This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1029_T05-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2738.222 CPU sec, 114.526 real sec [M::process] read 690616 sequences (100000148 bp) in 1.187 CPU sec, 1.189 real sec... [M::process] read 690318 sequences (100000114 bp) in 1.660 CPU sec, 1.660 real sec... [M::process] read 689552 sequences (100000201 bp) in 1.686 CPU sec, 1.697 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281287, 10, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 191, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (230.90, 131.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 285, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (307.20, 152.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1077) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (299, 525, 657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1373) [M::mem_pestat] mean and std.dev: (491.78, 228.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1731) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (139, 377, 617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1573) [M::mem_pestat] mean and std.dev: (328.12, 206.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2051) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689154 sequences (100000114 bp) in 1.646 CPU sec, 1.662 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 281589, 0, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 253, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (300.67, 139.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 287, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (309.50, 153.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1086) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 272, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (275.91, 132.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 281447, 10, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 191, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (197.81, 72.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 291, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (311.21, 153.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1084) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (908, 1943, 1943) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4013) [M::mem_pestat] mean and std.dev: (1611.20, 737.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5048) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 225, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (266.92, 121.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690616 reads in 341.943 CPU sec, 10.992 real sec [M::mem_process_seqs] Processed 690318 reads in 232.965 CPU sec, 7.597 real sec [M::mem_process_seqs] Processed 689552 reads in 257.253 CPU sec, 8.382 real sec sorting block 0, #reads = 1073034 mem = 536877382 peak = 551354368 [M::process] read 688920 sequences (100000174 bp) in 2.577 CPU sec, 2.750 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 280554, 5, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 297, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994) [M::mem_pestat] mean and std.dev: (294.45, 146.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1268) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 293, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (313.47, 154.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (143, 298, 2888) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8378) [M::mem_pestat] mean and std.dev: (1345.86, 1514.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11123) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688414 sequences (100000237 bp) in 2.004 CPU sec, 2.044 real sec... [M::process] read 687988 sequences (100000160 bp) in 1.800 CPU sec, 1.803 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 280552, 9, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 301, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1224) [M::mem_pestat] mean and std.dev: (335.64, 242.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 293, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (313.54, 154.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 255, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (240.38, 72.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689154 reads in 217.685 CPU sec, 6.914 real sec sorting block 1, #reads = 1073018 mem = 536882547 peak = 818741248 [M::mem_process_seqs] Processed 688920 reads in 142.857 CPU sec, 4.554 real sec sorting block 2, #reads = 1071881 mem = 536882494 peak = 820789248 [M::process] read 687560 sequences (100000114 bp) in 1.985 CPU sec, 2.108 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 280309, 8, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 255, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (249.66, 112.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 297, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (317.09, 155.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 248, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008) [M::mem_pestat] mean and std.dev: (303.21, 177.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1285) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687142 sequences (100000130 bp) in 1.932 CPU sec, 1.948 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 280093, 9, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 324, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (273.50, 113.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1178) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 298, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (317.45, 154.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 196, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942) [M::mem_pestat] mean and std.dev: (273.81, 160.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1194) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 279788, 18, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 345, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945) [M::mem_pestat] mean and std.dev: (300.12, 121.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1203) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 300, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (318.50, 154.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1099) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (476, 881, 1749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4295) [M::mem_pestat] mean and std.dev: (948.12, 616.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5568) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 228, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (253.15, 95.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688414 reads in 313.561 CPU sec, 10.180 real sec [M::mem_process_seqs] Processed 687988 reads in 235.266 CPU sec, 7.577 real sec [M::mem_process_seqs] Processed 687560 reads in 259.857 CPU sec, 8.386 real sec sorting block 3, #reads = 1072251 mem = 536878457 peak = 820789248 [M::process] read 687030 sequences (100000111 bp) in 2.655 CPU sec, 2.795 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 280240, 10, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 345, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (359.00, 248.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1352) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 303, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (321.76, 156.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (355, 609, 1312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3226) [M::mem_pestat] mean and std.dev: (918.90, 837.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4269) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 271, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (275.62, 133.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 4, #reads = 1071192 mem = 536878351 peak = 820789248 [M::process] read 686386 sequences (100000074 bp) in 2.271 CPU sec, 2.421 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 280072, 4, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 472, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1121) [M::mem_pestat] mean and std.dev: (332.69, 152.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 304, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (322.71, 157.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (272, 311, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (24, 644) [M::mem_pestat] mean and std.dev: (294.12, 101.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685558 sequences (100000076 bp) in 1.856 CPU sec, 1.863 real sec... [M::mem_process_seqs] Processed 687142 reads in 224.762 CPU sec, 7.154 real sec [M::mem_process_seqs] Processed 687030 reads in 146.415 CPU sec, 4.647 real sec sorting block 5, #reads = 1070835 mem = 536879887 peak = 820789248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 279820, 8, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 316, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (305.63, 161.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 306, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (325.14, 158.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 512, 976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2458) [M::mem_pestat] mean and std.dev: (643.64, 579.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3199) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685920 sequences (100000163 bp) in 2.717 CPU sec, 2.878 real sec... [M::process] read 686058 sequences (100000253 bp) in 1.480 CPU sec, 1.487 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 279806, 11, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 279, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999) [M::mem_pestat] mean and std.dev: (317.53, 215.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1274) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 311, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (328.64, 158.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (649, 1590, 1590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3472) [M::mem_pestat] mean and std.dev: (1081.70, 539.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4413) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 320, 607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1403) [M::mem_pestat] mean and std.dev: (352.54, 194.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1801) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686386 reads in 217.841 CPU sec, 6.960 real sec [M::mem_process_seqs] Processed 685558 reads in 144.366 CPU sec, 4.616 real sec sorting block 6, #reads = 1070059 mem = 536880788 peak = 820789248 [M::process] read 685624 sequences (100000189 bp) in 2.660 CPU sec, 2.790 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 279641, 10, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (240, 276, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (291.68, 130.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 308, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (326.13, 157.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (378, 1242, 1242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2970) [M::mem_pestat] mean and std.dev: (745.38, 423.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3834) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (285, 356, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963) [M::mem_pestat] mean and std.dev: (374.83, 181.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1189) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686016 sequences (100000234 bp) in 2.004 CPU sec, 2.007 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 279256, 12, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 253, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (266.68, 109.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 306, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (325.41, 157.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (682, 682, 1763) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3925) [M::mem_pestat] mean and std.dev: (989.08, 780.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5006) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 319, 610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1412) [M::mem_pestat] mean and std.dev: (355.81, 193.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1813) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 279182, 7, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 326, 566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1300) [M::mem_pestat] mean and std.dev: (354.93, 228.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 309, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (326.39, 156.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (251, 296, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028) [M::mem_pestat] mean and std.dev: (360.05, 213.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1287) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685920 reads in 319.627 CPU sec, 10.217 real sec sorting block 7, #reads = 1069575 mem = 536884677 peak = 822210560 [M::mem_process_seqs] Processed 686058 reads in 245.324 CPU sec, 7.870 real sec [M::mem_process_seqs] Processed 685624 reads in 270.393 CPU sec, 8.697 real sec sorting block 8, #reads = 1069676 mem = 536876178 peak = 822210560 [M::process] read 686018 sequences (100000212 bp) in 2.876 CPU sec, 3.065 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 279663, 9, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 275, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (263.96, 127.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1030) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 308, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (325.74, 156.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 310, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1131) [M::mem_pestat] mean and std.dev: (347.04, 189.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1440) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686248 sequences (100000196 bp) in 2.499 CPU sec, 2.617 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 279537, 16, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 197, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1071) [M::mem_pestat] mean and std.dev: (289.56, 200.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 308, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (327.05, 159.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (476, 867, 1139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2465) [M::mem_pestat] mean and std.dev: (724.69, 421.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3128) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 321, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (325.03, 136.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686102 sequences (100000164 bp) in 1.889 CPU sec, 1.900 real sec... [M::mem_process_seqs] Processed 686016 reads in 232.539 CPU sec, 7.428 real sec sorting block 9, #reads = 1069351 mem = 536880814 peak = 822210560 [M::mem_process_seqs] Processed 686018 reads in 152.103 CPU sec, 4.838 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 279401, 8, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 223, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (37, 367) [M::mem_pestat] mean and std.dev: (205.93, 48.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 305, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (324.38, 157.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (261, 334, 609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1305) [M::mem_pestat] mean and std.dev: (396.47, 268.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1653) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685550 sequences (100000187 bp) in 2.750 CPU sec, 2.919 real sec... [M::process] read 685062 sequences (100000066 bp) in 2.031 CPU sec, 2.036 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 279807, 11, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (281, 382, 682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1484) [M::mem_pestat] mean and std.dev: (437.82, 242.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1885) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 306, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (324.87, 157.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1123) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (801, 1079, 2663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6387) [M::mem_pestat] mean and std.dev: (1526.45, 1146.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8249) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 303, 811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2013) [M::mem_pestat] mean and std.dev: (411.21, 308.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2614) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686248 reads in 224.793 CPU sec, 7.238 real sec sorting block 10, #reads = 1069445 mem = 536880178 peak = 822210560 [M::mem_process_seqs] Processed 686102 reads in 143.884 CPU sec, 4.578 real sec sorting block 11, #reads = 1069777 mem = 536879667 peak = 822210560 [M::process] read 684858 sequences (100000026 bp) in 2.329 CPU sec, 2.451 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 279658, 2, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 237, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (262.03, 153.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 309, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (327.59, 157.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 386, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1120) [M::mem_pestat] mean and std.dev: (399.37, 219.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1417) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684734 sequences (100000103 bp) in 2.035 CPU sec, 2.051 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 278728, 9, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 326, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040) [M::mem_pestat] mean and std.dev: (326.74, 178.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1323) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 308, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (325.57, 154.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 248, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1138) [M::mem_pestat] mean and std.dev: (329.06, 201.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1465) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 279058, 6, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 311, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (285.24, 156.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 311, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (329.10, 158.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1138) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 279, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (318.02, 126.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685550 reads in 305.496 CPU sec, 9.754 real sec [M::mem_process_seqs] Processed 684858 reads in 231.985 CPU sec, 7.441 real sec [M::mem_process_seqs] Processed 685062 reads in 255.014 CPU sec, 8.221 real sec sorting block 12, #reads = 1069808 mem = 536879323 peak = 822210560 [M::process] read 685264 sequences (100000074 bp) in 3.073 CPU sec, 3.372 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 278662, 11, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 249, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (256.00, 165.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1072) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 309, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (326.67, 156.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (269, 746, 2590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7232) [M::mem_pestat] mean and std.dev: (1162.82, 1084.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9553) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 250, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1152) [M::mem_pestat] mean and std.dev: (326.24, 225.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1473) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 13, #reads = 1068644 mem = 536883534 peak = 822210560 [M::process] read 685512 sequences (100000214 bp) in 2.265 CPU sec, 2.329 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 278632, 12, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 328, 601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1313) [M::mem_pestat] mean and std.dev: (321.47, 165.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 305, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (323.14, 153.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1104) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (210, 331, 1397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3771) [M::mem_pestat] mean and std.dev: (622.83, 568.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4958) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (246, 293, 675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1533) [M::mem_pestat] mean and std.dev: (421.59, 255.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1962) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684734 reads in 214.241 CPU sec, 6.871 real sec [M::process] read 685300 sequences (100000294 bp) in 2.038 CPU sec, 2.072 real sec... [M::mem_process_seqs] Processed 685264 reads in 139.871 CPU sec, 4.600 real sec sorting block 14, #reads = 1069199 mem = 536878109 peak = 822210560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 279123, 9, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 311, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056) [M::mem_pestat] mean and std.dev: (326.70, 224.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1364) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 307, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (324.62, 155.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 365, 659) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1509) [M::mem_pestat] mean and std.dev: (386.94, 280.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1934) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684744 sequences (100000068 bp) in 2.743 CPU sec, 2.937 real sec... [M::process] read 685470 sequences (100000079 bp) in 1.980 CPU sec, 1.982 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 278735, 11, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 381, 625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1463) [M::mem_pestat] mean and std.dev: (335.19, 202.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1882) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 307, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (324.22, 154.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (503, 553, 1610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3824) [M::mem_pestat] mean and std.dev: (1025.09, 635.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4931) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 264, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (254.18, 97.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685512 reads in 217.648 CPU sec, 7.077 real sec [M::mem_process_seqs] Processed 685300 reads in 136.264 CPU sec, 4.415 real sec sorting block 15, #reads = 1068235 mem = 536878278 peak = 822210560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 278019, 4, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 233, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (239.62, 112.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1088) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 309, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (326.47, 155.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 244, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (288.22, 151.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685614 sequences (100000155 bp) in 2.738 CPU sec, 2.895 real sec... [M::process] read 685276 sequences (100000086 bp) in 2.040 CPU sec, 2.039 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 279183, 6, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 252, 858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2204) [M::mem_pestat] mean and std.dev: (539.77, 527.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2877) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 305, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (322.92, 154.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1104) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 377, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054) [M::mem_pestat] mean and std.dev: (336.14, 132.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1339) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684744 reads in 214.495 CPU sec, 6.892 real sec sorting block 16, #reads = 1067223 mem = 536879486 peak = 822210560 [M::mem_process_seqs] Processed 685470 reads in 138.813 CPU sec, 4.414 real sec sorting block 17, #reads = 1067560 mem = 536879274 peak = 822210560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 279341, 13, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 477, 685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1609) [M::mem_pestat] mean and std.dev: (455.28, 325.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2071) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 305, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (323.22, 155.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (561, 561, 863) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1467) [M::mem_pestat] mean and std.dev: (609.15, 262.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1769) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 299, 567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1271) [M::mem_pestat] mean and std.dev: (361.46, 254.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1623) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684974 sequences (100000167 bp) in 2.841 CPU sec, 3.051 real sec... [M::process] read 685336 sequences (100000064 bp) in 2.020 CPU sec, 2.012 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 278798, 17, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 305, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (270.29, 152.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 306, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (324.41, 155.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (279, 504, 902) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2148) [M::mem_pestat] mean and std.dev: (583.81, 585.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2925) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 260, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1069) [M::mem_pestat] mean and std.dev: (286.53, 132.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1359) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685614 reads in 217.372 CPU sec, 6.958 real sec [M::mem_process_seqs] Processed 685276 reads in 138.224 CPU sec, 4.468 real sec sorting block 18, #reads = 1068488 mem = 536880980 peak = 822210560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 278946, 3, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 250, 1142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3112) [M::mem_pestat] mean and std.dev: (325.00, 266.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4097) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 307, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (324.74, 154.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (273, 303, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 669) [M::mem_pestat] mean and std.dev: (297.23, 105.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685202 sequences (100000013 bp) in 2.666 CPU sec, 2.817 real sec... [M::process] read 685192 sequences (100000136 bp) in 2.040 CPU sec, 2.038 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 279576, 9, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 351, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (314.06, 145.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1090) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 306, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (324.36, 155.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 270, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (239.20, 98.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684974 reads in 209.410 CPU sec, 6.725 real sec [M::mem_process_seqs] Processed 685336 reads in 136.234 CPU sec, 4.413 real sec sorting block 19, #reads = 1066631 mem = 536876635 peak = 822210560 [M::process] read 684566 sequences (100000164 bp) in 2.425 CPU sec, 2.585 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 279702, 9, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 244, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (305.76, 186.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1088) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 306, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (325.28, 156.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 221, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948) [M::mem_pestat] mean and std.dev: (302.82, 209.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1209) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684236 sequences (100000000 bp) in 1.960 CPU sec, 2.002 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278819, 17, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 277, 566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1326) [M::mem_pestat] mean and std.dev: (284.79, 159.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 312, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (330.92, 159.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (952, 1173, 2779) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6433) [M::mem_pestat] mean and std.dev: (1624.87, 1456.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8260) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 321, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940) [M::mem_pestat] mean and std.dev: (298.76, 128.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1189) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685202 reads in 202.576 CPU sec, 6.543 real sec sorting block 20, #reads = 1068592 mem = 536879504 peak = 822210560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 279363, 9, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 206, 865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2261) [M::mem_pestat] mean and std.dev: (491.61, 487.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2959) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 310, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (328.56, 158.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 320, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (269.42, 92.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686192 sequences (100000047 bp) in 2.215 CPU sec, 2.217 real sec... [M::mem_process_seqs] Processed 684566 reads in 222.034 CPU sec, 7.047 real sec [M::mem_process_seqs] Processed 685192 reads in 244.459 CPU sec, 8.052 real sec sorting block 21, #reads = 1066908 mem = 536884310 peak = 822210560 [M::process] read 690310 sequences (100000098 bp) in 2.666 CPU sec, 2.833 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 278562, 8, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940) [M::mem_pestat] mean and std.dev: (259.76, 121.