.command.log - Workdir - DAQ:SENTIEON_BWAMEM (1029_IWL-T1-TDNA-1_A23MHWWLT4_1;L005;0001)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a2/034b70d763b0cf87179dabcab7da7c/.command.log
Size: 76.9 KB
Task: DAQ:SENTIEON_BWAMEM (1029_IWL-T1-TDNA-1_A23MHWWLT4_1;L005;0001)
Attempt
Content

  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a2/034b70d763b0cf87179dabcab7da7c/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/bd/af7471994ae0444837bb8fa836a452/1029_IWL-T1-TDNA-1_A23MHWWLT4_1/1029_IWL-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a2/034b70d763b0cf87179dabcab7da7c/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/bd/af7471994ae0444837bb8fa836a452/1029_IWL-T1-TDNA-1_A23MHWWLT4_1/1029_IWL-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1029_IWL-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2961.992 CPU sec, 121.346 real sec
[M::process] read 770120 sequences (100000064 bp) in 1.223 CPU sec, 1.230 real sec...
[M::process] read 769160 sequences (100000144 bp) in 1.279 CPU sec, 1.280 real sec...
[M::process] read 768402 sequences (100000098 bp) in 1.628 CPU sec, 1.627 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 304084, 12, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 140, 748)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1976)
[M::mem_pestat] mean and std.dev: (298.64, 365.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2590)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 147, 200)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378)
[M::mem_pestat] mean and std.dev: (159.69, 65.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 467)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (267, 761, 3678)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10500)
[M::mem_pestat] mean and std.dev: (1633.83, 1749.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 13911)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 623, 851)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2219)
[M::mem_pestat] mean and std.dev: (467.13, 375.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2903)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 768438 sequences (100000237 bp) in 1.620 CPU sec, 1.617 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 303882, 16, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 325, 1411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3927)
[M::mem_pestat] mean and std.dev: (547.06, 553.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5185)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 148, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381)
[M::mem_pestat] mean and std.dev: (160.75, 66.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 471)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (209, 389, 1141)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3005)
[M::mem_pestat] mean and std.dev: (634.50, 460.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3937)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 457, 2159)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6123)
[M::mem_pestat] mean and std.dev: (1132.36, 1186.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8105)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 304123, 4, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 232, 800)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1980)
[M::mem_pestat] mean and std.dev: (434.53, 353.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2570)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 148, 202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384)
[M::mem_pestat] mean and std.dev: (161.29, 67.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 475)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 217, 672)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1714)
[M::mem_pestat] mean and std.dev: (339.27, 272.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2235)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 770120 reads in 304.186 CPU sec, 9.693 real sec
[M::mem_process_seqs] Processed 769160 reads in 224.978 CPU sec, 7.260 real sec
[M::mem_process_seqs] Processed 768402 reads in 248.456 CPU sec, 8.062 real sec
sorting block 0, #reads = 1110437 mem = 536879618 peak = 573919232
[M::process] read 767490 sequences (100000216 bp) in 2.136 CPU sec, 2.275 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 304541, 7, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 244, 687)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1801)
[M::mem_pestat] mean and std.dev: (384.06, 353.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2358)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 148, 202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384)
[M::mem_pestat] mean and std.dev: (161.34, 67.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 475)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (135, 194, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (294.56, 246.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 1, #reads = 1112286 mem = 536880585 peak = 728059904
[M::process] read 766018 sequences (100000181 bp) in 2.272 CPU sec, 2.414 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 304535, 11, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 222, 673)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1739)
[M::mem_pestat] mean and std.dev: (310.41, 240.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2272)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 149, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387)
[M::mem_pestat] mean and std.dev: (161.87, 67.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 479)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (752, 915, 2318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5450)
[M::mem_pestat] mean and std.dev: (1122.40, 1085.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7016)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 424, 5298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15558)
[M::mem_pestat] mean and std.dev: (1657.00, 2169.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 20688)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 766476 sequences (100000074 bp) in 1.820 CPU sec, 1.820 real sec...
