cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_3U6-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. No more license available for sentieon:klib version: sentieon-genomics-202308.03 No more license available for sentieon:klib [M::bwa_idx_load_from_disk] read 3171 ALT contigs No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib [M::main_mem] bwa index loaded in 1703.427 CPU sec, 782.024 real sec [M::process] read 686622 sequences (100000021 bp) in 0.969 CPU sec, 0.978 real sec... [M::process] read 685946 sequences (100000046 bp) in 1.259 CPU sec, 1.252 real sec... [M::process] read 685484 sequences (100000142 bp) in 1.169 CPU sec, 1.175 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 283424, 8, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 288, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (307.71, 200.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 298, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (319.17, 159.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (321, 373, 657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1329) [M::mem_pestat] mean and std.dev: (450.42, 243.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1665) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684340 sequences (100000061 bp) in 1.129 CPU sec, 1.126 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283479, 8, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 314, 1297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3567) [M::mem_pestat] mean and std.dev: (558.53, 516.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 304, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (322.63, 159.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 336, 943) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2419) [M::mem_pestat] mean and std.dev: (524.66, 438.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3157) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 283579, 4, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 361, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1157) [M::mem_pestat] mean and std.dev: (279.92, 161.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 307, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (327.01, 162.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1165) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (253, 358, 696) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1582) [M::mem_pestat] mean and std.dev: (409.59, 253.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2025) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686622 reads in 211.441 CPU sec, 6.728 real sec [M::mem_process_seqs] Processed 685946 reads in 139.994 CPU sec, 4.499 real sec [M::mem_process_seqs] Processed 685484 reads in 151.815 CPU sec, 4.902 real sec sorting block 0, #reads = 1082314 mem = 536880416 peak = 590508032 [M::process] read 682886 sequences (100000085 bp) in 1.699 CPU sec, 1.849 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283033, 8, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 211, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1223) [M::mem_pestat] mean and std.dev: (388.63, 352.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 317, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936) [M::mem_pestat] mean and std.dev: (334.37, 164.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1182) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 345, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (306.11, 92.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683140 sequences (100000018 bp) in 1.241 CPU sec, 1.262 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 282556, 9, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 277, 1627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4491) [M::mem_pestat] mean and std.dev: (613.91, 627.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5923) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 326, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957) [M::mem_pestat] mean and std.dev: (342.16, 166.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1208) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 351, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848) [M::mem_pestat] mean and std.dev: (337.00, 153.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681918 sequences (100000050 bp) in 1.120 CPU sec, 1.125 real sec... [M::mem_process_seqs] Processed 684340 reads in 132.270 CPU sec, 4.197 real sec sorting block 1, #reads = 1082015 mem = 536880618 peak = 918454272 [M::mem_process_seqs] Processed 682886 reads in 83.207 CPU sec, 2.626 real sec sorting block 2, #reads = 1080242 mem = 536880435 peak = 969666560 [M::process] read 681600 sequences (100000083 bp) in 1.406 CPU sec, 1.469 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282480, 5, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (340, 625, 635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1225) [M::mem_pestat] mean and std.dev: (455.17, 230.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 323, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (339.13, 164.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1191) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 330, 710) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1716) [M::mem_pestat] mean and std.dev: (417.74, 302.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2219) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681536 sequences (100000295 bp) in 1.229 CPU sec, 1.237 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 281571, 5, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 332, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797) [M::mem_pestat] mean and std.dev: (282.84, 111.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 998) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 331, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951) [M::mem_pestat] mean and std.dev: (345.33, 165.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1198) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 191, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (188.21, 51.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 281853, 6, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 321, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (284.41, 127.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1180) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 327, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942) [M::mem_pestat] mean and std.dev: (341.95, 164.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1187) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (306, 387, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (134, 564) [M::mem_pestat] mean and std.dev: (359.00, 94.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683140 reads in 193.856 CPU sec, 6.215 real sec [M::mem_process_seqs] Processed 681600 reads in 144.246 CPU sec, 4.635 real sec [M::mem_process_seqs] Processed 681918 reads in 156.178 CPU sec, 5.042 real sec sorting block 3, #reads = 1080500 mem = 536885386 peak = 969666560 [M::process] read 681596 sequences (100000000 bp) in 2.003 CPU sec, 2.171 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 282062, 7, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 242, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (278.31, 106.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 330, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (343.92, 164.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1189) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 246, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028) [M::mem_pestat] mean and std.dev: (332.13, 280.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1454) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 4, #reads = 1079602 mem = 536879801 peak = 969666560 [M::process] read 680830 sequences (100000234 bp) in 1.352 CPU sec, 1.392 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 282207, 6, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 206, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (225.74, 103.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1082) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 325, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (340.59, 162.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1175) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 337, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (257.36, 106.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681536 reads in 139.500 CPU sec, 4.464 real sec [M::mem_process_seqs] Processed 681596 reads in 86.620 CPU sec, 2.738 real sec [M::process] read 682136 sequences (100000006 bp) in 1.391 CPU sec, 1.425 real sec... sorting block 5, #reads = 1079275 mem = 536881084 peak = 969666560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 281585, 9, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 312, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (285.47, 108.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 331, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (346.53, 165.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1191) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 224, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1257) [M::mem_pestat] mean and std.dev: (277.69, 192.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1627) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686360 sequences (100000239 bp) in 1.571 CPU sec, 1.662 real sec... [M::process] read 685630 sequences (100000206 bp) in 1.250 CPU sec, 1.253 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 282243, 5, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 322, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (262.21, 97.