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1199) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 313, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (331.86, 160.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (259, 350, 727) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1663) [M::mem_pestat] mean and std.dev: (568.08, 462.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2419) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684236 reads in 111.768 CPU sec, 3.849 real sec sorting block 22, #reads = 1067546 mem = 536883498 peak = 822210560 [M::process] read 689258 sequences (100000019 bp) in 2.302 CPU sec, 2.566 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 280288, 5, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 302, 898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2380) [M::mem_pestat] mean and std.dev: (322.05, 229.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3121) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 305, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (324.61, 158.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (139, 173, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (220.67, 149.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688816 sequences (100000013 bp) in 1.950 CPU sec, 1.948 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 281357, 4, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 245, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (240.00, 92.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 286, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846) [M::mem_pestat] mean and std.dev: (307.24, 151.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1066) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 332, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1211) [M::mem_pestat] mean and std.dev: (356.64, 181.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1545) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686192 reads in 223.661 CPU sec, 7.140 real sec [M::mem_process_seqs] Processed 690310 reads in 239.864 CPU sec, 7.738 real sec sorting block 23, #reads = 1067772 mem = 536880779 peak = 822210560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 280917, 6, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 305, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035) [M::mem_pestat] mean and std.dev: (270.70, 135.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1315) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 290, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (311.78, 154.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1088) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 240, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (245.26, 94.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688264 sequences (100000093 bp) in 2.728 CPU sec, 2.918 real sec... [M::process] read 687922 sequences (100000212 bp) in 1.920 CPU sec, 1.914 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 280608, 13, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 370, 614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1496) [M::mem_pestat] mean and std.dev: (492.72, 374.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1991) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 293, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (312.60, 153.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1086) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (92, 350, 1006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2834) [M::mem_pestat] mean and std.dev: (558.85, 599.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3748) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 226, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (237.88, 104.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689258 reads in 189.987 CPU sec, 6.112 real sec [M::mem_process_seqs] Processed 688816 reads in 119.698 CPU sec, 3.797 real sec sorting block 24, #reads = 1071968 mem = 536885754 peak = 822210560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 281287, 9, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 183, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (161.89, 44.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 295, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (314.36, 153.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1088) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 208, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (269.19, 152.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687654 sequences (100000252 bp) in 2.614 CPU sec, 2.751 real sec... [M::process] read 687318 sequences (100000249 bp) in 1.949 CPU sec, 1.947 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 280722, 3, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 243, 626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1588) [M::mem_pestat] mean and std.dev: (415.78, 366.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2069) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 296, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (314.30, 152.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1081) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 362, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1389) [M::mem_pestat] mean and std.dev: (361.32, 209.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1804) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688264 reads in 178.084 CPU sec, 5.647 real sec sorting block 25, #reads = 1073551 mem = 536878545 peak = 822210560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 279985, 9, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 192, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (213.17, 135.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 298, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (315.98, 152.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1086) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 287, 754) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1874) [M::mem_pestat] mean and std.dev: (400.29, 305.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2434) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687032 sequences (100000105 bp) in 1.986 CPU sec, 1.995 real sec... [M::mem_process_seqs] Processed 687654 reads in 95.395 CPU sec, 3.025 real sec sorting block 26, #reads = 1072526 mem = 536879758 peak = 822210560 [M::mem_process_seqs] Processed 687922 reads in 229.820 CPU sec, 7.294 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 280125, 12, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 293, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849) [M::mem_pestat] mean and std.dev: (275.08, 131.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 299, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (317.43, 153.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1091) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (451, 1509, 1548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3742) [M::mem_pestat] mean and std.dev: (1127.08, 769.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4839) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 196, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (249.96, 155.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1089) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687174 sequences (100000178 bp) in 2.522 CPU sec, 2.667 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 280100, 4, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 316, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976) [M::mem_pestat] mean and std.dev: (318.10, 166.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1244) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 302, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (319.00, 153.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1096) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 383, 847) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2107) [M::mem_pestat] mean and std.dev: (583.50, 548.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2777) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687074 sequences (100000292 bp) in 1.500 CPU sec, 1.499 real sec... [M::mem_process_seqs] Processed 687318 reads in 185.206 CPU sec, 5.885 real sec sorting block 27, #reads = 1071990 mem = 536879187 peak = 822210560 [M::mem_process_seqs] Processed 687032 reads in 107.427 CPU sec, 3.414 real sec sorting block 28, #reads = 1072630 mem = 536881124 peak = 822210560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 280247, 15, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 281, 1249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3423) [M::mem_pestat] mean and std.dev: (710.66, 760.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 301, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (319.00, 153.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1096) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (320, 512, 913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2099) [M::mem_pestat] mean and std.dev: (622.80, 459.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2692) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 228, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (266.62, 131.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686830 sequences (100000286 bp) in 2.627 CPU sec, 2.824 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 279917, 7, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 306, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (297.92, 116.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1075) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 305, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (321.98, 155.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (249, 284, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 609) [M::mem_pestat] mean and std.dev: (271.45, 93.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686494 sequences (100000144 bp) in 1.880 CPU sec, 1.882 real sec... [M::mem_process_seqs] Processed 687174 reads in 170.345 CPU sec, 5.420 real sec [M::mem_process_seqs] Processed 687074 reads in 108.664 CPU sec, 3.524 real sec sorting block 29, #reads = 1071483 mem = 536876444 peak = 822210560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 280164, 2, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 353, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (318.34, 123.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 304, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (321.62, 155.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 293, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (285.45, 122.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1069) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686496 sequences (100000044 bp) in 2.581 CPU sec, 2.711 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 279587, 7, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 214, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973) [M::mem_pestat] mean and std.dev: (249.61, 144.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1245) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 306, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (323.63, 156.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 234, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (270.57, 152.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686084 sequences (100000124 bp) in 1.930 CPU sec, 1.931 real sec... [M::mem_process_seqs] Processed 686830 reads in 172.167 CPU sec, 5.541 real sec [M::mem_process_seqs] Processed 686494 reads in 108.590 CPU sec, 3.448 real sec sorting block 30, #reads = 1070329 mem = 536878993 peak = 822210560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 279924, 5, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 205, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (273.41, 208.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 306, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (323.11, 155.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 292, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (276.03, 121.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1086) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685948 sequences (100000152 bp) in 2.564 CPU sec, 2.720 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 279226, 7, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 256, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (226.47, 80.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 307, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (324.06, 156.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 305, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968) [M::mem_pestat] mean and std.dev: (325.63, 202.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1229) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685920 sequences (100000296 bp) in 1.940 CPU sec, 1.942 real sec... [M::mem_process_seqs] Processed 686496 reads in 172.873 CPU sec, 5.560 real sec sorting block 31, #reads = 1069770 mem = 536877827 peak = 822210560 [M::mem_process_seqs] Processed 686084 reads in 109.449 CPU sec, 3.535 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 279965, 4, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 388, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036) [M::mem_pestat] mean and std.dev: (353.58, 209.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1314) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 309, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (326.08, 156.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 287, 664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1536) [M::mem_pestat] mean and std.dev: (311.90, 160.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1972) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685894 sequences (100000166 bp) in 2.642 CPU sec, 2.799 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 279427, 8, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 293, 712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1694) [M::mem_pestat] mean and std.dev: (501.94, 474.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2400) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 309, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (325.91, 156.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 337, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (315.69, 117.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685482 sequences (100000267 bp) in 1.900 CPU sec, 1.895 real sec... [M::mem_process_seqs] Processed 685948 reads in 168.186 CPU sec, 5.423 real sec sorting block 32, #reads = 1070382 mem = 536879192 peak = 822210560 [M::mem_process_seqs] Processed 685920 reads in 104.491 CPU sec, 3.339 real sec sorting block 33, #reads = 1071331 mem = 536878763 peak = 822210560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 280475, 6, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 275, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (270.92, 114.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 308, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (324.79, 155.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 341, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991) [M::mem_pestat] mean and std.dev: (346.79, 172.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1252) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685262 sequences (100000166 bp) in 2.632 CPU sec, 2.746 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 279710, 20, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 237, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (67, 367) [M::mem_pestat] mean and std.dev: (220.61, 56.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 311, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (327.97, 157.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (93, 97, 790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2184) [M::mem_pestat] mean and std.dev: (332.33, 519.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2881) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 303, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (297.00, 96.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685894 reads in 161.768 CPU sec, 5.156 real sec [M::process] read 685250 sequences (100000155 bp) in 1.880 CPU sec, 1.889 real sec... [M::mem_process_seqs] Processed 685482 reads in 107.469 CPU sec, 3.386 real sec sorting block 34, #reads = 1070766 mem = 536878210 peak = 822210560 [M::process] read 685532 sequences (100000086 bp) in 2.658 CPU sec, 2.792 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 279296, 9, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 292, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (275.12, 118.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 306, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (322.08, 152.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1089) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 250, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (257.97, 111.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685070 sequences (100000236 bp) in 1.890 CPU sec, 1.891 real sec... [M::mem_process_seqs] Processed 685250 reads in 95.216 CPU sec, 2.994 real sec sorting block 35, #reads = 1069241 mem = 536878486 peak = 822210560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 279447, 13, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 387, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1102) [M::mem_pestat] mean and std.dev: (352.91, 178.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 310, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (327.79, 157.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (192, 937, 937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2427) [M::mem_pestat] mean and std.dev: (618.83, 387.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3172) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 276, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (316.67, 159.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1084) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 280091, 12, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (306, 570, 1002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2394) [M::mem_pestat] mean and std.dev: (553.20, 445.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3090) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 309, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (326.61, 156.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1249, 1249, 4412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10738) [M::mem_pestat] mean and std.dev: (2429.58, 2443.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13901) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 308, 895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2275) [M::mem_pestat] mean and std.dev: (441.76, 355.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2965) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684876 sequences (100000194 bp) in 2.049 CPU sec, 2.058 real sec... [M::mem_process_seqs] Processed 685262 reads in 260.005 CPU sec, 8.327 real sec [M::mem_process_seqs] Processed 685532 reads in 129.382 CPU sec, 4.114 real sec sorting block 36, #reads = 1070669 mem = 536879203 peak = 822210560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 279989, 12, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 302, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1087) [M::mem_pestat] mean and std.dev: (358.52, 218.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1374) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 312, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (329.05, 157.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1102, 1953, 1953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3655) [M::mem_pestat] mean and std.dev: (1493.58, 520.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4506) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 200, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052) [M::mem_pestat] mean and std.dev: (289.51, 206.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1351) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684714 sequences (100000138 bp) in 2.562 CPU sec, 2.763 real sec... [M::process] read 685006 sequences (100000252 bp) in 1.440 CPU sec, 1.446 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278990, 8, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 280, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (264.24, 125.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 309, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (325.68, 154.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 283, 599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1421) [M::mem_pestat] mean and std.dev: (306.86, 202.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1832) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685070 reads in 176.058 CPU sec, 5.687 real sec [M::mem_process_seqs] Processed 684876 reads in 113.041 CPU sec, 3.678 real sec sorting block 37, #reads = 1069815 mem = 536878871 peak = 822210560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 278933, 18, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 193, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (216.65, 85.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 312, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (328.77, 156.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (371, 996, 1114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2600) [M::mem_pestat] mean and std.dev: (853.59, 725.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3754) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 284, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (219.32, 86.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686858 sequences (100000276 bp) in 2.854 CPU sec, 3.091 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 279462, 13, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 294, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (263.45, 88.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 309, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (327.74, 157.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (912, 1148, 3231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7869) [M::mem_pestat] mean and std.dev: (1891.54, 1792.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10188) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 256, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036) [M::mem_pestat] mean and std.dev: (280.59, 133.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1323) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684832 sequences (100000226 bp) in 1.930 CPU sec, 1.930 real sec... [M::mem_process_seqs] Processed 684714 reads in 182.067 CPU sec, 5.855 real sec sorting block 38, #reads = 1069761 mem = 536877070 peak = 822210560 [M::mem_process_seqs] Processed 685006 reads in 118.000 CPU sec, 3.832 real sec sorting block 39, #reads = 1069187 mem = 536882666 peak = 822210560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 279840, 15, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 230, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (280.81, 164.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1084) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 299, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (319.25, 155.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1100) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (747, 934, 2093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4785) [M::mem_pestat] mean and std.dev: (1524.50, 1362.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6973) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 257, 506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1102) [M::mem_pestat] mean and std.dev: (276.44, 162.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1400) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685260 sequences (100000067 bp) in 2.648 CPU sec, 2.791 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 279563, 4, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 252, 653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1659) [M::mem_pestat] mean and std.dev: (331.24, 257.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2162) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 312, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (329.29, 157.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 232, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (239.23, 74.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685374 sequences (100000112 bp) in 1.940 CPU sec, 1.938 real sec... [M::mem_process_seqs] Processed 686858 reads in 178.927 CPU sec, 5.764 real sec [M::mem_process_seqs] Processed 684832 reads in 112.370 CPU sec, 3.555 real sec sorting block 40, #reads = 1068481 mem = 536881701 peak = 822210560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 279785, 6, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 220, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (228.03, 117.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 307, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (325.56, 156.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 263, 786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1988) [M::mem_pestat] mean and std.dev: (375.19, 325.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2589) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685346 sequences (100000110 bp) in 2.752 CPU sec, 2.935 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278961, 12, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 259, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (269.24, 127.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 306, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (324.31, 155.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (726, 1194, 1310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2478) [M::mem_pestat] mean and std.dev: (973.64, 518.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3062) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 300, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (262.56, 104.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685266 sequences (100000086 bp) in 1.860 CPU sec, 1.859 real sec... [M::mem_process_seqs] Processed 685260 reads in 172.484 CPU sec, 5.532 real sec [M::mem_process_seqs] Processed 685374 reads in 110.586 CPU sec, 3.489 real sec sorting block 41, #reads = 1068819 mem = 536884333 peak = 822210560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 279649, 11, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 213, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (221.41, 129.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 307, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (325.49, 156.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (65, 325, 2156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6338) [M::mem_pestat] mean and std.dev: (744.55, 893.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8429) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 330, 687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1611) [M::mem_pestat] mean and std.dev: (455.00, 343.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2073) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685138 sequences (100000197 bp) in 2.752 CPU sec, 2.928 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 278803, 16, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 206, 1018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2690) [M::mem_pestat] mean and std.dev: (426.77, 425.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 306, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (324.40, 156.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (221, 1106, 5653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16517) [M::mem_pestat] mean and std.dev: (2051.69, 2577.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21949) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 248, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1174) [M::mem_pestat] mean and std.dev: (284.60, 257.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1515) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685346 reads in 162.036 CPU sec, 5.203 real sec [M::process] read 685130 sequences (100000190 bp) in 1.937 CPU sec, 1.953 real sec... [M::mem_process_seqs] Processed 685266 reads in 106.658 CPU sec, 3.362 real sec sorting block 42, #reads = 1067736 mem = 536879085 peak = 822210560 [M::process] read 685040 sequences (100000192 bp) in 2.648 CPU sec, 2.793 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 279258, 12, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 224, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (276.05, 155.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 307, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (325.66, 156.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (906, 1027, 2842) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6714) [M::mem_pestat] mean and std.dev: (1787.92, 1713.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8650) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 256, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031) [M::mem_pestat] mean and std.dev: (317.23, 222.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1320) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685250 sequences (100000218 bp) in 1.900 CPU sec, 1.897 real sec... [M::mem_process_seqs] Processed 685130 reads in 94.859 CPU sec, 2.983 real sec sorting block 43, #reads = 1067783 mem = 536881031 peak = 822210560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 278824, 5, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 213, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1110) [M::mem_pestat] mean and std.dev: (304.60, 240.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 307, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (325.52, 156.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 280, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025) [M::mem_pestat] mean and std.dev: (273.11, 122.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1291) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 279444, 9, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 320, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1196) [M::mem_pestat] mean and std.dev: (342.47, 249.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 309, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (327.98, 158.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 267, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075) [M::mem_pestat] mean and std.dev: (251.72, 127.