sec, 6.395 real sec sorting block 13, #reads = 1077259 mem = 536878177 peak = 1052327936 [M::mem_process_seqs] Processed 676818 reads in 135.946 CPU sec, 4.288 real sec sorting block 14, #reads = 1077039 mem = 536885292 peak = 1052327936 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 281379, 1, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 364, 5222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15284) [M::mem_pestat] mean and std.dev: (1837.44, 2286.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20315) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (250, 381, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039) [M::mem_pestat] mean and std.dev: (388.49, 175.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1302) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (309, 383, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (382.42, 147.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677536 sequences (100000166 bp) in 3.028 CPU sec, 3.070 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 280973, 3, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 261, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (308.37, 192.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1204) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 374, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018) [M::mem_pestat] mean and std.dev: (382.85, 172.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1275) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (266, 331, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (295.79, 74.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677452 sequences (100000093 bp) in 2.470 CPU sec, 2.466 real sec... [M::mem_process_seqs] Processed 677216 reads in 206.802 CPU sec, 6.530 real sec [M::mem_process_seqs] Processed 677372 reads in 138.662 CPU sec, 4.379 real sec sorting block 15, #reads = 1076548 mem = 536880229 peak = 1052327936 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 281019, 1, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (255, 309, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1059) [M::mem_pestat] mean and std.dev: (297.21, 106.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1327) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 371, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012) [M::mem_pestat] mean and std.dev: (379.36, 170.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1268) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 347, 653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1493) [M::mem_pestat] mean and std.dev: (338.44, 185.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1913) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677622 sequences (100000006 bp) in 3.175 CPU sec, 3.220 real sec... [M::process] read 676644 sequences (100000292 bp) in 1.970 CPU sec, 1.966 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 280808, 4, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 174, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (220.48, 97.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 373, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017) [M::mem_pestat] mean and std.dev: (382.06, 172.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1274) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 288, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (354.07, 177.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1164) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677536 reads in 200.354 CPU sec, 6.332 real sec [M::mem_process_seqs] Processed 677452 reads in 133.972 CPU sec, 4.247 real sec sorting block 16, #reads = 1076008 mem = 536878032 peak = 1052327936 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 281865, 5, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 268, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810) [M::mem_pestat] mean and std.dev: (280.16, 121.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1016) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 372, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016) [M::mem_pestat] mean and std.dev: (381.04, 172.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1273) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (268, 352, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (325.32, 122.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677100 sequences (100000268 bp) in 2.697 CPU sec, 2.745 real sec... [M::process] read 676986 sequences (100000033 bp) in 2.010 CPU sec, 2.002 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 281580, 6, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 238, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (261.64, 124.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (254, 386, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046) [M::mem_pestat] mean and std.dev: (393.33, 176.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1310) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 279, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (271.35, 129.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677622 reads in 199.549 CPU sec, 6.326 real sec sorting block 17, #reads = 1076359 mem = 536879428 peak = 1052327936 [M::mem_process_seqs] Processed 676644 reads in 134.208 CPU sec, 4.258 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 282000, 11, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 392, 773) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1859) [M::mem_pestat] mean and std.dev: (444.45, 367.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (251, 384, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1049) [M::mem_pestat] mean and std.dev: (392.24, 177.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1315) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (750, 750, 2914) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7242) [M::mem_pestat] mean and std.dev: (1535.09, 1444.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9406) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 370, 555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1365) [M::mem_pestat] mean and std.dev: (323.17, 197.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1770) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 676668 sequences (100000144 bp) in 2.983 CPU sec, 3.022 real sec... [M::process] read 679752 sequences (100000163 bp) in 2.090 CPU sec, 2.090 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 282114, 1, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 365, 615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1513) [M::mem_pestat] mean and std.dev: (379.18, 220.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1962) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (252, 384, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050) [M::mem_pestat] mean and std.dev: (393.00, 178.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1316) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 372, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003) [M::mem_pestat] mean and std.dev: (334.83, 154.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1271) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677100 reads in 197.884 CPU sec, 6.259 real sec sorting block 18, #reads = 1075555 mem = 536878204 peak = 1052327936 [M::mem_process_seqs] Processed 676986 reads in 134.866 CPU sec, 4.262 real sec sorting block 19, #reads = 1075361 mem = 536878780 peak = 1101991936 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282054, 0, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 178, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1146) [M::mem_pestat] mean and std.dev: (236.56, 144.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (254, 390, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1067) [M::mem_pestat] mean and std.dev: (398.37, 181.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (246, 340, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (6, 606) [M::mem_pestat] mean and std.dev: (305.90, 96.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681844 sequences (100000043 bp) in 2.671 CPU sec, 2.717 real sec... [M::process] read 681040 sequences (100000014 bp) in 2.010 CPU sec, 2.007 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 283067, 3, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 276, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (226.15, 73.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (234, 367, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047) [M::mem_pestat] mean and std.dev: (378.98, 178.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1318) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 286, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026) [M::mem_pestat] mean and std.dev: (307.67, 148.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1297) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676668 reads in 197.387 CPU sec, 6.252 real sec [M::mem_process_seqs] Processed 679752 reads in 135.546 CPU sec, 4.305 real sec sorting block 20, #reads = 1074991 mem = 536878876 peak = 1101991936 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282344, 2, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 275, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (267.23, 132.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1196) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (220, 349, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030) [M::mem_pestat] mean and std.dev: (365.22, 177.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1300) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 296, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (285.69, 99.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680276 sequences (100000235 bp) in 3.429 CPU sec, 3.459 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282316, 1, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 267, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959) [M::mem_pestat] mean and std.dev: (269.87, 142.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1224) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (226, 356, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030) [M::mem_pestat] mean and std.dev: (369.86, 177.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1298) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 297, 499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045) [M::mem_pestat] mean and std.dev: (332.26, 173.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1318) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679916 sequences (100000033 bp) in 2.460 CPU sec, 2.462 real sec... [M::mem_process_seqs] Processed 681844 reads in 204.967 CPU sec, 6.464 real sec [M::mem_process_seqs] Processed 681040 reads in 135.308 CPU sec, 4.268 real sec sorting block 21, #reads = 1080915 mem = 536877703 peak = 1101991936 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 282198, 6, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 366, 535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1231) [M::mem_pestat] mean and std.dev: (306.50, 146.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (230, 361, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034) [M::mem_pestat] mean and std.dev: (374.20, 177.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1302) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 354, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066) [M::mem_pestat] mean and std.dev: (306.64, 155.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1369) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679968 sequences (100000214 bp) in 2.824 CPU sec, 2.864 real sec... [M::process] read 679746 sequences (100000105 bp) in 2.010 CPU sec, 2.008 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 282335, 0, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 321, 595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1421) [M::mem_pestat] mean and std.dev: (352.43, 192.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1834) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (232, 361, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021) [M::mem_pestat] mean and std.dev: (373.10, 175.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1284) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 255, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (241.20, 98.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680276 reads in 194.455 CPU sec, 6.164 real sec sorting block 22, #reads = 1082413 mem = 536877534 peak = 1101991936 [M::mem_process_seqs] Processed 679916 reads in 126.365 CPU sec, 4.004 real sec sorting block 23, #reads = 1082218 mem = 536878086 peak = 1101991936 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 281919, 2, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 205, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (212.52, 74.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (231, 362, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029) [M::mem_pestat] mean and std.dev: (373.30, 175.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1295) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 287, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (297.24, 142.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1155) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679764 sequences (100000153 bp) in 3.214 CPU sec, 3.257 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 282341, 6, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 255, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (239.70, 74.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (234, 365, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032) [M::mem_pestat] mean and std.dev: (376.15, 175.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1298) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 317, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951) [M::mem_pestat] mean and std.dev: (295.84, 104.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1187) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679046 sequences (100000279 bp) in 2.440 CPU sec, 2.443 real sec... [M::mem_process_seqs] Processed 679968 reads in 195.282 CPU sec, 6.158 real sec [M::mem_process_seqs] Processed 679746 reads in 129.485 CPU sec, 4.075 real sec sorting block 24, #reads = 1081906 mem = 536878269 peak = 1101991936 [M::process] read 678934 sequences (100000267 bp) in 3.083 CPU sec, 3.113 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282201, 0, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 260, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (264.68, 114.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (237, 371, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047) [M::mem_pestat] mean and std.dev: (380.94, 177.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1317) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 334, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (316.59, 178.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1030) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679248 sequences (100000040 bp) in 2.450 CPU sec, 2.446 real sec... [M::mem_process_seqs] Processed 679046 reads in 110.865 CPU sec, 3.462 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 282558, 6, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 336, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974) [M::mem_pestat] mean and std.dev: (279.31, 130.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1253) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (234, 367, 499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029) [M::mem_pestat] mean and std.dev: (376.08, 175.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1294) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 291, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (41, 536) [M::mem_pestat] mean and std.dev: (255.13, 72.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282277, 6, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 296, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960) [M::mem_pestat] mean and std.dev: (326.13, 119.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1210) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (240, 376, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1059) [M::mem_pestat] mean and std.dev: (384.79, 179.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1332) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 338, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (326.22, 155.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1194) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 679764 reads in 300.309 CPU sec, 9.477 real sec sorting block 25, #reads = 1080428 mem = 536879401 peak = 1101991936 [M::process] read 678994 sequences (100000137 bp) in 2.844 CPU sec, 2.850 real sec... [M::mem_process_seqs] Processed 678934 reads in 153.089 CPU sec, 4.834 real sec sorting block 26, #reads = 1081817 mem = 536879018 peak = 1137659904 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 281620, 1, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (262, 334, 606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1294) [M::mem_pestat] mean and std.dev: (328.19, 135.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (238, 373, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048) [M::mem_pestat] mean and std.dev: (382.04, 178.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1318) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 305, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (315.25, 118.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678646 sequences (100000042 bp) in 3.261 CPU sec, 3.350 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 281664, 1, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (239, 350, 780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1862) [M::mem_pestat] mean and std.dev: (370.97, 230.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (239, 371, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034) [M::mem_pestat] mean and std.dev: (380.03, 175.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1299) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 286, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (321.18, 156.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 679248 reads in 196.772 CPU sec, 6.220 real sec [M::process] read 678294 sequences (100000028 bp) in 2.419 CPU sec, 2.419 real sec... [M::mem_process_seqs] Processed 678994 reads in 131.701 CPU sec, 4.135 real sec sorting block 27, #reads = 1080897 mem = 536880048 peak = 1137659904 [M::process] read 678374 sequences (100000299 bp) in 3.304 CPU sec, 3.362 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 281418, 2, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 129, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (137.36, 31.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 376, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041) [M::mem_pestat] mean and std.dev: (384.09, 176.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1307) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 287, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (269.26, 107.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678254 sequences (100000146 bp) in 1.990 CPU sec, 1.995 real sec... [M::mem_process_seqs] Processed 678294 reads in 112.604 CPU sec, 3.554 real sec sorting block 28, #reads = 1079619 mem = 536881918 peak = 1137659904 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281736, 1, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 291, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (287.85, 121.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (240, 372, 506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038) [M::mem_pestat] mean and std.dev: (381.47, 176.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1304) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (253, 340, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045) [M::mem_pestat] mean and std.dev: (343.82, 167.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1309) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 282092, 5, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 333, 1931) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5359) [M::mem_pestat] mean and std.dev: (1246.49, 1621.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7732) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 376, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041) [M::mem_pestat] mean and std.dev: (384.10, 176.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1307) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 270, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (6, 511) [M::mem_pestat] mean and std.dev: (254.15, 85.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 678646 reads in 306.751 CPU sec, 9.700 real sec [M::process] read 678206 sequences (100000242 bp) in 2.441 CPU sec, 2.449 real sec... [M::mem_process_seqs] Processed 678374 reads in 157.022 CPU sec, 4.989 real sec sorting block 29, #reads = 1080256 mem = 536884372 peak = 1137659904 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 281661, 10, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (239, 286, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (317.95, 164.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 377, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042) [M::mem_pestat] mean and std.dev: (385.16, 176.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1308) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (857, 857, 857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (857, 857) [M::mem_pestat] mean and std.dev: (857.00, 0.00) [M::mem_pestat] low and high boundaries for proper pairs: (857, 857) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (277, 474, 627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1327) [M::mem_pestat] mean and std.dev: (471.38, 225.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1677) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 678196 sequences (100000098 bp) in 3.567 CPU sec, 3.630 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 281520, 5, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 243, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1108) [M::mem_pestat] mean and std.dev: (249.63, 131.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 380, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1049) [M::mem_pestat] mean and std.dev: (387.54, 177.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1317) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (254, 451, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013) [M::mem_pestat] mean and std.dev: (364.68, 153.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1266) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677936 sequences (100000140 bp) in 2.400 CPU sec, 2.408 real sec... [M::mem_process_seqs] Processed 678254 reads in 203.425 CPU sec, 6.419 real sec [M::mem_process_seqs] Processed 678206 reads in 136.608 CPU sec, 4.307 real sec sorting block 30, #reads = 1079388 mem = 536879796 peak = 1137659904 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 281356, 1, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 318, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (315.32, 139.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 380, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050) [M::mem_pestat] mean and std.dev: (387.89, 177.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1318) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 388, 713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1675) [M::mem_pestat] mean and std.dev: (462.83, 274.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2156) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678022 sequences (100000215 bp) in 3.364 CPU sec, 3.422 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 281164, 1, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 216, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (243.73, 104.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 381, 515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051) [M::mem_pestat] mean and std.dev: (389.06, 178.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1319) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 339, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965) [M::mem_pestat] mean and std.dev: (335.38, 141.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1212) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677756 sequences (100000159 bp) in 2.440 CPU sec, 2.445 real sec... [M::mem_process_seqs] Processed 678196 reads in 204.233 CPU sec, 6.440 real sec sorting block 31, #reads = 1079628 mem = 536878466 peak = 1137659904 [M::mem_process_seqs] Processed 677936 reads in 134.542 CPU sec, 4.249 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 281539, 6, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 384, 5141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14963) [M::mem_pestat] mean and std.dev: (1664.52, 2070.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19874) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 381, 515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053) [M::mem_pestat] mean and std.dev: (388.65, 178.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1322) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 268, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (273.22, 112.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677816 sequences (100000121 bp) in 3.396 CPU sec, 3.443 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 281228, 1, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (242, 341, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (313.33, 110.