cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_253-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1919.834 CPU sec, 70.436 real sec [M::process] read 684820 sequences (100000116 bp) in 1.011 CPU sec, 1.021 real sec... [M::process] read 680946 sequences (100000248 bp) in 1.374 CPU sec, 1.429 real sec... [M::process] read 681906 sequences (100000017 bp) in 1.265 CPU sec, 1.305 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 279572, 21, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (271, 451, 1364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3550) [M::mem_pestat] mean and std.dev: (700.29, 645.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 311, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (331.53, 162.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (435, 706, 940) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1950) [M::mem_pestat] mean and std.dev: (637.47, 190.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2455) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 242, 1220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3330) [M::mem_pestat] mean and std.dev: (610.28, 760.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4385) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682146 sequences (100000012 bp) in 1.154 CPU sec, 1.170 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 277814, 12, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 315, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1126) [M::mem_pestat] mean and std.dev: (304.89, 151.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (218, 339, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971) [M::mem_pestat] mean and std.dev: (354.43, 168.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1222) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1291, 1335, 1716) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (441, 2566) [M::mem_pestat] mean and std.dev: (1361.38, 300.97) [M::mem_pestat] low and high boundaries for proper pairs: (16, 2991) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (253, 290, 606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1312) [M::mem_pestat] mean and std.dev: (404.06, 257.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1665) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 278079, 12, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (229, 368, 1246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3280) [M::mem_pestat] mean and std.dev: (416.80, 318.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4297) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 333, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964) [M::mem_pestat] mean and std.dev: (350.03, 168.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1214) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (212, 687, 1481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4019) [M::mem_pestat] mean and std.dev: (889.91, 1045.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5288) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 353, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (302.54, 102.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684820 reads in 269.054 CPU sec, 8.656 real sec [M::mem_process_seqs] Processed 680946 reads in 185.136 CPU sec, 5.970 real sec [M::mem_process_seqs] Processed 681906 reads in 204.998 CPU sec, 6.606 real sec sorting block 0, #reads = 1068387 mem = 536880888 peak = 600784896 [M::process] read 683544 sequences (100000191 bp) in 2.238 CPU sec, 2.361 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 277958, 15, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 408, 739) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1795) [M::mem_pestat] mean and std.dev: (486.96, 399.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2323) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 330, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962) [M::mem_pestat] mean and std.dev: (347.80, 168.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1212) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (755, 1042, 2414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5732) [M::mem_pestat] mean and std.dev: (1281.29, 984.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7391) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 286, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (290.72, 130.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1170) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683912 sequences (100000229 bp) in 1.726 CPU sec, 1.732 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 279103, 3, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 380, 966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2560) [M::mem_pestat] mean and std.dev: (462.00, 397.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3357) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 319, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (339.35, 166.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1193) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 343, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992) [M::mem_pestat] mean and std.dev: (318.26, 137.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1257) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683958 sequences (100000191 bp) in 1.648 CPU sec, 1.647 real sec... [M::mem_process_seqs] Processed 682146 reads in 168.352 CPU sec, 5.315 real sec sorting block 1, #reads = 1067513 mem = 536876576 peak = 816267264 [M::mem_process_seqs] Processed 683544 reads in 112.156 CPU sec, 3.542 real sec sorting block 2, #reads = 1069216 mem = 536877948 peak = 908046336 [M::process] read 683836 sequences (100000002 bp) in 1.608 CPU sec, 1.696 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 278951, 9, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 274, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040) [M::mem_pestat] mean and std.dev: (267.33, 127.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1329) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 318, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945) [M::mem_pestat] mean and std.dev: (338.89, 166.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1192) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 286, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997) [M::mem_pestat] mean and std.dev: (331.06, 168.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1259) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680434 sequences (100000245 bp) in 1.360 CPU sec, 1.356 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 278757, 11, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 375, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997) [M::mem_pestat] mean and std.dev: (366.23, 183.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1252) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 317, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942) [M::mem_pestat] mean and std.dev: (338.05, 165.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1188) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (163, 1707, 1707) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4795) [M::mem_pestat] mean and std.dev: (1036.09, 821.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6339) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (250, 272, 557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1171) [M::mem_pestat] mean and std.dev: (290.97, 139.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1478) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 278645, 11, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 232, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (255.62, 108.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 316, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (336.17, 164.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1179) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (646, 737, 2398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5902) [M::mem_pestat] mean and std.dev: (1369.64, 1064.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7654) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 325, 1242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3308) [M::mem_pestat] mean and std.dev: (597.76, 594.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4341) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683912 reads in 239.147 CPU sec, 7.646 real sec [M::mem_process_seqs] Processed 683836 reads in 180.191 CPU sec, 5.799 real sec [M::mem_process_seqs] Processed 683958 reads in 197.990 CPU sec, 6.383 real sec sorting block 3, #reads = 1070256 mem = 536878091 peak = 908046336 [M::process] read 681652 sequences (100000040 bp) in 2.347 CPU sec, 2.516 real sec... sorting block 4, #reads = 1070300 mem = 536880883 peak = 908046336 [M::process] read 683516 sequences (100000069 bp) in 1.784 CPU sec, 1.836 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 278171, 6, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 303, 1039) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2775) [M::mem_pestat] mean and std.dev: (395.24, 360.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 333, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975) [M::mem_pestat] mean and std.dev: (351.18, 170.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1229) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (266, 416, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (344.63, 170.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681652 reads in 95.288 CPU sec, 2.989 real sec [M::process] read 682824 sequences (100000123 bp) in 1.684 CPU sec, 1.689 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 277513, 7, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (279, 310, 574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1164) [M::mem_pestat] mean and std.dev: (416.19, 206.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (221, 344, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995) [M::mem_pestat] mean and std.dev: (360.39, 172.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1253) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 268, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1208) [M::mem_pestat] mean and std.dev: (369.57, 202.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1545) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 278137, 19, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (271, 336, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (312.91, 112.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 317, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (339.02, 166.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1193) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (242, 260, 3867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11117) [M::mem_pestat] mean and std.dev: (2064.47, 3107.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14742) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 409, 536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1136) [M::mem_pestat] mean and std.dev: (347.06, 174.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1436) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682688 sequences (100000019 bp) in 1.949 CPU sec, 1.959 real sec... [M::mem_process_seqs] Processed 680434 reads in 263.084 CPU sec, 8.341 real sec sorting block 5, #reads = 1068699 mem = 536880311 peak = 908046336 [M::mem_process_seqs] Processed 683516 reads in 131.783 CPU sec, 4.183 real sec sorting block 6, #reads = 1068722 mem = 536878920 peak = 908046336 [M::process] read 682776 sequences (100000031 bp) in 1.925 CPU sec, 2.039 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 277719, 12, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 286, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1235) [M::mem_pestat] mean and std.dev: (386.12, 289.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 322, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948) [M::mem_pestat] mean and std.dev: (341.87, 166.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1195) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (265, 348, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (243.90, 163.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1081) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 402, 799) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2029) [M::mem_pestat] mean and std.dev: (575.82, 487.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2644) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682566 sequences (100000005 bp) in 1.328 CPU sec, 1.321 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 278214, 16, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 379, 548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1258) [M::mem_pestat] mean and std.dev: (351.38, 232.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 324, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955) [M::mem_pestat] mean and std.dev: (343.11, 166.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1204) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (289, 297, 1451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3775) [M::mem_pestat] mean and std.dev: (862.31, 1139.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5421) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 404, 583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1271) [M::mem_pestat] mean and std.dev: (378.17, 194.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1615) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682824 reads in 162.518 CPU sec, 5.178 real sec [M::mem_process_seqs] Processed 682688 reads in 107.203 CPU sec, 3.439 real sec sorting block 7, #reads = 1069448 mem = 536877598 peak = 908046336 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 278407, 18, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 313, 839) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2191) [M::mem_pestat] mean and std.dev: (486.04, 363.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2867) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 321, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951) [M::mem_pestat] mean and std.dev: (341.74, 167.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1199) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (296, 600, 2124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5780) [M::mem_pestat] mean and std.dev: (1028.94, 894.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7608) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 320, 734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1784) [M::mem_pestat] mean and std.dev: (493.35, 397.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2309) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680848 sequences (100000006 bp) in 2.227 CPU sec, 2.360 real sec... [M::process] read 681142 sequences (100000015 bp) in 1.908 CPU sec, 1.908 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 278074, 9, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 260, 786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1932) [M::mem_pestat] mean and std.dev: (469.54, 425.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 320, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942) [M::mem_pestat] mean and std.dev: (340.58, 165.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1187) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (282, 928, 1553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4095) [M::mem_pestat] mean and std.dev: (1053.04, 947.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5366) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682776 reads in 172.552 CPU sec, 5.472 real sec [M::mem_process_seqs] Processed 682566 reads in 113.985 CPU sec, 3.624 real sec sorting block 8, #reads = 1069791 mem = 536876699 peak = 908046336 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 277279, 9, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 299, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (319.15, 131.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1150) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 331, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959) [M::mem_pestat] mean and std.dev: (349.56, 167.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1207) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (272, 402, 1013) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2495) [M::mem_pestat] mean and std.dev: (504.55, 369.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3236) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682622 sequences (100000029 bp) in 2.079 CPU sec, 2.192 real sec... [M::process] read 682098 sequences (100000223 bp) in 1.869 CPU sec, 1.867 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 277426, 20, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 357, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1233) [M::mem_pestat] mean and std.dev: (374.33, 268.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 329, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952) [M::mem_pestat] mean and std.dev: (347.40, 166.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1198) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (170, 698, 808) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2084) [M::mem_pestat] mean and std.dev: (475.95, 354.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2722) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 320, 612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1446) [M::mem_pestat] mean and std.dev: (356.78, 192.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1863) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680848 reads in 171.446 CPU sec, 5.444 real sec sorting block 9, #reads = 1067977 mem = 536879521 peak = 908046336 [M::mem_process_seqs] Processed 681142 reads in 111.702 CPU sec, 3.551 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 278078, 17, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 328, 591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1345) [M::mem_pestat] mean and std.dev: (331.44, 173.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 316, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (337.02, 163.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1166) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (665, 1488, 2447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6011) [M::mem_pestat] mean and std.dev: (1528.35, 1155.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7793) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 233, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (281.18, 130.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680890 sequences (100000112 bp) in 2.297 CPU sec, 2.431 real sec... [M::process] read 682248 sequences (100000277 bp) in 1.469 CPU sec, 1.467 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 277190, 16, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 230, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045) [M::mem_pestat] mean and std.dev: (278.12, 197.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1336) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 323, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947) [M::mem_pestat] mean and std.dev: (342.80, 166.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1193) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (359, 856, 1085) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2537) [M::mem_pestat] mean and std.dev: (601.33, 403.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3263) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (257, 423, 1379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3623) [M::mem_pestat] mean and std.dev: (577.49, 483.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4745) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682622 reads in 171.773 CPU sec, 5.465 real sec sorting block 10, #reads = 1068384 mem = 536879500 peak = 908046336 [M::mem_process_seqs] Processed 682098 reads in 111.198 CPU sec, 3.549 real sec sorting block 11, #reads = 1069441 mem = 536877626 peak = 919961600 [M::process] read 682020 sequences (100000057 bp) in 2.066 CPU sec, 2.183 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 277158, 8, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 272, 588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1438) [M::mem_pestat] mean and std.dev: (267.50, 165.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1863) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 330, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950) [M::mem_pestat] mean and std.dev: (347.84, 165.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1195) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (296, 403, 789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1775) [M::mem_pestat] mean and std.dev: (479.36, 286.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2268) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682086 sequences (100000206 bp) in 1.500 CPU sec, 1.502 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 277983, 21, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (253, 312, 547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1135) [M::mem_pestat] mean and std.dev: (323.31, 156.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1429) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 319, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931) [M::mem_pestat] mean and std.dev: (339.19, 163.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (269, 594, 1920) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5222) [M::mem_pestat] mean and std.dev: (949.00, 1029.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6873) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 389, 659) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1509) [M::mem_pestat] mean and std.dev: (389.45, 194.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1934) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 277999, 8, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (257, 333, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962) [M::mem_pestat] mean and std.dev: (331.59, 134.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1197) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 320, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (338.61, 162.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (314, 353, 647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1313) [M::mem_pestat] mean and std.dev: (474.05, 267.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1646) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680890 reads in 250.478 CPU sec, 8.010 real sec [M::mem_process_seqs] Processed 682248 reads in 189.296 CPU sec, 6.069 real sec [M::mem_process_seqs] Processed 682020 reads in 207.781 CPU sec, 6.678 real sec sorting block 12, #reads = 1067992 mem = 536880751 peak = 919961600 [M::process] read 680058 sequences (100000262 bp) in 2.467 CPU sec, 2.653 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 277925, 8, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 307, 647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1639) [M::mem_pestat] mean and std.dev: (331.28, 251.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2135) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 320, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932) [M::mem_pestat] mean and std.dev: (340.37, 163.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1173) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 370, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966) [M::mem_pestat] mean and std.dev: (351.64, 169.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1212) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 13, #reads = 1068506 mem = 536880254 peak = 919961600 [M::process] read 680880 sequences (100000067 bp) in 2.029 CPU sec, 2.168 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 277324, 13, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 309, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1176) [M::mem_pestat] mean and std.dev: (319.15, 183.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (219, 335, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948) [M::mem_pestat] mean and std.dev: (350.85, 165.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1191) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1021, 2408, 2408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5182) [M::mem_pestat] mean and std.dev: (1640.58, 1012.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6569) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 486, 1056) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2730) [M::mem_pestat] mean and std.dev: (774.03, 788.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3928) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682086 reads in 169.714 CPU sec, 5.418 real sec [M::mem_process_seqs] Processed 680058 reads in 112.713 CPU sec, 3.555 real sec [M::process] read 682232 sequences (100000011 bp) in 1.825 CPU sec, 1.898 real sec... sorting block 14, #reads = 1069316 mem = 536878931 peak = 919961600 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 277261, 16, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 484, 1179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3117) [M::mem_pestat] mean and std.dev: (595.62, 583.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4086) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 329, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (346.25, 164.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1179) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1156, 1710, 2766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5986) [M::mem_pestat] mean and std.dev: (1509.47, 1023.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7596) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (272, 352, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (348.46, 147.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682314 sequences (100000185 bp) in 2.300 CPU sec, 2.437 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 277358, 13, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 275, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (271.05, 117.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 317, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (337.52, 162.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (61, 370, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1249) [M::mem_pestat] mean and std.dev: (251.82, 185.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1645) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (255, 409, 939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2307) [M::mem_pestat] mean and std.dev: (492.43, 296.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2991) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680804 sequences (100000149 bp) in 1.780 CPU sec, 1.779 real sec... [M::mem_process_seqs] Processed 680880 reads in 166.050 CPU sec, 5.268 real sec [M::mem_process_seqs] Processed 682232 reads in 109.572 CPU sec, 3.468 real sec sorting block 15, #reads = 1067892 mem = 536881048 peak = 919961600 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 277305, 7, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 257, 541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1321) [M::mem_pestat] mean and std.dev: (296.03, 221.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1711) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 317, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (337.76, 163.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1163) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 404, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019) [M::mem_pestat] mean and std.dev: (359.62, 189.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1288) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681728 sequences (100000101 bp) in 2.258 CPU sec, 2.364 real sec... [M::process] read 681930 sequences (100000064 bp) in 1.480 CPU sec, 1.476 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 277640, 8, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 265, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013) [M::mem_pestat] mean and std.dev: (255.20, 162.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1291) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 328, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (346.62, 165.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1183) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 327, 515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1103) [M::mem_pestat] mean and std.dev: (353.36, 260.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1397) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682314 reads in 168.506 CPU sec, 5.362 real sec sorting block 16, #reads = 1067754 mem = 536878992 peak = 919961600 [M::mem_process_seqs] Processed 680804 reads in 109.082 CPU sec, 3.528 real sec sorting block 17, #reads = 1068613 mem = 536877403 peak = 919961600 [M::process] read 681672 sequences (100000038 bp) in 1.987 CPU sec, 2.095 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 277914, 12, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 490, 783) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1923) [M::mem_pestat] mean and std.dev: (401.23, 250.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 321, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (341.57, 164.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1177) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (577, 869, 1238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2560) [M::mem_pestat] mean and std.dev: (752.00, 432.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3221) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 255, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031) [M::mem_pestat] mean and std.dev: (319.26, 196.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1303) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680942 sequences (100000162 bp) in 1.430 CPU sec, 1.428 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 277776, 14, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 567, 2535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7231) [M::mem_pestat] mean and std.dev: (1292.61, 1489.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 323, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947) [M::mem_pestat] mean and std.dev: (343.79, 167.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1193) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (212, 865, 1167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3077) [M::mem_pestat] mean and std.dev: (505.55, 414.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4032) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 327, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962) [M::mem_pestat] mean and std.dev: (318.89, 179.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1217) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 278093, 5, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (227, 334, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (290.04, 105.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 325, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949) [M::mem_pestat] mean and std.dev: (345.29, 167.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1195) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (297, 539, 1062) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2592) [M::mem_pestat] mean and std.dev: (574.08, 397.