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1176) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 327, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (342.43, 165.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1190) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 340, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (332.04, 175.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680830 reads in 136.035 CPU sec, 4.340 real sec [M::mem_process_seqs] Processed 682136 reads in 85.228 CPU sec, 2.718 real sec sorting block 6, #reads = 1079103 mem = 536878564 peak = 969666560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283587, 6, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 402, 544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1286) [M::mem_pestat] mean and std.dev: (381.85, 294.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 303, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (323.96, 162.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (245, 334, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959) [M::mem_pestat] mean and std.dev: (387.11, 204.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1206) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684630 sequences (100000291 bp) in 1.663 CPU sec, 1.779 real sec... [M::process] read 683276 sequences (100000252 bp) in 1.260 CPU sec, 1.255 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 283099, 2, 19) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 309, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (327.47, 161.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1165) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (252, 285, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (307.88, 109.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686360 reads in 136.642 CPU sec, 4.373 real sec [M::mem_process_seqs] Processed 685630 reads in 85.081 CPU sec, 2.726 real sec sorting block 7, #reads = 1080151 mem = 536878239 peak = 969666560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282848, 7, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 377, 1566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4386) [M::mem_pestat] mean and std.dev: (541.83, 648.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5796) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 315, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (332.87, 163.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1185) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (299, 311, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (135, 545) [M::mem_pestat] mean and std.dev: (330.06, 62.37) [M::mem_pestat] low and high boundaries for proper pairs: (53, 627) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682574 sequences (100000096 bp) in 1.868 CPU sec, 1.972 real sec... [M::process] read 682176 sequences (100000039 bp) in 1.290 CPU sec, 1.285 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 282330, 2, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 351, 569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1205) [M::mem_pestat] mean and std.dev: (389.10, 180.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 325, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958) [M::mem_pestat] mean and std.dev: (341.05, 167.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1210) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 270, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (295.67, 143.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684630 reads in 135.751 CPU sec, 4.322 real sec sorting block 8, #reads = 1082368 mem = 536878618 peak = 969666560 [M::mem_process_seqs] Processed 683276 reads in 84.250 CPU sec, 2.686 real sec sorting block 9, #reads = 1080460 mem = 536881923 peak = 969666560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282411, 4, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 181, 562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1364) [M::mem_pestat] mean and std.dev: (280.71, 201.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 330, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965) [M::mem_pestat] mean and std.dev: (344.98, 167.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1218) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (257, 377, 1325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3461) [M::mem_pestat] mean and std.dev: (600.60, 466.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4529) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681630 sequences (100000150 bp) in 1.754 CPU sec, 1.886 real sec... [M::process] read 681210 sequences (100000248 bp) in 1.290 CPU sec, 1.289 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 282497, 6, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 283, 1000) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2668) [M::mem_pestat] mean and std.dev: (446.38, 374.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 329, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959) [M::mem_pestat] mean and std.dev: (343.89, 165.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1210) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 201, 806) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2080) [M::mem_pestat] mean and std.dev: (442.31, 450.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2717) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682574 reads in 138.550 CPU sec, 4.424 real sec [M::mem_process_seqs] Processed 682176 reads in 86.805 CPU sec, 2.759 real sec sorting block 10, #reads = 1080835 mem = 536881387 peak = 969666560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 281874, 11, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 997, 1036) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2698) [M::mem_pestat] mean and std.dev: (684.06, 627.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 333, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949) [M::mem_pestat] mean and std.dev: (346.04, 164.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1195) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1258, 3850, 3850) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9034) [M::mem_pestat] mean and std.dev: (3399.64, 2525.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13501) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 454, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1180) [M::mem_pestat] mean and std.dev: (367.33, 171.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1512) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681092 sequences (100000246 bp) in 2.108 CPU sec, 2.253 real sec... [M::process] read 681568 sequences (100000254 bp) in 1.310 CPU sec, 1.315 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 282119, 3, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 508, 874) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2228) [M::mem_pestat] mean and std.dev: (664.89, 588.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3019) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 332, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949) [M::mem_pestat] mean and std.dev: (346.04, 164.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1195) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 377, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984) [M::mem_pestat] mean and std.dev: (293.60, 120.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1241) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681630 reads in 138.884 CPU sec, 4.419 real sec [M::mem_process_seqs] Processed 681210 reads in 86.429 CPU sec, 2.748 real sec sorting block 11, #reads = 1080013 mem = 536880582 peak = 969666560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 281489, 6, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 287, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (255.18, 85.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 329, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (343.26, 162.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1182) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 336, 634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1506) [M::mem_pestat] mean and std.dev: (386.62, 283.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1942) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681344 sequences (100000090 bp) in 1.895 CPU sec, 2.021 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 281930, 3, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 234, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947) [M::mem_pestat] mean and std.dev: (293.78, 182.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1198) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 683756 sequences (100000288 bp) in 1.340 CPU sec, 1.344 real sec... [M::mem_pestat] (25, 50, 75) percentile: (208, 327, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940) [M::mem_pestat] mean and std.dev: (342.28, 163.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1184) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (292, 346, 578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1150) [M::mem_pestat] mean and std.dev: (328.58, 110.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1436) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681092 reads in 140.064 CPU sec, 4.470 real sec [M::mem_process_seqs] Processed 681568 reads in 87.433 CPU sec, 2.773 real sec sorting block 12, #reads = 1078451 mem = 536878491 peak = 969666560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 282484, 2, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 223, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (229.50, 106.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 329, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (343.81, 164.