Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f0/fd411bfa2c85ed253ff7dbce25bc8c/1029_N5D-T1-TDNA-1_A23MHWWLT4_1/0002.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ba/aa3f8990ce9e7f4dad28e4beda8b03/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f0/fd411bfa2c85ed253ff7dbce25bc8c/1029_N5D-T1-TDNA-1_A23MHWWLT4_1/0002.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ba/aa3f8990ce9e7f4dad28e4beda8b03/.command.run
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1029_N5D-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
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No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1675.742 CPU sec, 1322.955 real sec
[M::process] read 680980 sequences (100000055 bp) in 0.978 CPU sec, 0.984 real sec...
[M::process] read 681400 sequences (100000049 bp) in 1.183 CPU sec, 1.196 real sec...
[M::process] read 682460 sequences (100000082 bp) in 1.175 CPU sec, 1.192 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 277907, 11, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 220, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (235.00, 82.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 705)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 354, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (367.78, 174.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 268, 1726)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5122)
[M::mem_pestat] mean and std.dev: (739.27, 822.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6820)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 222, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864)
[M::mem_pestat] mean and std.dev: (239.92, 113.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1103)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 679944 sequences (100000046 bp) in 1.131 CPU sec, 1.145 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 278301, 4, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 294, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (256.56, 109.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 343, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (358.61, 172.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 300, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (324.77, 141.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1303)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 278769, 0, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 253, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (241.17, 101.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 956)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 352, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (366.49, 175.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 311, 761)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1817)
[M::mem_pestat] mean and std.dev: (368.04, 260.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2345)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680980 reads in 235.749 CPU sec, 7.592 real sec
[M::mem_process_seqs] Processed 682460 reads in 164.039 CPU sec, 5.336 real sec
[M::mem_process_seqs] Processed 681400 reads in 179.318 CPU sec, 5.839 real sec
sorting block 0, #reads = 1069110 mem = 536880586 peak = 604602368
[M::process] read 681766 sequences (100000055 bp) in 1.600 CPU sec, 1.729 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 277926, 5, 57)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 230, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (228.40, 85.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 362, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (375.21, 177.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 295, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (306.34, 152.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681450 sequences (100000171 bp) in 1.219 CPU sec, 1.231 real sec...
[M::process] read 680630 sequences (100000073 bp) in 1.130 CPU sec, 1.124 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 277705, 6, 51)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 319, 573)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1365)
[M::mem_pestat] mean and std.dev: (400.32, 310.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1761)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 347, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (363.02, 174.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 349, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (351.00, 165.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 679944 reads in 152.472 CPU sec, 4.866 real sec
sorting block 1, #reads = 1070066 mem = 536879412 peak = 916029440
[M::mem_process_seqs] Processed 681766 reads in 98.074 CPU sec, 3.132 real sec
sorting block 2, #reads = 1070690 mem = 536878554 peak = 974598144
[M::process] read 680426 sequences (100000025 bp) in 1.526 CPU sec, 1.618 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 277533, 3, 51)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 299, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (319.77, 169.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1291)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 347, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (362.95, 173.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 265, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (288.54, 158.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681222 sequences (100000277 bp) in 1.230 CPU sec, 1.230 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 277295, 2, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 284, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (247.80, 94.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 692)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 349, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (364.12, 172.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 350, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (308.07, 116.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 277135, 1, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 204, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (235.97, 134.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 349, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (364.30, 172.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 299, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (295.60, 87.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681450 reads in 221.087 CPU sec, 7.098 real sec
[M::mem_process_seqs] Processed 680630 reads in 165.502 CPU sec, 5.343 real sec
[M::mem_process_seqs] Processed 680426 reads in 180.456 CPU sec, 5.839 real sec
sorting block 3, #reads = 1069846 mem = 536877473 peak = 974598144
[M::process] read 679848 sequences (100000128 bp) in 1.818 CPU sec, 1.991 real sec...
sorting block 4, #reads = 1069542 mem = 536879265 peak = 974598144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 277672, 12, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 270, 859)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2177)
[M::mem_pestat] mean and std.dev: (430.12, 407.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2836)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 342, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (359.30, 171.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (773, 812, 3965)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10349)
[M::mem_pestat] mean and std.dev: (2203.08, 2596.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 13541)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 325, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1155)
[M::mem_pestat] mean and std.dev: (347.88, 213.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1476)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 680908 sequences (100000300 bp) in 1.420 CPU sec, 1.491 real sec...
