This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_cfy-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1508.994 CPU sec, 53.543 real sec
[M::process] read 690388 sequences (100000247 bp) in 1.605 CPU sec, 1.609 real sec...
[M::process] read 688998 sequences (100000145 bp) in 1.768 CPU sec, 1.782 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287443, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 255, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (276.96, 130.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688430 sequences (100000188 bp) in 1.803 CPU sec, 1.821 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287579, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 260, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (282.19, 133.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 690388 reads in 168.262 CPU sec, 5.369 real sec
[M::process] read 687948 sequences (100000023 bp) in 1.831 CPU sec, 1.843 real sec...
[M::mem_process_seqs] Processed 688998 reads in 113.862 CPU sec, 3.623 real sec
sorting block 0, #reads = 1089391 mem = 536877948 peak = 600649728
[M::process] read 687586 sequences (100000162 bp) in 2.047 CPU sec, 2.102 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287004, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 264, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (285.92, 135.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687324 sequences (100000144 bp) in 2.010 CPU sec, 2.013 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286718, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 271, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (291.12, 136.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286642, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 268, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (288.44, 135.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 962)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688430 reads in 236.872 CPU sec, 7.720 real sec
[M::mem_process_seqs] Processed 687586 reads in 182.989 CPU sec, 6.023 real sec
[M::mem_process_seqs] Processed 687948 reads in 202.834 CPU sec, 6.694 real sec
sorting block 1, #reads = 1089237 mem = 536879225 peak = 731197440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286640, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 272, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (292.77, 138.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687456 sequences (100000214 bp) in 2.659 CPU sec, 2.766 real sec...
[M::mem_process_seqs] Processed 687324 reads in 99.685 CPU sec, 3.304 real sec
sorting block 2, #reads = 1088623 mem = 536884444 peak = 838705152
[M::process] read 687196 sequences (100000157 bp) in 2.506 CPU sec, 2.586 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287094, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 272, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (292.26, 137.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686612 sequences (100000070 bp) in 2.193 CPU sec, 2.230 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286831, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 273, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (293.89, 138.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687456 reads in 172.513 CPU sec, 5.502 real sec
[M::mem_process_seqs] Processed 687196 reads in 115.985 CPU sec, 3.684 real sec
sorting block 3, #reads = 1087270 mem = 536878338 peak = 897503232
[M::process] read 686388 sequences (100000250 bp) in 2.144 CPU sec, 2.181 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287742, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 276, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (297.41, 140.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 4, #reads = 1087630 mem = 536878211 peak = 897503232
[M::process] read 685924 sequences (100000260 bp) in 2.273 CPU sec, 2.360 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286549, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 276, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (297.05, 139.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686612 reads in 169.037 CPU sec, 5.437 real sec
[M::process] read 685606 sequences (100000068 bp) in 1.918 CPU sec, 1.940 real sec...
[M::mem_process_seqs] Processed 686388 reads in 107.682 CPU sec, 3.408 real sec
sorting block 5, #reads = 1088255 mem = 536878574 peak = 897503232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286091, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 278, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (298.06, 139.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685490 sequences (100000041 bp) in 2.708 CPU sec, 2.763 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286624, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 280, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (299.51, 140.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685572 sequences (100000020 bp) in 1.760 CPU sec, 1.760 real sec...
[M::mem_process_seqs] Processed 685924 reads in 161.437 CPU sec, 5.130 real sec
[M::mem_process_seqs] Processed 685606 reads in 106.052 CPU sec, 3.355 real sec
sorting block 6, #reads = 1087580 mem = 536880788 peak = 897503232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286188, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 281, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (301.68, 141.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685324 sequences (100000278 bp) in 2.091 CPU sec, 2.185 real sec...
[M::process] read 685502 sequences (100000218 bp) in 1.580 CPU sec, 1.587 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286202, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 282, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (300.73, 140.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685490 reads in 162.824 CPU sec, 5.197 real sec
[M::mem_process_seqs] Processed 685572 reads in 102.817 CPU sec, 3.280 real sec
sorting block 7, #reads = 1087822 mem = 536880471 peak = 897503232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286388, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 282, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (301.85, 141.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685198 sequences (100000134 bp) in 2.745 CPU sec, 2.797 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286444, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 280, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (300.97, 141.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685324 reads in 154.358 CPU sec, 4.886 real sec
sorting block 8, #reads = 1087217 mem = 536877746 peak = 897503232
[M::process] read 684690 sequences (100000019 bp) in 2.193 CPU sec, 2.208 real sec...
[M::mem_process_seqs] Processed 685502 reads in 102.485 CPU sec, 3.222 real sec
sorting block 9, #reads = 1087252 mem = 536877119 peak = 897503232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285824, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 283, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (303.61, 142.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (587, 836, 1953)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4685)
[M::mem_pestat] mean and std.dev: (1085.00, 788.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6051)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 684974 sequences (100000190 bp) in 2.505 CPU sec, 2.558 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285935, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 285, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (304.15, 141.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685198 reads in 154.427 CPU sec, 4.901 real sec
[M::mem_process_seqs] Processed 684690 reads in 101.042 CPU sec, 3.181 real sec
[M::process] read 684194 sequences (100000032 bp) in 2.388 CPU sec, 2.454 real sec...
sorting block 10, #reads = 1087009 mem = 536877432 peak = 897503232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285681, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 284, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (303.33, 141.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684028 sequences (100000009 bp) in 2.554 CPU sec, 2.619 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286271, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 286, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (305.51, 141.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684974 reads in 153.666 CPU sec, 4.907 real sec
[M::mem_process_seqs] Processed 684194 reads in 104.018 CPU sec, 3.276 real sec
[M::process] read 683684 sequences (100000033 bp) in 2.195 CPU sec, 2.262 real sec...
sorting block 11, #reads = 1086598 mem = 536884892 peak = 897503232
[M::process] read 683510 sequences (100000201 bp) in 2.448 CPU sec, 2.505 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285438, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 286, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (303.69, 138.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683684 reads in 84.647 CPU sec, 2.655 real sec
[M::process] read 683616 sequences (100000216 bp) in 2.277 CPU sec, 2.304 real sec...
sorting block 12, #reads = 1086300 mem = 536877493 peak = 897503232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285820, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 286, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (304.28, 139.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285558, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 287, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (304.82, 139.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684028 reads in 237.747 CPU sec, 7.521 real sec
[M::mem_process_seqs] Processed 683510 reads in 120.628 CPU sec, 3.817 real sec
[M::process] read 684360 sequences (100000187 bp) in 2.578 CPU sec, 2.609 real sec...
sorting block 13, #reads = 1085707 mem = 536879929 peak = 897503232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285432, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 285, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (304.29, 139.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683928 sequences (100000256 bp) in 2.533 CPU sec, 2.611 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286117, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 284, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (302.69, 139.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683616 reads in 150.016 CPU sec, 4.758 real sec
[M::mem_process_seqs] Processed 684360 reads in 99.127 CPU sec, 3.118 real sec
sorting block 14, #reads = 1085287 mem = 536890054 peak = 897503232
[M::process] read 684308 sequences (100000155 bp) in 2.367 CPU sec, 2.398 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285298, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 282, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (299.98, 136.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 977)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684066 sequences (100000028 bp) in 2.520 CPU sec, 2.579 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285698, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 281, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778)
[M::mem_pestat] mean and std.dev: (298.99, 136.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683928 reads in 151.264 CPU sec, 4.810 real sec
sorting block 15, #reads = 1084752 mem = 536879688 peak = 897503232
[M::mem_process_seqs] Processed 684308 reads in 101.415 CPU sec, 3.210 real sec
[M::process] read 683956 sequences (100000281 bp) in 2.306 CPU sec, 2.324 real sec...
sorting block 16, #reads = 1084274 mem = 536879948 peak = 897503232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285225, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 282, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (299.61, 136.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 977)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683536 sequences (100000035 bp) in 2.557 CPU sec, 2.604 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285699, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 283, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (301.17, 137.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684066 reads in 155.315 CPU sec, 4.930 real sec
[M::mem_process_seqs] Processed 683956 reads in 103.223 CPU sec, 3.250 real sec
[M::process] read 683768 sequences (100000097 bp) in 2.253 CPU sec, 2.274 real sec...
sorting block 17, #reads = 1083458 mem = 536882652 peak = 897503232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285305, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 287, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (305.59, 140.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683898 sequences (100000182 bp) in 2.534 CPU sec, 2.587 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285670, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 286, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (305.02, 141.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683536 reads in 151.616 CPU sec, 4.805 real sec
[M::mem_process_seqs] Processed 683768 reads in 101.867 CPU sec, 3.200 real sec
[M::process] read 683840 sequences (100000065 bp) in 2.355 CPU sec, 2.376 real sec...