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3357) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681728 reads in 245.768 CPU sec, 7.922 real sec [M::mem_process_seqs] Processed 681930 reads in 182.677 CPU sec, 5.857 real sec [M::mem_process_seqs] Processed 681672 reads in 201.566 CPU sec, 6.485 real sec sorting block 18, #reads = 1069020 mem = 536879714 peak = 919961600 [M::process] read 682108 sequences (100000189 bp) in 2.296 CPU sec, 2.453 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 278084, 19, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (275, 296, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019) [M::mem_pestat] mean and std.dev: (333.98, 175.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1267) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 331, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965) [M::mem_pestat] mean and std.dev: (351.46, 170.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1215) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (748, 1632, 2059) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4681) [M::mem_pestat] mean and std.dev: (1525.37, 994.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5992) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (339, 528, 966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2220) [M::mem_pestat] mean and std.dev: (523.84, 311.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2847) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683242 sequences (100000086 bp) in 1.979 CPU sec, 2.075 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 277886, 8, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 323, 684) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1614) [M::mem_pestat] mean and std.dev: (346.38, 223.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2079) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 327, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949) [M::mem_pestat] mean and std.dev: (345.37, 167.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1195) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 289, 823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2025) [M::mem_pestat] mean and std.dev: (368.34, 316.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2626) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680942 reads in 172.435 CPU sec, 5.500 real sec sorting block 19, #reads = 1067745 mem = 536880202 peak = 919961600 [M::mem_process_seqs] Processed 682108 reads in 115.877 CPU sec, 3.649 real sec [M::process] read 682972 sequences (100000054 bp) in 1.953 CPU sec, 2.008 real sec... sorting block 20, #reads = 1067993 mem = 536879273 peak = 919961600 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 278241, 13, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 216, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (225.19, 117.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 321, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (340.49, 166.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1183) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (70, 313, 750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2110) [M::mem_pestat] mean and std.dev: (375.54, 336.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2790) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 417, 1004) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2694) [M::mem_pestat] mean and std.dev: (527.68, 427.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3539) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681834 sequences (100000249 bp) in 2.306 CPU sec, 2.432 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 277865, 14, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 272, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (245.46, 113.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 323, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937) [M::mem_pestat] mean and std.dev: (341.38, 165.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1180) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (117, 547, 547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1407) [M::mem_pestat] mean and std.dev: (356.00, 242.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1837) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 272, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (296.38, 155.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1083) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682976 sequences (100000135 bp) in 1.800 CPU sec, 1.796 real sec... [M::mem_process_seqs] Processed 683242 reads in 165.358 CPU sec, 5.257 real sec [M::mem_process_seqs] Processed 682972 reads in 110.456 CPU sec, 3.503 real sec sorting block 21, #reads = 1071067 mem = 536879579 peak = 919961600 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 277724, 12, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 292, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (286.44, 168.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 334, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967) [M::mem_pestat] mean and std.dev: (351.29, 169.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1218) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (721, 1209, 4727) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12739) [M::mem_pestat] mean and std.dev: (2238.75, 2176.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16745) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 218, 578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1406) [M::mem_pestat] mean and std.dev: (239.53, 119.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1820) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682846 sequences (100000208 bp) in 2.093 CPU sec, 2.221 real sec... [M::process] read 682548 sequences (100000196 bp) in 1.500 CPU sec, 1.495 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 278435, 10, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 243, 596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1488) [M::mem_pestat] mean and std.dev: (284.39, 171.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1934) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 325, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950) [M::mem_pestat] mean and std.dev: (343.76, 167.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1197) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (66, 351, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921) [M::mem_pestat] mean and std.dev: (180.78, 153.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1206) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (289, 321, 594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1204) [M::mem_pestat] mean and std.dev: (355.71, 167.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1509) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681834 reads in 165.715 CPU sec, 5.469 real sec [M::mem_process_seqs] Processed 682976 reads in 105.321 CPU sec, 3.357 real sec sorting block 22, #reads = 1069111 mem = 536879421 peak = 919961600 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278552, 12, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 382, 623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1495) [M::mem_pestat] mean and std.dev: (454.95, 341.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1931) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 327, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963) [M::mem_pestat] mean and std.dev: (345.84, 168.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1214) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (452, 5438, 5438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15410) [M::mem_pestat] mean and std.dev: (3369.58, 2452.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20396) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 300, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953) [M::mem_pestat] mean and std.dev: (343.63, 140.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1190) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682406 sequences (100000236 bp) in 2.186 CPU sec, 2.290 real sec... [M::process] read 682676 sequences (100000063 bp) in 1.422 CPU sec, 1.424 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 278203, 14, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (222, 264, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1137) [M::mem_pestat] mean and std.dev: (335.90, 163.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 329, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961) [M::mem_pestat] mean and std.dev: (347.33, 169.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1211) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (914, 1466, 1821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3635) [M::mem_pestat] mean and std.dev: (1447.07, 729.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4542) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (253, 370, 1030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2584) [M::mem_pestat] mean and std.dev: (605.43, 563.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3361) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682846 reads in 156.365 CPU sec, 4.977 real sec sorting block 23, #reads = 1071036 mem = 536879900 peak = 919961600 [M::mem_process_seqs] Processed 682548 reads in 102.782 CPU sec, 3.267 real sec sorting block 24, #reads = 1071395 mem = 536885165 peak = 919961600 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 278165, 12, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 368, 1091) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2839) [M::mem_pestat] mean and std.dev: (381.91, 252.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 326, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945) [M::mem_pestat] mean and std.dev: (344.11, 166.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1190) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (444, 613, 717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1263) [M::mem_pestat] mean and std.dev: (512.10, 147.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1536) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 305, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997) [M::mem_pestat] mean and std.dev: (308.97, 142.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1254) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681130 sequences (100000246 bp) in 2.108 CPU sec, 2.216 real sec... [M::process] read 681788 sequences (100000241 bp) in 1.389 CPU sec, 1.391 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 278101, 19, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 279, 703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1789) [M::mem_pestat] mean and std.dev: (445.60, 378.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2332) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 323, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (342.45, 166.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1192) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (510, 1252, 4388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12144) [M::mem_pestat] mean and std.dev: (2279.00, 2224.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16022) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (247, 422, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (336.43, 124.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682406 reads in 158.914 CPU sec, 5.268 real sec [M::mem_process_seqs] Processed 682676 reads in 103.272 CPU sec, 3.295 real sec sorting block 25, #reads = 1070449 mem = 536882570 peak = 919961600 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 277756, 10, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 200, 655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1629) [M::mem_pestat] mean and std.dev: (233.55, 155.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2116) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (217, 337, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967) [M::mem_pestat] mean and std.dev: (353.20, 169.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1217) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (241, 447, 1270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3328) [M::mem_pestat] mean and std.dev: (606.90, 459.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4357) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (256, 425, 626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1366) [M::mem_pestat] mean and std.dev: (384.05, 175.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1736) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682572 sequences (100000039 bp) in 2.127 CPU sec, 2.246 real sec... [M::process] read 682344 sequences (100000004 bp) in 1.390 CPU sec, 1.391 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 278159, 13, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (248, 379, 1327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3485) [M::mem_pestat] mean and std.dev: (695.32, 662.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 331, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958) [M::mem_pestat] mean and std.dev: (349.08, 168.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1206) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 15, 4766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14268) [M::mem_pestat] mean and std.dev: (1778.23, 2110.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19019) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 407, 567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1223) [M::mem_pestat] mean and std.dev: (394.48, 220.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1551) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681130 reads in 156.902 CPU sec, 4.997 real sec [M::mem_process_seqs] Processed 681788 reads in 104.229 CPU sec, 3.319 real sec sorting block 26, #reads = 1070644 mem = 536879388 peak = 919961600 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 278118, 11, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 296, 1202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3268) [M::mem_pestat] mean and std.dev: (726.23, 881.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4301) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 325, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948) [M::mem_pestat] mean and std.dev: (344.08, 167.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1194) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (644, 644, 936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (60, 1520) [M::mem_pestat] mean and std.dev: (667.40, 258.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1812) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 366, 745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1839) [M::mem_pestat] mean and std.dev: (383.67, 309.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2386) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682390 sequences (100000124 bp) in 2.161 CPU sec, 2.278 real sec... [M::process] read 682350 sequences (100000028 bp) in 1.540 CPU sec, 1.538 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278649, 22, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 511, 1325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3643) [M::mem_pestat] mean and std.dev: (696.53, 664.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4802) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 327, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958) [M::mem_pestat] mean and std.dev: (346.07, 168.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1207) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (114, 1070, 1493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4251) [M::mem_pestat] mean and std.dev: (1054.64, 897.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5630) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 380, 1036) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2648) [M::mem_pestat] mean and std.dev: (636.06, 588.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3454) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682572 reads in 158.244 CPU sec, 5.118 real sec [M::mem_process_seqs] Processed 682344 reads in 103.364 CPU sec, 3.285 real sec sorting block 27, #reads = 1070809 mem = 536884495 peak = 919961600 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 278681, 11, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 414, 1498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4208) [M::mem_pestat] mean and std.dev: (700.11, 673.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 324, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947) [M::mem_pestat] mean and std.dev: (343.15, 166.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1193) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (374, 702, 712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1388) [M::mem_pestat] mean and std.dev: (619.91, 360.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2063) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 397, 588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1336) [M::mem_pestat] mean and std.dev: (360.26, 200.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1710) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682078 sequences (100000019 bp) in 2.321 CPU sec, 2.430 real sec... [M::process] read 680342 sequences (100000108 bp) in 1.388 CPU sec, 1.405 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278259, 23, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (350, 450, 586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1058) [M::mem_pestat] mean and std.dev: (399.83, 148.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1294) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 325, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945) [M::mem_pestat] mean and std.dev: (344.16, 167.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1190) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (470, 1108, 1716) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4208) [M::mem_pestat] mean and std.dev: (1074.00, 630.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5454) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 493, 882) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2166) [M::mem_pestat] mean and std.dev: (577.88, 493.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2808) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682390 reads in 160.737 CPU sec, 5.123 real sec sorting block 28, #reads = 1071663 mem = 536879653 peak = 919961600 [M::mem_process_seqs] Processed 682350 reads in 110.951 CPU sec, 3.551 real sec sorting block 29, #reads = 1071267 mem = 536879489 peak = 924942336 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 278566, 12, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 297, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023) [M::mem_pestat] mean and std.dev: (332.77, 163.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1288) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 327, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953) [M::mem_pestat] mean and std.dev: (346.22, 168.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1200) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (70, 138, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1210) [M::mem_pestat] mean and std.dev: (239.58, 192.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1590) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (244, 297, 2072) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5728) [M::mem_pestat] mean and std.dev: (946.46, 1139.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7556) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 679960 sequences (100000041 bp) in 2.229 CPU sec, 2.309 real sec... [M::process] read 682296 sequences (100000228 bp) in 1.429 CPU sec, 1.428 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 277565, 10, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 245, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (262.03, 161.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (219, 337, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957) [M::mem_pestat] mean and std.dev: (353.09, 167.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1203) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (388, 1843, 9186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 26782) [M::mem_pestat] mean and std.dev: (3391.80, 3847.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 35580) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 374, 648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1608) [M::mem_pestat] mean and std.dev: (379.36, 218.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2088) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682078 reads in 158.151 CPU sec, 5.035 real sec [M::mem_process_seqs] Processed 680342 reads in 102.090 CPU sec, 3.240 real sec sorting block 30, #reads = 1069886 mem = 536876603 peak = 924942336 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 276887, 10, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 232, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (279.36, 122.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (221, 339, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965) [M::mem_pestat] mean and std.dev: (355.98, 169.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1213) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (443, 847, 1053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2273) [M::mem_pestat] mean and std.dev: (684.89, 311.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2883) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 219, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 494) [M::mem_pestat] mean and std.dev: (243.26, 73.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682376 sequences (100000088 bp) in 2.631 CPU sec, 2.731 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 278224, 5, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 256, 558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1324) [M::mem_pestat] mean and std.dev: (293.56, 173.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 322, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (341.76, 165.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 412, 864) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2114) [M::mem_pestat] mean and std.dev: (509.97, 409.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2739) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681790 sequences (100000023 bp) in 1.820 CPU sec, 1.822 real sec... [M::mem_process_seqs] Processed 679960 reads in 169.326 CPU sec, 5.375 real sec [M::mem_process_seqs] Processed 682296 reads in 117.163 CPU sec, 3.710 real sec sorting block 31, #reads = 1068169 mem = 536879714 peak = 924942336 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 277965, 24, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 273, 700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1794) [M::mem_pestat] mean and std.dev: (389.24, 360.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2341) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 322, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (342.69, 167.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1189) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (279, 3333, 6725) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 19617) [M::mem_pestat] mean and std.dev: (3766.25, 3691.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 26063) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (364, 382, 1230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2962) [M::mem_pestat] mean and std.dev: (582.59, 399.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3828) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682318 sequences (100000008 bp) in 2.153 CPU sec, 2.262 real sec... [M::process] read 682172 sequences (100000185 bp) in 1.798 CPU sec, 1.801 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278060, 13, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 230, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047) [M::mem_pestat] mean and std.dev: (286.75, 141.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1333) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 325, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954) [M::mem_pestat] mean and std.dev: (346.01, 169.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1202) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (485, 720, 2151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5483) [M::mem_pestat] mean and std.dev: (1001.69, 679.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7149) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 280, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048) [M::mem_pestat] mean and std.dev: (320.29, 171.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1328) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682376 reads in 170.117 CPU sec, 5.410 real sec sorting block 32, #reads = 1070099 mem = 536878935 peak = 924942336 [M::mem_process_seqs] Processed 681790 reads in 112.026 CPU sec, 3.555 real sec sorting block 33, #reads = 1070320 mem = 536878209 peak = 924942336 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 278001, 16, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 378, 512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062) [M::mem_pestat] mean and std.dev: (329.77, 168.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1337) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 322, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949) [M::mem_pestat] mean and std.dev: (342.93, 167.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1196) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (486, 806, 845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1563) [M::mem_pestat] mean and std.dev: (638.67, 299.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1922) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (278, 331, 739) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1661) [M::mem_pestat] mean and std.dev: (341.73, 148.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2122) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682406 sequences (100000108 bp) in 2.148 CPU sec, 2.248 real sec... [M::process] read 680364 sequences (100000050 bp) in 1.369 CPU sec, 1.374 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 278672, 9, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (266, 398, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953) [M::mem_pestat] mean and std.dev: (376.03, 174.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1182) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 322, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (343.37, 167.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1189) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 330, 561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1311) [M::mem_pestat] mean and std.dev: (302.79, 145.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1686) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682318 reads in 164.783 CPU sec, 5.255 real sec [M::mem_process_seqs] Processed 682172 reads in 107.686 CPU sec, 3.429 real sec sorting block 34, #reads = 1070131 mem = 536880548 peak = 924942336 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 278053, 8, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 189, 844) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2204) [M::mem_pestat] mean and std.dev: (374.74, 372.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2884) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 318, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (337.99, 163.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 301, 596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1306) [M::mem_pestat] mean and std.dev: (380.00, 269.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1661) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681604 sequences (100000105 bp) in 2.442 CPU sec, 2.570 real sec... [M::process] read 682504 sequences (100000121 bp) in 1.865 CPU sec, 1.864 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 277893, 19, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 252, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (267.52, 139.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1181) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (219, 336, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957) [M::mem_pestat] mean and std.dev: (353.20, 169.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1203) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (300, 632, 1484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3852) [M::mem_pestat] mean and std.dev: (578.56, 444.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5036) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 269, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016) [M::mem_pestat] mean and std.dev: (271.07, 125.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1295) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682406 reads in 170.970 CPU sec, 5.447 real sec [M::mem_process_seqs] Processed 680364 reads in 116.643 CPU sec, 3.713 real sec sorting block 35, #reads = 1070814 mem = 536881076 peak = 924942336 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 277673, 7, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 350, 593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1395) [M::mem_pestat] mean and std.dev: (382.30, 312.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1796) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 326, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (345.11, 166.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1188) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 315, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (265.34, 100.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682264 sequences (100000214 bp) in 2.451 CPU sec, 2.570 real sec... [M::process] read 682338 sequences (100000172 bp) in 1.835 CPU sec, 1.835 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 278413, 8, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 258, 796) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2036) [M::mem_pestat] mean and std.dev: (379.88, 352.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 320, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931) [M::mem_pestat] mean and std.dev: (340.19, 165.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 257, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1124) [M::mem_pestat] mean and std.dev: (311.80, 208.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1436) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681604 reads in 174.764 CPU sec, 5.560 real sec [M::mem_process_seqs] Processed 682504 reads in 117.301 CPU sec, 3.731 real sec sorting block 36, #reads = 1069370 mem = 536888420 peak = 924942336 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 278203, 11, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 267, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (252.82, 106.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 320, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931) [M::mem_pestat] mean and std.dev: (339.98, 165.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (274, 528, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036) [M::mem_pestat] mean and std.dev: (451.82, 245.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1433) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (254, 340, 540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1112) [M::mem_pestat] mean and std.dev: (345.90, 179.