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 379, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036) [M::mem_pestat] mean and std.dev: (386.52, 175.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1299) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (250, 309, 640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1420) [M::mem_pestat] mean and std.dev: (372.51, 188.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1810) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677448 sequences (100000084 bp) in 2.430 CPU sec, 2.429 real sec... [M::mem_process_seqs] Processed 678022 reads in 202.577 CPU sec, 6.392 real sec sorting block 32, #reads = 1078990 mem = 536879307 peak = 1137659904 [M::mem_process_seqs] Processed 677756 reads in 135.427 CPU sec, 4.265 real sec sorting block 33, #reads = 1078528 mem = 536875730 peak = 1146896384 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 281860, 1, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 243, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (223.64, 75.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 379, 512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042) [M::mem_pestat] mean and std.dev: (387.13, 176.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1307) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 304, 2523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7213) [M::mem_pestat] mean and std.dev: (1284.76, 1911.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9558) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677508 sequences (100000012 bp) in 3.167 CPU sec, 3.237 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282065, 0, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 224, 564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1424) [M::mem_pestat] mean and std.dev: (320.60, 227.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1854) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 381, 515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047) [M::mem_pestat] mean and std.dev: (389.68, 177.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1313) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (352, 486, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (412.57, 135.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677300 sequences (100000255 bp) in 2.500 CPU sec, 2.493 real sec... [M::mem_process_seqs] Processed 677816 reads in 208.193 CPU sec, 6.570 real sec [M::mem_process_seqs] Processed 677448 reads in 140.113 CPU sec, 4.429 real sec sorting block 34, #reads = 1078070 mem = 536879526 peak = 1146896384 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282039, 5, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 220, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (227.67, 89.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 378, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039) [M::mem_pestat] mean and std.dev: (386.55, 175.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1303) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 260, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (245.11, 102.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677628 sequences (100000273 bp) in 3.201 CPU sec, 3.255 real sec... [M::process] read 677834 sequences (100000266 bp) in 1.990 CPU sec, 1.989 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 281512, 3, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 319, 681) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1573) [M::mem_pestat] mean and std.dev: (396.93, 270.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2019) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 379, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045) [M::mem_pestat] mean and std.dev: (387.64, 176.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1311) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 275, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (252.30, 108.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677508 reads in 202.900 CPU sec, 6.417 real sec [M::mem_process_seqs] Processed 677300 reads in 132.830 CPU sec, 4.212 real sec sorting block 35, #reads = 1078693 mem = 536880978 peak = 1146896384 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281651, 1, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 261, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056) [M::mem_pestat] mean and std.dev: (254.14, 159.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 377, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043) [M::mem_pestat] mean and std.dev: (385.92, 176.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1309) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (143, 411, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001) [M::mem_pestat] mean and std.dev: (320.54, 153.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1287) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677388 sequences (100000283 bp) in 3.402 CPU sec, 3.450 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 281693, 1, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 284, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033) [M::mem_pestat] mean and std.dev: (303.67, 164.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1321) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 375, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040) [M::mem_pestat] mean and std.dev: (386.00, 177.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1305) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 235, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (61, 451) [M::mem_pestat] mean and std.dev: (239.03, 66.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677494 sequences (100000125 bp) in 2.450 CPU sec, 2.446 real sec... [M::mem_process_seqs] Processed 677628 reads in 197.899 CPU sec, 6.236 real sec sorting block 36, #reads = 1078600 mem = 536877489 peak = 1146896384 [M::mem_process_seqs] Processed 677834 reads in 130.810 CPU sec, 4.126 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 281484, 3, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 217, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (227.50, 85.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 374, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029) [M::mem_pestat] mean and std.dev: (384.40, 175.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1290) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 368, 478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962) [M::mem_pestat] mean and std.dev: (346.54, 147.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1204) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677374 sequences (100000069 bp) in 3.375 CPU sec, 3.407 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 281411, 1, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 255, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (262.61, 132.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 372, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025) [M::mem_pestat] mean and std.dev: (383.23, 175.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1285) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 244, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (234.48, 103.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677322 sequences (100000237 bp) in 2.040 CPU sec, 2.040 real sec... [M::mem_process_seqs] Processed 677388 reads in 192.732 CPU sec, 6.082 real sec sorting block 37, #reads = 1078444 mem = 536884747 peak = 1146896384 [M::mem_process_seqs] Processed 677494 reads in 129.895 CPU sec, 4.106 real sec sorting block 38, #reads = 1078119 mem = 536881575 peak = 1221058560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 281968, 1, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 391, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (350.23, 158.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 377, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037) [M::mem_pestat] mean and std.dev: (387.42, 176.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1300) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 334, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (324.64, 142.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677126 sequences (100000286 bp) in 3.462 CPU sec, 3.502 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 282244, 3, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 443, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1163) [M::mem_pestat] mean and std.dev: (374.62, 174.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1476) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 378, 512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040) [M::mem_pestat] mean and std.dev: (388.18, 177.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1304) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (256, 356, 652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1444) [M::mem_pestat] mean and std.dev: (407.53, 199.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1840) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677374 reads in 194.714 CPU sec, 6.137 real sec [M::process] read 676840 sequences (100000272 bp) in 2.400 CPU sec, 2.407 real sec... [M::mem_process_seqs] Processed 677322 reads in 134.587 CPU sec, 4.237 real sec sorting block 39, #reads = 1077392 mem = 536879078 peak = 1221058560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 281878, 5, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 275, 612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1412) [M::mem_pestat] mean and std.dev: (396.64, 285.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1812) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (251, 386, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1064) [M::mem_pestat] mean and std.dev: (394.91, 180.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1335) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 221, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (235.11, 84.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676734 sequences (100000221 bp) in 3.434 CPU sec, 3.465 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282514, 1, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 417, 596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1316) [M::mem_pestat] mean and std.dev: (426.82, 224.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1676) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (254, 388, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1061) [M::mem_pestat] mean and std.dev: (396.82, 181.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1330) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 349, 669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1615) [M::mem_pestat] mean and std.dev: (399.71, 241.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2088) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680432 sequences (100000078 bp) in 2.456 CPU sec, 2.462 real sec... [M::mem_process_seqs] Processed 677126 reads in 198.515 CPU sec, 6.266 real sec [M::mem_process_seqs] Processed 676840 reads in 137.318 CPU sec, 4.343 real sec sorting block 40, #reads = 1078368 mem = 536881013 peak = 1221058560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 282312, 0, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 410, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1144) [M::mem_pestat] mean and std.dev: (375.78, 165.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (253, 388, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1063) [M::mem_pestat] mean and std.dev: (396.65, 181.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 352, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948) [M::mem_pestat] mean and std.dev: (302.14, 104.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1190) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682296 sequences (100000162 bp) in 3.702 CPU sec, 3.803 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 283841, 3, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 367, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (317.88, 122.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1067) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (229, 358, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030) [M::mem_pestat] mean and std.dev: (372.38, 178.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1297) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 219, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (248.00, 101.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676734 reads in 207.849 CPU sec, 6.621 real sec [M::process] read 681146 sequences (100000101 bp) in 2.490 CPU sec, 2.497 real sec... sorting block 41, #reads = 1078471 mem = 536876796 peak = 1221058560 [M::mem_process_seqs] Processed 680432 reads in 144.838 CPU sec, 4.599 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 284073, 3, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 241, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (313.12, 172.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1168) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (218, 343, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010) [M::mem_pestat] mean and std.dev: (361.12, 176.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1274) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 352, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989) [M::mem_pestat] mean and std.dev: (322.49, 167.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1261) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680214 sequences (100000018 bp) in 3.683 CPU sec, 3.820 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283474, 4, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 284, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (280.00, 91.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (226, 355, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027) [M::mem_pestat] mean and std.dev: (369.71, 177.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1294) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 266, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1117) [M::mem_pestat] mean and std.dev: (271.11, 135.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1429) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682296 reads in 209.037 CPU sec, 6.701 real sec [M::process] read 679716 sequences (100000180 bp) in 2.537 CPU sec, 2.550 real sec... sorting block 42, #reads = 1080474 mem = 536878005 peak = 1221058560 [M::mem_process_seqs] Processed 681146 reads in 142.138 CPU sec, 4.525 real sec sorting block 43, #reads = 1083235 mem = 536877691 peak = 1227935744 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 283229, 1, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 206, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921) [M::mem_pestat] mean and std.dev: (270.56, 162.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1168) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (231, 361, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029) [M::mem_pestat] mean and std.dev: (373.84, 177.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1295) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 409, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048) [M::mem_pestat] mean and std.dev: (370.30, 170.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1324) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679620 sequences (100000197 bp) in 3.663 CPU sec, 3.752 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 282468, 7, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 245, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (248.11, 100.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (235, 366, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030) [M::mem_pestat] mean and std.dev: (377.09, 177.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1295) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 302, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (294.30, 135.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680214 reads in 205.474 CPU sec, 6.541 real sec [M::process] read 678956 sequences (100000026 bp) in 2.365 CPU sec, 2.390 real sec... [M::mem_process_seqs] Processed 679716 reads in 140.967 CPU sec, 4.481 real sec sorting block 44, #reads = 1080348 mem = 536880152 peak = 1227935744 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282604, 4, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 247, 723) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1821) [M::mem_pestat] mean and std.dev: (357.51, 314.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2370) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (236, 367, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034) [M::mem_pestat] mean and std.dev: (378.57, 177.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1300) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 225, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (259.80, 96.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679080 sequences (100000163 bp) in 3.597 CPU sec, 3.652 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282693, 3, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 475, 944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2368) [M::mem_pestat] mean and std.dev: (520.23, 436.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3080) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (238, 371, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045) [M::mem_pestat] mean and std.dev: (382.20, 179.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1314) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 445, 583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1303) [M::mem_pestat] mean and std.dev: (387.93, 194.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1663) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678338 sequences (100000253 bp) in 2.275 CPU sec, 2.285 real sec... [M::mem_process_seqs] Processed 679620 reads in 204.645 CPU sec, 6.498 real sec [M::mem_process_seqs] Processed 678956 reads in 139.277 CPU sec, 4.408 real sec sorting block 45, #reads = 1081551 mem = 536878558 peak = 1227935744 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282954, 1, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 214, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (202.29, 60.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (240, 375, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062) [M::mem_pestat] mean and std.dev: (385.82, 181.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1336) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (272, 338, 627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1337) [M::mem_pestat] mean and std.dev: (359.86, 175.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1692) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678464 sequences (100000242 bp) in 3.461 CPU sec, 3.552 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 282699, 2, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (284, 375, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (345.29, 127.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 378, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054) [M::mem_pestat] mean and std.dev: (387.96, 179.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1324) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 263, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (277.79, 132.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678328 sequences (100000036 bp) in 2.184 CPU sec, 2.193 real sec... [M::mem_process_seqs] Processed 679080 reads in 198.932 CPU sec, 6.312 real sec sorting block 46, #reads = 1080888 mem = 536878942 peak = 1227935744 [M::mem_process_seqs] Processed 678338 reads in 136.197 CPU sec, 4.311 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282806, 5, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 280, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (263.48, 119.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1188) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 380, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1063) [M::mem_pestat] mean and std.dev: (389.63, 181.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1336) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 381, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1133) [M::mem_pestat] mean and std.dev: (344.54, 144.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1436) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 47, #reads = 1080838 mem = 536881558 peak = 1227935744 [M::process] read 677934 sequences (100000297 bp) in 3.637 CPU sec, 3.739 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 282468, 2, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 318, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1138) [M::mem_pestat] mean and std.dev: (330.29, 207.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 383, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075) [M::mem_pestat] mean and std.dev: (391.79, 182.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1352) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (254, 287, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956) [M::mem_pestat] mean and std.dev: (309.57, 118.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1190) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 678464 reads in 201.667 CPU sec, 6.387 real sec [M::process] read 677888 sequences (100000179 bp) in 2.405 CPU sec, 2.431 real sec... [M::mem_process_seqs] Processed 678328 reads in 140.677 CPU sec, 4.437 real sec sorting block 48, #reads = 1081021 mem = 536881968 peak = 1227935744 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 282168, 2, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 336, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041) [M::mem_pestat] mean and std.dev: (342.39, 157.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1324) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 387, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076) [M::mem_pestat] mean and std.dev: (395.45, 183.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1352) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (121, 192, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1174) [M::mem_pestat] mean and std.dev: (325.59, 241.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1525) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677728 sequences (100000149 bp) in 3.566 CPU sec, 3.657 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 282163, 7, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 245, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (244.43, 97.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 385, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1070) [M::mem_pestat] mean and std.dev: (393.65, 182.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1344) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 340, 669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1581) [M::mem_pestat] mean and std.dev: (370.08, 215.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2037) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677782 sequences (100000183 bp) in 2.472 CPU sec, 2.476 real sec... [M::mem_process_seqs] Processed 677934 reads in 203.226 CPU sec, 6.424 real sec [M::mem_process_seqs] Processed 677888 reads in 137.720 CPU sec, 4.347 real sec sorting block 49, #reads = 1079205 mem = 536878778 peak = 1227935744 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282348, 4, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 292, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1273) [M::mem_pestat] mean and std.dev: (289.27, 198.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 386, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1071) [M::mem_pestat] mean and std.dev: (395.17, 182.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1345) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 263, 542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1258) [M::mem_pestat] mean and std.dev: (295.15, 161.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1616) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678894 sequences (100000051 bp) in 3.283 CPU sec, 3.357 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282411, 0, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 295, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (289.79, 130.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 386, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074) [M::mem_pestat] mean and std.dev: (395.01, 183.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1349) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 251, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1102) [M::mem_pestat] mean and std.dev: (312.89, 169.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1407) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677518 sequences (100000014 bp) in 2.310 CPU sec, 2.305 real sec... [M::mem_process_seqs] Processed 677728 reads in 205.083 CPU sec, 6.504 real sec [M::mem_process_seqs] Processed 677782 reads in 142.469 CPU sec, 4.510 real sec sorting block 50, #reads = 1079742 mem = 536878703 peak = 1227935744 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282759, 11, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 313, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992) [M::mem_pestat] mean and std.dev: (304.79, 131.