[M::mem_process_seqs] Processed 768438 reads in 216.291 CPU sec, 6.921 real sec
[M::mem_process_seqs] Processed 767490 reads in 142.798 CPU sec, 4.584 real sec
sorting block 2, #reads = 1114154 mem = 536879977 peak = 728059904
[M::process] read 765404 sequences (100000130 bp) in 2.378 CPU sec, 2.516 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 303416, 12, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 252, 1062)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2838)
[M::mem_pestat] mean and std.dev: (529.85, 400.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3726)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391)
[M::mem_pestat] mean and std.dev: (163.25, 68.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (114, 271, 902)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2478)
[M::mem_pestat] mean and std.dev: (387.55, 417.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3266)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 344, 1698)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4606)
[M::mem_pestat] mean and std.dev: (911.53, 1050.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6060)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 764188 sequences (100000207 bp) in 1.496 CPU sec, 1.501 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 303265, 7, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (122, 211, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (230.89, 133.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391)
[M::mem_pestat] mean and std.dev: (163.61, 68.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (116, 432, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1292)
[M::mem_pestat] mean and std.dev: (322.20, 183.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1684)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 303931, 12, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 611, 1726)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4836)
[M::mem_pestat] mean and std.dev: (1005.70, 1325.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6391)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388)
[M::mem_pestat] mean and std.dev: (162.85, 68.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 480)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (329, 830, 2354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6404)
[M::mem_pestat] mean and std.dev: (1396.50, 1517.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8429)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 218, 675)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1715)
[M::mem_pestat] mean and std.dev: (389.88, 398.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2235)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 766018 reads in 301.350 CPU sec, 9.710 real sec
[M::mem_process_seqs] Processed 765404 reads in 226.622 CPU sec, 7.338 real sec
[M::mem_process_seqs] Processed 766476 reads in 248.457 CPU sec, 8.095 real sec
sorting block 3, #reads = 1110680 mem = 536880118 peak = 728059904
[M::process] read 762604 sequences (100000251 bp) in 2.854 CPU sec, 3.058 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 302791, 12, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 327, 693)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1825)
[M::mem_pestat] mean and std.dev: (502.70, 469.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2391)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 151, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397)
[M::mem_pestat] mean and std.dev: (164.76, 70.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 492)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (213, 415, 1930)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5364)
[M::mem_pestat] mean and std.dev: (781.00, 846.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7081)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 217, 1451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4051)
[M::mem_pestat] mean and std.dev: (604.05, 605.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5351)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 764188 reads in 124.550 CPU sec, 4.160 real sec
sorting block 4, #reads = 1112285 mem = 536878755 peak = 728059904
[M::process] read 762568 sequences (100000134 bp) in 2.476 CPU sec, 2.676 real sec...
sorting block 5, #reads = 1110811 mem = 536877724 peak = 728059904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 302451, 8, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (223, 248, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (260.36, 88.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 152, 209)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401)
[M::mem_pestat] mean and std.dev: (166.28, 71.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 497)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 263, 2594)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7400)
[M::mem_pestat] mean and std.dev: (1266.06, 1585.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9803)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 761684 sequences (100000017 bp) in 2.381 CPU sec, 2.516 real sec...