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1398) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682092 sequences (100000228 bp) in 2.506 CPU sec, 2.614 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 278367, 20, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 272, 574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1324) [M::mem_pestat] mean and std.dev: (370.32, 254.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 320, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931) [M::mem_pestat] mean and std.dev: (340.06, 165.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (237, 317, 1298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3420) [M::mem_pestat] mean and std.dev: (503.89, 540.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4481) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 282, 917) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2333) [M::mem_pestat] mean and std.dev: (530.61, 483.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3041) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682482 sequences (100000266 bp) in 1.826 CPU sec, 1.836 real sec... [M::mem_process_seqs] Processed 682264 reads in 171.151 CPU sec, 5.451 real sec sorting block 37, #reads = 1069451 mem = 536880921 peak = 924942336 [M::mem_process_seqs] Processed 682338 reads in 111.776 CPU sec, 3.549 real sec sorting block 38, #reads = 1068589 mem = 536880416 peak = 935325696 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 278445, 13, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 316, 719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1801) [M::mem_pestat] mean and std.dev: (372.03, 313.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2342) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 321, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (341.82, 166.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1181) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (357, 357, 816) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1734) [M::mem_pestat] mean and std.dev: (481.50, 294.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2193) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (253, 305, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (315.94, 177.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682404 sequences (100000217 bp) in 2.380 CPU sec, 2.543 real sec... [M::process] read 681990 sequences (100000013 bp) in 1.699 CPU sec, 1.745 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 278465, 14, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 247, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976) [M::mem_pestat] mean and std.dev: (263.07, 159.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1247) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 320, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945) [M::mem_pestat] mean and std.dev: (341.99, 168.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1191) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (906, 2819, 3304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8100) [M::mem_pestat] mean and std.dev: (2145.79, 1281.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10498) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 416, 757) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1787) [M::mem_pestat] mean and std.dev: (406.43, 223.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2302) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682092 reads in 179.208 CPU sec, 6.007 real sec [M::mem_process_seqs] Processed 682482 reads in 118.844 CPU sec, 4.129 real sec sorting block 39, #reads = 1069106 mem = 536877586 peak = 935325696 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 277847, 9, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 391, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062) [M::mem_pestat] mean and std.dev: (342.21, 179.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1356) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 322, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949) [M::mem_pestat] mean and std.dev: (343.29, 168.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1196) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 378, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (344.09, 149.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684674 sequences (100000157 bp) in 2.362 CPU sec, 2.639 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 278337, 10, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 378, 588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1312) [M::mem_pestat] mean and std.dev: (386.94, 207.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 322, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950) [M::mem_pestat] mean and std.dev: (344.34, 169.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1197) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1428, 2186, 2786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5502) [M::mem_pestat] mean and std.dev: (1855.60, 901.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6860) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 307, 621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1499) [M::mem_pestat] mean and std.dev: (407.36, 275.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1938) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684438 sequences (100000177 bp) in 1.696 CPU sec, 1.753 real sec... [M::mem_process_seqs] Processed 682404 reads in 178.174 CPU sec, 6.122 real sec [M::mem_process_seqs] Processed 681990 reads in 118.522 CPU sec, 4.093 real sec sorting block 40, #reads = 1067526 mem = 536878273 peak = 974413824 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 279391, 8, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (243.65, 139.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 312, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (330.36, 160.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 375, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (326.71, 139.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684570 sequences (100000282 bp) in 2.481 CPU sec, 2.797 real sec... [M::process] read 683692 sequences (100000073 bp) in 1.730 CPU sec, 1.783 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 278696, 8, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 551, 939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2375) [M::mem_pestat] mean and std.dev: (504.52, 431.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3093) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 314, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (331.00, 159.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 361, 842) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2120) [M::mem_pestat] mean and std.dev: (420.21, 281.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2759) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684674 reads in 186.787 CPU sec, 6.342 real sec sorting block 41, #reads = 1068908 mem = 536880559 peak = 974413824 [M::mem_process_seqs] Processed 684438 reads in 124.093 CPU sec, 4.211 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 279265, 17, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 272, 1533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4201) [M::mem_pestat] mean and std.dev: (723.81, 789.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 314, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (331.00, 159.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1150) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (203, 847, 1365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3689) [M::mem_pestat] mean and std.dev: (792.82, 546.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4851) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 317, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (7, 582) [M::mem_pestat] mean and std.dev: (284.96, 114.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683490 sequences (100000088 bp) in 2.463 CPU sec, 2.630 real sec... sorting block 42, #reads = 1069259 mem = 536878805 peak = 974413824 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 278529, 8, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 299, 1161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3065) [M::mem_pestat] mean and std.dev: (514.26, 520.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4017) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 317, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (334.64, 160.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (275, 328, 580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1190) [M::mem_pestat] mean and std.dev: (320.35, 112.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1495) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682656 sequences (100000073 bp) in 1.886 CPU sec, 1.920 real sec... [M::mem_process_seqs] Processed 684570 reads in 186.863 CPU sec, 6.606 real sec [M::mem_process_seqs] Processed 683692 reads in 124.081 CPU sec, 4.412 real sec sorting block 43, #reads = 1068754 mem = 536887871 peak = 974413824 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 278995, 9, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (387, 480, 703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1335) [M::mem_pestat] mean and std.dev: (500.07, 218.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 318, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (334.91, 160.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1150) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 211, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (56, 346) [M::mem_pestat] mean and std.dev: (199.36, 45.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 404) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681824 sequences (100000219 bp) in 2.603 CPU sec, 2.869 real sec... [M::process] read 680540 sequences (100000255 bp) in 1.888 CPU sec, 1.899 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278230, 16, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 441, 815) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2011) [M::mem_pestat] mean and std.dev: (357.71, 198.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 324, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936) [M::mem_pestat] mean and std.dev: (341.16, 163.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1178) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (322, 434, 867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1957) [M::mem_pestat] mean and std.dev: (501.71, 345.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2502) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 362, 688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1692) [M::mem_pestat] mean and std.dev: (470.20, 387.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2194) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683490 reads in 184.935 CPU sec, 6.526 real sec [M::mem_process_seqs] Processed 682656 reads in 127.098 CPU sec, 4.603 real sec sorting block 44, #reads = 1068726 mem = 536881157 peak = 974413824 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278246, 13, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 286, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000) [M::mem_pestat] mean and std.dev: (312.12, 168.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1279) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 335, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971) [M::mem_pestat] mean and std.dev: (351.03, 167.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1223) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (802, 1366, 1483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2845) [M::mem_pestat] mean and std.dev: (1001.69, 584.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3526) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 490, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1148) [M::mem_pestat] mean and std.dev: (396.95, 218.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1455) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683114 sequences (100000055 bp) in 2.464 CPU sec, 3.006 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 278495, 19, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (222, 356, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (334.94, 130.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (220, 340, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958) [M::mem_pestat] mean and std.dev: (353.48, 165.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1204) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (143, 1632, 5256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15482) [M::mem_pestat] mean and std.dev: (2487.95, 2219.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20595) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 387, 1062) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2804) [M::mem_pestat] mean and std.dev: (485.70, 346.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3675) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683344 sequences (100000010 bp) in 1.730 CPU sec, 1.828 real sec... [M::mem_process_seqs] Processed 681824 reads in 181.485 CPU sec, 7.041 real sec [M::mem_process_seqs] Processed 680540 reads in 119.650 CPU sec, 4.765 real sec sorting block 45, #reads = 1068362 mem = 536880861 peak = 974413824 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 278488, 16, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (250, 447, 955) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2365) [M::mem_pestat] mean and std.dev: (611.16, 601.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3070) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 320, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (338.20, 162.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (617, 804, 2542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6392) [M::mem_pestat] mean and std.dev: (1418.93, 1229.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8317) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (274, 433, 585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1207) [M::mem_pestat] mean and std.dev: (339.69, 162.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1518) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682846 sequences (100000282 bp) in 2.471 CPU sec, 2.851 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 278389, 11, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 391, 660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1596) [M::mem_pestat] mean and std.dev: (362.24, 241.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2064) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 320, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (338.55, 164.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1178) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (235, 811, 1333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3529) [M::mem_pestat] mean and std.dev: (577.56, 399.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4627) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 221, 739) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1893) [M::mem_pestat] mean and std.dev: (329.81, 308.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2470) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682818 sequences (100000104 bp) in 1.847 CPU sec, 1.926 real sec... [M::mem_process_seqs] Processed 683114 reads in 184.928 CPU sec, 6.753 real sec sorting block 46, #reads = 1069554 mem = 536877948 peak = 974413824 [M::mem_process_seqs] Processed 683344 reads in 123.379 CPU sec, 4.587 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 278620, 11, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 302, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954) [M::mem_pestat] mean and std.dev: (330.38, 196.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1207) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 323, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940) [M::mem_pestat] mean and std.dev: (340.81, 164.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1184) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (590, 797, 915) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1565) [M::mem_pestat] mean and std.dev: (637.80, 259.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1890) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (245, 297, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040) [M::mem_pestat] mean and std.dev: (289.90, 114.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1305) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 47, #reads = 1069986 mem = 536878342 peak = 1013035008 [M::process] read 682536 sequences (100000120 bp) in 2.370 CPU sec, 2.764 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 278343, 11, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (316, 417, 1407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3589) [M::mem_pestat] mean and std.dev: (730.36, 646.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 322, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (340.10, 163.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1176) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (81, 81, 804) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2250) [M::mem_pestat] mean and std.dev: (382.18, 557.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2973) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 422, 592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1376) [M::mem_pestat] mean and std.dev: (411.13, 187.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1768) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682392 sequences (100000084 bp) in 1.785 CPU sec, 2.202 real sec... [M::mem_process_seqs] Processed 682846 reads in 185.958 CPU sec, 7.229 real sec [M::mem_process_seqs] Processed 682818 reads in 124.353 CPU sec, 5.280 real sec sorting block 48, #reads = 1071150 mem = 536884297 peak = 1013035008 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 277945, 9, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 325, 556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1300) [M::mem_pestat] mean and std.dev: (313.47, 184.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 322, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (339.85, 162.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1169) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 283, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1183) [M::mem_pestat] mean and std.dev: (290.81, 137.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1512) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682686 sequences (100000124 bp) in 2.591 CPU sec, 2.844 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 277883, 11, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 529, 1046) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2772) [M::mem_pestat] mean and std.dev: (399.15, 275.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 324, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (341.30, 164.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1181) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (149, 1028, 1097) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2993) [M::mem_pestat] mean and std.dev: (681.00, 634.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3941) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (286, 534, 541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051) [M::mem_pestat] mean and std.dev: (404.03, 185.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1306) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681826 sequences (100000095 bp) in 1.873 CPU sec, 1.879 real sec... [M::mem_process_seqs] Processed 682536 reads in 178.644 CPU sec, 6.044 real sec [M::mem_process_seqs] Processed 682392 reads in 119.867 CPU sec, 3.970 real sec sorting block 49, #reads = 1070298 mem = 536877391 peak = 1013035008 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 278369, 7, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 376, 748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1882) [M::mem_pestat] mean and std.dev: (343.18, 264.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 324, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950) [M::mem_pestat] mean and std.dev: (343.34, 166.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1197) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 285, 648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1498) [M::mem_pestat] mean and std.dev: (342.60, 198.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1923) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680162 sequences (100000251 bp) in 2.375 CPU sec, 2.694 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 278021, 17, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (238, 322, 658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1498) [M::mem_pestat] mean and std.dev: (358.49, 223.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1918) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 327, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947) [M::mem_pestat] mean and std.dev: (345.13, 165.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1192) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (411, 785, 1374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3300) [M::mem_pestat] mean and std.dev: (785.00, 467.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4263) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (264, 341, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (373.58, 174.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681306 sequences (100000267 bp) in 1.591 CPU sec, 1.635 real sec... [M::mem_process_seqs] Processed 682686 reads in 180.459 CPU sec, 6.014 real sec sorting block 50, #reads = 1068048 mem = 536878918 peak = 1013035008 [M::mem_process_seqs] Processed 681826 reads in 117.485 CPU sec, 3.901 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 277258, 18, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 286, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (276.27, 107.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (222, 340, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954) [M::mem_pestat] mean and std.dev: (353.73, 165.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1198) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (246, 450, 755) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1773) [M::mem_pestat] mean and std.dev: (483.19, 353.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2282) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (251, 405, 1068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2702) [M::mem_pestat] mean and std.dev: (743.38, 720.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3624) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682102 sequences (100000299 bp) in 2.532 CPU sec, 2.781 real sec... sorting block 51, #reads = 1069339 mem = 536880528 peak = 1049366528 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 277893, 10, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 235, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (237.77, 106.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 332, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954) [M::mem_pestat] mean and std.dev: (348.86, 166.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1200) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (232, 412, 1714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4678) [M::mem_pestat] mean and std.dev: (828.70, 902.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6160) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (266, 535, 1012) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2504) [M::mem_pestat] mean and std.dev: (669.77, 540.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3250) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682188 sequences (100000293 bp) in 2.022 CPU sec, 2.333 real sec... [M::mem_process_seqs] Processed 680162 reads in 178.411 CPU sec, 6.449 real sec [M::mem_process_seqs] Processed 681306 reads in 120.226 CPU sec, 4.505 real sec sorting block 52, #reads = 1069080 mem = 536878128 peak = 1049366528 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 278152, 7, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 484, 1044) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2746) [M::mem_pestat] mean and std.dev: (514.33, 375.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 323, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (341.36, 163.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (251, 370, 1054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2660) [M::mem_pestat] mean and std.dev: (535.56, 451.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3463) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682152 sequences (100000120 bp) in 2.542 CPU sec, 2.894 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 277763, 8, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 319, 629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1539) [M::mem_pestat] mean and std.dev: (450.11, 388.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2005) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 323, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (342.33, 165.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1182) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 312, 705) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1743) [M::mem_pestat] mean and std.dev: (295.71, 151.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2262) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682266 sequences (100000027 bp) in 1.906 CPU sec, 2.201 real sec... [M::mem_process_seqs] Processed 682102 reads in 180.638 CPU sec, 6.366 real sec [M::mem_process_seqs] Processed 682188 reads in 122.654 CPU sec, 4.547 real sec sorting block 53, #reads = 1069614 mem = 536879230 peak = 1049366528 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278036, 12, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 446, 1018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2604) [M::mem_pestat] mean and std.dev: (647.75, 560.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3397) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 324, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945) [M::mem_pestat] mean and std.dev: (343.52, 166.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1190) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (125, 837, 1284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3602) [M::mem_pestat] mean and std.dev: (670.42, 498.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4761) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 346, 647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1495) [M::mem_pestat] mean and std.dev: (422.90, 289.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1919) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681702 sequences (100000226 bp) in 2.623 CPU sec, 3.126 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 277718, 28, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 205, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (216.84, 112.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 322, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (342.11, 165.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1185) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (322, 1176, 1282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3202) [M::mem_pestat] mean and std.dev: (875.12, 511.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4162) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 325, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1177) [M::mem_pestat] mean and std.dev: (323.62, 174.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1493) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682190 sequences (100000143 bp) in 1.789 CPU sec, 1.822 real sec... [M::mem_process_seqs] Processed 682152 reads in 183.392 CPU sec, 6.328 real sec [M::mem_process_seqs] Processed 682266 reads in 124.597 CPU sec, 4.171 real sec sorting block 54, #reads = 1070407 mem = 536879624 peak = 1049366528 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 277815, 25, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (256, 421, 1115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2833) [M::mem_pestat] mean and std.dev: (755.80, 773.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3849) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 322, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (341.69, 164.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (584, 759, 1240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2552) [M::mem_pestat] mean and std.dev: (1094.20, 781.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4219) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 274, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1080) [M::mem_pestat] mean and std.dev: (314.86, 178.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1371) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682176 sequences (100000142 bp) in 2.540 CPU sec, 3.093 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 277585, 13, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 290, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1123) [M::mem_pestat] mean and std.dev: (273.43, 140.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 319, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (338.13, 162.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (86, 434, 1302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3734) [M::mem_pestat] mean and std.dev: (592.15, 652.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4950) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (272, 363, 608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1280) [M::mem_pestat] mean and std.dev: (380.19, 193.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1616) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682566 sequences (100000287 bp) in 1.886 CPU sec, 1.926 real sec... [M::mem_process_seqs] Processed 681702 reads in 183.127 CPU sec, 6.262 real sec sorting block 55, #reads = 1069633 mem = 536879841 peak = 1049366528 [M::mem_process_seqs] Processed 682190 reads in 129.657 CPU sec, 4.425 real sec sorting block 56, #reads = 1069712 mem = 536878875 peak = 1057124352 [M::process] read 682394 sequences (100000224 bp) in 2.604 CPU sec, 3.050 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 277867, 7, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 342, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047) [M::mem_pestat] mean and std.dev: (337.85, 213.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1332) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 318, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (338.99, 164.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1171) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 352, 571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1339) [M::mem_pestat] mean and std.dev: (394.98, 257.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1723) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682566 reads in 104.609 CPU sec, 3.750 real sec [M::process] read 682192 sequences (100000037 bp) in 1.804 CPU sec, 2.027 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 277935, 10, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (293, 425, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (318.89, 135.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 320, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932) [M::mem_pestat] mean and std.dev: (340.60, 165.