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1245) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (238, 374, 515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1069) [M::mem_pestat] mean and std.dev: (386.06, 182.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1346) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (687, 687, 904) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (253, 1338) [M::mem_pestat] mean and std.dev: (715.56, 179.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1555) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 277, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008) [M::mem_pestat] mean and std.dev: (327.88, 143.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1274) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 677318 sequences (100000184 bp) in 3.374 CPU sec, 3.443 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 282619, 6, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 164, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (199.50, 103.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 387, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1080) [M::mem_pestat] mean and std.dev: (396.49, 183.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1357) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (340, 446, 812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1756) [M::mem_pestat] mean and std.dev: (456.27, 219.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2228) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677252 sequences (100000283 bp) in 2.323 CPU sec, 2.320 real sec... [M::mem_process_seqs] Processed 678894 reads in 205.163 CPU sec, 6.497 real sec sorting block 51, #reads = 1080465 mem = 536882606 peak = 1227935744 [M::mem_process_seqs] Processed 677518 reads in 137.283 CPU sec, 4.341 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 281403, 3, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 254, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1151) [M::mem_pestat] mean and std.dev: (340.67, 214.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (251, 387, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1073) [M::mem_pestat] mean and std.dev: (396.87, 183.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1347) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 271, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (311.15, 170.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 52, #reads = 1079898 mem = 536879051 peak = 1261953024 [M::process] read 677274 sequences (100000230 bp) in 3.759 CPU sec, 3.852 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282110, 1, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (227, 309, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (298.33, 136.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (250, 383, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060) [M::mem_pestat] mean and std.dev: (393.50, 180.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1330) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (304, 404, 733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1591) [M::mem_pestat] mean and std.dev: (469.73, 277.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2020) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677318 reads in 207.121 CPU sec, 6.554 real sec [M::process] read 676904 sequences (100000010 bp) in 2.575 CPU sec, 2.592 real sec... [M::mem_process_seqs] Processed 677252 reads in 140.847 CPU sec, 4.435 real sec sorting block 53, #reads = 1080058 mem = 536880010 peak = 1261953024 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 281705, 0, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 297, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1104) [M::mem_pestat] mean and std.dev: (300.15, 146.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (251, 386, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1064) [M::mem_pestat] mean and std.dev: (394.80, 181.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1335) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 224, 572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1380) [M::mem_pestat] mean and std.dev: (302.39, 180.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1784) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677016 sequences (100000047 bp) in 3.620 CPU sec, 3.697 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 281373, 4, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 256, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (300.06, 101.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (254, 389, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1070) [M::mem_pestat] mean and std.dev: (398.53, 182.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1342) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 225, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (222.23, 79.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676994 sequences (100000094 bp) in 2.134 CPU sec, 2.144 real sec... [M::mem_process_seqs] Processed 677274 reads in 208.446 CPU sec, 6.603 real sec [M::mem_process_seqs] Processed 676904 reads in 142.502 CPU sec, 4.509 real sec sorting block 54, #reads = 1078503 mem = 536878508 peak = 1261953024 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 282011, 0, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 201, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (217.81, 72.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (251, 385, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1064) [M::mem_pestat] mean and std.dev: (394.91, 181.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1335) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 361, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (327.90, 143.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676898 sequences (100000033 bp) in 3.558 CPU sec, 3.632 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281628, 3, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 250, 678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1640) [M::mem_pestat] mean and std.dev: (290.92, 172.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2121) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (250, 382, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1057) [M::mem_pestat] mean and std.dev: (393.26, 180.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1326) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 270, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (288.68, 118.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1068) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677438 sequences (100000117 bp) in 2.304 CPU sec, 2.304 real sec... [M::mem_process_seqs] Processed 677016 reads in 206.177 CPU sec, 6.532 real sec [M::mem_process_seqs] Processed 676994 reads in 140.728 CPU sec, 4.446 real sec sorting block 55, #reads = 1078317 mem = 536880728 peak = 1261953024 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281676, 5, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (270, 381, 536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068) [M::mem_pestat] mean and std.dev: (399.38, 197.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1334) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 383, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068) [M::mem_pestat] mean and std.dev: (394.44, 182.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 279, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1091) [M::mem_pestat] mean and std.dev: (292.85, 147.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1401) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677528 sequences (100000208 bp) in 3.224 CPU sec, 3.311 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 281817, 0, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 230, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (278.93, 155.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 380, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1057) [M::mem_pestat] mean and std.dev: (390.95, 180.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1327) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 299, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1157) [M::mem_pestat] mean and std.dev: (268.32, 118.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1473) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677614 sequences (100000081 bp) in 2.144 CPU sec, 2.141 real sec... [M::mem_process_seqs] Processed 676898 reads in 206.099 CPU sec, 6.539 real sec sorting block 56, #reads = 1078752 mem = 536878337 peak = 1261953024 [M::mem_process_seqs] Processed 677438 reads in 140.861 CPU sec, 4.464 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 281713, 3, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 272, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (286.82, 137.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] analyzing insert size distribution for orientation FR... sorting block 57, #reads = 1078092 mem = 536882333 peak = 1261953024 [M::mem_pestat] (25, 50, 75) percentile: (246, 380, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1065) [M::mem_pestat] mean and std.dev: (391.73, 182.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 287, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (295.67, 137.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677308 sequences (100000047 bp) in 3.567 CPU sec, 3.631 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282383, 1, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 217, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983) [M::mem_pestat] mean and std.dev: (196.15, 110.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1271) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 380, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1067) [M::mem_pestat] mean and std.dev: (391.53, 182.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 376, 614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1436) [M::mem_pestat] mean and std.dev: (412.60, 246.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1847) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677224 sequences (100000196 bp) in 2.300 CPU sec, 2.297 real sec... [M::mem_process_seqs] Processed 677528 reads in 208.512 CPU sec, 6.608 real sec [M::mem_process_seqs] Processed 677614 reads in 142.831 CPU sec, 4.523 real sec sorting block 58, #reads = 1077894 mem = 536879950 peak = 1261953024 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 281997, 1, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 243, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011) [M::mem_pestat] mean and std.dev: (282.47, 154.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1284) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (250, 384, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066) [M::mem_pestat] mean and std.dev: (394.56, 182.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 224, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (228.19, 110.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676964 sequences (100000035 bp) in 3.517 CPU sec, 3.576 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 281441, 3, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (248, 279, 2385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6659) [M::mem_pestat] mean and std.dev: (1253.00, 1672.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8796) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (250, 381, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054) [M::mem_pestat] mean and std.dev: (392.37, 180.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1322) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 312, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (294.22, 93.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676874 sequences (100000248 bp) in 2.387 CPU sec, 2.402 real sec... [M::mem_process_seqs] Processed 677308 reads in 205.801 CPU sec, 6.518 real sec [M::mem_process_seqs] Processed 677224 reads in 141.313 CPU sec, 4.468 real sec sorting block 59, #reads = 1078185 mem = 536880140 peak = 1261953024 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282265, 3, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 215, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (234.03, 101.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (252, 383, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050) [M::mem_pestat] mean and std.dev: (393.70, 180.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1316) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 265, 550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1264) [M::mem_pestat] mean and std.dev: (285.00, 129.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1621) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676792 sequences (100000097 bp) in 3.600 CPU sec, 3.658 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282124, 4, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 241, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923) [M::mem_pestat] mean and std.dev: (289.56, 134.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1171) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (253, 386, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066) [M::mem_pestat] mean and std.dev: (396.61, 182.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 411, 609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1361) [M::mem_pestat] mean and std.dev: (361.90, 156.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1737) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676566 sequences (100000260 bp) in 2.490 CPU sec, 2.488 real sec... [M::mem_process_seqs] Processed 676964 reads in 202.798 CPU sec, 6.415 real sec sorting block 60, #reads = 1077776 mem = 536877756 peak = 1261953024 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 282210, 3, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 224, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (213.71, 53.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (255, 389, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068) [M::mem_pestat] mean and std.dev: (399.18, 183.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1339) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 318, 690) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1656) [M::mem_pestat] mean and std.dev: (393.94, 256.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2139) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677136 sequences (100000254 bp) in 2.914 CPU sec, 2.931 real sec... [M::mem_process_seqs] Processed 676792 reads in 112.317 CPU sec, 3.545 real sec [M::mem_process_seqs] Processed 676874 reads in 301.428 CPU sec, 9.561 real sec sorting block 61, #reads = 1078584 mem = 536878433 peak = 1261953024 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 281720, 4, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 219, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (246.79, 114.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1075) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (255, 391, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074) [M::mem_pestat] mean and std.dev: (401.28, 185.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1347) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 324, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (281.02, 128.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681708 sequences (100000119 bp) in 3.674 CPU sec, 3.751 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282064, 4, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 342, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1168) [M::mem_pestat] mean and std.dev: (317.21, 156.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (254, 392, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1082) [M::mem_pestat] mean and std.dev: (401.36, 185.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1358) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 321, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (278.32, 72.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676566 reads in 229.165 CPU sec, 7.293 real sec [M::mem_process_seqs] Processed 677136 reads in 147.871 CPU sec, 4.676 real sec sorting block 62, #reads = 1076921 mem = 536883420 peak = 1261953024 [M::process] read 682292 sequences (100000109 bp) in 2.792 CPU sec, 2.818 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 283543, 3, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 241, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (262.85, 169.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1183) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (221, 351, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037) [M::mem_pestat] mean and std.dev: (368.34, 181.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1309) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 263, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (311.50, 162.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1191) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680452 sequences (100000168 bp) in 3.297 CPU sec, 3.416 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283520, 2, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 244, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (296.38, 156.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1009) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (217, 345, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033) [M::mem_pestat] mean and std.dev: (364.87, 181.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1305) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 249, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936) [M::mem_pestat] mean and std.dev: (274.57, 108.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1183) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681708 reads in 208.861 CPU sec, 6.636 real sec [M::process] read 679228 sequences (100000065 bp) in 2.399 CPU sec, 2.404 real sec... sorting block 63, #reads = 1075422 mem = 536879948 peak = 1261953024 [M::mem_process_seqs] Processed 682292 reads in 142.125 CPU sec, 4.495 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282754, 2, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (227, 443, 771) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1859) [M::mem_pestat] mean and std.dev: (430.44, 240.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (229, 363, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1063) [M::mem_pestat] mean and std.dev: (379.02, 184.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (264, 354, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (346.58, 126.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 64, #reads = 1083955 mem = 536878794 peak = 1261953024 [M::process] read 679258 sequences (100000217 bp) in 3.581 CPU sec, 3.666 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282505, 5, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 212, 590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1448) [M::mem_pestat] mean and std.dev: (264.94, 166.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1877) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (236, 371, 512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1064) [M::mem_pestat] mean and std.dev: (384.53, 183.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1340) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 281, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008) [M::mem_pestat] mean and std.dev: (345.19, 179.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1277) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680452 reads in 205.103 CPU sec, 6.518 real sec [M::process] read 678814 sequences (100000236 bp) in 2.468 CPU sec, 2.467 real sec... [M::mem_process_seqs] Processed 679228 reads in 140.849 CPU sec, 4.457 real sec sorting block 65, #reads = 1083085 mem = 536880064 peak = 1261953024 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 283211, 1, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 277, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1195) [M::mem_pestat] mean and std.dev: (321.51, 159.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (238, 375, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1072) [M::mem_pestat] mean and std.dev: (387.01, 184.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1350) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 360, 1335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3551) [M::mem_pestat] mean and std.dev: (483.95, 449.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4659) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678654 sequences (100000184 bp) in 3.439 CPU sec, 3.487 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282511, 5, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 215, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (217.40, 79.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (241, 378, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1072) [M::mem_pestat] mean and std.dev: (389.63, 184.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1349) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 306, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (311.59, 109.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678490 sequences (100000141 bp) in 2.433 CPU sec, 2.438 real sec... [M::mem_process_seqs] Processed 679258 reads in 200.312 CPU sec, 6.345 real sec [M::mem_process_seqs] Processed 678814 reads in 136.777 CPU sec, 4.320 real sec sorting block 66, #reads = 1082225 mem = 536878047 peak = 1261953024 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282719, 0, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 283, 635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1479) [M::mem_pestat] mean and std.dev: (363.04, 243.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1901) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (242, 379, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1067) [M::mem_pestat] mean and std.dev: (389.62, 183.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1342) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 283, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (292.21, 110.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678064 sequences (100000299 bp) in 3.458 CPU sec, 3.596 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282443, 0, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (266, 399, 571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1181) [M::mem_pestat] mean and std.dev: (358.87, 162.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 380, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075) [M::mem_pestat] mean and std.dev: (391.60, 184.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1352) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (281, 335, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022) [M::mem_pestat] mean and std.dev: (308.00, 119.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1269) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677680 sequences (100000079 bp) in 2.311 CPU sec, 2.318 real sec... [M::mem_process_seqs] Processed 678654 reads in 199.215 CPU sec, 6.321 real sec sorting block 67, #reads = 1082186 mem = 536881338 peak = 1261953024 [M::mem_process_seqs] Processed 678490 reads in 138.675 CPU sec, 4.392 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 283063, 3, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 253, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (248.30, 79.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 385, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1080) [M::mem_pestat] mean and std.dev: (395.01, 185.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1358) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 287, 1113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2945) [M::mem_pestat] mean and std.dev: (333.91, 294.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3861) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677850 sequences (100000248 bp) in 3.499 CPU sec, 3.569 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 281728, 0, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 310, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963) [M::mem_pestat] mean and std.dev: (343.05, 184.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1219) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 388, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1089) [M::mem_pestat] mean and std.dev: (398.21, 185.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1369) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 278, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1064) [M::mem_pestat] mean and std.dev: (307.30, 136.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1352) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677576 sequences (100000145 bp) in 2.401 CPU sec, 2.408 real sec... [M::mem_process_seqs] Processed 678064 reads in 201.582 CPU sec, 6.386 real sec sorting block 68, #reads = 1080515 mem = 536879437 peak = 1261953024 [M::mem_process_seqs] Processed 677680 reads in 138.941 CPU sec, 4.390 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282238, 3, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (249, 366, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (352.71, 119.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1093) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (250, 390, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1087) [M::mem_pestat] mean and std.dev: (398.92, 185.