This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_cVw-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1788.511 CPU sec, 65.440 real sec
[M::process] read 679640 sequences (100000279 bp) in 1.391 CPU sec, 1.394 real sec...
[M::process] read 679282 sequences (100000241 bp) in 1.890 CPU sec, 1.892 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283607, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 324, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (340.76, 158.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678822 sequences (100000125 bp) in 1.867 CPU sec, 1.984 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282990, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 327, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (343.14, 159.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678006 sequences (100000125 bp) in 1.817 CPU sec, 1.966 real sec...
[M::mem_process_seqs] Processed 679640 reads in 189.011 CPU sec, 6.197 real sec
[M::mem_process_seqs] Processed 679282 reads in 122.269 CPU sec, 4.141 real sec
sorting block 0, #reads = 1083042 mem = 536879385 peak = 586928128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283373, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 332, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (346.95, 159.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677934 sequences (100000060 bp) in 2.611 CPU sec, 2.881 real sec...
[M::process] read 677862 sequences (100000090 bp) in 1.876 CPU sec, 2.089 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282469, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 335, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (351.02, 161.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678822 reads in 186.332 CPU sec, 6.279 real sec
[M::mem_process_seqs] Processed 678006 reads in 123.713 CPU sec, 4.631 real sec
sorting block 1, #reads = 1083191 mem = 536881203 peak = 714330112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283004, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 339, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (354.24, 163.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677674 sequences (100000139 bp) in 2.077 CPU sec, 2.271 real sec...
[M::process] read 677540 sequences (100000278 bp) in 1.977 CPU sec, 2.051 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282496, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 340, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (354.63, 163.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677934 reads in 187.652 CPU sec, 6.641 real sec
[M::mem_process_seqs] Processed 677862 reads in 121.233 CPU sec, 4.567 real sec
sorting block 2, #reads = 1082809 mem = 536879306 peak = 829566976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282660, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 342, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (356.48, 163.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677362 sequences (100000217 bp) in 2.739 CPU sec, 3.537 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282687, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 341, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (355.24, 162.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677674 reads in 187.266 CPU sec, 6.679 real sec
[M::process] read 677040 sequences (100000293 bp) in 2.021 CPU sec, 2.291 real sec...
[M::mem_process_seqs] Processed 677540 reads in 122.942 CPU sec, 4.657 real sec
sorting block 3, #reads = 1083754 mem = 536878260 peak = 864694272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282567, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 342, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (356.25, 162.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676914 sequences (100000217 bp) in 2.634 CPU sec, 2.951 real sec...
sorting block 4, #reads = 1083666 mem = 536881921 peak = 864694272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282163, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 345, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (358.48, 162.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676822 sequences (100000136 bp) in 1.916 CPU sec, 2.012 real sec...
[M::mem_process_seqs] Processed 677362 reads in 188.885 CPU sec, 6.969 real sec
[M::mem_process_seqs] Processed 677040 reads in 122.887 CPU sec, 4.918 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 282458, 2, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (964, 1560, 1560)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2752)
[M::mem_pestat] mean and std.dev: (1361.33, 280.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3348)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 347, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (360.25, 163.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 5, #reads = 1083456 mem = 536880789 peak = 864694272
[M::process] read 676450 sequences (100000289 bp) in 3.087 CPU sec, 3.559 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283251, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 350, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (363.38, 165.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676914 reads in 177.339 CPU sec, 5.921 real sec
[M::mem_process_seqs] Processed 676822 reads in 119.883 CPU sec, 4.422 real sec
[M::process] read 677454 sequences (100000003 bp) in 2.617 CPU sec, 3.002 real sec...
sorting block 6, #reads = 1082623 mem = 536877469 peak = 864694272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282154, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 352, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (364.75, 165.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677876 sequences (100000202 bp) in 2.582 CPU sec, 3.176 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282638, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 346, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (359.33, 164.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676450 reads in 181.516 CPU sec, 6.463 real sec
[M::mem_process_seqs] Processed 677454 reads in 122.572 CPU sec, 4.321 real sec
[M::process] read 677390 sequences (100000251 bp) in 2.686 CPU sec, 2.816 real sec...
sorting block 7, #reads = 1082522 mem = 536877530 peak = 864694272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281982, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 341, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (355.71, 164.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677420 sequences (100000021 bp) in 2.548 CPU sec, 2.864 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282445, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 343, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (357.42, 164.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677876 reads in 183.135 CPU sec, 6.491 real sec
[M::process] read 677252 sequences (100000236 bp) in 2.251 CPU sec, 2.391 real sec...
[M::mem_process_seqs] Processed 677390 reads in 123.060 CPU sec, 4.690 real sec
sorting block 8, #reads = 1084071 mem = 536878591 peak = 864694272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281817, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 342, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (356.75, 163.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677148 sequences (100000053 bp) in 2.684 CPU sec, 3.261 real sec...
sorting block 9, #reads = 1082945 mem = 536879054 peak = 880742400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281601, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 341, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (357.10, 163.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677420 reads in 182.481 CPU sec, 6.179 real sec
[M::mem_process_seqs] Processed 677252 reads in 123.996 CPU sec, 4.164 real sec
[M::process] read 677008 sequences (100000280 bp) in 2.435 CPU sec, 2.669 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281983, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 342, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (357.90, 164.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 10, #reads = 1081969 mem = 536879151 peak = 880742400
[M::process] read 677168 sequences (100000029 bp) in 2.908 CPU sec, 3.685 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282030, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 342, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (356.56, 162.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677148 reads in 184.569 CPU sec, 6.489 real sec
[M::mem_process_seqs] Processed 677008 reads in 125.486 CPU sec, 4.543 real sec
[M::process] read 676926 sequences (100000214 bp) in 2.479 CPU sec, 2.725 real sec...
sorting block 11, #reads = 1082801 mem = 536879864 peak = 880742400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282053, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 340, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (355.60, 162.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676996 sequences (100000263 bp) in 2.889 CPU sec, 3.523 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282004, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 340, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (355.43, 162.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677168 reads in 182.223 CPU sec, 6.093 real sec
[M::mem_process_seqs] Processed 676926 reads in 121.844 CPU sec, 4.104 real sec
[M::process] read 676866 sequences (100000156 bp) in 2.734 CPU sec, 2.975 real sec...
sorting block 12, #reads = 1083509 mem = 536880021 peak = 880742400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281767, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 338, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (353.06, 160.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282290, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 338, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (352.82, 159.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677110 sequences (100000133 bp) in 3.032 CPU sec, 3.398 real sec...
[M::mem_process_seqs] Processed 676996 reads in 180.866 CPU sec, 6.194 real sec
[M::mem_process_seqs] Processed 676866 reads in 121.999 CPU sec, 4.211 real sec
sorting block 13, #reads = 1081920 mem = 536878677 peak = 880742400
[M::process] read 678040 sequences (100000008 bp) in 2.602 CPU sec, 3.001 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281697, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 332, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (348.34, 157.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282763, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 324, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (340.01, 153.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1111)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 14, #reads = 1083185 mem = 536877555 peak = 880742400
[M::process] read 679104 sequences (100000221 bp) in 2.845 CPU sec, 3.709 real sec...
[M::mem_process_seqs] Processed 677110 reads in 186.199 CPU sec, 6.431 real sec
[M::mem_process_seqs] Processed 678040 reads in 126.743 CPU sec, 4.882 real sec
[M::process] read 677498 sequences (100000270 bp) in 2.666 CPU sec, 3.101 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283258, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 320, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881)
[M::mem_pestat] mean and std.dev: (335.12, 151.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1103)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 15, #reads = 1081454 mem = 536880495 peak = 880742400
[M::process] read 677416 sequences (100000248 bp) in 2.846 CPU sec, 3.416 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282042, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 331, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (347.03, 157.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679104 reads in 197.738 CPU sec, 6.764 real sec
[M::mem_process_seqs] Processed 677498 reads in 128.790 CPU sec, 4.336 real sec
[M::process] read 677226 sequences (100000024 bp) in 2.641 CPU sec, 2.889 real sec...
sorting block 16, #reads = 1078344 mem = 536882483 peak = 880742400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282267, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 331, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (346.70, 157.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677038 sequences (100000219 bp) in 2.911 CPU sec, 3.353 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281738, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 330, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (347.67, 158.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677416 reads in 187.649 CPU sec, 6.328 real sec
[M::mem_process_seqs] Processed 677226 reads in 126.331 CPU sec, 4.188 real sec
sorting block 17, #reads = 1081801 mem = 536879638 peak = 880742400
[M::process] read 677188 sequences (100000019 bp) in 2.817 CPU sec, 3.000 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282252, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 333, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (349.32, 159.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282224, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 677212 sequences (100000227 bp) in 3.086 CPU sec, 3.480 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (347.51, 158.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677038 reads in 181.741 CPU sec, 5.938 real sec
sorting block 18, #reads = 1082374 mem = 536877091 peak = 880742400
[M::mem_process_seqs] Processed 677188 reads in 122.882 CPU sec, 4.025 real sec
[M::process] read 676958 sequences (100000295 bp) in 2.787 CPU sec, 3.053 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281908, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (347.24, 158.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 19, #reads = 1082571 mem = 536881101 peak = 880742400
[M::process] read 676906 sequences (100000174 bp) in 2.674 CPU sec, 2.989 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282744, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 332, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (349.12, 158.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677212 reads in 178.735 CPU sec, 5.782 real sec
[M::mem_process_seqs] Processed 676958 reads in 119.535 CPU sec, 3.819 real sec
[M::process] read 676848 sequences (100000063 bp) in 3.026 CPU sec, 3.304 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282193, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 336, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (351.70, 160.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 20, #reads = 1082508 mem = 536875648 peak = 880742400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282019, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 333, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (349.92, 159.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676714 sequences (100000196 bp) in 2.785 CPU sec, 2.986 real sec...
[M::mem_process_seqs] Processed 676906 reads in 176.044 CPU sec, 5.690 real sec
[M::mem_process_seqs] Processed 676848 reads in 116.170 CPU sec, 3.708 real sec
sorting block 21, #reads = 1081837 mem = 536879605 peak = 880742400
[M::process] read 676668 sequences (100000192 bp) in 2.983 CPU sec, 3.164 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281746, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 333, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (350.11, 158.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281675, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 334, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (350.40, 158.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676388 sequences (100000180 bp) in 2.871 CPU sec, 2.996 real sec...
