cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_Kh-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1615.166 CPU sec, 60.753 real sec
[M::process] read 673400 sequences (100000108 bp) in 0.967 CPU sec, 0.976 real sec...
[M::process] read 672272 sequences (100000001 bp) in 1.209 CPU sec, 1.213 real sec...
[M::process] read 672134 sequences (100000123 bp) in 1.195 CPU sec, 1.226 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278995, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 352, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (373.05, 166.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671888 sequences (100000083 bp) in 1.162 CPU sec, 1.191 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278469, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 367, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (385.55, 168.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278980, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 369, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (386.77, 168.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673400 reads in 218.412 CPU sec, 7.004 real sec
[M::mem_process_seqs] Processed 672272 reads in 145.295 CPU sec, 4.699 real sec
[M::mem_process_seqs] Processed 672134 reads in 156.773 CPU sec, 5.084 real sec
sorting block 0, #reads = 1075329 mem = 536877440 peak = 598986752
[M::process] read 671632 sequences (100000119 bp) in 1.698 CPU sec, 1.947 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 278306, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 373, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (389.11, 167.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671518 sequences (100000051 bp) in 1.192 CPU sec, 1.201 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278655, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 375, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (390.34, 167.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671414 sequences (100000159 bp) in 1.100 CPU sec, 1.105 real sec...
[M::mem_process_seqs] Processed 671888 reads in 133.912 CPU sec, 4.248 real sec
sorting block 1, #reads = 1075089 mem = 536878565 peak = 784584704
[M::mem_process_seqs] Processed 671632 reads in 82.802 CPU sec, 2.613 real sec
sorting block 2, #reads = 1075164 mem = 536883752 peak = 901427200
[M::process] read 670886 sequences (100000042 bp) in 1.519 CPU sec, 1.605 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278157, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 377, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (392.92, 168.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671026 sequences (100000067 bp) in 1.204 CPU sec, 1.208 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277715, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 382, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (396.88, 169.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 277838, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 385, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (400.27, 171.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671518 reads in 189.210 CPU sec, 6.044 real sec
[M::mem_process_seqs] Processed 671414 reads in 139.428 CPU sec, 4.470 real sec
[M::mem_process_seqs] Processed 670886 reads in 150.812 CPU sec, 4.857 real sec
sorting block 3, #reads = 1074409 mem = 536879294 peak = 901427200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278376, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 381, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (398.36, 171.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670852 sequences (100000058 bp) in 1.976 CPU sec, 2.125 real sec...
[M::mem_process_seqs] Processed 671026 reads in 73.714 CPU sec, 2.425 real sec
sorting block 4, #reads = 1074471 mem = 536877414 peak = 901427200
[M::process] read 670788 sequences (100000133 bp) in 1.827 CPU sec, 1.998 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278107, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 390, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (403.73, 172.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670580 sequences (100000211 bp) in 1.180 CPU sec, 1.179 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278065, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 388, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (402.47, 171.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670508 sequences (100000061 bp) in 1.224 CPU sec, 1.226 real sec...
[M::mem_process_seqs] Processed 670852 reads in 133.315 CPU sec, 4.201 real sec
[M::mem_process_seqs] Processed 670788 reads in 82.994 CPU sec, 2.619 real sec
sorting block 5, #reads = 1075527 mem = 536886710 peak = 901427200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 277668, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 392, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (405.68, 172.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1280)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670532 sequences (100000018 bp) in 1.967 CPU sec, 2.099 real sec...
[M::process] read 670470 sequences (100000278 bp) in 1.210 CPU sec, 1.213 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277767, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 392, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (405.52, 172.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670580 reads in 134.897 CPU sec, 4.286 real sec
sorting block 6, #reads = 1074758 mem = 536879272 peak = 901427200
[M::mem_process_seqs] Processed 670508 reads in 83.478 CPU sec, 2.656 real sec
sorting block 7, #reads = 1075263 mem = 536879308 peak = 901427200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 277670, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 390, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (404.90, 172.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670662 sequences (100000184 bp) in 1.888 CPU sec, 2.002 real sec...
[M::process] read 670498 sequences (100000222 bp) in 1.240 CPU sec, 1.240 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277718, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 389, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (404.02, 171.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670532 reads in 136.303 CPU sec, 4.331 real sec
[M::mem_process_seqs] Processed 670470 reads in 84.899 CPU sec, 2.706 real sec
sorting block 8, #reads = 1073805 mem = 536878766 peak = 901427200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278256, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 384, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (399.73, 169.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670570 sequences (100000240 bp) in 1.953 CPU sec, 2.059 real sec...
[M::process] read 670690 sequences (100000236 bp) in 1.290 CPU sec, 1.295 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277906, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 381, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (397.55, 168.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670662 reads in 136.268 CPU sec, 4.327 real sec
[M::mem_process_seqs] Processed 670498 reads in 84.246 CPU sec, 2.679 real sec
sorting block 9, #reads = 1075274 mem = 536878537 peak = 901427200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278003, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 378, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (394.90, 167.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670904 sequences (100000180 bp) in 1.944 CPU sec, 2.068 real sec...
[M::process] read 670942 sequences (100000137 bp) in 1.280 CPU sec, 1.278 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277834, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 376, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (392.23, 165.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670570 reads in 135.682 CPU sec, 4.310 real sec
[M::mem_process_seqs] Processed 670690 reads in 84.091 CPU sec, 2.669 real sec
sorting block 10, #reads = 1074773 mem = 536880553 peak = 901427200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278345, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 373, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (390.14, 165.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670830 sequences (100000133 bp) in 2.220 CPU sec, 2.362 real sec...
[M::process] read 670760 sequences (100000104 bp) in 1.260 CPU sec, 1.264 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278088, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 374, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (390.52, 165.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670904 reads in 135.492 CPU sec, 4.302 real sec
sorting block 11, #reads = 1074993 mem = 536877892 peak = 901427200
[M::mem_process_seqs] Processed 670942 reads in 83.817 CPU sec, 2.651 real sec
sorting block 12, #reads = 1073915 mem = 536878708 peak = 942854144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278008, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 374, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (390.78, 165.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670858 sequences (100000105 bp) in 2.040 CPU sec, 2.160 real sec...
[M::process] read 670716 sequences (100000231 bp) in 1.310 CPU sec, 1.304 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278051, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 374, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (390.83, 165.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670830 reads in 134.901 CPU sec, 4.284 real sec
[M::mem_process_seqs] Processed 670760 reads in 83.521 CPU sec, 2.648 real sec
sorting block 13, #reads = 1074237 mem = 536878373 peak = 942854144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277665, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 376, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (392.51, 166.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670314 sequences (100000024 bp) in 2.079 CPU sec, 2.220 real sec...
[M::process] read 670334 sequences (100000244 bp) in 1.270 CPU sec, 1.271 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278565, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 379, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (395.25, 167.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670858 reads in 135.505 CPU sec, 4.305 real sec
[M::mem_process_seqs] Processed 670716 reads in 85.112 CPU sec, 2.699 real sec
sorting block 14, #reads = 1073494 mem = 536882198 peak = 942854144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278594, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 385, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (400.73, 169.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671808 sequences (100000122 bp) in 2.113 CPU sec, 2.243 real sec...
[M::process] read 672190 sequences (100000168 bp) in 1.280 CPU sec, 1.286 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278099, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 385, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (402.20, 170.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670314 reads in 132.510 CPU sec, 4.207 real sec
[M::mem_process_seqs] Processed 670334 reads in 83.305 CPU sec, 2.640 real sec
sorting block 15, #reads = 1072616 mem = 536879591 peak = 942854144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278790, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 370, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (388.07, 169.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671826 sequences (100000116 bp) in 2.076 CPU sec, 2.187 real sec...