[M::process] read 761842 sequences (100000233 bp) in 1.943 CPU sec, 1.958 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 302221, 9, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 373, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (325.50, 179.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 152, 208)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398)
[M::mem_pestat] mean and std.dev: (165.73, 70.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 493)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 209, 2035)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5799)
[M::mem_pestat] mean and std.dev: (815.70, 981.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7681)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 762604 reads in 228.820 CPU sec, 7.314 real sec
[M::mem_process_seqs] Processed 762568 reads in 147.640 CPU sec, 4.715 real sec
sorting block 6, #reads = 1108421 mem = 536877327 peak = 728059904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 301685, 12, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 342, 920)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2382)
[M::mem_pestat] mean and std.dev: (454.05, 397.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3113)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 152, 208)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398)
[M::mem_pestat] mean and std.dev: (166.01, 69.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 493)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 323, 621)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1533)
[M::mem_pestat] mean and std.dev: (261.70, 202.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1989)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 451, 2087)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5953)
[M::mem_pestat] mean and std.dev: (1472.92, 1932.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9201)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 762258 sequences (100000122 bp) in 3.189 CPU sec, 3.373 real sec...
[M::process] read 767396 sequences (100000008 bp) in 2.099 CPU sec, 2.099 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 301571, 12, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 235, 760)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2026)
[M::mem_pestat] mean and std.dev: (455.40, 437.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2659)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 152, 208)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398)
[M::mem_pestat] mean and std.dev: (165.76, 69.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 493)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (343, 1100, 1819)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4771)
[M::mem_pestat] mean and std.dev: (963.55, 686.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6247)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (132, 222, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501)
[M::mem_pestat] mean and std.dev: (181.80, 58.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 624)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 761684 reads in 230.569 CPU sec, 7.366 real sec
sorting block 7, #reads = 1107265 mem = 536882714 peak = 728059904
[M::mem_process_seqs] Processed 761842 reads in 153.346 CPU sec, 4.911 real sec
sorting block 8, #reads = 1103670 mem = 536876034 peak = 728059904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 301734, 15, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 170, 565)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1415)
[M::mem_pestat] mean and std.dev: (245.12, 176.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1840)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 152, 208)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398)
[M::mem_pestat] mean and std.dev: (165.63, 69.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 493)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (326, 449, 706)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1466)
[M::mem_pestat] mean and std.dev: (507.57, 290.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1846)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 282, 1206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3252)
[M::mem_pestat] mean and std.dev: (562.78, 489.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4275)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 768740 sequences (100000147 bp) in 3.001 CPU sec, 3.165 real sec...
[M::process] read 768186 sequences (100000073 bp) in 1.731 CPU sec, 1.738 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 303535, 13, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (235, 433, 1137)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2941)
[M::mem_pestat] mean and std.dev: (652.88, 643.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3843)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 149, 202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384)
[M::mem_pestat] mean and std.dev: (161.51, 67.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 475)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (35, 295, 1550)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4580)
[M::mem_pestat] mean and std.dev: (929.92, 1588.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7282)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 303, 1403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3895)
[M::mem_pestat] mean and std.dev: (562.53, 669.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5141)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 762258 reads in 237.753 CPU sec, 7.611 real sec
[M::mem_process_seqs] Processed 767396 reads in 150.641 CPU sec, 4.850 real sec
sorting block 9, #reads = 1104309 mem = 536877947 peak = 728059904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 303590, 18, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 439, 1606)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4462)
[M::mem_pestat] mean and std.dev: (695.88, 734.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5890)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 148, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381)
[M::mem_pestat] mean and std.dev: (160.64, 66.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 471)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 767, 855)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2287)
[M::mem_pestat] mean and std.dev: (526.47, 548.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3003)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 282, 1113)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2965)
[M::mem_pestat] mean and std.dev: (666.18, 701.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3891)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 767574 sequences (100000092 bp) in 3.192 CPU sec, 3.404 real sec...