[M::mem_process_seqs] Processed 676714 reads in 170.688 CPU sec, 5.469 real sec
[M::mem_process_seqs] Processed 676668 reads in 114.661 CPU sec, 3.633 real sec
sorting block 22, #reads = 1081799 mem = 536878611 peak = 880742400
[M::process] read 676734 sequences (100000195 bp) in 3.094 CPU sec, 3.232 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282564, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 335, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (352.16, 160.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282374, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 336, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (351.99, 159.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678746 sequences (100000036 bp) in 2.614 CPU sec, 2.684 real sec...
[M::mem_process_seqs] Processed 676388 reads in 166.038 CPU sec, 5.298 real sec
sorting block 23, #reads = 1083344 mem = 536883909 peak = 880742400
[M::mem_process_seqs] Processed 676734 reads in 108.981 CPU sec, 3.432 real sec
[M::process] read 678362 sequences (100000094 bp) in 2.950 CPU sec, 3.089 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283124, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 328, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (344.62, 159.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 24, #reads = 1082160 mem = 536882473 peak = 880742400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283202, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 331, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (347.45, 160.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678314 sequences (100000204 bp) in 2.422 CPU sec, 2.489 real sec...
[M::mem_process_seqs] Processed 678746 reads in 159.645 CPU sec, 5.068 real sec
[M::mem_process_seqs] Processed 678362 reads in 108.334 CPU sec, 3.423 real sec
[M::process] read 678120 sequences (100000247 bp) in 2.928 CPU sec, 3.060 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282684, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 333, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (349.42, 161.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 25, #reads = 1082344 mem = 536879137 peak = 880742400
[M::process] read 677708 sequences (100000058 bp) in 2.343 CPU sec, 2.417 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283156, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 335, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (350.95, 162.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678314 reads in 160.778 CPU sec, 5.131 real sec
[M::mem_process_seqs] Processed 678120 reads in 106.693 CPU sec, 3.368 real sec
sorting block 26, #reads = 1084371 mem = 536883432 peak = 880742400
[M::process] read 677540 sequences (100000243 bp) in 2.587 CPU sec, 2.694 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282392, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 341, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (356.49, 164.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677212 sequences (100000293 bp) in 2.544 CPU sec, 2.606 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282821, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 344, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (358.22, 165.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677708 reads in 158.096 CPU sec, 5.017 real sec
sorting block 27, #reads = 1084831 mem = 536877963 peak = 880742400
[M::process] read 677204 sequences (100000138 bp) in 2.413 CPU sec, 2.459 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282555, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 345, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (360.53, 166.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677154 sequences (100000237 bp) in 1.443 CPU sec, 1.449 real sec...
[M::mem_process_seqs] Processed 677212 reads in 90.129 CPU sec, 2.853 real sec
[M::mem_process_seqs] Processed 677540 reads in 215.984 CPU sec, 6.820 real sec
sorting block 28, #reads = 1084521 mem = 536878259 peak = 880742400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282796, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 346, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (361.52, 167.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676968 sequences (100000059 bp) in 2.515 CPU sec, 2.579 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282537, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 347, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (360.65, 165.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677204 reads in 168.438 CPU sec, 5.330 real sec
[M::mem_process_seqs] Processed 677154 reads in 106.356 CPU sec, 3.343 real sec
[M::process] read 676478 sequences (100000210 bp) in 2.378 CPU sec, 2.394 real sec...
sorting block 29, #reads = 1083284 mem = 536879478 peak = 880742400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282267, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 348, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (362.03, 165.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676492 sequences (100000131 bp) in 2.409 CPU sec, 2.463 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281851, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 352, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (364.89, 165.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676968 reads in 149.029 CPU sec, 4.712 real sec
[M::process] read 676452 sequences (100000015 bp) in 1.986 CPU sec, 1.998 real sec...
sorting block 30, #reads = 1083206 mem = 536877685 peak = 895758336
[M::mem_process_seqs] Processed 676478 reads in 99.193 CPU sec, 3.121 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282093, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 355, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (367.39, 167.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 31, #reads = 1082987 mem = 536878023 peak = 921083904
[M::process] read 676388 sequences (100000052 bp) in 2.578 CPU sec, 2.637 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281840, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 354, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (367.49, 167.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676492 reads in 148.290 CPU sec, 4.693 real sec
[M::process] read 677216 sequences (100000132 bp) in 1.860 CPU sec, 1.868 real sec...
[M::mem_process_seqs] Processed 676452 reads in 95.564 CPU sec, 2.999 real sec
sorting block 32, #reads = 1083939 mem = 536878937 peak = 921083904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 281549, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 354, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (367.97, 167.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677320 sequences (100000119 bp) in 2.324 CPU sec, 2.391 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282259, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 345, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (360.61, 166.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677094 sequences (100000018 bp) in 1.600 CPU sec, 1.599 real sec...
[M::mem_process_seqs] Processed 676388 reads in 149.024 CPU sec, 4.717 real sec
[M::mem_process_seqs] Processed 677216 reads in 97.467 CPU sec, 3.077 real sec
sorting block 33, #reads = 1082788 mem = 536878619 peak = 921083904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282842, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 344, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (359.20, 165.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677222 sequences (100000120 bp) in 2.597 CPU sec, 2.660 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282427, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 343, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (359.04, 165.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677004 sequences (100000195 bp) in 1.590 CPU sec, 1.594 real sec...
[M::mem_process_seqs] Processed 677320 reads in 141.571 CPU sec, 4.467 real sec
sorting block 34, #reads = 1083227 mem = 536880860 peak = 924233728
[M::mem_process_seqs] Processed 677094 reads in 94.523 CPU sec, 2.979 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282082, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 341, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (357.91, 165.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677026 sequences (100000210 bp) in 2.277 CPU sec, 2.338 real sec...
[M::process] read 677040 sequences (100000013 bp) in 1.550 CPU sec, 1.556 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282175, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 343, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (358.31, 164.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677222 reads in 147.468 CPU sec, 4.666 real sec
sorting block 35, #reads = 1084131 mem = 536880185 peak = 924233728
[M::mem_process_seqs] Processed 677004 reads in 97.412 CPU sec, 3.095 real sec
sorting block 36, #reads = 1082755 mem = 536881157 peak = 987148288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282327, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 341, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (357.51, 164.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677280 sequences (100000266 bp) in 2.408 CPU sec, 2.469 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282170, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 338, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (354.84, 163.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677292 sequences (100000148 bp) in 1.600 CPU sec, 1.599 real sec...
[M::mem_process_seqs] Processed 677026 reads in 144.023 CPU sec, 4.554 real sec
[M::mem_process_seqs] Processed 677040 reads in 93.070 CPU sec, 2.938 real sec
sorting block 37, #reads = 1082799 mem = 536879244 peak = 987148288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282540, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 338, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (354.57, 163.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677564 sequences (100000089 bp) in 2.810 CPU sec, 2.868 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282542, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 335, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (352.00, 161.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677280 reads in 145.938 CPU sec, 4.604 real sec
[M::mem_process_seqs] Processed 677292 reads in 96.351 CPU sec, 3.020 real sec
[M::process] read 677792 sequences (100000285 bp) in 2.208 CPU sec, 2.212 real sec...
sorting block 38, #reads = 1083285 mem = 536879155 peak = 987148288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282269, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 333, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (350.31, 161.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677504 sequences (100000213 bp) in 2.284 CPU sec, 2.364 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282327, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 333, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (350.45, 161.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678814 sequences (100000076 bp) in 1.660 CPU sec, 1.651 real sec...
[M::mem_process_seqs] Processed 677564 reads in 142.256 CPU sec, 4.501 real sec
[M::mem_process_seqs] Processed 677792 reads in 95.656 CPU sec, 3.022 real sec
sorting block 39, #reads = 1082974 mem = 536877589 peak = 987148288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281980, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 333, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (350.04, 161.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678122 sequences (100000100 bp) in 2.587 CPU sec, 2.676 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282834, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 326, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (341.70, 157.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677368 sequences (100000189 bp) in 1.690 CPU sec, 1.694 real sec...
[M::mem_process_seqs] Processed 677504 reads in 152.906 CPU sec, 4.835 real sec
sorting block 40, #reads = 1082499 mem = 536882268 peak = 987148288
[M::mem_process_seqs] Processed 678814 reads in 106.094 CPU sec, 3.351 real sec
sorting block 41, #reads = 1080429 mem = 536880692 peak = 987148288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282599, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 328, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (344.92, 158.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677624 sequences (100000289 bp) in 2.823 CPU sec, 2.887 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282113, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 333, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (350.08, 161.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678122 reads in 153.269 CPU sec, 4.834 real sec
[M::mem_process_seqs] Processed 677368 reads in 103.843 CPU sec, 3.258 real sec
[M::process] read 677192 sequences (100000177 bp) in 2.505 CPU sec, 2.524 real sec...
sorting block 42, #reads = 1079634 mem = 536878555 peak = 987148288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282530, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 334, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (350.63, 161.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677228 sequences (100000271 bp) in 2.698 CPU sec, 2.776 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282387, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 333, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (351.44, 162.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677624 reads in 153.534 CPU sec, 4.855 real sec
[M::mem_process_seqs] Processed 677192 reads in 100.749 CPU sec, 3.161 real sec
[M::process] read 677188 sequences (100000067 bp) in 2.287 CPU sec, 2.303 real sec...
sorting block 43, #reads = 1082784 mem = 536877091 peak = 987148288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282388, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 333, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (349.44, 160.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677442 sequences (100000030 bp) in 2.574 CPU sec, 2.636 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282556, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 334, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (350.52, 160.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677228 reads in 147.889 CPU sec, 4.680 real sec
[M::mem_process_seqs] Processed 677188 reads in 97.514 CPU sec, 3.064 real sec
[M::process] read 677030 sequences (100000158 bp) in 2.089 CPU sec, 2.093 real sec...
sorting block 44, #reads = 1082649 mem = 536878474 peak = 987148288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282491, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 332, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (349.56, 160.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676914 sequences (100000196 bp) in 2.392 CPU sec, 2.462 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282413, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 333, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (350.00, 159.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676846 sequences (100000143 bp) in 1.630 CPU sec, 1.630 real sec...