[M::process] read 680700 sequences (100000002 bp) in 1.210 CPU sec, 1.211 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 277140, 5, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 318, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1197)
[M::mem_pestat] mean and std.dev: (319.65, 186.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1533)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 352, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (365.51, 170.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 256, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (279.64, 127.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681222 reads in 157.057 CPU sec, 5.013 real sec
[M::mem_process_seqs] Processed 679848 reads in 99.239 CPU sec, 3.155 real sec
sorting block 5, #reads = 1069841 mem = 536879482 peak = 974598144
[M::process] read 680200 sequences (100000115 bp) in 1.687 CPU sec, 1.828 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 277511, 4, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (221, 248, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (45, 485)
[M::mem_pestat] mean and std.dev: (251.84, 75.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 573)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 345, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (360.68, 171.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 220, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (271.41, 157.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681170 sequences (100000008 bp) in 1.220 CPU sec, 1.223 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 277591, 8, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 244, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617)
[M::mem_pestat] mean and std.dev: (228.56, 77.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 347, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (363.83, 174.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 323, 540)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1100)
[M::mem_pestat] mean and std.dev: (365.31, 198.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1380)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 277534, 3, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 313, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771)
[M::mem_pestat] mean and std.dev: (292.12, 100.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 355, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (370.75, 177.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 338, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (291.94, 119.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680908 reads in 216.550 CPU sec, 6.948 real sec
[M::mem_process_seqs] Processed 680700 reads in 160.644 CPU sec, 5.170 real sec
sorting block 6, #reads = 1069915 mem = 536880572 peak = 974598144
[M::mem_process_seqs] Processed 680200 reads in 175.965 CPU sec, 5.685 real sec
[M::process] read 681546 sequences (100000267 bp) in 1.787 CPU sec, 1.973 real sec...
sorting block 7, #reads = 1070079 mem = 536877447 peak = 974598144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 278370, 5, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 259, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (238.79, 88.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 349, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (364.55, 174.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 339, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (328.19, 143.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1087)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681702 sequences (100000211 bp) in 1.500 CPU sec, 1.576 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 277484, 13, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 214, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702)
[M::mem_pestat] mean and std.dev: (254.21, 142.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 881)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 353, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (367.21, 176.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (313, 459, 1269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3181)
[M::mem_pestat] mean and std.dev: (617.69, 391.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4137)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 356, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046)
[M::mem_pestat] mean and std.dev: (301.29, 148.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1329)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 680798 sequences (100000224 bp) in 1.240 CPU sec, 1.242 real sec...
[M::mem_process_seqs] Processed 681170 reads in 149.893 CPU sec, 4.790 real sec
[M::mem_process_seqs] Processed 681546 reads in 96.105 CPU sec, 3.052 real sec
sorting block 8, #reads = 1071005 mem = 536879386 peak = 974598144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 278457, 3, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (209, 341, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (321.22, 133.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 350, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (364.91, 175.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 326, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (295.03, 100.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681554 sequences (100000073 bp) in 1.761 CPU sec, 1.929 real sec...
