.command.log - Workdir - DAQ:SENTIEON_BWAMEM (1136_3U6-T1-TDNA-1_A23MHWWLT4_1;L001;0002)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/64/5d8ac09fe1e53f8122987589bc81aa/.command.log
Size: 129.7 KB
Task: DAQ:SENTIEON_BWAMEM (1136_3U6-T1-TDNA-1_A23MHWWLT4_1;L001;0002)
Attempt
Content

  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/64/5d8ac09fe1e53f8122987589bc81aa/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/5f/f8555ba5e6b5b6e1c774cb97ce0bc4/1136_3U6-T1-TDNA-1_A23MHWWLT4_1/0002.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/5f/f8555ba5e6b5b6e1c774cb97ce0bc4/1136_3U6-T1-TDNA-1_A23MHWWLT4_1/0002.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/64/5d8ac09fe1e53f8122987589bc81aa/.command.run
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_3U6-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1699.176 CPU sec, 538.624 real sec
[M::process] read 685146 sequences (100000064 bp) in 0.963 CPU sec, 0.969 real sec...
[M::process] read 683196 sequences (100000112 bp) in 1.166 CPU sec, 1.239 real sec...
[M::process] read 682596 sequences (100000263 bp) in 1.168 CPU sec, 1.173 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283518, 4, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (112, 161, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1267)
[M::mem_pestat] mean and std.dev: (225.26, 204.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1652)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 314, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (331.16, 162.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (283, 441, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (373.15, 125.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681902 sequences (100000165 bp) in 1.129 CPU sec, 1.123 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282531, 3, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (291, 436, 1536)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4026)
[M::mem_pestat] mean and std.dev: (815.14, 611.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5271)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 327, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (341.82, 166.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (141, 181, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372)
[M::mem_pestat] mean and std.dev: (158.67, 35.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 449)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282432, 7, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 271, 788)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1952)
[M::mem_pestat] mean and std.dev: (550.43, 515.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2613)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 331, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (344.52, 166.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 369, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (350.28, 173.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685146 reads in 227.918 CPU sec, 7.314 real sec
[M::mem_process_seqs] Processed 683196 reads in 144.757 CPU sec, 4.688 real sec
[M::mem_process_seqs] Processed 682596 reads in 156.899 CPU sec, 5.100 real sec
sorting block 0, #reads = 1080434 mem = 536880166 peak = 601829376
[M::process] read 682664 sequences (100000153 bp) in 1.691 CPU sec, 1.861 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 282723, 2, 21)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 338, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (350.38, 168.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 304, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1091)
[M::mem_pestat] mean and std.dev: (365.95, 248.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1391)
[M::mem_pestat] skip orientation RR
[M::process] read 682346 sequences (100000105 bp) in 1.204 CPU sec, 1.221 real sec...
[M::process] read 682020 sequences (100000095 bp) in 1.090 CPU sec, 1.087 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 282331, 3, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (359, 584, 1456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3650)
[M::mem_pestat] mean and std.dev: (555.38, 405.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4747)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 331, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (345.43, 167.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 345, 570)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1314)
[M::mem_pestat] mean and std.dev: (347.35, 161.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1686)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681902 reads in 139.314 CPU sec, 4.437 real sec
sorting block 1, #reads = 1079197 mem = 536881783 peak = 940797952
[M::mem_process_seqs] Processed 682664 reads in 87.164 CPU sec, 2.764 real sec
sorting block 2, #reads = 1080556 mem = 536879255 peak = 965361664
[M::process] read 682720 sequences (100000093 bp) in 1.500 CPU sec, 1.586 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 281894, 3, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 416, 1629)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4545)
[M::mem_pestat] mean and std.dev: (652.30, 609.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6003)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 331, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (345.50, 166.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (297, 762, 1643)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4335)
[M::mem_pestat] mean and std.dev: (928.00, 701.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5681)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682470 sequences (100000093 bp) in 1.213 CPU sec, 1.236 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 281618, 11, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 330, 1411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3865)
[M::mem_pestat] mean and std.dev: (620.34, 601.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5092)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 330, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (344.41, 165.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (316, 575, 575)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093)
[M::mem_pestat] mean and std.dev: (422.80, 194.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1352)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 343, 789)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1903)
[M::mem_pestat] mean and std.dev: (477.79, 316.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2460)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 281983, 1, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (219, 258, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (258.88, 106.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 707)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 321, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (335.37, 159.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 300, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037)
[M::mem_pestat] mean and std.dev: (283.52, 134.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1318)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682346 reads in 207.052 CPU sec, 6.647 real sec
[M::mem_process_seqs] Processed 682020 reads in 151.924 CPU sec, 4.900 real sec
[M::mem_process_seqs] Processed 682720 reads in 164.499 CPU sec, 5.329 real sec
sorting block 3, #reads = 1079803 mem = 536880125 peak = 965361664
[M::process] read 681932 sequences (100000066 bp) in 1.905 CPU sec, 2.048 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 281516, 5, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (197, 213, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (5, 485)
[M::mem_pestat] mean and std.dev: (247.68, 76.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 581)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 321, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (335.50, 160.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 165, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (231.79, 137.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 4, #reads = 1079362 mem = 536880496 peak = 985071616
[M::process] read 681364 sequences (100000085 bp) in 1.395 CPU sec, 1.440 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 281628, 4, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (255, 416, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (341.79, 130.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 324, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (338.75, 161.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 409, 581)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1349)
[M::mem_pestat] mean and std.dev: (366.33, 199.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1733)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681088 sequences (100000109 bp) in 1.200 CPU sec, 1.192 real sec...