[M::mem_process_seqs] Processed 677442 reads in 148.062 CPU sec, 4.688 real sec
sorting block 45, #reads = 1082149 mem = 536879705 peak = 987148288
[M::mem_process_seqs] Processed 677030 reads in 97.511 CPU sec, 3.076 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282072, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 333, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (349.96, 159.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 46, #reads = 1082727 mem = 536878654 peak = 1004859392
[M::process] read 676560 sequences (100000204 bp) in 2.810 CPU sec, 2.879 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282123, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 334, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (350.48, 160.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676914 reads in 145.504 CPU sec, 4.613 real sec
[M::mem_process_seqs] Processed 676846 reads in 97.656 CPU sec, 3.065 real sec
[M::process] read 676712 sequences (100000195 bp) in 2.054 CPU sec, 2.108 real sec...
sorting block 47, #reads = 1082499 mem = 536880392 peak = 1004859392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281884, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 337, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (353.51, 161.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676558 sequences (100000178 bp) in 2.185 CPU sec, 2.260 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283034, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 339, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (355.17, 162.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676560 reads in 144.237 CPU sec, 4.569 real sec
[M::process] read 678266 sequences (100000285 bp) in 1.948 CPU sec, 1.954 real sec...
[M::mem_process_seqs] Processed 676712 reads in 97.030 CPU sec, 3.052 real sec
sorting block 48, #reads = 1082851 mem = 536880514 peak = 1004859392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282449, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 341, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (357.61, 164.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679138 sequences (100000237 bp) in 2.874 CPU sec, 2.944 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283057, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 334, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (350.24, 162.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676558 reads in 146.645 CPU sec, 4.627 real sec
[M::process] read 678460 sequences (100000049 bp) in 2.044 CPU sec, 2.055 real sec...
[M::mem_process_seqs] Processed 678266 reads in 99.238 CPU sec, 3.113 real sec
sorting block 49, #reads = 1082948 mem = 536879848 peak = 1004859392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283459, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 329, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (346.31, 162.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678194 sequences (100000202 bp) in 2.490 CPU sec, 2.527 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283427, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 335, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (350.74, 163.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679138 reads in 146.294 CPU sec, 4.618 real sec
sorting block 50, #reads = 1082054 mem = 536880087 peak = 1004859392
[M::mem_process_seqs] Processed 678460 reads in 98.422 CPU sec, 3.082 real sec
[M::process] read 677846 sequences (100000244 bp) in 2.283 CPU sec, 2.285 real sec...
sorting block 51, #reads = 1083378 mem = 536878667 peak = 1007644672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282975, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 336, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (352.16, 163.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677468 sequences (100000257 bp) in 2.372 CPU sec, 2.442 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282810, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 339, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (354.76, 164.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677106 sequences (100000163 bp) in 1.640 CPU sec, 1.640 real sec...
[M::mem_process_seqs] Processed 678194 reads in 146.779 CPU sec, 4.647 real sec
[M::mem_process_seqs] Processed 677846 reads in 98.597 CPU sec, 3.113 real sec
sorting block 52, #reads = 1082702 mem = 536878857 peak = 1007644672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282420, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 342, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (356.09, 163.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676990 sequences (100000006 bp) in 2.276 CPU sec, 2.352 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282404, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 345, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (359.54, 165.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676614 sequences (100000124 bp) in 1.560 CPU sec, 1.554 real sec...
[M::mem_process_seqs] Processed 677468 reads in 142.574 CPU sec, 4.511 real sec
[M::mem_process_seqs] Processed 677106 reads in 94.463 CPU sec, 2.988 real sec
sorting block 53, #reads = 1082413 mem = 536877696 peak = 1007644672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281787, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 346, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (360.86, 165.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (232, 524, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1108)
[M::mem_pestat] mean and std.dev: (434.15, 134.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1400)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676518 sequences (100000017 bp) in 2.745 CPU sec, 2.786 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282860, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 350, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (363.71, 166.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676990 reads in 142.651 CPU sec, 4.491 real sec
[M::mem_process_seqs] Processed 676614 reads in 96.901 CPU sec, 3.047 real sec
[M::process] read 676330 sequences (100000228 bp) in 2.359 CPU sec, 2.371 real sec...
sorting block 54, #reads = 1082905 mem = 536881354 peak = 1007644672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282174, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 351, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (363.93, 165.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676308 sequences (100000153 bp) in 2.627 CPU sec, 2.682 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282701, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 353, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (366.34, 167.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676518 reads in 139.849 CPU sec, 4.422 real sec
sorting block 55, #reads = 1082102 mem = 536878931 peak = 1007644672
[M::mem_process_seqs] Processed 676330 reads in 92.695 CPU sec, 2.908 real sec
[M::process] read 676040 sequences (100000192 bp) in 2.438 CPU sec, 2.476 real sec...
sorting block 56, #reads = 1082376 mem = 536880210 peak = 1028022272
[M::process] read 675950 sequences (100000124 bp) in 2.577 CPU sec, 2.619 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282819, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 358, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (371.21, 169.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676040 reads in 79.288 CPU sec, 2.483 real sec
[M::process] read 677142 sequences (100000106 bp) in 1.917 CPU sec, 1.950 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282275, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 356, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (369.30, 168.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281977, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 359, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (372.03, 169.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676308 reads in 218.444 CPU sec, 6.908 real sec
sorting block 57, #reads = 1083924 mem = 536877208 peak = 1028022272
[M::mem_process_seqs] Processed 675950 reads in 109.129 CPU sec, 3.444 real sec
[M::process] read 677468 sequences (100000223 bp) in 1.759 CPU sec, 1.788 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282495, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 347, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (362.40, 167.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 58, #reads = 1082815 mem = 536881660 peak = 1029275648
[M::process] read 677300 sequences (100000124 bp) in 2.923 CPU sec, 3.011 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282373, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 341, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (358.20, 166.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677142 reads in 144.869 CPU sec, 4.596 real sec
[M::mem_process_seqs] Processed 677468 reads in 97.331 CPU sec, 3.060 real sec
[M::process] read 676860 sequences (100000109 bp) in 2.320 CPU sec, 2.380 real sec...
sorting block 59, #reads = 1083449 mem = 536878594 peak = 1029275648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283000, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 341, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (357.20, 164.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676810 sequences (100000250 bp) in 2.253 CPU sec, 2.300 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282322, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 345, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (359.74, 165.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677300 reads in 142.985 CPU sec, 4.516 real sec
[M::process] read 677010 sequences (100000111 bp) in 1.527 CPU sec, 1.528 real sec...
[M::mem_process_seqs] Processed 676860 reads in 95.970 CPU sec, 3.024 real sec
sorting block 60, #reads = 1084279 mem = 536878498 peak = 1029275648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282784, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 344, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (360.08, 165.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677110 sequences (100000229 bp) in 2.827 CPU sec, 2.885 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282610, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 342, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (358.35, 166.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676810 reads in 140.967 CPU sec, 4.447 real sec
[M::mem_process_seqs] Processed 677010 reads in 93.202 CPU sec, 2.932 real sec
[M::process] read 677054 sequences (100000135 bp) in 2.378 CPU sec, 2.412 real sec...
sorting block 61, #reads = 1082299 mem = 536882274 peak = 1029275648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283036, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 340, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (357.11, 164.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676888 sequences (100000127 bp) in 2.453 CPU sec, 2.508 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282281, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 340, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (357.33, 165.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677110 reads in 138.814 CPU sec, 4.397 real sec
sorting block 62, #reads = 1082714 mem = 536878724 peak = 1029275648
[M::mem_process_seqs] Processed 677054 reads in 90.193 CPU sec, 2.835 real sec
[M::process] read 677288 sequences (100000031 bp) in 1.937 CPU sec, 1.954 real sec...
sorting block 63, #reads = 1084204 mem = 536879688 peak = 1030361088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283225, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 339, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (356.51, 165.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677236 sequences (100000057 bp) in 2.679 CPU sec, 2.729 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282705, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 336, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (353.16, 163.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676888 reads in 139.538 CPU sec, 4.413 real sec
[M::mem_process_seqs] Processed 677288 reads in 93.705 CPU sec, 2.941 real sec
[M::process] read 677322 sequences (100000243 bp) in 2.347 CPU sec, 2.381 real sec...
sorting block 64, #reads = 1082068 mem = 536883421 peak = 1030361088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282263, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 336, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (353.65, 163.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678044 sequences (100000126 bp) in 2.242 CPU sec, 2.304 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282458, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 335, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (352.81, 163.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677236 reads in 143.135 CPU sec, 4.521 real sec
[M::mem_process_seqs] Processed 677322 reads in 95.170 CPU sec, 2.985 real sec
[M::process] read 677734 sequences (100000061 bp) in 2.221 CPU sec, 2.241 real sec...
sorting block 65, #reads = 1083651 mem = 536880578 peak = 1030361088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283090, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 331, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (348.58, 161.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677210 sequences (100000259 bp) in 2.185 CPU sec, 2.248 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282710, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 332, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (349.28, 161.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677070 sequences (100000048 bp) in 1.500 CPU sec, 1.498 real sec...
[M::mem_process_seqs] Processed 678044 reads in 145.933 CPU sec, 4.623 real sec
sorting block 66, #reads = 1082148 mem = 536880461 peak = 1030361088
[M::mem_process_seqs] Processed 677734 reads in 95.174 CPU sec, 3.010 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282749, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 335, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (353.09, 163.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677280 sequences (100000226 bp) in 2.792 CPU sec, 2.842 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282248, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 337, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (355.04, 165.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677210 reads in 142.998 CPU sec, 4.505 real sec
sorting block 67, #reads = 1081962 mem = 536877040 peak = 1030361088
[M::mem_process_seqs] Processed 677070 reads in 94.745 CPU sec, 2.970 real sec
[M::process] read 677216 sequences (100000044 bp) in 2.182 CPU sec, 2.200 real sec...
sorting block 68, #reads = 1082800 mem = 536878757 peak = 1062486016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282009, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 337, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (354.61, 165.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677304 sequences (100000292 bp) in 2.118 CPU sec, 2.177 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282600, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 337, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (355.01, 165.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677258 sequences (100000291 bp) in 1.590 CPU sec, 1.588 real sec...