[M::process] read 671716 sequences (100000123 bp) in 1.290 CPU sec, 1.289 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278573, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 369, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (387.66, 170.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671808 reads in 134.085 CPU sec, 4.257 real sec
sorting block 16, #reads = 1073111 mem = 536879428 peak = 942854144
[M::mem_process_seqs] Processed 672190 reads in 82.536 CPU sec, 2.617 real sec
sorting block 17, #reads = 1076664 mem = 536880746 peak = 981008384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278626, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 372, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (389.38, 169.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671598 sequences (100000164 bp) in 2.122 CPU sec, 2.253 real sec...
[M::process] read 671486 sequences (100000106 bp) in 1.310 CPU sec, 1.308 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277284, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 374, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (390.70, 169.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671826 reads in 133.181 CPU sec, 4.229 real sec
[M::mem_process_seqs] Processed 671716 reads in 82.708 CPU sec, 2.619 real sec
sorting block 18, #reads = 1076901 mem = 536883968 peak = 981008384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277616, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 377, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (392.89, 169.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671416 sequences (100000185 bp) in 2.134 CPU sec, 2.247 real sec...
[M::process] read 671272 sequences (100000257 bp) in 1.300 CPU sec, 1.303 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277807, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 380, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (395.04, 169.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671598 reads in 133.162 CPU sec, 4.229 real sec
[M::mem_process_seqs] Processed 671486 reads in 83.106 CPU sec, 2.632 real sec
sorting block 19, #reads = 1075978 mem = 536879099 peak = 981008384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278133, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 382, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (396.78, 170.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670898 sequences (100000282 bp) in 2.150 CPU sec, 2.289 real sec...
[M::process] read 670946 sequences (100000052 bp) in 1.310 CPU sec, 1.317 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278157, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 383, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (397.87, 170.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671416 reads in 134.222 CPU sec, 4.263 real sec
[M::mem_process_seqs] Processed 671272 reads in 83.524 CPU sec, 2.643 real sec
sorting block 20, #reads = 1076401 mem = 536880479 peak = 981008384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278456, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 386, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (400.90, 170.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670694 sequences (100000301 bp) in 2.177 CPU sec, 2.283 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278051, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 386, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (400.35, 170.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670636 sequences (100000086 bp) in 1.389 CPU sec, 1.390 real sec...
[M::mem_process_seqs] Processed 670898 reads in 131.096 CPU sec, 4.162 real sec
sorting block 21, #reads = 1076142 mem = 536878582 peak = 981008384
[M::process] read 670642 sequences (100000200 bp) in 1.541 CPU sec, 1.544 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277605, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 387, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (401.10, 169.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670694 reads in 76.903 CPU sec, 2.661 real sec
sorting block 22, #reads = 1076645 mem = 536881350 peak = 981008384
[M::mem_process_seqs] Processed 670946 reads in 176.871 CPU sec, 5.836 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277653, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 393, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (405.75, 171.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670588 sequences (100000136 bp) in 2.219 CPU sec, 2.389 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277927, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 393, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (405.87, 171.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670636 reads in 146.479 CPU sec, 4.656 real sec
[M::mem_process_seqs] Processed 670642 reads in 85.078 CPU sec, 2.686 real sec
sorting block 23, #reads = 1076467 mem = 536877778 peak = 981008384
[M::process] read 670472 sequences (100000282 bp) in 1.508 CPU sec, 1.541 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 277810, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 390, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (403.83, 170.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 24, #reads = 1074701 mem = 536877670 peak = 981008384
[M::process] read 670472 sequences (100000106 bp) in 1.843 CPU sec, 1.972 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277737, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 392, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (405.15, 170.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670748 sequences (100000169 bp) in 1.330 CPU sec, 1.324 real sec...
[M::mem_process_seqs] Processed 670588 reads in 134.778 CPU sec, 4.297 real sec
[M::process] read 670456 sequences (100000186 bp) in 1.506 CPU sec, 1.515 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277966, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 393, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (406.58, 171.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670472 reads in 79.084 CPU sec, 2.684 real sec
sorting block 25, #reads = 1074673 mem = 536879879 peak = 981008384
[M::mem_process_seqs] Processed 670472 reads in 186.343 CPU sec, 6.095 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278406, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 376, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (392.10, 165.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670618 sequences (100000015 bp) in 1.927 CPU sec, 2.065 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278453, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 382, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (397.31, 166.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670624 sequences (100000184 bp) in 1.320 CPU sec, 1.326 real sec...
[M::mem_process_seqs] Processed 670748 reads in 147.848 CPU sec, 4.707 real sec
sorting block 26, #reads = 1075471 mem = 536878584 peak = 981008384
[M::mem_process_seqs] Processed 670456 reads in 85.014 CPU sec, 2.691 real sec
sorting block 27, #reads = 1074918 mem = 536886519 peak = 1001660416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277811, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 379, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (394.42, 165.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670650 sequences (100000248 bp) in 2.171 CPU sec, 2.306 real sec...
[M::process] read 670702 sequences (100000122 bp) in 1.350 CPU sec, 1.352 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277757, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 376, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (392.71, 165.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670618 reads in 137.211 CPU sec, 4.361 real sec
[M::mem_process_seqs] Processed 670624 reads in 85.842 CPU sec, 2.722 real sec
sorting block 28, #reads = 1073919 mem = 536886709 peak = 1001660416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278148, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 377, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (393.56, 166.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670814 sequences (100000098 bp) in 1.884 CPU sec, 2.007 real sec...
[M::process] read 670898 sequences (100000110 bp) in 1.350 CPU sec, 1.354 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278481, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 379, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (395.08, 167.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670650 reads in 135.585 CPU sec, 4.321 real sec
sorting block 29, #reads = 1073784 mem = 536879729 peak = 1001660416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278323, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 377, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (394.08, 167.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670854 sequences (100000158 bp) in 1.537 CPU sec, 1.544 real sec...
[M::mem_process_seqs] Processed 670814 reads in 79.460 CPU sec, 2.723 real sec
[M::mem_process_seqs] Processed 670702 reads in 187.136 CPU sec, 6.151 real sec
sorting block 30, #reads = 1074017 mem = 536876096 peak = 1001660416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278063, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 377, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (393.93, 167.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670580 sequences (100000153 bp) in 1.935 CPU sec, 2.094 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 278517, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 379, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (395.15, 168.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670898 reads in 147.458 CPU sec, 4.684 real sec
[M::process] read 670492 sequences (100000082 bp) in 1.395 CPU sec, 1.403 real sec...
[M::mem_process_seqs] Processed 670854 reads in 85.430 CPU sec, 2.699 real sec
sorting block 31, #reads = 1074486 mem = 536880472 peak = 1001660416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278714, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 382, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (398.79, 169.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670536 sequences (100000230 bp) in 2.123 CPU sec, 2.258 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277839, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 386, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (401.99, 171.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672600 sequences (100000042 bp) in 1.327 CPU sec, 1.333 real sec...
[M::mem_process_seqs] Processed 670580 reads in 137.676 CPU sec, 4.385 real sec
[M::mem_process_seqs] Processed 670492 reads in 86.481 CPU sec, 2.740 real sec
sorting block 32, #reads = 1073877 mem = 536879570 peak = 1001660416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278715, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 388, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (404.30, 172.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (428, 5346, 5446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15482)
[M::mem_pestat] mean and std.dev: (3508.00, 2489.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 20500)
[M::mem_pestat] skip orientation RR
[M::process] read 672780 sequences (100000199 bp) in 1.925 CPU sec, 2.045 real sec...