[M::mem_process_seqs] Processed 682470 reads in 148.667 CPU sec, 4.752 real sec
[M::mem_process_seqs] Processed 681932 reads in 92.027 CPU sec, 2.919 real sec
sorting block 5, #reads = 1077809 mem = 536876533 peak = 985071616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 282155, 3, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 223, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (217.58, 77.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 326, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (341.40, 162.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (289, 494, 1434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3724)
[M::mem_pestat] mean and std.dev: (642.93, 584.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4869)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683878 sequences (100000266 bp) in 1.688 CPU sec, 1.811 real sec...
[M::process] read 682852 sequences (100000053 bp) in 1.220 CPU sec, 1.221 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 281860, 10, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 263, 649)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1625)
[M::mem_pestat] mean and std.dev: (420.95, 381.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2113)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 328, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (343.29, 163.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1430, 1839, 2579)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4877)
[M::mem_pestat] mean and std.dev: (1742.56, 839.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6026)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 464, 958)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2434)
[M::mem_pestat] mean and std.dev: (607.95, 459.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3172)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681364 reads in 141.056 CPU sec, 4.518 real sec
[M::mem_process_seqs] Processed 681088 reads in 86.827 CPU sec, 2.776 real sec
sorting block 6, #reads = 1078942 mem = 536882621 peak = 985071616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 283044, 6, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 253, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (224.50, 67.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 755)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 319, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (335.65, 163.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (323, 491, 558)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (410.08, 173.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682184 sequences (100000058 bp) in 1.695 CPU sec, 1.845 real sec...
[M::process] read 681296 sequences (100000207 bp) in 1.220 CPU sec, 1.220 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282656, 1, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (352, 431, 696)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1384)
[M::mem_pestat] mean and std.dev: (493.03, 268.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1728)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 330, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (344.79, 168.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (307, 378, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (119, 589)
[M::mem_pestat] mean and std.dev: (347.00, 82.04)
[M::mem_pestat] low and high boundaries for proper pairs: (19, 683)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683878 reads in 138.609 CPU sec, 4.442 real sec
[M::mem_process_seqs] Processed 682852 reads in 85.661 CPU sec, 2.739 real sec
sorting block 7, #reads = 1079683 mem = 536878894 peak = 985071616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282838, 4, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (208, 355, 549)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1231)
[M::mem_pestat] mean and std.dev: (322.27, 148.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1572)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 335, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (349.28, 169.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 232, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734)
[M::mem_pestat] mean and std.dev: (259.82, 138.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 930)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682282 sequences (100000224 bp) in 1.971 CPU sec, 2.093 real sec...
[M::process] read 682222 sequences (100000285 bp) in 1.260 CPU sec, 1.261 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 281981, 4, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 261, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (283.53, 140.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 342, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (354.32, 170.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 263, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (5, 575)
[M::mem_pestat] mean and std.dev: (258.12, 63.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 689)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682184 reads in 139.168 CPU sec, 4.453 real sec
sorting block 8, #reads = 1081937 mem = 536881425 peak = 985071616
[M::mem_process_seqs] Processed 681296 reads in 86.237 CPU sec, 2.755 real sec
sorting block 9, #reads = 1080141 mem = 536877804 peak = 985071616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282339, 7, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 451, 961)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2579)
[M::mem_pestat] mean and std.dev: (484.38, 380.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3388)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 335, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (349.46, 169.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 186, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (286.33, 146.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682224 sequences (100000163 bp) in 1.785 CPU sec, 1.909 real sec...
[M::process] read 682956 sequences (100000212 bp) in 1.310 CPU sec, 1.316 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 282626, 2, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (246, 270, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (269.00, 90.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 332, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (347.11, 168.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (472, 801, 1426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3334)
[M::mem_pestat] mean and std.dev: (909.93, 683.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4288)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682282 reads in 141.352 CPU sec, 4.527 real sec
[M::mem_process_seqs] Processed 682222 reads in 88.163 CPU sec, 2.810 real sec
sorting block 10, #reads = 1080962 mem = 536881216 peak = 985071616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282063, 4, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (227, 244, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (281.44, 104.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 329, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (343.97, 166.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 312, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (277.78, 148.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682816 sequences (100000150 bp) in 2.108 CPU sec, 2.226 real sec...
[M::process] read 682032 sequences (100000020 bp) in 1.313 CPU sec, 1.324 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282417, 4, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 239, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (292.90, 124.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 323, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (339.44, 165.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 327, 564)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1246)
[M::mem_pestat] mean and std.dev: (305.43, 155.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1587)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682224 reads in 142.755 CPU sec, 4.559 real sec
[M::mem_process_seqs] Processed 682956 reads in 89.169 CPU sec, 2.843 real sec
sorting block 11, #reads = 1079167 mem = 536879025 peak = 985071616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282791, 4, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 329, 1099)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2927)
[M::mem_pestat] mean and std.dev: (609.00, 486.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3841)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 322, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (337.35, 163.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 322, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (324.63, 139.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681424 sequences (100000246 bp) in 1.991 CPU sec, 2.173 real sec...