[M::mem_process_seqs] Processed 677280 reads in 141.464 CPU sec, 4.480 real sec
[M::mem_process_seqs] Processed 677216 reads in 93.145 CPU sec, 2.942 real sec
sorting block 69, #reads = 1082662 mem = 536880647 peak = 1062486016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282762, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 336, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (353.86, 164.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676932 sequences (100000047 bp) in 2.754 CPU sec, 2.820 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282397, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 337, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (354.60, 164.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677304 reads in 136.730 CPU sec, 4.318 real sec
[M::process] read 676970 sequences (100000021 bp) in 2.443 CPU sec, 2.495 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282285, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 336, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (354.43, 163.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676932 reads in 76.203 CPU sec, 2.393 real sec
sorting block 70, #reads = 1082888 mem = 536883072 peak = 1062486016
[M::process] read 676696 sequences (100000153 bp) in 2.270 CPU sec, 2.274 real sec...
[M::mem_process_seqs] Processed 677258 reads in 199.252 CPU sec, 6.271 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282269, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 338, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (355.19, 164.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 71, #reads = 1081567 mem = 536878761 peak = 1095421952
[M::process] read 676848 sequences (100000018 bp) in 2.692 CPU sec, 2.758 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282359, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 338, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (355.72, 164.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676970 reads in 152.527 CPU sec, 4.811 real sec
[M::mem_process_seqs] Processed 676696 reads in 93.273 CPU sec, 2.932 real sec
sorting block 72, #reads = 1082133 mem = 536876657 peak = 1095421952
[M::process] read 677062 sequences (100000004 bp) in 2.683 CPU sec, 2.763 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282378, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 338, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (355.47, 164.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282159, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 676582 sequences (100000165 bp) in 2.259 CPU sec, 2.275 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (226, 339, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (356.91, 165.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676848 reads in 141.714 CPU sec, 4.479 real sec
[M::mem_process_seqs] Processed 677062 reads in 93.965 CPU sec, 2.944 real sec
sorting block 73, #reads = 1082552 mem = 536879286 peak = 1095421952
[M::process] read 676750 sequences (100000046 bp) in 2.654 CPU sec, 2.734 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282122, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 341, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (359.23, 166.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282538, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 340, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (356.68, 163.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678988 sequences (100000293 bp) in 2.378 CPU sec, 2.404 real sec...
[M::mem_process_seqs] Processed 676582 reads in 141.645 CPU sec, 4.486 real sec
[M::mem_process_seqs] Processed 676750 reads in 95.899 CPU sec, 3.013 real sec
sorting block 74, #reads = 1082479 mem = 536880910 peak = 1095421952
[M::process] read 678430 sequences (100000013 bp) in 2.590 CPU sec, 2.677 real sec...
sorting block 75, #reads = 1081642 mem = 536879524 peak = 1095421952
[M::process] read 678016 sequences (100000005 bp) in 2.220 CPU sec, 2.234 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282721, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 333, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (350.09, 163.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678430 reads in 81.775 CPU sec, 2.584 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282542, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 328, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (345.47, 161.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677692 sequences (100000135 bp) in 2.092 CPU sec, 2.203 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282324, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 337, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (354.50, 165.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678988 reads in 222.970 CPU sec, 7.062 real sec
[M::mem_process_seqs] Processed 678016 reads in 113.049 CPU sec, 3.566 real sec
sorting block 76, #reads = 1083436 mem = 536882426 peak = 1095421952
[M::process] read 677356 sequences (100000182 bp) in 2.409 CPU sec, 2.463 real sec...
[M::process] read 677194 sequences (100000239 bp) in 2.465 CPU sec, 2.502 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282477, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 344, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (359.66, 166.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677356 reads in 81.784 CPU sec, 2.565 real sec
sorting block 77, #reads = 1084294 mem = 536879519 peak = 1095421952
[M::process] read 676960 sequences (100000290 bp) in 1.999 CPU sec, 2.045 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282221, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 342, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (357.27, 166.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282001, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 346, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (362.14, 168.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677692 reads in 226.075 CPU sec, 7.134 real sec
[M::mem_process_seqs] Processed 677194 reads in 114.621 CPU sec, 3.595 real sec
[M::process] read 676788 sequences (100000149 bp) in 2.100 CPU sec, 2.111 real sec...
sorting block 78, #reads = 1084360 mem = 536879523 peak = 1095421952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282108, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 348, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (363.77, 168.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676910 sequences (100000096 bp) in 2.286 CPU sec, 2.364 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281830, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 350, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (364.97, 168.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676382 sequences (100000246 bp) in 1.500 CPU sec, 1.497 real sec...
[M::mem_process_seqs] Processed 676960 reads in 141.098 CPU sec, 4.470 real sec
sorting block 79, #reads = 1082818 mem = 536876920 peak = 1095421952
[M::mem_process_seqs] Processed 676788 reads in 94.739 CPU sec, 2.995 real sec
sorting block 80, #reads = 1083240 mem = 536880668 peak = 1095421952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282143, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 349, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (364.73, 168.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676102 sequences (100000261 bp) in 2.446 CPU sec, 2.510 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282085, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 352, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (366.50, 167.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676910 reads in 140.532 CPU sec, 4.437 real sec
[M::process] read 675898 sequences (100000202 bp) in 2.313 CPU sec, 2.322 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282019, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 354, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (368.15, 168.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676102 reads in 77.940 CPU sec, 2.451 real sec
[M::process] read 675706 sequences (100000193 bp) in 2.527 CPU sec, 2.554 real sec...
sorting block 81, #reads = 1083160 mem = 536877937 peak = 1095421952
[M::mem_process_seqs] Processed 676382 reads in 211.227 CPU sec, 6.659 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281386, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 356, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (369.56, 168.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675598 sequences (100000071 bp) in 1.927 CPU sec, 1.987 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282065, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 359, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (372.17, 169.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675898 reads in 151.504 CPU sec, 4.777 real sec
sorting block 82, #reads = 1082473 mem = 536880779 peak = 1095421952
[M::mem_process_seqs] Processed 675706 reads in 91.256 CPU sec, 2.866 real sec
[M::process] read 676754 sequences (100000155 bp) in 1.837 CPU sec, 1.873 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281833, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 361, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (373.80, 169.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 83, #reads = 1082779 mem = 536879690 peak = 1095421952
[M::process] read 677494 sequences (100000287 bp) in 2.130 CPU sec, 2.194 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282309, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 348, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (364.21, 168.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677058 sequences (100000193 bp) in 1.530 CPU sec, 1.536 real sec...
[M::mem_process_seqs] Processed 675598 reads in 139.464 CPU sec, 4.416 real sec
[M::mem_process_seqs] Processed 676754 reads in 93.368 CPU sec, 2.944 real sec
sorting block 84, #reads = 1083416 mem = 536884876 peak = 1095421952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282871, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 343, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (359.70, 167.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677050 sequences (100000190 bp) in 2.180 CPU sec, 2.250 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282020, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 345, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (361.12, 167.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676960 sequences (100000057 bp) in 1.560 CPU sec, 1.559 real sec...
[M::mem_process_seqs] Processed 677494 reads in 142.134 CPU sec, 4.495 real sec
[M::mem_process_seqs] Processed 677058 reads in 94.339 CPU sec, 2.985 real sec
sorting block 85, #reads = 1084162 mem = 536878737 peak = 1095421952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282333, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 346, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (362.23, 167.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677052 sequences (100000081 bp) in 3.163 CPU sec, 3.369 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282660, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 344, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (360.64, 166.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677050 reads in 146.842 CPU sec, 4.641 real sec
[M::mem_process_seqs] Processed 676960 reads in 97.284 CPU sec, 3.061 real sec
sorting block 86, #reads = 1083297 mem = 536878261 peak = 1095421952
[M::process] read 677004 sequences (100000013 bp) in 2.464 CPU sec, 2.502 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282319, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 340, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (357.02, 165.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677158 sequences (100000131 bp) in 2.148 CPU sec, 2.217 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282455, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 340, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (356.52, 164.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677052 reads in 148.562 CPU sec, 4.703 real sec
sorting block 87, #reads = 1083050 mem = 536879011 peak = 1095421952
[M::process] read 677338 sequences (100000022 bp) in 1.738 CPU sec, 1.740 real sec...
[M::mem_process_seqs] Processed 677004 reads in 97.651 CPU sec, 3.071 real sec
sorting block 88, #reads = 1083770 mem = 536878639 peak = 1118617600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282253, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 338, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (354.68, 163.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677330 sequences (100000093 bp) in 2.873 CPU sec, 2.946 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282201, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 337, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (354.16, 163.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677158 reads in 147.816 CPU sec, 4.664 real sec
[M::mem_process_seqs] Processed 677338 reads in 97.635 CPU sec, 3.063 real sec
[M::process] read 676950 sequences (100000239 bp) in 2.062 CPU sec, 2.071 real sec...
sorting block 89, #reads = 1083104 mem = 536880622 peak = 1118617600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282118, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 336, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (353.39, 163.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676986 sequences (100000066 bp) in 1.966 CPU sec, 2.024 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282127, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 337, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (353.37, 162.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676990 sequences (100000057 bp) in 1.570 CPU sec, 1.572 real sec...
[M::mem_process_seqs] Processed 677330 reads in 144.828 CPU sec, 4.594 real sec
[M::mem_process_seqs] Processed 676950 reads in 95.800 CPU sec, 3.030 real sec
sorting block 90, #reads = 1082637 mem = 536880647 peak = 1118617600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282323, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 336, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (353.21, 162.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676840 sequences (100000018 bp) in 2.309 CPU sec, 2.370 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282294, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 338, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (353.83, 162.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676908 sequences (100000237 bp) in 1.570 CPU sec, 1.578 real sec...
[M::mem_process_seqs] Processed 676986 reads in 145.760 CPU sec, 4.612 real sec
sorting block 91, #reads = 1081653 mem = 536880771 peak = 1118617600
[M::mem_process_seqs] Processed 676990 reads in 95.173 CPU sec, 3.006 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282465, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 338, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (354.82, 162.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677172 sequences (100000005 bp) in 2.171 CPU sec, 2.235 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282382, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 337, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (354.96, 163.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677026 sequences (100000196 bp) in 1.800 CPU sec, 1.797 real sec...