[M::process] read 672338 sequences (100000199 bp) in 1.360 CPU sec, 1.360 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278599, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 363, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (381.73, 167.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670536 reads in 141.007 CPU sec, 4.496 real sec
sorting block 33, #reads = 1072753 mem = 536880366 peak = 1001660416
[M::process] read 672454 sequences (100000246 bp) in 1.507 CPU sec, 1.514 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277634, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 364, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (381.62, 167.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672780 reads in 81.008 CPU sec, 2.790 real sec
sorting block 34, #reads = 1073163 mem = 536878627 peak = 1001660416
[M::mem_process_seqs] Processed 672600 reads in 191.918 CPU sec, 6.317 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277771, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 366, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (383.81, 167.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672228 sequences (100000020 bp) in 1.925 CPU sec, 2.060 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278541, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 368, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (385.15, 168.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672106 sequences (100000118 bp) in 1.350 CPU sec, 1.351 real sec...
[M::mem_process_seqs] Processed 672338 reads in 149.509 CPU sec, 4.752 real sec
sorting block 35, #reads = 1075421 mem = 536881256 peak = 1001660416
[M::mem_process_seqs] Processed 672454 reads in 84.878 CPU sec, 2.686 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278707, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 370, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (386.28, 167.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 36, #reads = 1075423 mem = 536877796 peak = 1001660416
[M::process] read 671784 sequences (100000022 bp) in 2.233 CPU sec, 2.408 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279047, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 374, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (390.16, 169.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671660 sequences (100000055 bp) in 1.360 CPU sec, 1.360 real sec...
[M::mem_process_seqs] Processed 672228 reads in 135.643 CPU sec, 4.309 real sec
[M::mem_process_seqs] Processed 672106 reads in 84.393 CPU sec, 2.674 real sec
sorting block 37, #reads = 1075428 mem = 536879369 peak = 1001660416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278280, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 378, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (393.28, 169.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671342 sequences (100000125 bp) in 1.957 CPU sec, 2.131 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278406, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 377, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (392.49, 169.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671284 sequences (100000289 bp) in 1.360 CPU sec, 1.356 real sec...
[M::mem_process_seqs] Processed 671784 reads in 136.087 CPU sec, 4.335 real sec
[M::mem_process_seqs] Processed 671660 reads in 85.472 CPU sec, 2.704 real sec
sorting block 38, #reads = 1075121 mem = 536882445 peak = 1001660416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278092, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 384, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (397.87, 170.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670880 sequences (100000069 bp) in 2.143 CPU sec, 2.303 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 278310, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 670812 sequences (100000127 bp) in 1.370 CPU sec, 1.369 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (265, 383, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (398.18, 171.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671342 reads in 140.952 CPU sec, 4.487 real sec
[M::mem_process_seqs] Processed 671284 reads in 90.143 CPU sec, 2.860 real sec
sorting block 39, #reads = 1074951 mem = 536879196 peak = 1001660416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278098, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 389, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (401.80, 170.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670888 sequences (100000245 bp) in 2.132 CPU sec, 2.270 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277260, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 390, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (404.11, 172.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670760 sequences (100000139 bp) in 1.400 CPU sec, 1.394 real sec...
[M::mem_process_seqs] Processed 670880 reads in 144.832 CPU sec, 4.613 real sec
sorting block 40, #reads = 1075448 mem = 536882482 peak = 1001660416
[M::mem_process_seqs] Processed 670812 reads in 95.352 CPU sec, 3.044 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278096, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 390, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (403.49, 172.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 41, #reads = 1075477 mem = 536879077 peak = 1001660416
[M::process] read 670754 sequences (100000216 bp) in 2.663 CPU sec, 2.913 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277568, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 391, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (404.61, 171.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671216 sequences (100000274 bp) in 1.635 CPU sec, 1.639 real sec...
[M::mem_process_seqs] Processed 670888 reads in 160.921 CPU sec, 5.162 real sec
[M::mem_process_seqs] Processed 670760 reads in 103.006 CPU sec, 3.290 real sec
sorting block 42, #reads = 1074671 mem = 536879884 peak = 1001660416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277684, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 391, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (405.04, 172.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671114 sequences (100000054 bp) in 2.234 CPU sec, 2.376 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278364, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 373, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (389.60, 166.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671078 sequences (100000233 bp) in 1.590 CPU sec, 1.584 real sec...
[M::mem_process_seqs] Processed 670754 reads in 161.013 CPU sec, 5.167 real sec
[M::mem_process_seqs] Processed 671216 reads in 102.549 CPU sec, 3.265 real sec
sorting block 43, #reads = 1075095 mem = 536879462 peak = 1001660416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278279, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 376, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (392.77, 168.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670494 sequences (100000088 bp) in 2.443 CPU sec, 2.581 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277681, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 379, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (396.13, 170.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670510 sequences (100000042 bp) in 1.749 CPU sec, 1.743 real sec...
[M::mem_process_seqs] Processed 671114 reads in 164.362 CPU sec, 5.265 real sec
[M::mem_process_seqs] Processed 671078 reads in 106.955 CPU sec, 3.437 real sec
sorting block 44, #reads = 1074947 mem = 536877784 peak = 1001660416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278280, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 388, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (403.41, 171.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670596 sequences (100000055 bp) in 2.472 CPU sec, 2.667 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278573, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 386, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (401.78, 169.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670284 sequences (100000062 bp) in 1.864 CPU sec, 1.866 real sec...
[M::mem_process_seqs] Processed 670494 reads in 171.614 CPU sec, 5.481 real sec
sorting block 45, #reads = 1073812 mem = 536878064 peak = 1001660416
[M::mem_process_seqs] Processed 670510 reads in 109.880 CPU sec, 3.475 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277453, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 387, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (401.95, 170.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 46, #reads = 1074486 mem = 536880821 peak = 1001660416
[M::process] read 670558 sequences (100000197 bp) in 2.851 CPU sec, 3.019 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277725, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 388, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (403.68, 171.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670512 sequences (100000183 bp) in 1.666 CPU sec, 1.676 real sec...
[M::mem_process_seqs] Processed 670596 reads in 168.881 CPU sec, 5.380 real sec
[M::mem_process_seqs] Processed 670284 reads in 106.752 CPU sec, 3.378 real sec
sorting block 47, #reads = 1074386 mem = 536881748 peak = 1001660416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277997, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 388, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (403.56, 172.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670740 sequences (100000262 bp) in 2.388 CPU sec, 2.552 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 277451, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 385, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (400.75, 170.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670646 sequences (100000081 bp) in 1.840 CPU sec, 1.840 real sec...
[M::mem_process_seqs] Processed 670558 reads in 163.880 CPU sec, 5.211 real sec
[M::mem_process_seqs] Processed 670512 reads in 104.153 CPU sec, 3.288 real sec
sorting block 48, #reads = 1073763 mem = 536878311 peak = 1001660416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278590, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 385, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (400.99, 171.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670168 sequences (100000178 bp) in 2.781 CPU sec, 2.955 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278489, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 387, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (404.54, 174.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671686 sequences (100000011 bp) in 1.810 CPU sec, 1.808 real sec...