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1173) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (231, 280, 1764) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4830) [M::mem_pestat] mean and std.dev: (447.75, 514.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6363) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 313, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (50, 510) [M::mem_pestat] mean and std.dev: (278.12, 81.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 277494, 11, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 391, 772) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1846) [M::mem_pestat] mean and std.dev: (480.88, 327.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2383) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 319, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931) [M::mem_pestat] mean and std.dev: (339.49, 164.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 1033) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2873) [M::mem_pestat] mean and std.dev: (411.70, 392.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3793) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (118, 246, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952) [M::mem_pestat] mean and std.dev: (230.46, 121.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1230) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682176 reads in 282.106 CPU sec, 9.965 real sec sorting block 57, #reads = 1070894 mem = 536877742 peak = 1057124352 [M::process] read 681744 sequences (100000067 bp) in 2.172 CPU sec, 2.601 real sec... [M::mem_process_seqs] Processed 682394 reads in 141.575 CPU sec, 5.126 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 277584, 11, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 316, 933) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2373) [M::mem_pestat] mean and std.dev: (469.94, 418.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3093) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 320, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (339.65, 164.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1168) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (323, 1447, 4192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11930) [M::mem_pestat] mean and std.dev: (2348.82, 2779.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15799) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 499, 683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1635) [M::mem_pestat] mean and std.dev: (549.11, 412.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2198) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682532 sequences (100000209 bp) in 2.355 CPU sec, 2.919 real sec... sorting block 58, #reads = 1069514 mem = 536879907 peak = 1059610624 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 277877, 4, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 318, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1221) [M::mem_pestat] mean and std.dev: (319.00, 213.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 323, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (342.96, 166.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1182) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 261, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (299.36, 154.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1179) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682192 reads in 178.838 CPU sec, 6.438 real sec [M::process] read 681990 sequences (100000181 bp) in 1.950 CPU sec, 2.016 real sec... [M::mem_process_seqs] Processed 681744 reads in 120.231 CPU sec, 4.473 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 278355, 14, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 200, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (259.54, 147.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1019) [M::mem_pestat] analyzing insert size distribution for orientation FR... sorting block 59, #reads = 1070572 mem = 536881725 peak = 1059610624 [M::mem_pestat] (25, 50, 75) percentile: (207, 318, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936) [M::mem_pestat] mean and std.dev: (339.79, 166.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1179) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (582, 1491, 1877) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4467) [M::mem_pestat] mean and std.dev: (1426.15, 1083.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5762) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 303, 567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1315) [M::mem_pestat] mean and std.dev: (375.67, 226.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1689) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682416 sequences (100000035 bp) in 2.498 CPU sec, 2.970 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 278326, 9, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 351, 688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1682) [M::mem_pestat] mean and std.dev: (387.73, 244.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2179) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 323, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (343.01, 167.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1189) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 307, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (283.42, 140.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682532 reads in 178.256 CPU sec, 6.237 real sec [M::process] read 683164 sequences (100000006 bp) in 1.912 CPU sec, 2.029 real sec... [M::mem_process_seqs] Processed 681990 reads in 122.373 CPU sec, 4.391 real sec sorting block 60, #reads = 1070343 mem = 536878220 peak = 1059610624 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 277975, 8, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 322, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (290.70, 99.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 318, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (340.81, 167.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1183) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (267, 508, 954) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2328) [M::mem_pestat] mean and std.dev: (574.13, 411.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3015) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682730 sequences (100000023 bp) in 2.424 CPU sec, 3.005 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 277869, 15, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 352, 1113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2927) [M::mem_pestat] mean and std.dev: (461.00, 367.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3834) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 317, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931) [M::mem_pestat] mean and std.dev: (337.61, 165.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1173) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 203, 831) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2459) [M::mem_pestat] mean and std.dev: (376.62, 576.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3273) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 349, 4075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11743) [M::mem_pestat] mean and std.dev: (1457.06, 2047.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15577) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682416 reads in 182.315 CPU sec, 6.365 real sec [M::process] read 681528 sequences (100000112 bp) in 2.110 CPU sec, 2.685 real sec... [M::mem_process_seqs] Processed 683164 reads in 131.190 CPU sec, 4.401 real sec sorting block 61, #reads = 1069416 mem = 536877577 peak = 1059610624 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 277572, 13, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 279, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (243.38, 112.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1193) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 322, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (337.76, 160.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (602, 1707, 3332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8792) [M::mem_pestat] mean and std.dev: (1865.23, 1634.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11522) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 291, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007) [M::mem_pestat] mean and std.dev: (275.91, 129.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1292) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681364 sequences (100000013 bp) in 2.401 CPU sec, 2.532 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 277711, 9, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 375, 1225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3343) [M::mem_pestat] mean and std.dev: (748.92, 815.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 327, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (341.75, 160.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 260, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942) [M::mem_pestat] mean and std.dev: (288.44, 178.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1195) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681226 sequences (100000286 bp) in 1.765 CPU sec, 1.768 real sec... [M::mem_process_seqs] Processed 682730 reads in 175.994 CPU sec, 5.628 real sec sorting block 62, #reads = 1068651 mem = 536880230 peak = 1059610624 [M::mem_process_seqs] Processed 681528 reads in 120.410 CPU sec, 3.824 real sec sorting block 63, #reads = 1068486 mem = 536880826 peak = 1080606720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 277387, 12, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (319, 437, 705) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1477) [M::mem_pestat] mean and std.dev: (464.65, 230.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1863) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 330, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (344.21, 161.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1163) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (364, 659, 2136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5680) [M::mem_pestat] mean and std.dev: (706.40, 561.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7452) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 291, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1227) [M::mem_pestat] mean and std.dev: (293.40, 166.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1572) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682340 sequences (100000041 bp) in 2.570 CPU sec, 2.707 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 276588, 4, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 278, 578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1272) [M::mem_pestat] mean and std.dev: (365.28, 240.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 328, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (343.54, 161.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1163) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (261, 336, 673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1497) [M::mem_pestat] mean and std.dev: (427.63, 265.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1909) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682292 sequences (100000047 bp) in 1.849 CPU sec, 1.852 real sec... [M::mem_process_seqs] Processed 681364 reads in 173.822 CPU sec, 5.542 real sec [M::mem_process_seqs] Processed 681226 reads in 117.822 CPU sec, 3.737 real sec sorting block 64, #reads = 1069020 mem = 536881848 peak = 1080606720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 277444, 7, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 397, 625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1439) [M::mem_pestat] mean and std.dev: (396.28, 220.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1846) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 319, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (335.15, 158.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 611, 966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2488) [M::mem_pestat] mean and std.dev: (580.08, 477.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3249) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681170 sequences (100000298 bp) in 2.179 CPU sec, 2.303 real sec... [M::process] read 679882 sequences (100000089 bp) in 1.653 CPU sec, 1.662 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 278451, 17, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 204, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1090) [M::mem_pestat] mean and std.dev: (294.19, 201.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 322, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (338.17, 160.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1150) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (346, 515, 2412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6544) [M::mem_pestat] mean and std.dev: (1275.76, 1043.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8610) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (266, 343, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (22, 632) [M::mem_pestat] mean and std.dev: (308.65, 109.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682340 reads in 169.621 CPU sec, 5.414 real sec [M::mem_process_seqs] Processed 682292 reads in 115.785 CPU sec, 3.690 real sec sorting block 65, #reads = 1069432 mem = 536877192 peak = 1080606720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 277801, 8, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 402, 1215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3193) [M::mem_pestat] mean and std.dev: (660.54, 589.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4182) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (217, 333, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940) [M::mem_pestat] mean and std.dev: (347.76, 164.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1181) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (251, 327, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956) [M::mem_pestat] mean and std.dev: (362.60, 156.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1191) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682204 sequences (100000181 bp) in 2.407 CPU sec, 2.575 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 276762, 19, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 239, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (273.71, 104.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (224, 343, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959) [M::mem_pestat] mean and std.dev: (356.97, 167.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1204) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (92, 577, 1534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4418) [M::mem_pestat] mean and std.dev: (783.32, 744.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5860) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 231, 755) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1883) [M::mem_pestat] mean and std.dev: (382.25, 262.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2447) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682736 sequences (100000243 bp) in 1.765 CPU sec, 1.778 real sec... [M::mem_process_seqs] Processed 681170 reads in 179.047 CPU sec, 5.799 real sec sorting block 66, #reads = 1069718 mem = 536877788 peak = 1080606720 [M::mem_process_seqs] Processed 679882 reads in 120.949 CPU sec, 3.882 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 276783, 5, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 277, 620) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1422) [M::mem_pestat] mean and std.dev: (405.06, 327.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1823) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 326, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940) [M::mem_pestat] mean and std.dev: (343.09, 164.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1183) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 269, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (295.10, 150.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1104) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 67, #reads = 1069768 mem = 536878361 peak = 1081892864 [M::process] read 683522 sequences (100000153 bp) in 2.525 CPU sec, 2.694 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 278055, 13, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 257, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (338.54, 200.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 322, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932) [M::mem_pestat] mean and std.dev: (340.33, 163.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1173) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1090, 1090, 1445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (380, 2155) [M::mem_pestat] mean and std.dev: (1154.08, 219.31) [M::mem_pestat] low and high boundaries for proper pairs: (25, 2510) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (372, 604, 616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1104) [M::mem_pestat] mean and std.dev: (459.62, 185.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1348) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683384 sequences (100000137 bp) in 1.838 CPU sec, 1.850 real sec... [M::mem_process_seqs] Processed 682204 reads in 182.001 CPU sec, 5.882 real sec [M::mem_process_seqs] Processed 682736 reads in 123.986 CPU sec, 3.974 real sec sorting block 68, #reads = 1070421 mem = 536879877 peak = 1081892864 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 278128, 13, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (227, 264, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (308.41, 147.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 319, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931) [M::mem_pestat] mean and std.dev: (336.82, 162.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1173) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (796, 796, 796) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (796, 796) [M::mem_pestat] mean and std.dev: (796.00, 0.00) [M::mem_pestat] low and high boundaries for proper pairs: (796, 796) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 363, 606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1354) [M::mem_pestat] mean and std.dev: (392.83, 198.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1728) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682998 sequences (100000182 bp) in 2.682 CPU sec, 2.828 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 277927, 13, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 399, 639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1615) [M::mem_pestat] mean and std.dev: (439.64, 339.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2103) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 319, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923) [M::mem_pestat] mean and std.dev: (336.21, 161.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1162) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 237, 646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1844) [M::mem_pestat] mean and std.dev: (318.42, 313.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2443) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (248, 330, 623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1373) [M::mem_pestat] mean and std.dev: (394.64, 235.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1748) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683570 sequences (100000276 bp) in 1.932 CPU sec, 1.941 real sec... [M::mem_process_seqs] Processed 683522 reads in 183.431 CPU sec, 5.907 real sec [M::mem_process_seqs] Processed 683384 reads in 125.667 CPU sec, 4.032 real sec sorting block 69, #reads = 1070850 mem = 536880743 peak = 1081892864 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 278168, 20, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 291, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1313) [M::mem_pestat] mean and std.dev: (317.33, 204.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 318, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (335.89, 161.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1158) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (504, 1921, 1921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4755) [M::mem_pestat] mean and std.dev: (1362.75, 1014.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6172) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 297, 985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2495) [M::mem_pestat] mean and std.dev: (561.84, 567.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3250) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682994 sequences (100000247 bp) in 2.536 CPU sec, 2.768 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 278254, 7, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 324, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1229) [M::mem_pestat] mean and std.dev: (314.00, 175.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 319, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (337.59, 163.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1177) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 323, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (291.58, 106.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1004) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683124 sequences (100000006 bp) in 1.811 CPU sec, 1.835 real sec... [M::mem_process_seqs] Processed 682998 reads in 183.218 CPU sec, 5.898 real sec [M::mem_process_seqs] Processed 683570 reads in 125.960 CPU sec, 4.029 real sec sorting block 70, #reads = 1069698 mem = 536879160 peak = 1081892864 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 277733, 14, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (256, 551, 1547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4129) [M::mem_pestat] mean and std.dev: (750.35, 676.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 320, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (338.51, 163.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1175) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (487, 1584, 1821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4489) [M::mem_pestat] mean and std.dev: (1341.62, 1103.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5823) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 289, 602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1390) [M::mem_pestat] mean and std.dev: (389.48, 326.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1784) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680928 sequences (100000294 bp) in 2.594 CPU sec, 2.787 real sec... [M::process] read 681178 sequences (100000208 bp) in 1.667 CPU sec, 1.676 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 277508, 8, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (247, 545, 853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2065) [M::mem_pestat] mean and std.dev: (540.84, 472.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 321, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (339.75, 164.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1183) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 399, 669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1547) [M::mem_pestat] mean and std.dev: (501.33, 383.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2035) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682994 reads in 182.706 CPU sec, 5.854 real sec sorting block 71, #reads = 1071033 mem = 536879227 peak = 1081892864 [M::mem_process_seqs] Processed 683124 reads in 123.795 CPU sec, 3.953 real sec sorting block 72, #reads = 1071006 mem = 536885392 peak = 1120202752 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 277162, 11, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 397, 737) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1805) [M::mem_pestat] mean and std.dev: (408.71, 242.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2339) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (218, 337, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959) [M::mem_pestat] mean and std.dev: (352.00, 166.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1206) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (469, 469, 584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (239, 814) [M::mem_pestat] mean and std.dev: (523.67, 107.64) [M::mem_pestat] low and high boundaries for proper pairs: (93, 954) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 313, 542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1258) [M::mem_pestat] mean and std.dev: (357.96, 185.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1616) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682432 sequences (100000074 bp) in 2.530 CPU sec, 2.675 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 277210, 9, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 424, 603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1417) [M::mem_pestat] mean and std.dev: (434.25, 274.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1824) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 331, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947) [M::mem_pestat] mean and std.dev: (346.98, 164.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1191) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 275, 621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1443) [M::mem_pestat] mean and std.dev: (316.31, 183.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1854) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682582 sequences (100000048 bp) in 1.854 CPU sec, 1.863 real sec... [M::mem_process_seqs] Processed 680928 reads in 179.770 CPU sec, 5.735 real sec [M::mem_process_seqs] Processed 681178 reads in 123.236 CPU sec, 3.913 real sec sorting block 73, #reads = 1069230 mem = 536878846 peak = 1120202752 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 277903, 12, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 334, 558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1276) [M::mem_pestat] mean and std.dev: (327.18, 176.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 322, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (340.53, 164.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1177) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (110, 460, 1198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3374) [M::mem_pestat] mean and std.dev: (614.08, 686.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4462) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 219, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950) [M::mem_pestat] mean and std.dev: (289.23, 170.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1214) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681780 sequences (100000062 bp) in 2.563 CPU sec, 2.731 real sec... [M::process] read 681508 sequences (100000020 bp) in 1.715 CPU sec, 1.728 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 277750, 11, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 362, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1106) [M::mem_pestat] mean and std.dev: (340.76, 190.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 320, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (339.70, 164.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1176) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (226, 705, 784) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1900) [M::mem_pestat] mean and std.dev: (569.55, 336.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2458) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 451, 603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1405) [M::mem_pestat] mean and std.dev: (425.31, 251.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1806) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682432 reads in 183.243 CPU sec, 5.855 real sec [M::mem_process_seqs] Processed 682582 reads in 124.562 CPU sec, 4.010 real sec sorting block 74, #reads = 1070237 mem = 536887521 peak = 1120202752 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 277424, 4, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 226, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (258.09, 156.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1171) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 330, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952) [M::mem_pestat] mean and std.dev: (347.25, 165.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1198) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 265, 727) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1729) [M::mem_pestat] mean and std.dev: (417.04, 274.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2230) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682104 sequences (100000093 bp) in 2.473 CPU sec, 2.622 real sec... [M::process] read 682342 sequences (100000039 bp) in 1.915 CPU sec, 1.921 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 277606, 11, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 329, 681) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1689) [M::mem_pestat] mean and std.dev: (479.75, 358.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2193) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 329, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949) [M::mem_pestat] mean and std.dev: (346.75, 165.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1194) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (514, 590, 2980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7912) [M::mem_pestat] mean and std.dev: (1226.45, 1184.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10378) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 342, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1237) [M::mem_pestat] mean and std.dev: (363.43, 163.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1579) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681780 reads in 187.311 CPU sec, 6.022 real sec sorting block 75, #reads = 1069647 mem = 536881667 peak = 1120202752 [M::mem_process_seqs] Processed 681508 reads in 123.658 CPU sec, 3.928 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 277430, 12, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 381, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1153) [M::mem_pestat] mean and std.dev: (377.45, 243.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 323, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (341.11, 163.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (949, 1391, 7981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 22045) [M::mem_pestat] mean and std.dev: (3318.67, 3322.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 29077) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 358, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1072) [M::mem_pestat] mean and std.dev: (304.13, 133.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1368) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 76, #reads = 1069771 mem = 536880660 peak = 1120202752 [M::process] read 682574 sequences (100000098 bp) in 2.632 CPU sec, 2.766 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 277745, 17, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 411, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1143) [M::mem_pestat] mean and std.dev: (341.89, 154.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 682506 sequences (100000202 bp) in 1.919 CPU sec, 1.918 real sec... [M::mem_pestat] (25, 50, 75) percentile: (209, 321, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (340.75, 164.