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1371) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684986 sequences (100000008 bp) in 2.083 CPU sec, 2.092 real sec... [M::mem_process_seqs] Processed 685138 reads in 258.405 CPU sec, 8.377 real sec [M::mem_process_seqs] Processed 685040 reads in 126.492 CPU sec, 4.024 real sec sorting block 44, #reads = 1066773 mem = 536882026 peak = 871153664 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 279152, 12, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 351, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (308.26, 137.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 310, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (329.40, 160.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (419, 1827, 1827) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4643) [M::mem_pestat] mean and std.dev: (1196.58, 757.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6051) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 266, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (252.52, 81.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685168 sequences (100000224 bp) in 2.708 CPU sec, 2.906 real sec... [M::process] read 689674 sequences (100000109 bp) in 1.480 CPU sec, 1.473 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 279724, 8, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (227, 401, 840) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2066) [M::mem_pestat] mean and std.dev: (585.88, 448.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 311, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (331.45, 161.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 248, 641) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1535) [M::mem_pestat] mean and std.dev: (360.42, 280.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1982) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685250 reads in 163.033 CPU sec, 5.178 real sec sorting block 45, #reads = 1068294 mem = 536883337 peak = 871153664 [M::mem_process_seqs] Processed 684986 reads in 111.676 CPU sec, 3.563 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 279157, 6, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 331, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088) [M::mem_pestat] mean and std.dev: (374.84, 254.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1392) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 312, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (331.67, 161.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 298, 617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1549) [M::mem_pestat] mean and std.dev: (379.81, 325.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2015) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 46, #reads = 1068744 mem = 536879774 peak = 871153664 [M::process] read 689728 sequences (100000286 bp) in 2.535 CPU sec, 2.794 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 280985, 5, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 271, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (317.82, 205.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 289, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (308.98, 151.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1071) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 393, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1150) [M::mem_pestat] mean and std.dev: (349.11, 167.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1464) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688716 sequences (100000118 bp) in 1.580 CPU sec, 1.577 real sec... [M::mem_process_seqs] Processed 685168 reads in 160.158 CPU sec, 5.093 real sec [M::mem_process_seqs] Processed 689674 reads in 110.055 CPU sec, 3.500 real sec sorting block 47, #reads = 1067969 mem = 536880067 peak = 871153664 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 280838, 19, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 217, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (267.23, 201.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1198) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 290, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (309.53, 151.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1072) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (330, 394, 1091) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2613) [M::mem_pestat] mean and std.dev: (648.58, 504.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3374) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (122, 310, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022) [M::mem_pestat] mean and std.dev: (259.24, 133.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1322) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688066 sequences (100000046 bp) in 2.614 CPU sec, 2.828 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 280679, 14, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 219, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1172) [M::mem_pestat] mean and std.dev: (245.00, 132.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 293, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (312.18, 151.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1079) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (760, 1079, 1079) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 1717) [M::mem_pestat] mean and std.dev: (827.36, 206.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2036) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 224, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787) [M::mem_pestat] mean and std.dev: (260.83, 125.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 992) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689728 reads in 155.893 CPU sec, 4.957 real sec [M::process] read 687864 sequences (100000167 bp) in 1.590 CPU sec, 1.590 real sec... [M::mem_process_seqs] Processed 688716 reads in 100.870 CPU sec, 3.196 real sec sorting block 48, #reads = 1072534 mem = 536878078 peak = 871153664 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 280803, 5, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 293, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (287.98, 118.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 295, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (314.42, 152.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1084) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 282, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (286.03, 126.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687588 sequences (100000199 bp) in 2.831 CPU sec, 2.955 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 280534, 5, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 248, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (33, 478) [M::mem_pestat] mean and std.dev: (222.57, 69.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 298, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (316.48, 153.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1090) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 228, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (239.75, 92.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1014) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688066 reads in 154.966 CPU sec, 4.916 real sec [M::process] read 687026 sequences (100000117 bp) in 2.042 CPU sec, 2.073 real sec... [M::mem_process_seqs] Processed 687864 reads in 102.430 CPU sec, 3.373 real sec sorting block 49, #reads = 1072167 mem = 536879846 peak = 871153664 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 280052, 18, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 223, 829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2173) [M::mem_pestat] mean and std.dev: (405.06, 428.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2845) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 298, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (316.38, 153.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1086) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (301, 2503, 3009) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8425) [M::mem_pestat] mean and std.dev: (1593.22, 1274.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11133) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 233, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1197) [M::mem_pestat] mean and std.dev: (261.54, 139.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1541) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 50, #reads = 1072118 mem = 536878906 peak = 871153664 [M::process] read 687094 sequences (100000092 bp) in 2.505 CPU sec, 2.631 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 280170, 8, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 207, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974) [M::mem_pestat] mean and std.dev: (291.15, 231.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1250) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 300, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (318.12, 153.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1092) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 220, 662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1652) [M::mem_pestat] mean and std.dev: (359.05, 271.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2147) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687588 reads in 152.850 CPU sec, 4.988 real sec [M::process] read 686740 sequences (100000185 bp) in 1.947 CPU sec, 1.956 real sec... [M::mem_process_seqs] Processed 687026 reads in 100.815 CPU sec, 3.180 real sec sorting block 51, #reads = 1072107 mem = 536878353 peak = 871153664 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 280724, 8, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 213, 616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1488) [M::mem_pestat] mean and std.dev: (354.47, 315.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1924) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 302, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (319.59, 154.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1096) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 235, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074) [M::mem_pestat] mean and std.dev: (343.13, 202.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1362) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686700 sequences (100000256 bp) in 2.652 CPU sec, 2.783 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 279905, 26, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 240, 576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1344) [M::mem_pestat] mean and std.dev: (308.32, 186.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1728) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 304, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (321.74, 155.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (439, 1695, 1695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4207) [M::mem_pestat] mean and std.dev: (1346.65, 962.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5463) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 296, 584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1340) [M::mem_pestat] mean and std.dev: (369.69, 207.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1718) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686642 sequences (100000090 bp) in 1.860 CPU sec, 1.860 real sec... [M::mem_process_seqs] Processed 687094 reads in 160.257 CPU sec, 5.257 real sec [M::mem_process_seqs] Processed 686740 reads in 104.350 CPU sec, 3.313 real sec sorting block 52, #reads = 1070710 mem = 536876451 peak = 871153664 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 279676, 3, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (252, 303, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (279.65, 79.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 304, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (321.09, 154.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 225, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (218.38, 72.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686442 sequences (100000257 bp) in 2.144 CPU sec, 2.297 real sec... [M::process] read 686234 sequences (100000190 bp) in 1.410 CPU sec, 1.408 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 280372, 10, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 255, 564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1352) [M::mem_pestat] mean and std.dev: (383.52, 306.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 305, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (323.63, 156.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (524, 827, 1395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3137) [M::mem_pestat] mean and std.dev: (949.70, 566.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4008) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 300, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (325.71, 192.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686700 reads in 154.065 CPU sec, 4.913 real sec sorting block 53, #reads = 1070161 mem = 536882190 peak = 871153664 [M::mem_process_seqs] Processed 686642 reads in 106.861 CPU sec, 3.416 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 279901, 10, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 218, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988) [M::mem_pestat] mean and std.dev: (244.78, 154.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1258) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 307, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (325.54, 158.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (270, 400, 1415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3705) [M::mem_pestat] mean and std.dev: (784.30, 740.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4850) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 226, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (277.15, 173.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685804 sequences (100000034 bp) in 2.292 CPU sec, 2.427 real sec... [M::process] read 685366 sequences (100000175 bp) in 1.410 CPU sec, 1.415 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 279902, 6, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 247, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (240.35, 82.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 308, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (327.22, 159.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (252, 430, 599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1293) [M::mem_pestat] mean and std.dev: (379.62, 189.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1640) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686442 reads in 153.249 CPU sec, 4.876 real sec sorting block 54, #reads = 1071241 mem = 536879918 peak = 871153664 [M::mem_process_seqs] Processed 686234 reads in 99.339 CPU sec, 3.156 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 279734, 7, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 350, 650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1614) [M::mem_pestat] mean and std.dev: (453.82, 379.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2096) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 310, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (328.21, 158.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 360, 2481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6981) [M::mem_pestat] mean and std.dev: (1387.22, 1896.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9231) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 55, #reads = 1070716 mem = 536877708 peak = 871153664 [M::process] read 685432 sequences (100000261 bp) in 2.860 CPU sec, 2.963 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 279682, 10, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 265, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (237.87, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 311, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (328.09, 157.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (288, 609, 655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1389) [M::mem_pestat] mean and std.dev: (441.00, 233.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1756) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 234, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (228.70, 135.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685804 reads in 155.234 CPU sec, 4.925 real sec [M::process] read 685074 sequences (100000197 bp) in 1.833 CPU sec, 1.861 real sec... [M::mem_process_seqs] Processed 685366 reads in 101.232 CPU sec, 3.349 real sec sorting block 56, #reads = 1070300 mem = 536878867 peak = 871153664 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 279543, 17, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 280, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (276.66, 124.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 313, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (329.97, 159.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (121, 254, 1647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4699) [M::mem_pestat] mean and std.dev: (895.71, 1264.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6225) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 296, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (287.11, 119.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685196 sequences (100000170 bp) in 2.136 CPU sec, 2.282 real sec... [M::process] read 685080 sequences (100000299 bp) in 1.560 CPU sec, 1.564 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 280197, 12, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 249, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (218.12, 80.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 311, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (328.82, 157.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (57, 811, 3275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9711) [M::mem_pestat] mean and std.dev: (1426.50, 1686.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12929) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 359, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012) [M::mem_pestat] mean and std.dev: (301.58, 127.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1278) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685432 reads in 162.735 CPU sec, 5.350 real sec [M::mem_process_seqs] Processed 685074 reads in 106.664 CPU sec, 3.400 real sec sorting block 57, #reads = 1071180 mem = 536879951 peak = 871153664 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 279954, 7, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 666) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1750) [M::mem_pestat] mean and std.dev: (296.82, 251.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2292) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 312, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (328.96, 157.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 329, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (281.00, 81.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684794 sequences (100000196 bp) in 2.581 CPU sec, 2.690 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 279599, 5, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 255, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (266.72, 159.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1093) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 312, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (329.67, 158.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 311, 619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1401) [M::mem_pestat] mean and std.dev: (329.69, 234.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1792) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684820 sequences (100000011 bp) in 1.510 CPU sec, 1.507 real sec... [M::mem_process_seqs] Processed 685196 reads in 157.669 CPU sec, 5.003 real sec sorting block 58, #reads = 1070282 mem = 536878259 peak = 871153664 [M::mem_process_seqs] Processed 685080 reads in 103.348 CPU sec, 3.272 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 278975, 7, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 306, 796) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2024) [M::mem_pestat] mean and std.dev: (393.64, 386.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 312, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (330.18, 159.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 281, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949) [M::mem_pestat] mean and std.dev: (301.43, 167.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1212) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 59, #reads = 1069826 mem = 536880088 peak = 871153664 [M::process] read 685014 sequences (100000077 bp) in 2.370 CPU sec, 2.500 real sec... [M::process] read 684876 sequences (100000265 bp) in 1.420 CPU sec, 1.420 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 279482, 10, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 274, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (248.22, 90.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 309, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (326.06, 155.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (117, 1027, 2221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6429) [M::mem_pestat] mean and std.dev: (1244.20, 946.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8533) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 236, 1083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2909) [M::mem_pestat] mean and std.dev: (459.95, 430.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3822) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684794 reads in 160.298 CPU sec, 5.159 real sec [M::mem_process_seqs] Processed 684820 reads in 101.889 CPU sec, 3.231 real sec sorting block 60, #reads = 1070056 mem = 536880075 peak = 871153664 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 279373, 2, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 259, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1201) [M::mem_pestat] mean and std.dev: (341.77, 263.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 309, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (327.25, 157.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 291, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (269.55, 122.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685106 sequences (100000003 bp) in 2.149 CPU sec, 2.294 real sec... [M::process] read 685184 sequences (100000193 bp) in 1.410 CPU sec, 1.406 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 279199, 6, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 272, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947) [M::mem_pestat] mean and std.dev: (261.58, 125.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1208) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 308, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (326.80, 157.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 269, 735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1881) [M::mem_pestat] mean and std.dev: (267.15, 175.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2454) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685014 reads in 153.001 CPU sec, 4.880 real sec [M::mem_process_seqs] Processed 684876 reads in 107.640 CPU sec, 3.452 real sec sorting block 61, #reads = 1069617 mem = 536879559 peak = 871153664 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 279509, 11, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 254, 758) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1826) [M::mem_pestat] mean and std.dev: (473.44, 405.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 307, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (326.11, 157.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (694, 2439, 3974) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10534) [M::mem_pestat] mean and std.dev: (2117.55, 1577.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13814) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 292, 615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1501) [M::mem_pestat] mean and std.dev: (322.06, 255.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1944) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685692 sequences (100000041 bp) in 3.023 CPU sec, 3.217 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 279607, 3, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 255, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1226) [M::mem_pestat] mean and std.dev: (288.76, 158.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 306, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (325.13, 156.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 287, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016) [M::mem_pestat] mean and std.dev: (302.32, 138.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1292) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685106 reads in 154.167 CPU sec, 4.901 real sec [M::mem_process_seqs] Processed 685184 reads in 102.447 CPU sec, 3.227 real sec sorting block 62, #reads = 1069387 mem = 536880471 peak = 871153664 [M::process] read 685460 sequences (100000047 bp) in 2.167 CPU sec, 2.277 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 279666, 7, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 388, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (332.79, 164.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 304, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (323.57, 156.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 272, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (301.76, 176.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685450 sequences (100000106 bp) in 2.241 CPU sec, 2.395 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 279046, 9, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 183, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (239.09, 139.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 304, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (323.67, 156.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1104) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 307, 654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1634) [M::mem_pestat] mean and std.dev: (390.69, 342.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2124) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685372 sequences (100000247 bp) in 1.410 CPU sec, 1.406 real sec... [M::mem_process_seqs] Processed 685692 reads in 155.445 CPU sec, 4.949 real sec sorting block 63, #reads = 1068928 mem = 536879265 peak = 871153664 [M::mem_process_seqs] Processed 685460 reads in 100.968 CPU sec, 3.200 real sec sorting block 64, #reads = 1070178 mem = 536879027 peak = 889511936 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 279514, 6, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 252, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1159) [M::mem_pestat] mean and std.dev: (257.70, 135.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 305, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (323.11, 155.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1100) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 214, 877) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2313) [M::mem_pestat] mean and std.dev: (249.60, 179.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3031) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685406 sequences (100000080 bp) in 2.190 CPU sec, 2.337 real sec... [M::process] read 685326 sequences (100000286 bp) in 1.410 CPU sec, 1.413 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 279484, 15, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 394, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1160) [M::mem_pestat] mean and std.dev: (325.61, 160.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 304, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (322.58, 154.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1096) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (376, 605, 805) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1663) [M::mem_pestat] mean and std.dev: (633.07, 335.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2092) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 288, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (267.12, 93.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685450 reads in 157.667 CPU sec, 5.213 real sec [M::mem_process_seqs] Processed 685372 reads in 99.936 CPU sec, 3.177 real sec sorting block 65, #reads = 1068407 mem = 536876714 peak = 889511936 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 279454, 5, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 190, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1071) [M::mem_pestat] mean and std.dev: (267.13, 176.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1383) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 304, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (323.84, 156.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1104) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 308, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (290.73, 128.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 989) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685114 sequences (100000127 bp) in 2.175 CPU sec, 2.304 real sec... [M::process] read 685290 sequences (100000129 bp) in 1.420 CPU sec, 1.421 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 279823, 8, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 256, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (254.80, 102.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 303, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (321.42, 154.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1091) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (366, 397, 594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050) [M::mem_pestat] mean and std.dev: (404.69, 141.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1278) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685406 reads in 155.095 CPU sec, 5.011 real sec [M::mem_process_seqs] Processed 685326 reads in 96.911 CPU sec, 3.078 real sec sorting block 66, #reads = 1068622 mem = 536883123 peak = 889511936 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 279315, 8, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 311, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1172) [M::mem_pestat] mean and std.dev: (292.33, 176.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 305, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (324.73, 155.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 285, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (279.93, 144.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1176) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685010 sequences (100000122 bp) in 1.987 CPU sec, 2.113 real sec... [M::process] read 685206 sequences (100000140 bp) in 1.410 CPU sec, 1.411 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 279538, 13, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 225, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1263) [M::mem_pestat] mean and std.dev: (353.35, 327.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 307, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (326.41, 157.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (365, 365, 1542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3896) [M::mem_pestat] mean and std.dev: (788.46, 714.