[M::mem_process_seqs] Processed 670740 reads in 160.880 CPU sec, 5.106 real sec
[M::mem_process_seqs] Processed 670646 reads in 103.533 CPU sec, 3.270 real sec
sorting block 49, #reads = 1073356 mem = 536879846 peak = 1001660416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277780, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (276, 398, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047)
[M::mem_pestat] mean and std.dev: (413.77, 177.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1304)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672476 sequences (100000210 bp) in 2.185 CPU sec, 2.317 real sec...
[M::process] read 672412 sequences (100000112 bp) in 1.540 CPU sec, 1.540 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 277860, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 375, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (393.80, 172.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670168 reads in 158.201 CPU sec, 5.046 real sec
sorting block 50, #reads = 1073136 mem = 536878764 peak = 1001660416
[M::mem_process_seqs] Processed 671686 reads in 98.687 CPU sec, 3.128 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 277240, 4, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (5571, 5571, 5571)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (5571, 5571)
[M::mem_pestat] mean and std.dev: (5571.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (5571, 5571)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 367, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (385.52, 169.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 51, #reads = 1073533 mem = 536880161 peak = 1001660416
[M::process] read 672202 sequences (100000189 bp) in 2.875 CPU sec, 3.024 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 276857, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 367, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (384.98, 168.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672246 sequences (100000287 bp) in 1.674 CPU sec, 1.676 real sec...
[M::mem_process_seqs] Processed 672476 reads in 161.840 CPU sec, 5.142 real sec
[M::mem_process_seqs] Processed 672412 reads in 103.768 CPU sec, 3.283 real sec
sorting block 52, #reads = 1077620 mem = 536880445 peak = 1001660416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277398, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 368, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (385.68, 168.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672258 sequences (100000075 bp) in 2.317 CPU sec, 2.507 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278272, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 370, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (387.08, 168.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671736 sequences (100000079 bp) in 1.770 CPU sec, 1.771 real sec...
[M::mem_process_seqs] Processed 672202 reads in 166.067 CPU sec, 5.306 real sec
[M::mem_process_seqs] Processed 672246 reads in 103.565 CPU sec, 3.272 real sec
sorting block 53, #reads = 1076775 mem = 536879545 peak = 1001660416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278090, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 370, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (388.20, 171.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671808 sequences (100000296 bp) in 2.671 CPU sec, 2.968 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277363, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 375, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (391.51, 170.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671774 sequences (100000247 bp) in 1.923 CPU sec, 1.933 real sec...
[M::mem_process_seqs] Processed 672258 reads in 172.573 CPU sec, 5.556 real sec
[M::mem_process_seqs] Processed 671736 reads in 113.189 CPU sec, 3.631 real sec
sorting block 54, #reads = 1076668 mem = 536876914 peak = 1001660416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277846, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 377, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (392.74, 169.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671512 sequences (100000022 bp) in 2.444 CPU sec, 2.913 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277496, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 376, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (391.17, 168.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671808 reads in 175.705 CPU sec, 5.801 real sec
[M::process] read 671302 sequences (100000021 bp) in 1.855 CPU sec, 1.953 real sec...
sorting block 55, #reads = 1075217 mem = 536877501 peak = 1001660416
[M::mem_process_seqs] Processed 671774 reads in 114.524 CPU sec, 3.785 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278006, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 380, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (395.40, 170.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671118 sequences (100000136 bp) in 2.630 CPU sec, 3.022 real sec...
sorting block 56, #reads = 1074720 mem = 536880111 peak = 1065250816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278048, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 384, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (399.74, 172.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671512 reads in 176.788 CPU sec, 5.867 real sec
[M::mem_process_seqs] Processed 671302 reads in 113.036 CPU sec, 3.745 real sec
[M::process] read 671096 sequences (100000199 bp) in 2.064 CPU sec, 2.322 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277720, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 383, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (398.92, 171.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 57, #reads = 1076129 mem = 536879997 peak = 1065250816
[M::process] read 671048 sequences (100000127 bp) in 2.171 CPU sec, 2.654 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277401, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 388, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (403.18, 173.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671118 reads in 178.075 CPU sec, 5.845 real sec
[M::mem_process_seqs] Processed 671096 reads in 117.179 CPU sec, 3.802 real sec
[M::process] read 670882 sequences (100000263 bp) in 2.130 CPU sec, 2.447 real sec...
sorting block 58, #reads = 1076579 mem = 536879898 peak = 1065250816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278049, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 389, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (403.44, 173.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670834 sequences (100000067 bp) in 2.463 CPU sec, 3.072 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277706, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 390, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (404.19, 172.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671048 reads in 181.739 CPU sec, 5.905 real sec
[M::mem_process_seqs] Processed 670882 reads in 118.717 CPU sec, 3.817 real sec
[M::process] read 670882 sequences (100000127 bp) in 2.428 CPU sec, 2.575 real sec...
sorting block 59, #reads = 1074687 mem = 536878898 peak = 1065250816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277491, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 389, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (403.76, 172.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670804 sequences (100000002 bp) in 2.168 CPU sec, 2.536 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277828, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 387, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (401.22, 170.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7705, 7705, 7705)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (7705, 7705)
[M::mem_pestat] mean and std.dev: (7705.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (7705, 7705)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 670834 reads in 181.747 CPU sec, 5.935 real sec
[M::mem_process_seqs] Processed 670882 reads in 119.028 CPU sec, 3.811 real sec
sorting block 60, #reads = 1074868 mem = 536879473 peak = 1065250816
[M::process] read 670758 sequences (100000075 bp) in 2.254 CPU sec, 2.489 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277629, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 386, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (400.30, 169.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670558 sequences (100000262 bp) in 2.536 CPU sec, 3.043 real sec...
sorting block 61, #reads = 1074772 mem = 536887145 peak = 1065250816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277551, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 385, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (399.95, 168.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670804 reads in 182.395 CPU sec, 5.942 real sec
[M::mem_process_seqs] Processed 670758 reads in 118.061 CPU sec, 3.783 real sec
[M::process] read 670480 sequences (100000064 bp) in 2.414 CPU sec, 2.694 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 278046, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 389, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (401.96, 168.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 62, #reads = 1074479 mem = 536879625 peak = 1065250816
[M::process] read 670440 sequences (100000150 bp) in 2.307 CPU sec, 2.592 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278058, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 389, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (402.32, 168.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670558 reads in 179.899 CPU sec, 5.802 real sec
[M::process] read 670484 sequences (100000002 bp) in 1.905 CPU sec, 1.926 real sec...
[M::mem_process_seqs] Processed 670480 reads in 116.016 CPU sec, 3.689 real sec
sorting block 63, #reads = 1073879 mem = 536881660 peak = 1065250816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277565, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 387, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (400.01, 166.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670298 sequences (100000115 bp) in 2.552 CPU sec, 2.955 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 276954, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 388, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (401.86, 168.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670440 reads in 175.839 CPU sec, 5.689 real sec
[M::process] read 670524 sequences (100000107 bp) in 1.778 CPU sec, 1.821 real sec...
[M::mem_process_seqs] Processed 670484 reads in 113.620 CPU sec, 3.646 real sec
sorting block 64, #reads = 1074045 mem = 536879180 peak = 1065250816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277231, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 386, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (400.86, 168.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670408 sequences (100000178 bp) in 2.269 CPU sec, 2.591 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277866, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 383, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (397.69, 166.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670490 sequences (100000199 bp) in 1.650 CPU sec, 1.645 real sec...