[M::mem_process_seqs] Processed 676840 reads in 142.032 CPU sec, 4.482 real sec
sorting block 92, #reads = 1082610 mem = 536880115 peak = 1118617600
[M::mem_process_seqs] Processed 676908 reads in 94.119 CPU sec, 2.964 real sec
sorting block 93, #reads = 1082752 mem = 536879369 peak = 1118617600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282700, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 337, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (355.32, 164.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676978 sequences (100000209 bp) in 2.803 CPU sec, 2.840 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282232, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 338, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (355.80, 164.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677172 reads in 147.548 CPU sec, 4.658 real sec
[M::mem_process_seqs] Processed 677026 reads in 98.622 CPU sec, 3.094 real sec
[M::process] read 676990 sequences (100000006 bp) in 2.350 CPU sec, 2.372 real sec...
sorting block 94, #reads = 1083266 mem = 536879816 peak = 1118617600
[M::process] read 676872 sequences (100000072 bp) in 2.508 CPU sec, 2.574 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282425, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 335, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (352.06, 161.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676990 reads in 82.720 CPU sec, 2.591 real sec
[M::process] read 676792 sequences (100000248 bp) in 2.365 CPU sec, 2.387 real sec...
sorting block 95, #reads = 1082021 mem = 536879588 peak = 1118617600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282318, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 338, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (355.56, 164.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282007, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 337, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (354.53, 163.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676978 reads in 228.327 CPU sec, 7.211 real sec
[M::mem_process_seqs] Processed 676872 reads in 113.852 CPU sec, 3.579 real sec
[M::process] read 676806 sequences (100000221 bp) in 2.602 CPU sec, 2.645 real sec...
sorting block 96, #reads = 1081858 mem = 536880991 peak = 1118617600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281879, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 339, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (355.82, 162.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676472 sequences (100000080 bp) in 2.636 CPU sec, 2.694 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282267, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 339, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (355.23, 162.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676792 reads in 143.004 CPU sec, 4.536 real sec
[M::mem_process_seqs] Processed 676806 reads in 95.586 CPU sec, 3.005 real sec
[M::process] read 676360 sequences (100000063 bp) in 2.481 CPU sec, 2.531 real sec...
sorting block 97, #reads = 1082094 mem = 536880262 peak = 1118617600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282256, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 341, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (357.13, 163.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676534 sequences (100000047 bp) in 2.535 CPU sec, 2.577 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282414, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 342, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (357.93, 163.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676472 reads in 141.635 CPU sec, 4.486 real sec
[M::mem_process_seqs] Processed 676360 reads in 94.844 CPU sec, 2.976 real sec
sorting block 98, #reads = 1082629 mem = 536876725 peak = 1118617600
[M::process] read 676310 sequences (100000045 bp) in 2.557 CPU sec, 2.597 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282582, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 341, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (357.78, 164.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676818 sequences (100000276 bp) in 2.116 CPU sec, 2.148 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282470, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 344, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (359.93, 164.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676534 reads in 138.870 CPU sec, 4.399 real sec
sorting block 99, #reads = 1082090 mem = 536881746 peak = 1118617600
[M::mem_process_seqs] Processed 676310 reads in 92.264 CPU sec, 2.900 real sec
[M::process] read 682342 sequences (100000193 bp) in 2.147 CPU sec, 2.173 real sec...
sorting block 100, #reads = 1082815 mem = 536878989 peak = 1118617600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282370, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 341, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (358.54, 165.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681604 sequences (100000200 bp) in 2.663 CPU sec, 2.700 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284668, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 308, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (331.41, 161.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676818 reads in 149.657 CPU sec, 4.736 real sec
[M::mem_process_seqs] Processed 682342 reads in 102.383 CPU sec, 3.216 real sec
[M::process] read 681030 sequences (100000065 bp) in 2.429 CPU sec, 2.458 real sec...
sorting block 101, #reads = 1081422 mem = 536879799 peak = 1118617600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283818, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 315, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (337.44, 163.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680716 sequences (100000123 bp) in 2.611 CPU sec, 2.655 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283948, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 319, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (340.51, 164.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681604 reads in 155.089 CPU sec, 4.897 real sec
[M::mem_process_seqs] Processed 681030 reads in 102.161 CPU sec, 3.206 real sec
[M::process] read 680744 sequences (100000248 bp) in 2.251 CPU sec, 2.267 real sec...
sorting block 102, #reads = 1082650 mem = 536878061 peak = 1118617600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283898, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 321, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (341.03, 162.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680206 sequences (100000184 bp) in 2.705 CPU sec, 2.756 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283990, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 321, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (339.23, 161.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680716 reads in 160.999 CPU sec, 5.084 real sec
[M::process] read 679836 sequences (100000278 bp) in 2.284 CPU sec, 2.289 real sec...
[M::mem_process_seqs] Processed 680744 reads in 106.806 CPU sec, 3.346 real sec
sorting block 103, #reads = 1082002 mem = 536881012 peak = 1118617600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283474, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 326, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (342.55, 160.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679148 sequences (100000057 bp) in 2.697 CPU sec, 2.752 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283577, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 329, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (345.32, 162.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680206 reads in 160.145 CPU sec, 5.048 real sec
[M::process] read 678690 sequences (100000133 bp) in 2.090 CPU sec, 2.093 real sec...
sorting block 104, #reads = 1080343 mem = 536878258 peak = 1118617600
[M::mem_process_seqs] Processed 679836 reads in 105.216 CPU sec, 3.311 real sec
sorting block 105, #reads = 1079775 mem = 536877385 peak = 1118617600
[M::process] read 678778 sequences (100000229 bp) in 2.650 CPU sec, 2.696 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282670, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 335, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (350.20, 162.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678690 reads in 87.593 CPU sec, 2.737 real sec
[M::process] read 678748 sequences (100000179 bp) in 2.306 CPU sec, 2.319 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282797, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 333, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (348.16, 161.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283402, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 336, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (351.54, 163.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679148 reads in 241.723 CPU sec, 7.621 real sec
sorting block 106, #reads = 1079532 mem = 536879888 peak = 1118617600
[M::process] read 680218 sequences (100000066 bp) in 2.248 CPU sec, 2.264 real sec...
[M::mem_process_seqs] Processed 678778 reads in 120.633 CPU sec, 3.803 real sec
sorting block 107, #reads = 1080510 mem = 536880550 peak = 1118617600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282466, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 335, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (349.28, 161.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680618 sequences (100000065 bp) in 2.877 CPU sec, 2.958 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283480, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 321, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (338.64, 158.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678748 reads in 158.633 CPU sec, 5.003 real sec
[M::process] read 680792 sequences (100000127 bp) in 2.147 CPU sec, 2.161 real sec...
[M::mem_process_seqs] Processed 680218 reads in 105.733 CPU sec, 3.320 real sec
sorting block 108, #reads = 1080093 mem = 536877717 peak = 1118617600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283718, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 318, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (336.99, 159.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680438 sequences (100000050 bp) in 2.640 CPU sec, 2.702 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284446, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 317, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (335.83, 158.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680618 reads in 160.016 CPU sec, 5.050 real sec
[M::process] read 680736 sequences (100000205 bp) in 2.218 CPU sec, 2.225 real sec...
[M::mem_process_seqs] Processed 680792 reads in 105.644 CPU sec, 3.316 real sec
sorting block 109, #reads = 1080445 mem = 536877092 peak = 1118617600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284641, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 319, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (337.46, 158.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680784 sequences (100000241 bp) in 2.663 CPU sec, 2.715 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284193, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 318, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (336.91, 158.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680438 reads in 160.245 CPU sec, 5.054 real sec
[M::process] read 679982 sequences (100000038 bp) in 2.298 CPU sec, 2.299 real sec...
[M::mem_process_seqs] Processed 680736 reads in 106.594 CPU sec, 3.349 real sec
sorting block 110, #reads = 1080886 mem = 536879397 peak = 1118617600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284170, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 319, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (337.53, 158.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679078 sequences (100000037 bp) in 2.785 CPU sec, 2.819 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283368, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 325, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (341.89, 159.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678992 sequences (100000058 bp) in 2.210 CPU sec, 2.215 real sec...
[M::mem_process_seqs] Processed 680784 reads in 169.280 CPU sec, 5.336 real sec
sorting block 111, #reads = 1077243 mem = 536878004 peak = 1118617600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283290, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 330, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (346.76, 161.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678714 sequences (100000029 bp) in 2.542 CPU sec, 2.551 real sec...
[M::mem_process_seqs] Processed 679078 reads in 90.762 CPU sec, 2.848 real sec
sorting block 112, #reads = 1078436 mem = 536878255 peak = 1118617600
[M::mem_process_seqs] Processed 679982 reads in 220.798 CPU sec, 6.965 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282657, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 325, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (341.87, 158.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682172 sequences (100000196 bp) in 2.986 CPU sec, 3.053 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283330, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 332, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (347.83, 160.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678992 reads in 180.040 CPU sec, 5.679 real sec
sorting block 113, #reads = 1078133 mem = 536879967 peak = 1118617600
[M::mem_process_seqs] Processed 678714 reads in 109.576 CPU sec, 3.437 real sec
[M::process] read 679942 sequences (100000082 bp) in 2.643 CPU sec, 2.675 real sec...
sorting block 114, #reads = 1078816 mem = 536877138 peak = 1118617600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284387, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 306, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (322.99, 149.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679752 sequences (100000264 bp) in 2.684 CPU sec, 2.744 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 283783, 2, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (260, 333, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (355.15, 120.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 319, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (336.43, 155.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 679260 sequences (100000174 bp) in 2.230 CPU sec, 2.227 real sec...
[M::mem_process_seqs] Processed 682172 reads in 189.984 CPU sec, 5.997 real sec
[M::mem_process_seqs] Processed 679942 reads in 123.328 CPU sec, 3.885 real sec
sorting block 115, #reads = 1071711 mem = 536878650 peak = 1118617600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283813, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 321, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (336.81, 154.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678508 sequences (100000237 bp) in 2.900 CPU sec, 2.956 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283612, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 323, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (339.38, 155.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678726 sequences (100000169 bp) in 2.259 CPU sec, 2.257 real sec...