[M::process] read 680938 sequences (100000175 bp) in 1.270 CPU sec, 1.270 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 277642, 14, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (307, 478, 630)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1276)
[M::mem_pestat] mean and std.dev: (442.90, 214.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1599)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 357, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (370.90, 176.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 232, 2136)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6400)
[M::mem_pestat] mean and std.dev: (1210.43, 1703.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8532)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 329, 610)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1332)
[M::mem_pestat] mean and std.dev: (402.75, 220.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1693)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681702 reads in 146.803 CPU sec, 4.700 real sec
[M::mem_process_seqs] Processed 680798 reads in 93.634 CPU sec, 2.995 real sec
sorting block 9, #reads = 1072504 mem = 536879019 peak = 974598144
[M::process] read 679830 sequences (100000145 bp) in 1.810 CPU sec, 1.912 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 279038, 9, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (219, 271, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (257.81, 108.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 747)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 350, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (365.49, 175.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 250, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575)
[M::mem_pestat] mean and std.dev: (227.94, 75.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 702)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681106 sequences (100000268 bp) in 1.290 CPU sec, 1.297 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 278331, 2, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (243, 277, 534)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1116)
[M::mem_pestat] mean and std.dev: (318.78, 171.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1407)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 352, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (367.27, 175.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 280, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (287.38, 99.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681554 reads in 146.828 CPU sec, 4.696 real sec
sorting block 10, #reads = 1071654 mem = 536880338 peak = 974598144
[M::mem_process_seqs] Processed 680938 reads in 94.165 CPU sec, 3.013 real sec
sorting block 11, #reads = 1073007 mem = 536877464 peak = 998043648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 277614, 4, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (224, 316, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1148)
[M::mem_pestat] mean and std.dev: (367.62, 279.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1484)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 357, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (372.14, 176.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 372, 619)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1443)
[M::mem_pestat] mean and std.dev: (325.54, 171.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1855)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681088 sequences (100000246 bp) in 2.074 CPU sec, 2.235 real sec...
[M::process] read 680074 sequences (100000079 bp) in 1.300 CPU sec, 1.305 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 277704, 3, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 240, 740)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1898)
[M::mem_pestat] mean and std.dev: (276.94, 237.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2477)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 347, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (363.94, 175.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 259, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (336.89, 197.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1304)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 679830 reads in 151.087 CPU sec, 4.828 real sec
[M::mem_process_seqs] Processed 681106 reads in 97.001 CPU sec, 3.097 real sec
sorting block 12, #reads = 1070218 mem = 536877879 peak = 998043648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 278087, 1, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 262, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577)
[M::mem_pestat] mean and std.dev: (235.34, 93.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 708)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 345, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (362.26, 174.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 296, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (294.35, 138.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681238 sequences (100000149 bp) in 2.191 CPU sec, 2.370 real sec...
[M::process] read 681388 sequences (100000285 bp) in 1.350 CPU sec, 1.354 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 277729, 6, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (124, 185, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (208.86, 127.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 352, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (368.05, 175.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 299, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1086)
[M::mem_pestat] mean and std.dev: (292.58, 129.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1376)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681088 reads in 150.900 CPU sec, 4.820 real sec
[M::mem_process_seqs] Processed 680074 reads in 96.652 CPU sec, 3.081 real sec
sorting block 13, #reads = 1070480 mem = 536877491 peak = 998043648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 278244, 2, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 238, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (225.57, 109.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 344, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (361.09, 173.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 275, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (274.54, 137.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 914)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681924 sequences (100000157 bp) in 2.104 CPU sec, 2.224 real sec...
[M::process] read 682700 sequences (100000265 bp) in 1.350 CPU sec, 1.358 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 278628, 9, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 279, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (252.23, 113.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 344, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (362.59, 175.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (279, 396, 692)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1518)
[M::mem_pestat] mean and std.dev: (376.79, 238.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1931)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681238 reads in 150.637 CPU sec, 4.808 real sec
sorting block 14, #reads = 1070217 mem = 536879634 peak = 998043648
[M::mem_process_seqs] Processed 681388 reads in 96.103 CPU sec, 3.068 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278601, 7, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (221, 293, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737)
[M::mem_pestat] mean and std.dev: (292.94, 106.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 909)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 343, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (360.35, 174.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 264, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638)
[M::mem_pestat] mean and std.dev: (280.41, 111.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 782)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681656 sequences (100000040 bp) in 2.256 CPU sec, 2.401 real sec...
[M::process] read 680368 sequences (100000078 bp) in 1.340 CPU sec, 1.336 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 278742, 7, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 237, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (241.66, 90.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 734)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 338, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (352.66, 167.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 310, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (290.56, 124.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681924 reads in 149.247 CPU sec, 4.754 real sec
sorting block 15, #reads = 1069413 mem = 536877067 peak = 998043648
[M::mem_process_seqs] Processed 682700 reads in 96.870 CPU sec, 3.090 real sec
sorting block 16, #reads = 1069070 mem = 536878692 peak = 1054904320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 278440, 1, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 194, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (266.76, 158.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1067)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 347, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (359.06, 168.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 295, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (270.45, 108.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681966 sequences (100000008 bp) in 2.166 CPU sec, 2.285 real sec...