[M::mem_process_seqs] Processed 670298 reads in 172.545 CPU sec, 5.572 real sec
sorting block 65, #reads = 1073544 mem = 536877836 peak = 1065250816
[M::mem_process_seqs] Processed 670524 reads in 112.360 CPU sec, 3.599 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278441, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 383, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (397.74, 167.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 66, #reads = 1073158 mem = 536880084 peak = 1084329984
[M::process] read 670426 sequences (100000180 bp) in 2.595 CPU sec, 3.006 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278482, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 382, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (397.46, 167.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670408 reads in 173.534 CPU sec, 5.612 real sec
[M::process] read 673940 sequences (100000206 bp) in 1.977 CPU sec, 1.992 real sec...
[M::mem_process_seqs] Processed 670490 reads in 112.910 CPU sec, 3.618 real sec
sorting block 67, #reads = 1073236 mem = 536882033 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278130, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 387, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (402.15, 169.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673718 sequences (100000227 bp) in 2.405 CPU sec, 2.876 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 279657, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 348, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (369.92, 166.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673650 sequences (100000207 bp) in 1.862 CPU sec, 1.882 real sec...
[M::mem_process_seqs] Processed 670426 reads in 180.927 CPU sec, 5.839 real sec
[M::mem_process_seqs] Processed 673940 reads in 121.678 CPU sec, 3.893 real sec
sorting block 68, #reads = 1072831 mem = 536881082 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279367, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 354, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (374.78, 168.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673374 sequences (100000230 bp) in 2.944 CPU sec, 3.098 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279685, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 356, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (375.84, 168.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672340 sequences (100000017 bp) in 1.910 CPU sec, 1.910 real sec...
[M::mem_process_seqs] Processed 673718 reads in 183.866 CPU sec, 5.833 real sec
[M::mem_process_seqs] Processed 673650 reads in 118.166 CPU sec, 3.737 real sec
sorting block 69, #reads = 1070444 mem = 536879841 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279415, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 360, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (378.84, 168.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672226 sequences (100000270 bp) in 2.343 CPU sec, 2.553 real sec...
[M::process] read 671982 sequences (100000016 bp) in 1.968 CPU sec, 1.977 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278999, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 371, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (387.39, 169.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673374 reads in 183.579 CPU sec, 5.847 real sec
sorting block 70, #reads = 1071284 mem = 536881399 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278446, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 374, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (390.33, 170.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671694 sequences (100000148 bp) in 2.125 CPU sec, 2.134 real sec...
[M::mem_process_seqs] Processed 672226 reads in 104.386 CPU sec, 3.535 real sec
sorting block 71, #reads = 1071392 mem = 536879584 peak = 1084329984
[M::mem_process_seqs] Processed 672340 reads in 261.530 CPU sec, 8.549 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279032, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 380, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (394.10, 171.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671666 sequences (100000195 bp) in 2.532 CPU sec, 2.701 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278632, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 385, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (398.01, 171.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671982 reads in 194.296 CPU sec, 6.185 real sec
[M::process] read 671496 sequences (100000107 bp) in 1.863 CPU sec, 1.928 real sec...
[M::mem_process_seqs] Processed 671694 reads in 115.663 CPU sec, 3.649 real sec
sorting block 72, #reads = 1072864 mem = 536880335 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278660, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 384, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (397.07, 170.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 73, #reads = 1071694 mem = 536876743 peak = 1084329984
[M::process] read 671130 sequences (100000110 bp) in 2.732 CPU sec, 2.935 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 279085, 14, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (251, 251, 5548)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16142)
[M::mem_pestat] mean and std.dev: (2362.00, 2601.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 21439)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 385, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (398.03, 170.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (442, 442, 1135)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2521)
[M::mem_pestat] mean and std.dev: (550.82, 219.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3214)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::process] read 671260 sequences (100000085 bp) in 1.949 CPU sec, 1.951 real sec...
[M::mem_process_seqs] Processed 671666 reads in 182.858 CPU sec, 5.835 real sec
[M::mem_process_seqs] Processed 671496 reads in 119.179 CPU sec, 3.776 real sec
sorting block 74, #reads = 1070214 mem = 536879363 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278679, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 394, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054)
[M::mem_pestat] mean and std.dev: (406.19, 173.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1316)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671324 sequences (100000179 bp) in 2.607 CPU sec, 2.766 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278754, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 392, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1049)
[M::mem_pestat] mean and std.dev: (403.94, 173.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1310)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671048 sequences (100000189 bp) in 2.005 CPU sec, 2.011 real sec...
[M::mem_process_seqs] Processed 671130 reads in 183.837 CPU sec, 5.867 real sec
[M::mem_process_seqs] Processed 671260 reads in 119.636 CPU sec, 3.786 real sec
sorting block 75, #reads = 1070974 mem = 536879536 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 278126, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 389, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045)
[M::mem_pestat] mean and std.dev: (402.25, 172.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1305)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671106 sequences (100000063 bp) in 2.374 CPU sec, 2.539 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278542, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 670848 sequences (100000243 bp) in 2.025 CPU sec, 2.026 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (264, 385, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (398.64, 169.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671324 reads in 190.796 CPU sec, 6.094 real sec
[M::mem_process_seqs] Processed 671048 reads in 125.690 CPU sec, 3.981 real sec
sorting block 76, #reads = 1071345 mem = 536880212 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278393, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 384, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (398.32, 169.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1280)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670932 sequences (100000276 bp) in 2.567 CPU sec, 2.724 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 278290, 5, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (379, 459, 5419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15499)
[M::mem_pestat] mean and std.dev: (2471.10, 2665.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 20539)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 388, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (401.25, 170.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 670718 sequences (100000207 bp) in 2.054 CPU sec, 2.057 real sec...
[M::mem_process_seqs] Processed 671106 reads in 190.450 CPU sec, 6.084 real sec
sorting block 77, #reads = 1071009 mem = 536879693 peak = 1084329984
[M::mem_process_seqs] Processed 670848 reads in 123.197 CPU sec, 3.912 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278285, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 385, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (399.83, 169.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 78, #reads = 1070782 mem = 536885619 peak = 1084329984
[M::process] read 671148 sequences (100000168 bp) in 3.023 CPU sec, 3.196 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278696, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 387, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (400.39, 170.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671300 sequences (100000030 bp) in 2.012 CPU sec, 2.016 real sec...
[M::mem_process_seqs] Processed 670932 reads in 190.262 CPU sec, 6.061 real sec
[M::mem_process_seqs] Processed 670718 reads in 122.108 CPU sec, 3.872 real sec
sorting block 79, #reads = 1071477 mem = 536878221 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278719, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 381, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (396.19, 168.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671318 sequences (100000218 bp) in 2.611 CPU sec, 2.815 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278237, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 381, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (395.37, 168.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671094 sequences (100000269 bp) in 2.174 CPU sec, 2.182 real sec...
[M::mem_process_seqs] Processed 671148 reads in 188.716 CPU sec, 6.027 real sec
[M::mem_process_seqs] Processed 671300 reads in 123.160 CPU sec, 3.898 real sec
sorting block 80, #reads = 1071168 mem = 536877251 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279000, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 380, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (395.32, 169.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671048 sequences (100000250 bp) in 2.826 CPU sec, 2.989 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278880, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 383, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (397.77, 169.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670980 sequences (100000259 bp) in 1.961 CPU sec, 1.963 real sec...
[M::mem_process_seqs] Processed 671318 reads in 187.189 CPU sec, 5.975 real sec
[M::mem_process_seqs] Processed 671094 reads in 122.238 CPU sec, 3.876 real sec
sorting block 81, #reads = 1069479 mem = 536878216 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278275, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 383, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (398.55, 170.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670950 sequences (100000055 bp) in 2.607 CPU sec, 2.782 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 278599, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 383, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (398.04, 169.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672642 sequences (100000279 bp) in 2.003 CPU sec, 2.012 real sec...