[M::process] read 767524 sequences (100000263 bp) in 2.098 CPU sec, 2.104 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 304015, 8, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 307, 843)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2225)
[M::mem_pestat] mean and std.dev: (503.67, 492.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2916)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 148, 202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384)
[M::mem_pestat] mean and std.dev: (161.45, 67.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 475)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 172, 1876)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5308)
[M::mem_pestat] mean and std.dev: (696.46, 805.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7024)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 768740 reads in 217.939 CPU sec, 6.963 real sec
[M::mem_process_seqs] Processed 768186 reads in 139.312 CPU sec, 4.458 real sec
sorting block 10, #reads = 1110959 mem = 536880224 peak = 728059904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 304024, 9, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 202, 1844)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5254)
[M::mem_pestat] mean and std.dev: (659.79, 717.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6959)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 149, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387)
[M::mem_pestat] mean and std.dev: (161.89, 67.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 479)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 208, 621)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1503)
[M::mem_pestat] mean and std.dev: (310.73, 225.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1944)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 766138 sequences (100000228 bp) in 2.974 CPU sec, 3.156 real sec...
[M::process] read 765642 sequences (100000208 bp) in 2.100 CPU sec, 2.108 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 304220, 6, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (113, 149, 913)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2513)
[M::mem_pestat] mean and std.dev: (384.18, 568.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3313)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 149, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387)
[M::mem_pestat] mean and std.dev: (161.81, 67.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 479)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 767574 reads in 209.872 CPU sec, 6.698 real sec
sorting block 11, #reads = 1113569 mem = 536880025 peak = 728059904
[M::mem_process_seqs] Processed 767524 reads in 136.559 CPU sec, 4.356 real sec
sorting block 12, #reads = 1112735 mem = 536882547 peak = 728059904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 303374, 6, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 160, 610)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1546)
[M::mem_pestat] mean and std.dev: (286.14, 224.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2014)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 149, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388)
[M::mem_pestat] mean and std.dev: (162.70, 68.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 480)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 366, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693)
[M::mem_pestat] mean and std.dev: (283.25, 104.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 765638 sequences (100000229 bp) in 2.986 CPU sec, 3.169 real sec...
[M::process] read 765298 sequences (100000036 bp) in 2.048 CPU sec, 2.052 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 303417, 7, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 200, 857)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2267)
[M::mem_pestat] mean and std.dev: (408.46, 430.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2972)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391)
[M::mem_pestat] mean and std.dev: (163.47, 68.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 263, 968)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2560)
[M::mem_pestat] mean and std.dev: (533.76, 452.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3356)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 766138 reads in 212.636 CPU sec, 6.801 real sec
[M::mem_process_seqs] Processed 765642 reads in 137.643 CPU sec, 4.395 real sec
sorting block 13, #reads = 1111936 mem = 536879138 peak = 728059904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 303231, 6, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 186, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (192.53, 100.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391)
[M::mem_pestat] mean and std.dev: (163.26, 68.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 488, 1227)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3375)
[M::mem_pestat] mean and std.dev: (758.68, 870.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4449)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 764488 sequences (100000128 bp) in 3.291 CPU sec, 3.463 real sec...
[M::process] read 763020 sequences (100000204 bp) in 1.885 CPU sec, 1.895 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 303128, 6, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 143, 202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350)
[M::mem_pestat] mean and std.dev: (149.92, 42.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 424)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391)
[M::mem_pestat] mean and std.dev: (163.46, 68.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 264, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 505)
[M::mem_pestat] mean and std.dev: (249.55, 48.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 593)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 765638 reads in 206.106 CPU sec, 6.595 real sec
[M::mem_process_seqs] Processed 765298 reads in 131.903 CPU sec, 4.214 real sec
sorting block 14, #reads = 1108844 mem = 536879328 peak = 728059904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 303334, 6, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 289, 622)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1558)
[M::mem_pestat] mean and std.dev: (371.58, 306.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2026)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 151, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394)
[M::mem_pestat] mean and std.dev: (164.07, 69.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 488)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 154, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (196.71, 137.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 762800 sequences (100000001 bp) in 3.326 CPU sec, 3.545 real sec...