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5073) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 291, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (304.46, 117.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1079) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685114 reads in 148.260 CPU sec, 4.716 real sec sorting block 67, #reads = 1068909 mem = 536876678 peak = 889511936 [M::mem_process_seqs] Processed 685290 reads in 96.672 CPU sec, 3.074 real sec sorting block 68, #reads = 1068813 mem = 536881976 peak = 889511936 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 279600, 13, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 316, 565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1301) [M::mem_pestat] mean and std.dev: (319.92, 163.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 309, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (328.00, 158.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (241, 463, 1698) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4612) [M::mem_pestat] mean and std.dev: (635.75, 524.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6069) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 234, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (225.80, 83.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684856 sequences (100000035 bp) in 2.276 CPU sec, 2.430 real sec... [M::process] read 684412 sequences (100000013 bp) in 1.430 CPU sec, 1.426 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 279510, 11, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 260, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (297.50, 97.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 311, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (330.39, 161.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1142) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (362, 362, 974) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2198) [M::mem_pestat] mean and std.dev: (583.10, 483.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2810) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 216, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (235.24, 96.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685010 reads in 148.065 CPU sec, 4.706 real sec [M::mem_process_seqs] Processed 685206 reads in 96.448 CPU sec, 3.280 real sec sorting block 69, #reads = 1069025 mem = 536879888 peak = 889511936 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278995, 7, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 262, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (245.69, 140.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 309, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (329.05, 159.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 251, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (281.24, 131.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685808 sequences (100000053 bp) in 2.088 CPU sec, 2.212 real sec... [M::process] read 689844 sequences (100000152 bp) in 1.410 CPU sec, 1.412 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 279326, 3, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 303, 968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2528) [M::mem_pestat] mean and std.dev: (560.97, 712.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3411) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 315, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (334.81, 163.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1164) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 525, 790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1918) [M::mem_pestat] mean and std.dev: (462.21, 308.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2482) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684856 reads in 154.793 CPU sec, 5.136 real sec [M::mem_process_seqs] Processed 684412 reads in 98.112 CPU sec, 3.119 real sec sorting block 70, #reads = 1068583 mem = 536879016 peak = 889511936 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 280146, 18, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 270, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (245.81, 116.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 309, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (329.92, 162.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1155) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (379, 879, 951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2095) [M::mem_pestat] mean and std.dev: (753.94, 471.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2667) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 269, 567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1329) [M::mem_pestat] mean and std.dev: (419.62, 359.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1857) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689164 sequences (100000186 bp) in 2.206 CPU sec, 2.344 real sec... [M::process] read 688264 sequences (100000131 bp) in 1.390 CPU sec, 1.389 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 280979, 11, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 318, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797) [M::mem_pestat] mean and std.dev: (269.21, 118.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1005) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 288, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (309.24, 152.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1075) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (813, 1609, 1857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3945) [M::mem_pestat] mean and std.dev: (1275.73, 613.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4989) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 249, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (277.32, 128.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685808 reads in 154.706 CPU sec, 4.924 real sec sorting block 71, #reads = 1069009 mem = 536880160 peak = 889511936 [M::mem_process_seqs] Processed 689844 reads in 99.741 CPU sec, 3.256 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 280356, 9, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 191, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (252.91, 188.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1208) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 293, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (313.74, 155.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1090) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 261, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060) [M::mem_pestat] mean and std.dev: (295.38, 153.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1353) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687928 sequences (100000187 bp) in 2.013 CPU sec, 2.149 real sec... [M::process] read 687278 sequences (100000157 bp) in 1.360 CPU sec, 1.365 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 280584, 6, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 300, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1058) [M::mem_pestat] mean and std.dev: (335.60, 234.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1362) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 296, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (315.95, 154.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1092) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 377, 579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1281) [M::mem_pestat] mean and std.dev: (383.79, 200.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1632) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689164 reads in 153.118 CPU sec, 4.971 real sec sorting block 72, #reads = 1070620 mem = 536879341 peak = 889511936 [M::mem_process_seqs] Processed 688264 reads in 97.692 CPU sec, 3.130 real sec sorting block 73, #reads = 1072317 mem = 536877592 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 280358, 11, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 177, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (201.12, 105.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 301, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (321.31, 159.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (701, 1000, 1676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3626) [M::mem_pestat] mean and std.dev: (991.80, 614.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4601) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 307, 811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2043) [M::mem_pestat] mean and std.dev: (483.22, 417.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2659) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687352 sequences (100000013 bp) in 2.067 CPU sec, 2.207 real sec... [M::process] read 686550 sequences (100000090 bp) in 1.360 CPU sec, 1.366 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 280091, 2, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 273, 615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1537) [M::mem_pestat] mean and std.dev: (381.12, 281.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1998) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 301, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (321.04, 157.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 281, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (267.74, 72.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687928 reads in 146.587 CPU sec, 4.671 real sec [M::mem_process_seqs] Processed 687278 reads in 101.040 CPU sec, 3.225 real sec sorting block 74, #reads = 1073275 mem = 536880535 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 280155, 12, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 264, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (280.15, 139.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 301, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (320.93, 157.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (753, 852, 1460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2874) [M::mem_pestat] mean and std.dev: (1000.08, 599.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3581) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 377, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973) [M::mem_pestat] mean and std.dev: (338.05, 181.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1231) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686592 sequences (100000107 bp) in 2.184 CPU sec, 2.340 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 279985, 9, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 272, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1280) [M::mem_pestat] mean and std.dev: (251.08, 162.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 302, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (320.46, 155.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1097) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 220, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957) [M::mem_pestat] mean and std.dev: (274.72, 154.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1214) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686458 sequences (100000004 bp) in 1.420 CPU sec, 1.418 real sec... [M::mem_process_seqs] Processed 687352 reads in 145.260 CPU sec, 4.621 real sec [M::mem_process_seqs] Processed 686550 reads in 94.932 CPU sec, 3.015 real sec sorting block 75, #reads = 1072196 mem = 536880388 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 279734, 9, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 207, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (255.06, 138.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 305, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (323.17, 156.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 289, 857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2141) [M::mem_pestat] mean and std.dev: (362.93, 246.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2783) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686436 sequences (100000104 bp) in 2.644 CPU sec, 2.824 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 279977, 15, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 225, 838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2180) [M::mem_pestat] mean and std.dev: (392.44, 418.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2851) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 306, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (324.89, 158.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (357, 736, 1579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4023) [M::mem_pestat] mean and std.dev: (774.00, 471.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5245) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (263, 499, 886) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2132) [M::mem_pestat] mean and std.dev: (547.60, 426.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2755) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686220 sequences (100000140 bp) in 1.530 CPU sec, 1.528 real sec... [M::mem_process_seqs] Processed 686592 reads in 146.916 CPU sec, 4.667 real sec sorting block 76, #reads = 1072735 mem = 536877107 peak = 936890368 [M::mem_process_seqs] Processed 686458 reads in 96.702 CPU sec, 3.074 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 279958, 13, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 260, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (275.49, 142.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 309, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (327.38, 159.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (537, 537, 1947) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4767) [M::mem_pestat] mean and std.dev: (1189.15, 1165.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6177) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 266, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (313.33, 185.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1188) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 77, #reads = 1071798 mem = 536880198 peak = 936890368 [M::process] read 685834 sequences (100000210 bp) in 2.273 CPU sec, 2.420 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 279946, 6, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 203, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (236.00, 130.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 309, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (327.73, 159.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (124, 163, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (191.95, 112.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685892 sequences (100000008 bp) in 1.430 CPU sec, 1.435 real sec... [M::mem_process_seqs] Processed 686436 reads in 146.056 CPU sec, 4.644 real sec [M::mem_process_seqs] Processed 686220 reads in 95.076 CPU sec, 3.012 real sec sorting block 78, #reads = 1071676 mem = 536883746 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 279770, 15, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (269, 351, 1027) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2543) [M::mem_pestat] mean and std.dev: (521.03, 415.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3301) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 310, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (329.31, 160.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (620, 2047, 2047) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4901) [M::mem_pestat] mean and std.dev: (1328.71, 840.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6328) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 283, 749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1843) [M::mem_pestat] mean and std.dev: (465.04, 449.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2390) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685554 sequences (100000081 bp) in 2.472 CPU sec, 2.657 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 279500, 12, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 291, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (210.41, 86.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 311, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (329.53, 160.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (664, 828, 1653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3631) [M::mem_pestat] mean and std.dev: (1176.25, 991.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5142) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 244, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018) [M::mem_pestat] mean and std.dev: (314.51, 185.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1287) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685398 sequences (100000007 bp) in 1.470 CPU sec, 1.468 real sec... [M::mem_process_seqs] Processed 685834 reads in 146.909 CPU sec, 4.670 real sec [M::mem_process_seqs] Processed 685892 reads in 95.836 CPU sec, 3.036 real sec sorting block 79, #reads = 1071061 mem = 536877854 peak = 936890368 [M::process] read 685398 sequences (100000159 bp) in 2.158 CPU sec, 2.316 real sec... [M::process] read 685604 sequences (100000053 bp) in 1.450 CPU sec, 1.454 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 279622, 4, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 182, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (185.60, 74.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 312, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (330.10, 159.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1138) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 289, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (291.29, 139.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685398 reads in 86.593 CPU sec, 2.751 real sec sorting block 80, #reads = 1071734 mem = 536877737 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 279681, 8, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 238, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1243) [M::mem_pestat] mean and std.dev: (286.26, 214.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 313, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (332.62, 162.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1162) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 203, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (218.91, 100.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 279687, 7, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 242, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (223.73, 72.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 312, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (330.26, 159.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1142) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 206, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (206.40, 59.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685554 reads in 230.145 CPU sec, 7.394 real sec [M::mem_process_seqs] Processed 685398 reads in 113.859 CPU sec, 3.635 real sec [M::process] read 685242 sequences (100000033 bp) in 3.119 CPU sec, 3.271 real sec... sorting block 81, #reads = 1071936 mem = 536882754 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 279669, 7, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 272, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (257.32, 102.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1016) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 311, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (329.21, 159.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (126, 286, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993) [M::mem_pestat] mean and std.dev: (266.68, 130.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1282) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 279698, 8, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 231, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (236.74, 112.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 311, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (329.62, 159.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 276, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (258.71, 109.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1177) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685604 reads in 155.836 CPU sec, 5.018 real sec [M::process] read 684920 sequences (100000129 bp) in 3.363 CPU sec, 3.833 real sec... [M::mem_process_seqs] Processed 685242 reads in 104.037 CPU sec, 3.349 real sec sorting block 82, #reads = 1071063 mem = 536886883 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 279044, 6, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 411, 1151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3181) [M::mem_pestat] mean and std.dev: (665.29, 704.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4196) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 310, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (328.70, 158.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 527, 1531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4119) [M::mem_pestat] mean and std.dev: (527.00, 422.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5413) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684920 reads in 86.911 CPU sec, 2.907 real sec [M::process] read 684146 sequences (100000086 bp) in 3.777 CPU sec, 4.155 real sec... sorting block 83, #reads = 1070392 mem = 536876919 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 279202, 11, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 228, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1181) [M::mem_pestat] mean and std.dev: (288.85, 215.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 684266 sequences (100000173 bp) in 2.193 CPU sec, 2.416 real sec... [M::mem_pestat] (25, 50, 75) percentile: (205, 313, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (330.07, 157.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (345, 1375, 3202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8916) [M::mem_pestat] mean and std.dev: (2207.91, 2003.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11773) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 244, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (212.82, 64.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685726 sequences (100000147 bp) in 1.413 CPU sec, 1.421 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 278621, 6, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 161, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (179.36, 73.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 315, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (332.80, 159.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 236, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1171) [M::mem_pestat] mean and std.dev: (324.87, 180.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1490) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684146 reads in 152.623 CPU sec, 4.999 real sec [M::process] read 685894 sequences (100000121 bp) in 1.482 CPU sec, 1.519 real sec... sorting block 84, #reads = 1069501 mem = 536878846 peak = 936890368 [M::process] read 685734 sequences (100000109 bp) in 1.628 CPU sec, 1.638 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 279141, 10, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 236, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (256.27, 151.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1202) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 305, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (324.19, 157.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 227, 644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1872) [M::mem_pestat] mean and std.dev: (346.89, 454.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2486) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 245, 593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1445) [M::mem_pestat] mean and std.dev: (338.45, 274.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1871) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685726 reads in 83.057 CPU sec, 2.814 real sec [M::mem_process_seqs] Processed 684266 reads in 201.347 CPU sec, 6.567 real sec sorting block 85, #reads = 1069049 mem = 536878519 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 279490, 12, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 260, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (243.66, 121.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 301, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (320.60, 154.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 568, 1516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4452) [M::mem_pestat] mean and std.dev: (915.83, 1031.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5920) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 307, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (267.25, 135.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685716 sequences (100000267 bp) in 2.586 CPU sec, 2.823 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 279878, 11, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (244, 274, 1362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3598) [M::mem_pestat] mean and std.dev: (770.89, 918.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 302, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (320.21, 153.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (515, 546, 3255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8735) [M::mem_pestat] mean and std.dev: (1018.20, 1142.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11475) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 258, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (297.94, 174.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685894 reads in 164.758 CPU sec, 5.273 real sec [M::mem_process_seqs] Processed 685734 reads in 97.648 CPU sec, 3.087 real sec [M::process] read 685760 sequences (100000134 bp) in 1.786 CPU sec, 1.855 real sec... sorting block 86, #reads = 1068435 mem = 536877306 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 279773, 7, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 363, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (305.31, 161.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 303, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (322.45, 155.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 208, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (235.09, 108.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685408 sequences (100000103 bp) in 3.132 CPU sec, 3.366 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 278817, 7, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 217, 663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1711) [M::mem_pestat] mean and std.dev: (346.22, 328.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2235) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 303, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (322.06, 155.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 373, 764) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1818) [M::mem_pestat] mean and std.dev: (486.81, 324.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2345) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685716 reads in 150.093 CPU sec, 4.790 real sec [M::mem_process_seqs] Processed 685760 reads in 99.017 CPU sec, 3.128 real sec sorting block 87, #reads = 1069445 mem = 536879821 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 279150, 15, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 240, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (229.19, 90.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 303, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (322.55, 155.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (371, 546, 2144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5690) [M::mem_pestat] mean and std.dev: (1058.33, 1048.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7463) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 256, 898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2368) [M::mem_pestat] mean and std.dev: (483.41, 446.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3103) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685408 reads in 83.359 CPU sec, 2.759 real sec [M::process] read 685286 sequences (100000047 bp) in 3.711 CPU sec, 4.021 real sec... sorting block 88, #reads = 1069240 mem = 536880970 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 279331, 11, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 184, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (163.44, 42.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 303, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (321.53, 153.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1091) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (346, 346, 1447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3649) [M::mem_pestat] mean and std.dev: (699.30, 546.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4750) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 281, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955) [M::mem_pestat] mean and std.dev: (292.60, 162.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1203) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685558 sequences (100000076 bp) in 2.464 CPU sec, 2.697 real sec... [M::mem_process_seqs] Processed 685286 reads in 82.889 CPU sec, 2.735 real sec sorting block 89, #reads = 1069542 mem = 536878980 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 279709, 3, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 319, 790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1918) [M::mem_pestat] mean and std.dev: (478.38, 364.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 302, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (320.84, 153.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1091) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 241, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037) [M::mem_pestat] mean and std.dev: (277.21, 182.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1325) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685548 sequences (100000164 bp) in 2.448 CPU sec, 2.725 real sec... [M::mem_process_seqs] Processed 685558 reads in 84.132 CPU sec, 2.854 real sec sorting block 90, #reads = 1069998 mem = 536880857 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 279029, 9, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 237, 583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1387) [M::mem_pestat] mean and std.dev: (313.47, 178.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 302, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (321.32, 154.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 246, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 511) [M::mem_pestat] mean and std.dev: (227.10, 69.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685494 sequences (100000253 bp) in 2.141 CPU sec, 2.309 real sec... [M::mem_process_seqs] Processed 685548 reads in 82.844 CPU sec, 2.675 real sec [M::process] read 685158 sequences (100000203 bp) in 2.147 CPU sec, 2.306 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 279741, 6, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 296, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (273.77, 116.