[M::process] read 680970 sequences (100000034 bp) in 1.320 CPU sec, 1.320 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282815, 0, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 268, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595)
[M::mem_pestat] mean and std.dev: (266.70, 104.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 324, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (340.59, 164.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 263, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1072)
[M::mem_pestat] mean and std.dev: (350.86, 217.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1358)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682816 reads in 142.645 CPU sec, 4.560 real sec
[M::mem_process_seqs] Processed 682032 reads in 87.282 CPU sec, 2.782 real sec
sorting block 12, #reads = 1077915 mem = 536877930 peak = 985071616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282611, 3, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 194, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (263.88, 160.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 328, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (342.86, 163.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 333, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076)
[M::mem_pestat] mean and std.dev: (346.64, 159.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1348)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684268 sequences (100000134 bp) in 2.185 CPU sec, 2.347 real sec...
[M::process] read 682686 sequences (100000054 bp) in 1.340 CPU sec, 1.330 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 282438, 6, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 319, 642)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1564)
[M::mem_pestat] mean and std.dev: (471.55, 422.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2162)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 333, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (348.21, 167.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 273, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (282.43, 142.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1302)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681424 reads in 140.249 CPU sec, 4.476 real sec
sorting block 13, #reads = 1079931 mem = 536878871 peak = 996012032
[M::mem_process_seqs] Processed 680970 reads in 87.422 CPU sec, 2.784 real sec
sorting block 14, #reads = 1078841 mem = 536878214 peak = 1031655424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 283773, 3, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (101, 172, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1259)
[M::mem_pestat] mean and std.dev: (316.33, 279.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1645)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 320, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (336.36, 165.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 253, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684)
[M::mem_pestat] mean and std.dev: (267.59, 126.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681452 sequences (100000191 bp) in 1.934 CPU sec, 2.062 real sec...
[M::process] read 681042 sequences (100000069 bp) in 1.333 CPU sec, 1.335 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 282764, 9, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 249, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (318.00, 203.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 330, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (344.57, 167.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 329, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (284.89, 130.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1103)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684268 reads in 139.308 CPU sec, 4.456 real sec
[M::mem_process_seqs] Processed 682686 reads in 86.520 CPU sec, 2.760 real sec
sorting block 15, #reads = 1079848 mem = 536877144 peak = 1031655424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 282559, 4, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 301, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048)
[M::mem_pestat] mean and std.dev: (396.22, 311.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1642)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 338, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (351.63, 169.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 255, 919)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2345)
[M::mem_pestat] mean and std.dev: (544.56, 544.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3058)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682250 sequences (100000254 bp) in 1.995 CPU sec, 2.108 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282620, 4, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 333, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (328.67, 172.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1321)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 346, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (359.08, 173.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 642, 699)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1619)
[M::mem_pestat] mean and std.dev: (499.36, 318.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2079)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681824 sequences (100000050 bp) in 1.300 CPU sec, 1.302 real sec...
[M::mem_process_seqs] Processed 681452 reads in 136.084 CPU sec, 4.346 real sec
[M::mem_process_seqs] Processed 681042 reads in 84.277 CPU sec, 2.674 real sec
sorting block 16, #reads = 1079267 mem = 536881582 peak = 1031655424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 282379, 5, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 235, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (291.55, 164.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 333, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (349.01, 170.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 240, 806)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1994)
[M::mem_pestat] mean and std.dev: (411.19, 321.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2588)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682106 sequences (100000000 bp) in 1.929 CPU sec, 2.063 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282234, 3, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (354, 421, 914)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2034)
[M::mem_pestat] mean and std.dev: (539.67, 303.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2594)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 333, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (348.52, 169.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (290, 358, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (350.35, 163.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682294 sequences (100000167 bp) in 1.310 CPU sec, 1.301 real sec...
[M::mem_process_seqs] Processed 682250 reads in 134.525 CPU sec, 4.294 real sec
sorting block 17, #reads = 1078728 mem = 536877922 peak = 1031655424
[M::mem_process_seqs] Processed 681824 reads in 85.140 CPU sec, 2.715 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282585, 2, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (248, 356, 2706)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7622)
[M::mem_pestat] mean and std.dev: (894.94, 1040.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10080)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 328, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (344.83, 168.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 355, 639)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1433)
[M::mem_pestat] mean and std.dev: (400.53, 224.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1830)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 18, #reads = 1079404 mem = 536880755 peak = 1040687104
[M::process] read 681972 sequences (100000150 bp) in 2.197 CPU sec, 2.324 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 282321, 3, 18)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 325, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (342.06, 166.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 247, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (302.83, 161.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RR
[M::process] read 681620 sequences (100000103 bp) in 1.310 CPU sec, 1.312 real sec...
[M::mem_process_seqs] Processed 682106 reads in 134.197 CPU sec, 4.267 real sec
[M::mem_process_seqs] Processed 682294 reads in 84.999 CPU sec, 2.698 real sec
sorting block 19, #reads = 1079746 mem = 536879117 peak = 1040687104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282670, 1, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 375, 1068)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2800)
[M::mem_pestat] mean and std.dev: (807.23, 906.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4433)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 327, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (343.67, 166.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 392, 540)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1202)
[M::mem_pestat] mean and std.dev: (379.31, 205.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1533)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681350 sequences (100000169 bp) in 1.977 CPU sec, 2.118 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282029, 4, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 225, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (202.12, 72.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 330, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (346.08, 168.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 300, 1288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3428)
[M::mem_pestat] mean and std.dev: (651.57, 552.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4498)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681008 sequences (100000068 bp) in 1.290 CPU sec, 1.288 real sec...