sorting block 18, #reads = 1083096 mem = 536879974 peak = 897503232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285467, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 284, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (304.31, 141.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688554 sequences (100000270 bp) in 2.506 CPU sec, 2.558 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285833, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 287, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (306.89, 143.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683898 reads in 151.935 CPU sec, 4.808 real sec
[M::mem_process_seqs] Processed 683840 reads in 100.799 CPU sec, 3.165 real sec
[M::process] read 689268 sequences (100000258 bp) in 2.272 CPU sec, 2.291 real sec...
sorting block 19, #reads = 1083461 mem = 536878643 peak = 897503232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287092, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 264, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765)
[M::mem_pestat] mean and std.dev: (287.30, 137.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688094 sequences (100000058 bp) in 2.481 CPU sec, 2.526 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286790, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 261, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (283.66, 135.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688554 reads in 143.194 CPU sec, 4.534 real sec
sorting block 20, #reads = 1082327 mem = 536877506 peak = 897503232
[M::mem_process_seqs] Processed 689268 reads in 94.091 CPU sec, 2.956 real sec
[M::process] read 687782 sequences (100000239 bp) in 2.350 CPU sec, 2.375 real sec...
sorting block 21, #reads = 1089208 mem = 536879638 peak = 897503232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286542, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 266, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (288.13, 136.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687258 sequences (100000189 bp) in 2.588 CPU sec, 2.631 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286046, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 269, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (290.88, 137.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 978)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688094 reads in 140.464 CPU sec, 4.446 real sec
[M::mem_process_seqs] Processed 687782 reads in 93.530 CPU sec, 2.940 real sec
[M::process] read 687352 sequences (100000026 bp) in 2.400 CPU sec, 2.440 real sec...
sorting block 22, #reads = 1089270 mem = 536877819 peak = 897503232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286568, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 270, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (290.59, 136.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 964)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687310 sequences (100000016 bp) in 2.451 CPU sec, 2.495 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286511, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 272, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (292.42, 137.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687258 reads in 138.305 CPU sec, 4.398 real sec
[M::mem_process_seqs] Processed 687352 reads in 92.512 CPU sec, 2.913 real sec
sorting block 23, #reads = 1089022 mem = 536881082 peak = 924954624
[M::process] read 686660 sequences (100000267 bp) in 2.607 CPU sec, 2.672 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286523, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 271, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (292.71, 138.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686618 sequences (100000183 bp) in 2.531 CPU sec, 2.589 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286456, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 274, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (294.69, 138.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 982)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687310 reads in 135.890 CPU sec, 4.311 real sec
[M::mem_process_seqs] Processed 686660 reads in 90.955 CPU sec, 2.866 real sec
sorting block 24, #reads = 1089332 mem = 536881240 peak = 924954624
[M::process] read 686148 sequences (100000248 bp) in 2.650 CPU sec, 2.722 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286368, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 275, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (296.52, 140.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 25, #reads = 1089215 mem = 536883281 peak = 924954624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286595, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 276, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (297.16, 140.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686560 sequences (100000036 bp) in 2.375 CPU sec, 2.400 real sec...
[M::mem_process_seqs] Processed 686618 reads in 136.978 CPU sec, 4.347 real sec
[M::mem_process_seqs] Processed 686148 reads in 92.374 CPU sec, 2.905 real sec
sorting block 26, #reads = 1088222 mem = 536877969 peak = 924954624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286170, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 275, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (296.26, 139.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686036 sequences (100000088 bp) in 2.723 CPU sec, 2.801 real sec...
[M::mem_process_seqs] Processed 686560 reads in 79.306 CPU sec, 2.592 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286260, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 277, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (297.34, 138.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685992 sequences (100000177 bp) in 2.625 CPU sec, 2.714 real sec...
[M::mem_process_seqs] Processed 686036 reads in 77.846 CPU sec, 2.503 real sec
sorting block 27, #reads = 1087418 mem = 536880466 peak = 924954624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285892, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 279, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (298.13, 139.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 989)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685760 sequences (100000060 bp) in 2.331 CPU sec, 2.388 real sec...
[M::mem_process_seqs] Processed 685992 reads in 77.492 CPU sec, 2.498 real sec
sorting block 28, #reads = 1087384 mem = 536879307 peak = 924954624
[M::process] read 685574 sequences (100000097 bp) in 2.035 CPU sec, 2.106 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286499, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 279, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (298.82, 139.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685414 sequences (100000159 bp) in 1.500 CPU sec, 1.496 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286379, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 280, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (299.22, 139.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685760 reads in 137.996 CPU sec, 4.330 real sec
[M::mem_process_seqs] Processed 685574 reads in 92.197 CPU sec, 2.895 real sec
[M::process] read 685346 sequences (100000281 bp) in 1.659 CPU sec, 1.674 real sec...
sorting block 29, #reads = 1087436 mem = 536877582 peak = 924954624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286262, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 281, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (300.78, 140.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685052 sequences (100000225 bp) in 2.226 CPU sec, 2.358 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286647, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 281, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (301.26, 141.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685414 reads in 139.017 CPU sec, 4.412 real sec
[M::process] read 685296 sequences (100000072 bp) in 1.605 CPU sec, 1.611 real sec...
[M::mem_process_seqs] Processed 685346 reads in 92.920 CPU sec, 2.932 real sec
sorting block 30, #reads = 1087447 mem = 536882133 peak = 924954624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285709, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 283, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (301.97, 140.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685038 sequences (100000013 bp) in 2.475 CPU sec, 2.525 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285933, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 281, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (301.13, 141.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685052 reads in 139.844 CPU sec, 4.405 real sec
[M::process] read 684912 sequences (100000268 bp) in 1.986 CPU sec, 1.994 real sec...
[M::mem_process_seqs] Processed 685296 reads in 93.514 CPU sec, 2.940 real sec
sorting block 31, #reads = 1086964 mem = 536879102 peak = 924954624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286275, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 282, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (302.06, 140.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684656 sequences (100000110 bp) in 2.362 CPU sec, 2.415 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285768, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 282, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (301.23, 140.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685038 reads in 136.905 CPU sec, 4.333 real sec
sorting block 32, #reads = 1086332 mem = 536880577 peak = 943591424
[M::mem_process_seqs] Processed 684912 reads in 91.211 CPU sec, 2.867 real sec
[M::process] read 684640 sequences (100000287 bp) in 2.295 CPU sec, 2.319 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286018, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 284, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (302.90, 140.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 33, #reads = 1086025 mem = 536878345 peak = 974950400
[M::process] read 684572 sequences (100000172 bp) in 3.072 CPU sec, 3.267 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285637, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 282, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (301.09, 139.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684656 reads in 139.489 CPU sec, 4.431 real sec
[M::mem_process_seqs] Processed 684640 reads in 93.934 CPU sec, 2.960 real sec
sorting block 34, #reads = 1086081 mem = 536877540 peak = 974950400
[M::process] read 684680 sequences (100000211 bp) in 2.652 CPU sec, 2.718 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285758, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 281, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (300.94, 139.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684952 sequences (100000203 bp) in 2.187 CPU sec, 2.190 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286109, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 280, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (299.99, 139.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684572 reads in 139.216 CPU sec, 4.399 real sec
[M::mem_process_seqs] Processed 684680 reads in 92.789 CPU sec, 2.910 real sec
sorting block 35, #reads = 1085647 mem = 536877908 peak = 974950400
[M::process] read 684646 sequences (100000005 bp) in 2.588 CPU sec, 2.644 real sec...
[M::process] read 684268 sequences (100000016 bp) in 2.276 CPU sec, 2.300 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285900, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 279, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (298.68, 138.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684646 reads in 77.897 CPU sec, 2.448 real sec
sorting block 36, #reads = 1086207 mem = 536879908 peak = 974950400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285861, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 279, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (299.49, 139.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285503, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 280, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (298.14, 136.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 972)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684380 sequences (100000104 bp) in 2.445 CPU sec, 2.475 real sec...