[M::mem_process_seqs] Processed 679752 reads in 179.326 CPU sec, 5.654 real sec
[M::mem_process_seqs] Processed 679260 reads in 120.363 CPU sec, 3.797 real sec
sorting block 116, #reads = 1074356 mem = 536879710 peak = 1118617600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282884, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 328, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (344.62, 158.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678510 sequences (100000142 bp) in 2.325 CPU sec, 2.406 real sec...
[M::process] read 678284 sequences (100000283 bp) in 1.680 CPU sec, 1.680 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283537, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 327, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (344.05, 158.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678508 reads in 173.667 CPU sec, 5.502 real sec
[M::mem_process_seqs] Processed 678726 reads in 113.522 CPU sec, 3.614 real sec
sorting block 117, #reads = 1075566 mem = 536880474 peak = 1118617600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283598, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 331, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (347.44, 160.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678432 sequences (100000072 bp) in 2.884 CPU sec, 2.927 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283531, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 332, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (348.79, 160.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678510 reads in 170.738 CPU sec, 5.386 real sec
[M::process] read 678880 sequences (100000014 bp) in 2.260 CPU sec, 2.262 real sec...
sorting block 118, #reads = 1076279 mem = 536880209 peak = 1118617600
[M::mem_process_seqs] Processed 678284 reads in 112.083 CPU sec, 3.529 real sec
sorting block 119, #reads = 1076205 mem = 536878468 peak = 1118617600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283556, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 329, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (347.11, 160.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678438 sequences (100000292 bp) in 2.714 CPU sec, 2.781 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283464, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 325, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (343.46, 160.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678432 reads in 173.199 CPU sec, 5.469 real sec
[M::process] read 680084 sequences (100000137 bp) in 2.378 CPU sec, 2.374 real sec...
[M::mem_process_seqs] Processed 678880 reads in 114.493 CPU sec, 3.598 real sec
sorting block 120, #reads = 1076176 mem = 536879660 peak = 1118617600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283554, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 329, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (348.71, 163.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686222 sequences (100000191 bp) in 2.936 CPU sec, 2.982 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283816, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 321, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (341.39, 163.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678438 reads in 170.907 CPU sec, 5.388 real sec
[M::process] read 683320 sequences (100000285 bp) in 2.308 CPU sec, 2.306 real sec...
[M::mem_process_seqs] Processed 680084 reads in 112.395 CPU sec, 3.539 real sec
sorting block 121, #reads = 1075832 mem = 536878858 peak = 1118617600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286210, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 285, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868)
[M::mem_pestat] mean and std.dev: (309.45, 150.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1095)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682136 sequences (100000099 bp) in 2.328 CPU sec, 2.379 real sec...
[M::process] read 681900 sequences (100000091 bp) in 1.710 CPU sec, 1.705 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285373, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 301, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (322.11, 154.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686222 reads in 177.674 CPU sec, 5.643 real sec
sorting block 122, #reads = 1076141 mem = 536879092 peak = 1118617600
[M::mem_process_seqs] Processed 683320 reads in 115.300 CPU sec, 3.664 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285041, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 309, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (329.28, 156.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681688 sequences (100000201 bp) in 2.393 CPU sec, 2.451 real sec...
[M::process] read 680556 sequences (100000131 bp) in 1.720 CPU sec, 1.720 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285002, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 311, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (330.33, 156.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682136 reads in 169.370 CPU sec, 5.369 real sec
sorting block 123, #reads = 1075402 mem = 536878643 peak = 1118617600
[M::mem_process_seqs] Processed 681900 reads in 112.331 CPU sec, 3.565 real sec
sorting block 124, #reads = 1077862 mem = 536876490 peak = 1118617600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284047, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 313, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (332.21, 157.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680034 sequences (100000015 bp) in 2.698 CPU sec, 2.744 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284107, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 320, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (336.53, 156.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681688 reads in 168.253 CPU sec, 5.302 real sec
[M::process] read 679656 sequences (100000240 bp) in 2.260 CPU sec, 2.264 real sec...
[M::mem_process_seqs] Processed 680556 reads in 109.849 CPU sec, 3.457 real sec
sorting block 125, #reads = 1075434 mem = 536877592 peak = 1118617600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284313, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 324, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (340.02, 157.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679278 sequences (100000277 bp) in 2.902 CPU sec, 2.951 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283570, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 327, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (342.36, 157.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680034 reads in 164.880 CPU sec, 5.192 real sec
[M::process] read 680100 sequences (100000044 bp) in 2.339 CPU sec, 2.333 real sec...
[M::mem_process_seqs] Processed 679656 reads in 111.980 CPU sec, 3.521 real sec
sorting block 126, #reads = 1076661 mem = 536877261 peak = 1118617600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284058, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 329, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (344.07, 159.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680330 sequences (100000144 bp) in 2.738 CPU sec, 2.795 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284666, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 321, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (339.64, 159.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679278 reads in 171.653 CPU sec, 5.419 real sec
[M::process] read 680136 sequences (100000271 bp) in 2.279 CPU sec, 2.284 real sec...
sorting block 127, #reads = 1076659 mem = 536879058 peak = 1118617600
[M::mem_process_seqs] Processed 680100 reads in 114.868 CPU sec, 3.616 real sec
sorting block 128, #reads = 1079666 mem = 536878034 peak = 1118617600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283919, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 319, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (337.84, 159.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680018 sequences (100000026 bp) in 2.933 CPU sec, 2.984 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283835, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 319, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (337.98, 159.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679596 sequences (100000159 bp) in 2.300 CPU sec, 2.296 real sec...
[M::mem_process_seqs] Processed 680330 reads in 176.910 CPU sec, 5.577 real sec
[M::mem_process_seqs] Processed 680136 reads in 115.820 CPU sec, 3.651 real sec
sorting block 129, #reads = 1079908 mem = 536879748 peak = 1118617600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283404, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 320, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (338.97, 159.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679694 sequences (100000181 bp) in 2.515 CPU sec, 2.584 real sec...
[M::process] read 680136 sequences (100000052 bp) in 1.730 CPU sec, 1.731 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283649, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 322, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (340.29, 159.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680018 reads in 177.345 CPU sec, 5.632 real sec
[M::mem_process_seqs] Processed 679596 reads in 116.873 CPU sec, 3.701 real sec
sorting block 130, #reads = 1080886 mem = 536882108 peak = 1118617600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283863, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 325, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (342.28, 160.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679892 sequences (100000257 bp) in 2.550 CPU sec, 2.619 real sec...
[M::process] read 679796 sequences (100000125 bp) in 1.820 CPU sec, 1.811 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284131, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 321, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (339.32, 159.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679694 reads in 181.045 CPU sec, 5.739 real sec
[M::mem_process_seqs] Processed 680136 reads in 119.947 CPU sec, 3.801 real sec
sorting block 131, #reads = 1079239 mem = 536881821 peak = 1118617600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283570, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 322, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (339.46, 158.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680520 sequences (100000057 bp) in 2.866 CPU sec, 2.939 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283508, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 324, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (342.10, 160.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680966 sequences (100000182 bp) in 2.370 CPU sec, 2.366 real sec...
[M::mem_process_seqs] Processed 679892 reads in 187.735 CPU sec, 5.933 real sec
sorting block 132, #reads = 1077864 mem = 536879630 peak = 1118617600
[M::mem_process_seqs] Processed 679796 reads in 123.472 CPU sec, 3.888 real sec
sorting block 133, #reads = 1078415 mem = 536885056 peak = 1118617600
[M::process] read 678218 sequences (100000001 bp) in 2.875 CPU sec, 2.954 real sec...
[M::process] read 677862 sequences (100000281 bp) in 2.321 CPU sec, 2.329 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284566, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 314, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (333.84, 157.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680966 reads in 118.046 CPU sec, 3.726 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284465, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 318, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (337.05, 158.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283031, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 334, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (352.76, 165.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680520 reads in 305.260 CPU sec, 9.655 real sec
[M::process] read 677996 sequences (100000119 bp) in 2.525 CPU sec, 2.531 real sec...
sorting block 134, #reads = 1074361 mem = 536879095 peak = 1118617600
[M::mem_process_seqs] Processed 678218 reads in 147.083 CPU sec, 4.681 real sec
[M::process] read 677902 sequences (100000064 bp) in 2.692 CPU sec, 2.791 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283383, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 335, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (353.56, 166.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677680 sequences (100000279 bp) in 2.190 CPU sec, 2.188 real sec...
[M::mem_process_seqs] Processed 677996 reads in 103.526 CPU sec, 3.261 real sec
sorting block 135, #reads = 1076887 mem = 536877817 peak = 1118617600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283089, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 334, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (353.63, 166.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283009, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 336, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (355.51, 168.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677862 reads in 278.402 CPU sec, 8.806 real sec
[M::process] read 677442 sequences (100000237 bp) in 2.592 CPU sec, 2.609 real sec...
[M::mem_process_seqs] Processed 677902 reads in 142.946 CPU sec, 4.534 real sec
sorting block 136, #reads = 1080362 mem = 536879413 peak = 1119793152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282772, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 340, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (358.59, 169.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677344 sequences (100000226 bp) in 2.426 CPU sec, 2.524 real sec...
[M::process] read 677382 sequences (100000292 bp) in 1.944 CPU sec, 1.940 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283357, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 343, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (361.67, 171.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677680 reads in 179.214 CPU sec, 5.678 real sec
sorting block 137, #reads = 1079333 mem = 536881733 peak = 1119793152
[M::mem_process_seqs] Processed 677442 reads in 118.522 CPU sec, 3.759 real sec
sorting block 138, #reads = 1079813 mem = 536878380 peak = 1119793152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282705, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 344, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (362.99, 172.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677352 sequences (100000011 bp) in 2.743 CPU sec, 2.823 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282616, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 342, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (362.44, 172.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677274 sequences (100000148 bp) in 2.270 CPU sec, 2.268 real sec...
[M::mem_process_seqs] Processed 677344 reads in 168.738 CPU sec, 5.315 real sec
[M::mem_process_seqs] Processed 677382 reads in 113.594 CPU sec, 3.579 real sec
sorting block 139, #reads = 1079617 mem = 536879527 peak = 1119793152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282423, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 342, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (361.84, 172.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677716 sequences (100000079 bp) in 3.091 CPU sec, 3.144 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282985, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 342, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (362.63, 173.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677352 reads in 176.705 CPU sec, 5.568 real sec
[M::process] read 681590 sequences (100000167 bp) in 2.379 CPU sec, 2.381 real sec...