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1185) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 298, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (303.44, 101.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686534 sequences (100000220 bp) in 2.154 CPU sec, 2.289 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 282782, 5, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 200, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (217.61, 67.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 320, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (337.28, 166.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1185) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 327, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (283.29, 78.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685380 sequences (100000183 bp) in 1.360 CPU sec, 1.361 real sec... [M::mem_process_seqs] Processed 681344 reads in 135.705 CPU sec, 4.316 real sec sorting block 13, #reads = 1078482 mem = 536877463 peak = 969666560 [M::mem_process_seqs] Processed 683756 reads in 84.808 CPU sec, 2.689 real sec sorting block 14, #reads = 1078727 mem = 536877862 peak = 1024114688 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284550, 4, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 190, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (244.36, 113.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 304, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (323.89, 161.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1155) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 280, 578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1386) [M::mem_pestat] mean and std.dev: (452.26, 355.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1876) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684558 sequences (100000013 bp) in 1.817 CPU sec, 1.928 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 283644, 10, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 271, 558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1356) [M::mem_pestat] mean and std.dev: (340.00, 183.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 683396 sequences (100000272 bp) in 1.360 CPU sec, 1.361 real sec... [M::mem_pestat] (25, 50, 75) percentile: (194, 310, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (327.80, 161.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1162) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (431, 431, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (345, 560) [M::mem_pestat] mean and std.dev: (456.00, 32.17) [M::mem_pestat] low and high boundaries for proper pairs: (302, 603) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 307, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (331.39, 129.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686534 reads in 133.750 CPU sec, 4.268 real sec [M::mem_process_seqs] Processed 685380 reads in 84.646 CPU sec, 2.693 real sec sorting block 15, #reads = 1081838 mem = 536878846 peak = 1024114688 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 283759, 6, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 230, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (271.09, 160.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 317, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (334.25, 164.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1186) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (259, 287, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952) [M::mem_pestat] mean and std.dev: (359.31, 141.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1183) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682736 sequences (100000225 bp) in 2.174 CPU sec, 2.310 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 283046, 8, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 227, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (77, 377) [M::mem_pestat] mean and std.dev: (211.54, 39.94) [M::mem_pestat] low and high boundaries for proper pairs: (17, 437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 682640 sequences (100000180 bp) in 1.360 CPU sec, 1.351 real sec... [M::mem_pestat] (25, 50, 75) percentile: (203, 326, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965) [M::mem_pestat] mean and std.dev: (342.23, 167.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1219) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 283, 734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1844) [M::mem_pestat] mean and std.dev: (454.52, 359.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2399) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684558 reads in 136.178 CPU sec, 4.334 real sec sorting block 16, #reads = 1080753 mem = 536878333 peak = 1024114688 [M::process] read 682274 sequences (100000100 bp) in 1.461 CPU sec, 1.471 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282509, 9, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (242, 398, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1127) [M::mem_pestat] mean and std.dev: (333.93, 169.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 330, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967) [M::mem_pestat] mean and std.dev: (345.19, 168.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1221) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (246, 305, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (278.23, 98.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682736 reads in 80.674 CPU sec, 2.789 real sec [M::mem_process_seqs] Processed 683396 reads in 185.571 CPU sec, 6.131 real sec sorting block 17, #reads = 1079096 mem = 536882426 peak = 1024114688 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282526, 1, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (373, 451, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 742) [M::mem_pestat] mean and std.dev: (366.47, 119.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 330, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970) [M::mem_pestat] mean and std.dev: (345.08, 168.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1225) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::process] read 682278 sequences (100000006 bp) in 2.074 CPU sec, 2.216 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 282658, 8, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (264, 356, 709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1599) [M::mem_pestat] mean and std.dev: (369.11, 197.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2044) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 327, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961) [M::mem_pestat] mean and std.dev: (343.46, 166.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1213) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 267, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (307.83, 148.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682244 sequences (100000126 bp) in 1.320 CPU sec, 1.320 real sec... [M::mem_process_seqs] Processed 682640 reads in 152.186 CPU sec, 4.842 real sec [M::mem_process_seqs] Processed 682274 reads in 88.523 CPU sec, 2.810 real sec sorting block 18, #reads = 1081017 mem = 536877525 peak = 1024114688 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 282256, 5, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 325, 551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1351) [M::mem_pestat] mean and std.dev: (355.56, 254.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1751) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 322, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (339.81, 165.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1184) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 260, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1245) [M::mem_pestat] mean and std.dev: (291.67, 128.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1595) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681620 sequences (100000037 bp) in 2.103 CPU sec, 2.239 real sec... [M::process] read 681220 sequences (100000257 bp) in 1.320 CPU sec, 1.324 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282110, 6, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 252, 673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1651) [M::mem_pestat] mean and std.dev: (322.35, 232.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2140) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 324, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940) [M::mem_pestat] mean and std.dev: (340.47, 164.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1185) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (259, 300, 570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1192) [M::mem_pestat] mean and std.dev: (386.38, 209.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1503) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682278 reads in 140.347 CPU sec, 4.467 real sec [M::mem_process_seqs] Processed 682244 reads in 88.002 CPU sec, 2.799 real sec sorting block 19, #reads = 1079113 mem = 536877015 peak = 1024114688 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 282511, 1, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 308, 796) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2042) [M::mem_pestat] mean and std.dev: (466.40, 380.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 329, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949) [M::mem_pestat] mean and std.dev: (344.07, 165.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1196) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (274, 489, 1100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2752) [M::mem_pestat] mean and std.dev: (417.00, 257.