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1366) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 362, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (321.18, 147.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 69, #reads = 1081132 mem = 536879357 peak = 1310494720 [M::process] read 677178 sequences (100000059 bp) in 3.321 CPU sec, 3.420 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 281907, 3, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 235, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (228.15, 77.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (250, 390, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1087) [M::mem_pestat] mean and std.dev: (398.72, 184.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1366) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (268, 365, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (332.32, 100.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676974 sequences (100000224 bp) in 2.336 CPU sec, 2.348 real sec... [M::mem_process_seqs] Processed 677850 reads in 203.744 CPU sec, 6.464 real sec [M::mem_process_seqs] Processed 677576 reads in 139.850 CPU sec, 4.416 real sec sorting block 70, #reads = 1079995 mem = 536879427 peak = 1310494720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281427, 11, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (255, 286, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (308.65, 114.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (252, 390, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1086) [M::mem_pestat] mean and std.dev: (399.56, 184.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1364) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (649, 745, 745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (457, 937) [M::mem_pestat] mean and std.dev: (697.00, 48.00) [M::mem_pestat] low and high boundaries for proper pairs: (361, 1033) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 373, 612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1380) [M::mem_pestat] mean and std.dev: (408.67, 264.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1764) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 677556 sequences (100000079 bp) in 3.589 CPU sec, 3.665 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 282418, 5, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 309, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (319.48, 161.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (253, 391, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1084) [M::mem_pestat] mean and std.dev: (399.66, 184.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1361) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 314, 600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1372) [M::mem_pestat] mean and std.dev: (372.19, 246.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1758) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678686 sequences (100000009 bp) in 2.370 CPU sec, 2.381 real sec... [M::mem_process_seqs] Processed 677178 reads in 205.450 CPU sec, 6.504 real sec [M::mem_process_seqs] Processed 676974 reads in 142.069 CPU sec, 4.491 real sec sorting block 71, #reads = 1079807 mem = 536883424 peak = 1310494720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 282365, 3, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (259, 342, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1057) [M::mem_pestat] mean and std.dev: (291.16, 103.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1323) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (251, 389, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1079) [M::mem_pestat] mean and std.dev: (397.72, 183.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (271, 364, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (324.29, 111.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677308 sequences (100000046 bp) in 3.482 CPU sec, 3.604 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282524, 4, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 251, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (252.10, 95.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (240, 375, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068) [M::mem_pestat] mean and std.dev: (387.88, 183.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1344) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 296, 793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1999) [M::mem_pestat] mean and std.dev: (416.54, 280.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2602) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676840 sequences (100000126 bp) in 2.197 CPU sec, 2.213 real sec... [M::mem_process_seqs] Processed 677556 reads in 204.635 CPU sec, 6.492 real sec sorting block 72, #reads = 1078913 mem = 536886542 peak = 1310494720 [M::mem_process_seqs] Processed 678686 reads in 141.524 CPU sec, 4.474 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281825, 3, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 308, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024) [M::mem_pestat] mean and std.dev: (336.93, 188.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1292) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (252, 389, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1077) [M::mem_pestat] mean and std.dev: (397.95, 183.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1352) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 314, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784) [M::mem_pestat] mean and std.dev: (270.13, 95.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676752 sequences (100000233 bp) in 3.264 CPU sec, 3.334 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281572, 0, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 319, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1177) [M::mem_pestat] mean and std.dev: (272.15, 163.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (255, 392, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074) [M::mem_pestat] mean and std.dev: (400.00, 183.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1347) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 355, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (311.72, 136.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1025) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676960 sequences (100000104 bp) in 2.356 CPU sec, 2.366 real sec... [M::mem_process_seqs] Processed 677308 reads in 206.135 CPU sec, 6.545 real sec sorting block 73, #reads = 1079625 mem = 536879722 peak = 1310494720 [M::mem_process_seqs] Processed 676840 reads in 141.327 CPU sec, 4.472 real sec sorting block 74, #reads = 1078355 mem = 536881320 peak = 1310724096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 281577, 3, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 372, 657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1603) [M::mem_pestat] mean and std.dev: (307.62, 212.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2076) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (255, 391, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074) [M::mem_pestat] mean and std.dev: (399.50, 182.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1347) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 354, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037) [M::mem_pestat] mean and std.dev: (366.60, 135.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1302) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676928 sequences (100000295 bp) in 3.392 CPU sec, 3.496 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 281666, 5, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 292, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (263.45, 72.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (251, 383, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055) [M::mem_pestat] mean and std.dev: (393.04, 179.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1323) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 326, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954) [M::mem_pestat] mean and std.dev: (336.19, 163.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1204) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676872 sequences (100000041 bp) in 2.544 CPU sec, 2.567 real sec... [M::mem_process_seqs] Processed 676752 reads in 207.008 CPU sec, 6.565 real sec [M::mem_process_seqs] Processed 676960 reads in 140.805 CPU sec, 4.452 real sec sorting block 75, #reads = 1079697 mem = 536878181 peak = 1310724096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 281696, 2, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 265, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017) [M::mem_pestat] mean and std.dev: (297.89, 128.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1295) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (251, 382, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046) [M::mem_pestat] mean and std.dev: (391.51, 178.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1311) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 393, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937) [M::mem_pestat] mean and std.dev: (350.74, 140.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1169) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677666 sequences (100000008 bp) in 3.570 CPU sec, 3.688 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 281540, 6, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 432, 1245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3325) [M::mem_pestat] mean and std.dev: (420.96, 439.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4365) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (251, 381, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040) [M::mem_pestat] mean and std.dev: (390.57, 177.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1303) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 354, 566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1250) [M::mem_pestat] mean and std.dev: (347.07, 153.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1592) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677182 sequences (100000121 bp) in 2.322 CPU sec, 2.340 real sec... [M::mem_process_seqs] Processed 676928 reads in 206.895 CPU sec, 6.555 real sec [M::mem_process_seqs] Processed 676872 reads in 141.878 CPU sec, 4.493 real sec sorting block 76, #reads = 1078145 mem = 536878196 peak = 1310724096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282071, 1, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 228, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950) [M::mem_pestat] mean and std.dev: (273.08, 140.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1201) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 374, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034) [M::mem_pestat] mean and std.dev: (385.10, 176.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1297) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (300, 392, 556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068) [M::mem_pestat] mean and std.dev: (385.30, 145.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1324) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677202 sequences (100000253 bp) in 3.591 CPU sec, 3.692 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281499, 2, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 312, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (358.30, 180.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1079) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 376, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039) [M::mem_pestat] mean and std.dev: (387.41, 177.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1303) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 289, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008) [M::mem_pestat] mean and std.dev: (305.11, 148.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1277) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677062 sequences (100000280 bp) in 2.470 CPU sec, 2.469 real sec... [M::mem_process_seqs] Processed 677666 reads in 205.303 CPU sec, 6.503 real sec sorting block 77, #reads = 1077742 mem = 536879100 peak = 1310724096 [M::mem_process_seqs] Processed 677182 reads in 140.538 CPU sec, 4.434 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 281746, 2, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (343, 500, 631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1207) [M::mem_pestat] mean and std.dev: (478.44, 217.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 379, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046) [M::mem_pestat] mean and std.dev: (389.46, 178.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1312) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (238, 302, 539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1141) [M::mem_pestat] mean and std.dev: (373.69, 225.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1442) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677312 sequences (100000260 bp) in 3.562 CPU sec, 3.663 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 281907, 8, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 237, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787) [M::mem_pestat] mean and std.dev: (249.13, 132.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 998) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 379, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046) [M::mem_pestat] mean and std.dev: (389.82, 178.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1312) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (251, 396, 1166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2996) [M::mem_pestat] mean and std.dev: (443.50, 338.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3911) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677190 sequences (100000289 bp) in 2.370 CPU sec, 2.377 real sec... [M::mem_process_seqs] Processed 677202 reads in 206.208 CPU sec, 6.540 real sec sorting block 78, #reads = 1078534 mem = 536878631 peak = 1310724096 [M::mem_process_seqs] Processed 677062 reads in 141.399 CPU sec, 4.463 real sec sorting block 79, #reads = 1077530 mem = 536880538 peak = 1314324480 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282054, 1, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 243, 2421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6911) [M::mem_pestat] mean and std.dev: (1156.00, 1752.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9156) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 379, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046) [M::mem_pestat] mean and std.dev: (389.13, 178.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1312) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 256, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (249.83, 123.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677248 sequences (100000064 bp) in 3.603 CPU sec, 3.670 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 281827, 10, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 303, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1223) [M::mem_pestat] mean and std.dev: (306.28, 151.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 381, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053) [M::mem_pestat] mean and std.dev: (391.87, 180.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1321) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (243, 1056, 7680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 22554) [M::mem_pestat] mean and std.dev: (2646.90, 3316.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 29991) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (274, 374, 591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1225) [M::mem_pestat] mean and std.dev: (362.24, 174.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1542) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677312 reads in 205.333 CPU sec, 6.512 real sec [M::process] read 677008 sequences (100000160 bp) in 2.475 CPU sec, 2.476 real sec... [M::mem_process_seqs] Processed 677190 reads in 142.492 CPU sec, 4.503 real sec sorting block 80, #reads = 1077478 mem = 536877182 peak = 1314324480 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 281900, 10, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 219, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (210.65, 80.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 380, 515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047) [M::mem_pestat] mean and std.dev: (390.11, 178.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1313) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1684, 3534, 3534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7234) [M::mem_pestat] mean and std.dev: (2655.80, 881.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9084) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 340, 1539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4191) [M::mem_pestat] mean and std.dev: (483.85, 642.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5517) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 676738 sequences (100000075 bp) in 3.639 CPU sec, 3.686 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282369, 2, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 377, 569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1247) [M::mem_pestat] mean and std.dev: (336.05, 154.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (251, 385, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1058) [M::mem_pestat] mean and std.dev: (393.99, 180.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1327) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (271, 381, 656) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1426) [M::mem_pestat] mean and std.dev: (398.03, 182.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1811) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676652 sequences (100000289 bp) in 2.377 CPU sec, 2.390 real sec... [M::mem_process_seqs] Processed 677248 reads in 202.585 CPU sec, 6.415 real sec [M::mem_process_seqs] Processed 677008 reads in 139.097 CPU sec, 4.397 real sec sorting block 81, #reads = 1077981 mem = 536879362 peak = 1314324480 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 282415, 0, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 198, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (247.83, 156.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (253, 387, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060) [M::mem_pestat] mean and std.dev: (396.22, 181.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (265, 364, 541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093) [M::mem_pestat] mean and std.dev: (354.28, 139.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1369) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676544 sequences (100000021 bp) in 3.559 CPU sec, 3.643 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 282103, 0, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 354, 611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1447) [M::mem_pestat] mean and std.dev: (416.23, 257.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1865) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (255, 390, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068) [M::mem_pestat] mean and std.dev: (398.35, 181.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1339) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 373, 2080) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5834) [M::mem_pestat] mean and std.dev: (970.91, 1355.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7711) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676738 reads in 199.719 CPU sec, 6.328 real sec [M::process] read 676618 sequences (100000149 bp) in 2.572 CPU sec, 2.579 real sec... sorting block 82, #reads = 1078338 mem = 536876339 peak = 1314324480 [M::mem_process_seqs] Processed 676652 reads in 138.424 CPU sec, 4.363 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 282097, 2, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 346, 585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1367) [M::mem_pestat] mean and std.dev: (331.24, 179.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (255, 392, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1077) [M::mem_pestat] mean and std.dev: (400.71, 183.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1351) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (246, 288, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (295.32, 104.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681646 sequences (100000156 bp) in 3.669 CPU sec, 3.718 real sec... sorting block 83, #reads = 1077565 mem = 536879107 peak = 1314324480 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 281906, 1, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 234, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1185) [M::mem_pestat] mean and std.dev: (214.37, 109.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (255, 389, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068) [M::mem_pestat] mean and std.dev: (398.65, 182.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1339) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 421, 1101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2865) [M::mem_pestat] mean and std.dev: (398.67, 286.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3747) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676544 reads in 198.693 CPU sec, 6.315 real sec [M::process] read 684596 sequences (100000062 bp) in 2.806 CPU sec, 2.806 real sec... [M::mem_process_seqs] Processed 676618 reads in 138.842 CPU sec, 4.386 real sec sorting block 84, #reads = 1077571 mem = 536879161 peak = 1314324480 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 284491, 2, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (317, 393, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (350.52, 109.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 993) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (223, 354, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045) [M::mem_pestat] mean and std.dev: (370.03, 180.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1319) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 322, 582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1328) [M::mem_pestat] mean and std.dev: (367.74, 221.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1701) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682912 sequences (100000037 bp) in 3.377 CPU sec, 3.424 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 285503, 6, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 204, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (216.42, 90.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 330, 478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022) [M::mem_pestat] mean and std.dev: (352.00, 175.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1294) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 243, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (277.69, 128.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682928 sequences (100000189 bp) in 2.526 CPU sec, 2.532 real sec... [M::mem_process_seqs] Processed 681646 reads in 225.048 CPU sec, 7.143 real sec [M::mem_process_seqs] Processed 684596 reads in 158.977 CPU sec, 5.031 real sec sorting block 85, #reads = 1077125 mem = 536881080 peak = 1314324480 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 283555, 2, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 312, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (305.56, 130.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 344, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046) [M::mem_pestat] mean and std.dev: (362.59, 178.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1323) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 226, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (273.54, 131.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682666 sequences (100000018 bp) in 3.319 CPU sec, 3.407 real sec... [M::process] read 681952 sequences (100000020 bp) in 2.452 CPU sec, 2.470 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 284073, 3, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 233, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (239.12, 116.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 346, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043) [M::mem_pestat] mean and std.dev: (361.93, 176.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1319) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 392, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1087) [M::mem_pestat] mean and std.dev: (398.51, 216.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1377) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682912 reads in 231.524 CPU sec, 7.353 real sec sorting block 86, #reads = 1078206 mem = 536880374 peak = 1314324480 [M::mem_process_seqs] Processed 682928 reads in 155.166 CPU sec, 4.931 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283830, 2, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 244, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1071) [M::mem_pestat] mean and std.dev: (283.60, 151.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (217, 348, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036) [M::mem_pestat] mean and std.dev: (362.23, 174.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1309) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 236, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840) [M::mem_pestat] mean and std.dev: (251.78, 127.