[M::process] read 681748 sequences (100000130 bp) in 1.320 CPU sec, 1.323 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 278061, 6, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 274, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (259.67, 86.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 359, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (370.21, 172.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 305, 921)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2311)
[M::mem_pestat] mean and std.dev: (523.49, 452.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3006)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681656 reads in 145.558 CPU sec, 4.630 real sec
[M::mem_process_seqs] Processed 680368 reads in 94.017 CPU sec, 2.990 real sec
sorting block 17, #reads = 1069874 mem = 536878848 peak = 1054904320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 277806, 11, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 166, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602)
[M::mem_pestat] mean and std.dev: (205.21, 97.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 346, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (359.60, 171.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (240, 437, 8589)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 25287)
[M::mem_pestat] mean and std.dev: (3369.82, 3949.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 33636)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 322, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1094)
[M::mem_pestat] mean and std.dev: (408.33, 251.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1416)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 680762 sequences (100000176 bp) in 2.195 CPU sec, 2.343 real sec...
[M::process] read 680368 sequences (100000167 bp) in 1.320 CPU sec, 1.324 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 278026, 3, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (245, 333, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (296.83, 104.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 346, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (360.15, 171.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 270, 649)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1505)
[M::mem_pestat] mean and std.dev: (394.41, 250.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1933)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681966 reads in 147.181 CPU sec, 4.677 real sec
[M::mem_process_seqs] Processed 681748 reads in 94.896 CPU sec, 3.015 real sec
sorting block 18, #reads = 1071015 mem = 536877662 peak = 1054904320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 277740, 11, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 250, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (240.70, 125.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 353, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (366.37, 172.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (622, 622, 1319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2713)
[M::mem_pestat] mean and std.dev: (772.80, 409.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3410)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 296, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (322.81, 151.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 680792 sequences (100000074 bp) in 2.326 CPU sec, 2.484 real sec...
[M::process] read 681264 sequences (100000057 bp) in 1.300 CPU sec, 1.298 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 277678, 3, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (252, 318, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (343.21, 148.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 353, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (366.63, 172.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 206, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587)
[M::mem_pestat] mean and std.dev: (234.74, 101.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 725)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680762 reads in 147.904 CPU sec, 4.705 real sec
sorting block 19, #reads = 1070258 mem = 536877723 peak = 1054904320
[M::mem_process_seqs] Processed 680368 reads in 96.024 CPU sec, 3.053 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 277213, 0, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 233, 550)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1380)
[M::mem_pestat] mean and std.dev: (320.71, 274.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1795)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 348, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (363.76, 173.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 290, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (282.78, 112.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 889)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 20, #reads = 1069964 mem = 536877889 peak = 1054904320
[M::process] read 681630 sequences (100000257 bp) in 2.343 CPU sec, 2.517 real sec...
[M::process] read 681270 sequences (100000257 bp) in 1.330 CPU sec, 1.333 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 277403, 3, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 232, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (244.15, 114.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 342, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (358.50, 171.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 303, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (305.48, 165.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680792 reads in 151.263 CPU sec, 4.811 real sec
[M::mem_process_seqs] Processed 681264 reads in 97.724 CPU sec, 3.106 real sec
sorting block 21, #reads = 1070366 mem = 536881993 peak = 1054904320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 277677, 2, 50)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 195, 240)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430)
[M::mem_pestat] mean and std.dev: (186.24, 68.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 525)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 341, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (358.86, 172.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 314, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881)
[M::mem_pestat] mean and std.dev: (300.15, 148.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681050 sequences (100000170 bp) in 2.196 CPU sec, 2.307 real sec...
[M::process] read 681492 sequences (100000224 bp) in 1.290 CPU sec, 1.292 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 277456, 7, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 268, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862)
[M::mem_pestat] mean and std.dev: (265.85, 163.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 343, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (360.77, 173.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 325, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (352.97, 163.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681630 reads in 148.313 CPU sec, 4.716 real sec
[M::mem_process_seqs] Processed 681270 reads in 94.875 CPU sec, 3.017 real sec
sorting block 22, #reads = 1071394 mem = 536879070 peak = 1054904320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 278438, 5, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 312, 822)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2122)
[M::mem_pestat] mean and std.dev: (380.43, 282.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2772)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 344, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (362.50, 175.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 288, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (321.07, 171.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680340 sequences (100000137 bp) in 2.244 CPU sec, 2.387 real sec...