[M::process] read 762012 sequences (100000180 bp) in 1.529 CPU sec, 1.529 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 302678, 9, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (116, 141, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383)
[M::mem_pestat] mean and std.dev: (148.46, 56.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 472)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 151, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395)
[M::mem_pestat] mean and std.dev: (164.83, 69.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 489)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 398, 1262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3488)
[M::mem_pestat] mean and std.dev: (493.61, 447.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4601)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 764488 reads in 204.904 CPU sec, 6.541 real sec
sorting block 15, #reads = 1110702 mem = 536880824 peak = 728059904
[M::mem_process_seqs] Processed 763020 reads in 133.165 CPU sec, 4.261 real sec
sorting block 16, #reads = 1111071 mem = 536889526 peak = 728059904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 301694, 3, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 185, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (260.00, 225.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1319)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 151, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392)
[M::mem_pestat] mean and std.dev: (164.68, 68.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 485)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 188, 810)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2124)
[M::mem_pestat] mean and std.dev: (392.42, 363.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2781)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 762742 sequences (100000214 bp) in 3.092 CPU sec, 3.257 real sec...
[M::process] read 762394 sequences (100000145 bp) in 1.720 CPU sec, 1.717 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 301759, 9, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 282, 980)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2630)
[M::mem_pestat] mean and std.dev: (464.69, 443.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3455)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 152, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395)
[M::mem_pestat] mean and std.dev: (165.36, 69.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 489)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 762800 reads in 207.289 CPU sec, 6.616 real sec
[M::mem_process_seqs] Processed 762012 reads in 133.140 CPU sec, 4.259 real sec
sorting block 17, #reads = 1108139 mem = 536885594 peak = 728059904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 302072, 15, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 348, 1137)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3111)
[M::mem_pestat] mean and std.dev: (459.44, 469.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4098)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 152, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395)
[M::mem_pestat] mean and std.dev: (165.03, 69.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 489)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (644, 644, 3174)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8234)
[M::mem_pestat] mean and std.dev: (1575.93, 1898.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10764)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 561, 1984)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5536)
[M::mem_pestat] mean and std.dev: (801.46, 793.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7312)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 770002 sequences (100000153 bp) in 3.043 CPU sec, 3.264 real sec...
[M::process] read 767664 sequences (100000169 bp) in 1.630 CPU sec, 1.630 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 301885, 11, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (121, 373, 754)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2020)
[M::mem_pestat] mean and std.dev: (371.77, 303.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2653)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 151, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395)
[M::mem_pestat] mean and std.dev: (164.88, 69.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 489)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (277, 808, 1095)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2731)
[M::mem_pestat] mean and std.dev: (548.11, 373.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3549)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 280, 2026)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5698)
[M::mem_pestat] mean and std.dev: (858.00, 1216.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7534)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 762742 reads in 212.601 CPU sec, 6.791 real sec
sorting block 18, #reads = 1108005 mem = 536887090 peak = 728059904
[M::mem_process_seqs] Processed 762394 reads in 141.050 CPU sec, 4.515 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 303923, 14, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 300, 670)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1690)
[M::mem_pestat] mean and std.dev: (423.90, 334.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2200)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 147, 200)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378)
[M::mem_pestat] mean and std.dev: (159.68, 65.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 467)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (419, 623, 1016)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2210)
[M::mem_pestat] mean and std.dev: (579.25, 315.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2807)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
sorting block 19, #reads = 1106313 mem = 536877472 peak = 731836416
[M::process] read 766796 sequences (100000084 bp) in 3.572 CPU sec, 3.731 real sec...
[M::process] read 766440 sequences (100000255 bp) in 1.728 CPU sec, 1.737 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 303890, 9, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 805, 1165)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3167)
[M::mem_pestat] mean and std.dev: (787.94, 640.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4168)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 149, 202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384)
[M::mem_pestat] mean and std.dev: (161.58, 67.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 475)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (316, 360, 949)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2215)
[M::mem_pestat] mean and std.dev: (475.88, 320.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2848)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 770002 reads in 208.176 CPU sec, 6.644 real sec
[M::mem_process_seqs] Processed 767664 reads in 137.022 CPU sec, 4.380 real sec
sorting block 20, #reads = 1110704 mem = 536882126 peak = 731836416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 303222, 9, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 418, 925)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2521)
[M::mem_pestat] mean and std.dev: (401.14, 338.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3319)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 149, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387)
[M::mem_pestat] mean and std.dev: (162.26, 67.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 479)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 783, 1617)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4509)
[M::mem_pestat] mean and std.dev: (1202.44, 1268.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6278)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 765884 sequences (100000008 bp) in 3.152 CPU sec, 3.340 real sec...