[M::mem_process_seqs] Processed 684952 reads in 216.038 CPU sec, 6.827 real sec
[M::mem_process_seqs] Processed 684268 reads in 109.361 CPU sec, 3.450 real sec
sorting block 37, #reads = 1086593 mem = 536876387 peak = 974950400
[M::process] read 684262 sequences (100000043 bp) in 2.502 CPU sec, 2.557 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285692, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 282, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (300.65, 138.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684312 sequences (100000211 bp) in 2.244 CPU sec, 2.277 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285772, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 281, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (300.76, 139.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684380 reads in 136.829 CPU sec, 4.332 real sec
[M::mem_process_seqs] Processed 684262 reads in 91.216 CPU sec, 2.865 real sec
sorting block 38, #reads = 1085530 mem = 536877940 peak = 974950400
[M::process] read 683980 sequences (100000129 bp) in 2.639 CPU sec, 2.690 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285560, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 282, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (301.64, 140.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684042 sequences (100000027 bp) in 2.230 CPU sec, 2.250 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286012, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 284, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (304.41, 141.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684312 reads in 140.160 CPU sec, 4.441 real sec
sorting block 39, #reads = 1085712 mem = 536881967 peak = 974950400
[M::mem_process_seqs] Processed 683980 reads in 93.688 CPU sec, 2.944 real sec
[M::process] read 685392 sequences (100000022 bp) in 2.609 CPU sec, 2.676 real sec...
sorting block 40, #reads = 1086164 mem = 536879844 peak = 974950400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285872, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 285, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (304.91, 142.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690098 sequences (100000218 bp) in 2.258 CPU sec, 2.284 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285995, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 279, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (299.49, 141.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684042 reads in 141.457 CPU sec, 4.480 real sec
[M::mem_process_seqs] Processed 685392 reads in 96.738 CPU sec, 3.043 real sec
sorting block 41, #reads = 1084614 mem = 536879912 peak = 974950400
[M::process] read 688948 sequences (100000196 bp) in 2.683 CPU sec, 2.737 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287578, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 257, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (278.79, 131.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687414 sequences (100000177 bp) in 2.055 CPU sec, 2.074 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287105, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 262, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (283.89, 134.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 690098 reads in 140.101 CPU sec, 4.433 real sec
[M::mem_process_seqs] Processed 688948 reads in 91.645 CPU sec, 2.875 real sec
sorting block 42, #reads = 1087477 mem = 536878920 peak = 974950400
[M::process] read 687156 sequences (100000010 bp) in 1.981 CPU sec, 2.032 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287222, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 269, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771)
[M::mem_pestat] mean and std.dev: (290.15, 136.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 967)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686916 sequences (100000096 bp) in 2.248 CPU sec, 2.298 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286729, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 271, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (291.47, 136.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 968)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687414 reads in 140.672 CPU sec, 4.454 real sec
[M::mem_process_seqs] Processed 687156 reads in 92.428 CPU sec, 2.908 real sec
sorting block 43, #reads = 1089122 mem = 536877676 peak = 974950400
[M::process] read 686652 sequences (100000058 bp) in 2.180 CPU sec, 2.197 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286538, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 273, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (292.97, 137.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 44, #reads = 1088079 mem = 536878330 peak = 974950400
[M::process] read 686448 sequences (100000085 bp) in 1.907 CPU sec, 1.961 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286571, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 274, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (295.21, 139.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 982)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686074 sequences (100000001 bp) in 1.440 CPU sec, 1.440 real sec...
[M::mem_process_seqs] Processed 686916 reads in 135.351 CPU sec, 4.298 real sec
[M::mem_process_seqs] Processed 686652 reads in 90.390 CPU sec, 2.859 real sec
sorting block 45, #reads = 1087622 mem = 536878612 peak = 974950400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286033, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 275, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (296.22, 139.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685978 sequences (100000114 bp) in 2.138 CPU sec, 2.187 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286278, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 278, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (298.53, 140.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686066 sequences (100000037 bp) in 1.520 CPU sec, 1.526 real sec...
[M::mem_process_seqs] Processed 686448 reads in 134.420 CPU sec, 4.253 real sec
[M::mem_process_seqs] Processed 686074 reads in 90.041 CPU sec, 2.845 real sec
sorting block 46, #reads = 1087598 mem = 536880947 peak = 974950400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286002, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 279, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (299.67, 141.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685656 sequences (100000155 bp) in 2.278 CPU sec, 2.304 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286258, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 278, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (300.09, 142.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685978 reads in 137.507 CPU sec, 4.350 real sec
[M::mem_process_seqs] Processed 686066 reads in 91.771 CPU sec, 2.876 real sec
[M::process] read 685510 sequences (100000030 bp) in 2.233 CPU sec, 2.250 real sec...
sorting block 47, #reads = 1086555 mem = 536880126 peak = 995336192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286592, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 280, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (300.77, 142.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685184 sequences (100000098 bp) in 2.226 CPU sec, 2.266 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286685, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 281, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (302.30, 143.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685656 reads in 136.843 CPU sec, 4.333 real sec
sorting block 48, #reads = 1088791 mem = 536880935 peak = 995336192
[M::mem_process_seqs] Processed 685510 reads in 91.373 CPU sec, 2.869 real sec
[M::process] read 684986 sequences (100000053 bp) in 2.035 CPU sec, 2.038 real sec...
sorting block 49, #reads = 1087463 mem = 536883848 peak = 999190528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285830, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 284, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (304.22, 143.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686558 sequences (100000122 bp) in 2.278 CPU sec, 2.331 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286380, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 283, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (304.11, 143.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685184 reads in 138.725 CPU sec, 4.389 real sec
[M::mem_process_seqs] Processed 684986 reads in 92.373 CPU sec, 2.892 real sec
[M::process] read 685386 sequences (100000064 bp) in 2.125 CPU sec, 2.140 real sec...
sorting block 50, #reads = 1087810 mem = 536880259 peak = 999190528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286504, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 274, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (295.84, 140.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684406 sequences (100000025 bp) in 2.349 CPU sec, 2.396 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286184, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 281, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (302.27, 143.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686558 reads in 137.908 CPU sec, 4.371 real sec
[M::process] read 684584 sequences (100000014 bp) in 1.638 CPU sec, 1.656 real sec...
[M::mem_process_seqs] Processed 685386 reads in 91.573 CPU sec, 2.872 real sec
sorting block 51, #reads = 1087648 mem = 536878260 peak = 999190528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285697, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 284, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (304.95, 143.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684426 sequences (100000018 bp) in 2.040 CPU sec, 2.102 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285778, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 283, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (302.97, 141.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684244 sequences (100000074 bp) in 1.610 CPU sec, 1.601 real sec...
[M::mem_process_seqs] Processed 684406 reads in 136.296 CPU sec, 4.322 real sec
[M::mem_process_seqs] Processed 684584 reads in 90.172 CPU sec, 2.845 real sec
sorting block 52, #reads = 1087889 mem = 536877735 peak = 999190528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285991, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 283, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (302.96, 141.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684192 sequences (100000003 bp) in 2.732 CPU sec, 2.782 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285848, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 285, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (305.30, 143.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684426 reads in 135.803 CPU sec, 4.297 real sec
sorting block 53, #reads = 1087427 mem = 536880643 peak = 999190528
[M::mem_process_seqs] Processed 684244 reads in 89.560 CPU sec, 2.814 real sec
[M::process] read 684112 sequences (100000248 bp) in 2.373 CPU sec, 2.427 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285862, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 284, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (303.73, 141.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 54, #reads = 1086880 mem = 536881378 peak = 1018675200
[M::process] read 684576 sequences (100000043 bp) in 2.141 CPU sec, 2.198 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286227, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 282, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (302.03, 140.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684192 reads in 141.053 CPU sec, 4.476 real sec
[M::process] read 684576 sequences (100000034 bp) in 1.720 CPU sec, 1.735 real sec...
[M::mem_process_seqs] Processed 684112 reads in 93.448 CPU sec, 2.932 real sec
sorting block 55, #reads = 1086596 mem = 536880618 peak = 1018675200
[M::process] read 684970 sequences (100000161 bp) in 2.408 CPU sec, 2.479 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285908, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 280, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (300.36, 140.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684576 reads in 79.429 CPU sec, 2.492 real sec
[M::process] read 684906 sequences (100000063 bp) in 2.283 CPU sec, 2.297 real sec...
sorting block 56, #reads = 1086595 mem = 536879477 peak = 1018675200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285918, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 281, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (301.66, 141.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286527, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 279, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (299.98, 140.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684576 reads in 219.204 CPU sec, 6.925 real sec
[M::mem_process_seqs] Processed 684970 reads in 111.457 CPU sec, 3.518 real sec
[M::process] read 684588 sequences (100000291 bp) in 2.535 CPU sec, 2.572 real sec...
sorting block 57, #reads = 1086058 mem = 536876808 peak = 1018675200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285813, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 278, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (298.98, 140.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684286 sequences (100000262 bp) in 2.241 CPU sec, 2.319 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286252, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 282, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (301.73, 141.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684906 reads in 142.600 CPU sec, 4.524 real sec
[M::mem_process_seqs] Processed 684588 reads in 94.187 CPU sec, 2.957 real sec
[M::process] read 684032 sequences (100000159 bp) in 2.046 CPU sec, 2.083 real sec...
sorting block 58, #reads = 1085817 mem = 536878422 peak = 1018675200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285559, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 281, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (301.07, 139.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683952 sequences (100000197 bp) in 2.838 CPU sec, 2.903 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285611, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 284, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (303.41, 140.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684286 reads in 141.818 CPU sec, 4.491 real sec
[M::mem_process_seqs] Processed 684032 reads in 95.499 CPU sec, 3.004 real sec
sorting block 59, #reads = 1085824 mem = 536877784 peak = 1018675200
[M::process] read 684084 sequences (100000024 bp) in 2.754 CPU sec, 2.795 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285527, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 285, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (304.74, 141.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684172 sequences (100000088 bp) in 2.323 CPU sec, 2.356 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285910, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 286, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (305.75, 142.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683952 reads in 143.009 CPU sec, 4.528 real sec
sorting block 60, #reads = 1084700 mem = 536878806 peak = 1018675200
[M::mem_process_seqs] Processed 684084 reads in 95.817 CPU sec, 3.011 real sec
[M::process] read 685438 sequences (100000136 bp) in 2.101 CPU sec, 2.135 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286201, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 286, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (305.79, 143.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 61, #reads = 1084876 mem = 536879929 peak = 1038049280
[M::process] read 690054 sequences (100000061 bp) in 2.503 CPU sec, 2.556 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286194, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 281, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (302.24, 143.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684172 reads in 152.617 CPU sec, 4.833 real sec
[M::process] read 689188 sequences (100000088 bp) in 1.577 CPU sec, 1.584 real sec...