[M::process] read 680954 sequences (100000126 bp) in 1.300 CPU sec, 1.299 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 277958, 10, 61)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 209, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (236.42, 105.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 343, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (358.76, 171.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (292, 710, 996)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2404)
[M::mem_pestat] mean and std.dev: (642.50, 315.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3108)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 326, 640)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1442)
[M::mem_pestat] mean and std.dev: (448.46, 318.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1843)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681050 reads in 147.590 CPU sec, 4.693 real sec
sorting block 23, #reads = 1070867 mem = 536879581 peak = 1054904320
[M::mem_process_seqs] Processed 681492 reads in 95.695 CPU sec, 3.044 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 277401, 11, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 270, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (275.47, 120.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 352, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (364.28, 168.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2342, 2342, 2342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2342, 2342)
[M::mem_pestat] mean and std.dev: (2342.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (2342, 2342)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 267, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (330.86, 193.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 679762 sequences (100000293 bp) in 2.088 CPU sec, 2.256 real sec...
[M::process] read 682120 sequences (100000129 bp) in 1.290 CPU sec, 1.283 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 278080, 7, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 368, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1118)
[M::mem_pestat] mean and std.dev: (333.05, 149.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1426)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 345, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (358.09, 167.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 288, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (324.39, 160.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680340 reads in 146.145 CPU sec, 4.655 real sec
sorting block 24, #reads = 1069738 mem = 536880937 peak = 1054904320
[M::mem_process_seqs] Processed 680954 reads in 94.301 CPU sec, 3.008 real sec
sorting block 25, #reads = 1071537 mem = 536877513 peak = 1103470592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 276788, 5, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (246, 322, 574)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1230)
[M::mem_pestat] mean and std.dev: (325.29, 180.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1558)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 358, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (370.04, 171.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 371, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1085)
[M::mem_pestat] mean and std.dev: (349.73, 200.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1392)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682504 sequences (100000010 bp) in 2.076 CPU sec, 2.220 real sec...
[M::process] read 681120 sequences (100000076 bp) in 1.320 CPU sec, 1.324 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 277644, 5, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 232, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544)
[M::mem_pestat] mean and std.dev: (243.07, 82.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 661)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 344, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (358.24, 170.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 320, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (343.61, 134.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 679762 reads in 148.638 CPU sec, 4.732 real sec
[M::mem_process_seqs] Processed 682120 reads in 96.127 CPU sec, 3.064 real sec
sorting block 26, #reads = 1070526 mem = 536877435 peak = 1103470592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 278231, 10, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (271, 317, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (343.12, 149.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 340, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (354.95, 169.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (43, 505, 1023)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2983)
[M::mem_pestat] mean and std.dev: (554.40, 579.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3963)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 240, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (232.02, 93.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 889)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 681132 sequences (100000238 bp) in 2.228 CPU sec, 2.365 real sec...
[M::process] read 680732 sequences (100000050 bp) in 1.290 CPU sec, 1.290 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 277552, 6, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 242, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (260.62, 95.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 350, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (363.44, 171.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 300, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (309.05, 155.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1080)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682504 reads in 151.661 CPU sec, 4.824 real sec
sorting block 27, #reads = 1071193 mem = 536878438 peak = 1103470592
[M::process] read 681284 sequences (100000060 bp) in 1.573 CPU sec, 1.574 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 277194, 7, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 229, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586)
[M::mem_pestat] mean and std.dev: (247.88, 121.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 734)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 348, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (362.64, 171.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (275, 301, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (153, 458)
[M::mem_pestat] mean and std.dev: (306.62, 63.72)
[M::mem_pestat] low and high boundaries for proper pairs: (52, 561)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681132 reads in 90.926 CPU sec, 3.185 real sec
[M::mem_process_seqs] Processed 681120 reads in 207.727 CPU sec, 6.913 real sec
sorting block 28, #reads = 1070182 mem = 536878259 peak = 1103470592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 276869, 5, 53)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 286, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (298.29, 149.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 353, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (366.48, 172.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 260, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (257.24, 114.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681910 sequences (100000058 bp) in 2.096 CPU sec, 2.242 real sec...