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1066) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681046 sequences (100000068 bp) in 3.580 CPU sec, 3.688 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 283572, 0, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 336, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (296.25, 165.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (220, 352, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027) [M::mem_pestat] mean and std.dev: (363.79, 173.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1296) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 203, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1183) [M::mem_pestat] mean and std.dev: (304.83, 179.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1519) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680594 sequences (100000186 bp) in 2.648 CPU sec, 2.666 real sec... [M::mem_process_seqs] Processed 682666 reads in 225.532 CPU sec, 7.157 real sec sorting block 87, #reads = 1078387 mem = 536880902 peak = 1314324480 [M::mem_process_seqs] Processed 681952 reads in 152.209 CPU sec, 4.820 real sec sorting block 88, #reads = 1078790 mem = 536879534 peak = 1314324480 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 282899, 3, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 250, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846) [M::mem_pestat] mean and std.dev: (220.12, 75.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1067) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (227, 361, 499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043) [M::mem_pestat] mean and std.dev: (371.78, 176.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1315) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (314, 443, 612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1208) [M::mem_pestat] mean and std.dev: (405.31, 221.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1506) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680420 sequences (100000113 bp) in 3.631 CPU sec, 3.707 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 283259, 6, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 190, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (191.73, 54.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (229, 366, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1057) [M::mem_pestat] mean and std.dev: (376.07, 178.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 281, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (297.92, 131.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680030 sequences (100000117 bp) in 2.372 CPU sec, 2.387 real sec... [M::mem_process_seqs] Processed 681046 reads in 224.979 CPU sec, 7.154 real sec [M::mem_process_seqs] Processed 680594 reads in 151.116 CPU sec, 4.789 real sec sorting block 89, #reads = 1078643 mem = 536877963 peak = 1314324480 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 282835, 2, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 431, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952) [M::mem_pestat] mean and std.dev: (349.94, 160.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1181) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (230, 366, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1049) [M::mem_pestat] mean and std.dev: (375.28, 176.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1322) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (254, 474, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1064) [M::mem_pestat] mean and std.dev: (412.64, 174.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1334) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679972 sequences (100000179 bp) in 3.596 CPU sec, 3.649 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 282634, 4, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 277, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (303.02, 114.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (232, 368, 506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054) [M::mem_pestat] mean and std.dev: (377.68, 177.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1328) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 256, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021) [M::mem_pestat] mean and std.dev: (383.50, 273.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1476) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679948 sequences (100000074 bp) in 2.520 CPU sec, 2.524 real sec... [M::mem_process_seqs] Processed 680420 reads in 224.447 CPU sec, 7.116 real sec [M::mem_process_seqs] Processed 680030 reads in 152.035 CPU sec, 4.813 real sec sorting block 90, #reads = 1077307 mem = 536877221 peak = 1314324480 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282547, 11, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 356, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1213) [M::mem_pestat] mean and std.dev: (343.02, 193.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (233, 372, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1067) [M::mem_pestat] mean and std.dev: (380.29, 178.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1345) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (533, 533, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (533, 533) [M::mem_pestat] mean and std.dev: (533.00, 0.00) [M::mem_pestat] low and high boundaries for proper pairs: (533, 533) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (334, 381, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (38, 778) [M::mem_pestat] mean and std.dev: (387.93, 124.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 679608 sequences (100000223 bp) in 3.393 CPU sec, 3.443 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282676, 4, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 320, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (312.96, 120.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (233, 369, 506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052) [M::mem_pestat] mean and std.dev: (377.22, 175.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1325) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 276, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (285.12, 91.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680016 sequences (100000030 bp) in 2.547 CPU sec, 2.548 real sec... [M::mem_process_seqs] Processed 679972 reads in 223.065 CPU sec, 7.093 real sec sorting block 91, #reads = 1077644 mem = 536882553 peak = 1314324480 [M::mem_process_seqs] Processed 679948 reads in 149.950 CPU sec, 4.744 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282224, 1, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 298, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (301.65, 102.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (236, 372, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1058) [M::mem_pestat] mean and std.dev: (380.59, 176.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1332) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (292, 386, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (342.94, 100.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1164) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679700 sequences (100000050 bp) in 3.640 CPU sec, 3.730 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282552, 2, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 202, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (96, 296) [M::mem_pestat] mean and std.dev: (191.38, 31.68) [M::mem_pestat] low and high boundaries for proper pairs: (56, 336) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (233, 370, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055) [M::mem_pestat] mean and std.dev: (377.79, 176.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 312, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046) [M::mem_pestat] mean and std.dev: (337.08, 175.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1334) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679830 sequences (100000028 bp) in 2.539 CPU sec, 2.543 real sec... [M::mem_process_seqs] Processed 679608 reads in 228.454 CPU sec, 7.247 real sec sorting block 92, #reads = 1078395 mem = 536881797 peak = 1314324480 [M::mem_process_seqs] Processed 680016 reads in 155.290 CPU sec, 4.938 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 282858, 0, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 331, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (328.08, 125.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (234, 372, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1059) [M::mem_pestat] mean and std.dev: (379.56, 176.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 219, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (255.96, 106.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1001) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 93, #reads = 1077340 mem = 536879253 peak = 1331429376 [M::process] read 679528 sequences (100000231 bp) in 3.251 CPU sec, 3.333 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 283177, 0, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 415, 1582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4264) [M::mem_pestat] mean and std.dev: (663.78, 613.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (234, 372, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056) [M::mem_pestat] mean and std.dev: (379.46, 176.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1330) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 314, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044) [M::mem_pestat] mean and std.dev: (347.31, 195.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1332) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679800 sequences (100000199 bp) in 2.561 CPU sec, 2.584 real sec... [M::mem_process_seqs] Processed 679700 reads in 232.659 CPU sec, 7.432 real sec [M::mem_process_seqs] Processed 679830 reads in 158.428 CPU sec, 5.036 real sec sorting block 94, #reads = 1077526 mem = 536876802 peak = 1331429376 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282535, 0, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 264, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (281.08, 125.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (236, 372, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052) [M::mem_pestat] mean and std.dev: (379.60, 175.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1324) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 361, 632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1422) [M::mem_pestat] mean and std.dev: (404.93, 208.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1817) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679486 sequences (100000272 bp) in 3.606 CPU sec, 3.671 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282673, 0, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 212, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1094) [M::mem_pestat] mean and std.dev: (237.07, 148.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1412) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (234, 371, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056) [M::mem_pestat] mean and std.dev: (379.20, 176.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1330) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (245, 334, 852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2066) [M::mem_pestat] mean and std.dev: (462.93, 325.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2673) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679208 sequences (100000216 bp) in 2.537 CPU sec, 2.540 real sec... [M::mem_process_seqs] Processed 679528 reads in 234.799 CPU sec, 7.464 real sec [M::mem_process_seqs] Processed 679800 reads in 159.320 CPU sec, 5.051 real sec sorting block 95, #reads = 1075370 mem = 536878861 peak = 1331429376 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282169, 0, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (295, 454, 592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1186) [M::mem_pestat] mean and std.dev: (387.12, 192.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (236, 373, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1058) [M::mem_pestat] mean and std.dev: (380.94, 176.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1332) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 358, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027) [M::mem_pestat] mean and std.dev: (354.64, 167.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1299) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679384 sequences (100000119 bp) in 3.626 CPU sec, 3.720 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 282430, 2, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 256, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017) [M::mem_pestat] mean and std.dev: (289.33, 130.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1288) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (237, 376, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068) [M::mem_pestat] mean and std.dev: (383.60, 178.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1345) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 354, 542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1152) [M::mem_pestat] mean and std.dev: (359.42, 147.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1457) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678982 sequences (100000026 bp) in 2.600 CPU sec, 2.602 real sec... [M::mem_process_seqs] Processed 679486 reads in 234.846 CPU sec, 7.460 real sec sorting block 96, #reads = 1076155 mem = 536880207 peak = 1331429376 [M::mem_process_seqs] Processed 679208 reads in 160.826 CPU sec, 5.088 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 282700, 0, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (276, 306, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 501) [M::mem_pestat] mean and std.dev: (299.70, 77.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (237, 374, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056) [M::mem_pestat] mean and std.dev: (381.27, 175.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (305, 435, 596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1178) [M::mem_pestat] mean and std.dev: (409.86, 158.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1469) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678902 sequences (100000118 bp) in 3.633 CPU sec, 3.695 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282773, 1, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 280, 5053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14693) [M::mem_pestat] mean and std.dev: (1711.15, 2130.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (239, 374, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1049) [M::mem_pestat] mean and std.dev: (381.45, 175.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1319) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 311, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024) [M::mem_pestat] mean and std.dev: (323.66, 146.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1306) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678746 sequences (100000208 bp) in 2.540 CPU sec, 2.542 real sec... [M::mem_process_seqs] Processed 679384 reads in 239.921 CPU sec, 7.623 real sec sorting block 97, #reads = 1076505 mem = 536879162 peak = 1331429376 [M::mem_process_seqs] Processed 678982 reads in 163.508 CPU sec, 5.184 real sec sorting block 98, #reads = 1076392 mem = 536880018 peak = 1331752960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 282063, 1, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 298, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (261.87, 90.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (239, 373, 506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040) [M::mem_pestat] mean and std.dev: (380.01, 173.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1307) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (310, 336, 653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1339) [M::mem_pestat] mean and std.dev: (454.10, 234.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1682) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678318 sequences (100000136 bp) in 3.604 CPU sec, 3.662 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 282216, 4, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 288, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (262.46, 97.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (239, 372, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037) [M::mem_pestat] mean and std.dev: (378.95, 172.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1303) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 324, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971) [M::mem_pestat] mean and std.dev: (301.88, 121.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1222) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678108 sequences (100000107 bp) in 2.550 CPU sec, 2.563 real sec... [M::mem_process_seqs] Processed 678902 reads in 241.039 CPU sec, 7.659 real sec [M::mem_process_seqs] Processed 678746 reads in 165.602 CPU sec, 5.255 real sec sorting block 99, #reads = 1074768 mem = 536880424 peak = 1331752960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 281874, 1, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 312, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (319.24, 141.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1065) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (241, 372, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027) [M::mem_pestat] mean and std.dev: (378.91, 171.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1289) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (294, 350, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (44, 669) [M::mem_pestat] mean and std.dev: (349.33, 109.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678012 sequences (100000200 bp) in 3.637 CPU sec, 3.675 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 282212, 7, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 322, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (330.35, 186.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 375, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036) [M::mem_pestat] mean and std.dev: (382.48, 173.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1300) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 331, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (287.70, 127.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677802 sequences (100000073 bp) in 2.517 CPU sec, 2.522 real sec... [M::mem_process_seqs] Processed 678318 reads in 234.548 CPU sec, 7.443 real sec [M::mem_process_seqs] Processed 678108 reads in 158.994 CPU sec, 5.039 real sec sorting block 100, #reads = 1074102 mem = 536877545 peak = 1331752960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 282064, 1, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 279, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1179) [M::mem_pestat] mean and std.dev: (303.57, 166.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 377, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045) [M::mem_pestat] mean and std.dev: (384.10, 174.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1312) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (250, 384, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (347.20, 133.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678020 sequences (100000230 bp) in 3.600 CPU sec, 3.659 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 282586, 2, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (275, 347, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848) [M::mem_pestat] mean and std.dev: (352.51, 144.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 382, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046) [M::mem_pestat] mean and std.dev: (387.89, 174.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1312) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 286, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (304.64, 146.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1176) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677794 sequences (100000249 bp) in 2.543 CPU sec, 2.546 real sec... [M::mem_process_seqs] Processed 678012 reads in 232.935 CPU sec, 7.396 real sec sorting block 101, #reads = 1075487 mem = 536881675 peak = 1331752960 [M::mem_process_seqs] Processed 677802 reads in 155.542 CPU sec, 4.920 real sec sorting block 102, #reads = 1074385 mem = 536880906 peak = 1331752960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 282318, 2, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 253, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (264.68, 94.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 376, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036) [M::mem_pestat] mean and std.dev: (383.24, 173.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1300) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (244, 370, 603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1321) [M::mem_pestat] mean and std.dev: (396.68, 215.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1680) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677710 sequences (100000106 bp) in 3.622 CPU sec, 3.667 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 282859, 4, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 255, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (246.00, 114.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 384, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1061) [M::mem_pestat] mean and std.dev: (391.14, 178.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1332) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 341, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (320.12, 128.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681766 sequences (100000070 bp) in 2.590 CPU sec, 2.587 real sec... [M::mem_process_seqs] Processed 678020 reads in 226.785 CPU sec, 7.185 real sec [M::mem_process_seqs] Processed 677794 reads in 156.171 CPU sec, 4.932 real sec sorting block 103, #reads = 1074102 mem = 536880184 peak = 1331752960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 282715, 2, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 255, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1057) [M::mem_pestat] mean and std.dev: (261.89, 142.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1358) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 381, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1059) [M::mem_pestat] mean and std.dev: (389.22, 178.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1330) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 306, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (316.58, 120.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 690470 sequences (100000202 bp) in 3.468 CPU sec, 3.504 real sec... [M::process] read 688616 sequences (100000222 bp) in 2.671 CPU sec, 2.678 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 284092, 6, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 332, 654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1598) [M::mem_pestat] mean and std.dev: (352.52, 214.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2070) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (220, 351, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039) [M::mem_pestat] mean and std.dev: (366.35, 177.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1312) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 298, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (289.10, 108.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677710 reads in 233.843 CPU sec, 7.403 real sec [M::mem_process_seqs] Processed 681766 reads in 167.464 CPU sec, 5.310 real sec sorting block 104, #reads = 1073982 mem = 536881541 peak = 1331752960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 287397, 5, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 304, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1064) [M::mem_pestat] mean and std.dev: (357.12, 222.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1351) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 289, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (312.18, 155.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 291, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (276.68, 111.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687544 sequences (100000171 bp) in 3.376 CPU sec, 3.450 real sec... [M::process] read 686556 sequences (100000033 bp) in 2.575 CPU sec, 2.589 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 286956, 5, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 254, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1252) [M::mem_pestat] mean and std.dev: (271.73, 183.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 305, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (325.21, 161.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1195) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 290, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (328.26, 162.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690470 reads in 264.856 CPU sec, 8.429 real sec sorting block 105, #reads = 1072413 mem = 536879577 peak = 1331752960 [M::mem_process_seqs] Processed 688616 reads in 181.308 CPU sec, 5.765 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 286577, 4, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 230, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (230.68, 103.