[M::process] read 765702 sequences (100000242 bp) in 1.529 CPU sec, 1.538 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 303926, 4, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 149, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388)
[M::mem_pestat] mean and std.dev: (162.41, 67.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 480)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 165, 657)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1663)
[M::mem_pestat] mean and std.dev: (387.86, 285.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2166)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 766796 reads in 203.044 CPU sec, 6.488 real sec
sorting block 21, #reads = 1111139 mem = 536887827 peak = 731836416
[M::process] read 765900 sequences (100000301 bp) in 2.196 CPU sec, 2.201 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 303409, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 149, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388)
[M::mem_pestat] mean and std.dev: (162.51, 67.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 480)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 265, 728)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1826)
[M::mem_pestat] mean and std.dev: (262.22, 178.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2375)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 765884 reads in 108.897 CPU sec, 3.461 real sec
[M::mem_process_seqs] Processed 766440 reads in 276.236 CPU sec, 8.831 real sec
sorting block 22, #reads = 1112012 mem = 536877974 peak = 731836416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 303225, 11, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 256, 989)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2653)
[M::mem_pestat] mean and std.dev: (461.57, 395.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3485)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391)
[M::mem_pestat] mean and std.dev: (163.23, 68.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (636, 1140, 4687)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12789)
[M::mem_pestat] mean and std.dev: (2441.45, 2501.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16840)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 281, 789)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1955)
[M::mem_pestat] mean and std.dev: (334.40, 224.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2538)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 764402 sequences (100000281 bp) in 2.857 CPU sec, 3.020 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 303675, 10, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (120, 134, 814)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2202)
[M::mem_pestat] mean and std.dev: (381.67, 423.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2896)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388)
[M::mem_pestat] mean and std.dev: (162.65, 68.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 480)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (63, 296, 1215)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3519)
[M::mem_pestat] mean and std.dev: (782.70, 941.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4671)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 381, 850)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2178)
[M::mem_pestat] mean and std.dev: (373.71, 283.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2842)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 762936 sequences (100000011 bp) in 1.751 CPU sec, 1.751 real sec...
[M::mem_process_seqs] Processed 765702 reads in 219.944 CPU sec, 7.022 real sec
sorting block 23, #reads = 1110674 mem = 536879620 peak = 731836416
[M::mem_process_seqs] Processed 765900 reads in 132.351 CPU sec, 4.234 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 302822, 7, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 171, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (36, 316)
[M::mem_pestat] mean and std.dev: (152.93, 41.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 372)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394)
[M::mem_pestat] mean and std.dev: (164.04, 69.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 488)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 195, 680)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1690)
[M::mem_pestat] mean and std.dev: (259.42, 192.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2195)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 761974 sequences (100000045 bp) in 2.874 CPU sec, 3.107 real sec...