[M::mem_process_seqs] Processed 685438 reads in 107.939 CPU sec, 3.402 real sec
sorting block 62, #reads = 1084949 mem = 536885147 peak = 1038049280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287643, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 256, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (279.67, 133.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687162 sequences (100000107 bp) in 2.252 CPU sec, 2.321 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287269, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 258, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (281.53, 134.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 934)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 690054 reads in 139.696 CPU sec, 4.410 real sec
[M::process] read 686748 sequences (100000199 bp) in 2.309 CPU sec, 2.320 real sec...
[M::mem_process_seqs] Processed 689188 reads in 94.083 CPU sec, 2.950 real sec
sorting block 63, #reads = 1086040 mem = 536879771 peak = 1038049280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286438, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 269, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (292.30, 139.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686768 sequences (100000169 bp) in 2.773 CPU sec, 2.839 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286252, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 272, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (294.55, 139.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 981)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687162 reads in 141.288 CPU sec, 4.490 real sec
sorting block 64, #reads = 1089983 mem = 536883070 peak = 1038049280
[M::process] read 686360 sequences (100000064 bp) in 2.334 CPU sec, 2.415 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286114, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 275, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (297.10, 142.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686768 reads in 80.774 CPU sec, 2.549 real sec
[M::process] read 685956 sequences (100000163 bp) in 1.757 CPU sec, 1.773 real sec...
[M::mem_process_seqs] Processed 686748 reads in 198.800 CPU sec, 6.266 real sec
sorting block 65, #reads = 1088720 mem = 536877671 peak = 1038049280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286617, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 276, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (298.01, 141.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685924 sequences (100000053 bp) in 2.647 CPU sec, 2.722 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285792, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 278, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (299.63, 142.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686360 reads in 160.549 CPU sec, 5.065 real sec
[M::mem_process_seqs] Processed 685956 reads in 96.157 CPU sec, 3.024 real sec
sorting block 66, #reads = 1088102 mem = 536885613 peak = 1038049280
[M::process] read 685288 sequences (100000218 bp) in 2.530 CPU sec, 2.606 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286040, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 278, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (300.03, 142.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685542 sequences (100000128 bp) in 2.711 CPU sec, 2.779 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286183, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 280, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (301.55, 142.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685924 reads in 143.858 CPU sec, 4.571 real sec
sorting block 67, #reads = 1088527 mem = 536879706 peak = 1038049280
[M::mem_process_seqs] Processed 685288 reads in 95.874 CPU sec, 3.017 real sec
[M::process] read 685360 sequences (100000190 bp) in 2.694 CPU sec, 2.763 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285812, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 281, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (302.97, 144.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 68, #reads = 1088511 mem = 536878771 peak = 1038049280
[M::process] read 684982 sequences (100000050 bp) in 2.422 CPU sec, 2.478 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286510, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 283, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (304.14, 144.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685542 reads in 143.426 CPU sec, 4.552 real sec
[M::mem_process_seqs] Processed 685360 reads in 95.275 CPU sec, 3.001 real sec
[M::process] read 684744 sequences (100000260 bp) in 2.539 CPU sec, 2.598 real sec...
sorting block 69, #reads = 1087001 mem = 536879099 peak = 1038049280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286420, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 284, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (305.06, 144.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684942 sequences (100000116 bp) in 2.139 CPU sec, 2.193 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285380, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 284, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (305.09, 143.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684982 reads in 142.404 CPU sec, 4.515 real sec
[M::mem_process_seqs] Processed 684744 reads in 97.009 CPU sec, 3.056 real sec
[M::process] read 685942 sequences (100000196 bp) in 2.099 CPU sec, 2.157 real sec...
sorting block 70, #reads = 1087790 mem = 536878897 peak = 1038049280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285897, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 284, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (305.02, 144.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685284 sequences (100000091 bp) in 2.357 CPU sec, 2.414 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286517, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 277, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (299.92, 142.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684942 reads in 141.015 CPU sec, 4.475 real sec
[M::mem_process_seqs] Processed 685942 reads in 95.538 CPU sec, 3.006 real sec
[M::process] read 684140 sequences (100000017 bp) in 1.845 CPU sec, 1.879 real sec...
sorting block 71, #reads = 1086427 mem = 536877856 peak = 1038049280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286309, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 281, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (302.33, 143.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684378 sequences (100000059 bp) in 2.329 CPU sec, 2.417 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285797, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 286, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (306.23, 143.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685284 reads in 139.710 CPU sec, 4.422 real sec
[M::process] read 684070 sequences (100000022 bp) in 1.569 CPU sec, 1.574 real sec...
sorting block 72, #reads = 1087075 mem = 536879079 peak = 1038049280
[M::mem_process_seqs] Processed 684140 reads in 93.096 CPU sec, 2.938 real sec
sorting block 73, #reads = 1086662 mem = 536881798 peak = 1123192832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285475, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 286, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (306.65, 143.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683932 sequences (100000273 bp) in 3.009 CPU sec, 3.057 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285569, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 284, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (303.62, 140.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684378 reads in 143.795 CPU sec, 4.547 real sec
[M::mem_process_seqs] Processed 684070 reads in 96.502 CPU sec, 3.028 real sec
[M::process] read 683832 sequences (100000047 bp) in 2.505 CPU sec, 2.565 real sec...
sorting block 74, #reads = 1086474 mem = 536878831 peak = 1123192832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285502, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 284, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (302.92, 139.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684236 sequences (100000031 bp) in 2.384 CPU sec, 2.454 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285589, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 283, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (301.96, 139.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 989)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683932 reads in 140.866 CPU sec, 4.465 real sec
[M::mem_process_seqs] Processed 683832 reads in 96.136 CPU sec, 3.022 real sec
[M::process] read 684274 sequences (100000110 bp) in 2.412 CPU sec, 2.457 real sec...
sorting block 75, #reads = 1085240 mem = 536880277 peak = 1123192832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285562, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 281, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (300.23, 138.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 984)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684366 sequences (100000129 bp) in 2.655 CPU sec, 2.725 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285456, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 281, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (300.17, 138.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 984)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684236 reads in 141.819 CPU sec, 4.494 real sec
[M::mem_process_seqs] Processed 684274 reads in 95.073 CPU sec, 2.989 real sec
sorting block 76, #reads = 1086080 mem = 536876514 peak = 1123192832
[M::process] read 684280 sequences (100000028 bp) in 2.519 CPU sec, 2.573 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285594, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 281, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (300.72, 139.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684468 sequences (100000215 bp) in 2.501 CPU sec, 2.532 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285846, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 281, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (300.90, 139.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684366 reads in 140.362 CPU sec, 4.446 real sec
sorting block 77, #reads = 1085314 mem = 536879093 peak = 1123192832
[M::mem_process_seqs] Processed 684280 reads in 94.767 CPU sec, 2.979 real sec
[M::process] read 684408 sequences (100000260 bp) in 2.543 CPU sec, 2.592 real sec...
sorting block 78, #reads = 1085301 mem = 536876935 peak = 1123192832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286262, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 282, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (301.04, 139.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684056 sequences (100000253 bp) in 2.245 CPU sec, 2.292 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285812, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 283, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (302.58, 140.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684468 reads in 142.180 CPU sec, 4.502 real sec
[M::mem_process_seqs] Processed 684408 reads in 96.335 CPU sec, 3.026 real sec
[M::process] read 683942 sequences (100000172 bp) in 2.541 CPU sec, 2.592 real sec...
sorting block 79, #reads = 1086118 mem = 536882871 peak = 1123192832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285793, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 282, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (301.14, 139.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683894 sequences (100000120 bp) in 2.591 CPU sec, 2.646 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285536, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 283, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (303.09, 140.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684056 reads in 143.737 CPU sec, 4.554 real sec
[M::mem_process_seqs] Processed 683942 reads in 97.658 CPU sec, 3.074 real sec
sorting block 80, #reads = 1085181 mem = 536879382 peak = 1123192832
[M::process] read 683958 sequences (100000050 bp) in 2.544 CPU sec, 2.589 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285671, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 285, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (305.18, 141.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683904 sequences (100000031 bp) in 2.397 CPU sec, 2.430 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285688, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 286, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (306.25, 143.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683894 reads in 147.277 CPU sec, 4.657 real sec
[M::mem_process_seqs] Processed 683958 reads in 97.501 CPU sec, 3.065 real sec
sorting block 81, #reads = 1085399 mem = 536880147 peak = 1123192832
[M::process] read 683668 sequences (100000007 bp) in 1.873 CPU sec, 1.917 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285737, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 287, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (307.25, 144.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 82, #reads = 1084978 mem = 536883145 peak = 1123192832
[M::process] read 690742 sequences (100000243 bp) in 2.750 CPU sec, 2.796 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284874, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 287, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (306.77, 142.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683904 reads in 146.596 CPU sec, 4.631 real sec
[M::mem_process_seqs] Processed 683668 reads in 98.532 CPU sec, 3.095 real sec
[M::process] read 691798 sequences (100000135 bp) in 2.468 CPU sec, 2.494 real sec...
sorting block 83, #reads = 1084571 mem = 536876395 peak = 1123192832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287403, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 253, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (278.58, 134.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690796 sequences (100000076 bp) in 2.516 CPU sec, 2.585 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288295, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 248, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (274.45, 133.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 690742 reads in 167.583 CPU sec, 5.297 real sec
[M::process] read 690834 sequences (100000032 bp) in 2.118 CPU sec, 2.118 real sec...