[M::mem_process_seqs] Processed 671048 reads in 184.482 CPU sec, 5.890 real sec
sorting block 82, #reads = 1070830 mem = 536878654 peak = 1084329984
[M::mem_process_seqs] Processed 670980 reads in 119.226 CPU sec, 3.777 real sec
sorting block 83, #reads = 1071021 mem = 536883086 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279329, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 385, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (399.88, 172.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675582 sequences (100000150 bp) in 2.940 CPU sec, 3.070 real sec...
[M::process] read 674606 sequences (100000043 bp) in 2.042 CPU sec, 2.050 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278802, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 363, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (381.13, 167.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670950 reads in 191.793 CPU sec, 6.092 real sec
[M::mem_process_seqs] Processed 672642 reads in 128.198 CPU sec, 4.059 real sec
sorting block 84, #reads = 1068959 mem = 536880866 peak = 1084329984
[M::process] read 674698 sequences (100000097 bp) in 2.840 CPU sec, 2.966 real sec...
[M::process] read 674198 sequences (100000105 bp) in 2.027 CPU sec, 2.028 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280743, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 345, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (363.08, 160.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674606 reads in 105.372 CPU sec, 3.323 real sec
sorting block 85, #reads = 1067721 mem = 536877946 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280923, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 332, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (352.66, 156.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279965, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 341, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (358.13, 155.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673702 sequences (100000217 bp) in 2.228 CPU sec, 2.232 real sec...
[M::mem_process_seqs] Processed 675582 reads in 298.463 CPU sec, 9.467 real sec
[M::mem_process_seqs] Processed 674698 reads in 145.218 CPU sec, 4.627 real sec
sorting block 86, #reads = 1066806 mem = 536881750 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 279970, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 348, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (363.34, 156.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673312 sequences (100000286 bp) in 3.000 CPU sec, 3.141 real sec...
[M::process] read 672644 sequences (100000087 bp) in 1.678 CPU sec, 1.686 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280758, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 355, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (369.04, 158.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674198 reads in 188.547 CPU sec, 5.985 real sec
[M::mem_process_seqs] Processed 673702 reads in 120.624 CPU sec, 3.833 real sec
sorting block 87, #reads = 1066838 mem = 536882173 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280078, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 360, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (374.48, 161.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672344 sequences (100000023 bp) in 2.810 CPU sec, 2.949 real sec...
[M::process] read 672206 sequences (100000029 bp) in 1.950 CPU sec, 1.951 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279428, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 362, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (376.84, 161.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673312 reads in 185.866 CPU sec, 5.888 real sec
[M::mem_process_seqs] Processed 672644 reads in 118.929 CPU sec, 3.764 real sec
sorting block 88, #reads = 1067662 mem = 536878713 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279440, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 366, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (379.93, 162.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672116 sequences (100000110 bp) in 2.776 CPU sec, 2.911 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279830, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 371, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (384.25, 163.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672012 sequences (100000006 bp) in 1.930 CPU sec, 1.935 real sec...
[M::mem_process_seqs] Processed 672344 reads in 179.501 CPU sec, 5.693 real sec
sorting block 89, #reads = 1068841 mem = 536879855 peak = 1084329984
[M::mem_process_seqs] Processed 672206 reads in 114.455 CPU sec, 3.616 real sec
sorting block 90, #reads = 1068930 mem = 536880804 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279020, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 373, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (387.20, 166.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671658 sequences (100000038 bp) in 2.545 CPU sec, 2.647 real sec...
[M::process] read 671638 sequences (100000243 bp) in 1.570 CPU sec, 1.568 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279563, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 377, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (391.86, 169.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672116 reads in 173.328 CPU sec, 5.501 real sec
[M::mem_process_seqs] Processed 672012 reads in 110.838 CPU sec, 3.528 real sec
sorting block 91, #reads = 1070158 mem = 536880793 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278598, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 379, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (392.92, 168.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (5746, 5914, 5914)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (5410, 6250)
[M::mem_pestat] mean and std.dev: (5858.00, 79.20)
[M::mem_pestat] low and high boundaries for proper pairs: (5242, 6418)
[M::mem_pestat] skip orientation RR
[M::process] read 671360 sequences (100000172 bp) in 2.682 CPU sec, 2.776 real sec...
[M::process] read 671226 sequences (100000161 bp) in 1.880 CPU sec, 1.884 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279270, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 375, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (388.97, 165.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671658 reads in 176.418 CPU sec, 5.589 real sec
[M::mem_process_seqs] Processed 671638 reads in 109.948 CPU sec, 3.482 real sec
sorting block 92, #reads = 1069522 mem = 536884077 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278866, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 382, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (395.38, 168.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671106 sequences (100000264 bp) in 2.512 CPU sec, 2.620 real sec...
[M::process] read 670926 sequences (100000231 bp) in 1.930 CPU sec, 1.932 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279397, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 383, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (398.09, 170.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671360 reads in 177.127 CPU sec, 5.618 real sec
[M::mem_process_seqs] Processed 671226 reads in 115.653 CPU sec, 3.670 real sec
sorting block 93, #reads = 1068973 mem = 536880703 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278574, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 384, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (399.14, 171.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670934 sequences (100000054 bp) in 2.754 CPU sec, 2.894 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278329, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 387, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042)
[M::mem_pestat] mean and std.dev: (402.58, 173.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1301)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670970 sequences (100000001 bp) in 1.896 CPU sec, 1.897 real sec...
[M::mem_process_seqs] Processed 671106 reads in 180.474 CPU sec, 5.745 real sec
sorting block 94, #reads = 1070045 mem = 536876042 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277952, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 385, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044)
[M::mem_pestat] mean and std.dev: (401.62, 173.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1304)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671006 sequences (100000130 bp) in 2.081 CPU sec, 2.089 real sec...
[M::mem_process_seqs] Processed 670934 reads in 105.102 CPU sec, 3.539 real sec
sorting block 95, #reads = 1070645 mem = 536877080 peak = 1084329984
[M::mem_process_seqs] Processed 670926 reads in 254.797 CPU sec, 8.304 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278813, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 389, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056)
[M::mem_pestat] mean and std.dev: (403.81, 175.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1320)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671296 sequences (100000224 bp) in 2.612 CPU sec, 2.777 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278629, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 389, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055)
[M::mem_pestat] mean and std.dev: (404.98, 175.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1318)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670970 reads in 200.407 CPU sec, 6.371 real sec
[M::process] read 670910 sequences (100000038 bp) in 2.039 CPU sec, 2.066 real sec...
sorting block 96, #reads = 1071356 mem = 536882554 peak = 1084329984
[M::mem_process_seqs] Processed 671006 reads in 119.560 CPU sec, 3.771 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279011, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 386, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050)
[M::mem_pestat] mean and std.dev: (402.83, 175.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1312)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 97, #reads = 1070849 mem = 536883883 peak = 1084329984
[M::process] read 670844 sequences (100000193 bp) in 2.488 CPU sec, 2.631 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278151, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 394, 537)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075)
[M::mem_pestat] mean and std.dev: (410.96, 180.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1344)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673764 sequences (100000062 bp) in 1.938 CPU sec, 1.939 real sec...