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3578) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685716 sequences (100000080 bp) in 2.062 CPU sec, 2.158 real sec... [M::process] read 685658 sequences (100000061 bp) in 1.329 CPU sec, 1.336 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281939, 8, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 252, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978) [M::mem_pestat] mean and std.dev: (283.58, 145.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1244) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 331, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948) [M::mem_pestat] mean and std.dev: (345.98, 166.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1194) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 240, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (282.15, 130.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681620 reads in 136.664 CPU sec, 4.361 real sec sorting block 20, #reads = 1078493 mem = 536880081 peak = 1024114688 [M::mem_process_seqs] Processed 681220 reads in 85.484 CPU sec, 2.723 real sec sorting block 21, #reads = 1079559 mem = 536880608 peak = 1069842432 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 284178, 2, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 377, 630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1504) [M::mem_pestat] mean and std.dev: (374.20, 225.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1941) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 309, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (330.19, 166.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1181) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 259, 1221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3237) [M::mem_pestat] mean and std.dev: (591.31, 532.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4245) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684694 sequences (100000045 bp) in 1.985 CPU sec, 2.148 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 283497, 12, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (252, 355, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (302.16, 123.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 311, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940) [M::mem_pestat] mean and std.dev: (331.29, 166.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1189) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (289, 632, 1439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3739) [M::mem_pestat] mean and std.dev: (771.50, 689.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4889) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 325, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921) [M::mem_pestat] mean and std.dev: (259.57, 111.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1176) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683566 sequences (100000010 bp) in 1.340 CPU sec, 1.340 real sec... [M::mem_process_seqs] Processed 685716 reads in 136.572 CPU sec, 4.353 real sec [M::mem_process_seqs] Processed 685658 reads in 85.389 CPU sec, 2.711 real sec sorting block 22, #reads = 1081093 mem = 536880924 peak = 1069842432 [M::process] read 683258 sequences (100000180 bp) in 1.936 CPU sec, 2.032 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282782, 5, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 213, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (234.90, 111.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 323, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954) [M::mem_pestat] mean and std.dev: (339.84, 167.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1205) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (289, 516, 995) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2407) [M::mem_pestat] mean and std.dev: (626.48, 438.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3113) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682748 sequences (100000293 bp) in 1.330 CPU sec, 1.334 real sec... [M::mem_process_seqs] Processed 683566 reads in 74.618 CPU sec, 2.358 real sec sorting block 23, #reads = 1082217 mem = 536877916 peak = 1069842432 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 283450, 5, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 281, 849) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2149) [M::mem_pestat] mean and std.dev: (407.91, 290.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 317, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950) [M::mem_pestat] mean and std.dev: (335.94, 167.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1201) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 409, 1196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3106) [M::mem_pestat] mean and std.dev: (560.15, 583.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4061) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283079, 2, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (234, 285, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (321.82, 154.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 326, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971) [M::mem_pestat] mean and std.dev: (344.14, 170.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1227) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (253, 321, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (309.80, 130.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682826 sequences (100000154 bp) in 1.664 CPU sec, 1.669 real sec... [M::mem_process_seqs] Processed 684694 reads in 209.804 CPU sec, 6.684 real sec [M::mem_process_seqs] Processed 683258 reads in 98.283 CPU sec, 3.144 real sec sorting block 24, #reads = 1082076 mem = 536880071 peak = 1069842432 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 282520, 5, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 368, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1230) [M::mem_pestat] mean and std.dev: (367.42, 249.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 328, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981) [M::mem_pestat] mean and std.dev: (346.59, 171.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1240) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (139, 247, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1090) [M::mem_pestat] mean and std.dev: (304.31, 229.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1407) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683420 sequences (100000124 bp) in 1.955 CPU sec, 2.094 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 282732, 1, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 291, 583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1267) [M::mem_pestat] mean and std.dev: (423.00, 238.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 326, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965) [M::mem_pestat] mean and std.dev: (343.61, 169.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1219) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 229, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936) [M::mem_pestat] mean and std.dev: (239.58, 102.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1191) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683228 sequences (100000223 bp) in 1.320 CPU sec, 1.317 real sec... [M::mem_process_seqs] Processed 682748 reads in 138.193 CPU sec, 4.415 real sec [M::mem_process_seqs] Processed 682826 reads in 86.699 CPU sec, 2.759 real sec sorting block 25, #reads = 1081230 mem = 536880092 peak = 1069842432 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282909, 2, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 275, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (292.24, 169.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1031) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 320, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957) [M::mem_pestat] mean and std.dev: (339.71, 168.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1209) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 236, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (237.69, 107.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682710 sequences (100000007 bp) in 2.274 CPU sec, 2.435 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282818, 6, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 332, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (275.06, 150.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 320, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942) [M::mem_pestat] mean and std.dev: (338.25, 166.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1189) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (324, 420, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 798) [M::mem_pestat] mean and std.dev: (376.00, 132.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682208 sequences (100000069 bp) in 1.350 CPU sec, 1.346 real sec... [M::mem_process_seqs] Processed 683420 reads in 137.806 CPU sec, 4.387 real sec [M::mem_process_seqs] Processed 683228 reads in 86.286 CPU sec, 2.740 real sec sorting block 26, #reads = 1079868 mem = 536880532 peak = 1069842432 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282557, 4, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 223, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844) [M::mem_pestat] mean and std.dev: (205.08, 93.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1077) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 321, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940) [M::mem_pestat] mean and std.dev: (338.54, 165.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1186) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 376, 855) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2191) [M::mem_pestat] mean and std.dev: (562.81, 469.