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 313, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960) [M::mem_pestat] mean and std.dev: (331.25, 162.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1215) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (139, 297, 801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2125) [M::mem_pestat] mean and std.dev: (475.68, 542.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2787) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685174 sequences (100000292 bp) in 3.425 CPU sec, 3.478 real sec... [M::process] read 684040 sequences (100000009 bp) in 2.659 CPU sec, 2.664 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 286520, 1, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 267, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (248.51, 119.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 321, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974) [M::mem_pestat] mean and std.dev: (337.77, 165.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1232) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 235, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (244.76, 104.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687544 reads in 251.512 CPU sec, 7.992 real sec sorting block 106, #reads = 1073608 mem = 536885016 peak = 1331752960 [M::mem_process_seqs] Processed 686556 reads in 172.186 CPU sec, 5.468 real sec sorting block 107, #reads = 1073288 mem = 536879930 peak = 1335562240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 285931, 2, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 240, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997) [M::mem_pestat] mean and std.dev: (278.24, 137.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1275) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 329, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982) [M::mem_pestat] mean and std.dev: (342.88, 165.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1241) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 195, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004) [M::mem_pestat] mean and std.dev: (252.33, 185.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1289) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683428 sequences (100000101 bp) in 3.712 CPU sec, 3.772 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 285202, 9, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (239, 254, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (61, 506) [M::mem_pestat] mean and std.dev: (273.73, 99.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 333, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980) [M::mem_pestat] mean and std.dev: (345.43, 163.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1237) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 363, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (321.59, 132.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1173) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682744 sequences (100000260 bp) in 2.639 CPU sec, 2.650 real sec... [M::mem_process_seqs] Processed 685174 reads in 244.828 CPU sec, 7.778 real sec [M::mem_process_seqs] Processed 684040 reads in 168.766 CPU sec, 5.353 real sec sorting block 108, #reads = 1074202 mem = 536880116 peak = 1335562240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 284987, 1, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 227, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (217.72, 93.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 338, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995) [M::mem_pestat] mean and std.dev: (350.22, 166.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1256) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 341, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1153) [M::mem_pestat] mean and std.dev: (322.84, 139.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1461) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682538 sequences (100000231 bp) in 3.627 CPU sec, 3.671 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 284496, 4, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 255, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (237.20, 80.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 344, 478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002) [M::mem_pestat] mean and std.dev: (355.11, 167.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1264) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 232, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (43, 413) [M::mem_pestat] mean and std.dev: (219.56, 51.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681980 sequences (100000038 bp) in 2.543 CPU sec, 2.558 real sec... [M::mem_process_seqs] Processed 683428 reads in 240.596 CPU sec, 7.641 real sec [M::mem_process_seqs] Processed 682744 reads in 164.664 CPU sec, 5.226 real sec sorting block 109, #reads = 1073699 mem = 536877984 peak = 1335562240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 284517, 2, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 216, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (236.46, 104.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (217, 345, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003) [M::mem_pestat] mean and std.dev: (356.27, 167.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1265) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (130, 203, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (236.80, 124.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681182 sequences (100000129 bp) in 3.710 CPU sec, 3.779 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 284693, 1, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 289, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (75, 435) [M::mem_pestat] mean and std.dev: (233.11, 64.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (220, 349, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006) [M::mem_pestat] mean and std.dev: (359.21, 168.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1268) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 312, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (303.18, 116.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681192 sequences (100000205 bp) in 2.662 CPU sec, 2.668 real sec... [M::mem_process_seqs] Processed 682538 reads in 237.251 CPU sec, 7.520 real sec sorting block 110, #reads = 1073559 mem = 536878274 peak = 1335562240 [M::mem_process_seqs] Processed 681980 reads in 163.201 CPU sec, 5.161 real sec sorting block 111, #reads = 1073675 mem = 536882781 peak = 1335562240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 283455, 0, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 312, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1166) [M::mem_pestat] mean and std.dev: (348.80, 210.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (224, 356, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025) [M::mem_pestat] mean and std.dev: (365.80, 171.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1292) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (137, 290, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947) [M::mem_pestat] mean and std.dev: (274.24, 130.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1217) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680716 sequences (100000242 bp) in 3.507 CPU sec, 3.552 real sec... [M::process] read 681178 sequences (100000079 bp) in 2.610 CPU sec, 2.612 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283994, 1, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 327, 598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1480) [M::mem_pestat] mean and std.dev: (293.25, 161.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1921) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (224, 356, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022) [M::mem_pestat] mean and std.dev: (365.53, 171.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1288) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 306, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1110) [M::mem_pestat] mean and std.dev: (322.29, 175.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1411) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681182 reads in 233.002 CPU sec, 7.382 real sec [M::mem_process_seqs] Processed 681192 reads in 160.166 CPU sec, 5.081 real sec sorting block 112, #reads = 1074390 mem = 536881494 peak = 1335562240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 283920, 7, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 224, 604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1462) [M::mem_pestat] mean and std.dev: (380.97, 353.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1891) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (227, 358, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025) [M::mem_pestat] mean and std.dev: (367.80, 171.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1291) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (247, 286, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (275.43, 89.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680548 sequences (100000123 bp) in 3.637 CPU sec, 3.668 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 283582, 7, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 295, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (250.11, 99.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (223, 354, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018) [M::mem_pestat] mean and std.dev: (364.02, 170.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1283) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 264, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (290.68, 128.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679850 sequences (100000169 bp) in 2.557 CPU sec, 2.562 real sec... [M::mem_process_seqs] Processed 680716 reads in 233.833 CPU sec, 7.424 real sec [M::mem_process_seqs] Processed 681178 reads in 162.230 CPU sec, 5.131 real sec sorting block 113, #reads = 1075185 mem = 536881847 peak = 1335562240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283305, 4, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 251, 546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1230) [M::mem_pestat] mean and std.dev: (344.27, 243.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (228, 361, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038) [M::mem_pestat] mean and std.dev: (370.98, 174.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1308) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 343, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (319.93, 132.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679392 sequences (100000053 bp) in 3.377 CPU sec, 3.423 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 283146, 1, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 265, 598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1448) [M::mem_pestat] mean and std.dev: (340.74, 275.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1873) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (233, 370, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1058) [M::mem_pestat] mean and std.dev: (378.83, 177.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 350, 1018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2650) [M::mem_pestat] mean and std.dev: (329.58, 209.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3466) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678904 sequences (100000122 bp) in 2.568 CPU sec, 2.571 real sec... [M::mem_process_seqs] Processed 680548 reads in 233.250 CPU sec, 7.418 real sec [M::mem_process_seqs] Processed 679850 reads in 160.556 CPU sec, 5.087 real sec sorting block 114, #reads = 1075172 mem = 536879169 peak = 1335562240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282840, 2, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 237, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1194) [M::mem_pestat] mean and std.dev: (303.58, 141.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (235, 377, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1087) [M::mem_pestat] mean and std.dev: (385.67, 181.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1371) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 276, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039) [M::mem_pestat] mean and std.dev: (311.97, 193.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1314) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678544 sequences (100000115 bp) in 3.724 CPU sec, 3.776 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 283160, 5, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 342, 687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1625) [M::mem_pestat] mean and std.dev: (419.51, 365.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2094) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (239, 380, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1091) [M::mem_pestat] mean and std.dev: (389.46, 182.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1375) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 335, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (302.60, 111.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1068) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678810 sequences (100000045 bp) in 2.570 CPU sec, 2.566 real sec... [M::mem_process_seqs] Processed 679392 reads in 235.332 CPU sec, 7.468 real sec sorting block 115, #reads = 1075001 mem = 536883025 peak = 1335562240 [M::mem_process_seqs] Processed 678904 reads in 161.376 CPU sec, 5.110 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 282613, 2, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 367, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (304.89, 118.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (240, 381, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1083) [M::mem_pestat] mean and std.dev: (389.08, 181.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1364) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 266, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1077) [M::mem_pestat] mean and std.dev: (321.23, 168.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1367) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 116, #reads = 1075130 mem = 536886827 peak = 1335562240 [M::process] read 678218 sequences (100000186 bp) in 3.373 CPU sec, 3.415 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 283181, 2, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 325, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (287.74, 122.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (238, 376, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075) [M::mem_pestat] mean and std.dev: (386.18, 180.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1354) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (263, 419, 625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1349) [M::mem_pestat] mean and std.dev: (398.84, 178.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1711) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678158 sequences (100000032 bp) in 2.573 CPU sec, 2.584 real sec... [M::mem_process_seqs] Processed 678544 reads in 237.534 CPU sec, 7.557 real sec [M::mem_process_seqs] Processed 678810 reads in 163.741 CPU sec, 5.190 real sec sorting block 117, #reads = 1073553 mem = 536885387 peak = 1335562240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 282477, 2, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 249, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (266.42, 135.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (242, 381, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1082) [M::mem_pestat] mean and std.dev: (390.37, 181.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1362) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (265, 428, 575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1195) [M::mem_pestat] mean and std.dev: (367.76, 157.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1505) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677752 sequences (100000191 bp) in 3.666 CPU sec, 3.704 real sec... [M::process] read 677714 sequences (100000235 bp) in 1.980 CPU sec, 1.985 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 282170, 2, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (222, 277, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996) [M::mem_pestat] mean and std.dev: (327.97, 186.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1254) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 382, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1081) [M::mem_pestat] mean and std.dev: (391.79, 181.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1360) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (264, 341, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975) [M::mem_pestat] mean and std.dev: (348.22, 139.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1212) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 678218 reads in 228.299 CPU sec, 7.265 real sec [M::mem_process_seqs] Processed 678158 reads in 158.115 CPU sec, 5.018 real sec sorting block 118, #reads = 1076063 mem = 536878711 peak = 1335562240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282905, 3, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 270, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1170) [M::mem_pestat] mean and std.dev: (338.67, 207.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 387, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1090) [M::mem_pestat] mean and std.dev: (396.47, 184.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1371) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 337, 579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1351) [M::mem_pestat] mean and std.dev: (351.22, 191.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1737) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677702 sequences (100000248 bp) in 3.673 CPU sec, 3.715 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282639, 9, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 224, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (268.28, 96.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 389, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1105) [M::mem_pestat] mean and std.dev: (398.62, 186.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1391) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 246, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (273.19, 98.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677366 sequences (100000299 bp) in 2.568 CPU sec, 2.574 real sec... [M::mem_process_seqs] Processed 677752 reads in 226.793 CPU sec, 7.191 real sec sorting block 119, #reads = 1075127 mem = 536882008 peak = 1335562240 [M::mem_process_seqs] Processed 677714 reads in 157.300 CPU sec, 4.984 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282720, 1, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 270, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1147) [M::mem_pestat] mean and std.dev: (321.21, 250.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 388, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1106) [M::mem_pestat] mean and std.dev: (399.54, 187.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1392) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 392, 780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1874) [M::mem_pestat] mean and std.dev: (351.21, 173.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2421) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677544 sequences (100000099 bp) in 3.628 CPU sec, 3.684 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 282422, 1, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (274, 397, 572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1168) [M::mem_pestat] mean and std.dev: (398.76, 172.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (251, 393, 540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1118) [M::mem_pestat] mean and std.dev: (404.34, 190.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1407) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 357, 579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1317) [M::mem_pestat] mean and std.dev: (350.94, 246.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1686) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684394 sequences (100000193 bp) in 2.492 CPU sec, 2.494 real sec... [M::mem_process_seqs] Processed 677702 reads in 228.692 CPU sec, 7.263 real sec sorting block 120, #reads = 1074817 mem = 536880937 peak = 1335562240 [M::mem_process_seqs] Processed 677366 reads in 161.300 CPU sec, 5.112 real sec sorting block 121, #reads = 1074425 mem = 536881211 peak = 1335562240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282290, 0, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 273, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (267.25, 108.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 392, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1115) [M::mem_pestat] mean and std.dev: (402.89, 191.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1404) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 297, 555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1223) [M::mem_pestat] mean and std.dev: (347.67, 163.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1557) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683780 sequences (100000033 bp) in 3.701 CPU sec, 3.752 real sec... [M::process] read 682108 sequences (100000295 bp) in 2.576 CPU sec, 2.583 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 284900, 0, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 262, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960) [M::mem_pestat] mean and std.dev: (302.31, 148.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1212) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 331, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011) [M::mem_pestat] mean and std.dev: (351.60, 175.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1279) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 322, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023) [M::mem_pestat] mean and std.dev: (335.33, 143.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1286) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677544 reads in 253.790 CPU sec, 8.071 real sec [M::mem_process_seqs] Processed 684394 reads in 180.144 CPU sec, 5.719 real sec sorting block 122, #reads = 1067835 mem = 536883320 peak = 1335562240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 284693, 3, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (315, 380, 609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1197) [M::mem_pestat] mean and std.dev: (372.70, 160.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 338, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021) [M::mem_pestat] mean and std.dev: (356.46, 176.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1291) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 365, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1217) [M::mem_pestat] mean and std.dev: (387.13, 272.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1563) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681454 sequences (100000201 bp) in 3.376 CPU sec, 3.414 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 284032, 3, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 225, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989) [M::mem_pestat] mean and std.dev: (275.32, 161.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1266) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (220, 350, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030) [M::mem_pestat] mean and std.dev: (364.88, 176.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1300) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 324, 622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1506) [M::mem_pestat] mean and std.dev: (338.09, 231.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1948) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681148 sequences (100000229 bp) in 2.522 CPU sec, 2.528 real sec... [M::mem_process_seqs] Processed 683780 reads in 222.024 CPU sec, 7.041 real sec [M::mem_process_seqs] Processed 682108 reads in 148.875 CPU sec, 4.705 real sec sorting block 123, #reads = 1076830 mem = 536877878 peak = 1335562240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283979, 1, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 368, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1351) [M::mem_pestat] mean and std.dev: (394.04, 250.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (223, 354, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033) [M::mem_pestat] mean and std.dev: (368.06, 176.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1303) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 268, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (262.09, 116.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680834 sequences (100000273 bp) in 3.691 CPU sec, 3.749 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283702, 0, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 315, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (272.05, 96.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (224, 356, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037) [M::mem_pestat] mean and std.dev: (368.85, 176.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1308) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 289, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (324.88, 185.