[M::mem_process_seqs] Processed 677274 reads in 120.144 CPU sec, 3.774 real sec
sorting block 140, #reads = 1078673 mem = 536881413 peak = 1119793152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282104, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 338, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (359.16, 171.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680464 sequences (100000076 bp) in 3.034 CPU sec, 3.102 real sec...
[M::process] read 679898 sequences (100000071 bp) in 2.270 CPU sec, 2.270 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283908, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 311, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (336.46, 165.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677716 reads in 202.881 CPU sec, 6.410 real sec
sorting block 141, #reads = 1076220 mem = 536876581 peak = 1119793152
[M::mem_process_seqs] Processed 681590 reads in 134.371 CPU sec, 4.264 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283223, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 325, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (346.79, 169.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679404 sequences (100000105 bp) in 2.791 CPU sec, 2.875 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283384, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 679444 sequences (100000273 bp) in 1.770 CPU sec, 1.767 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (212, 330, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (350.97, 170.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680464 reads in 168.689 CPU sec, 5.334 real sec
sorting block 142, #reads = 1072333 mem = 536881022 peak = 1119793152
[M::mem_process_seqs] Processed 679898 reads in 109.323 CPU sec, 3.461 real sec
sorting block 143, #reads = 1082936 mem = 536880497 peak = 1119793152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 283142, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 332, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (353.28, 170.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679274 sequences (100000104 bp) in 3.070 CPU sec, 3.138 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283493, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 331, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (351.12, 168.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679404 reads in 165.467 CPU sec, 5.217 real sec
[M::process] read 678134 sequences (100000195 bp) in 2.392 CPU sec, 2.402 real sec...
[M::mem_process_seqs] Processed 679444 reads in 111.030 CPU sec, 3.483 real sec
sorting block 144, #reads = 1082817 mem = 536879185 peak = 1119793152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283348, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 332, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (351.21, 167.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677986 sequences (100000218 bp) in 2.780 CPU sec, 2.843 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282515, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 339, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (355.53, 167.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679274 reads in 166.607 CPU sec, 5.277 real sec
[M::mem_process_seqs] Processed 678134 reads in 109.894 CPU sec, 3.445 real sec
[M::process] read 677920 sequences (100000128 bp) in 2.432 CPU sec, 2.448 real sec...
sorting block 145, #reads = 1082214 mem = 536879951 peak = 1119793152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282644, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 341, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (357.30, 167.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677682 sequences (100000101 bp) in 2.844 CPU sec, 2.901 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282487, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 344, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (359.66, 168.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677986 reads in 162.131 CPU sec, 5.130 real sec
[M::mem_process_seqs] Processed 677920 reads in 107.114 CPU sec, 3.357 real sec
[M::process] read 677648 sequences (100000042 bp) in 2.301 CPU sec, 2.319 real sec...
sorting block 146, #reads = 1082677 mem = 536882139 peak = 1119793152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283626, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 346, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (360.82, 168.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678022 sequences (100000239 bp) in 2.758 CPU sec, 2.824 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282603, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 347, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (362.84, 170.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677682 reads in 162.904 CPU sec, 5.160 real sec
sorting block 147, #reads = 1081316 mem = 536878033 peak = 1119793152
[M::mem_process_seqs] Processed 677648 reads in 109.289 CPU sec, 3.429 real sec
[M::process] read 679168 sequences (100000215 bp) in 2.495 CPU sec, 2.535 real sec...
sorting block 148, #reads = 1081784 mem = 536878501 peak = 1119793152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283481, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 344, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (360.45, 169.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680222 sequences (100000045 bp) in 2.875 CPU sec, 2.948 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283141, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 331, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (350.39, 167.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678022 reads in 163.783 CPU sec, 5.186 real sec
[M::mem_process_seqs] Processed 679168 reads in 110.671 CPU sec, 3.473 real sec
[M::process] read 679060 sequences (100000053 bp) in 2.650 CPU sec, 2.681 real sec...
sorting block 149, #reads = 1081905 mem = 536877246 peak = 1119793152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283917, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 319, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (341.75, 165.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678778 sequences (100000032 bp) in 2.881 CPU sec, 2.949 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283633, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 331, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (350.62, 167.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680222 reads in 164.482 CPU sec, 5.211 real sec
[M::mem_process_seqs] Processed 679060 reads in 107.874 CPU sec, 3.390 real sec
[M::process] read 678476 sequences (100000294 bp) in 2.572 CPU sec, 2.600 real sec...
sorting block 150, #reads = 1083462 mem = 536877979 peak = 1119793152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283145, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 335, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (354.38, 169.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678578 sequences (100000229 bp) in 2.921 CPU sec, 3.003 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283220, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 336, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (355.03, 169.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678778 reads in 167.001 CPU sec, 5.286 real sec
[M::mem_process_seqs] Processed 678476 reads in 114.036 CPU sec, 3.577 real sec
sorting block 151, #reads = 1081765 mem = 536879310 peak = 1119793152
[M::process] read 677974 sequences (100000066 bp) in 2.286 CPU sec, 2.313 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283688, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 337, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (355.48, 168.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 152, #reads = 1080814 mem = 536881543 peak = 1119793152
[M::process] read 677606 sequences (100000059 bp) in 2.899 CPU sec, 2.978 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283795, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 341, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (359.47, 170.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678578 reads in 169.917 CPU sec, 5.370 real sec
[M::process] read 677282 sequences (100000217 bp) in 2.117 CPU sec, 2.130 real sec...
[M::mem_process_seqs] Processed 677974 reads in 110.710 CPU sec, 3.476 real sec
sorting block 153, #reads = 1081156 mem = 536879697 peak = 1119793152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282583, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 342, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (360.55, 170.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677444 sequences (100000055 bp) in 2.987 CPU sec, 3.067 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282922, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 344, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (363.07, 172.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678236 sequences (100000298 bp) in 1.820 CPU sec, 1.823 real sec...
[M::mem_process_seqs] Processed 677606 reads in 167.952 CPU sec, 5.300 real sec
[M::mem_process_seqs] Processed 677282 reads in 112.505 CPU sec, 3.545 real sec
sorting block 154, #reads = 1081122 mem = 536880843 peak = 1119793152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283048, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 342, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (362.52, 172.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678576 sequences (100000247 bp) in 2.645 CPU sec, 2.704 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283339, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 335, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (355.57, 169.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677444 reads in 167.949 CPU sec, 5.298 real sec
[M::process] read 677866 sequences (100000192 bp) in 2.309 CPU sec, 2.315 real sec...
sorting block 155, #reads = 1081995 mem = 536879239 peak = 1119793152
[M::mem_process_seqs] Processed 678236 reads in 115.895 CPU sec, 3.645 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283383, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 331, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (351.53, 167.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677974 sequences (100000042 bp) in 2.917 CPU sec, 2.986 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283157, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 677874 sequences (100000171 bp) in 1.900 CPU sec, 1.905 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (219, 336, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (357.51, 171.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678576 reads in 178.114 CPU sec, 5.623 real sec
sorting block 156, #reads = 1079766 mem = 536879585 peak = 1119793152
[M::mem_process_seqs] Processed 677866 reads in 117.856 CPU sec, 3.727 real sec
sorting block 157, #reads = 1077686 mem = 536881968 peak = 1119793152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283159, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 337, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (358.51, 172.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677798 sequences (100000193 bp) in 2.845 CPU sec, 2.935 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282820, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 339, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (359.71, 171.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677974 reads in 173.202 CPU sec, 5.462 real sec
[M::process] read 678164 sequences (100000218 bp) in 2.428 CPU sec, 2.422 real sec...
[M::mem_process_seqs] Processed 677874 reads in 115.686 CPU sec, 3.631 real sec
sorting block 158, #reads = 1079701 mem = 536878762 peak = 1119793152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282446, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 337, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (358.55, 171.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677992 sequences (100000165 bp) in 2.765 CPU sec, 2.848 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283179, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 334, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (356.90, 172.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677982 sequences (100000023 bp) in 2.340 CPU sec, 2.338 real sec...
[M::mem_process_seqs] Processed 677798 reads in 178.773 CPU sec, 5.642 real sec
[M::mem_process_seqs] Processed 678164 reads in 119.253 CPU sec, 3.753 real sec
sorting block 159, #reads = 1080972 mem = 536878447 peak = 1119793152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282922, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 336, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (359.90, 174.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678206 sequences (100000270 bp) in 2.834 CPU sec, 2.889 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282891, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 337, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (360.30, 175.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678614 sequences (100000250 bp) in 2.020 CPU sec, 2.020 real sec...
[M::mem_process_seqs] Processed 677992 reads in 176.565 CPU sec, 5.580 real sec
sorting block 160, #reads = 1079308 mem = 536878126 peak = 1119793152
[M::mem_process_seqs] Processed 677982 reads in 120.050 CPU sec, 3.791 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282857, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 332, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (356.59, 173.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678166 sequences (100000038 bp) in 3.083 CPU sec, 3.136 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283992, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 327, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (352.05, 171.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681638 sequences (100000299 bp) in 2.420 CPU sec, 2.414 real sec...
[M::mem_process_seqs] Processed 678206 reads in 183.502 CPU sec, 5.791 real sec
sorting block 161, #reads = 1080024 mem = 536882512 peak = 1119793152
[M::process] read 683168 sequences (100000175 bp) in 1.981 CPU sec, 1.988 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282292, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 331, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (354.31, 170.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678166 reads in 114.109 CPU sec, 3.827 real sec
sorting block 162, #reads = 1074817 mem = 536876984 peak = 1119793152
[M::mem_process_seqs] Processed 678614 reads in 262.305 CPU sec, 8.521 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283941, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 311, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (338.25, 168.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681534 sequences (100000208 bp) in 2.998 CPU sec, 3.103 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284937, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 305, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (331.60, 164.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681638 reads in 214.406 CPU sec, 6.793 real sec
[M::process] read 680486 sequences (100000023 bp) in 2.476 CPU sec, 2.493 real sec...