[M::process] read 681590 sequences (100000158 bp) in 1.319 CPU sec, 1.318 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 276778, 2, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 232, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866)
[M::mem_pestat] mean and std.dev: (252.42, 126.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 344, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (359.91, 170.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 333, 604)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1388)
[M::mem_pestat] mean and std.dev: (425.39, 341.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1790)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680732 reads in 170.992 CPU sec, 5.436 real sec
[M::mem_process_seqs] Processed 681284 reads in 101.326 CPU sec, 3.215 real sec
sorting block 29, #reads = 1069765 mem = 536879695 peak = 1103470592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 277163, 4, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (229, 267, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (299.26, 146.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 897)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 339, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (354.92, 169.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 247, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1213)
[M::mem_pestat] mean and std.dev: (343.83, 251.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1555)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681534 sequences (100000184 bp) in 1.911 CPU sec, 2.047 real sec...
[M::process] read 684098 sequences (100000110 bp) in 1.380 CPU sec, 1.375 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 277573, 4, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 248, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583)
[M::mem_pestat] mean and std.dev: (262.83, 108.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 714)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 341, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (358.33, 171.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 246, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (251.62, 70.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 683)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681910 reads in 154.148 CPU sec, 4.914 real sec
sorting block 30, #reads = 1068453 mem = 536879046 peak = 1103470592
[M::mem_process_seqs] Processed 681590 reads in 98.797 CPU sec, 3.142 real sec
sorting block 31, #reads = 1068302 mem = 536880392 peak = 1103470592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 277847, 13, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (254, 442, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1082)
[M::mem_pestat] mean and std.dev: (329.09, 142.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1358)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 342, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (360.19, 173.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (316, 426, 1315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3313)
[M::mem_pestat] mean and std.dev: (779.54, 726.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4312)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (135, 232, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (257.54, 142.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 683612 sequences (100000223 bp) in 2.057 CPU sec, 2.185 real sec...
[M::process] read 684466 sequences (100000090 bp) in 1.410 CPU sec, 1.416 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 279988, 9, 48)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 218, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (221.19, 93.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 336, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (356.85, 177.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 263, 697)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1737)
[M::mem_pestat] mean and std.dev: (396.23, 303.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2257)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681534 reads in 152.642 CPU sec, 4.850 real sec
[M::mem_process_seqs] Processed 684098 reads in 100.826 CPU sec, 3.204 real sec
sorting block 32, #reads = 1069124 mem = 536878438 peak = 1103470592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 279289, 6, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (111, 150, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (193.69, 107.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 339, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (357.32, 176.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 357, 582)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1376)
[M::mem_pestat] mean and std.dev: (356.97, 236.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1773)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682960 sequences (100000038 bp) in 2.070 CPU sec, 2.224 real sec...
[M::process] read 684796 sequences (100000029 bp) in 1.390 CPU sec, 1.393 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 280068, 8, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (230, 367, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (343.59, 152.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 332, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (352.24, 174.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 234, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (244.03, 118.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683612 reads in 155.547 CPU sec, 4.953 real sec
[M::mem_process_seqs] Processed 684466 reads in 100.089 CPU sec, 3.180 real sec
sorting block 33, #reads = 1067688 mem = 536880941 peak = 1103470592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 279150, 1, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 312, 983)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2549)
[M::mem_pestat] mean and std.dev: (494.40, 372.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3332)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 343, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (359.22, 174.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 459, 541)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1187)
[M::mem_pestat] mean and std.dev: (388.46, 196.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1510)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681710 sequences (100000162 bp) in 2.214 CPU sec, 2.313 real sec...
[M::process] read 682010 sequences (100000067 bp) in 1.370 CPU sec, 1.374 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 279974, 13, 52)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (222, 245, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (295.67, 140.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 330, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (348.81, 171.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (345, 974, 974)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2232)
[M::mem_pestat] mean and std.dev: (726.82, 331.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2861)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 294, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (275.72, 89.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682960 reads in 157.434 CPU sec, 5.010 real sec
[M::mem_process_seqs] Processed 684796 reads in 102.125 CPU sec, 3.254 real sec
sorting block 34, #reads = 1068201 mem = 536878912 peak = 1103470592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 278728, 7, 50)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (248, 386, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (334.47, 125.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 351, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (365.72, 175.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1291)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 309, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1179)
[M::mem_pestat] mean and std.dev: (375.18, 245.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1510)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681782 sequences (100000043 bp) in 2.044 CPU sec, 2.189 real sec...