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 305, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (324.06, 155.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 310, 593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1429) [M::mem_pestat] mean and std.dev: (368.65, 306.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1847) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685612 sequences (100000091 bp) in 1.368 CPU sec, 1.376 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 279530, 13, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 246, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001) [M::mem_pestat] mean and std.dev: (280.48, 144.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1271) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 307, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (325.36, 156.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (261, 394, 1698) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4572) [M::mem_pestat] mean and std.dev: (719.00, 597.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6009) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 277, 616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1528) [M::mem_pestat] mean and std.dev: (370.14, 325.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1984) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684854 sequences (100000298 bp) in 1.270 CPU sec, 1.269 real sec... [M::mem_process_seqs] Processed 685494 reads in 146.003 CPU sec, 4.597 real sec sorting block 91, #reads = 1068706 mem = 536880962 peak = 936890368 [M::mem_process_seqs] Processed 685158 reads in 102.108 CPU sec, 3.242 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 278648, 4, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 172, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (199.88, 103.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 305, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (323.67, 155.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1104) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 313, 665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1627) [M::mem_pestat] mean and std.dev: (401.00, 270.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2108) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684270 sequences (100000246 bp) in 2.445 CPU sec, 2.713 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 279110, 5, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 231, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (283.35, 141.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 309, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (328.01, 158.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 251, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (251.03, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685612 reads in 146.673 CPU sec, 4.654 real sec sorting block 92, #reads = 1069591 mem = 536877578 peak = 936890368 [M::mem_process_seqs] Processed 684854 reads in 96.727 CPU sec, 3.052 real sec [M::process] read 684078 sequences (100000024 bp) in 2.114 CPU sec, 2.196 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278913, 22, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 344, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (317.75, 142.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 314, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (332.75, 160.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (530, 566, 566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (458, 638) [M::mem_pestat] mean and std.dev: (551.57, 20.06) [M::mem_pestat] low and high boundaries for proper pairs: (422, 674) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 297, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060) [M::mem_pestat] mean and std.dev: (320.92, 224.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1360) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 93, #reads = 1067343 mem = 536878200 peak = 936890368 [M::process] read 685148 sequences (100000149 bp) in 3.030 CPU sec, 3.267 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 279269, 20, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 389, 840) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2196) [M::mem_pestat] mean and std.dev: (517.98, 564.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2874) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 316, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (334.74, 161.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (373, 494, 1780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4594) [M::mem_pestat] mean and std.dev: (784.95, 688.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6001) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 311, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (328.09, 122.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684270 reads in 147.838 CPU sec, 4.707 real sec [M::mem_process_seqs] Processed 684078 reads in 98.435 CPU sec, 3.114 real sec [M::process] read 686114 sequences (100000188 bp) in 3.135 CPU sec, 3.383 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 279186, 9, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 260, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (282.97, 132.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 309, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (328.94, 159.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 335, 683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1605) [M::mem_pestat] mean and std.dev: (351.29, 169.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2066) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 94, #reads = 1069622 mem = 536879545 peak = 936890368 [M::process] read 696614 sequences (100000242 bp) in 2.013 CPU sec, 2.154 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 279907, 7, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 228, 776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1962) [M::mem_pestat] mean and std.dev: (414.95, 450.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 307, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (327.96, 161.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1150) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 179, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (219.79, 105.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685148 reads in 152.728 CPU sec, 4.891 real sec [M::mem_process_seqs] Processed 686114 reads in 102.515 CPU sec, 3.245 real sec sorting block 95, #reads = 1069248 mem = 536882860 peak = 936890368 [M::process] read 693468 sequences (100000048 bp) in 1.835 CPU sec, 1.931 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 283696, 7, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 244, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (248.69, 117.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 258, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (286.74, 147.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1017) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 268, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (268.53, 112.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692826 sequences (100000155 bp) in 2.132 CPU sec, 2.267 real sec... [M::process] read 691760 sequences (100000272 bp) in 1.360 CPU sec, 1.363 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282722, 10, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 283, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (296.00, 144.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 271, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845) [M::mem_pestat] mean and std.dev: (298.71, 152.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1068) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (412, 902, 1008) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2200) [M::mem_pestat] mean and std.dev: (721.56, 266.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2796) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (299, 375, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833) [M::mem_pestat] mean and std.dev: (321.56, 111.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696614 reads in 167.647 CPU sec, 5.342 real sec sorting block 96, #reads = 1068091 mem = 536881519 peak = 936890368 [M::mem_process_seqs] Processed 693468 reads in 108.259 CPU sec, 3.463 real sec sorting block 97, #reads = 1071587 mem = 536882310 peak = 936890368 [M::process] read 691368 sequences (100000025 bp) in 2.031 CPU sec, 2.168 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282216, 13, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 340, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1186) [M::mem_pestat] mean and std.dev: (324.17, 205.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 277, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (302.88, 153.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1087) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (331, 795, 1612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4174) [M::mem_pestat] mean and std.dev: (666.82, 425.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5455) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 315, 2484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7046) [M::mem_pestat] mean and std.dev: (1516.70, 1837.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9327) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 690814 sequences (100000223 bp) in 1.410 CPU sec, 1.403 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 282501, 6, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 333, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (315.33, 153.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1151) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 281, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (305.40, 153.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1093) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 193, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (231.72, 88.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692826 reads in 166.395 CPU sec, 5.483 real sec [M::mem_process_seqs] Processed 691760 reads in 104.452 CPU sec, 3.341 real sec sorting block 98, #reads = 1070164 mem = 536875426 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 282825, 5, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 274, 1124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3008) [M::mem_pestat] mean and std.dev: (431.38, 424.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3950) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 284, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (308.38, 155.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 258, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (252.19, 76.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688996 sequences (100000095 bp) in 1.939 CPU sec, 2.041 real sec... [M::process] read 689340 sequences (100000107 bp) in 1.410 CPU sec, 1.411 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 281985, 8, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 370, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1162) [M::mem_pestat] mean and std.dev: (369.05, 187.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 286, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (310.53, 156.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 290, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1139) [M::mem_pestat] mean and std.dev: (298.75, 137.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1457) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691368 reads in 161.421 CPU sec, 5.153 real sec [M::mem_process_seqs] Processed 690814 reads in 106.182 CPU sec, 3.409 real sec sorting block 99, #reads = 1071396 mem = 536878565 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 281637, 1, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 266, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1174) [M::mem_pestat] mean and std.dev: (264.08, 127.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 292, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (313.86, 154.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 293, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (271.28, 95.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688456 sequences (100000193 bp) in 2.254 CPU sec, 2.389 real sec... [M::process] read 687922 sequences (100000019 bp) in 1.599 CPU sec, 1.608 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 281739, 18, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 254, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (225.97, 93.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 294, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (317.59, 158.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (224, 1192, 1192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3128) [M::mem_pestat] mean and std.dev: (861.39, 607.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4096) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 246, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (261.18, 110.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688996 reads in 164.057 CPU sec, 5.237 real sec [M::mem_process_seqs] Processed 689340 reads in 111.579 CPU sec, 3.575 real sec sorting block 100, #reads = 1070316 mem = 536881137 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 280973, 11, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 343, 889) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2295) [M::mem_pestat] mean and std.dev: (590.58, 575.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2998) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 298, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (320.81, 160.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1150) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (796, 1050, 1996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4396) [M::mem_pestat] mean and std.dev: (909.56, 481.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5596) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 286, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (255.50, 85.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 101, #reads = 1070080 mem = 536881900 peak = 936890368 [M::process] read 687938 sequences (100000213 bp) in 2.549 CPU sec, 2.739 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 280591, 6, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 230, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (199.96, 75.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 302, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (323.51, 160.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 229, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (268.89, 127.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688456 reads in 167.710 CPU sec, 5.379 real sec [M::process] read 687714 sequences (100000067 bp) in 1.722 CPU sec, 1.740 real sec... [M::mem_process_seqs] Processed 687922 reads in 109.702 CPU sec, 3.512 real sec sorting block 102, #reads = 1069244 mem = 536877226 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 280890, 6, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (311, 365, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (13, 758) [M::mem_pestat] mean and std.dev: (326.75, 120.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 303, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (324.59, 161.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1165) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 349, 499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1049) [M::mem_pestat] mean and std.dev: (372.80, 192.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1324) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688026 sequences (100000164 bp) in 2.522 CPU sec, 2.703 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 281178, 8, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 289, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (250.34, 88.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 303, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (324.29, 160.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 263, 580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1404) [M::mem_pestat] mean and std.dev: (323.31, 197.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1816) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688168 sequences (100000090 bp) in 1.610 CPU sec, 1.611 real sec... [M::mem_process_seqs] Processed 687938 reads in 165.655 CPU sec, 5.309 real sec [M::mem_process_seqs] Processed 687714 reads in 109.209 CPU sec, 3.481 real sec sorting block 103, #reads = 1070565 mem = 536879642 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 280974, 16, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 358, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076) [M::mem_pestat] mean and std.dev: (383.45, 195.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1362) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 302, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (322.43, 159.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (250, 475, 920) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2260) [M::mem_pestat] mean and std.dev: (632.00, 477.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2930) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 322, 719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1817) [M::mem_pestat] mean and std.dev: (331.55, 252.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2366) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687714 sequences (100000101 bp) in 2.726 CPU sec, 2.924 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 281576, 19, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 265, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (277.38, 97.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 300, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (320.30, 157.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1137, 1137, 1594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (223, 2508) [M::mem_pestat] mean and std.dev: (1303.15, 263.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2965) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 237, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (306.09, 181.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1031) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688026 reads in 171.450 CPU sec, 5.441 real sec [M::process] read 687652 sequences (100000018 bp) in 2.410 CPU sec, 2.428 real sec... [M::mem_process_seqs] Processed 688168 reads in 111.472 CPU sec, 3.531 real sec sorting block 104, #reads = 1068411 mem = 536877379 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 280818, 3, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 243, 1190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3246) [M::mem_pestat] mean and std.dev: (481.21, 473.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4274) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 302, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (322.20, 157.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1142) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (244, 321, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (319.97, 156.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687932 sequences (100000229 bp) in 3.640 CPU sec, 3.866 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 280853, 15, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (271, 368, 2090) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5728) [M::mem_pestat] mean and std.dev: (843.84, 972.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 302, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (322.82, 158.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1185, 4431, 4431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10923) [M::mem_pestat] mean and std.dev: (3630.40, 2089.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14169) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 318, 1203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3139) [M::mem_pestat] mean and std.dev: (645.02, 656.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4107) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687714 reads in 177.062 CPU sec, 5.810 real sec sorting block 105, #reads = 1066466 mem = 536877523 peak = 936890368 [M::mem_process_seqs] Processed 687652 reads in 115.681 CPU sec, 3.664 real sec [M::process] read 687610 sequences (100000137 bp) in 2.136 CPU sec, 2.238 real sec... sorting block 106, #reads = 1066027 mem = 536880888 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 280395, 7, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 253, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1219) [M::mem_pestat] mean and std.dev: (328.96, 237.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 303, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (322.40, 158.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 406, 667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1547) [M::mem_pestat] mean and std.dev: (470.00, 365.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1987) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688308 sequences (100000091 bp) in 2.492 CPU sec, 2.724 real sec... [M::process] read 687662 sequences (100000249 bp) in 1.430 CPU sec, 1.430 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 281003, 11, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 244, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (277.00, 158.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 303, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (321.92, 157.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (908, 1293, 2271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4997) [M::mem_pestat] mean and std.dev: (1632.64, 1090.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6360) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 312, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937) [M::mem_pestat] mean and std.dev: (304.72, 136.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1185) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687932 reads in 172.711 CPU sec, 5.515 real sec [M::mem_process_seqs] Processed 687610 reads in 112.878 CPU sec, 3.615 real sec sorting block 107, #reads = 1067335 mem = 536876671 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 280395, 13, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 254, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1193) [M::mem_pestat] mean and std.dev: (333.73, 256.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 295, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (317.13, 156.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (500, 575, 1238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2714) [M::mem_pestat] mean and std.dev: (669.33, 349.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3452) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 380, 550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1224) [M::mem_pestat] mean and std.dev: (379.63, 228.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1561) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686816 sequences (100000072 bp) in 2.322 CPU sec, 2.516 real sec... [M::process] read 687030 sequences (100000107 bp) in 1.450 CPU sec, 1.455 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 280443, 10, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 269, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (275.12, 133.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 301, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (322.66, 158.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (187, 1385, 1429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3913) [M::mem_pestat] mean and std.dev: (886.56, 625.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5155) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 245, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (288.17, 164.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1078) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688308 reads in 175.730 CPU sec, 5.792 real sec [M::mem_process_seqs] Processed 687662 reads in 111.624 CPU sec, 3.575 real sec sorting block 108, #reads = 1067638 mem = 536880655 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 280157, 24, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 209, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035) [M::mem_pestat] mean and std.dev: (252.79, 135.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1332) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 304, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (323.08, 156.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (165, 684, 2053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5829) [M::mem_pestat] mean and std.dev: (1204.00, 1308.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7717) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (238, 348, 1186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3082) [M::mem_pestat] mean and std.dev: (586.66, 616.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4030) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686872 sequences (100000043 bp) in 2.445 CPU sec, 2.658 real sec... [M::process] read 686094 sequences (100000154 bp) in 1.460 CPU sec, 1.461 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 280584, 16, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 203, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (258.45, 142.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1168) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 304, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (324.29, 158.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (410, 436, 1566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3878) [M::mem_pestat] mean and std.dev: (665.07, 514.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5034) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 311, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1113) [M::mem_pestat] mean and std.dev: (318.35, 148.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1427) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686816 reads in 177.583 CPU sec, 5.755 real sec sorting block 109, #reads = 1067716 mem = 536878401 peak = 936890368 [M::mem_process_seqs] Processed 687030 reads in 113.101 CPU sec, 3.638 real sec sorting block 110, #reads = 1066492 mem = 536880069 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 280131, 12, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (261, 529, 1180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3018) [M::mem_pestat] mean and std.dev: (803.17, 775.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3937) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 304, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (324.40, 158.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (108, 557, 1320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3744) [M::mem_pestat] mean and std.dev: (519.73, 496.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4956) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 312, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (295.10, 124.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686754 sequences (100000095 bp) in 2.256 CPU sec, 2.435 real sec... [M::process] read 686496 sequences (100000169 bp) in 1.450 CPU sec, 1.452 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 279623, 5, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 273, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966) [M::mem_pestat] mean and std.dev: (270.73, 165.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1241) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 307, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (327.44, 160.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (256, 382, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994) [M::mem_pestat] mean and std.dev: (364.67, 134.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1240) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686872 reads in 176.707 CPU sec, 5.679 real sec [M::mem_process_seqs] Processed 686094 reads in 115.439 CPU sec, 3.718 real sec sorting block 111, #reads = 1066121 mem = 536881457 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 280099, 6, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 204, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (242.14, 136.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1078) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 303, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (324.66, 159.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 364, 750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1778) [M::mem_pestat] mean and std.dev: (432.82, 307.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2292) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686358 sequences (100000040 bp) in 2.260 CPU sec, 2.448 real sec... [M::process] read 685736 sequences (100000080 bp) in 1.450 CPU sec, 1.451 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 279971, 13, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 256, 621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1509) [M::mem_pestat] mean and std.dev: (384.93, 286.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1953) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 303, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (324.30, 158.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (488, 691, 1452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3380) [M::mem_pestat] mean and std.dev: (841.00, 614.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4344) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964) [M::mem_pestat] mean and std.dev: (252.00, 174.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1237) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686754 reads in 173.408 CPU sec, 5.537 real sec [M::mem_process_seqs] Processed 686496 reads in 113.638 CPU sec, 3.640 real sec sorting block 112, #reads = 1064606 mem = 536878953 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 279164, 15, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 210, 813) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2165) [M::mem_pestat] mean and std.dev: (390.89, 337.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2841) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 305, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (324.58, 157.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (394, 485, 1253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2971) [M::mem_pestat] mean and std.dev: (727.53, 628.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3830) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 293, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (300.96, 110.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685804 sequences (100000151 bp) in 2.490 CPU sec, 2.664 real sec... [M::process] read 686098 sequences (100000219 bp) in 1.460 CPU sec, 1.462 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 280142, 12, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 361, 1049) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2739) [M::mem_pestat] mean and std.dev: (532.78, 423.