[M::mem_process_seqs] Processed 691798 reads in 109.718 CPU sec, 3.450 real sec
sorting block 84, #reads = 1083546 mem = 536880047 peak = 1123192832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287928, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 255, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (281.19, 136.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690466 sequences (100000006 bp) in 2.910 CPU sec, 2.984 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288198, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 255, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (281.29, 136.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 690796 reads in 158.473 CPU sec, 4.998 real sec
[M::mem_process_seqs] Processed 690834 reads in 105.178 CPU sec, 3.299 real sec
[M::process] read 689866 sequences (100000290 bp) in 2.431 CPU sec, 2.448 real sec...
sorting block 85, #reads = 1086807 mem = 536877568 peak = 1123192832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287618, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 257, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (282.26, 136.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688784 sequences (100000031 bp) in 2.929 CPU sec, 2.967 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287626, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 261, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768)
[M::mem_pestat] mean and std.dev: (285.32, 137.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 965)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 690466 reads in 154.470 CPU sec, 4.887 real sec
sorting block 86, #reads = 1086908 mem = 536880921 peak = 1123192832
[M::mem_process_seqs] Processed 689866 reads in 101.678 CPU sec, 3.191 real sec
[M::process] read 688202 sequences (100000048 bp) in 2.266 CPU sec, 2.308 real sec...
sorting block 87, #reads = 1087862 mem = 536877757 peak = 1123192832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286823, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 265, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (289.36, 139.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688532 sequences (100000145 bp) in 2.425 CPU sec, 2.494 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287345, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 267, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (291.86, 140.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688784 reads in 151.004 CPU sec, 4.783 real sec
[M::process] read 687928 sequences (100000195 bp) in 1.991 CPU sec, 1.997 real sec...
[M::mem_process_seqs] Processed 688202 reads in 100.561 CPU sec, 3.164 real sec
sorting block 88, #reads = 1088256 mem = 536877440 peak = 1123192832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287272, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 268, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (291.50, 140.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687758 sequences (100000208 bp) in 2.470 CPU sec, 2.541 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287247, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 271, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (293.86, 141.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687708 sequences (100000136 bp) in 1.660 CPU sec, 1.653 real sec...
[M::mem_process_seqs] Processed 688532 reads in 150.535 CPU sec, 4.765 real sec
[M::mem_process_seqs] Processed 687928 reads in 100.310 CPU sec, 3.168 real sec
sorting block 89, #reads = 1085921 mem = 536877381 peak = 1123192832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286850, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 270, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (294.50, 141.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687410 sequences (100000232 bp) in 2.269 CPU sec, 2.330 real sec...
[M::process] read 687754 sequences (100000119 bp) in 1.690 CPU sec, 1.690 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286770, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 271, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (294.31, 140.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687758 reads in 151.540 CPU sec, 4.792 real sec
sorting block 90, #reads = 1087148 mem = 536879110 peak = 1123192832
[M::mem_process_seqs] Processed 687708 reads in 99.997 CPU sec, 3.168 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286952, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 273, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (295.89, 141.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687608 sequences (100000042 bp) in 2.448 CPU sec, 2.511 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286842, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 270, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (293.50, 140.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687264 sequences (100000004 bp) in 1.810 CPU sec, 1.806 real sec...
[M::mem_process_seqs] Processed 687410 reads in 150.146 CPU sec, 4.751 real sec
sorting block 91, #reads = 1087808 mem = 536878472 peak = 1123192832
[M::mem_process_seqs] Processed 687754 reads in 101.168 CPU sec, 3.203 real sec
sorting block 92, #reads = 1085634 mem = 536880852 peak = 1163579392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286606, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 271, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (295.23, 142.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687128 sequences (100000198 bp) in 2.466 CPU sec, 2.548 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286809, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 272, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (295.46, 141.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687330 sequences (100000003 bp) in 1.820 CPU sec, 1.817 real sec...
[M::mem_process_seqs] Processed 687608 reads in 152.543 CPU sec, 4.823 real sec
[M::mem_process_seqs] Processed 687264 reads in 102.148 CPU sec, 3.224 real sec
sorting block 93, #reads = 1085674 mem = 536880481 peak = 1163579392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286595, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 273, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (295.93, 141.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687132 sequences (100000192 bp) in 2.461 CPU sec, 2.537 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287381, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 273, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (296.10, 141.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687164 sequences (100000192 bp) in 1.775 CPU sec, 1.784 real sec...
[M::mem_process_seqs] Processed 687128 reads in 152.709 CPU sec, 4.832 real sec
[M::mem_process_seqs] Processed 687330 reads in 100.510 CPU sec, 3.177 real sec
sorting block 94, #reads = 1085177 mem = 536878289 peak = 1163579392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286655, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 273, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (296.66, 142.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686724 sequences (100000297 bp) in 2.862 CPU sec, 2.911 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287087, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 273, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (296.92, 142.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687132 reads in 147.366 CPU sec, 4.652 real sec
[M::mem_process_seqs] Processed 687164 reads in 99.402 CPU sec, 3.118 real sec
sorting block 95, #reads = 1085914 mem = 536878923 peak = 1163579392
[M::process] read 686590 sequences (100000192 bp) in 2.446 CPU sec, 2.471 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287413, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 276, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (298.56, 142.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 96, #reads = 1086205 mem = 536881533 peak = 1163579392
[M::process] read 686132 sequences (100000046 bp) in 2.846 CPU sec, 2.913 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286899, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 276, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (297.88, 141.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686724 reads in 155.348 CPU sec, 4.918 real sec
[M::mem_process_seqs] Processed 686590 reads in 104.729 CPU sec, 3.287 real sec
[M::process] read 685516 sequences (100000035 bp) in 2.570 CPU sec, 2.596 real sec...
sorting block 97, #reads = 1085961 mem = 536880300 peak = 1163579392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286666, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 276, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (297.54, 140.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685706 sequences (100000092 bp) in 2.761 CPU sec, 2.834 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286296, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 277, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (297.91, 139.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686132 reads in 162.927 CPU sec, 5.153 real sec
[M::mem_process_seqs] Processed 685516 reads in 108.248 CPU sec, 3.398 real sec
[M::process] read 685272 sequences (100000093 bp) in 2.411 CPU sec, 2.443 real sec...
sorting block 98, #reads = 1083191 mem = 536880609 peak = 1163579392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286766, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 277, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (297.51, 138.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685220 sequences (100000237 bp) in 2.574 CPU sec, 2.633 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286052, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 280, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (300.66, 141.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685706 reads in 156.815 CPU sec, 4.963 real sec
[M::process] read 684360 sequences (100000288 bp) in 1.789 CPU sec, 1.797 real sec...
sorting block 99, #reads = 1084398 mem = 536879912 peak = 1163579392
[M::mem_process_seqs] Processed 685272 reads in 104.705 CPU sec, 3.298 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286242, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 280, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (300.23, 140.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685260 sequences (100000100 bp) in 2.512 CPU sec, 2.577 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285862, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 284, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (303.97, 141.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685220 reads in 159.612 CPU sec, 5.038 real sec
[M::process] read 684888 sequences (100000262 bp) in 2.180 CPU sec, 2.174 real sec...
sorting block 100, #reads = 1083984 mem = 536879283 peak = 1163579392
[M::mem_process_seqs] Processed 684360 reads in 107.408 CPU sec, 3.382 real sec
sorting block 101, #reads = 1082870 mem = 536876133 peak = 1163579392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286532, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 280, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (301.10, 141.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685172 sequences (100000041 bp) in 2.827 CPU sec, 2.876 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286198, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 282, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (303.36, 143.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685260 reads in 158.649 CPU sec, 5.003 real sec
[M::process] read 692190 sequences (100000050 bp) in 2.421 CPU sec, 2.436 real sec...