sorting block 24, #reads = 1111692 mem = 536881023 peak = 731836416
[M::process] read 763334 sequences (100000135 bp) in 1.742 CPU sec, 1.751 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 303170, 8, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 392, 977)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2541)
[M::mem_pestat] mean and std.dev: (533.12, 420.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3323)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 151, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395)
[M::mem_pestat] mean and std.dev: (164.84, 69.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 489)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 292, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1064)
[M::mem_pestat] mean and std.dev: (323.50, 180.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1347)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 764402 reads in 202.046 CPU sec, 6.457 real sec
[M::mem_process_seqs] Processed 762936 reads in 133.824 CPU sec, 4.272 real sec
sorting block 25, #reads = 1109671 mem = 536891492 peak = 731836416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 302029, 10, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 189, 985)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2635)
[M::mem_pestat] mean and std.dev: (621.95, 764.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3680)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 152, 208)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398)
[M::mem_pestat] mean and std.dev: (165.60, 69.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 493)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (515, 1996, 2848)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7514)
[M::mem_pestat] mean and std.dev: (1462.67, 1017.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9847)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 333, 664)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1574)
[M::mem_pestat] mean and std.dev: (438.06, 338.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2029)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 763630 sequences (100000232 bp) in 3.340 CPU sec, 3.535 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 302166, 7, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 212, 1222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3386)
[M::mem_pestat] mean and std.dev: (367.94, 409.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4468)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 151, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392)
[M::mem_pestat] mean and std.dev: (164.24, 68.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 485)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 291, 1074)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2898)
[M::mem_pestat] mean and std.dev: (717.18, 774.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3813)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 762212 sequences (100000116 bp) in 2.089 CPU sec, 2.097 real sec...
[M::mem_process_seqs] Processed 761974 reads in 200.971 CPU sec, 6.391 real sec
[M::mem_process_seqs] Processed 763334 reads in 131.910 CPU sec, 4.202 real sec
sorting block 26, #reads = 1109014 mem = 536890047 peak = 731836416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 302406, 10, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (301, 768, 1199)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2995)
[M::mem_pestat] mean and std.dev: (710.88, 415.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3893)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 151, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392)
[M::mem_pestat] mean and std.dev: (164.14, 68.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 485)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (347, 970, 970)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2216)
[M::mem_pestat] mean and std.dev: (760.90, 385.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2839)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (122, 222, 2137)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6167)
[M::mem_pestat] mean and std.dev: (876.44, 1278.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8182)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 762872 sequences (100000079 bp) in 2.836 CPU sec, 3.013 real sec...
[M::process] read 768712 sequences (100000030 bp) in 2.120 CPU sec, 2.119 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 301707, 15, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 1439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4013)
[M::mem_pestat] mean and std.dev: (643.88, 928.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5300)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 152, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395)
[M::mem_pestat] mean and std.dev: (165.42, 69.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 489)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (443, 875, 2345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6149)
[M::mem_pestat] mean and std.dev: (1306.64, 1454.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8051)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 763630 reads in 209.122 CPU sec, 6.682 real sec
sorting block 27, #reads = 1107618 mem = 536880218 peak = 731836416
[M::mem_process_seqs] Processed 762212 reads in 138.176 CPU sec, 4.418 real sec
sorting block 28, #reads = 1106788 mem = 536883632 peak = 767467520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 301979, 9, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 700, 1451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4093)
[M::mem_pestat] mean and std.dev: (831.27, 763.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5414)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 151, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395)
[M::mem_pestat] mean and std.dev: (164.95, 69.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 489)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 337, 1207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3303)
[M::mem_pestat] mean and std.dev: (748.56, 761.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4351)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 768530 sequences (100000179 bp) in 3.015 CPU sec, 3.161 real sec...
[M::process] read 767070 sequences (100000145 bp) in 2.076 CPU sec, 2.074 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 303632, 9, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (217, 509, 1156)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3034)
[M::mem_pestat] mean and std.dev: (618.75, 570.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3973)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 148, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381)
[M::mem_pestat] mean and std.dev: (160.47, 66.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 471)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 197, 1365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3773)
[M::mem_pestat] mean and std.dev: (604.50, 741.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4977)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 762872 reads in 210.109 CPU sec, 6.701 real sec
[M::mem_process_seqs] Processed 768712 reads in 137.663 CPU sec, 4.389 real sec
sorting block 29, #reads = 1104578 mem = 536878705 peak = 767467520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 303973, 7, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 325, 1122)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3078)
[M::mem_pestat] mean and std.dev: (564.48, 647.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4056)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 148, 200)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378)
[M::mem_pestat] mean and std.dev: (160.32, 65.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 467)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (134, 197, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (168.29, 71.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1070)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 766092 sequences (100000053 bp) in 2.686 CPU sec, 2.845 real sec...