[M::mem_process_seqs] Processed 671296 reads in 179.644 CPU sec, 5.727 real sec
[M::mem_process_seqs] Processed 670910 reads in 117.539 CPU sec, 3.728 real sec
sorting block 98, #reads = 1069903 mem = 536881728 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278317, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 392, 535)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1071)
[M::mem_pestat] mean and std.dev: (409.91, 180.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1339)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674090 sequences (100000170 bp) in 2.452 CPU sec, 2.563 real sec...
[M::process] read 673844 sequences (100000094 bp) in 1.950 CPU sec, 1.948 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279875, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 353, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (374.43, 168.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670844 reads in 191.649 CPU sec, 6.099 real sec
[M::mem_process_seqs] Processed 673764 reads in 123.678 CPU sec, 3.931 real sec
sorting block 99, #reads = 1064733 mem = 536878009 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280181, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 351, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (373.04, 168.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673086 sequences (100000298 bp) in 2.449 CPU sec, 2.587 real sec...
[M::process] read 672788 sequences (100000217 bp) in 1.811 CPU sec, 1.814 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280306, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 352, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (372.68, 166.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674090 reads in 176.800 CPU sec, 5.627 real sec
[M::mem_process_seqs] Processed 673844 reads in 113.091 CPU sec, 3.582 real sec
sorting block 100, #reads = 1069413 mem = 536879505 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279346, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 361, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (381.76, 171.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672358 sequences (100000171 bp) in 2.956 CPU sec, 3.124 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279162, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 365, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (384.86, 172.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672068 sequences (100000099 bp) in 1.901 CPU sec, 1.903 real sec...
[M::mem_process_seqs] Processed 673086 reads in 172.578 CPU sec, 5.473 real sec
sorting block 101, #reads = 1072760 mem = 536877897 peak = 1084329984
[M::mem_process_seqs] Processed 672788 reads in 111.133 CPU sec, 3.506 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279252, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 368, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (386.08, 169.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 102, #reads = 1072623 mem = 536881828 peak = 1084329984
[M::process] read 672248 sequences (100000229 bp) in 2.589 CPU sec, 2.734 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278478, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 371, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (388.66, 169.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672108 sequences (100000150 bp) in 1.900 CPU sec, 1.906 real sec...
[M::mem_process_seqs] Processed 672358 reads in 170.088 CPU sec, 5.406 real sec
[M::mem_process_seqs] Processed 672068 reads in 108.012 CPU sec, 3.416 real sec
sorting block 103, #reads = 1072476 mem = 536879971 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278511, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 368, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (386.67, 170.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671950 sequences (100000265 bp) in 2.909 CPU sec, 3.055 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279283, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 371, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (389.02, 169.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672248 reads in 167.116 CPU sec, 5.311 real sec
[M::process] read 671924 sequences (100000079 bp) in 1.934 CPU sec, 1.943 real sec...
[M::mem_process_seqs] Processed 672108 reads in 106.534 CPU sec, 3.357 real sec
sorting block 104, #reads = 1073375 mem = 536878585 peak = 1084329984
[M::process] read 671690 sequences (100000278 bp) in 2.479 CPU sec, 2.610 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278102, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 370, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (385.98, 165.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671722 sequences (100000258 bp) in 1.890 CPU sec, 1.886 real sec...
[M::mem_process_seqs] Processed 671924 reads in 101.811 CPU sec, 3.210 real sec
sorting block 105, #reads = 1074114 mem = 536881820 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279208, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 372, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (387.29, 166.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279071, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 373, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (388.87, 167.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671950 reads in 265.652 CPU sec, 8.436 real sec
[M::process] read 671980 sequences (100000094 bp) in 2.114 CPU sec, 2.127 real sec...
[M::mem_process_seqs] Processed 671690 reads in 124.423 CPU sec, 3.956 real sec
sorting block 106, #reads = 1072101 mem = 536878771 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 278423, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 373, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (389.17, 168.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672334 sequences (100000047 bp) in 2.728 CPU sec, 2.887 real sec...
[M::process] read 672220 sequences (100000216 bp) in 1.491 CPU sec, 1.498 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278514, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 373, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (388.83, 168.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671722 reads in 174.822 CPU sec, 5.557 real sec
[M::mem_process_seqs] Processed 671980 reads in 109.823 CPU sec, 3.488 real sec
sorting block 107, #reads = 1071644 mem = 536877170 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279312, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 363, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (381.01, 166.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672280 sequences (100000076 bp) in 3.108 CPU sec, 3.292 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278479, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 366, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (384.38, 168.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671494 sequences (100000119 bp) in 1.866 CPU sec, 1.870 real sec...
[M::mem_process_seqs] Processed 672334 reads in 176.739 CPU sec, 5.596 real sec
sorting block 108, #reads = 1071934 mem = 536876725 peak = 1084329984
[M::mem_process_seqs] Processed 672220 reads in 114.765 CPU sec, 3.624 real sec
sorting block 109, #reads = 1071064 mem = 536878150 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279339, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 369, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (386.52, 168.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671404 sequences (100000258 bp) in 2.661 CPU sec, 2.751 real sec...
[M::process] read 671314 sequences (100000278 bp) in 1.890 CPU sec, 1.888 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 278274, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 383, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (399.72, 175.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1301)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672280 reads in 175.696 CPU sec, 5.567 real sec
[M::mem_process_seqs] Processed 671494 reads in 112.852 CPU sec, 3.571 real sec
sorting block 110, #reads = 1070785 mem = 536881362 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278295, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 384, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048)
[M::mem_pestat] mean and std.dev: (401.45, 176.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1310)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671240 sequences (100000101 bp) in 2.784 CPU sec, 2.940 real sec...
[M::process] read 670830 sequences (100000054 bp) in 1.890 CPU sec, 1.898 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278929, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 383, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (399.17, 173.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1291)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671404 reads in 173.712 CPU sec, 5.499 real sec
[M::mem_process_seqs] Processed 671314 reads in 111.146 CPU sec, 3.512 real sec
sorting block 111, #reads = 1070602 mem = 536878853 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278639, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 386, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (401.53, 174.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1300)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671298 sequences (100000102 bp) in 2.674 CPU sec, 2.823 real sec...
[M::process] read 671248 sequences (100000074 bp) in 1.700 CPU sec, 1.696 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279148, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 391, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051)
[M::mem_pestat] mean and std.dev: (406.87, 176.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1312)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671240 reads in 173.348 CPU sec, 5.502 real sec
[M::mem_process_seqs] Processed 670830 reads in 111.690 CPU sec, 3.542 real sec
sorting block 112, #reads = 1071568 mem = 536883044 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278705, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 385, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055)
[M::mem_pestat] mean and std.dev: (404.17, 179.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1319)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671354 sequences (100000295 bp) in 2.611 CPU sec, 2.717 real sec...
[M::process] read 675448 sequences (100000221 bp) in 1.820 CPU sec, 1.825 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278803, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 382, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1058)
[M::mem_pestat] mean and std.dev: (402.98, 181.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1324)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671298 reads in 182.797 CPU sec, 5.799 real sec
sorting block 113, #reads = 1070827 mem = 536879476 peak = 1084329984
[M::mem_process_seqs] Processed 671248 reads in 119.439 CPU sec, 3.786 real sec
sorting block 114, #reads = 1069236 mem = 536880054 peak = 1084329984
[M::process] read 675438 sequences (100000064 bp) in 2.418 CPU sec, 2.510 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278708, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 386, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055)
[M::mem_pestat] mean and std.dev: (404.22, 179.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1319)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675802 sequences (100000002 bp) in 1.590 CPU sec, 1.586 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280391, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 334, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (354.30, 157.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280426, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 335, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (354.96, 157.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671354 reads in 276.518 CPU sec, 8.836 real sec
[M::mem_process_seqs] Processed 675448 reads in 204.953 CPU sec, 6.565 real sec
[M::mem_process_seqs] Processed 675438 reads in 223.076 CPU sec, 7.161 real sec
sorting block 115, #reads = 1063109 mem = 536878967 peak = 1084329984
[M::process] read 675388 sequences (100000080 bp) in 2.839 CPU sec, 2.994 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280971, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 331, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (349.85, 153.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675002 sequences (100000292 bp) in 1.835 CPU sec, 1.874 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280862, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 337, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (355.10, 156.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675802 reads in 184.833 CPU sec, 5.876 real sec
sorting block 116, #reads = 1067218 mem = 536878968 peak = 1084329984
[M::process] read 674024 sequences (100000005 bp) in 1.655 CPU sec, 1.691 real sec...