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1181) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (458, 798, 2584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6836) [M::mem_pestat] mean and std.dev: (1368.44, 1525.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8962) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (264, 344, 1487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3933) [M::mem_pestat] mean and std.dev: (769.75, 706.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5156) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682104 reads in 188.355 CPU sec, 6.008 real sec [M::mem_process_seqs] Processed 682342 reads in 128.108 CPU sec, 4.061 real sec sorting block 77, #reads = 1068959 mem = 536881865 peak = 1120202752 [M::process] read 682750 sequences (100000300 bp) in 2.479 CPU sec, 2.607 real sec... [M::process] read 682602 sequences (100000280 bp) in 1.901 CPU sec, 1.909 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 277740, 13, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 234, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (254.18, 105.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 318, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (337.84, 162.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1167) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (166, 351, 837) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2179) [M::mem_pestat] mean and std.dev: (357.15, 312.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2850) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 323, 597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1395) [M::mem_pestat] mean and std.dev: (382.15, 262.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1794) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682506 reads in 105.971 CPU sec, 3.350 real sec sorting block 78, #reads = 1068153 mem = 536885626 peak = 1120202752 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 277756, 12, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (253, 305, 1146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2932) [M::mem_pestat] mean and std.dev: (533.42, 492.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3825) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 316, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (335.83, 161.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1155) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (144, 1045, 1678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4746) [M::mem_pestat] mean and std.dev: (777.73, 686.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6280) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 239, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849) [M::mem_pestat] mean and std.dev: (263.41, 130.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1077) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 277936, 11, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 283, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973) [M::mem_pestat] mean and std.dev: (329.31, 197.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1240) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 316, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (335.16, 161.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1400, 1400, 2783) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5549) [M::mem_pestat] mean and std.dev: (1539.30, 688.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6932) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (266, 295, 570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1178) [M::mem_pestat] mean and std.dev: (350.41, 195.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1482) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682044 sequences (100000120 bp) in 2.017 CPU sec, 2.026 real sec... [M::mem_process_seqs] Processed 682574 reads in 291.778 CPU sec, 9.303 real sec [M::mem_process_seqs] Processed 682750 reads in 148.613 CPU sec, 4.757 real sec sorting block 79, #reads = 1068478 mem = 536881201 peak = 1120202752 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 278241, 19, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 293, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1194) [M::mem_pestat] mean and std.dev: (358.45, 245.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 317, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (336.89, 162.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1163) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (131, 1105, 1828) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5222) [M::mem_pestat] mean and std.dev: (1335.00, 1510.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7377) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (245, 357, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1100) [M::mem_pestat] mean and std.dev: (384.31, 198.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1385) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682406 sequences (100000034 bp) in 2.621 CPU sec, 2.778 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 277684, 14, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 248, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (235.92, 107.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 320, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (339.54, 163.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1169) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (629, 1220, 1596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3530) [M::mem_pestat] mean and std.dev: (1014.31, 526.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4497) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 358, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1171) [M::mem_pestat] mean and std.dev: (378.54, 242.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1500) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682180 sequences (100000022 bp) in 1.880 CPU sec, 1.893 real sec... [M::mem_process_seqs] Processed 682602 reads in 186.648 CPU sec, 5.959 real sec [M::mem_process_seqs] Processed 682044 reads in 125.630 CPU sec, 3.992 real sec sorting block 80, #reads = 1069015 mem = 536881284 peak = 1120202752 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 277663, 11, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 315, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066) [M::mem_pestat] mean and std.dev: (309.18, 176.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1365) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 320, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (340.11, 164.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1176) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (911, 2574, 2615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6023) [M::mem_pestat] mean and std.dev: (2007.27, 1132.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7727) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (264, 352, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (329.47, 123.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682790 sequences (100000274 bp) in 2.767 CPU sec, 2.917 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 277915, 13, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 269, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (251.72, 85.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 687376 sequences (100000077 bp) in 1.914 CPU sec, 1.919 real sec... [M::mem_pestat] (25, 50, 75) percentile: (208, 320, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937) [M::mem_pestat] mean and std.dev: (340.51, 165.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1180) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (305, 305, 1797) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4781) [M::mem_pestat] mean and std.dev: (1056.85, 1102.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6273) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (258, 552, 759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1761) [M::mem_pestat] mean and std.dev: (532.86, 340.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2262) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682406 reads in 182.918 CPU sec, 5.811 real sec [M::mem_process_seqs] Processed 682180 reads in 124.080 CPU sec, 3.931 real sec sorting block 81, #reads = 1067879 mem = 536883020 peak = 1120202752 [M::process] read 684910 sequences (100000099 bp) in 2.548 CPU sec, 2.676 real sec... [M::process] read 684768 sequences (100000071 bp) in 1.970 CPU sec, 1.961 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 280643, 13, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 262, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (266.08, 125.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 292, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (319.94, 163.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (757, 2045, 2792) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6862) [M::mem_pestat] mean and std.dev: (1767.69, 1072.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8897) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (264, 414, 613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1311) [M::mem_pestat] mean and std.dev: (503.56, 342.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1875) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687376 reads in 113.307 CPU sec, 3.578 real sec sorting block 82, #reads = 1068419 mem = 536881368 peak = 1120202752 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 278395, 22, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 311, 657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1509) [M::mem_pestat] mean and std.dev: (388.15, 280.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1935) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 314, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (336.82, 165.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1169) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (729, 847, 2570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6252) [M::mem_pestat] mean and std.dev: (1394.67, 1485.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8093) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (248, 343, 673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1523) [M::mem_pestat] mean and std.dev: (408.60, 252.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1948) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 279796, 22, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 240, 707) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1813) [M::mem_pestat] mean and std.dev: (329.33, 293.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2366) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 313, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970) [M::mem_pestat] mean and std.dev: (338.42, 171.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1227) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (729, 1366, 5557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15213) [M::mem_pestat] mean and std.dev: (3002.55, 2734.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20041) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 325, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1059) [M::mem_pestat] mean and std.dev: (332.78, 213.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1340) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685654 sequences (100000244 bp) in 2.031 CPU sec, 2.037 real sec... [M::mem_process_seqs] Processed 682790 reads in 300.099 CPU sec, 9.555 real sec [M::mem_process_seqs] Processed 684910 reads in 155.920 CPU sec, 4.988 real sec sorting block 83, #reads = 1066721 mem = 536879239 peak = 1120202752 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 279955, 13, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (272, 470, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950) [M::mem_pestat] mean and std.dev: (368.56, 146.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1176) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 313, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968) [M::mem_pestat] mean and std.dev: (338.54, 170.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1224) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (383, 1053, 1308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3158) [M::mem_pestat] mean and std.dev: (756.17, 496.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4083) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 284, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967) [M::mem_pestat] mean and std.dev: (298.00, 151.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1217) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686306 sequences (100000091 bp) in 2.699 CPU sec, 2.866 real sec... [M::process] read 686108 sequences (100000193 bp) in 1.869 CPU sec, 1.870 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 279994, 11, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 339, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (323.42, 167.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 306, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943) [M::mem_pestat] mean and std.dev: (331.84, 167.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1192) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (215, 315, 1339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3587) [M::mem_pestat] mean and std.dev: (486.56, 405.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4711) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 339, 558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1250) [M::mem_pestat] mean and std.dev: (352.51, 204.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1596) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684768 reads in 192.950 CPU sec, 6.143 real sec sorting block 84, #reads = 1066311 mem = 536878782 peak = 1120202752 [M::mem_process_seqs] Processed 685654 reads in 129.130 CPU sec, 4.110 real sec sorting block 85, #reads = 1067223 mem = 536878004 peak = 1120202752 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 280288, 8, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 266, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (278.43, 153.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1064) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 301, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (327.20, 165.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1173) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 339, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037) [M::mem_pestat] mean and std.dev: (362.35, 173.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1313) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686144 sequences (100000166 bp) in 2.816 CPU sec, 2.985 real sec... [M::process] read 683436 sequences (100000199 bp) in 1.760 CPU sec, 1.779 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 279712, 26, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (277, 371, 630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1336) [M::mem_pestat] mean and std.dev: (443.60, 253.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 303, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932) [M::mem_pestat] mean and std.dev: (329.02, 166.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1178) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (69, 534, 1053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3021) [M::mem_pestat] mean and std.dev: (528.64, 554.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4005) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 339, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1067) [M::mem_pestat] mean and std.dev: (368.31, 239.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1359) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686306 reads in 188.712 CPU sec, 6.011 real sec [M::mem_process_seqs] Processed 686108 reads in 133.095 CPU sec, 4.244 real sec sorting block 86, #reads = 1068386 mem = 536884183 peak = 1120202752 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 280469, 17, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 371, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1361) [M::mem_pestat] mean and std.dev: (397.68, 330.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 301, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (325.73, 163.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (274, 504, 838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1966) [M::mem_pestat] mean and std.dev: (434.14, 225.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2530) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 435, 713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1745) [M::mem_pestat] mean and std.dev: (442.04, 279.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2261) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683714 sequences (100000111 bp) in 2.391 CPU sec, 2.552 real sec... [M::process] read 686102 sequences (100000115 bp) in 1.639 CPU sec, 1.643 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 279404, 10, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 232, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959) [M::mem_pestat] mean and std.dev: (276.07, 218.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1232) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 321, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969) [M::mem_pestat] mean and std.dev: (342.71, 169.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1224) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (128, 446, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1082) [M::mem_pestat] mean and std.dev: (338.44, 208.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1400) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 272, 548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1208) [M::mem_pestat] mean and std.dev: (361.85, 245.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1538) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686144 reads in 189.024 CPU sec, 6.049 real sec [M::mem_process_seqs] Processed 683436 reads in 127.855 CPU sec, 4.091 real sec sorting block 87, #reads = 1068102 mem = 536880324 peak = 1120202752 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 279224, 12, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (374, 496, 591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025) [M::mem_pestat] mean and std.dev: (459.43, 179.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1242) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 319, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954) [M::mem_pestat] mean and std.dev: (340.01, 166.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1204) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (106, 515, 1973) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5707) [M::mem_pestat] mean and std.dev: (1098.08, 1260.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7574) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (276, 691, 1277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3279) [M::mem_pestat] mean and std.dev: (774.70, 616.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4280) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686518 sequences (100000122 bp) in 2.334 CPU sec, 2.465 real sec... [M::process] read 685482 sequences (100000153 bp) in 1.620 CPU sec, 1.626 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 280304, 15, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 307, 811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2043) [M::mem_pestat] mean and std.dev: (456.18, 398.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 301, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (326.36, 163.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1162) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (194, 816, 5167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15113) [M::mem_pestat] mean and std.dev: (1834.00, 2094.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20086) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 350, 605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1411) [M::mem_pestat] mean and std.dev: (345.81, 224.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1814) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683714 reads in 187.346 CPU sec, 5.968 real sec sorting block 88, #reads = 1067066 mem = 536878305 peak = 1120202752 [M::mem_process_seqs] Processed 686102 reads in 125.917 CPU sec, 4.008 real sec sorting block 89, #reads = 1068193 mem = 536881005 peak = 1120202752 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 280734, 17, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (297, 457, 844) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1938) [M::mem_pestat] mean and std.dev: (560.71, 430.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 298, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (323.50, 161.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (528, 847, 1068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2148) [M::mem_pestat] mean and std.dev: (901.76, 530.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3024) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 393, 799) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1951) [M::mem_pestat] mean and std.dev: (521.95, 449.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2527) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685084 sequences (100000082 bp) in 2.794 CPU sec, 2.909 real sec... [M::process] read 683370 sequences (100000268 bp) in 1.917 CPU sec, 1.926 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 280376, 7, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 250, 1136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3028) [M::mem_pestat] mean and std.dev: (606.16, 572.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3974) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 305, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (329.35, 164.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1180) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 287, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1244) [M::mem_pestat] mean and std.dev: (331.45, 226.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1608) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686518 reads in 189.961 CPU sec, 6.020 real sec [M::mem_process_seqs] Processed 685482 reads in 124.694 CPU sec, 3.956 real sec sorting block 90, #reads = 1067159 mem = 536878291 peak = 1120202752 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 279599, 17, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 253, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (280.12, 120.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 309, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (332.19, 165.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1186) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 86, 690) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2006) [M::mem_pestat] mean and std.dev: (353.19, 499.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2664) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 352, 621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1461) [M::mem_pestat] mean and std.dev: (397.95, 230.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1881) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682220 sequences (100000290 bp) in 2.510 CPU sec, 2.608 real sec... [M::process] read 684104 sequences (100000125 bp) in 1.870 CPU sec, 1.871 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 278653, 5, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 303, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1072) [M::mem_pestat] mean and std.dev: (323.37, 196.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1361) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 318, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954) [M::mem_pestat] mean and std.dev: (339.83, 166.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1204) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 237, 642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1570) [M::mem_pestat] mean and std.dev: (450.70, 407.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2082) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685084 reads in 187.569 CPU sec, 5.951 real sec [M::mem_process_seqs] Processed 683370 reads in 123.940 CPU sec, 3.944 real sec sorting block 91, #reads = 1066019 mem = 536879524 peak = 1120202752 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278103, 16, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (264, 367, 576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1200) [M::mem_pestat] mean and std.dev: (403.16, 242.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 329, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973) [M::mem_pestat] mean and std.dev: (348.52, 169.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1227) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (403, 730, 905) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1909) [M::mem_pestat] mean and std.dev: (591.64, 294.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2411) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 346, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (377.06, 169.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683810 sequences (100000018 bp) in 2.593 CPU sec, 2.678 real sec... [M::process] read 682192 sequences (100000030 bp) in 1.920 CPU sec, 1.918 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 279041, 12, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 342, 620) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1436) [M::mem_pestat] mean and std.dev: (349.87, 200.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1844) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 313, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945) [M::mem_pestat] mean and std.dev: (336.11, 166.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1193) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (82, 258, 4388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13000) [M::mem_pestat] mean and std.dev: (1512.17, 2117.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17306) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 363, 784) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1882) [M::mem_pestat] mean and std.dev: (421.05, 257.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2431) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682220 reads in 187.493 CPU sec, 5.946 real sec [M::mem_process_seqs] Processed 684104 reads in 125.225 CPU sec, 3.978 real sec sorting block 92, #reads = 1065445 mem = 536879748 peak = 1120202752 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 279018, 27, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 386, 1954) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5518) [M::mem_pestat] mean and std.dev: (1073.67, 1235.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7300) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 315, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (337.19, 166.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1191) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (257, 543, 2516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7034) [M::mem_pestat] mean and std.dev: (1294.89, 1503.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9293) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 328, 618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1424) [M::mem_pestat] mean and std.dev: (368.68, 218.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1827) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683024 sequences (100000155 bp) in 2.444 CPU sec, 2.533 real sec... [M::process] read 682630 sequences (100000071 bp) in 1.850 CPU sec, 1.854 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 278399, 14, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 304, 729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1803) [M::mem_pestat] mean and std.dev: (464.86, 390.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2340) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 324, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951) [M::mem_pestat] mean and std.dev: (343.41, 165.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1198) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (233, 655, 1128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2918) [M::mem_pestat] mean and std.dev: (747.69, 640.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3813) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 218, 800) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2018) [M::mem_pestat] mean and std.dev: (485.97, 466.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2627) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683810 reads in 187.445 CPU sec, 5.955 real sec sorting block 93, #reads = 1065144 mem = 536876948 peak = 1120202752 [M::mem_process_seqs] Processed 682192 reads in 124.892 CPU sec, 3.967 real sec sorting block 94, #reads = 1064936 mem = 536881644 peak = 1120202752 [M::process] read 682490 sequences (100000019 bp) in 2.329 CPU sec, 2.434 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 277512, 11, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 330, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (306.03, 119.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1097) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 315, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (335.52, 161.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1169) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (2036, 2036, 2036) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (2036, 2036) [M::mem_pestat] mean and std.dev: (2036.00, 0.00) [M::mem_pestat] low and high boundaries for proper pairs: (2036, 2036) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 302, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (276.26, 80.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681456 sequences (100000254 bp) in 1.580 CPU sec, 1.573 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 278162, 15, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 295, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974) [M::mem_pestat] mean and std.dev: (279.30, 129.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1226) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 321, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949) [M::mem_pestat] mean and std.dev: (341.76, 165.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1196) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (623, 725, 1282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2600) [M::mem_pestat] mean and std.dev: (885.29, 531.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3259) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 329, 596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1382) [M::mem_pestat] mean and std.dev: (400.95, 281.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1775) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 278489, 12, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 288, 512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1130) [M::mem_pestat] mean and std.dev: (311.00, 144.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 318, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (338.53, 163.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1182) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (608, 1220, 6000) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16784) [M::mem_pestat] mean and std.dev: (2626.67, 2690.