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2859) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681564 sequences (100000281 bp) in 1.968 CPU sec, 2.106 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282300, 8, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 363, 1483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4123) [M::mem_pestat] mean and std.dev: (877.71, 984.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5443) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 325, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949) [M::mem_pestat] mean and std.dev: (342.37, 167.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1197) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (252, 298, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1077) [M::mem_pestat] mean and std.dev: (347.18, 150.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1352) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689930 sequences (100000251 bp) in 1.410 CPU sec, 1.411 real sec... [M::mem_process_seqs] Processed 682710 reads in 136.186 CPU sec, 4.342 real sec sorting block 27, #reads = 1080334 mem = 536879091 peak = 1069842432 [M::mem_process_seqs] Processed 682208 reads in 85.554 CPU sec, 2.713 real sec sorting block 28, #reads = 1079898 mem = 536882164 peak = 1123557376 [M::process] read 687950 sequences (100000189 bp) in 2.165 CPU sec, 2.305 real sec... [M::process] read 687848 sequences (100000182 bp) in 1.390 CPU sec, 1.389 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 286010, 2, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 286, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (266.43, 119.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 283, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (312.91, 163.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1143) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 288, 542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1254) [M::mem_pestat] mean and std.dev: (350.75, 209.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1610) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689930 reads in 79.255 CPU sec, 2.506 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 282469, 4, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 239, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (246.38, 96.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 328, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954) [M::mem_pestat] mean and std.dev: (344.91, 168.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1203) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1744) [M::mem_pestat] mean and std.dev: (295.30, 211.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2277) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 284771, 3, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 215, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960) [M::mem_pestat] mean and std.dev: (276.94, 170.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1228) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 295, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932) [M::mem_pestat] mean and std.dev: (321.45, 165.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1181) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (348, 360, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (84, 744) [M::mem_pestat] mean and std.dev: (371.38, 145.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685942 sequences (100000022 bp) in 1.449 CPU sec, 1.454 real sec... [M::mem_process_seqs] Processed 681564 reads in 216.546 CPU sec, 6.888 real sec sorting block 29, #reads = 1079866 mem = 536879747 peak = 1123557376 [M::mem_process_seqs] Processed 687950 reads in 101.400 CPU sec, 3.250 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 284580, 1, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 453, 568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1340) [M::mem_pestat] mean and std.dev: (382.69, 215.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1726) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 294, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923) [M::mem_pestat] mean and std.dev: (320.78, 165.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1169) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 309, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1235) [M::mem_pestat] mean and std.dev: (418.00, 315.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1681) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 30, #reads = 1080252 mem = 536878716 peak = 1123557376 [M::process] read 685120 sequences (100000184 bp) in 2.010 CPU sec, 2.152 real sec... [M::process] read 685380 sequences (100000272 bp) in 1.360 CPU sec, 1.365 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 283957, 2, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 291, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053) [M::mem_pestat] mean and std.dev: (265.82, 119.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1345) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 308, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956) [M::mem_pestat] mean and std.dev: (330.65, 167.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1211) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 227, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (261.53, 98.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687848 reads in 143.295 CPU sec, 4.570 real sec [M::mem_process_seqs] Processed 685942 reads in 90.104 CPU sec, 2.863 real sec sorting block 31, #reads = 1081820 mem = 536880605 peak = 1123557376 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283276, 5, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 238, 661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1675) [M::mem_pestat] mean and std.dev: (418.59, 368.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2182) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 314, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965) [M::mem_pestat] mean and std.dev: (334.60, 167.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1222) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (259, 317, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1069) [M::mem_pestat] mean and std.dev: (364.21, 190.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1339) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684002 sequences (100000035 bp) in 1.971 CPU sec, 2.130 real sec... [M::process] read 682580 sequences (100000153 bp) in 1.390 CPU sec, 1.389 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283507, 6, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 305, 3206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9192) [M::mem_pestat] mean and std.dev: (1121.88, 1435.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12185) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 310, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949) [M::mem_pestat] mean and std.dev: (331.04, 166.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1201) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (251, 274, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (169, 374) [M::mem_pestat] mean and std.dev: (271.91, 12.82) [M::mem_pestat] low and high boundaries for proper pairs: (128, 415) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685120 reads in 147.622 CPU sec, 4.703 real sec [M::mem_process_seqs] Processed 685380 reads in 91.532 CPU sec, 2.906 real sec sorting block 32, #reads = 1078714 mem = 536878504 peak = 1123557376 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283213, 6, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 310, 606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1428) [M::mem_pestat] mean and std.dev: (338.14, 198.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1839) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 320, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966) [M::mem_pestat] mean and std.dev: (337.80, 166.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1222) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (247, 313, 1163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2995) [M::mem_pestat] mean and std.dev: (649.53, 634.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3911) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682228 sequences (100000224 bp) in 2.036 CPU sec, 2.192 real sec... [M::process] read 681854 sequences (100000098 bp) in 1.380 CPU sec, 1.380 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 283174, 3, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 230, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028) [M::mem_pestat] mean and std.dev: (270.79, 137.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1306) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 326, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954) [M::mem_pestat] mean and std.dev: (340.48, 163.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1204) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 264, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (252.53, 96.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684002 reads in 145.452 CPU sec, 4.636 real sec sorting block 33, #reads = 1077255 mem = 536882625 peak = 1123557376 [M::process] read 686702 sequences (100000134 bp) in 1.561 CPU sec, 1.567 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282884, 10, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 406, 683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1653) [M::mem_pestat] mean and std.dev: (405.64, 211.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2138) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 330, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960) [M::mem_pestat] mean and std.dev: (344.38, 165.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1211) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (529, 1353, 1353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3001) [M::mem_pestat] mean and std.dev: (1097.40, 578.