[M::mem_process_seqs] Processed 675388 reads in 118.335 CPU sec, 3.732 real sec
sorting block 117, #reads = 1067071 mem = 536884611 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280725, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 339, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (355.21, 153.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673440 sequences (100000139 bp) in 2.528 CPU sec, 2.648 real sec...
[M::process] read 672842 sequences (100000010 bp) in 1.850 CPU sec, 1.848 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279317, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 347, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (362.49, 156.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675002 reads in 188.101 CPU sec, 6.167 real sec
[M::mem_process_seqs] Processed 674024 reads in 116.324 CPU sec, 3.683 real sec
sorting block 118, #reads = 1067049 mem = 536882439 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 279835, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 357, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (372.66, 160.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672658 sequences (100000127 bp) in 2.349 CPU sec, 2.478 real sec...
[M::process] read 672534 sequences (100000179 bp) in 1.890 CPU sec, 1.891 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279648, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 361, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (376.31, 161.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673440 reads in 177.499 CPU sec, 5.637 real sec
[M::mem_process_seqs] Processed 672842 reads in 113.806 CPU sec, 3.605 real sec
sorting block 119, #reads = 1067972 mem = 536882554 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279176, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 366, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (380.57, 163.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672708 sequences (100000223 bp) in 2.410 CPU sec, 2.509 real sec...
[M::process] read 672460 sequences (100000265 bp) in 1.880 CPU sec, 1.881 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278690, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 366, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (380.96, 164.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672658 reads in 180.122 CPU sec, 5.973 real sec
sorting block 120, #reads = 1069437 mem = 536877728 peak = 1084329984
[M::mem_process_seqs] Processed 672534 reads in 111.569 CPU sec, 3.542 real sec
sorting block 121, #reads = 1069657 mem = 536880707 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278707, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 369, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (384.33, 166.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (363, 5292, 6530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18864)
[M::mem_pestat] mean and std.dev: (3199.20, 2868.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 25031)
[M::mem_pestat] skip orientation RR
[M::process] read 672262 sequences (100000135 bp) in 2.641 CPU sec, 2.796 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 279169, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 372, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (387.54, 168.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671782 sequences (100000172 bp) in 2.110 CPU sec, 2.116 real sec...
[M::mem_process_seqs] Processed 672708 reads in 181.648 CPU sec, 5.760 real sec
[M::mem_process_seqs] Processed 672460 reads in 116.761 CPU sec, 3.690 real sec
sorting block 122, #reads = 1069551 mem = 536878186 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278054, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 374, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (388.14, 167.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671770 sequences (100000045 bp) in 2.651 CPU sec, 2.762 real sec...
[M::process] read 671456 sequences (100000042 bp) in 1.500 CPU sec, 1.500 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278292, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 379, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (392.05, 167.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672262 reads in 177.521 CPU sec, 5.635 real sec
[M::mem_process_seqs] Processed 671782 reads in 110.756 CPU sec, 3.526 real sec
sorting block 123, #reads = 1069819 mem = 536881664 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279234, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 376, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (389.23, 165.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (550, 550, 3000)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7900)
[M::mem_pestat] mean and std.dev: (1877.09, 1585.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10350)
[M::mem_pestat] skip orientation RR
[M::process] read 671360 sequences (100000057 bp) in 2.389 CPU sec, 2.484 real sec...
[M::process] read 671108 sequences (100000212 bp) in 1.870 CPU sec, 1.876 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278737, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 377, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (391.85, 167.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671770 reads in 176.693 CPU sec, 5.615 real sec
[M::mem_process_seqs] Processed 671456 reads in 113.501 CPU sec, 3.584 real sec
sorting block 124, #reads = 1070337 mem = 536878527 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278336, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 383, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (396.45, 169.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670948 sequences (100000240 bp) in 2.642 CPU sec, 2.733 real sec...
[M::process] read 671048 sequences (100000245 bp) in 1.880 CPU sec, 1.874 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278564, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 388, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (400.88, 170.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671360 reads in 175.141 CPU sec, 5.554 real sec
sorting block 125, #reads = 1071146 mem = 536880420 peak = 1084329984
[M::process] read 671170 sequences (100000151 bp) in 1.711 CPU sec, 1.714 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278424, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 388, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (400.94, 170.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670948 reads in 99.501 CPU sec, 3.242 real sec
sorting block 126, #reads = 1071474 mem = 536878039 peak = 1084329984
[M::mem_process_seqs] Processed 671108 reads in 230.874 CPU sec, 7.411 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277693, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 386, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (400.25, 171.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670904 sequences (100000170 bp) in 2.516 CPU sec, 2.619 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278503, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 381, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (395.57, 168.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 644776 sequences (96059933 bp) in 1.810 CPU sec, 1.810 real sec...
[M::mem_process_seqs] Processed 671048 reads in 192.529 CPU sec, 6.115 real sec
[M::mem_process_seqs] Processed 671170 reads in 113.214 CPU sec, 3.577 real sec
sorting block 127, #reads = 1071676 mem = 536878691 peak = 1084329984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278064, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 385, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (400.26, 171.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 267910, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 379, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (395.83, 170.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670904 reads in 161.179 CPU sec, 5.298 real sec
sorting block 128, #reads = 1070806 mem = 536878473 peak = 1117171712
[M::mem_process_seqs] Processed 644776 reads in 102.771 CPU sec, 3.325 real sec
sorting block 129, #reads = 1072599 mem = 536878727 peak = 1134297088
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 138995328
overall: 69887819776 mem 19759.675 user 463.653 sys 665.587 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_Kh-T1-TDNA-1_A23MHWWLT4_1.L006\tPU:L006\tSM:659_Kh-T1-TDNA-1_A23MHWWLT4_1\tLB:659_Kh-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_Kh-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_Kh-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 665.638 sec; CPU: 20223.331 sec
sorting block 130, #reads = 233818 mem = 117434698 peak = 1134297088
uniq header count 1
merging 131 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 8199612590
output reads: 139674028
bam_mem_sort: 131 calls 70.715 user 0.829 sys 72.040 real
bam_write: 131 calls 50.788 user 2.849 sys 66.865 real
execute: 1 calls 40.323 user 1.992 sys 723.170 real
merge_files: 1 calls 40.307 user 1.966 sys 57.388 real
parse_chunk: 2084 calls 88.504 user 20.827 sys 115.285 real
read_chunk: 5613 calls 47.814 user 40.042 sys 620.063 real
sort_block: 1 calls 31.993 user 39.399 sys 597.012 real
write_chunk: 3527 calls 39.877 user 1.524 sys 56.242 real
overall: 3179098112 mem 2172.351 user 81.077 sys 723.193 real
ls: cannot access '*.cram': No such file or directory