[M::mem_process_seqs] Processed 684888 reads in 105.147 CPU sec, 3.300 real sec
sorting block 102, #reads = 1083875 mem = 536878570 peak = 1163579392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286310, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 280, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (302.03, 142.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697368 sequences (100000178 bp) in 2.797 CPU sec, 2.849 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288226, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 250, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (274.86, 133.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 695510 sequences (100000101 bp) in 1.860 CPU sec, 1.866 real sec...
[M::mem_process_seqs] Processed 685172 reads in 170.136 CPU sec, 5.382 real sec
[M::mem_process_seqs] Processed 692190 reads in 118.779 CPU sec, 3.748 real sec
sorting block 103, #reads = 1082013 mem = 536878987 peak = 1163579392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 290193, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 231, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (254.86, 121.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 694742 sequences (100000275 bp) in 2.885 CPU sec, 2.928 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 289037, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 238, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696)
[M::mem_pestat] mean and std.dev: (262.53, 126.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 873)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 693946 sequences (100000072 bp) in 2.339 CPU sec, 2.345 real sec...
[M::mem_process_seqs] Processed 697368 reads in 189.635 CPU sec, 5.982 real sec
sorting block 104, #reads = 1080501 mem = 536887736 peak = 1163579392
[M::mem_process_seqs] Processed 695510 reads in 123.194 CPU sec, 3.893 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 289728, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 692748 sequences (100000033 bp) in 2.595 CPU sec, 2.652 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (166, 240, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (265.08, 127.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 691288 sequences (100000242 bp) in 1.850 CPU sec, 1.855 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 289352, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 244, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (268.85, 129.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 694742 reads in 179.839 CPU sec, 5.692 real sec
sorting block 105, #reads = 1080240 mem = 536878201 peak = 1163579392
[M::mem_process_seqs] Processed 693946 reads in 200.626 CPU sec, 6.367 real sec
sorting block 106, #reads = 1082273 mem = 536878347 peak = 1190891520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 289098, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 248, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (271.96, 130.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 910)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 691502 sequences (100000129 bp) in 2.297 CPU sec, 2.359 real sec...
[M::process] read 690734 sequences (100000046 bp) in 1.899 CPU sec, 1.893 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288327, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 253, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (277.05, 132.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 692748 reads in 173.120 CPU sec, 5.486 real sec
[M::mem_process_seqs] Processed 691288 reads in 113.029 CPU sec, 3.588 real sec
sorting block 107, #reads = 1081698 mem = 536879918 peak = 1190891520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288899, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 253, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (277.43, 132.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690046 sequences (100000206 bp) in 2.524 CPU sec, 2.584 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287940, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 256, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (280.59, 134.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 691502 reads in 164.596 CPU sec, 5.190 real sec
[M::process] read 689104 sequences (100000178 bp) in 2.300 CPU sec, 2.300 real sec...
[M::mem_process_seqs] Processed 690734 reads in 107.600 CPU sec, 3.383 real sec
sorting block 108, #reads = 1082469 mem = 536879317 peak = 1190891520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287871, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 259, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (282.41, 134.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689070 sequences (100000092 bp) in 3.005 CPU sec, 3.064 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287292, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 262, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (284.57, 134.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 690046 reads in 165.150 CPU sec, 5.214 real sec
[M::process] read 688982 sequences (100000107 bp) in 2.314 CPU sec, 2.315 real sec...
sorting block 109, #reads = 1083989 mem = 536877064 peak = 1190891520
[M::mem_process_seqs] Processed 689104 reads in 108.568 CPU sec, 3.403 real sec
sorting block 110, #reads = 1081912 mem = 536882382 peak = 1190891520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287786, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 262, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (285.60, 136.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 963)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688154 sequences (100000044 bp) in 2.805 CPU sec, 2.851 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287395, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 264, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771)
[M::mem_pestat] mean and std.dev: (287.24, 136.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 968)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 689070 reads in 159.171 CPU sec, 5.025 real sec
[M::process] read 688436 sequences (100000025 bp) in 2.047 CPU sec, 2.052 real sec...
[M::mem_process_seqs] Processed 688982 reads in 106.165 CPU sec, 3.334 real sec
sorting block 111, #reads = 1083847 mem = 536878486 peak = 1190891520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287080, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 265, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (288.42, 136.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688624 sequences (100000272 bp) in 2.347 CPU sec, 2.389 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287435, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 265, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771)
[M::mem_pestat] mean and std.dev: (287.49, 136.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 968)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688056 sequences (100000285 bp) in 1.828 CPU sec, 1.832 real sec...
[M::mem_process_seqs] Processed 688154 reads in 156.316 CPU sec, 4.946 real sec
[M::mem_process_seqs] Processed 688436 reads in 101.811 CPU sec, 3.213 real sec
sorting block 112, #reads = 1083542 mem = 536882977 peak = 1190891520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287391, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 264, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (287.98, 137.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 972)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687588 sequences (100000090 bp) in 2.383 CPU sec, 2.438 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287326, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 267, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (291.43, 139.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687286 sequences (100000023 bp) in 1.750 CPU sec, 1.748 real sec...
[M::mem_process_seqs] Processed 688624 reads in 154.966 CPU sec, 4.905 real sec
sorting block 113, #reads = 1084193 mem = 536879185 peak = 1190891520
[M::mem_process_seqs] Processed 688056 reads in 104.490 CPU sec, 3.306 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286556, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 270, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (294.75, 142.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686662 sequences (100000083 bp) in 2.178 CPU sec, 2.230 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286963, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 272, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (296.89, 143.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686274 sequences (100000013 bp) in 1.720 CPU sec, 1.718 real sec...
[M::mem_process_seqs] Processed 687588 reads in 154.072 CPU sec, 4.883 real sec
sorting block 114, #reads = 1084455 mem = 536879570 peak = 1190891520
[M::mem_process_seqs] Processed 687286 reads in 100.891 CPU sec, 3.194 real sec
sorting block 115, #reads = 1084354 mem = 536879225 peak = 1190891520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286820, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 273, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (298.25, 144.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686154 sequences (100000103 bp) in 2.325 CPU sec, 2.385 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286573, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 273, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (297.71, 142.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685830 sequences (100000014 bp) in 1.719 CPU sec, 1.718 real sec...
[M::mem_process_seqs] Processed 686662 reads in 151.555 CPU sec, 4.810 real sec
[M::mem_process_seqs] Processed 686274 reads in 101.444 CPU sec, 3.197 real sec
sorting block 116, #reads = 1084334 mem = 536879136 peak = 1190891520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286787, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 275, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (299.02, 142.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685468 sequences (100000166 bp) in 2.563 CPU sec, 2.623 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286711, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 275, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (298.88, 142.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686154 reads in 149.175 CPU sec, 4.704 real sec
[M::process] read 685444 sequences (100000272 bp) in 2.225 CPU sec, 2.231 real sec...
[M::mem_process_seqs] Processed 685830 reads in 98.090 CPU sec, 3.075 real sec
sorting block 117, #reads = 1084462 mem = 536877000 peak = 1190891520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286597, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 278, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (302.13, 144.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685532 sequences (100000267 bp) in 2.646 CPU sec, 2.693 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286487, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 279, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (302.83, 145.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685468 reads in 150.261 CPU sec, 4.755 real sec
[M::process] read 685140 sequences (100000208 bp) in 1.669 CPU sec, 1.662 real sec...
sorting block 118, #reads = 1084098 mem = 536883691 peak = 1190891520
[M::mem_process_seqs] Processed 685444 reads in 100.686 CPU sec, 3.168 real sec
sorting block 119, #reads = 1083791 mem = 536879783 peak = 1190891520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286303, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 278, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (302.39, 145.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684928 sequences (100000001 bp) in 2.240 CPU sec, 2.300 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286147, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 280, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (304.37, 146.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689786 sequences (100000223 bp) in 2.120 CPU sec, 2.121 real sec...
[M::mem_process_seqs] Processed 685532 reads in 152.374 CPU sec, 4.813 real sec
[M::mem_process_seqs] Processed 685140 reads in 101.859 CPU sec, 3.212 real sec
sorting block 120, #reads = 1083008 mem = 536880576 peak = 1190891520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285687, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 283, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (306.35, 147.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 691680 sequences (100000097 bp) in 2.842 CPU sec, 2.904 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287403, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 258, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759)
[M::mem_pestat] mean and std.dev: (283.82, 137.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690226 sequences (100000183 bp) in 2.190 CPU sec, 2.190 real sec...
[M::mem_process_seqs] Processed 684928 reads in 174.803 CPU sec, 5.510 real sec
[M::mem_process_seqs] Processed 689786 reads in 123.861 CPU sec, 3.908 real sec
sorting block 121, #reads = 1080988 mem = 536877507 peak = 1190891520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288238, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 250, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (275.02, 133.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689488 sequences (100000226 bp) in 2.831 CPU sec, 2.877 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287798, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 257, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (281.17, 135.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 691680 reads in 152.800 CPU sec, 4.813 real sec
[M::process] read 689050 sequences (100000047 bp) in 2.255 CPU sec, 2.267 real sec...