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680326 sequences (100000113 bp) in 2.545 CPU sec, 2.548 real sec... [M::mem_process_seqs] Processed 681454 reads in 216.768 CPU sec, 6.869 real sec sorting block 124, #reads = 1079858 mem = 536885688 peak = 1335562240 [M::mem_process_seqs] Processed 681148 reads in 148.888 CPU sec, 4.710 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 283529, 3, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 308, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (270.84, 134.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (228, 361, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047) [M::mem_pestat] mean and std.dev: (374.13, 179.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1320) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 297, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (294.51, 120.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1090) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 125, #reads = 1079072 mem = 536878440 peak = 1335562240 [M::process] read 680152 sequences (100000251 bp) in 3.514 CPU sec, 3.589 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283370, 0, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 277, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (294.66, 159.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (229, 361, 499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039) [M::mem_pestat] mean and std.dev: (373.29, 177.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1309) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 303, 573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1363) [M::mem_pestat] mean and std.dev: (328.48, 193.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1758) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680362 sequences (100000156 bp) in 2.460 CPU sec, 2.471 real sec... [M::mem_process_seqs] Processed 680834 reads in 214.581 CPU sec, 6.820 real sec [M::mem_process_seqs] Processed 680326 reads in 146.733 CPU sec, 4.646 real sec sorting block 126, #reads = 1079613 mem = 536879258 peak = 1335562240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 283526, 2, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 287, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (299.80, 114.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (231, 363, 499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035) [M::mem_pestat] mean and std.dev: (374.16, 176.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1303) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 257, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (13, 508) [M::mem_pestat] mean and std.dev: (241.71, 68.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679600 sequences (100000000 bp) in 3.300 CPU sec, 3.339 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283407, 4, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 407, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1199) [M::mem_pestat] mean and std.dev: (412.10, 232.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (230, 362, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034) [M::mem_pestat] mean and std.dev: (373.08, 175.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1302) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 259, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (278.59, 77.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679310 sequences (100000248 bp) in 2.483 CPU sec, 2.493 real sec... [M::mem_process_seqs] Processed 680152 reads in 216.840 CPU sec, 6.894 real sec [M::mem_process_seqs] Processed 680362 reads in 148.292 CPU sec, 4.693 real sec sorting block 127, #reads = 1077957 mem = 536878502 peak = 1335562240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 283743, 7, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 189, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1227) [M::mem_pestat] mean and std.dev: (333.31, 275.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (235, 369, 506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048) [M::mem_pestat] mean and std.dev: (379.96, 179.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1319) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 321, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (310.77, 102.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679244 sequences (100000172 bp) in 3.246 CPU sec, 3.321 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 282788, 0, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 252, 1114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2972) [M::mem_pestat] mean and std.dev: (506.45, 686.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3901) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (237, 370, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047) [M::mem_pestat] mean and std.dev: (380.87, 178.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1317) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (345, 456, 632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1206) [M::mem_pestat] mean and std.dev: (448.93, 185.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1493) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 679600 reads in 213.758 CPU sec, 6.797 real sec [M::process] read 678940 sequences (100000080 bp) in 2.529 CPU sec, 2.534 real sec... [M::mem_process_seqs] Processed 679310 reads in 146.362 CPU sec, 4.622 real sec sorting block 128, #reads = 1078573 mem = 536877601 peak = 1335562240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283320, 1, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 229, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993) [M::mem_pestat] mean and std.dev: (253.26, 155.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1270) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (238, 373, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051) [M::mem_pestat] mean and std.dev: (382.37, 178.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1322) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 284, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948) [M::mem_pestat] mean and std.dev: (311.78, 160.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1204) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679492 sequences (100000220 bp) in 3.200 CPU sec, 3.242 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 283301, 1, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 216, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (272.23, 144.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (239, 377, 515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1067) [M::mem_pestat] mean and std.dev: (385.76, 180.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1343) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 244, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (267.37, 130.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680398 sequences (100000102 bp) in 2.445 CPU sec, 2.445 real sec... [M::mem_process_seqs] Processed 679244 reads in 211.258 CPU sec, 6.702 real sec sorting block 129, #reads = 1078425 mem = 536879813 peak = 1335562240 [M::mem_process_seqs] Processed 678940 reads in 144.241 CPU sec, 4.576 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 283247, 2, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 219, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038) [M::mem_pestat] mean and std.dev: (281.86, 159.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1331) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (234, 369, 506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050) [M::mem_pestat] mean and std.dev: (379.27, 178.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1322) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 302, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (318.46, 151.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1093) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 130, #reads = 1079634 mem = 536879010 peak = 1335562240 [M::process] read 679268 sequences (100000082 bp) in 3.331 CPU sec, 3.381 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 283170, 2, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 187, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1285) [M::mem_pestat] mean and std.dev: (376.55, 392.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1948) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (228, 360, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038) [M::mem_pestat] mean and std.dev: (372.32, 177.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1308) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (260, 455, 761) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1763) [M::mem_pestat] mean and std.dev: (408.16, 213.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2264) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 679492 reads in 217.534 CPU sec, 6.927 real sec [M::process] read 679492 sequences (100000205 bp) in 2.556 CPU sec, 2.559 real sec... [M::mem_process_seqs] Processed 680398 reads in 150.338 CPU sec, 4.750 real sec sorting block 131, #reads = 1078579 mem = 536882152 peak = 1335562240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 283020, 3, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 264, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (222.03, 101.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (235, 371, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054) [M::mem_pestat] mean and std.dev: (380.98, 178.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1327) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 319, 1539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4175) [M::mem_pestat] mean and std.dev: (886.65, 948.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5493) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679132 sequences (100000111 bp) in 3.575 CPU sec, 3.601 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283336, 2, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 206, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (227.85, 66.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (234, 371, 512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068) [M::mem_pestat] mean and std.dev: (382.20, 180.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1346) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 375, 594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1300) [M::mem_pestat] mean and std.dev: (367.16, 186.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1653) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678480 sequences (100000166 bp) in 2.579 CPU sec, 2.581 real sec... [M::mem_process_seqs] Processed 679268 reads in 222.179 CPU sec, 7.045 real sec [M::mem_process_seqs] Processed 679492 reads in 152.542 CPU sec, 4.832 real sec sorting block 132, #reads = 1076811 mem = 536878637 peak = 1335562240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 282896, 7, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 256, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (264.00, 119.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (238, 375, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066) [M::mem_pestat] mean and std.dev: (384.63, 180.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1342) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 269, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (283.94, 131.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678146 sequences (100000082 bp) in 3.334 CPU sec, 3.382 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 282418, 5, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 252, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1180) [M::mem_pestat] mean and std.dev: (327.67, 188.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (240, 377, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068) [M::mem_pestat] mean and std.dev: (386.99, 180.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1344) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 295, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1065) [M::mem_pestat] mean and std.dev: (321.09, 144.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1343) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678224 sequences (100000233 bp) in 2.496 CPU sec, 2.502 real sec... [M::mem_process_seqs] Processed 679132 reads in 223.370 CPU sec, 7.103 real sec [M::mem_process_seqs] Processed 678480 reads in 152.270 CPU sec, 4.817 real sec sorting block 133, #reads = 1076863 mem = 536883769 peak = 1335562240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281700, 0, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 297, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050) [M::mem_pestat] mean and std.dev: (414.52, 310.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 384, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1086) [M::mem_pestat] mean and std.dev: (392.57, 182.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1367) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 271, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 519) [M::mem_pestat] mean and std.dev: (273.05, 78.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678202 sequences (100000273 bp) in 3.315 CPU sec, 3.383 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282424, 2, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 309, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (313.84, 150.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 384, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1084) [M::mem_pestat] mean and std.dev: (392.55, 182.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1364) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (260, 461, 600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1280) [M::mem_pestat] mean and std.dev: (416.36, 226.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1620) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677916 sequences (100000280 bp) in 2.595 CPU sec, 2.604 real sec... [M::mem_process_seqs] Processed 678146 reads in 224.462 CPU sec, 7.134 real sec sorting block 134, #reads = 1076547 mem = 536880093 peak = 1335562240 [M::mem_process_seqs] Processed 678224 reads in 153.908 CPU sec, 4.874 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 282303, 4, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 253, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (259.32, 111.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 383, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1082) [M::mem_pestat] mean and std.dev: (392.32, 182.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1361) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 291, 584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1314) [M::mem_pestat] mean and std.dev: (353.29, 213.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1679) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 135, #reads = 1076718 mem = 536879673 peak = 1340690432 [M::process] read 677888 sequences (100000220 bp) in 3.318 CPU sec, 3.380 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282828, 1, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 244, 742) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1928) [M::mem_pestat] mean and std.dev: (303.44, 239.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 385, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1091) [M::mem_pestat] mean and std.dev: (394.12, 183.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1373) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 408, 609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1359) [M::mem_pestat] mean and std.dev: (355.64, 156.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1734) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678836 sequences (100000299 bp) in 2.513 CPU sec, 2.516 real sec... [M::mem_process_seqs] Processed 678202 reads in 227.849 CPU sec, 7.252 real sec [M::mem_process_seqs] Processed 677916 reads in 157.843 CPU sec, 5.005 real sec sorting block 136, #reads = 1075818 mem = 536881192 peak = 1340690432 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 282696, 4, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 280, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1273) [M::mem_pestat] mean and std.dev: (299.13, 166.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 385, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1094) [M::mem_pestat] mean and std.dev: (394.68, 184.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1377) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 391, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995) [M::mem_pestat] mean and std.dev: (377.18, 171.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1262) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677632 sequences (100000245 bp) in 3.608 CPU sec, 3.656 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 282839, 3, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 307, 604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1498) [M::mem_pestat] mean and std.dev: (345.71, 224.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1945) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (236, 373, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1079) [M::mem_pestat] mean and std.dev: (386.03, 183.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1360) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 359, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1185) [M::mem_pestat] mean and std.dev: (326.57, 166.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1515) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677922 sequences (100000100 bp) in 2.212 CPU sec, 2.224 real sec... [M::mem_process_seqs] Processed 677888 reads in 224.645 CPU sec, 7.140 real sec [M::mem_process_seqs] Processed 678836 reads in 155.984 CPU sec, 4.948 real sec sorting block 137, #reads = 1075799 mem = 536878909 peak = 1340690432 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 282660, 9, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 376, 907) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2345) [M::mem_pestat] mean and std.dev: (583.16, 506.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3064) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 383, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1085) [M::mem_pestat] mean and std.dev: (393.23, 182.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1365) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 253, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (291.05, 125.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1073) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677826 sequences (100000074 bp) in 3.128 CPU sec, 3.207 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 283175, 1, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 327, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (291.60, 132.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 380, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1072) [M::mem_pestat] mean and std.dev: (390.35, 180.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1348) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 252, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (280.47, 125.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1005) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677798 sequences (100000080 bp) in 2.602 CPU sec, 2.607 real sec... [M::mem_process_seqs] Processed 677632 reads in 223.937 CPU sec, 7.097 real sec sorting block 138, #reads = 1075380 mem = 536879285 peak = 1340690432 [M::mem_process_seqs] Processed 677922 reads in 154.602 CPU sec, 4.904 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282610, 3, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 226, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (239.04, 100.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 384, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088) [M::mem_pestat] mean and std.dev: (394.12, 184.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1369) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (257, 341, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797) [M::mem_pestat] mean and std.dev: (316.14, 129.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677920 sequences (100000072 bp) in 3.620 CPU sec, 3.664 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 282668, 0, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 378, 661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1565) [M::mem_pestat] mean and std.dev: (471.26, 303.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2017) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 383, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088) [M::mem_pestat] mean and std.dev: (393.84, 184.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1369) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 266, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (252.68, 120.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677738 sequences (100000173 bp) in 2.623 CPU sec, 2.633 real sec... [M::mem_process_seqs] Processed 677826 reads in 230.314 CPU sec, 7.310 real sec sorting block 139, #reads = 1075535 mem = 536877050 peak = 1340690432 [M::mem_process_seqs] Processed 677798 reads in 160.645 CPU sec, 5.089 real sec sorting block 140, #reads = 1075018 mem = 536878573 peak = 1341534208 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283051, 3, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 247, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (226.04, 80.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 384, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1091) [M::mem_pestat] mean and std.dev: (395.44, 186.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1373) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (268, 454, 540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1084) [M::mem_pestat] mean and std.dev: (413.76, 173.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1356) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678738 sequences (100000169 bp) in 3.668 CPU sec, 3.741 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283103, 5, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 220, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (279.46, 196.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 386, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1101) [M::mem_pestat] mean and std.dev: (398.29, 188.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1386) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 345, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (302.71, 119.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 691164 sequences (100000050 bp) in 2.719 CPU sec, 2.715 real sec... [M::mem_process_seqs] Processed 677920 reads in 234.038 CPU sec, 7.434 real sec [M::mem_process_seqs] Processed 677738 reads in 164.550 CPU sec, 5.211 real sec sorting block 141, #reads = 1073147 mem = 536879112 peak = 1341534208 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283355, 1, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 282, 563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1347) [M::mem_pestat] mean and std.dev: (400.93, 355.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1824) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (240, 378, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1089) [M::mem_pestat] mean and std.dev: (390.97, 185.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1372) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 305, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (302.67, 112.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687782 sequences (100000085 bp) in 3.543 CPU sec, 3.593 real sec... [M::process] read 685376 sequences (100000274 bp) in 2.678 CPU sec, 2.684 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 288146, 1, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 240, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (278.68, 178.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1188) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 287, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (313.44, 159.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1151) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 215, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (257.55, 134.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 678738 reads in 263.714 CPU sec, 8.394 real sec [M::mem_process_seqs] Processed 691164 reads in 186.284 CPU sec, 5.917 real sec sorting block 142, #reads = 1068731 mem = 536879212 peak = 1341534208 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 286399, 2, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (272, 360, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (319.43, 114.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 313, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978) [M::mem_pestat] mean and std.dev: (334.36, 167.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1239) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 232, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004) [M::mem_pestat] mean and std.dev: (292.96, 168.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1282) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684174 sequences (100000158 bp) in 3.702 CPU sec, 3.760 real sec... [M::process] read 683516 sequences (100000041 bp) in 2.657 CPU sec, 2.661 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 286201, 3, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 243, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (251.09, 114.