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3825) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 682228 reads in 84.050 CPU sec, 2.885 real sec [M::mem_process_seqs] Processed 682580 reads in 199.745 CPU sec, 6.565 real sec sorting block 34, #reads = 1076139 mem = 536879404 peak = 1123557376 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 282749, 6, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (307, 363, 646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1324) [M::mem_pestat] mean and std.dev: (473.22, 334.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1811) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 333, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968) [M::mem_pestat] mean and std.dev: (347.51, 167.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1221) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 465, 880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2186) [M::mem_pestat] mean and std.dev: (473.58, 294.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2839) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692052 sequences (100000244 bp) in 1.930 CPU sec, 2.060 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 284418, 7, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 198, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (186.25, 61.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 301, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (324.05, 163.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1169) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 355, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1153) [M::mem_pestat] mean and std.dev: (330.50, 146.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1465) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689064 sequences (100000168 bp) in 1.410 CPU sec, 1.412 real sec... [M::mem_process_seqs] Processed 681854 reads in 159.945 CPU sec, 5.083 real sec [M::mem_process_seqs] Processed 686702 reads in 94.835 CPU sec, 3.000 real sec sorting block 35, #reads = 1076311 mem = 536877204 peak = 1123557376 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 286761, 3, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 179, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1111) [M::mem_pestat] mean and std.dev: (247.64, 139.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 270, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (296.98, 149.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1071) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (238, 313, 596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1312) [M::mem_pestat] mean and std.dev: (397.48, 217.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1670) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686948 sequences (100000210 bp) in 2.116 CPU sec, 2.288 real sec... [M::process] read 686300 sequences (100000281 bp) in 1.400 CPU sec, 1.395 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285745, 5, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (209.82, 90.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 288, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (310.93, 156.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 380, 650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1608) [M::mem_pestat] mean and std.dev: (364.25, 233.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2087) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692052 reads in 152.836 CPU sec, 4.872 real sec sorting block 36, #reads = 1075710 mem = 536880833 peak = 1123557376 [M::mem_process_seqs] Processed 689064 reads in 95.895 CPU sec, 3.043 real sec sorting block 37, #reads = 1076832 mem = 536880031 peak = 1157128192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 284993, 0, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 251, 960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2628) [M::mem_pestat] mean and std.dev: (425.40, 354.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 302, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923) [M::mem_pestat] mean and std.dev: (322.41, 160.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1168) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 371, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (313.94, 158.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1162) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685410 sequences (100000090 bp) in 2.031 CPU sec, 2.175 real sec... [M::process] read 684862 sequences (100000039 bp) in 1.400 CPU sec, 1.401 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 284939, 12, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 223, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (187.31, 75.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 306, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931) [M::mem_pestat] mean and std.dev: (325.04, 160.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1178) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (325, 934, 934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2152) [M::mem_pestat] mean and std.dev: (655.17, 391.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2761) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (319, 394, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841) [M::mem_pestat] mean and std.dev: (370.40, 108.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686948 reads in 147.651 CPU sec, 4.702 real sec [M::mem_process_seqs] Processed 686300 reads in 94.537 CPU sec, 3.001 real sec sorting block 38, #reads = 1077247 mem = 536879387 peak = 1157128192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 284518, 4, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 182, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (259.53, 172.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 311, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (328.78, 161.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1182) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 242, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010) [M::mem_pestat] mean and std.dev: (298.20, 200.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1282) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683064 sequences (100000184 bp) in 2.204 CPU sec, 2.357 real sec... [M::process] read 682346 sequences (100000228 bp) in 1.380 CPU sec, 1.383 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 283959, 0, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 282, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (249.36, 58.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 314, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948) [M::mem_pestat] mean and std.dev: (332.44, 163.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1199) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 234, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (209.56, 66.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685410 reads in 146.466 CPU sec, 4.657 real sec [M::mem_process_seqs] Processed 684862 reads in 92.269 CPU sec, 2.929 real sec sorting block 39, #reads = 1075837 mem = 536883784 peak = 1157128192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282781, 5, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 261, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (242.62, 95.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1165) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 328, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974) [M::mem_pestat] mean and std.dev: (343.13, 167.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1231) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (249, 442, 747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1743) [M::mem_pestat] mean and std.dev: (509.41, 314.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2241) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682722 sequences (100000058 bp) in 2.039 CPU sec, 2.198 real sec... [M::process] read 687306 sequences (100000000 bp) in 1.420 CPU sec, 1.416 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 283045, 3, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 193, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (191.48, 49.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 330, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985) [M::mem_pestat] mean and std.dev: (347.11, 169.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1245) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 274, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (262.81, 129.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683064 reads in 145.363 CPU sec, 4.628 real sec sorting block 40, #reads = 1077167 mem = 536878630 peak = 1157128192 [M::mem_process_seqs] Processed 682346 reads in 91.604 CPU sec, 2.906 real sec sorting block 41, #reads = 1075726 mem = 536878759 peak = 1157128192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 282902, 2, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 327, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1118) [M::mem_pestat] mean and std.dev: (302.82, 128.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 325, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988) [M::mem_pestat] mean and std.dev: (345.60, 172.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1250) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 292, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (332.10, 161.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688998 sequences (100000078 bp) in 2.175 CPU sec, 2.287 real sec... [M::process] read 687168 sequences (100000136 bp) in 1.370 CPU sec, 1.371 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 284814, 13, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 198, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (177.87, 49.