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22176) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 292, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1239) [M::mem_pestat] mean and std.dev: (332.94, 220.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1587) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683024 reads in 272.983 CPU sec, 8.723 real sec [M::mem_process_seqs] Processed 682490 reads in 209.530 CPU sec, 6.713 real sec [M::mem_process_seqs] Processed 682630 reads in 232.940 CPU sec, 7.471 real sec sorting block 95, #reads = 1064537 mem = 536878271 peak = 1120202752 [M::process] read 683456 sequences (100000150 bp) in 2.731 CPU sec, 2.915 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 277901, 15, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 198, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (200.95, 89.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 331, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975) [M::mem_pestat] mean and std.dev: (349.78, 167.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1229) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1038, 2597, 3929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9711) [M::mem_pestat] mean and std.dev: (2412.07, 1735.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12602) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (277, 290, 974) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2368) [M::mem_pestat] mean and std.dev: (571.87, 456.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3065) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 96, #reads = 1063752 mem = 536884015 peak = 1120202752 [M::process] read 683154 sequences (100000110 bp) in 1.926 CPU sec, 1.990 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 278396, 17, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 268, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (251.40, 103.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 312, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931) [M::mem_pestat] mean and std.dev: (334.23, 163.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (580, 1745, 9112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 26176) [M::mem_pestat] mean and std.dev: (4329.47, 4117.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 34708) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 389, 628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1524) [M::mem_pestat] mean and std.dev: (393.21, 284.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1972) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682088 sequences (100000120 bp) in 1.531 CPU sec, 1.537 real sec... [M::mem_process_seqs] Processed 681456 reads in 199.240 CPU sec, 6.356 real sec [M::mem_process_seqs] Processed 683456 reads in 135.126 CPU sec, 4.278 real sec sorting block 97, #reads = 1063466 mem = 536878348 peak = 1120202752 [M::process] read 682694 sequences (100000088 bp) in 2.240 CPU sec, 2.384 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 278269, 17, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 327, 776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1868) [M::mem_pestat] mean and std.dev: (405.16, 278.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2414) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 314, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (334.99, 162.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1168) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (448, 1335, 5723) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16273) [M::mem_pestat] mean and std.dev: (2807.59, 2834.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21548) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 342, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965) [M::mem_pestat] mean and std.dev: (301.92, 168.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1223) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683006 sequences (100000007 bp) in 1.578 CPU sec, 1.578 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 278476, 10, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 298, 577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1317) [M::mem_pestat] mean and std.dev: (380.49, 257.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 319, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947) [M::mem_pestat] mean and std.dev: (340.51, 166.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1194) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (215, 1316, 2768) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7874) [M::mem_pestat] mean and std.dev: (1339.00, 1220.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10427) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 327, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (294.04, 107.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683154 reads in 186.380 CPU sec, 5.940 real sec sorting block 98, #reads = 1064155 mem = 536880089 peak = 1120202752 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 277663, 20, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 258, 868) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2178) [M::mem_pestat] mean and std.dev: (403.06, 300.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2833) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 323, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956) [M::mem_pestat] mean and std.dev: (343.90, 166.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1205) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (174, 711, 2850) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8202) [M::mem_pestat] mean and std.dev: (1338.30, 1525.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10878) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 474, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1181) [M::mem_pestat] mean and std.dev: (380.73, 183.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1507) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682826 sequences (100000295 bp) in 1.824 CPU sec, 1.826 real sec... [M::mem_process_seqs] Processed 682694 reads in 202.826 CPU sec, 6.449 real sec [M::mem_process_seqs] Processed 682088 reads in 224.825 CPU sec, 7.214 real sec sorting block 99, #reads = 1064360 mem = 536882825 peak = 1120202752 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 278443, 13, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 303, 795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1935) [M::mem_pestat] mean and std.dev: (499.20, 408.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 681640 sequences (100000277 bp) in 2.275 CPU sec, 2.370 real sec... [M::mem_pestat] (25, 50, 75) percentile: (205, 316, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943) [M::mem_pestat] mean and std.dev: (338.97, 166.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1189) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (435, 737, 2568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6834) [M::mem_pestat] mean and std.dev: (1368.62, 970.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8967) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 390, 880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2202) [M::mem_pestat] mean and std.dev: (507.65, 399.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2863) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689760 sequences (100000043 bp) in 1.650 CPU sec, 1.647 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 278641, 14, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 421, 698) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1564) [M::mem_pestat] mean and std.dev: (461.62, 306.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1997) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 317, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947) [M::mem_pestat] mean and std.dev: (340.49, 167.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1194) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (175, 997, 1674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4672) [M::mem_pestat] mean and std.dev: (1010.14, 787.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6171) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 369, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1096) [M::mem_pestat] mean and std.dev: (368.73, 194.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1386) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683006 reads in 183.498 CPU sec, 5.886 real sec [M::mem_process_seqs] Processed 682826 reads in 122.002 CPU sec, 3.887 real sec sorting block 100, #reads = 1064868 mem = 536885747 peak = 1120202752 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 277688, 19, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 242, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1153) [M::mem_pestat] mean and std.dev: (314.60, 186.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 327, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970) [M::mem_pestat] mean and std.dev: (348.96, 171.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1223) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (648, 950, 2506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6222) [M::mem_pestat] mean and std.dev: (1616.63, 1606.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8080) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 305, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1117) [M::mem_pestat] mean and std.dev: (325.79, 209.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1423) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 690160 sequences (100000294 bp) in 2.707 CPU sec, 2.826 real sec... [M::process] read 687966 sequences (100000141 bp) in 1.950 CPU sec, 1.954 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281731, 5, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 326, 1016) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2660) [M::mem_pestat] mean and std.dev: (469.12, 407.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 283, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (309.13, 156.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (295, 375, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (350.13, 130.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681640 reads in 197.900 CPU sec, 6.269 real sec sorting block 101, #reads = 1064459 mem = 536878006 peak = 1120202752 [M::mem_process_seqs] Processed 689760 reads in 139.877 CPU sec, 4.454 real sec [M::process] read 688326 sequences (100000082 bp) in 2.265 CPU sec, 2.371 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 281778, 10, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 353, 633) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1467) [M::mem_pestat] mean and std.dev: (360.27, 225.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1884) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 283, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (308.60, 155.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (826, 856, 887) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (704, 1009) [M::mem_pestat] mean and std.dev: (851.17, 20.90) [M::mem_pestat] low and high boundaries for proper pairs: (643, 1070) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 258, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062) [M::mem_pestat] mean and std.dev: (292.32, 218.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1366) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687224 sequences (100000292 bp) in 1.590 CPU sec, 1.592 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 280996, 9, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 403, 623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1567) [M::mem_pestat] mean and std.dev: (356.35, 241.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2039) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 293, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (316.10, 156.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 363, 2315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6539) [M::mem_pestat] mean and std.dev: (1096.58, 1275.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8651) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 281703, 6, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 264, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921) [M::mem_pestat] mean and std.dev: (292.15, 201.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1178) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 294, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (316.48, 157.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 332, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1109) [M::mem_pestat] mean and std.dev: (341.04, 188.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1411) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690160 reads in 300.732 CPU sec, 9.614 real sec sorting block 102, #reads = 1061075 mem = 536880317 peak = 1120202752 [M::mem_process_seqs] Processed 687966 reads in 229.717 CPU sec, 7.387 real sec [M::mem_process_seqs] Processed 688326 reads in 255.099 CPU sec, 8.218 real sec sorting block 103, #reads = 1059104 mem = 536880452 peak = 1145507840 [M::process] read 687356 sequences (100000192 bp) in 2.309 CPU sec, 2.455 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 280957, 12, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 228, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (221.61, 67.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 299, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (320.57, 158.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1136) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (462, 503, 1526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3654) [M::mem_pestat] mean and std.dev: (662.09, 455.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4718) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 289, 1265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3395) [M::mem_pestat] mean and std.dev: (545.00, 555.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4460) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685660 sequences (100000117 bp) in 2.321 CPU sec, 2.406 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 280643, 10, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 330, 1245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3419) [M::mem_pestat] mean and std.dev: (665.09, 687.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 295, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (317.56, 157.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (407, 602, 1121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2549) [M::mem_pestat] mean and std.dev: (586.11, 358.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3263) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 286, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (276.63, 128.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685240 sequences (100000225 bp) in 1.830 CPU sec, 1.829 real sec... [M::mem_process_seqs] Processed 687224 reads in 203.105 CPU sec, 6.452 real sec sorting block 104, #reads = 1060619 mem = 536889297 peak = 1145507840 [M::mem_process_seqs] Processed 687356 reads in 137.457 CPU sec, 4.354 real sec sorting block 105, #reads = 1062548 mem = 536878692 peak = 1145507840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 280228, 9, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 242, 610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1504) [M::mem_pestat] mean and std.dev: (310.58, 222.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1951) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 305, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (326.70, 160.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 413, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1090) [M::mem_pestat] mean and std.dev: (367.63, 181.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1380) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686372 sequences (100000231 bp) in 2.460 CPU sec, 2.582 real sec... [M::process] read 686736 sequences (100000165 bp) in 1.490 CPU sec, 1.485 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 280174, 12, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 421, 780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1892) [M::mem_pestat] mean and std.dev: (503.14, 351.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 308, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (328.47, 161.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 1410, 1592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4720) [M::mem_pestat] mean and std.dev: (868.42, 746.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6284) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 238, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (25, 490) [M::mem_pestat] mean and std.dev: (223.80, 78.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685660 reads in 192.426 CPU sec, 6.132 real sec [M::mem_process_seqs] Processed 685240 reads in 124.391 CPU sec, 3.966 real sec sorting block 106, #reads = 1063165 mem = 536882769 peak = 1145507840 [M::process] read 686520 sequences (100000171 bp) in 2.143 CPU sec, 2.235 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 280458, 7, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 592, 861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2135) [M::mem_pestat] mean and std.dev: (580.16, 459.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2772) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 300, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (321.85, 158.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 323, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (317.36, 137.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686372 reads in 107.328 CPU sec, 3.474 real sec sorting block 107, #reads = 1064538 mem = 536878726 peak = 1145507840 [M::process] read 685648 sequences (100000166 bp) in 2.286 CPU sec, 2.396 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 280496, 14, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 235, 717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1855) [M::mem_pestat] mean and std.dev: (426.20, 471.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 298, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (321.69, 159.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (206, 530, 1086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2846) [M::mem_pestat] mean and std.dev: (616.57, 495.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3726) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (329, 621, 2053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5501) [M::mem_pestat] mean and std.dev: (1005.69, 1060.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7225) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683308 sequences (100000025 bp) in 1.733 CPU sec, 1.742 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 280221, 15, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 278, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1167) [M::mem_pestat] mean and std.dev: (316.30, 194.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 300, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (322.40, 159.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (217, 258, 647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1507) [M::mem_pestat] mean and std.dev: (271.50, 151.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1937) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 234, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961) [M::mem_pestat] mean and std.dev: (305.27, 244.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1282) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686736 reads in 212.480 CPU sec, 6.800 real sec [M::mem_process_seqs] Processed 686520 reads in 230.654 CPU sec, 7.420 real sec sorting block 108, #reads = 1065372 mem = 536879260 peak = 1145507840 [M::process] read 683562 sequences (100000227 bp) in 2.498 CPU sec, 2.632 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 280174, 18, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 342, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005) [M::mem_pestat] mean and std.dev: (333.82, 220.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1288) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 305, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (327.46, 162.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (3, 542, 1005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3009) [M::mem_pestat] mean and std.dev: (654.83, 776.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4011) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 291, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (323.36, 168.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685648 reads in 114.362 CPU sec, 3.701 real sec sorting block 109, #reads = 1064678 mem = 536883491 peak = 1145507840 [M::process] read 684570 sequences (100000190 bp) in 2.141 CPU sec, 2.358 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 279346, 6, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 184, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (224.03, 89.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 321, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952) [M::mem_pestat] mean and std.dev: (340.90, 166.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1201) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 302, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (284.59, 124.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1019) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683240 sequences (100000012 bp) in 1.582 CPU sec, 1.590 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 279359, 12, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 330, 682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1724) [M::mem_pestat] mean and std.dev: (344.39, 234.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2245) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 322, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967) [M::mem_pestat] mean and std.dev: (343.02, 168.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1221) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (690, 1144, 3234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8322) [M::mem_pestat] mean and std.dev: (1792.08, 1426.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10866) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 260, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (233.58, 76.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683308 reads in 209.463 CPU sec, 6.697 real sec [M::mem_process_seqs] Processed 683562 reads in 232.241 CPU sec, 7.484 real sec sorting block 110, #reads = 1064523 mem = 536879287 peak = 1145507840 [M::process] read 683488 sequences (100000030 bp) in 2.353 CPU sec, 2.477 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 279437, 20, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 360, 963) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2449) [M::mem_pestat] mean and std.dev: (543.38, 531.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3192) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 312, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (333.68, 164.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1177) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (3, 192, 5084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15246) [M::mem_pestat] mean and std.dev: (2025.25, 3224.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20327) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (253, 409, 574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1216) [M::mem_pestat] mean and std.dev: (440.39, 228.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1537) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684570 reads in 105.408 CPU sec, 3.442 real sec sorting block 111, #reads = 1064564 mem = 536880616 peak = 1145507840 [M::process] read 683214 sequences (100000132 bp) in 2.333 CPU sec, 2.438 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 278665, 13, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (240, 304, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (330.74, 132.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 315, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (337.46, 166.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1191) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (802, 1476, 1641) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3319) [M::mem_pestat] mean and std.dev: (1209.67, 530.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4158) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 342, 954) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2400) [M::mem_pestat] mean and std.dev: (527.06, 469.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3123) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683178 sequences (100000037 bp) in 1.510 CPU sec, 1.511 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 279357, 23, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 319, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1083) [M::mem_pestat] mean and std.dev: (305.23, 128.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1377) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 319, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955) [M::mem_pestat] mean and std.dev: (341.05, 168.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1205) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (550, 696, 1212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2536) [M::mem_pestat] mean and std.dev: (661.15, 424.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3198) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 282, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (288.14, 117.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683488 reads in 205.032 CPU sec, 6.553 real sec [M::mem_process_seqs] Processed 683240 reads in 223.546 CPU sec, 7.264 real sec sorting block 112, #reads = 1064078 mem = 536878882 peak = 1145507840 [M::process] read 681476 sequences (100000079 bp) in 2.329 CPU sec, 2.437 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 278749, 19, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 330, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1149) [M::mem_pestat] mean and std.dev: (300.24, 189.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 318, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955) [M::mem_pestat] mean and std.dev: (339.98, 167.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1205) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (735, 808, 1747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3771) [M::mem_pestat] mean and std.dev: (1194.83, 938.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4950) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (123, 259, 1043) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2883) [M::mem_pestat] mean and std.dev: (403.16, 477.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3803) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682370 sequences (100000003 bp) in 1.806 CPU sec, 1.814 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 277512, 16, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 309, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1131) [M::mem_pestat] mean and std.dev: (335.62, 225.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1443) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 333, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981) [M::mem_pestat] mean and std.dev: (352.07, 171.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1237) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (447, 1066, 1555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3771) [M::mem_pestat] mean and std.dev: (992.31, 743.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4879) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 267, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1109) [M::mem_pestat] mean and std.dev: (349.84, 235.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1408) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278668, 14, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (338, 538, 1164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2816) [M::mem_pestat] mean and std.dev: (645.06, 562.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 317, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (339.82, 167.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1193) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (195, 495, 725) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1785) [M::mem_pestat] mean and std.dev: (465.46, 278.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2315) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (306, 414, 745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1623) [M::mem_pestat] mean and std.dev: (450.90, 295.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2062) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683214 reads in 261.906 CPU sec, 8.467 real sec [M::mem_process_seqs] Processed 681476 reads in 195.355 CPU sec, 6.257 real sec sorting block 113, #reads = 1064310 mem = 536883278 peak = 1145507840 [M::mem_process_seqs] Processed 683178 reads in 216.882 CPU sec, 6.972 real sec sorting block 114, #reads = 1063634 mem = 536879528 peak = 1145507840 [M::process] read 683534 sequences (100000193 bp) in 2.731 CPU sec, 2.927 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 278148, 19, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 304, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966) [M::mem_pestat] mean and std.dev: (336.34, 199.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1222) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 324, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965) [M::mem_pestat] mean and std.dev: (345.74, 169.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1217) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (711, 1215, 1977) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4509) [M::mem_pestat] mean and std.dev: (1205.68, 710.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5775) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 365, 696) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1702) [M::mem_pestat] mean and std.dev: (379.65, 278.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2205) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682370 reads in 108.106 CPU sec, 3.529 real sec [M::process] read 683162 sequences (100000092 bp) in 2.449 CPU sec, 2.607 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 279038, 13, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 472, 795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2033) [M::mem_pestat] mean and std.dev: (522.42, 393.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 314, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (337.54, 167.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1191) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (504, 1216, 2141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5415) [M::mem_pestat] mean and std.dev: (1176.23, 823.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7052) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 344, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988) [M::mem_pestat] mean and std.dev: (349.94, 156.