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 308, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (327.33, 158.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (538, 606, 1301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2827) [M::mem_pestat] mean and std.dev: (867.50, 503.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3590) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 304, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047) [M::mem_pestat] mean and std.dev: (317.60, 191.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1333) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686358 reads in 172.373 CPU sec, 5.504 real sec sorting block 113, #reads = 1065602 mem = 536882317 peak = 936890368 [M::mem_process_seqs] Processed 685736 reads in 118.931 CPU sec, 3.822 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 279541, 8, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 353, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993) [M::mem_pestat] mean and std.dev: (304.95, 182.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1261) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 308, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (328.49, 160.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1155) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 299, 565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1387) [M::mem_pestat] mean and std.dev: (322.35, 219.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1798) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 114, #reads = 1064864 mem = 536878427 peak = 936890368 [M::process] read 685478 sequences (100000196 bp) in 2.278 CPU sec, 2.457 real sec... [M::process] read 685208 sequences (100000094 bp) in 1.460 CPU sec, 1.461 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 279886, 9, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 206, 552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1354) [M::mem_pestat] mean and std.dev: (351.76, 279.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 307, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (327.03, 159.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1150) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (260, 342, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1064) [M::mem_pestat] mean and std.dev: (323.41, 133.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1332) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685804 reads in 169.912 CPU sec, 5.429 real sec [M::mem_process_seqs] Processed 686098 reads in 112.536 CPU sec, 3.601 real sec sorting block 115, #reads = 1065324 mem = 536878530 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 279730, 12, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 364, 811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2099) [M::mem_pestat] mean and std.dev: (494.55, 435.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 310, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (331.24, 161.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1168) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (536, 648, 9780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 28268) [M::mem_pestat] mean and std.dev: (3493.75, 3923.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 37512) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 333, 549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1203) [M::mem_pestat] mean and std.dev: (345.37, 169.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1530) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685578 sequences (100000188 bp) in 3.132 CPU sec, 3.427 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 279785, 6, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 267, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (297.83, 172.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 312, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (333.67, 163.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1177) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (301, 381, 907) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2119) [M::mem_pestat] mean and std.dev: (472.85, 273.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2725) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 696874 sequences (100000083 bp) in 1.523 CPU sec, 1.533 real sec... [M::mem_process_seqs] Processed 685478 reads in 172.007 CPU sec, 5.476 real sec [M::mem_process_seqs] Processed 685208 reads in 113.776 CPU sec, 3.621 real sec sorting block 116, #reads = 1064796 mem = 536880796 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 279647, 6, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 341, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951) [M::mem_pestat] mean and std.dev: (286.92, 135.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1206) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 311, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923) [M::mem_pestat] mean and std.dev: (331.80, 162.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1164) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 334, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002) [M::mem_pestat] mean and std.dev: (320.21, 136.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1269) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 696704 sequences (100000294 bp) in 2.312 CPU sec, 2.462 real sec... [M::process] read 694632 sequences (100000286 bp) in 1.500 CPU sec, 1.493 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 283654, 14, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 208, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (219.75, 87.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 258, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (285.69, 146.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (652, 1076, 1740) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3916) [M::mem_pestat] mean and std.dev: (987.17, 601.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5004) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 442, 831) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2109) [M::mem_pestat] mean and std.dev: (639.60, 582.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2971) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685578 reads in 183.288 CPU sec, 5.882 real sec [M::mem_process_seqs] Processed 696874 reads in 122.797 CPU sec, 3.920 real sec sorting block 117, #reads = 1064706 mem = 536887195 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 283712, 6, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 230, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (257.56, 119.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 258, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799) [M::mem_pestat] mean and std.dev: (284.76, 144.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1009) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 291, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (248.71, 79.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693402 sequences (100000174 bp) in 2.417 CPU sec, 2.574 real sec... [M::process] read 693356 sequences (100000030 bp) in 1.480 CPU sec, 1.485 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 283089, 12, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 244, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (261.82, 112.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 269, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (292.96, 146.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (192, 418, 843) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2145) [M::mem_pestat] mean and std.dev: (505.73, 466.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2796) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 265, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009) [M::mem_pestat] mean and std.dev: (277.39, 175.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1284) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696704 reads in 183.384 CPU sec, 5.859 real sec sorting block 118, #reads = 1063962 mem = 536879219 peak = 936890368 [M::mem_process_seqs] Processed 694632 reads in 117.935 CPU sec, 3.765 real sec sorting block 119, #reads = 1064782 mem = 536876688 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282871, 7, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 162, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (190.59, 113.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 273, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (295.70, 147.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 349, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1085) [M::mem_pestat] mean and std.dev: (334.14, 143.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1375) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692500 sequences (100000245 bp) in 2.326 CPU sec, 2.490 real sec... [M::process] read 693248 sequences (100000201 bp) in 1.480 CPU sec, 1.485 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 282836, 9, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 314, 904) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2442) [M::mem_pestat] mean and std.dev: (568.06, 597.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3211) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 274, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (297.15, 147.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 249, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014) [M::mem_pestat] mean and std.dev: (264.91, 111.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1292) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693402 reads in 179.809 CPU sec, 5.752 real sec [M::mem_process_seqs] Processed 693356 reads in 117.242 CPU sec, 3.739 real sec sorting block 120, #reads = 1064602 mem = 536877930 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282515, 10, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 254, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (254.73, 112.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 276, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833) [M::mem_pestat] mean and std.dev: (298.31, 147.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (270, 1168, 1578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4194) [M::mem_pestat] mean and std.dev: (1166.60, 900.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5502) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 281, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (276.83, 114.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692034 sequences (100000265 bp) in 2.250 CPU sec, 2.356 real sec... [M::process] read 691802 sequences (100000106 bp) in 1.490 CPU sec, 1.483 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282355, 11, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 191, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (197.29, 58.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 275, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (296.08, 145.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (561, 1472, 3735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10083) [M::mem_pestat] mean and std.dev: (1773.45, 1612.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13257) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 319, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1148) [M::mem_pestat] mean and std.dev: (342.25, 173.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1453) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692500 reads in 179.876 CPU sec, 5.736 real sec [M::mem_process_seqs] Processed 693248 reads in 119.644 CPU sec, 3.836 real sec sorting block 121, #reads = 1065852 mem = 536878876 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 282512, 3, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 264, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947) [M::mem_pestat] mean and std.dev: (286.06, 164.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1207) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 280, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (302.03, 149.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1069) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 240, 837) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2141) [M::mem_pestat] mean and std.dev: (478.38, 554.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2793) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 691172 sequences (100000151 bp) in 2.408 CPU sec, 2.527 real sec... [M::process] read 690306 sequences (100000191 bp) in 1.480 CPU sec, 1.484 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 282175, 25, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 250, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (267.07, 132.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 280, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848) [M::mem_pestat] mean and std.dev: (302.13, 148.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1070) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (477, 846, 1436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3354) [M::mem_pestat] mean and std.dev: (788.18, 463.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4313) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 265, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (263.40, 111.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692034 reads in 184.403 CPU sec, 6.080 real sec sorting block 122, #reads = 1062420 mem = 536878899 peak = 936890368 [M::mem_process_seqs] Processed 691802 reads in 118.611 CPU sec, 3.798 real sec sorting block 123, #reads = 1063231 mem = 536876123 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 282518, 2, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 307, 598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1440) [M::mem_pestat] mean and std.dev: (301.00, 211.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1861) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 284, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (304.95, 149.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1072) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 220, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (249.34, 110.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 690302 sequences (100000258 bp) in 2.329 CPU sec, 2.482 real sec... [M::process] read 689912 sequences (100000169 bp) in 1.470 CPU sec, 1.472 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282054, 1, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 245, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996) [M::mem_pestat] mean and std.dev: (240.07, 120.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1276) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 288, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (309.37, 152.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 320, 541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1229) [M::mem_pestat] mean and std.dev: (290.67, 141.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1573) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691172 reads in 179.962 CPU sec, 5.830 real sec [M::mem_process_seqs] Processed 690306 reads in 113.748 CPU sec, 3.639 real sec sorting block 124, #reads = 1065625 mem = 536881970 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282001, 12, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 179, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (174.37, 69.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 288, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (309.77, 152.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (5, 419, 634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1892) [M::mem_pestat] mean and std.dev: (317.27, 326.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2521) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 302, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (270.38, 102.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689564 sequences (100000018 bp) in 2.372 CPU sec, 2.536 real sec... [M::process] read 689074 sequences (100000093 bp) in 1.490 CPU sec, 1.486 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 281681, 6, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 237, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (233.69, 91.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 289, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (310.29, 152.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 247, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (266.64, 113.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690302 reads in 177.878 CPU sec, 5.677 real sec [M::mem_process_seqs] Processed 689912 reads in 117.662 CPU sec, 3.761 real sec sorting block 125, #reads = 1066801 mem = 536880965 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 281929, 15, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 209, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (216.25, 104.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 290, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (310.53, 151.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (802, 802, 802) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (802, 802) [M::mem_pestat] mean and std.dev: (802.00, 0.00) [M::mem_pestat] low and high boundaries for proper pairs: (802, 802) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 289, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026) [M::mem_pestat] mean and std.dev: (299.85, 140.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1295) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688702 sequences (100000179 bp) in 2.356 CPU sec, 2.508 real sec... [M::process] read 690796 sequences (100000004 bp) in 1.500 CPU sec, 1.500 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 281912, 9, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 335, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (276.38, 102.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1019) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 294, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (313.53, 153.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (262, 312, 2017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5527) [M::mem_pestat] mean and std.dev: (1019.56, 1205.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7282) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689564 reads in 172.363 CPU sec, 5.495 real sec sorting block 126, #reads = 1064572 mem = 536879973 peak = 936890368 [M::mem_process_seqs] Processed 689074 reads in 113.190 CPU sec, 3.643 real sec sorting block 127, #reads = 1066502 mem = 536878996 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281585, 18, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 295, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (308.67, 151.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1085) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 295, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (315.03, 153.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (565, 664, 1449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3217) [M::mem_pestat] mean and std.dev: (842.65, 643.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4101) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 264, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (266.40, 88.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689122 sequences (100000066 bp) in 2.312 CPU sec, 2.462 real sec... [M::process] read 687728 sequences (100000097 bp) in 1.470 CPU sec, 1.468 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 281799, 4, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 229, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (221.49, 102.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 284, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (307.96, 153.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1096) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 237, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (252.25, 115.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688702 reads in 180.444 CPU sec, 5.787 real sec [M::mem_process_seqs] Processed 690796 reads in 116.712 CPU sec, 3.732 real sec sorting block 128, #reads = 1064382 mem = 536880856 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 281845, 5, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 317, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013) [M::mem_pestat] mean and std.dev: (343.00, 221.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1282) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 293, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (312.95, 152.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 247, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (275.93, 143.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687356 sequences (100000053 bp) in 2.246 CPU sec, 2.371 real sec... [M::process] read 690102 sequences (100000263 bp) in 1.470 CPU sec, 1.467 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 280806, 14, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 213, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (277.62, 186.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 299, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (319.70, 156.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (718, 812, 1430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2854) [M::mem_pestat] mean and std.dev: (1044.79, 586.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3566) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 270, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (257.80, 88.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689122 reads in 168.774 CPU sec, 5.383 real sec [M::mem_process_seqs] Processed 687728 reads in 109.196 CPU sec, 3.726 real sec sorting block 129, #reads = 1066436 mem = 536884260 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 280693, 5, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 195, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (185.57, 65.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 302, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (322.22, 157.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1138) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (141, 240, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (230.31, 130.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686876 sequences (100000228 bp) in 2.383 CPU sec, 2.531 real sec... [M::process] read 687142 sequences (100000154 bp) in 1.450 CPU sec, 1.460 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 280929, 8, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 334, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075) [M::mem_pestat] mean and std.dev: (285.88, 129.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1362) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 284, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (309.49, 155.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1093) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (116, 184, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (215.71, 136.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687356 reads in 174.877 CPU sec, 5.820 real sec [M::mem_process_seqs] Processed 690102 reads in 110.529 CPU sec, 3.528 real sec sorting block 130, #reads = 1066763 mem = 536879603 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 280197, 13, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 253, 551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1355) [M::mem_pestat] mean and std.dev: (363.79, 287.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 306, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (326.81, 160.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (631, 1006, 1994) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4720) [M::mem_pestat] mean and std.dev: (1229.69, 973.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6083) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 293, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (290.66, 153.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 131, #reads = 1065672 mem = 536880319 peak = 936890368 [M::process] read 686814 sequences (100000061 bp) in 2.242 CPU sec, 2.340 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 280105, 8, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 249, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (228.07, 77.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 306, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (327.91, 163.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1168) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 250, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (305.62, 171.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686828 sequences (100000214 bp) in 1.520 CPU sec, 1.526 real sec... [M::mem_process_seqs] Processed 686876 reads in 174.250 CPU sec, 5.614 real sec [M::mem_process_seqs] Processed 687142 reads in 112.222 CPU sec, 3.572 real sec sorting block 132, #reads = 1064753 mem = 536880900 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 280216, 9, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 227, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1227) [M::mem_pestat] mean and std.dev: (237.12, 133.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 305, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (328.46, 163.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 300, 665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1637) [M::mem_pestat] mean and std.dev: (388.62, 324.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2123) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687040 sequences (100000078 bp) in 2.418 CPU sec, 2.575 real sec... [M::process] read 686224 sequences (100000198 bp) in 1.470 CPU sec, 1.475 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 280196, 8, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 316, 1441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3965) [M::mem_pestat] mean and std.dev: (996.33, 1278.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6112) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 306, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (329.67, 165.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1180) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 240, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (258.46, 119.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1100) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686814 reads in 169.128 CPU sec, 5.394 real sec [M::mem_process_seqs] Processed 686828 reads in 111.141 CPU sec, 3.544 real sec sorting block 133, #reads = 1065795 mem = 536879367 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 280275, 13, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (255, 338, 585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1245) [M::mem_pestat] mean and std.dev: (338.93, 153.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 306, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (329.18, 164.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1179) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (849, 975, 3037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7413) [M::mem_pestat] mean and std.dev: (1542.00, 1067.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9601) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 367, 575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1343) [M::mem_pestat] mean and std.dev: (393.97, 289.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1727) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685820 sequences (100000288 bp) in 2.443 CPU sec, 2.603 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 280072, 11, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 304, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (296.94, 118.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 308, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (330.26, 163.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1170) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (254, 254, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (82, 512) [M::mem_pestat] mean and std.dev: (289.62, 82.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 282, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846) [M::mem_pestat] mean and std.dev: (313.18, 153.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685906 sequences (100000107 bp) in 1.470 CPU sec, 1.468 real sec... [M::mem_process_seqs] Processed 687040 reads in 165.329 CPU sec, 5.291 real sec [M::mem_process_seqs] Processed 686224 reads in 108.324 CPU sec, 3.436 real sec sorting block 134, #reads = 1065443 mem = 536883527 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 279175, 11, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 246, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (236.90, 115.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 310, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931) [M::mem_pestat] mean and std.dev: (332.13, 164.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1175) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (332, 509, 1401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3539) [M::mem_pestat] mean and std.dev: (722.40, 625.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4608) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 335, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (315.23, 149.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685526 sequences (100000137 bp) in 2.313 CPU sec, 2.453 real sec... [M::process] read 687042 sequences (100000165 bp) in 1.500 CPU sec, 1.506 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 280108, 18, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 279, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (247.17, 121.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 312, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (334.27, 166.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1188) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (382, 927, 1856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4804) [M::mem_pestat] mean and std.dev: (938.00, 766.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6278) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (244, 350, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976) [M::mem_pestat] mean and std.dev: (371.49, 186.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1220) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685820 reads in 172.085 CPU sec, 5.724 real sec sorting block 135, #reads = 1065371 mem = 536879966 peak = 936890368 [M::mem_process_seqs] Processed 685906 reads in 112.990 CPU sec, 3.612 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 279723, 7, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 288, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (253.31, 76.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 313, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936) [M::mem_pestat] mean and std.dev: (334.44, 165.