[M::mem_process_seqs] Processed 681972 reads in 134.423 CPU sec, 4.282 real sec
[M::mem_process_seqs] Processed 681620 reads in 84.897 CPU sec, 2.689 real sec
sorting block 20, #reads = 1079441 mem = 536877990 peak = 1040687104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282274, 6, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (226, 283, 716)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1696)
[M::mem_pestat] mean and std.dev: (362.57, 249.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2186)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 330, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (347.15, 168.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 380, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (357.19, 117.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683470 sequences (100000046 bp) in 2.316 CPU sec, 2.464 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282052, 4, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 446, 690)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1706)
[M::mem_pestat] mean and std.dev: (537.09, 447.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2327)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 333, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (349.75, 169.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (398, 474, 1195)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2789)
[M::mem_pestat] mean and std.dev: (703.96, 446.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3586)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682434 sequences (100000159 bp) in 1.260 CPU sec, 1.261 real sec...
[M::mem_process_seqs] Processed 681350 reads in 133.059 CPU sec, 4.228 real sec
[M::mem_process_seqs] Processed 681008 reads in 84.276 CPU sec, 2.671 real sec
sorting block 21, #reads = 1079565 mem = 536878828 peak = 1040687104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283575, 5, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 330, 547)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1261)
[M::mem_pestat] mean and std.dev: (386.29, 220.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1618)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 322, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (339.64, 167.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 344, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (335.35, 116.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681598 sequences (100000070 bp) in 2.031 CPU sec, 2.172 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 283064, 13, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (216, 240, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (275.29, 113.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 332, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (348.35, 170.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (278, 284, 756)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1712)
[M::mem_pestat] mean and std.dev: (389.38, 360.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2190)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 171, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (206.23, 110.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 680732 sequences (100000275 bp) in 1.290 CPU sec, 1.291 real sec...
[M::mem_process_seqs] Processed 683470 reads in 132.777 CPU sec, 4.229 real sec
sorting block 22, #reads = 1079309 mem = 536877973 peak = 1040687104
[M::mem_process_seqs] Processed 682434 reads in 83.615 CPU sec, 2.647 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282262, 10, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (381, 516, 703)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1347)
[M::mem_pestat] mean and std.dev: (541.98, 280.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1669)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 339, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (353.32, 171.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 408, 704)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1798)
[M::mem_pestat] mean and std.dev: (332.12, 202.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2345)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 476, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1159)
[M::mem_pestat] mean and std.dev: (487.36, 356.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1912)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 23, #reads = 1081767 mem = 536875983 peak = 1064681472
[M::process] read 681666 sequences (100000162 bp) in 2.298 CPU sec, 2.432 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 282316, 7, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 211, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (198.25, 65.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 346, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (359.34, 173.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 681664 sequences (100000287 bp) in 1.310 CPU sec, 1.310 real sec...
[M::mem_process_seqs] Processed 681598 reads in 134.130 CPU sec, 4.261 real sec
[M::mem_process_seqs] Processed 680732 reads in 84.348 CPU sec, 2.670 real sec
sorting block 24, #reads = 1080901 mem = 536878606 peak = 1064681472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 282170, 2, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (218, 361, 686)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1622)
[M::mem_pestat] mean and std.dev: (517.56, 411.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2162)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 340, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (354.61, 173.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 291, 588)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1414)
[M::mem_pestat] mean and std.dev: (422.73, 309.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1827)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682278 sequences (100000087 bp) in 1.947 CPU sec, 2.063 real sec...
[M::process] read 682124 sequences (100000098 bp) in 1.290 CPU sec, 1.290 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282164, 3, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 204, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (226.80, 105.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 336, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (350.94, 170.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 242, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (281.56, 152.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681666 reads in 135.481 CPU sec, 4.315 real sec
[M::mem_process_seqs] Processed 681664 reads in 85.148 CPU sec, 2.699 real sec
sorting block 25, #reads = 1079983 mem = 536879111 peak = 1064681472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282740, 4, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 244, 548)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1236)
[M::mem_pestat] mean and std.dev: (409.63, 290.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1580)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 328, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (344.35, 167.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (317, 367, 614)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1208)
[M::mem_pestat] mean and std.dev: (426.06, 215.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1505)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681858 sequences (100000002 bp) in 2.321 CPU sec, 2.441 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 282226, 7, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 395, 682)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1632)
[M::mem_pestat] mean and std.dev: (368.71, 220.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2107)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 327, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (342.61, 165.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 412, 549)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1157)
[M::mem_pestat] mean and std.dev: (420.83, 179.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1461)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681924 sequences (100000211 bp) in 1.290 CPU sec, 1.292 real sec...
[M::mem_process_seqs] Processed 682278 reads in 137.156 CPU sec, 4.359 real sec
[M::mem_process_seqs] Processed 682124 reads in 86.344 CPU sec, 2.740 real sec
sorting block 26, #reads = 1080016 mem = 536881440 peak = 1064681472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 282219, 8, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 373, 610)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1478)
[M::mem_pestat] mean and std.dev: (346.05, 191.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1912)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 328, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (344.31, 166.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 267, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (279.69, 120.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681312 sequences (100000283 bp) in 1.983 CPU sec, 2.097 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 282993, 2, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (240, 308, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (324.07, 141.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 680706 sequences (100000068 bp) in 1.290 CPU sec, 1.290 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (207, 329, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (345.81, 168.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 276, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (270.80, 122.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 981)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681858 reads in 136.573 CPU sec, 4.351 real sec
sorting block 27, #reads = 1079545 mem = 536878066 peak = 1064681472
[M::mem_process_seqs] Processed 681924 reads in 85.821 CPU sec, 2.722 real sec
sorting block 28, #reads = 1079411 mem = 536877432 peak = 1070120960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282698, 2, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 357, 830)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2090)
[M::mem_pestat] mean and std.dev: (354.36, 226.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2720)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 331, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (347.03, 166.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 273, 760)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1868)
[M::mem_pestat] mean and std.dev: (405.35, 316.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2422)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686526 sequences (100000107 bp) in 2.328 CPU sec, 2.450 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282347, 1, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 227, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611)
[M::mem_pestat] mean and std.dev: (224.43, 87.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 761)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 336, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (350.76, 168.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 687460 sequences (100000172 bp) in 1.360 CPU sec, 1.360 real sec...