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 326, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989) [M::mem_pestat] mean and std.dev: (342.69, 166.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1251) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 354, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965) [M::mem_pestat] mean and std.dev: (309.52, 147.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1214) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687782 reads in 250.736 CPU sec, 7.961 real sec sorting block 143, #reads = 1071909 mem = 536877444 peak = 1341534208 [M::mem_process_seqs] Processed 685376 reads in 172.295 CPU sec, 5.475 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 285325, 12, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 241, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1097) [M::mem_pestat] mean and std.dev: (297.79, 210.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 336, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004) [M::mem_pestat] mean and std.dev: (350.24, 168.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1269) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (706, 706, 2199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5185) [M::mem_pestat] mean and std.dev: (1265.17, 935.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6678) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 213, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (245.14, 135.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 144, #reads = 1073273 mem = 536877427 peak = 1343528960 [M::process] read 681914 sequences (100000111 bp) in 3.657 CPU sec, 3.688 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 285302, 3, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 327, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000) [M::mem_pestat] mean and std.dev: (347.92, 186.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1266) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 341, 478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010) [M::mem_pestat] mean and std.dev: (354.18, 170.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1276) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 355, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797) [M::mem_pestat] mean and std.dev: (280.62, 138.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681708 sequences (100000042 bp) in 2.690 CPU sec, 2.702 real sec... [M::mem_process_seqs] Processed 684174 reads in 246.722 CPU sec, 7.845 real sec [M::mem_process_seqs] Processed 683516 reads in 171.560 CPU sec, 5.443 real sec sorting block 145, #reads = 1073095 mem = 536881034 peak = 1343528960 [M::process] read 681172 sequences (100000276 bp) in 3.719 CPU sec, 3.765 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 284421, 8, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 356, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974) [M::mem_pestat] mean and std.dev: (334.41, 165.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1221) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (221, 353, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022) [M::mem_pestat] mean and std.dev: (363.30, 171.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1289) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 258, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1204) [M::mem_pestat] mean and std.dev: (332.04, 257.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1539) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680622 sequences (100000050 bp) in 2.641 CPU sec, 2.649 real sec... [M::mem_process_seqs] Processed 681708 reads in 139.991 CPU sec, 4.416 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 284733, 2, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (262, 291, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (293.92, 113.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (221, 353, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025) [M::mem_pestat] mean and std.dev: (363.46, 171.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1293) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 347, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (292.32, 133.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1075) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 146, #reads = 1071937 mem = 536884120 peak = 1343528960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 284419, 2, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 296, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943) [M::mem_pestat] mean and std.dev: (318.31, 139.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1190) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (226, 361, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033) [M::mem_pestat] mean and std.dev: (369.07, 172.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1302) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (292, 396, 535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021) [M::mem_pestat] mean and std.dev: (397.57, 142.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1264) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681914 reads in 377.148 CPU sec, 11.975 real sec [M::process] read 679944 sequences (100000259 bp) in 3.040 CPU sec, 3.044 real sec... [M::mem_process_seqs] Processed 681172 reads in 198.769 CPU sec, 6.335 real sec sorting block 147, #reads = 1073151 mem = 536879243 peak = 1343528960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 283930, 1, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 219, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (241.39, 106.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (229, 364, 499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039) [M::mem_pestat] mean and std.dev: (372.22, 173.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1309) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (266, 322, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (52, 587) [M::mem_pestat] mean and std.dev: (317.57, 112.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679736 sequences (100000121 bp) in 3.656 CPU sec, 3.724 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 284088, 5, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 234, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (330.15, 220.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1213) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (233, 369, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043) [M::mem_pestat] mean and std.dev: (376.02, 173.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1313) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 276, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (311.96, 138.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679972 sequences (100000295 bp) in 2.658 CPU sec, 2.665 real sec... [M::mem_process_seqs] Processed 680622 reads in 240.738 CPU sec, 7.651 real sec [M::mem_process_seqs] Processed 679944 reads in 167.346 CPU sec, 5.303 real sec sorting block 148, #reads = 1072470 mem = 536880340 peak = 1343528960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 284359, 4, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 368, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975) [M::mem_pestat] mean and std.dev: (371.66, 206.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1232) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (234, 369, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047) [M::mem_pestat] mean and std.dev: (377.08, 174.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1318) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (286, 361, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985) [M::mem_pestat] mean and std.dev: (387.69, 147.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1218) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678876 sequences (100000077 bp) in 3.407 CPU sec, 3.481 real sec... [M::process] read 678732 sequences (100000255 bp) in 2.610 CPU sec, 2.609 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 283745, 1, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (278, 453, 629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1331) [M::mem_pestat] mean and std.dev: (426.05, 183.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (230, 364, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046) [M::mem_pestat] mean and std.dev: (374.61, 175.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1318) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 344, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (328.43, 123.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 679736 reads in 237.236 CPU sec, 7.509 real sec [M::mem_process_seqs] Processed 679972 reads in 164.187 CPU sec, 5.208 real sec sorting block 149, #reads = 1071955 mem = 536880209 peak = 1343528960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 283570, 6, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 275, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947) [M::mem_pestat] mean and std.dev: (278.32, 133.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1204) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (242, 379, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055) [M::mem_pestat] mean and std.dev: (385.02, 176.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1326) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (257, 382, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (339.40, 140.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1001) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678658 sequences (100000223 bp) in 3.578 CPU sec, 3.638 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283821, 0, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 228, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (265.12, 142.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1177) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (242, 380, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1064) [M::mem_pestat] mean and std.dev: (386.53, 177.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 305, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981) [M::mem_pestat] mean and std.dev: (319.32, 185.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1248) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678678 sequences (100000190 bp) in 2.576 CPU sec, 2.583 real sec... [M::mem_process_seqs] Processed 678876 reads in 236.628 CPU sec, 7.508 real sec sorting block 150, #reads = 1073668 mem = 536879622 peak = 1343528960 [M::mem_process_seqs] Processed 678732 reads in 163.066 CPU sec, 5.170 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 283021, 0, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 238, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (232.42, 97.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (240, 379, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062) [M::mem_pestat] mean and std.dev: (384.86, 176.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1336) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 262, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (291.26, 132.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 151, #reads = 1073919 mem = 536879187 peak = 1343528960 [M::process] read 678422 sequences (100000127 bp) in 3.131 CPU sec, 3.195 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 282863, 2, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 238, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (237.63, 106.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (240, 376, 512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056) [M::mem_pestat] mean and std.dev: (383.57, 176.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1328) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 357, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998) [M::mem_pestat] mean and std.dev: (372.93, 162.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1252) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678316 sequences (100000266 bp) in 2.626 CPU sec, 2.632 real sec... [M::mem_process_seqs] Processed 678658 reads in 234.757 CPU sec, 7.473 real sec [M::mem_process_seqs] Processed 678678 reads in 163.181 CPU sec, 5.172 real sec sorting block 152, #reads = 1073671 mem = 536879371 peak = 1343528960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 283218, 9, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (222, 334, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987) [M::mem_pestat] mean and std.dev: (289.80, 114.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1242) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (240, 374, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050) [M::mem_pestat] mean and std.dev: (382.44, 175.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1320) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 336, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995) [M::mem_pestat] mean and std.dev: (363.90, 187.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1251) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678004 sequences (100000127 bp) in 3.333 CPU sec, 3.372 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282988, 1, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 215, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (202.82, 71.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (241, 376, 515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1063) [M::mem_pestat] mean and std.dev: (385.47, 178.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (256, 350, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 634) [M::mem_pestat] mean and std.dev: (303.08, 98.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677970 sequences (100000047 bp) in 2.365 CPU sec, 2.369 real sec... [M::mem_process_seqs] Processed 678422 reads in 229.652 CPU sec, 7.308 real sec [M::mem_process_seqs] Processed 678316 reads in 158.934 CPU sec, 5.038 real sec sorting block 153, #reads = 1073689 mem = 536878470 peak = 1343528960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 282874, 1, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 445, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1187) [M::mem_pestat] mean and std.dev: (380.53, 244.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 380, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1071) [M::mem_pestat] mean and std.dev: (388.89, 179.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1347) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 293, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (291.02, 154.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1080) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677904 sequences (100000085 bp) in 3.237 CPU sec, 3.273 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282548, 5, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 278, 1981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5481) [M::mem_pestat] mean and std.dev: (739.20, 795.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7231) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (242, 377, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1064) [M::mem_pestat] mean and std.dev: (386.70, 178.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (256, 289, 547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1129) [M::mem_pestat] mean and std.dev: (327.54, 153.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1420) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678160 sequences (100000274 bp) in 2.549 CPU sec, 2.552 real sec... [M::mem_process_seqs] Processed 678004 reads in 228.372 CPU sec, 7.245 real sec [M::mem_process_seqs] Processed 677970 reads in 156.475 CPU sec, 4.961 real sec sorting block 154, #reads = 1074355 mem = 536882547 peak = 1343528960 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282409, 5, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 240, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1095) [M::mem_pestat] mean and std.dev: (298.37, 250.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 378, 515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1057) [M::mem_pestat] mean and std.dev: (387.05, 177.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1328) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 378, 588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1352) [M::mem_pestat] mean and std.dev: (449.10, 334.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1788) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677562 sequences (100000129 bp) in 3.330 CPU sec, 3.389 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 282569, 4, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 340, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (335.00, 111.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 379, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068) [M::mem_pestat] mean and std.dev: (388.28, 179.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1343) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 298, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942) [M::mem_pestat] mean and std.dev: (290.80, 137.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1194) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677224 sequences (100000266 bp) in 2.493 CPU sec, 2.503 real sec... [M::mem_process_seqs] Processed 677904 reads in 225.299 CPU sec, 7.163 real sec sorting block 155, #reads = 1074722 mem = 536879508 peak = 1344135168 [M::mem_process_seqs] Processed 678160 reads in 155.270 CPU sec, 4.915 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 282769, 1, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 317, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1086) [M::mem_pestat] mean and std.dev: (293.96, 130.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1384) [M::mem_pestat] analyzing insert size distribution for orientation FR... sorting block 156, #reads = 1074430 mem = 536878976 peak = 1344135168 [M::mem_pestat] (25, 50, 75) percentile: (247, 383, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066) [M::mem_pestat] mean and std.dev: (391.67, 180.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1339) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 336, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966) [M::mem_pestat] mean and std.dev: (310.21, 163.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1221) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677566 sequences (100000267 bp) in 3.232 CPU sec, 3.298 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282195, 4, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 271, 729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1817) [M::mem_pestat] mean and std.dev: (334.24, 255.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2361) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 385, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1079) [M::mem_pestat] mean and std.dev: (394.50, 181.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1356) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 313, 608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1366) [M::mem_pestat] mean and std.dev: (323.68, 153.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1745) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677120 sequences (100000275 bp) in 2.534 CPU sec, 2.535 real sec... [M::mem_process_seqs] Processed 677562 reads in 227.248 CPU sec, 7.233 real sec [M::mem_process_seqs] Processed 677224 reads in 157.994 CPU sec, 5.005 real sec sorting block 157, #reads = 1075628 mem = 536880123 peak = 1344135168 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 282509, 2, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 277, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (244.62, 75.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 385, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1086) [M::mem_pestat] mean and std.dev: (394.34, 182.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1366) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 284, 561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1201) [M::mem_pestat] mean and std.dev: (340.88, 160.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1521) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677268 sequences (100000274 bp) in 3.580 CPU sec, 3.626 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 282465, 5, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 217, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015) [M::mem_pestat] mean and std.dev: (315.84, 180.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1290) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 388, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1089) [M::mem_pestat] mean and std.dev: (397.66, 184.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1369) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (258, 517, 1339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3501) [M::mem_pestat] mean and std.dev: (505.22, 401.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4582) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676818 sequences (100000221 bp) in 2.498 CPU sec, 2.502 real sec... [M::mem_process_seqs] Processed 677566 reads in 215.359 CPU sec, 6.822 real sec [M::mem_process_seqs] Processed 677120 reads in 147.458 CPU sec, 4.659 real sec sorting block 158, #reads = 1075238 mem = 536877544 peak = 1344135168 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 281971, 1, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (240, 326, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062) [M::mem_pestat] mean and std.dev: (372.62, 187.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1336) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (251, 390, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1091) [M::mem_pestat] mean and std.dev: (399.49, 185.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1371) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (287, 394, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995) [M::mem_pestat] mean and std.dev: (367.35, 119.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1231) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 674338 sequences (99439757 bp) in 3.212 CPU sec, 3.277 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282257, 2, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 253, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (269.79, 118.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1090) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (251, 390, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1097) [M::mem_pestat] mean and std.dev: (401.57, 187.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 268, 611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1451) [M::mem_pestat] mean and std.dev: (372.24, 282.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1871) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677268 reads in 214.452 CPU sec, 6.888 real sec [M::mem_process_seqs] Processed 676818 reads in 145.432 CPU sec, 4.680 real sec sorting block 159, #reads = 1075324 mem = 536879899 peak = 1344135168 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 281449, 2, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 318, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (307.68, 161.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1083) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 383, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1087) [M::mem_pestat] mean and std.dev: (393.92, 185.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1368) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 370, 587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1367) [M::mem_pestat] mean and std.dev: (371.29, 197.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1757) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 674338 reads in 123.827 CPU sec, 4.135 real sec sorting block 160, #reads = 1077388 mem = 536881404 peak = 1352945664 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 00050657 features: 1f8bfbff fffa320b extended: d19f47ab 00000818 amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8259CL CPU @ 2.50GHz threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 173177540 overall: 69929484288 mem 32213.397 user 324.065 sys 1048.470 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_3LU-T1-TDNA-1_A23MHWWLT4_1.L005\tPU:L005\tSM:1136_3LU-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_3LU-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1136_3LU-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 1136_3LU-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 1048.512 sec; CPU: 32537.471 sec sorting block 161, #reads = 650708 mem = 324674267 peak = 1352945664 uniq header count 1 merging 162 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 00050657 features: 1f8bfbff fffa320b extended: d19f47ab 00000818 amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8259CL CPU @ 2.50GHz threads: 31 max 48 algo: util-sort license: sentieon:util=1 output file size: 10268012553 output reads: 174116302 bam_mem_sort: 162 calls 165.515 user 1.151 sys 167.452 real bam_write: 162 calls 138.166 user 8.217 sys 181.619 real execute: 1 calls 58.617 user 1.688 sys 1160.159 real merge_files: 1 calls 58.574 user 1.663 sys 111.086 real parse_chunk: 2586 calls 223.932 user 45.779 sys 277.914 real read_chunk: 6971 calls 111.499 user 58.104 sys 1038.833 real sort_block: 1 calls 68.691 user 56.702 sys 986.345 real write_chunk: 4383 calls 57.767 user 1.308 sys 109.572 real overall: 3411505152 mem 4260.561 user 147.651 sys 1160.182 real ls: cannot access '*.cram': No such file or directory