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1181) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 286, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021) [M::mem_pestat] mean and std.dev: (313.73, 166.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1296) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 136, #reads = 1063302 mem = 536879614 peak = 936890368 [M::process] read 693106 sequences (100000033 bp) in 2.295 CPU sec, 2.428 real sec... [M::process] read 692878 sequences (100000046 bp) in 1.459 CPU sec, 1.454 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 280141, 11, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 316, 625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1509) [M::mem_pestat] mean and std.dev: (389.57, 331.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1951) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 306, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (328.35, 163.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1175) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (817, 1819, 2020) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4426) [M::mem_pestat] mean and std.dev: (1621.82, 948.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5629) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 251, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (265.27, 117.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1016) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685526 reads in 183.223 CPU sec, 5.872 real sec [M::mem_process_seqs] Processed 687042 reads in 128.190 CPU sec, 4.101 real sec sorting block 137, #reads = 1062722 mem = 536878739 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 281852, 13, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 332, 827) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2011) [M::mem_pestat] mean and std.dev: (477.21, 471.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 274, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841) [M::mem_pestat] mean and std.dev: (299.70, 152.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1062) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1117, 2295, 4261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10549) [M::mem_pestat] mean and std.dev: (2748.54, 2227.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13693) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 261, 564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1280) [M::mem_pestat] mean and std.dev: (337.97, 178.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1638) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691718 sequences (100000084 bp) in 2.374 CPU sec, 2.555 real sec... [M::process] read 690692 sequences (100000123 bp) in 1.460 CPU sec, 1.456 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 282306, 13, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 269, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1058) [M::mem_pestat] mean and std.dev: (284.06, 130.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 275, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (301.44, 153.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1070) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (625, 697, 1998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4744) [M::mem_pestat] mean and std.dev: (1117.67, 1176.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6117) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (132, 182, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (194.03, 92.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693106 reads in 180.152 CPU sec, 5.748 real sec [M::mem_process_seqs] Processed 692878 reads in 117.351 CPU sec, 3.757 real sec sorting block 138, #reads = 1057181 mem = 536881958 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 282864, 14, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 210, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (239.11, 147.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1006) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 281, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (305.47, 153.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1082) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (331, 774, 1301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3241) [M::mem_pestat] mean and std.dev: (648.08, 420.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4211) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 272, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (292.44, 164.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1181) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 690762 sequences (100000073 bp) in 2.345 CPU sec, 2.457 real sec... [M::process] read 690152 sequences (100000212 bp) in 1.460 CPU sec, 1.467 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282357, 9, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 196, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (185.06, 61.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 285, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (307.21, 152.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1080) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 294, 724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1730) [M::mem_pestat] mean and std.dev: (396.00, 304.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2233) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691718 reads in 167.818 CPU sec, 5.353 real sec sorting block 139, #reads = 1070143 mem = 536879801 peak = 936890368 [M::mem_process_seqs] Processed 690692 reads in 107.622 CPU sec, 3.442 real sec sorting block 140, #reads = 1069413 mem = 536879192 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 281964, 10, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 180, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (209.19, 101.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 286, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (308.34, 153.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1088) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (162, 1006, 6185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18231) [M::mem_pestat] mean and std.dev: (2207.20, 2625.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 24254) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 333, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (348.65, 168.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689152 sequences (100000180 bp) in 2.325 CPU sec, 2.435 real sec... [M::process] read 689116 sequences (100000052 bp) in 1.460 CPU sec, 1.458 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282153, 7, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 302, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986) [M::mem_pestat] mean and std.dev: (284.90, 150.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1248) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 289, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (311.49, 155.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 321, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1065) [M::mem_pestat] mean and std.dev: (272.62, 139.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1367) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690762 reads in 168.662 CPU sec, 5.632 real sec [M::mem_process_seqs] Processed 690152 reads in 109.120 CPU sec, 3.505 real sec sorting block 141, #reads = 1070407 mem = 536880654 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 281015, 6, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 274, 542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1324) [M::mem_pestat] mean and std.dev: (346.08, 240.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1715) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 294, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (315.02, 155.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 309, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (334.03, 162.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689316 sequences (100000107 bp) in 2.327 CPU sec, 2.496 real sec... [M::process] read 688928 sequences (100000017 bp) in 1.450 CPU sec, 1.445 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 281494, 5, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 378, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948) [M::mem_pestat] mean and std.dev: (331.77, 145.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1199) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 294, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (315.73, 156.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 258, 1275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3465) [M::mem_pestat] mean and std.dev: (412.74, 394.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4560) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689152 reads in 163.667 CPU sec, 5.225 real sec [M::mem_process_seqs] Processed 689116 reads in 106.652 CPU sec, 3.407 real sec sorting block 142, #reads = 1069293 mem = 536879256 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 282226, 8, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 236, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1087) [M::mem_pestat] mean and std.dev: (310.85, 204.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 296, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (318.31, 159.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 192, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (222.26, 89.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1021) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688370 sequences (100000053 bp) in 2.336 CPU sec, 2.447 real sec... [M::process] read 688400 sequences (100000019 bp) in 1.460 CPU sec, 1.460 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 281746, 6, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 180, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (239.59, 151.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1000) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 295, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (318.10, 158.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 303, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (295.00, 131.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1089) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689316 reads in 161.212 CPU sec, 5.147 real sec [M::mem_process_seqs] Processed 688928 reads in 105.171 CPU sec, 3.355 real sec sorting block 143, #reads = 1071029 mem = 536880027 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 281423, 8, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 293, 699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1683) [M::mem_pestat] mean and std.dev: (452.86, 367.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2175) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 298, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (320.14, 159.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 343, 640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1482) [M::mem_pestat] mean and std.dev: (405.55, 286.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1903) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 144, #reads = 1070731 mem = 536878958 peak = 936890368 [M::process] read 688220 sequences (100000075 bp) in 2.395 CPU sec, 2.539 real sec... [M::process] read 688236 sequences (100000013 bp) in 1.480 CPU sec, 1.483 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 280987, 8, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 188, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (210.65, 93.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 299, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (320.28, 159.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 335, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015) [M::mem_pestat] mean and std.dev: (330.13, 173.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1289) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688370 reads in 162.592 CPU sec, 5.187 real sec [M::mem_process_seqs] Processed 688400 reads in 106.513 CPU sec, 3.400 real sec sorting block 145, #reads = 1070512 mem = 536883491 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281289, 10, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 187, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004) [M::mem_pestat] mean and std.dev: (277.69, 155.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1285) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 299, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (319.55, 157.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (501, 854, 1195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2583) [M::mem_pestat] mean and std.dev: (843.78, 412.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3277) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 259, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840) [M::mem_pestat] mean and std.dev: (284.92, 145.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688594 sequences (100000141 bp) in 2.408 CPU sec, 2.536 real sec... [M::process] read 688142 sequences (100000215 bp) in 1.470 CPU sec, 1.467 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 280862, 6, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 331, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982) [M::mem_pestat] mean and std.dev: (279.42, 158.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1265) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 298, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (319.63, 158.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (261, 290, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035) [M::mem_pestat] mean and std.dev: (373.18, 238.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1327) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688220 reads in 165.519 CPU sec, 5.289 real sec [M::mem_process_seqs] Processed 688236 reads in 110.400 CPU sec, 3.525 real sec sorting block 146, #reads = 1069150 mem = 536879794 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 281361, 12, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 367, 654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1582) [M::mem_pestat] mean and std.dev: (427.45, 272.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2046) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 296, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (316.58, 155.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1088, 1685, 1723) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2993) [M::mem_pestat] mean and std.dev: (1163.09, 619.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3641) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 354, 560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1318) [M::mem_pestat] mean and std.dev: (328.56, 157.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1697) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687500 sequences (100000015 bp) in 2.351 CPU sec, 2.483 real sec... [M::process] read 686948 sequences (100000123 bp) in 1.450 CPU sec, 1.455 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 281337, 7, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 252, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018) [M::mem_pestat] mean and std.dev: (299.82, 188.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1287) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 298, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (320.07, 158.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 243, 643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1553) [M::mem_pestat] mean and std.dev: (375.86, 331.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2008) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688594 reads in 169.283 CPU sec, 5.413 real sec [M::mem_process_seqs] Processed 688142 reads in 108.496 CPU sec, 3.475 real sec sorting block 147, #reads = 1067935 mem = 536880740 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 280603, 16, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 455, 819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2023) [M::mem_pestat] mean and std.dev: (530.40, 348.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 302, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (322.84, 159.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1138) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (118, 515, 858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2338) [M::mem_pestat] mean and std.dev: (564.53, 647.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3155) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (132, 210, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (218.34, 125.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686774 sequences (100000010 bp) in 2.856 CPU sec, 3.097 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 280152, 9, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 304, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009) [M::mem_pestat] mean and std.dev: (288.65, 167.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1284) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 303, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (322.45, 156.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (277, 329, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (333.47, 118.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687452 sequences (100000252 bp) in 1.514 CPU sec, 1.520 real sec... [M::mem_process_seqs] Processed 687500 reads in 171.547 CPU sec, 5.466 real sec sorting block 148, #reads = 1067968 mem = 536883547 peak = 936890368 [M::mem_process_seqs] Processed 686948 reads in 110.840 CPU sec, 3.539 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 280163, 8, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 219, 512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1278) [M::mem_pestat] mean and std.dev: (284.78, 227.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 306, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (325.86, 159.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 218, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (15, 455) [M::mem_pestat] mean and std.dev: (217.96, 57.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 149, #reads = 1066459 mem = 536878997 peak = 936890368 [M::process] read 686950 sequences (100000183 bp) in 2.465 CPU sec, 2.592 real sec... [M::process] read 686436 sequences (100000219 bp) in 1.460 CPU sec, 1.464 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 280807, 10, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 313, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025) [M::mem_pestat] mean and std.dev: (283.70, 100.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1293) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 304, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (324.68, 160.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (337, 750, 750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1576) [M::mem_pestat] mean and std.dev: (614.33, 338.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1989) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 248, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (282.65, 166.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686774 reads in 166.621 CPU sec, 5.320 real sec [M::mem_process_seqs] Processed 687452 reads in 109.614 CPU sec, 3.503 real sec sorting block 150, #reads = 1067622 mem = 536881098 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 280669, 6, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 160, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (188.57, 75.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 305, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (326.67, 162.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (143, 259, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (262.00, 159.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686398 sequences (100000097 bp) in 2.287 CPU sec, 2.363 real sec... [M::process] read 686620 sequences (100000075 bp) in 1.470 CPU sec, 1.472 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 280184, 4, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 297, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010) [M::mem_pestat] mean and std.dev: (310.78, 177.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1289) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 307, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (328.40, 162.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1158) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 297, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (310.15, 170.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1069) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686950 reads in 165.389 CPU sec, 5.283 real sec [M::mem_process_seqs] Processed 686436 reads in 109.234 CPU sec, 3.486 real sec sorting block 151, #reads = 1067785 mem = 536879789 peak = 936890368 [M::process] read 686208 sequences (100000151 bp) in 2.308 CPU sec, 2.394 real sec... [M::process] read 686292 sequences (100000202 bp) in 1.450 CPU sec, 1.455 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 279598, 7, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 284, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029) [M::mem_pestat] mean and std.dev: (285.64, 170.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1318) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 304, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (325.58, 160.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 390, 607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1385) [M::mem_pestat] mean and std.dev: (400.66, 251.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1774) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686620 reads in 92.212 CPU sec, 2.966 real sec sorting block 152, #reads = 1067576 mem = 536878869 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 279821, 11, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 343, 574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1314) [M::mem_pestat] mean and std.dev: (365.81, 228.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 307, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (328.17, 161.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (142, 645, 3054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8878) [M::mem_pestat] mean and std.dev: (1355.55, 1456.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11790) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 328, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846) [M::mem_pestat] mean and std.dev: (322.98, 128.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 280091, 11, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 262, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1313) [M::mem_pestat] mean and std.dev: (251.81, 140.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 308, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (330.28, 163.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1032, 1169, 1174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (748, 1458) [M::mem_pestat] mean and std.dev: (1053.00, 168.80) [M::mem_pestat] low and high boundaries for proper pairs: (378, 1728) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 333, 569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1261) [M::mem_pestat] mean and std.dev: (375.16, 236.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1607) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686506 sequences (100000264 bp) in 1.709 CPU sec, 1.716 real sec... [M::mem_process_seqs] Processed 686398 reads in 264.065 CPU sec, 8.462 real sec [M::mem_process_seqs] Processed 686208 reads in 133.611 CPU sec, 4.356 real sec sorting block 153, #reads = 1066398 mem = 536878483 peak = 936890368 [M::process] read 686470 sequences (100000024 bp) in 2.288 CPU sec, 2.395 real sec... [M::process] read 686330 sequences (100000170 bp) in 1.440 CPU sec, 1.447 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 279819, 4, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 309, 808) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2038) [M::mem_pestat] mean and std.dev: (437.20, 274.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 305, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (326.53, 161.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (126, 227, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837) [M::mem_pestat] mean and std.dev: (240.31, 131.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1074) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686506 reads in 95.051 CPU sec, 3.061 real sec sorting block 154, #reads = 1065383 mem = 536879022 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 279760, 6, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 308, 550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1278) [M::mem_pestat] mean and std.dev: (338.94, 216.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 306, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (327.23, 161.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (250, 319, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (292.33, 93.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 280009, 12, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 188, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (196.39, 59.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 304, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (326.88, 161.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (356, 727, 2481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6731) [M::mem_pestat] mean and std.dev: (1328.33, 1170.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8856) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (274, 377, 821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1915) [M::mem_pestat] mean and std.dev: (462.83, 323.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2462) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686520 sequences (100000067 bp) in 1.721 CPU sec, 1.731 real sec... [M::mem_process_seqs] Processed 686292 reads in 272.101 CPU sec, 8.747 real sec [M::mem_process_seqs] Processed 686470 reads in 135.118 CPU sec, 4.398 real sec sorting block 155, #reads = 1065680 mem = 536879176 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 279798, 10, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 264, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (242.21, 83.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 306, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (327.65, 161.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (194, 324, 682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1658) [M::mem_pestat] mean and std.dev: (291.00, 243.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2146) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 238, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (286.25, 133.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686976 sequences (100000236 bp) in 2.413 CPU sec, 2.636 real sec... [M::process] read 686400 sequences (100000221 bp) in 1.440 CPU sec, 1.446 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 280245, 10, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 249, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (234.81, 90.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 304, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (326.98, 162.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (268, 2599, 2599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7261) [M::mem_pestat] mean and std.dev: (2282.10, 2498.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12278) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (260, 339, 670) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1490) [M::mem_pestat] mean and std.dev: (412.40, 268.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1900) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686330 reads in 171.999 CPU sec, 5.490 real sec sorting block 156, #reads = 1065407 mem = 536876828 peak = 936890368 [M::mem_process_seqs] Processed 686520 reads in 113.787 CPU sec, 4.002 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 280591, 13, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 240, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (270.57, 164.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 302, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (325.65, 163.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (251, 251, 803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1907) [M::mem_pestat] mean and std.dev: (320.17, 250.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2459) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 341, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (314.44, 147.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1167) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686394 sequences (100000033 bp) in 3.089 CPU sec, 3.460 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 279829, 14, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 197, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (197.58, 66.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 306, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (328.67, 163.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (594, 1234, 2471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6225) [M::mem_pestat] mean and std.dev: (1546.86, 1213.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8102) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 312, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962) [M::mem_pestat] mean and std.dev: (326.05, 191.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1217) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685844 sequences (100000030 bp) in 1.539 CPU sec, 1.548 real sec... [M::mem_process_seqs] Processed 686976 reads in 182.737 CPU sec, 6.186 real sec sorting block 157, #reads = 1065117 mem = 536881069 peak = 936890368 [M::mem_process_seqs] Processed 686400 reads in 118.731 CPU sec, 3.792 real sec sorting block 158, #reads = 1065511 mem = 536878522 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 279716, 8, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 377, 546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1166) [M::mem_pestat] mean and std.dev: (364.26, 198.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1476) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 304, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (325.86, 161.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 273, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (246.11, 99.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 690582 sequences (100000059 bp) in 2.540 CPU sec, 2.745 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 279313, 6, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 275, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (285.07, 132.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1163) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 308, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (330.94, 165.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1163) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 259, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794) [M::mem_pestat] mean and std.dev: (264.44, 129.