[M::mem_process_seqs] Processed 681312 reads in 134.952 CPU sec, 4.285 real sec
[M::mem_process_seqs] Processed 680706 reads in 84.989 CPU sec, 2.692 real sec
sorting block 29, #reads = 1078753 mem = 536879486 peak = 1070120960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 284205, 4, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 275, 765)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1965)
[M::mem_pestat] mean and std.dev: (449.38, 347.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2565)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 306, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (328.24, 165.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 218, 556)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1406)
[M::mem_pestat] mean and std.dev: (340.89, 288.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1831)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685574 sequences (100000247 bp) in 1.931 CPU sec, 2.018 real sec...
[M::process] read 684872 sequences (100000017 bp) in 1.350 CPU sec, 1.342 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 285204, 2, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 226, 623)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1473)
[M::mem_pestat] mean and std.dev: (342.40, 191.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1898)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 303, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (324.93, 164.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 183, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (77, 342)
[M::mem_pestat] mean and std.dev: (203.80, 45.03)
[M::mem_pestat] low and high boundaries for proper pairs: (24, 395)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686526 reads in 141.476 CPU sec, 4.506 real sec
[M::mem_process_seqs] Processed 687460 reads in 89.110 CPU sec, 2.828 real sec
sorting block 30, #reads = 1078961 mem = 536879822 peak = 1070120960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 283947, 4, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (213, 314, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684)
[M::mem_pestat] mean and std.dev: (291.80, 81.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 315, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (331.85, 163.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 392, 901)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2199)
[M::mem_pestat] mean and std.dev: (572.91, 414.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2848)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687006 sequences (100000155 bp) in 2.126 CPU sec, 2.238 real sec...
[M::process] read 686466 sequences (100000153 bp) in 1.370 CPU sec, 1.373 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283912, 1, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 242, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (215.93, 67.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 320, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (335.11, 164.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (303, 414, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (382.46, 159.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685574 reads in 142.965 CPU sec, 4.552 real sec
sorting block 31, #reads = 1080367 mem = 536881350 peak = 1070120960
[M::mem_process_seqs] Processed 684872 reads in 89.604 CPU sec, 2.844 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 284520, 5, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 239, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (270.11, 166.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 303, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (323.44, 161.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (285, 410, 905)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2145)
[M::mem_pestat] mean and std.dev: (554.22, 327.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2765)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 32, #reads = 1078029 mem = 536877565 peak = 1090269184
[M::process] read 684922 sequences (100000071 bp) in 2.075 CPU sec, 2.191 real sec...
[M::process] read 686696 sequences (100000142 bp) in 1.430 CPU sec, 1.426 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 284484, 7, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (287, 287, 540)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046)
[M::mem_pestat] mean and std.dev: (333.67, 130.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 306, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (324.91, 160.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 687006 reads in 149.523 CPU sec, 4.760 real sec
[M::mem_process_seqs] Processed 686466 reads in 95.451 CPU sec, 3.031 real sec
sorting block 33, #reads = 1077508 mem = 536887886 peak = 1090269184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 283804, 4, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 219, 1602)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4466)
[M::mem_pestat] mean and std.dev: (676.69, 686.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5898)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 314, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (331.83, 162.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (295, 408, 1323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3379)
[M::mem_pestat] mean and std.dev: (877.17, 816.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4407)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684222 sequences (100000045 bp) in 2.378 CPU sec, 2.488 real sec...
[M::process] read 684000 sequences (100000105 bp) in 1.380 CPU sec, 1.383 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 284517, 4, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 281, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1084)
[M::mem_pestat] mean and std.dev: (306.83, 183.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1383)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 302, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (320.22, 156.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 242, 588)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1488)
[M::mem_pestat] mean and std.dev: (349.57, 294.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1938)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684922 reads in 153.357 CPU sec, 4.875 real sec
[M::mem_process_seqs] Processed 686696 reads in 100.746 CPU sec, 3.199 real sec
sorting block 34, #reads = 1075950 mem = 536878471 peak = 1090269184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283427, 5, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (214, 281, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1138)
[M::mem_pestat] mean and std.dev: (320.04, 168.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1446)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 316, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (333.01, 161.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 268, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075)
[M::mem_pestat] mean and std.dev: (307.44, 188.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1386)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 689374 sequences (100000225 bp) in 2.145 CPU sec, 2.267 real sec...
[M::process] read 688694 sequences (100000097 bp) in 1.380 CPU sec, 1.379 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 283765, 1, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (395, 513, 917)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1961)
[M::mem_pestat] mean and std.dev: (584.33, 316.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2483)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 317, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (334.42, 163.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 401, 596)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1430)
[M::mem_pestat] mean and std.dev: (392.15, 285.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1847)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684222 reads in 154.762 CPU sec, 4.928 real sec
[M::mem_process_seqs] Processed 684000 reads in 97.881 CPU sec, 3.107 real sec
sorting block 35, #reads = 1072839 mem = 536879275 peak = 1090269184
[M::process] read 686714 sequences (100000133 bp) in 2.256 CPU sec, 2.327 real sec...