[M::mem_process_seqs] Processed 690226 reads in 98.687 CPU sec, 3.101 real sec
sorting block 122, #reads = 1078594 mem = 536880937 peak = 1190891520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288005, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 261, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (284.69, 136.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688752 sequences (100000186 bp) in 2.749 CPU sec, 2.800 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287563, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 262, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (285.67, 136.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 959)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 689488 reads in 146.068 CPU sec, 4.617 real sec
sorting block 123, #reads = 1087392 mem = 536881464 peak = 1190891520
[M::mem_process_seqs] Processed 689050 reads in 97.785 CPU sec, 3.073 real sec
[M::process] read 688456 sequences (100000089 bp) in 2.390 CPU sec, 2.410 real sec...
sorting block 124, #reads = 1086947 mem = 536877906 peak = 1190891520
[M::process] read 687828 sequences (100000108 bp) in 2.654 CPU sec, 2.702 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286914, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 266, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (289.45, 139.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688456 reads in 80.952 CPU sec, 2.536 real sec
[M::process] read 687650 sequences (100000273 bp) in 2.513 CPU sec, 2.544 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287551, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 264, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771)
[M::mem_pestat] mean and std.dev: (287.92, 137.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 968)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287362, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 268, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (290.28, 137.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688752 reads in 228.438 CPU sec, 7.227 real sec
[M::mem_process_seqs] Processed 687828 reads in 116.120 CPU sec, 3.659 real sec
sorting block 125, #reads = 1087744 mem = 536878824 peak = 1190891520
[M::process] read 687250 sequences (100000173 bp) in 2.401 CPU sec, 2.444 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287022, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 268, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771)
[M::mem_pestat] mean and std.dev: (290.26, 137.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 967)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 126, #reads = 1087230 mem = 536880306 peak = 1190891520
[M::process] read 686986 sequences (100000029 bp) in 2.682 CPU sec, 2.739 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286689, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 269, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (291.96, 138.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687650 reads in 154.679 CPU sec, 4.902 real sec
[M::mem_process_seqs] Processed 687250 reads in 101.421 CPU sec, 3.188 real sec
[M::process] read 686772 sequences (100000212 bp) in 2.404 CPU sec, 2.453 real sec...
sorting block 127, #reads = 1086397 mem = 536880180 peak = 1190891520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286705, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 273, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (294.61, 139.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686706 sequences (100000234 bp) in 2.590 CPU sec, 2.657 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286555, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 273, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (295.64, 140.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686986 reads in 148.693 CPU sec, 4.714 real sec
[M::mem_process_seqs] Processed 686772 reads in 100.702 CPU sec, 3.167 real sec
[M::process] read 686712 sequences (100000009 bp) in 2.597 CPU sec, 2.650 real sec...
sorting block 128, #reads = 1086887 mem = 536879275 peak = 1190891520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286662, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 274, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (297.06, 141.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687954 sequences (100000285 bp) in 2.791 CPU sec, 2.866 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287054, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 274, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (296.09, 140.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686706 reads in 156.952 CPU sec, 4.977 real sec
[M::mem_process_seqs] Processed 686712 reads in 105.143 CPU sec, 3.310 real sec
sorting block 129, #reads = 1087417 mem = 536877054 peak = 1190891520
[M::process] read 687048 sequences (100000148 bp) in 2.525 CPU sec, 2.569 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286845, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 265, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (287.86, 136.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687684 sequences (100000035 bp) in 2.621 CPU sec, 2.692 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287350, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 270, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (293.08, 139.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687954 reads in 159.345 CPU sec, 5.061 real sec
sorting block 130, #reads = 1086519 mem = 536878059 peak = 1190891520
[M::mem_process_seqs] Processed 687048 reads in 106.539 CPU sec, 3.358 real sec
[M::process] read 687088 sequences (100000190 bp) in 2.701 CPU sec, 2.749 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286669, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 270, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (293.47, 140.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 131, #reads = 1086500 mem = 536877785 peak = 1190891520
[M::process] read 686268 sequences (100000077 bp) in 2.776 CPU sec, 2.848 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287028, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 271, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (295.57, 142.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687684 reads in 163.518 CPU sec, 5.177 real sec
[M::mem_process_seqs] Processed 687088 reads in 109.180 CPU sec, 3.435 real sec
[M::process] read 685830 sequences (100000075 bp) in 2.572 CPU sec, 2.619 real sec...
sorting block 132, #reads = 1086086 mem = 536879945 peak = 1190891520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286877, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 275, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (298.03, 142.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685946 sequences (100000260 bp) in 2.707 CPU sec, 2.779 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286096, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 277, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (300.03, 142.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686268 reads in 155.241 CPU sec, 4.919 real sec
[M::mem_process_seqs] Processed 685830 reads in 105.873 CPU sec, 3.328 real sec
[M::process] read 685970 sequences (100000173 bp) in 2.225 CPU sec, 2.264 real sec...
sorting block 133, #reads = 1085939 mem = 536877286 peak = 1190891520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286543, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 277, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (300.71, 144.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685530 sequences (100000061 bp) in 2.767 CPU sec, 2.826 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286596, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 276, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (299.72, 143.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685946 reads in 157.497 CPU sec, 4.988 real sec
[M::mem_process_seqs] Processed 685970 reads in 105.153 CPU sec, 3.309 real sec
sorting block 134, #reads = 1085124 mem = 536878132 peak = 1190891520
[M::process] read 685712 sequences (100000083 bp) in 2.525 CPU sec, 2.571 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286672, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 278, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (301.17, 143.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686536 sequences (100000111 bp) in 2.741 CPU sec, 2.813 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285606, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 276, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (300.19, 143.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685530 reads in 160.146 CPU sec, 5.072 real sec
sorting block 135, #reads = 1085332 mem = 536877488 peak = 1190891520
[M::mem_process_seqs] Processed 685712 reads in 108.478 CPU sec, 3.416 real sec
[M::process] read 685132 sequences (100000264 bp) in 2.492 CPU sec, 2.518 real sec...
sorting block 136, #reads = 1083122 mem = 536880223 peak = 1190891520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286976, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 271, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (294.97, 141.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685176 sequences (100000204 bp) in 2.791 CPU sec, 2.869 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286230, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 278, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (301.01, 143.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686536 reads in 159.065 CPU sec, 5.048 real sec
[M::mem_process_seqs] Processed 685132 reads in 106.397 CPU sec, 3.350 real sec
[M::process] read 685200 sequences (100000123 bp) in 2.500 CPU sec, 2.529 real sec...
sorting block 137, #reads = 1084481 mem = 536879531 peak = 1190891520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286140, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 278, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (300.87, 142.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685398 sequences (100000052 bp) in 2.745 CPU sec, 2.817 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286139, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 278, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (301.58, 143.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685176 reads in 158.742 CPU sec, 5.023 real sec
[M::mem_process_seqs] Processed 685200 reads in 107.535 CPU sec, 3.381 real sec
[M::process] read 685382 sequences (100000018 bp) in 2.348 CPU sec, 2.378 real sec...
sorting block 138, #reads = 1083708 mem = 536879907 peak = 1190891520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286293, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 276, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (299.70, 142.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685190 sequences (100000053 bp) in 2.842 CPU sec, 2.931 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285972, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 277, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (300.73, 143.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685398 reads in 176.588 CPU sec, 5.605 real sec
[M::process] read 685790 sequences (100000154 bp) in 2.464 CPU sec, 2.509 real sec...
[M::mem_process_seqs] Processed 685382 reads in 124.556 CPU sec, 3.927 real sec
sorting block 139, #reads = 1082665 mem = 536881827 peak = 1190891520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286066, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 278, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (301.45, 144.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 140, #reads = 1080938 mem = 536879276 peak = 1190891520
[M::process] read 698808 sequences (100000026 bp) in 3.108 CPU sec, 3.219 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285572, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 275, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (300.08, 144.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 695852 sequences (100000100 bp) in 2.159 CPU sec, 2.184 real sec...
[M::mem_process_seqs] Processed 685190 reads in 196.244 CPU sec, 6.234 real sec
[M::mem_process_seqs] Processed 685790 reads in 142.474 CPU sec, 4.510 real sec
sorting block 141, #reads = 1077421 mem = 536878204 peak = 1190891520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290371, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (250.79, 120.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 693194 sequences (100000182 bp) in 2.896 CPU sec, 2.997 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 290057, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 238, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (263.88, 128.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 884)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 691830 sequences (100000018 bp) in 2.472 CPU sec, 2.476 real sec...
[M::mem_process_seqs] Processed 698808 reads in 212.939 CPU sec, 6.758 real sec
[M::mem_process_seqs] Processed 695852 reads in 142.223 CPU sec, 4.510 real sec
sorting block 142, #reads = 1075793 mem = 536883545 peak = 1190891520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288737, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 246, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (271.60, 130.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 913)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 691492 sequences (100000144 bp) in 3.281 CPU sec, 3.377 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 288165, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 252, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (276.70, 133.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689792 sequences (100000265 bp) in 2.405 CPU sec, 2.409 real sec...