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 698474 sequences (100000085 bp) in 1.520 CPU sec, 1.524 real sec... [M::mem_process_seqs] Processed 686394 reads in 184.447 CPU sec, 5.916 real sec [M::mem_process_seqs] Processed 685844 reads in 125.268 CPU sec, 4.001 real sec sorting block 159, #reads = 1062654 mem = 536880698 peak = 936890368 [M::process] read 696024 sequences (100000202 bp) in 2.343 CPU sec, 2.514 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 281112, 11, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 234, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1294) [M::mem_pestat] mean and std.dev: (359.27, 334.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 285, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (311.73, 159.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (431, 698, 810) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1568) [M::mem_pestat] mean and std.dev: (547.80, 295.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1947) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 361, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046) [M::mem_pestat] mean and std.dev: (346.32, 170.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1328) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695198 sequences (100000210 bp) in 1.490 CPU sec, 1.494 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 284327, 3, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 224, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (231.32, 73.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 252, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (279.23, 142.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 301, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (308.76, 158.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690582 reads in 201.788 CPU sec, 6.603 real sec sorting block 160, #reads = 1054265 mem = 536878975 peak = 936890368 [M::process] read 693780 sequences (100000009 bp) in 1.760 CPU sec, 1.761 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 283073, 8, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 320, 626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1404) [M::mem_pestat] mean and std.dev: (391.60, 203.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 266, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (291.22, 146.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 312, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (272.53, 144.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695198 reads in 100.195 CPU sec, 3.405 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 283477, 5, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 214, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (209.16, 76.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 261, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (287.17, 144.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1019) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 298, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001) [M::mem_pestat] mean and std.dev: (345.56, 253.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1360) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696024 reads in 207.223 CPU sec, 7.417 real sec sorting block 161, #reads = 1064606 mem = 536881765 peak = 936890368 [M::process] read 692304 sequences (100000182 bp) in 2.209 CPU sec, 2.346 real sec... [M::mem_process_seqs] Processed 698474 reads in 388.975 CPU sec, 13.231 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282838, 10, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 331, 662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1604) [M::mem_pestat] mean and std.dev: (407.13, 293.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2075) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 272, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (295.03, 146.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (182, 1054, 2312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6572) [M::mem_pestat] mean and std.dev: (1398.00, 1245.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8702) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 258, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (241.09, 86.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 162, #reads = 1063537 mem = 536887552 peak = 936890368 [M::process] read 691976 sequences (100000051 bp) in 2.298 CPU sec, 2.510 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 282251, 17, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 216, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (238.27, 146.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 278, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (298.27, 146.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (300, 450, 2411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6633) [M::mem_pestat] mean and std.dev: (1068.12, 912.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8744) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 240, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000) [M::mem_pestat] mean and std.dev: (273.80, 129.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1277) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691594 sequences (100000202 bp) in 1.490 CPU sec, 1.491 real sec... [M::mem_process_seqs] Processed 693780 reads in 218.164 CPU sec, 6.984 real sec [M::mem_process_seqs] Processed 692304 reads in 125.947 CPU sec, 4.085 real sec sorting block 163, #reads = 1064273 mem = 536881999 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282055, 11, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 165, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1135) [M::mem_pestat] mean and std.dev: (255.88, 215.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 279, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (300.52, 147.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1057) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (697, 2124, 2888) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7270) [M::mem_pestat] mean and std.dev: (2263.45, 1974.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10162) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 264, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (289.03, 130.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1079) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691830 sequences (100000262 bp) in 2.522 CPU sec, 2.753 real sec... [M::process] read 690880 sequences (100000146 bp) in 1.500 CPU sec, 1.499 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 282172, 7, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 256, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966) [M::mem_pestat] mean and std.dev: (266.07, 127.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1234) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 281, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (303.18, 150.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1074) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 319, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (294.58, 93.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1075) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691976 reads in 198.956 CPU sec, 6.440 real sec [M::mem_process_seqs] Processed 691594 reads in 129.890 CPU sec, 4.159 real sec sorting block 164, #reads = 1063996 mem = 536877978 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 281442, 10, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 238, 829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2215) [M::mem_pestat] mean and std.dev: (481.54, 518.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2908) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 281, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (305.01, 152.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1089) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (267, 1762, 1762) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4752) [M::mem_pestat] mean and std.dev: (968.00, 728.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6247) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 295, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (256.89, 99.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689978 sequences (100000147 bp) in 2.228 CPU sec, 2.409 real sec... [M::process] read 689642 sequences (100000153 bp) in 1.490 CPU sec, 1.488 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282079, 16, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 305, 610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1472) [M::mem_pestat] mean and std.dev: (371.96, 266.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1903) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 284, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (307.91, 153.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1096) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (134, 714, 1402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3938) [M::mem_pestat] mean and std.dev: (650.56, 550.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5206) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 241, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (236.97, 77.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691830 reads in 185.500 CPU sec, 5.951 real sec sorting block 165, #reads = 1061839 mem = 536878002 peak = 936890368 [M::mem_process_seqs] Processed 690880 reads in 118.692 CPU sec, 3.946 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 281172, 6, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 284, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (248.53, 97.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 288, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (310.54, 154.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 328, 945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2367) [M::mem_pestat] mean and std.dev: (428.65, 311.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3078) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 166, #reads = 1064140 mem = 536878822 peak = 936890368 [M::process] read 689472 sequences (100000268 bp) in 2.654 CPU sec, 2.901 real sec... [M::process] read 689972 sequences (100000073 bp) in 1.510 CPU sec, 1.512 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 281189, 13, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 319, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956) [M::mem_pestat] mean and std.dev: (277.24, 139.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1226) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 290, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (313.23, 155.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1253, 1377, 1415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (929, 1739) [M::mem_pestat] mean and std.dev: (1337.30, 119.74) [M::mem_pestat] low and high boundaries for proper pairs: (767, 1901) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 236, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (243.04, 96.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689978 reads in 183.914 CPU sec, 6.026 real sec [M::mem_process_seqs] Processed 689642 reads in 118.289 CPU sec, 3.841 real sec sorting block 167, #reads = 1065380 mem = 536880445 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 280779, 8, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 317, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (387.27, 235.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1328) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 290, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (312.80, 154.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 338, 573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1297) [M::mem_pestat] mean and std.dev: (335.72, 207.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1659) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689370 sequences (100000239 bp) in 2.243 CPU sec, 2.437 real sec... [M::process] read 687462 sequences (100000111 bp) in 1.550 CPU sec, 1.546 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 281011, 5, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 608, 1158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3104) [M::mem_pestat] mean and std.dev: (526.34, 421.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4077) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 286, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (307.55, 150.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1085) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (257, 343, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (311.41, 119.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689472 reads in 183.870 CPU sec, 5.960 real sec sorting block 168, #reads = 1065118 mem = 536878844 peak = 936890368 [M::process] read 686686 sequences (100000187 bp) in 1.688 CPU sec, 1.695 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 280943, 11, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 293, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1175) [M::mem_pestat] mean and std.dev: (277.25, 166.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 290, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (311.62, 153.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1100) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (322, 457, 1310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3286) [M::mem_pestat] mean and std.dev: (760.45, 632.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4274) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 272, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (303.21, 140.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1143) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689370 reads in 104.692 CPU sec, 3.344 real sec [M::mem_process_seqs] Processed 689972 reads in 254.324 CPU sec, 8.142 real sec sorting block 169, #reads = 1064381 mem = 536878533 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 280061, 16, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 283, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1098) [M::mem_pestat] mean and std.dev: (350.52, 249.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 299, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (318.80, 154.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (743, 1554, 2319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5471) [M::mem_pestat] mean and std.dev: (1337.19, 817.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7047) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (246, 317, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (306.80, 125.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687968 sequences (100000028 bp) in 2.224 CPU sec, 2.432 real sec... [M::process] read 688254 sequences (100000107 bp) in 1.460 CPU sec, 1.465 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 279981, 14, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 221, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (247.74, 176.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 306, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (323.92, 156.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (126, 1110, 2491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7221) [M::mem_pestat] mean and std.dev: (1261.21, 1273.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9586) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 298, 679) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1573) [M::mem_pestat] mean and std.dev: (394.71, 280.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2020) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687462 reads in 197.443 CPU sec, 6.316 real sec [M::mem_process_seqs] Processed 686686 reads in 125.346 CPU sec, 4.024 real sec sorting block 170, #reads = 1061259 mem = 536879414 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 280053, 20, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 244, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (239.15, 99.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1181) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 296, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (316.52, 154.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (117, 584, 1803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5175) [M::mem_pestat] mean and std.dev: (970.20, 928.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6861) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 260, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040) [M::mem_pestat] mean and std.dev: (274.44, 146.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1333) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687832 sequences (100000039 bp) in 2.388 CPU sec, 2.631 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 280503, 11, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 279, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (266.56, 122.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 293, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (314.60, 153.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (270, 598, 598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1254) [M::mem_pestat] mean and std.dev: (396.89, 227.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1582) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 312, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1114) [M::mem_pestat] mean and std.dev: (349.73, 236.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1425) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687352 sequences (100000024 bp) in 1.450 CPU sec, 1.459 real sec... [M::mem_process_seqs] Processed 687968 reads in 178.926 CPU sec, 5.734 real sec sorting block 171, #reads = 1063083 mem = 536878746 peak = 936890368 [M::mem_process_seqs] Processed 688254 reads in 125.027 CPU sec, 4.003 real sec sorting block 172, #reads = 1063128 mem = 536882018 peak = 936890368 [M::process] read 686954 sequences (100000279 bp) in 2.204 CPU sec, 2.349 real sec... [M::process] read 687176 sequences (100000021 bp) in 1.470 CPU sec, 1.474 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 280289, 2, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 249, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1230) [M::mem_pestat] mean and std.dev: (290.48, 182.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 298, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (318.47, 155.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 286, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921) [M::mem_pestat] mean and std.dev: (285.27, 157.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1173) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687352 reads in 93.749 CPU sec, 3.008 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 280397, 7, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 276, 779) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2025) [M::mem_pestat] mean and std.dev: (433.12, 421.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 296, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (317.14, 155.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 287, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (245.93, 96.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1073) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687132 sequences (100000131 bp) in 1.646 CPU sec, 1.650 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 280125, 10, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 391, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1077) [M::mem_pestat] mean and std.dev: (318.05, 164.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1370) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 301, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (321.10, 156.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1123) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (295, 1872, 1904) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5122) [M::mem_pestat] mean and std.dev: (1231.20, 860.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6731) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (256, 338, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (320.94, 113.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687832 reads in 267.756 CPU sec, 8.590 real sec [M::mem_process_seqs] Processed 686954 reads in 132.124 CPU sec, 4.318 real sec sorting block 173, #reads = 1064972 mem = 536883769 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 280165, 19, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 300, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936) [M::mem_pestat] mean and std.dev: (279.43, 128.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1177) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 300, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (320.14, 156.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (380, 668, 999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2237) [M::mem_pestat] mean and std.dev: (698.84, 500.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2856) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 200, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (176.78, 46.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687102 sequences (100000007 bp) in 2.439 CPU sec, 2.700 real sec... [M::process] read 686766 sequences (100000059 bp) in 1.440 CPU sec, 1.433 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 280211, 6, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 199, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (214.11, 92.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 299, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (320.66, 156.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 314, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963) [M::mem_pestat] mean and std.dev: (292.42, 144.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1204) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687176 reads in 172.614 CPU sec, 5.547 real sec sorting block 174, #reads = 1065019 mem = 536882245 peak = 936890368 [M::mem_process_seqs] Processed 687132 reads in 110.838 CPU sec, 3.540 real sec sorting block 175, #reads = 1066741 mem = 536878175 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 280433, 6, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 307, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (267.70, 97.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 302, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (322.87, 158.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 351, 662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1508) [M::mem_pestat] mean and std.dev: (373.11, 227.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1931) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686734 sequences (100000268 bp) in 2.225 CPU sec, 2.457 real sec... [M::process] read 686968 sequences (100000057 bp) in 1.460 CPU sec, 1.465 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 279983, 7, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 224, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (209.43, 81.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 300, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (323.54, 159.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 386, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1136) [M::mem_pestat] mean and std.dev: (350.86, 210.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1443) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687102 reads in 164.403 CPU sec, 5.245 real sec [M::mem_process_seqs] Processed 686766 reads in 114.273 CPU sec, 3.667 real sec sorting block 176, #reads = 1066023 mem = 536879990 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 280144, 19, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 296, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1282) [M::mem_pestat] mean and std.dev: (360.86, 262.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 302, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (324.75, 159.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (645, 1030, 2053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4869) [M::mem_pestat] mean and std.dev: (1153.83, 758.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6277) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 236, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (234.52, 72.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686234 sequences (100000222 bp) in 2.207 CPU sec, 2.384 real sec... [M::process] read 684738 sequences (100000175 bp) in 1.450 CPU sec, 1.452 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 280151, 5, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 286, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833) [M::mem_pestat] mean and std.dev: (274.07, 151.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 301, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (323.99, 160.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 353, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1174) [M::mem_pestat] mean and std.dev: (292.47, 170.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1499) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686734 reads in 164.079 CPU sec, 5.236 real sec [M::mem_process_seqs] Processed 686968 reads in 106.781 CPU sec, 3.443 real sec sorting block 177, #reads = 1066811 mem = 536879289 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 280383, 15, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 293, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (246.19, 96.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 305, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (327.14, 161.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (232, 1675, 2416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6784) [M::mem_pestat] mean and std.dev: (1353.27, 1032.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8968) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 352, 625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1401) [M::mem_pestat] mean and std.dev: (410.34, 238.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1789) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685930 sequences (100000199 bp) in 2.118 CPU sec, 2.271 real sec... [M::process] read 685962 sequences (100000256 bp) in 1.480 CPU sec, 1.479 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 279378, 12, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (281.12, 175.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 984) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 316, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (336.27, 164.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1183) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (837, 2439, 4625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12201) [M::mem_pestat] mean and std.dev: (2430.25, 1980.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15989) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 278, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060) [M::mem_pestat] mean and std.dev: (309.14, 194.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1345) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686234 reads in 176.480 CPU sec, 5.697 real sec sorting block 178, #reads = 1066653 mem = 536885225 peak = 936890368 [M::mem_process_seqs] Processed 684738 reads in 108.801 CPU sec, 3.483 real sec [M::process] read 274414 sequences (39920936 bp) in 1.206 CPU sec, 1.288 real sec... sorting block 179, #reads = 1064452 mem = 536879074 peak = 936890368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 279686, 12, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 384, 478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052) [M::mem_pestat] mean and std.dev: (431.79, 290.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 308, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (330.09, 163.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1159) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (77, 633, 720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2006) [M::mem_pestat] mean and std.dev: (443.55, 362.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2649) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 395, 563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1205) [M::mem_pestat] mean and std.dev: (349.68, 180.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1526) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 279807, 15, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 261, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (275.94, 148.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 307, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (328.74, 161.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1155) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1447, 1698, 2858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5680) [M::mem_pestat] mean and std.dev: (2086.73, 1565.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8348) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 305, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (320.63, 165.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 112223, 6, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 400, 625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1525) [M::mem_pestat] mean and std.dev: (354.67, 174.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1975) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 300, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (322.28, 159.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 257, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (243.14, 99.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685930 reads in 197.554 CPU sec, 6.411 real sec [M::mem_process_seqs] Processed 685962 reads in 137.890 CPU sec, 4.462 real sec [M::mem_process_seqs] Processed 274414 reads in 145.765 CPU sec, 4.730 real sec sorting block 180, #reads = 1065292 mem = 536879776 peak = 936890368 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 192739450 overall: 69864366080 mem 28590.104 user 936.614 sys 988.590 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1029_T05-T1-TDNA-1_A23MHWWLT4_1.L007\tPU:L007\tSM:1029_T05-T1-TDNA-1_A23MHWWLT4_1\tLB:1029_T05-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1029_T05-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 1029_T05-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 988.617 sec; CPU: 29526.721 sec sorting block 181, #reads = 761289 mem = 385140104 peak = 936890368 uniq header count 1 merging 182 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 384 algo: util-sort license: sentieon:util=1 output file size: 10986311070 output reads: 194092243 bam_mem_sort: 182 calls 94.893 user 1.429 sys 96.618 real bam_write: 182 calls 128.709 user 4.507 sys 135.000 real execute: 1 calls 43.629 user 2.862 sys 1051.921 real merge_files: 1 calls 43.602 user 2.821 sys 62.145 real parse_chunk: 2908 calls 104.325 user 30.962 sys 137.924 real read_chunk: 7856 calls 85.534 user 67.189 sys 891.210 real sort_block: 1 calls 66.381 user 66.315 sys 863.036 real write_chunk: 4946 calls 43.234 user 2.371 sys 61.017 real overall: 3293958144 mem 2478.666 user 129.082 sys 1051.942 real ls: cannot access '*.cram': No such file or directory