[M::process] read 685868 sequences (100000274 bp) in 1.360 CPU sec, 1.361 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 285102, 2, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 222, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605)
[M::mem_pestat] mean and std.dev: (255.28, 134.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 293, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (314.77, 157.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (317, 361, 768)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1670)
[M::mem_pestat] mean and std.dev: (531.09, 435.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2273)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688694 reads in 82.086 CPU sec, 2.597 real sec
sorting block 36, #reads = 1073186 mem = 536883832 peak = 1103568896
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285709, 9, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 287, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (313.03, 159.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 176, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1070)
[M::mem_pestat] mean and std.dev: (274.92, 157.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1368)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 284650, 3, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 262, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (257.78, 121.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 305, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (322.94, 159.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 411, 609)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1467)
[M::mem_pestat] mean and std.dev: (319.92, 161.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1896)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686342 sequences (100000124 bp) in 1.681 CPU sec, 1.683 real sec...
[M::mem_process_seqs] Processed 689374 reads in 237.232 CPU sec, 7.538 real sec
[M::mem_process_seqs] Processed 686714 reads in 110.727 CPU sec, 3.535 real sec
sorting block 37, #reads = 1074222 mem = 536887162 peak = 1103568896
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 284907, 3, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (199, 306, 611)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1435)
[M::mem_pestat] mean and std.dev: (359.12, 252.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1847)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 310, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (327.60, 161.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 348, 562)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1204)
[M::mem_pestat] mean and std.dev: (403.94, 232.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1525)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686006 sequences (100000115 bp) in 2.111 CPU sec, 2.244 real sec...
[M::process] read 686570 sequences (100000192 bp) in 1.330 CPU sec, 1.334 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284262, 4, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 250, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (267.00, 150.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 307, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (325.91, 161.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 308, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (295.85, 119.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685868 reads in 144.340 CPU sec, 4.600 real sec
sorting block 38, #reads = 1074408 mem = 536877525 peak = 1103568896
[M::mem_process_seqs] Processed 686342 reads in 90.142 CPU sec, 2.868 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 284809, 10, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 241, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557)
[M::mem_pestat] mean and std.dev: (220.18, 67.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 685)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 308, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (326.40, 161.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (83, 1415, 2668)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7838)
[M::mem_pestat] mean and std.dev: (1193.33, 1004.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10423)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 257, 889)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2249)
[M::mem_pestat] mean and std.dev: (471.57, 432.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2929)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 39, #reads = 1077847 mem = 536881455 peak = 1140490240
[M::process] read 683768 sequences (100000071 bp) in 2.330 CPU sec, 2.485 real sec...
[M::process] read 682694 sequences (100000098 bp) in 1.360 CPU sec, 1.363 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 284628, 5, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 216, 539)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1283)
[M::mem_pestat] mean and std.dev: (326.75, 240.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1655)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 305, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (324.39, 160.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 367, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684)
[M::mem_pestat] mean and std.dev: (293.00, 98.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686006 reads in 152.904 CPU sec, 4.861 real sec
[M::mem_process_seqs] Processed 686570 reads in 99.000 CPU sec, 3.144 real sec
sorting block 40, #reads = 1075170 mem = 536878993 peak = 1140490240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283771, 5, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 234, 645)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1571)
[M::mem_pestat] mean and std.dev: (261.88, 166.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2034)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 323, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (341.44, 169.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 252, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (274.64, 124.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682440 sequences (100000278 bp) in 2.033 CPU sec, 2.170 real sec...
[M::process] read 686308 sequences (100000268 bp) in 1.380 CPU sec, 1.385 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282819, 6, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 236, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1183)
[M::mem_pestat] mean and std.dev: (307.13, 195.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1525)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 330, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (348.53, 172.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 246, 1639)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4563)
[M::mem_pestat] mean and std.dev: (689.81, 948.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6025)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683768 reads in 146.036 CPU sec, 4.646 real sec
[M::mem_process_seqs] Processed 682694 reads in 91.821 CPU sec, 2.917 real sec
sorting block 41, #reads = 1074292 mem = 536879997 peak = 1140490240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 283150, 11, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (123, 169, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1086)
[M::mem_pestat] mean and std.dev: (286.72, 190.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1407)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 332, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (351.66, 175.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (217, 562, 755)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1831)
[M::mem_pestat] mean and std.dev: (462.64, 268.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2369)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 220, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (261.08, 137.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 685596 sequences (100000169 bp) in 2.165 CPU sec, 2.287 real sec...
[M::process] read 683828 sequences (100000111 bp) in 1.310 CPU sec, 1.314 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284243, 5, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 189, 213)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 309)
[M::mem_pestat] mean and std.dev: (174.33, 23.71)
[M::mem_pestat] low and high boundaries for proper pairs: (21, 357)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 309, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (332.86, 170.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 294, 542)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1156)
[M::mem_pestat] mean and std.dev: (322.97, 199.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1463)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682440 reads in 147.596 CPU sec, 4.694 real sec
[M::mem_process_seqs] Processed 686308 reads in 94.204 CPU sec, 2.998 real sec
sorting block 42, #reads = 1076112 mem = 536881704 peak = 1140490240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 284185, 1, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 223, 531)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1321)
[M::mem_pestat] mean and std.dev: (304.15, 209.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1716)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 316, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (337.39, 171.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 355, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1063)
[M::mem_pestat] mean and std.dev: (347.28, 169.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1358)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683038 sequences (100000257 bp) in 1.915 CPU sec, 2.026 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 283306, 1, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 223, 1097)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2981)
[M::mem_pestat] mean and std.dev: (496.81, 430.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3923)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 325, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (342.50, 170.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (137, 178, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (200.78, 88.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685294 sequences (100000129 bp) in 1.320 CPU sec, 1.328 real sec...