[M::mem_process_seqs] Processed 693194 reads in 201.448 CPU sec, 6.381 real sec
sorting block 143, #reads = 1080976 mem = 536882180 peak = 1190891520
[M::mem_process_seqs] Processed 691830 reads in 135.747 CPU sec, 4.293 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288477, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 254, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (279.56, 135.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689440 sequences (100000237 bp) in 3.113 CPU sec, 3.211 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 288198, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 260, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762)
[M::mem_pestat] mean and std.dev: (283.60, 135.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688922 sequences (100000173 bp) in 2.380 CPU sec, 2.375 real sec...
[M::mem_process_seqs] Processed 691492 reads in 193.140 CPU sec, 6.114 real sec
sorting block 144, #reads = 1080405 mem = 536880871 peak = 1190891520
[M::mem_process_seqs] Processed 689792 reads in 129.384 CPU sec, 4.096 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287944, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 262, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (286.41, 137.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 145, #reads = 1082172 mem = 536880068 peak = 1213059072
[M::process] read 688838 sequences (100000115 bp) in 3.340 CPU sec, 3.501 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 287925, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 265, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (288.82, 138.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 689440 reads in 192.704 CPU sec, 6.120 real sec
[M::process] read 687638 sequences (100000164 bp) in 2.363 CPU sec, 2.376 real sec...
[M::mem_process_seqs] Processed 688922 reads in 131.753 CPU sec, 4.160 real sec
sorting block 146, #reads = 1081125 mem = 536878491 peak = 1213059072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287681, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 266, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (290.68, 139.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687272 sequences (100000270 bp) in 2.814 CPU sec, 2.877 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287578, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 273, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (294.94, 140.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688838 reads in 180.204 CPU sec, 5.717 real sec
[M::process] read 688368 sequences (100000169 bp) in 2.349 CPU sec, 2.349 real sec...
sorting block 147, #reads = 1081773 mem = 536880907 peak = 1213059072
[M::mem_process_seqs] Processed 687638 reads in 119.795 CPU sec, 3.775 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286775, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 273, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (295.66, 140.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686370 sequences (100000001 bp) in 2.969 CPU sec, 3.034 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287333, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 266, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778)
[M::mem_pestat] mean and std.dev: (289.91, 139.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 977)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686238 sequences (100000123 bp) in 2.220 CPU sec, 2.227 real sec...
[M::mem_process_seqs] Processed 687272 reads in 170.958 CPU sec, 5.382 real sec
sorting block 148, #reads = 1080910 mem = 536880805 peak = 1213059072
[M::mem_process_seqs] Processed 688368 reads in 190.584 CPU sec, 6.020 real sec
sorting block 149, #reads = 1081761 mem = 536879899 peak = 1213059072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287267, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 276, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (298.18, 141.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686470 sequences (100000058 bp) in 2.754 CPU sec, 2.831 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287080, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 278, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (300.60, 143.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686112 sequences (100000258 bp) in 1.969 CPU sec, 1.985 real sec...
[M::mem_process_seqs] Processed 686370 reads in 168.552 CPU sec, 5.326 real sec
[M::mem_process_seqs] Processed 686238 reads in 112.787 CPU sec, 3.559 real sec
sorting block 150, #reads = 1082494 mem = 536878836 peak = 1213059072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286885, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 277, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (300.15, 143.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685672 sequences (100000227 bp) in 2.603 CPU sec, 2.662 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286664, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 276, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (298.78, 141.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685510 sequences (100000062 bp) in 1.850 CPU sec, 1.856 real sec...
[M::mem_process_seqs] Processed 686470 reads in 169.992 CPU sec, 5.385 real sec
[M::mem_process_seqs] Processed 686112 reads in 116.237 CPU sec, 3.684 real sec
sorting block 151, #reads = 1083118 mem = 536880188 peak = 1213059072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286502, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 276, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (298.37, 140.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685420 sequences (100000300 bp) in 2.723 CPU sec, 2.784 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286558, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 275, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (297.74, 140.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685680 sequences (100000254 bp) in 2.010 CPU sec, 2.014 real sec...
[M::mem_process_seqs] Processed 685672 reads in 171.738 CPU sec, 5.428 real sec
[M::mem_process_seqs] Processed 685510 reads in 115.655 CPU sec, 3.655 real sec
sorting block 152, #reads = 1082832 mem = 536878911 peak = 1213059072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286058, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 277, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (300.15, 142.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685084 sequences (100000022 bp) in 2.855 CPU sec, 2.918 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286354, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 276, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (298.96, 141.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685420 reads in 166.430 CPU sec, 5.258 real sec
[M::process] read 685430 sequences (100000052 bp) in 2.275 CPU sec, 2.282 real sec...
sorting block 153, #reads = 1082267 mem = 536875981 peak = 1213059072
[M::mem_process_seqs] Processed 685680 reads in 110.819 CPU sec, 3.485 real sec
sorting block 154, #reads = 1083376 mem = 536880645 peak = 1213059072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285878, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 277, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (299.36, 140.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684712 sequences (100000063 bp) in 2.696 CPU sec, 2.749 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286585, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 277, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (299.23, 141.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684832 sequences (100000026 bp) in 1.890 CPU sec, 1.895 real sec...
[M::mem_process_seqs] Processed 685084 reads in 161.543 CPU sec, 5.109 real sec
[M::mem_process_seqs] Processed 685430 reads in 109.173 CPU sec, 3.451 real sec
sorting block 155, #reads = 1083350 mem = 536877773 peak = 1213059072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285951, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 279, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (301.02, 141.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684620 sequences (100000242 bp) in 2.850 CPU sec, 2.924 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285799, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 279, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (302.13, 143.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684596 sequences (100000027 bp) in 1.810 CPU sec, 1.806 real sec...
[M::mem_process_seqs] Processed 684712 reads in 163.245 CPU sec, 5.163 real sec
[M::mem_process_seqs] Processed 684832 reads in 110.779 CPU sec, 3.505 real sec
sorting block 156, #reads = 1083648 mem = 536881556 peak = 1213059072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285686, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 280, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (302.61, 143.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (122, 214, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428)
[M::mem_pestat] mean and std.dev: (192.40, 67.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 530)
[M::mem_pestat] skip orientation RR
[M::process] read 684420 sequences (100000023 bp) in 2.925 CPU sec, 2.977 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286263, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 280, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (302.48, 143.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684620 reads in 162.124 CPU sec, 5.123 real sec
[M::process] read 684368 sequences (100000130 bp) in 2.362 CPU sec, 2.384 real sec...
[M::mem_process_seqs] Processed 684596 reads in 109.323 CPU sec, 3.436 real sec
sorting block 157, #reads = 1083403 mem = 536879370 peak = 1213059072
[M::process] read 684974 sequences (100000026 bp) in 3.021 CPU sec, 3.095 real sec...
[M::process] read 40534 sequences (5873625 bp) in 0.340 CPU sec, 0.349 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285782, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 282, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (304.56, 144.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684368 reads in 86.870 CPU sec, 2.763 real sec
sorting block 158, #reads = 1084642 mem = 536879790 peak = 1213059072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285845, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 282, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (304.84, 144.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286050, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 282, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (303.86, 144.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684420 reads in 246.665 CPU sec, 7.909 real sec
[M::mem_process_seqs] Processed 684974 reads in 126.905 CPU sec, 4.114 real sec
sorting block 159, #reads = 1084456 mem = 536880637 peak = 1213059072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 16785, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 267, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (291.05, 141.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 40534 reads in 7.232 CPU sec, 0.255 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 173745242
overall: 69928456192 mem 22734.115 user 497.206 sys 751.475 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_cfy-T1-TDNA-1_A23MHWWLT4_1.L005\tPU:L005\tSM:659_cfy-T1-TDNA-1_A23MHWWLT4_1\tLB:659_cfy-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_cfy-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_cfy-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 751.510 sec; CPU: 23231.324 sec
sorting block 160, #reads = 943074 mem = 467594803 peak = 1228582912
uniq header count 1
merging 161 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 9522691019
output reads: 174608963
bam_mem_sort: 161 calls 85.677 user 1.547 sys 87.461 real
bam_write: 161 calls 50.297 user 2.887 sys 57.414 real
execute: 1 calls 50.352 user 1.575 sys 824.640 real
merge_files: 1 calls 50.342 user 1.530 sys 72.405 real
parse_chunk: 2574 calls 140.064 user 32.679 sys 175.992 real
read_chunk: 6941 calls 71.523 user 47.818 sys 722.066 real
sort_block: 1 calls 49.738 user 47.382 sys 691.674 real
write_chunk: 4365 calls 50.007 user 1.153 sys 71.410 real
overall: 3237793792 mem 2829.470 user 106.385 sys 824.661 real
ls: cannot access '*.cram': No such file or directory