[M::process] read 765290 sequences (100000143 bp) in 1.707 CPU sec, 1.709 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 303898, 8, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 242, 548)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1280)
[M::mem_pestat] mean and std.dev: (303.31, 195.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1646)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 149, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385)
[M::mem_pestat] mean and std.dev: (161.84, 67.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 476)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 269, 627)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1581)
[M::mem_pestat] mean and std.dev: (386.22, 351.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2058)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 768530 reads in 200.614 CPU sec, 6.413 real sec
[M::mem_process_seqs] Processed 767070 reads in 130.306 CPU sec, 4.172 real sec
sorting block 30, #reads = 1110299 mem = 536880587 peak = 767467520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 303334, 8, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 189, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (270.46, 181.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1338)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388)
[M::mem_pestat] mean and std.dev: (162.67, 68.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 480)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 476, 1023)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2749)
[M::mem_pestat] mean and std.dev: (765.19, 731.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3690)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 765380 sequences (100000014 bp) in 2.954 CPU sec, 3.088 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 302965, 13, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391)
[M::mem_pestat] mean and std.dev: (163.30, 68.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (256, 415, 3058)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8662)
[M::mem_pestat] mean and std.dev: (1219.92, 1388.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11464)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 254, 578)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1438)
[M::mem_pestat] mean and std.dev: (324.00, 251.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1868)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 765646 sequences (100000086 bp) in 1.620 CPU sec, 1.621 real sec...
[M::mem_process_seqs] Processed 766092 reads in 200.519 CPU sec, 6.461 real sec
sorting block 31, #reads = 1111100 mem = 536877629 peak = 767467520
[M::mem_process_seqs] Processed 765290 reads in 124.655 CPU sec, 3.970 real sec
sorting block 32, #reads = 1110943 mem = 536879402 peak = 790982656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 303194, 13, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 508, 586)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1504)
[M::mem_pestat] mean and std.dev: (406.89, 312.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1963)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391)
[M::mem_pestat] mean and std.dev: (163.29, 68.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 1942, 2848)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8200)
[M::mem_pestat] mean and std.dev: (1490.54, 1366.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10876)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 277, 1497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4097)
[M::mem_pestat] mean and std.dev: (649.37, 652.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5397)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 764110 sequences (100000141 bp) in 2.760 CPU sec, 2.935 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 303332, 6, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 151, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1175)
[M::mem_pestat] mean and std.dev: (193.33, 117.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1519)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391)
[M::mem_pestat] mean and std.dev: (163.36, 68.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 252, 1377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3733)
[M::mem_pestat] mean and std.dev: (513.40, 464.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4911)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 762838 sequences (100000117 bp) in 1.447 CPU sec, 1.452 real sec...
[M::mem_process_seqs] Processed 765380 reads in 179.836 CPU sec, 5.751 real sec
[M::mem_process_seqs] Processed 765646 reads in 116.156 CPU sec, 3.706 real sec
sorting block 33, #reads = 1111452 mem = 536879993 peak = 790982656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 302853, 10, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (247, 610, 883)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2155)
[M::mem_pestat] mean and std.dev: (443.00, 256.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2791)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 151, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394)
[M::mem_pestat] mean and std.dev: (164.19, 69.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 488)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 558, 1755)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4861)
[M::mem_pestat] mean and std.dev: (1096.33, 1354.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6513)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 280, 1256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3428)
[M::mem_pestat] mean and std.dev: (590.92, 643.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4514)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 761354 sequences (100000175 bp) in 2.549 CPU sec, 2.709 real sec...