[M::mem_process_seqs] Processed 685596 reads in 136.937 CPU sec, 4.365 real sec
sorting block 43, #reads = 1074949 mem = 536878790 peak = 1140490240
[M::mem_process_seqs] Processed 683828 reads in 85.528 CPU sec, 2.712 real sec
sorting block 44, #reads = 1080123 mem = 536878404 peak = 1151627264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 283838, 5, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (293, 307, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (149, 509)
[M::mem_pestat] mean and std.dev: (338.62, 58.18)
[M::mem_pestat] low and high boundaries for proper pairs: (77, 581)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 332, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (348.63, 172.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 306, 637)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1559)
[M::mem_pestat] mean and std.dev: (404.81, 325.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2020)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684400 sequences (100000072 bp) in 2.323 CPU sec, 2.451 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 284505, 7, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 279, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1059)
[M::mem_pestat] mean and std.dev: (293.20, 163.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1367)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 315, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (334.90, 168.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 285, 535)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1219)
[M::mem_pestat] mean and std.dev: (461.57, 368.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1934)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683002 sequences (100000178 bp) in 1.330 CPU sec, 1.334 real sec...
[M::mem_process_seqs] Processed 683038 reads in 136.651 CPU sec, 4.341 real sec
[M::mem_process_seqs] Processed 685294 reads in 86.758 CPU sec, 2.750 real sec
sorting block 45, #reads = 1079730 mem = 536880670 peak = 1151627264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283744, 0, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (223, 281, 816)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2002)
[M::mem_pestat] mean and std.dev: (406.71, 316.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2595)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 322, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (341.05, 171.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 264, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (274.63, 113.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683624 sequences (100000142 bp) in 1.959 CPU sec, 2.074 real sec...
[M::process] read 683406 sequences (100000299 bp) in 1.340 CPU sec, 1.336 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 283165, 6, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 218, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (228.92, 94.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 329, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (347.47, 173.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 251, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (49, 499)
[M::mem_pestat] mean and std.dev: (245.46, 63.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 589)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684400 reads in 140.261 CPU sec, 4.468 real sec
[M::mem_process_seqs] Processed 683002 reads in 87.530 CPU sec, 2.775 real sec
sorting block 46, #reads = 1080284 mem = 536877670 peak = 1151627264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 283383, 3, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 387, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (351.29, 183.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 323, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (343.29, 172.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 313, 603)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1343)
[M::mem_pestat] mean and std.dev: (369.71, 222.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1713)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683332 sequences (100000299 bp) in 2.279 CPU sec, 2.400 real sec...
[M::process] read 683734 sequences (100000151 bp) in 1.370 CPU sec, 1.368 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 283405, 1, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 352, 814)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2052)
[M::mem_pestat] mean and std.dev: (480.70, 382.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2671)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 324, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (345.19, 173.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 260, 575)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1287)
[M::mem_pestat] mean and std.dev: (365.60, 218.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1643)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683624 reads in 145.462 CPU sec, 4.626 real sec
sorting block 47, #reads = 1078420 mem = 536882489 peak = 1151627264
[M::mem_process_seqs] Processed 683406 reads in 91.734 CPU sec, 2.911 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283314, 6, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (241, 350, 569)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1225)
[M::mem_pestat] mean and std.dev: (349.38, 164.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1553)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 324, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (346.61, 176.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 213, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596)
[M::mem_pestat] mean and std.dev: (228.50, 85.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 48, #reads = 1076320 mem = 536880657 peak = 1151627264
[M::process] read 688844 sequences (100000269 bp) in 2.149 CPU sec, 2.269 real sec...
[M::process] read 686084 sequences (100000014 bp) in 1.370 CPU sec, 1.372 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 283250, 5, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 280, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (247.10, 97.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 319, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (342.95, 174.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 293, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (303.43, 130.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683332 reads in 147.576 CPU sec, 4.697 real sec
[M::mem_process_seqs] Processed 683734 reads in 95.296 CPU sec, 3.022 real sec
sorting block 49, #reads = 1075798 mem = 536878084 peak = 1151627264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 285216, 5, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (300, 372, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (42, 687)
[M::mem_pestat] mean and std.dev: (303.00, 98.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 293, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (320.46, 166.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 300, 936)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2380)
[M::mem_pestat] mean and std.dev: (521.61, 489.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3102)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684442 sequences (100000105 bp) in 2.183 CPU sec, 2.271 real sec...
[M::process] read 683826 sequences (100000185 bp) in 1.340 CPU sec, 1.346 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 284777, 4, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 215, 225)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 295)
[M::mem_pestat] mean and std.dev: (195.36, 29.96)
[M::mem_pestat] low and high boundaries for proper pairs: (76, 330)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 313, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (333.34, 168.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 273, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1071)
[M::mem_pestat] mean and std.dev: (304.46, 157.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1368)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688844 reads in 148.731 CPU sec, 4.727 real sec
[M::mem_process_seqs] Processed 686084 reads in 91.934 CPU sec, 2.928 real sec
sorting block 50, #reads = 1073456 mem = 536876569 peak = 1151627264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 283920, 5, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 280, 557)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1365)
[M::mem_pestat] mean and std.dev: (258.29, 150.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1769)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 323, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (340.00, 168.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 565, 647)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1477)
[M::mem_pestat] mean and std.dev: (541.55, 315.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1892)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683674 sequences (100000260 bp) in 1.902 CPU sec, 1.988 real sec...