[M::process] read 680722 sequences (100000176 bp) in 1.370 CPU sec, 1.363 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 278795, 11, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (231, 291, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690)
[M::mem_pestat] mean and std.dev: (293.29, 117.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 346, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (360.96, 173.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 842, 1353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4027)
[M::mem_pestat] mean and std.dev: (685.00, 604.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5364)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 325, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (385.54, 230.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1315)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681710 reads in 159.349 CPU sec, 5.077 real sec
sorting block 35, #reads = 1067901 mem = 536882733 peak = 1103470592
[M::mem_process_seqs] Processed 682010 reads in 102.821 CPU sec, 3.273 real sec
sorting block 36, #reads = 1064853 mem = 536878037 peak = 1125982208
[M::process] read 681534 sequences (100000153 bp) in 2.000 CPU sec, 2.090 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 277693, 5, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 278, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (257.23, 111.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 342, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (357.27, 169.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 325, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (308.18, 104.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681552 sequences (100000278 bp) in 1.380 CPU sec, 1.379 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 277831, 3, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 372, 713)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1813)
[M::mem_pestat] mean and std.dev: (517.28, 522.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2607)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 352, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (366.15, 173.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 303, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053)
[M::mem_pestat] mean and std.dev: (322.41, 160.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1334)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 277854, 6, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 238, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (223.48, 83.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 342, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (358.61, 171.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 260, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (274.13, 117.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 876)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681782 reads in 229.559 CPU sec, 7.335 real sec
[M::mem_process_seqs] Processed 680722 reads in 172.512 CPU sec, 5.526 real sec
[M::mem_process_seqs] Processed 681534 reads in 189.158 CPU sec, 6.089 real sec
sorting block 37, #reads = 1065925 mem = 536878255 peak = 1125982208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 277568, 27, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 185, 286)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572)
[M::mem_pestat] mean and std.dev: (197.60, 79.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 715)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 682802 sequences (100000231 bp) in 2.210 CPU sec, 2.427 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (222, 346, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (362.97, 174.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (314, 889, 2518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6926)
[M::mem_pestat] mean and std.dev: (1995.70, 1995.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9979)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 300, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (288.41, 139.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 38, #reads = 1065565 mem = 536882592 peak = 1125982208
[M::process] read 686838 sequences (100000207 bp) in 1.794 CPU sec, 1.887 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 279539, 9, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 259, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (241.09, 108.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 342, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (359.77, 175.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 299, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (330.79, 134.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681552 reads in 162.708 CPU sec, 5.236 real sec
[M::mem_process_seqs] Processed 682802 reads in 107.013 CPU sec, 3.381 real sec
[M::process] read 685416 sequences (100000294 bp) in 1.774 CPU sec, 1.854 real sec...
sorting block 39, #reads = 1066201 mem = 536886593 peak = 1125982208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 280980, 9, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 244, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (240.72, 106.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 757)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 319, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (336.21, 164.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 313, 849)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2251)
[M::mem_pestat] mean and std.dev: (429.61, 460.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2952)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684188 sequences (100000270 bp) in 2.237 CPU sec, 2.408 real sec...
[M::process] read 685100 sequences (100000016 bp) in 1.310 CPU sec, 1.305 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 280250, 4, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 225, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1239)
[M::mem_pestat] mean and std.dev: (270.71, 197.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1598)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 325, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (341.95, 166.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 368, 631)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1395)
[M::mem_pestat] mean and std.dev: (378.42, 218.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1777)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686838 reads in 171.819 CPU sec, 5.475 real sec
[M::mem_process_seqs] Processed 685416 reads in 109.385 CPU sec, 3.484 real sec
sorting block 40, #reads = 1061709 mem = 536880362 peak = 1125982208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 280301, 2, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 179, 812)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2142)
[M::mem_pestat] mean and std.dev: (453.54, 438.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2807)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 333, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (348.81, 168.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 316, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (326.00, 155.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682488 sequences (100000152 bp) in 2.196 CPU sec, 2.319 real sec...