[M::mem_process_seqs] Processed 683168 reads in 125.269 CPU sec, 3.934 real sec
sorting block 163, #reads = 1073637 mem = 536879155 peak = 1119793152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284372, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 317, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (340.13, 166.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 164, #reads = 1078365 mem = 536878739 peak = 1119793152
[M::process] read 679900 sequences (100000194 bp) in 3.054 CPU sec, 3.132 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283853, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 324, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (344.99, 166.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681534 reads in 182.913 CPU sec, 5.780 real sec
[M::process] read 679182 sequences (100000101 bp) in 2.360 CPU sec, 2.366 real sec...
[M::mem_process_seqs] Processed 680486 reads in 120.357 CPU sec, 3.790 real sec
sorting block 165, #reads = 1078937 mem = 536878035 peak = 1119793152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283550, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 329, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (348.78, 167.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679448 sequences (100000052 bp) in 3.092 CPU sec, 3.222 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283480, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 334, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (351.88, 166.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678394 sequences (100000146 bp) in 2.357 CPU sec, 2.364 real sec...
[M::mem_process_seqs] Processed 679900 reads in 193.267 CPU sec, 6.126 real sec
[M::mem_process_seqs] Processed 679182 reads in 128.030 CPU sec, 4.052 real sec
sorting block 166, #reads = 1078682 mem = 536882726 peak = 1119793152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283683, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 330, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (349.55, 166.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678298 sequences (100000013 bp) in 2.916 CPU sec, 3.075 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283132, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 341, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (356.38, 166.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679448 reads in 190.244 CPU sec, 6.059 real sec
[M::process] read 677754 sequences (100000098 bp) in 2.351 CPU sec, 2.356 real sec...
[M::mem_process_seqs] Processed 678394 reads in 127.621 CPU sec, 4.039 real sec
sorting block 167, #reads = 1079009 mem = 536878132 peak = 1119793152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283210, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 342, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (356.82, 165.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677432 sequences (100000284 bp) in 3.125 CPU sec, 3.301 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282677, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 344, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (359.38, 167.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678298 reads in 187.235 CPU sec, 5.980 real sec
[M::process] read 678592 sequences (100000110 bp) in 2.386 CPU sec, 2.414 real sec...
sorting block 168, #reads = 1078365 mem = 536876239 peak = 1119793152
[M::mem_process_seqs] Processed 677754 reads in 125.263 CPU sec, 3.967 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283450, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 350, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (364.10, 168.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 169, #reads = 1077673 mem = 536881940 peak = 1145675776
[M::process] read 678974 sequences (100000076 bp) in 2.997 CPU sec, 3.204 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283447, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 335, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (352.46, 165.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677432 reads in 186.096 CPU sec, 5.954 real sec
[M::mem_process_seqs] Processed 678592 reads in 126.132 CPU sec, 3.982 real sec
[M::process] read 678260 sequences (100000197 bp) in 2.563 CPU sec, 2.603 real sec...
sorting block 170, #reads = 1079333 mem = 536879556 peak = 1145675776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283875, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 332, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (350.35, 164.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677808 sequences (100000297 bp) in 2.865 CPU sec, 3.038 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283564, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 336, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (353.00, 164.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678974 reads in 185.771 CPU sec, 5.949 real sec
[M::process] read 677576 sequences (100000268 bp) in 2.342 CPU sec, 2.367 real sec...
[M::mem_process_seqs] Processed 678260 reads in 125.213 CPU sec, 3.953 real sec
sorting block 171, #reads = 1080645 mem = 536878023 peak = 1145675776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283105, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 338, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (354.59, 164.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677504 sequences (100000155 bp) in 3.153 CPU sec, 3.327 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282755, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 338, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (354.87, 164.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677808 reads in 187.496 CPU sec, 5.977 real sec
[M::process] read 677714 sequences (100000071 bp) in 2.389 CPU sec, 2.408 real sec...
[M::mem_process_seqs] Processed 677576 reads in 124.730 CPU sec, 3.943 real sec
sorting block 172, #reads = 1079963 mem = 536879678 peak = 1145675776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282472, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 338, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (354.90, 163.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677832 sequences (100000123 bp) in 2.985 CPU sec, 3.183 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283430, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 335, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (350.83, 160.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677504 reads in 186.983 CPU sec, 5.960 real sec
[M::process] read 677196 sequences (100000089 bp) in 2.221 CPU sec, 2.228 real sec...
sorting block 173, #reads = 1079433 mem = 536880025 peak = 1145675776
[M::mem_process_seqs] Processed 677714 reads in 123.209 CPU sec, 3.884 real sec
sorting block 174, #reads = 1079033 mem = 536879596 peak = 1145675776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283114, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 331, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (347.27, 158.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677424 sequences (100000268 bp) in 2.773 CPU sec, 2.830 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282354, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 334, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (350.97, 161.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677304 sequences (100000128 bp) in 2.180 CPU sec, 2.174 real sec...
[M::mem_process_seqs] Processed 677832 reads in 170.802 CPU sec, 5.388 real sec
[M::mem_process_seqs] Processed 677196 reads in 116.055 CPU sec, 3.662 real sec
sorting block 175, #reads = 1078405 mem = 536879113 peak = 1145675776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283328, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 333, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (350.74, 161.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677508 sequences (100000011 bp) in 2.599 CPU sec, 2.727 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282787, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 332, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (349.56, 161.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677234 sequences (100000089 bp) in 2.373 CPU sec, 2.385 real sec...
[M::mem_process_seqs] Processed 677424 reads in 186.089 CPU sec, 5.900 real sec
[M::mem_process_seqs] Processed 677304 reads in 125.686 CPU sec, 3.974 real sec
sorting block 176, #reads = 1079506 mem = 536880254 peak = 1145675776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282333, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 332, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (350.07, 162.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677320 sequences (100000193 bp) in 3.116 CPU sec, 3.425 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282601, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 331, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (349.10, 160.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677508 reads in 186.676 CPU sec, 5.964 real sec
[M::mem_process_seqs] Processed 677234 reads in 124.449 CPU sec, 3.927 real sec
[M::process] read 677416 sequences (100000097 bp) in 2.549 CPU sec, 2.586 real sec...
sorting block 177, #reads = 1081188 mem = 536883631 peak = 1145675776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282618, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 329, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (347.84, 159.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677590 sequences (100000224 bp) in 3.401 CPU sec, 3.712 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282585, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 329, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (348.76, 162.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677320 reads in 187.470 CPU sec, 5.998 real sec
sorting block 178, #reads = 1081219 mem = 536879026 peak = 1145675776
[M::mem_process_seqs] Processed 677416 reads in 125.705 CPU sec, 3.974 real sec
[M::process] read 677312 sequences (100000030 bp) in 2.969 CPU sec, 3.048 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282173, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 327, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (347.47, 162.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 179, #reads = 1081232 mem = 536880465 peak = 1145675776
[M::process] read 677094 sequences (100000000 bp) in 3.325 CPU sec, 3.507 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282345, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 329, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (349.20, 162.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677590 reads in 183.391 CPU sec, 5.848 real sec
[M::mem_process_seqs] Processed 677312 reads in 120.181 CPU sec, 3.789 real sec
[M::process] read 677230 sequences (100000061 bp) in 3.293 CPU sec, 3.445 real sec...
sorting block 180, #reads = 1080760 mem = 536877380 peak = 1145675776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281694, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 331, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (350.77, 162.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676894 sequences (100000066 bp) in 2.751 CPU sec, 2.813 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282459, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 331, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (349.72, 161.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677094 reads in 173.447 CPU sec, 5.519 real sec
[M::mem_process_seqs] Processed 677230 reads in 115.663 CPU sec, 3.637 real sec
sorting block 181, #reads = 1081216 mem = 536877922 peak = 1145675776
[M::process] read 677028 sequences (100000003 bp) in 2.811 CPU sec, 2.857 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282140, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 332, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (351.06, 162.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677038 sequences (100000010 bp) in 2.347 CPU sec, 2.416 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282676, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 334, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (352.82, 163.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676960 sequences (100000274 bp) in 1.560 CPU sec, 1.558 real sec...
[M::mem_process_seqs] Processed 676894 reads in 171.076 CPU sec, 5.418 real sec
sorting block 182, #reads = 1081270 mem = 536882941 peak = 1145675776
[M::mem_process_seqs] Processed 677028 reads in 111.054 CPU sec, 3.504 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282843, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 333, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (353.54, 165.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676958 sequences (100000236 bp) in 3.133 CPU sec, 3.197 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282826, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 336, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (355.46, 165.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 427962 sequences (63147713 bp) in 1.650 CPU sec, 1.652 real sec...
[M::mem_process_seqs] Processed 677038 reads in 164.811 CPU sec, 5.204 real sec
sorting block 183, #reads = 1081055 mem = 536882005 peak = 1145675776
[M::mem_process_seqs] Processed 676960 reads in 107.761 CPU sec, 3.410 real sec
sorting block 184, #reads = 1081427 mem = 536877643 peak = 1196605440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282264, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 335, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (355.25, 166.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 178798, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 330, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (350.40, 164.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676958 reads in 139.694 CPU sec, 4.530 real sec
[M::mem_process_seqs] Processed 427962 reads in 80.311 CPU sec, 2.604 real sec
sorting block 185, #reads = 1079996 mem = 536882529 peak = 1196605440
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 200444472
overall: 69762818048 mem 28086.321 user 614.109 sys 938.347 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_cVw-T1-TDNA-1_A23MHWWLT4_1.L001\tPU:L001\tSM:659_cVw-T1-TDNA-1_A23MHWWLT4_1\tLB:659_cVw-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_cVw-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_cVw-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 938.385 sec; CPU: 28700.434 sec
sorting block 186, #reads = 221736 mem = 110493096 peak = 1196605440
uniq header count 1
merging 187 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 10543962716
output reads: 201308685
bam_mem_sort: 187 calls 122.512 user 1.643 sys 125.050 real
bam_write: 187 calls 77.056 user 3.988 sys 105.571 real
execute: 1 calls 66.090 user 2.135 sys 1027.715 real
merge_files: 1 calls 66.068 user 2.091 sys 89.171 real
parse_chunk: 2980 calls 179.456 user 38.810 sys 231.324 real
read_chunk: 8027 calls 91.326 user 54.705 sys 908.637 real
sort_block: 1 calls 57.877 user 54.027 sys 865.467 real
write_chunk: 5045 calls 65.654 user 1.666 sys 87.951 real
overall: 3245576192 mem 3504.611 user 125.055 sys 1027.735 real
ls: cannot access '*.cram': No such file or directory