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1242) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682942 sequences (100000184 bp) in 1.840 CPU sec, 1.834 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 278708, 8, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 307, 642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1536) [M::mem_pestat] mean and std.dev: (398.15, 259.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1983) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 317, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943) [M::mem_pestat] mean and std.dev: (339.33, 167.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1189) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 328, 607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1351) [M::mem_pestat] mean and std.dev: (355.36, 174.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1723) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683232 sequences (100000197 bp) in 1.770 CPU sec, 1.770 real sec... [M::mem_process_seqs] Processed 683534 reads in 210.697 CPU sec, 6.678 real sec sorting block 115, #reads = 1064233 mem = 536878849 peak = 1145507840 [M::mem_process_seqs] Processed 683162 reads in 122.662 CPU sec, 3.869 real sec sorting block 116, #reads = 1064967 mem = 536879896 peak = 1145507840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 277871, 14, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 309, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985) [M::mem_pestat] mean and std.dev: (366.53, 220.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1249) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 320, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965) [M::mem_pestat] mean and std.dev: (343.81, 171.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1218) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (183, 444, 1112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2970) [M::mem_pestat] mean and std.dev: (673.14, 632.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3899) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 323, 560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1290) [M::mem_pestat] mean and std.dev: (389.02, 229.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1655) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683132 sequences (100000263 bp) in 2.507 CPU sec, 2.619 real sec... [M::process] read 683378 sequences (100000047 bp) in 1.920 CPU sec, 1.924 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 279349, 10, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 242, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (257.70, 156.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 316, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966) [M::mem_pestat] mean and std.dev: (342.15, 172.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1220) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1094, 4714, 5256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13580) [M::mem_pestat] mean and std.dev: (3181.50, 1898.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17742) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (269, 503, 712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1598) [M::mem_pestat] mean and std.dev: (399.97, 189.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2041) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682942 reads in 188.060 CPU sec, 6.078 real sec [M::mem_process_seqs] Processed 683232 reads in 124.063 CPU sec, 4.029 real sec sorting block 117, #reads = 1064004 mem = 536877765 peak = 1145507840 [M::process] read 687122 sequences (100000262 bp) in 1.952 CPU sec, 2.057 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 278530, 17, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (262, 416, 658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1450) [M::mem_pestat] mean and std.dev: (489.15, 362.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1939) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 317, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963) [M::mem_pestat] mean and std.dev: (342.50, 171.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1216) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (125, 347, 1161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3233) [M::mem_pestat] mean and std.dev: (578.65, 575.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4269) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (272, 423, 723) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1625) [M::mem_pestat] mean and std.dev: (496.10, 316.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2076) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686536 sequences (100000244 bp) in 1.400 CPU sec, 1.401 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 278458, 9, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (274, 374, 550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1102) [M::mem_pestat] mean and std.dev: (354.31, 152.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1378) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 315, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959) [M::mem_pestat] mean and std.dev: (340.82, 171.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1211) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (263, 360, 875) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2099) [M::mem_pestat] mean and std.dev: (399.68, 240.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2711) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 280359, 13, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (255, 382, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (347.74, 151.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 295, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (320.66, 160.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1143) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (703, 946, 1764) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3886) [M::mem_pestat] mean and std.dev: (928.00, 586.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4947) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 314, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1113) [M::mem_pestat] mean and std.dev: (300.98, 144.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1416) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683132 reads in 277.697 CPU sec, 8.974 real sec sorting block 118, #reads = 1064243 mem = 536880209 peak = 1145507840 [M::mem_process_seqs] Processed 683378 reads in 221.426 CPU sec, 7.121 real sec [M::mem_process_seqs] Processed 687122 reads in 246.709 CPU sec, 7.940 real sec sorting block 119, #reads = 1056609 mem = 536879620 peak = 1145507840 [M::process] read 686172 sequences (100000170 bp) in 2.352 CPU sec, 2.505 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 280189, 13, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 324, 577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1297) [M::mem_pestat] mean and std.dev: (319.86, 243.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 302, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (324.18, 160.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (97, 306, 1149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3253) [M::mem_pestat] mean and std.dev: (512.33, 553.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4305) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 399, 1501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4169) [M::mem_pestat] mean and std.dev: (678.05, 566.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5503) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685494 sequences (100000027 bp) in 1.951 CPU sec, 2.037 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 280279, 20, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (234, 407, 1084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2784) [M::mem_pestat] mean and std.dev: (583.34, 539.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 304, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (326.25, 161.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1159) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (144, 1095, 2549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7359) [M::mem_pestat] mean and std.dev: (1340.21, 1578.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9764) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 305, 806) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1934) [M::mem_pestat] mean and std.dev: (396.44, 362.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2498) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685056 sequences (100000109 bp) in 1.400 CPU sec, 1.403 real sec... [M::mem_process_seqs] Processed 686536 reads in 188.518 CPU sec, 6.005 real sec [M::mem_process_seqs] Processed 686172 reads in 122.617 CPU sec, 3.893 real sec sorting block 120, #reads = 1063283 mem = 536880388 peak = 1145507840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 280025, 10, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 350, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1203) [M::mem_pestat] mean and std.dev: (370.52, 249.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 307, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (328.31, 161.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (410, 572, 1150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2630) [M::mem_pestat] mean and std.dev: (511.50, 332.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3370) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 216, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (245.69, 118.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684872 sequences (100000285 bp) in 2.533 CPU sec, 2.671 real sec... [M::process] read 683018 sequences (100000141 bp) in 2.008 CPU sec, 2.005 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 279924, 8, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 245, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047) [M::mem_pestat] mean and std.dev: (268.63, 169.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1343) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 310, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (330.38, 161.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1167) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 316, 1228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3306) [M::mem_pestat] mean and std.dev: (579.92, 669.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4345) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685494 reads in 182.479 CPU sec, 5.787 real sec sorting block 121, #reads = 1068390 mem = 536878942 peak = 1145507840 [M::mem_process_seqs] Processed 685056 reads in 122.827 CPU sec, 3.934 real sec sorting block 122, #reads = 1069795 mem = 536883919 peak = 1145507840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 279360, 12, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 309, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (277.31, 101.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1075) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 312, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940) [M::mem_pestat] mean and std.dev: (333.41, 164.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1187) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (305, 721, 721) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1553) [M::mem_pestat] mean and std.dev: (474.45, 294.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1969) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 233, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954) [M::mem_pestat] mean and std.dev: (301.52, 212.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1223) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682702 sequences (100000069 bp) in 2.408 CPU sec, 2.514 real sec... [M::process] read 685730 sequences (100000156 bp) in 1.950 CPU sec, 1.955 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 279450, 20, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 317, 610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1524) [M::mem_pestat] mean and std.dev: (367.97, 296.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1981) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 325, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949) [M::mem_pestat] mean and std.dev: (341.70, 164.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1196) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (241, 956, 1595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4303) [M::mem_pestat] mean and std.dev: (821.35, 591.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5657) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 417, 838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2152) [M::mem_pestat] mean and std.dev: (576.23, 551.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2809) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684872 reads in 178.220 CPU sec, 5.702 real sec [M::mem_process_seqs] Processed 683018 reads in 115.404 CPU sec, 3.658 real sec sorting block 123, #reads = 1069426 mem = 536880781 peak = 1145507840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 278886, 17, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 221, 632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1532) [M::mem_pestat] mean and std.dev: (357.09, 330.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1982) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 326, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955) [M::mem_pestat] mean and std.dev: (342.69, 164.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1204) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (637, 637, 707) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (497, 847) [M::mem_pestat] mean and std.dev: (666.17, 34.51) [M::mem_pestat] low and high boundaries for proper pairs: (427, 917) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 291, 652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1518) [M::mem_pestat] mean and std.dev: (350.30, 204.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1951) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685834 sequences (100000214 bp) in 2.426 CPU sec, 2.542 real sec... [M::process] read 686310 sequences (100000069 bp) in 1.940 CPU sec, 1.944 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 279627, 16, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 326, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (354.38, 187.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1155) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 303, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (323.98, 158.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1136) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (641, 1547, 2241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5441) [M::mem_pestat] mean and std.dev: (1072.47, 781.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7041) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 458, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1132) [M::mem_pestat] mean and std.dev: (361.66, 181.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1437) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682702 reads in 178.037 CPU sec, 5.654 real sec [M::mem_process_seqs] Processed 685730 reads in 117.602 CPU sec, 3.757 real sec sorting block 124, #reads = 1066247 mem = 536880893 peak = 1145507840 [M::process] read 685560 sequences (100000031 bp) in 2.053 CPU sec, 2.167 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 279734, 11, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 510, 582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1328) [M::mem_pestat] mean and std.dev: (395.68, 193.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 303, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (322.21, 156.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (327, 1368, 1766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4644) [M::mem_pestat] mean and std.dev: (983.18, 664.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6083) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 274, 633) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1577) [M::mem_pestat] mean and std.dev: (261.63, 164.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2049) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685030 sequences (100000048 bp) in 1.794 CPU sec, 1.936 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 279839, 23, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 361, 627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1441) [M::mem_pestat] mean and std.dev: (418.97, 273.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1848) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 302, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (322.50, 156.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (355, 721, 1138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2704) [M::mem_pestat] mean and std.dev: (702.75, 472.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3487) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 363, 636) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1498) [M::mem_pestat] mean and std.dev: (392.48, 233.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1929) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685834 reads in 182.743 CPU sec, 5.857 real sec sorting block 125, #reads = 1067954 mem = 536878417 peak = 1145507840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281377, 7, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 239, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (269.36, 136.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 299, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (320.06, 156.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 233, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000) [M::mem_pestat] mean and std.dev: (302.37, 170.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1267) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685028 sequences (100000051 bp) in 2.070 CPU sec, 2.074 real sec... [M::mem_process_seqs] Processed 685560 reads in 198.294 CPU sec, 6.285 real sec [M::mem_process_seqs] Processed 686310 reads in 222.053 CPU sec, 7.055 real sec sorting block 126, #reads = 1067603 mem = 536881268 peak = 1145507840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 280085, 17, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 236, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (227.00, 102.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 308, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (327.86, 159.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (559, 914, 1580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3622) [M::mem_pestat] mean and std.dev: (1031.56, 658.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4643) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 320, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988) [M::mem_pestat] mean and std.dev: (287.75, 129.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1252) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684820 sequences (100000081 bp) in 2.138 CPU sec, 2.265 real sec... [M::process] read 682854 sequences (100000269 bp) in 1.440 CPU sec, 1.440 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 279659, 7, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 352, 873) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2149) [M::mem_pestat] mean and std.dev: (572.38, 526.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2787) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 309, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (328.70, 159.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1151) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (250, 317, 542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1126) [M::mem_pestat] mean and std.dev: (357.24, 196.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1418) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685030 reads in 169.222 CPU sec, 5.387 real sec sorting block 127, #reads = 1068090 mem = 536882654 peak = 1145507840 [M::mem_process_seqs] Processed 685028 reads in 112.661 CPU sec, 3.636 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 279010, 15, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 326, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (304.80, 181.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1086) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 309, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (330.24, 161.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1163) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (200, 507, 1661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4583) [M::mem_pestat] mean and std.dev: (1094.60, 1153.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6044) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 339, 661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1631) [M::mem_pestat] mean and std.dev: (373.68, 248.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2116) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683858 sequences (100000178 bp) in 2.410 CPU sec, 2.539 real sec... [M::process] read 684806 sequences (100000292 bp) in 1.800 CPU sec, 1.804 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 279387, 10, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 332, 549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1225) [M::mem_pestat] mean and std.dev: (309.30, 135.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 326, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953) [M::mem_pestat] mean and std.dev: (342.73, 164.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1201) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (450, 681, 1082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2346) [M::mem_pestat] mean and std.dev: (880.10, 514.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2978) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 351, 664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1512) [M::mem_pestat] mean and std.dev: (393.18, 228.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1936) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684820 reads in 181.615 CPU sec, 5.804 real sec sorting block 128, #reads = 1068103 mem = 536879091 peak = 1145507840 [M::mem_process_seqs] Processed 682854 reads in 120.683 CPU sec, 3.850 real sec sorting block 129, #reads = 1066776 mem = 536880103 peak = 1145507840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 279392, 13, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (313, 344, 611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1207) [M::mem_pestat] mean and std.dev: (365.39, 164.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 317, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (335.43, 162.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (198, 817, 2838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8118) [M::mem_pestat] mean and std.dev: (1495.46, 1532.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10758) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (300, 319, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (92, 612) [M::mem_pestat] mean and std.dev: (305.66, 72.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684652 sequences (100000262 bp) in 2.412 CPU sec, 2.520 real sec... [M::process] read 684480 sequences (100000050 bp) in 1.858 CPU sec, 1.867 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 279625, 8, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 212, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (236.09, 102.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 309, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (328.94, 160.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 436, 705) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1651) [M::mem_pestat] mean and std.dev: (459.25, 249.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2124) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683858 reads in 177.413 CPU sec, 5.645 real sec [M::mem_process_seqs] Processed 684806 reads in 116.739 CPU sec, 3.694 real sec sorting block 130, #reads = 1068217 mem = 536880325 peak = 1145507840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 279808, 8, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (229, 337, 632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1438) [M::mem_pestat] mean and std.dev: (453.79, 365.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1914) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 310, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (330.11, 160.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 283, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1103) [M::mem_pestat] mean and std.dev: (302.32, 165.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1404) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684206 sequences (100000138 bp) in 2.463 CPU sec, 2.563 real sec... [M::process] read 683872 sequences (100000014 bp) in 1.879 CPU sec, 1.880 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 279454, 10, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 319, 548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1180) [M::mem_pestat] mean and std.dev: (361.34, 235.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 311, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (331.38, 161.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1168) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1138, 1436, 3048) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6868) [M::mem_pestat] mean and std.dev: (1941.60, 908.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8778) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (305, 328, 1183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2939) [M::mem_pestat] mean and std.dev: (583.27, 449.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3817) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684652 reads in 180.388 CPU sec, 5.721 real sec [M::mem_process_seqs] Processed 684480 reads in 118.814 CPU sec, 3.767 real sec sorting block 131, #reads = 1068973 mem = 536878062 peak = 1145507840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 279081, 10, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 233, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1260) [M::mem_pestat] mean and std.dev: (330.55, 227.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 312, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (333.33, 162.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1177) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (142, 661, 2458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7090) [M::mem_pestat] mean and std.dev: (1015.44, 1103.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9406) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 279, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1117) [M::mem_pestat] mean and std.dev: (372.76, 243.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1427) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684030 sequences (100000070 bp) in 2.500 CPU sec, 2.601 real sec... [M::process] read 683920 sequences (100000130 bp) in 1.960 CPU sec, 1.955 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 278545, 8, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 253, 698) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1682) [M::mem_pestat] mean and std.dev: (351.18, 264.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2174) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 314, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943) [M::mem_pestat] mean and std.dev: (335.77, 164.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1190) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 336, 560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1250) [M::mem_pestat] mean and std.dev: (367.42, 217.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1595) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684206 reads in 188.101 CPU sec, 6.195 real sec sorting block 132, #reads = 1067503 mem = 536880343 peak = 1145507840 [M::mem_process_seqs] Processed 683872 reads in 124.477 CPU sec, 4.021 real sec sorting block 133, #reads = 1065845 mem = 536879542 peak = 1145507840 [M::process] read 684274 sequences (100000141 bp) in 2.279 CPU sec, 2.380 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 279216, 17, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 317, 551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1295) [M::mem_pestat] mean and std.dev: (344.32, 256.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 313, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (334.48, 163.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1178) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (940, 1364, 4844) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12652) [M::mem_pestat] mean and std.dev: (2240.24, 2025.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16556) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 323, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1080) [M::mem_pestat] mean and std.dev: (380.41, 234.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1366) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684030 reads in 108.278 CPU sec, 3.558 real sec [M::process] read 684158 sequences (100000297 bp) in 2.377 CPU sec, 2.513 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 278565, 16, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (234, 251, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 582) [M::mem_pestat] mean and std.dev: (261.39, 94.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 311, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (334.98, 166.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1192) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (591, 1050, 1961) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4701) [M::mem_pestat] mean and std.dev: (1006.80, 717.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6071) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 296, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1111) [M::mem_pestat] mean and std.dev: (304.05, 154.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1414) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684250 sequences (100000168 bp) in 1.931 CPU sec, 1.934 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 279248, 14, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 388, 554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1236) [M::mem_pestat] mean and std.dev: (344.98, 178.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 312, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940) [M::mem_pestat] mean and std.dev: (335.79, 165.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1186) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (252, 1005, 1578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4230) [M::mem_pestat] mean and std.dev: (1234.71, 1210.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6078) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 320, 547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1213) [M::mem_pestat] mean and std.dev: (364.82, 241.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1546) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684274 reads in 213.057 CPU sec, 6.801 real sec sorting block 134, #reads = 1064415 mem = 536878660 peak = 1145507840 [M::mem_process_seqs] Processed 683920 reads in 234.645 CPU sec, 7.506 real sec sorting block 135, #reads = 1063083 mem = 536883361 peak = 1145507840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 279118, 13, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 359, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024) [M::mem_pestat] mean and std.dev: (338.10, 207.