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 299, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (323.71, 165.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1183) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (281, 419, 2184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5990) [M::mem_pestat] mean and std.dev: (1126.00, 1555.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7893) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 264, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054) [M::mem_pestat] mean and std.dev: (285.17, 121.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1344) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682722 reads in 150.018 CPU sec, 4.774 real sec [M::mem_process_seqs] Processed 687306 reads in 96.453 CPU sec, 3.067 real sec sorting block 42, #reads = 1072314 mem = 536882597 peak = 1157128192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285118, 4, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 246, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (203.22, 66.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 288, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (313.24, 159.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (260, 376, 1526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4058) [M::mem_pestat] mean and std.dev: (661.86, 614.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5324) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685782 sequences (100000215 bp) in 1.978 CPU sec, 2.106 real sec... [M::process] read 684980 sequences (100000258 bp) in 1.360 CPU sec, 1.368 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 284787, 4, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 220, 560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1284) [M::mem_pestat] mean and std.dev: (307.30, 180.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 300, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (322.33, 162.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1164) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 260, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (269.00, 106.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688998 reads in 140.524 CPU sec, 4.476 real sec [M::mem_process_seqs] Processed 687168 reads in 87.341 CPU sec, 2.775 real sec sorting block 43, #reads = 1081203 mem = 536883875 peak = 1157128192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284324, 5, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (244, 282, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (78, 493) [M::mem_pestat] mean and std.dev: (246.12, 73.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 310, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942) [M::mem_pestat] mean and std.dev: (329.52, 164.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1192) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 329, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (307.00, 106.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684568 sequences (100000171 bp) in 2.189 CPU sec, 2.299 real sec... [M::process] read 683836 sequences (100000223 bp) in 1.370 CPU sec, 1.366 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 283835, 5, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (291, 386, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (101, 576) [M::mem_pestat] mean and std.dev: (328.87, 102.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 315, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951) [M::mem_pestat] mean and std.dev: (333.11, 165.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1203) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 293, 895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2331) [M::mem_pestat] mean and std.dev: (413.12, 322.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3049) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685782 reads in 139.636 CPU sec, 4.442 real sec [M::mem_process_seqs] Processed 684980 reads in 88.219 CPU sec, 2.796 real sec sorting block 44, #reads = 1081377 mem = 536879131 peak = 1157128192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283603, 10, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 198, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (299.76, 233.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1232) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 317, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956) [M::mem_pestat] mean and std.dev: (335.10, 165.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1209) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (178, 178, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (191.44, 101.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 436, 643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1519) [M::mem_pestat] mean and std.dev: (438.48, 277.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1957) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683670 sequences (100000295 bp) in 1.927 CPU sec, 2.028 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283471, 3, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 306, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (271.61, 80.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 683362 sequences (100000016 bp) in 1.390 CPU sec, 1.394 real sec... [M::mem_pestat] (25, 50, 75) percentile: (199, 321, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967) [M::mem_pestat] mean and std.dev: (338.96, 167.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1223) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (283, 312, 551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1087) [M::mem_pestat] mean and std.dev: (394.43, 211.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1355) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684568 reads in 143.070 CPU sec, 4.564 real sec sorting block 45, #reads = 1078542 mem = 536877022 peak = 1157128192 [M::mem_process_seqs] Processed 683836 reads in 90.436 CPU sec, 2.866 real sec sorting block 46, #reads = 1077757 mem = 536877085 peak = 1183690752 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283113, 1, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 231, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1077) [M::mem_pestat] mean and std.dev: (279.20, 194.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1384) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 321, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951) [M::mem_pestat] mean and std.dev: (337.69, 164.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1201) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (255, 431, 771) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1803) [M::mem_pestat] mean and std.dev: (528.36, 377.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2319) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683506 sequences (100000218 bp) in 2.218 CPU sec, 2.320 real sec... [M::process] read 683256 sequences (100000091 bp) in 1.410 CPU sec, 1.410 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283174, 5, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 267, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041) [M::mem_pestat] mean and std.dev: (327.20, 195.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1328) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 320, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950) [M::mem_pestat] mean and std.dev: (337.23, 164.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1200) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (260, 396, 1038) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2594) [M::mem_pestat] mean and std.dev: (718.10, 701.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3522) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683670 reads in 147.347 CPU sec, 4.685 real sec [M::mem_process_seqs] Processed 683362 reads in 92.592 CPU sec, 2.944 real sec sorting block 47, #reads = 1076389 mem = 536879147 peak = 1183690752 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 283310, 5, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 242, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (78, 398) [M::mem_pestat] mean and std.dev: (240.67, 54.87) [M::mem_pestat] low and high boundaries for proper pairs: (14, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 320, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956) [M::mem_pestat] mean and std.dev: (338.53, 166.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1208) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 264, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (258.36, 113.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693190 sequences (100000018 bp) in 1.965 CPU sec, 2.056 real sec... [M::process] read 691242 sequences (100000118 bp) in 1.430 CPU sec, 1.430 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 282693, 3, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (268, 356, 847) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2005) [M::mem_pestat] mean and std.dev: (361.20, 226.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 322, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966) [M::mem_pestat] mean and std.dev: (341.31, 168.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1221) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 518, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1124) [M::mem_pestat] mean and std.dev: (430.07, 212.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1427) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683506 reads in 151.150 CPU sec, 4.814 real sec [M::mem_process_seqs] Processed 683256 reads in 97.504 CPU sec, 3.099 real sec sorting block 48, #reads = 1074587 mem = 536881924 peak = 1183690752 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 287004, 7, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 226, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (237.67, 112.