[M::process] read 682868 sequences (100000222 bp) in 1.330 CPU sec, 1.336 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283217, 5, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (245, 351, 916)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2258)
[M::mem_pestat] mean and std.dev: (584.21, 483.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2929)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 327, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (343.58, 169.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 216, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507)
[M::mem_pestat] mean and std.dev: (220.17, 82.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 617)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684442 reads in 143.010 CPU sec, 4.557 real sec
[M::mem_process_seqs] Processed 683826 reads in 89.959 CPU sec, 2.856 real sec
sorting block 51, #reads = 1076746 mem = 536878966 peak = 1151627264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 283729, 0, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (237, 306, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (296.50, 128.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1105)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 322, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (340.00, 167.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (313, 524, 865)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1969)
[M::mem_pestat] mean and std.dev: (496.18, 263.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2521)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682786 sequences (100000241 bp) in 2.216 CPU sec, 2.321 real sec...
[M::process] read 682334 sequences (100000198 bp) in 1.310 CPU sec, 1.316 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282934, 4, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 187, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555)
[M::mem_pestat] mean and std.dev: (218.65, 62.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 682)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 324, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (340.03, 164.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 325, 828)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2042)
[M::mem_pestat] mean and std.dev: (546.40, 444.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2649)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683674 reads in 144.684 CPU sec, 4.603 real sec
sorting block 52, #reads = 1075844 mem = 536879896 peak = 1151627264
[M::mem_process_seqs] Processed 682868 reads in 91.609 CPU sec, 2.910 real sec
sorting block 53, #reads = 1077385 mem = 536885685 peak = 1178419200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282766, 7, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548)
[M::mem_pestat] mean and std.dev: (193.76, 63.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 675)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 322, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (338.41, 163.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 317, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623)
[M::mem_pestat] mean and std.dev: (274.67, 110.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 768)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682368 sequences (100000244 bp) in 1.896 CPU sec, 1.979 real sec...
[M::process] read 681984 sequences (100000252 bp) in 1.320 CPU sec, 1.324 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282909, 8, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 351, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (309.00, 128.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 321, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (337.89, 163.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 264, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042)
[M::mem_pestat] mean and std.dev: (323.58, 186.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1323)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682786 reads in 143.542 CPU sec, 4.575 real sec
[M::process] read 681848 sequences (100000258 bp) in 1.571 CPU sec, 1.571 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282483, 10, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (205, 373, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862)
[M::mem_pestat] mean and std.dev: (336.76, 173.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1081)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 320, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (338.53, 165.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (43, 1714, 2444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7246)
[M::mem_pestat] mean and std.dev: (1550.80, 1393.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9647)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 298, 542)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1206)
[M::mem_pestat] mean and std.dev: (363.55, 198.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1538)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682368 reads in 81.045 CPU sec, 2.617 real sec
sorting block 54, #reads = 1075511 mem = 536879294 peak = 1178419200
[M::mem_process_seqs] Processed 682334 reads in 193.114 CPU sec, 6.184 real sec
[M::process] read 31796 sequences (4651260 bp) in 0.140 CPU sec, 0.144 real sec...
sorting block 55, #reads = 1078066 mem = 536879838 peak = 1188937728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282098, 4, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 263, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (274.00, 136.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 323, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (340.63, 165.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 248, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (283.42, 130.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282574, 8, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 291, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (311.58, 121.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 327, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (344.97, 169.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 255, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (264.16, 81.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 13199, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 315, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (335.43, 166.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681984 reads in 152.354 CPU sec, 4.931 real sec
[M::mem_process_seqs] Processed 681848 reads in 89.237 CPU sec, 2.883 real sec
[M::mem_process_seqs] Processed 31796 reads in 89.819 CPU sec, 2.906 real sec
sorting block 56, #reads = 1078230 mem = 536879718 peak = 1188937728
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 61536848
overall: 69868367872 mem 8330.362 user 351.854 sys 770.039 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_3U6-T1-TDNA-1_A23MHWWLT4_1.L001\tPU:L001\tSM:1136_3U6-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_3U6-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0002.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0002.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 770.074 sec; CPU: 8682.220 sec
sorting block 57, #reads = 404883 mem = 201956708 peak = 1188937728
uniq header count 1
merging 58 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3595782592
output reads: 61865000
bam_mem_sort: 58 calls 21.810 user 0.272 sys 22.238 real
bam_write: 58 calls 48.968 user 0.998 sys 50.276 real
execute: 1 calls 11.638 user 0.604 sys 790.173 real
merge_files: 1 calls 11.611 user 0.593 sys 19.585 real
parse_chunk: 919 calls 28.570 user 8.075 sys 37.318 real
read_chunk: 2479 calls 23.885 user 21.129 sys 231.700 real
sort_block: 1 calls 18.324 user 20.519 sys 223.658 real
write_chunk: 1558 calls 11.304 user 0.434 sys 18.893 real
overall: 2715475968 mem 738.873 user 37.677 sys 790.195 real
ls: cannot access '*.cram': No such file or directory