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1291) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 313, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948) [M::mem_pestat] mean and std.dev: (336.21, 166.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1197) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (127, 519, 914) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2488) [M::mem_pestat] mean and std.dev: (568.92, 558.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3275) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 275, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (258.57, 99.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684080 sequences (100000008 bp) in 2.399 CPU sec, 2.525 real sec... [M::process] read 684772 sequences (100000024 bp) in 1.737 CPU sec, 1.731 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 278926, 15, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 357, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1069) [M::mem_pestat] mean and std.dev: (309.24, 159.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1366) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 309, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (334.34, 166.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1188) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (212, 303, 1822) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5042) [M::mem_pestat] mean and std.dev: (892.86, 1173.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6652) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 276, 631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1457) [M::mem_pestat] mean and std.dev: (447.70, 326.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1870) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684158 reads in 186.983 CPU sec, 5.944 real sec [M::mem_process_seqs] Processed 684250 reads in 128.154 CPU sec, 4.077 real sec sorting block 136, #reads = 1063935 mem = 536878445 peak = 1145507840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278794, 10, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 220, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (243.59, 129.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 308, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (333.34, 166.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1180) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1, 72, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1348) [M::mem_pestat] mean and std.dev: (96.25, 144.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1797) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (261, 320, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (318.58, 111.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687978 sequences (100000175 bp) in 2.454 CPU sec, 2.548 real sec... [M::process] read 690444 sequences (100000189 bp) in 1.910 CPU sec, 1.906 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 279171, 12, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (244, 331, 749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1759) [M::mem_pestat] mean and std.dev: (479.26, 394.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2264) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 302, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (328.35, 164.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (126, 317, 2665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7743) [M::mem_pestat] mean and std.dev: (863.33, 1119.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10282) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 408, 688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1622) [M::mem_pestat] mean and std.dev: (436.91, 322.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2089) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684080 reads in 194.120 CPU sec, 6.193 real sec [M::mem_process_seqs] Processed 684772 reads in 132.832 CPU sec, 4.255 real sec sorting block 137, #reads = 1061927 mem = 536878610 peak = 1145507840 [M::process] read 688674 sequences (100000176 bp) in 2.430 CPU sec, 2.509 real sec... [M::process] read 689928 sequences (100000066 bp) in 1.980 CPU sec, 1.980 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 280325, 9, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 452, 547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1311) [M::mem_pestat] mean and std.dev: (348.23, 227.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 290, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (311.97, 152.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 349, 2567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7219) [M::mem_pestat] mean and std.dev: (1145.84, 1487.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9545) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688674 reads in 120.807 CPU sec, 3.837 real sec sorting block 138, #reads = 1058225 mem = 536880541 peak = 1145507840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 279497, 12, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 256, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (279.28, 177.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 292, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (317.25, 159.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (292, 1098, 1798) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4810) [M::mem_pestat] mean and std.dev: (802.50, 623.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6316) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 313, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1122) [M::mem_pestat] mean and std.dev: (311.73, 140.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1424) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 281159, 15, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 316, 694) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1640) [M::mem_pestat] mean and std.dev: (329.65, 206.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2113) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 285, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (307.79, 152.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (245, 334, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (296.62, 116.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (281, 479, 668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1442) [M::mem_pestat] mean and std.dev: (519.08, 380.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2040) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688760 sequences (100000230 bp) in 2.178 CPU sec, 2.186 real sec... [M::mem_process_seqs] Processed 687978 reads in 323.731 CPU sec, 10.277 real sec [M::mem_process_seqs] Processed 690444 reads in 255.200 CPU sec, 8.113 real sec sorting block 139, #reads = 1054461 mem = 536878719 peak = 1145507840 [M::process] read 685884 sequences (100000086 bp) in 2.389 CPU sec, 2.482 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 281361, 7, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 310, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1123) [M::mem_pestat] mean and std.dev: (313.54, 174.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 283, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (305.39, 150.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1091) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 323, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797) [M::mem_pestat] mean and std.dev: (323.20, 136.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 989) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687758 sequences (100000045 bp) in 1.496 CPU sec, 1.495 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 280743, 12, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 308, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1181) [M::mem_pestat] mean and std.dev: (338.53, 228.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 287, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (308.96, 151.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (606, 1494, 5743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16017) [M::mem_pestat] mean and std.dev: (2158.83, 2122.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21154) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (269, 388, 671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1475) [M::mem_pestat] mean and std.dev: (449.64, 280.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1877) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 280276, 8, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (277, 347, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (15, 670) [M::mem_pestat] mean and std.dev: (323.98, 113.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 312, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (329.49, 159.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1178) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (238, 302, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015) [M::mem_pestat] mean and std.dev: (321.67, 149.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1274) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689928 reads in 275.402 CPU sec, 8.803 real sec sorting block 140, #reads = 1059176 mem = 536883685 peak = 1145507840 [M::mem_process_seqs] Processed 688760 reads in 217.116 CPU sec, 6.974 real sec [M::mem_process_seqs] Processed 685884 reads in 232.297 CPU sec, 7.463 real sec [M::process] read 687736 sequences (100000004 bp) in 2.426 CPU sec, 2.581 real sec... sorting block 141, #reads = 1060597 mem = 536880328 peak = 1145507840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 280609, 15, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 442, 926) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2304) [M::mem_pestat] mean and std.dev: (528.95, 395.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2993) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 293, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (315.05, 154.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (606, 1018, 1412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3024) [M::mem_pestat] mean and std.dev: (745.58, 444.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3830) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 342, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1195) [M::mem_pestat] mean and std.dev: (323.06, 149.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1516) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688902 sequences (100000094 bp) in 1.987 CPU sec, 2.077 real sec... [M::process] read 688788 sequences (100000213 bp) in 1.430 CPU sec, 1.432 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 280673, 16, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 333, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1168) [M::mem_pestat] mean and std.dev: (328.14, 170.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 294, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (316.89, 156.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (661, 826, 1105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1993) [M::mem_pestat] mean and std.dev: (845.00, 506.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2872) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 219, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (293.88, 169.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1097) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687758 reads in 189.033 CPU sec, 6.022 real sec [M::mem_process_seqs] Processed 687736 reads in 120.378 CPU sec, 3.821 real sec sorting block 142, #reads = 1061580 mem = 536880207 peak = 1145507840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 280816, 16, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 251, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (267.47, 142.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 286, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (309.22, 152.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (301, 422, 999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2395) [M::mem_pestat] mean and std.dev: (646.67, 612.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3097) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 349, 729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1857) [M::mem_pestat] mean and std.dev: (389.00, 317.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2421) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687456 sequences (100000260 bp) in 2.454 CPU sec, 2.573 real sec... [M::process] read 686652 sequences (100000038 bp) in 1.790 CPU sec, 1.793 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281148, 8, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (243, 273, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (7, 597) [M::mem_pestat] mean and std.dev: (267.83, 99.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 286, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (306.20, 148.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1077) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 444, 721) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1791) [M::mem_pestat] mean and std.dev: (462.43, 323.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2326) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688902 reads in 188.808 CPU sec, 6.072 real sec sorting block 143, #reads = 1065013 mem = 536877799 peak = 1145507840 [M::mem_process_seqs] Processed 688788 reads in 127.968 CPU sec, 4.082 real sec sorting block 144, #reads = 1063957 mem = 536878592 peak = 1145507840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 280662, 10, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 285, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (284.23, 167.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 292, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (312.63, 151.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (551, 845, 1486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3356) [M::mem_pestat] mean and std.dev: (873.78, 526.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4291) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 354, 541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1213) [M::mem_pestat] mean and std.dev: (333.75, 170.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1549) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685082 sequences (100000106 bp) in 2.309 CPU sec, 2.389 real sec... [M::process] read 684966 sequences (100000083 bp) in 1.540 CPU sec, 1.538 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 280870, 9, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 294, 549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1253) [M::mem_pestat] mean and std.dev: (366.96, 239.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 295, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (315.61, 152.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1104) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (253, 314, 776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1822) [M::mem_pestat] mean and std.dev: (408.13, 315.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2345) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687456 reads in 178.563 CPU sec, 5.676 real sec [M::mem_process_seqs] Processed 686652 reads in 113.774 CPU sec, 3.836 real sec sorting block 145, #reads = 1064354 mem = 536880845 peak = 1145507840 [M::process] read 686032 sequences (100000238 bp) in 2.042 CPU sec, 2.161 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 279064, 11, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 240, 603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1439) [M::mem_pestat] mean and std.dev: (353.32, 259.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1857) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 309, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (326.57, 156.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1143) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (89, 591, 2633) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7721) [M::mem_pestat] mean and std.dev: (1025.36, 1102.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10265) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (249, 369, 784) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1854) [M::mem_pestat] mean and std.dev: (489.55, 369.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2389) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685892 sequences (100000189 bp) in 1.456 CPU sec, 1.463 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 279588, 10, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (229, 330, 546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1180) [M::mem_pestat] mean and std.dev: (339.88, 177.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 309, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (326.44, 155.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 460, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1383) [M::mem_pestat] mean and std.dev: (258.00, 212.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1831) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 263, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (306.51, 181.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1089) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 279649, 7, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 314, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1373) [M::mem_pestat] mean and std.dev: (327.94, 206.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 301, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (320.84, 154.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (275, 279, 605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1265) [M::mem_pestat] mean and std.dev: (305.88, 153.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1595) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685082 reads in 260.322 CPU sec, 8.567 real sec [M::mem_process_seqs] Processed 684966 reads in 196.975 CPU sec, 6.345 real sec sorting block 146, #reads = 1064885 mem = 536881720 peak = 1145507840 [M::mem_process_seqs] Processed 686032 reads in 215.308 CPU sec, 6.955 real sec sorting block 147, #reads = 1063961 mem = 536879775 peak = 1145507840 [M::process] read 684708 sequences (100000116 bp) in 2.650 CPU sec, 2.835 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 279509, 11, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 421, 601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1437) [M::mem_pestat] mean and std.dev: (323.58, 154.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1855) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 300, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (319.23, 153.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (403, 1054, 2256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5962) [M::mem_pestat] mean and std.dev: (1283.90, 1605.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7815) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 347, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019) [M::mem_pestat] mean and std.dev: (318.19, 158.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1296) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685892 reads in 104.737 CPU sec, 3.422 real sec [M::process] read 685182 sequences (100000073 bp) in 2.025 CPU sec, 2.268 real sec... sorting block 148, #reads = 1064819 mem = 536881091 peak = 1145507840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 278822, 4, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 279, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978) [M::mem_pestat] mean and std.dev: (253.40, 119.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1248) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 309, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (327.04, 156.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 442, 789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1981) [M::mem_pestat] mean and std.dev: (562.33, 566.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2827) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685246 sequences (100000293 bp) in 1.606 CPU sec, 1.621 real sec... [M::process] read 684550 sequences (100000186 bp) in 1.350 CPU sec, 1.355 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 279441, 22, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 223, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (224.78, 111.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 305, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (324.75, 156.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (291, 411, 535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023) [M::mem_pestat] mean and std.dev: (343.67, 164.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1267) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 206, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (220.00, 77.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684708 reads in 197.234 CPU sec, 6.294 real sec [M::mem_process_seqs] Processed 685182 reads in 113.502 CPU sec, 3.681 real sec sorting block 149, #reads = 1064923 mem = 536880148 peak = 1145507840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 279252, 7, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 352, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (325.00, 162.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 305, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (325.01, 157.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 446, 1332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3626) [M::mem_pestat] mean and std.dev: (641.58, 708.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4773) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684650 sequences (100000130 bp) in 2.317 CPU sec, 2.415 real sec... [M::process] read 684372 sequences (100000069 bp) in 1.710 CPU sec, 1.711 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278833, 11, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 259, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962) [M::mem_pestat] mean and std.dev: (298.08, 211.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1237) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 309, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (329.60, 160.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (160, 568, 1073) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2899) [M::mem_pestat] mean and std.dev: (428.78, 362.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3812) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 391, 657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1511) [M::mem_pestat] mean and std.dev: (447.98, 308.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1938) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685246 reads in 170.883 CPU sec, 5.501 real sec [M::mem_process_seqs] Processed 684550 reads in 112.119 CPU sec, 3.660 real sec sorting block 150, #reads = 1065395 mem = 536878073 peak = 1145507840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 278409, 15, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 302, 766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1916) [M::mem_pestat] mean and std.dev: (480.88, 432.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 684174 sequences (100000003 bp) in 2.113 CPU sec, 2.262 real sec... [M::mem_pestat] (25, 50, 75) percentile: (198, 307, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (327.09, 158.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (394, 484, 1391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3385) [M::mem_pestat] mean and std.dev: (707.64, 515.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4382) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (259, 358, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937) [M::mem_pestat] mean and std.dev: (335.66, 149.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1163) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684126 sequences (100000233 bp) in 1.530 CPU sec, 1.523 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 279071, 10, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (244, 335, 546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1150) [M::mem_pestat] mean and std.dev: (310.81, 143.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 310, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (330.41, 160.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (66, 716, 3503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10377) [M::mem_pestat] mean and std.dev: (1283.20, 1475.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13814) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 325, 856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2214) [M::mem_pestat] mean and std.dev: (403.54, 293.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2893) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684650 reads in 165.038 CPU sec, 5.347 real sec sorting block 151, #reads = 1065197 mem = 536878123 peak = 1145507840 [M::mem_process_seqs] Processed 684372 reads in 108.436 CPU sec, 3.461 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278613, 12, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 356, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1140) [M::mem_pestat] mean and std.dev: (327.37, 177.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 309, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (330.51, 160.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (252, 866, 1761) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4779) [M::mem_pestat] mean and std.dev: (1170.00, 1178.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6288) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 310, 588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1390) [M::mem_pestat] mean and std.dev: (368.96, 227.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1791) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683916 sequences (100000085 bp) in 2.176 CPU sec, 2.334 real sec... [M::process] read 683464 sequences (100000230 bp) in 1.520 CPU sec, 1.518 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 278794, 16, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 244, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1120) [M::mem_pestat] mean and std.dev: (289.54, 168.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 310, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (332.05, 162.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (325, 856, 1284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3202) [M::mem_pestat] mean and std.dev: (788.94, 623.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4161) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 326, 605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1365) [M::mem_pestat] mean and std.dev: (297.72, 113.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1745) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684174 reads in 160.547 CPU sec, 5.113 real sec sorting block 152, #reads = 1065341 mem = 536878374 peak = 1145507840 [M::mem_process_seqs] Processed 684126 reads in 110.311 CPU sec, 3.520 real sec sorting block 153, #reads = 1065317 mem = 536880592 peak = 1145507840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 278649, 11, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (277, 459, 678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1480) [M::mem_pestat] mean and std.dev: (538.35, 377.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2048) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 311, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (333.34, 163.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1170) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (200, 606, 1295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3485) [M::mem_pestat] mean and std.dev: (726.55, 596.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4580) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 274, 743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1901) [M::mem_pestat] mean and std.dev: (464.52, 397.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2480) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 563032 sequences (82184985 bp) in 2.015 CPU sec, 2.137 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 278117, 29, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 233, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (311.55, 216.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1182) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 314, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (336.33, 164.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1183) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (698, 903, 1081) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1847) [M::mem_pestat] mean and std.dev: (726.12, 326.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2230) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (245, 309, 574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1232) [M::mem_pestat] mean and std.dev: (389.39, 229.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1561) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683916 reads in 157.190 CPU sec, 5.066 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 229865, 11, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 248, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1186) [M::mem_pestat] mean and std.dev: (286.61, 202.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 301, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (326.94, 163.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (945, 1705, 2909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6837) [M::mem_pestat] mean and std.dev: (1884.73, 1386.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8801) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 350, 515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1205) [M::mem_pestat] mean and std.dev: (325.29, 151.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1550) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 563032 reads in 72.611 CPU sec, 2.501 real sec sorting block 154, #reads = 1065398 mem = 536880041 peak = 1145507840 [M::mem_process_seqs] Processed 683464 reads in 175.785 CPU sec, 6.392 real sec sorting block 155, #reads = 1063270 mem = 536879090 peak = 1238245376 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 165288822 overall: 70310457344 mem 25546.564 user 619.491 sys 867.305 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_253-T1-TDNA-1_A23MHWWLT4_1.L008\tPU:L008\tSM:1136_253-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_253-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1136_253-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 1136_253-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 867.347 sec; CPU: 26166.063 sec sorting block 156, #reads = 79016 mem = 39979927 peak = 1238245376 uniq header count 1 merging 157 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 9687869661 output reads: 166564323 bam_mem_sort: 157 calls 88.336 user 0.953 sys 90.941 real bam_write: 157 calls 61.461 user 3.155 sys 86.729 real execute: 1 calls 34.050 user 1.433 sys 921.537 real merge_files: 1 calls 34.043 user 1.391 sys 54.143 real parse_chunk: 2498 calls 106.016 user 28.336 sys 144.992 real read_chunk: 6744 calls 52.273 user 52.164 sys 811.072 real sort_block: 1 calls 35.314 user 51.499 sys 787.243 real write_chunk: 4244 calls 33.698 user 1.075 sys 53.201 real overall: 3453059072 mem 2164.220 user 101.823 sys 921.555 real ls: cannot access '*.cram': No such file or directory