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 266, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844) [M::mem_pestat] mean and std.dev: (295.36, 152.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1068) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 201, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (233.82, 120.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687768 sequences (100000197 bp) in 2.385 CPU sec, 2.515 real sec... [M::process] read 686294 sequences (100000208 bp) in 1.420 CPU sec, 1.416 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 286571, 1, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 309, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1120) [M::mem_pestat] mean and std.dev: (399.20, 271.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 276, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (301.80, 152.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1089) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 259, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (278.84, 123.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693190 reads in 155.351 CPU sec, 4.942 real sec sorting block 49, #reads = 1071576 mem = 536879028 peak = 1183690752 [M::mem_process_seqs] Processed 691242 reads in 96.622 CPU sec, 3.072 real sec sorting block 50, #reads = 1076582 mem = 536878521 peak = 1183690752 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 285159, 5, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 394, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (354.04, 129.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1087) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 294, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (316.15, 157.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 348, 682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1576) [M::mem_pestat] mean and std.dev: (387.86, 235.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2023) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685726 sequences (100000221 bp) in 1.994 CPU sec, 2.099 real sec... [M::process] read 684962 sequences (100000168 bp) in 1.410 CPU sec, 1.417 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 284810, 6, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 372, 808) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1960) [M::mem_pestat] mean and std.dev: (480.09, 289.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 307, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (325.55, 161.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1171) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 347, 515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1083) [M::mem_pestat] mean and std.dev: (346.70, 216.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1367) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687768 reads in 148.195 CPU sec, 4.719 real sec [M::mem_process_seqs] Processed 686294 reads in 93.180 CPU sec, 2.960 real sec sorting block 51, #reads = 1076263 mem = 536880694 peak = 1183690752 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 284634, 3, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 207, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088) [M::mem_pestat] mean and std.dev: (254.92, 131.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1390) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 311, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (330.31, 164.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1197) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 411, 569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1269) [M::mem_pestat] mean and std.dev: (390.00, 191.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1619) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684604 sequences (100000280 bp) in 2.357 CPU sec, 2.484 real sec... [M::process] read 684030 sequences (100000208 bp) in 1.370 CPU sec, 1.379 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 283676, 8, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (337, 441, 1065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2521) [M::mem_pestat] mean and std.dev: (848.44, 754.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3867) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 312, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943) [M::mem_pestat] mean and std.dev: (332.16, 164.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1192) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 277, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (276.06, 106.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685726 reads in 149.237 CPU sec, 4.748 real sec [M::mem_process_seqs] Processed 684962 reads in 94.148 CPU sec, 2.989 real sec sorting block 52, #reads = 1076029 mem = 536879102 peak = 1183690752 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 283635, 9, 20) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 313, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952) [M::mem_pestat] mean and std.dev: (333.62, 166.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1204) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 297, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1126) [M::mem_pestat] mean and std.dev: (336.33, 210.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1441) [M::mem_pestat] skip orientation RR [M::process] read 683428 sequences (100000177 bp) in 1.973 CPU sec, 2.072 real sec... [M::process] read 682574 sequences (100000169 bp) in 1.370 CPU sec, 1.375 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 282969, 3, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 234, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (247.33, 72.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 317, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957) [M::mem_pestat] mean and std.dev: (336.90, 167.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1210) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 320, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053) [M::mem_pestat] mean and std.dev: (354.12, 194.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1338) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684604 reads in 145.910 CPU sec, 4.655 real sec [M::mem_process_seqs] Processed 684030 reads in 91.490 CPU sec, 2.903 real sec sorting block 53, #reads = 1075621 mem = 536878349 peak = 1183690752 [M::process] read 63762 sequences (9316794 bp) in 0.446 CPU sec, 0.458 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 283326, 2, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 255, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (255.00, 126.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 321, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968) [M::mem_pestat] mean and std.dev: (340.65, 169.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1224) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (303, 376, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (101, 606) [M::mem_pestat] mean and std.dev: (352.81, 88.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 283533, 3, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 273, 606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1488) [M::mem_pestat] mean and std.dev: (364.00, 320.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1929) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 326, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981) [M::mem_pestat] mean and std.dev: (346.18, 172.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1240) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 328, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (310.44, 155.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 26290, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 313, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948) [M::mem_pestat] mean and std.dev: (335.14, 167.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1198) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 683428 reads in 143.227 CPU sec, 4.644 real sec sorting block 54, #reads = 1076388 mem = 536883230 peak = 1186680832 [M::mem_process_seqs] Processed 682574 reads in 92.097 CPU sec, 2.981 real sec [M::mem_process_seqs] Processed 63762 reads in 93.451 CPU sec, 3.029 real sec sorting block 55, #reads = 1077699 mem = 536879820 peak = 1204764672 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 60286072 overall: 69883981824 mem 8152.824 user 349.748 sys 1008.782 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_3U6-T1-TDNA-1_A23MHWWLT4_1.L003\tPU:L003\tSM:1136_3U6-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_3U6-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0003.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0003.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 1008.816 sec; CPU: 8502.575 sec sorting block 56, #reads = 198197 mem = 99022544 peak = 1204764672 uniq header count 1 merging 57 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 3557584124 output reads: 60608523 bam_mem_sort: 57 calls 20.464 user 0.227 sys 20.819 real bam_write: 57 calls 46.701 user 1.574 sys 48.625 real execute: 1 calls 11.322 user 0.791 sys 1028.466 real merge_files: 1 calls 11.307 user 0.767 sys 19.330 real parse_chunk: 899 calls 28.442 user 7.222 sys 36.332 real read_chunk: 2427 calls 22.230 user 21.368 sys 226.467 real sort_block: 1 calls 17.155 user 20.752 sys 219.379 real write_chunk: 1526 calls 10.979 user 0.586 sys 18.602 real overall: 2741948416 mem 723.632 user 39.420 sys 1028.487 real ls: cannot access '*.cram': No such file or directory