File Info

Filename
.command.err
Full Path
s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/8c/d9326ee4aa8d0833d596b47cc2ebe3/.command.err
Size
1.8 KB
Task
DAQ:FASTP (1029_IWL-T1-TDNA-1_A23MHWWLT4_1;L007)
Attempt

Content

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Detecting adapter sequence for read1...
>Illumina TruSeq Adapter Read 1
AGATCGGAAGAGCACACGTCTGAACTCCAGTCA

Detecting adapter sequence for read2...
>Illumina TruSeq Adapter Read 2
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT

Read1 before filtering:
total reads: 44088242
total bases: 6657324542
Q20 bases: 6453448803(96.9376%)
Q30 bases: 6176255993(92.7738%)
Q40 bases: 6176255993(92.7738%)

Read2 before filtering:
total reads: 44088242
total bases: 6657324542
Q20 bases: 6469466494(97.1782%)
Q30 bases: 6158081706(92.5008%)
Q40 bases: 6158081706(92.5008%)

Read1 after filtering:
total reads: 43757904
total bases: 5713172275
Q20 bases: 5621821326(98.401%)
Q30 bases: 5421647016(94.8973%)
Q40 bases: 5421647016(94.8973%)

Read2 after filtering:
total reads: 43757904
total bases: 5714585032
Q20 bases: 5617409749(98.2995%)
Q30 bases: 5413028484(94.723%)
Q40 bases: 5413028484(94.723%)

Filtering result:
reads passed filter: 87515808
reads failed due to low quality: 650768
reads failed due to too many N: 7512
reads failed due to too short: 2396
reads with adapter trimmed: 45021227
bases trimmed due to adapters: 1792884919

Duplication rate: 8.61204%

Insert size peak (evaluated by paired-end reads): 116

JSON report: 1029_IWL-T1-TDNA-1_A23MHWWLT4_1.fastp.json
HTML report: 1029_IWL-T1-TDNA-1_A23MHWWLT4_1.fastp.html

fastp --in1 1029_IWL-T1-TDNA-1_A23MHWWLT4_1_S7_L007_R1_001.fastq.gz --in2 1029_IWL-T1-TDNA-1_A23MHWWLT4_1_S7_L007_R2_001.fastq.gz --out1 1029_IWL-T1-TDNA-1_A23MHWWLT4_1/1029_IWL-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz --out2 1029_IWL-T1-TDNA-1_A23MHWWLT4_1/1029_IWL-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz --detect_adapter_for_pe --length_required 15 --json 1029_IWL-T1-TDNA-1_A23MHWWLT4_1.fastp.json --html 1029_IWL-T1-TDNA-1_A23MHWWLT4_1.fastp.html --thread 4 
fastp v1.0.1, time used: 372 seconds