[M::process] read 682908 sequences (100000083 bp) in 1.380 CPU sec, 1.380 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 280373, 4, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 295, 782)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1982)
[M::mem_pestat] mean and std.dev: (452.69, 391.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2582)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 326, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (343.97, 168.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 386, 590)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1344)
[M::mem_pestat] mean and std.dev: (371.47, 198.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1721)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684188 reads in 158.991 CPU sec, 5.056 real sec
sorting block 41, #reads = 1063990 mem = 536879628 peak = 1125982208
[M::mem_process_seqs] Processed 685100 reads in 103.284 CPU sec, 3.292 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 279030, 3, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (230, 293, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (291.13, 102.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 345, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (360.22, 173.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 233, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (308.35, 208.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682064 sequences (100000209 bp) in 2.061 CPU sec, 2.200 real sec...
[M::process] read 680608 sequences (100000293 bp) in 1.340 CPU sec, 1.340 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 279326, 5, 48)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 263, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1073)
[M::mem_pestat] mean and std.dev: (285.21, 144.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1380)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 343, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (359.67, 174.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 233, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524)
[M::mem_pestat] mean and std.dev: (238.00, 84.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 639)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682488 reads in 163.929 CPU sec, 5.463 real sec
sorting block 42, #reads = 1065778 mem = 536878718 peak = 1125982208
[M::mem_process_seqs] Processed 682908 reads in 103.092 CPU sec, 3.284 real sec
sorting block 43, #reads = 1064877 mem = 536880069 peak = 1147334656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 278944, 3, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 339, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068)
[M::mem_pestat] mean and std.dev: (332.54, 193.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1369)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 346, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (361.87, 174.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 263, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (243.65, 117.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 959)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682118 sequences (100000090 bp) in 1.955 CPU sec, 2.077 real sec...
[M::process] read 681880 sequences (100000295 bp) in 1.400 CPU sec, 1.397 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 278204, 6, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 212, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (271.46, 156.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 356, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (371.53, 178.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 320, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881)
[M::mem_pestat] mean and std.dev: (323.03, 108.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1085)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682064 reads in 159.783 CPU sec, 5.166 real sec
[M::mem_process_seqs] Processed 680608 reads in 103.111 CPU sec, 3.289 real sec
sorting block 44, #reads = 1065269 mem = 536878726 peak = 1147334656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278899, 16, 61)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (221, 379, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (334.42, 119.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 344, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (362.24, 176.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 855, 1497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4115)
[M::mem_pestat] mean and std.dev: (852.67, 800.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5424)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 471, 779)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1839)
[M::mem_pestat] mean and std.dev: (469.36, 274.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2369)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 683646 sequences (100000098 bp) in 2.026 CPU sec, 2.158 real sec...
[M::process] read 684540 sequences (100000234 bp) in 1.360 CPU sec, 1.364 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 279002, 4, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 319, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1184)
[M::mem_pestat] mean and std.dev: (330.51, 228.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1520)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 348, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (367.87, 181.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1306)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 419, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1089)
[M::mem_pestat] mean and std.dev: (328.03, 171.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1381)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682118 reads in 157.291 CPU sec, 5.006 real sec
[M::mem_process_seqs] Processed 681880 reads in 101.792 CPU sec, 3.243 real sec
sorting block 45, #reads = 1065276 mem = 536878889 peak = 1147334656
[M::process] read 683348 sequences (100000068 bp) in 1.961 CPU sec, 2.067 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 279229, 5, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 248, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (268.16, 126.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 337, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (356.16, 175.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 387, 544)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1172)
[M::mem_pestat] mean and std.dev: (335.32, 149.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1486)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682358 sequences (100000107 bp) in 1.360 CPU sec, 1.363 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 280244, 10, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 261, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (253.00, 146.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 333, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (349.26, 169.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (334, 873, 960)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2212)
[M::mem_pestat] mean and std.dev: (651.62, 334.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2838)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 301, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (284.21, 128.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 279803, 11, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 286, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1146)
[M::mem_pestat] mean and std.dev: (317.68, 192.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1462)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 341, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (356.13, 171.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 2248, 2248)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6390)
[M::mem_pestat] mean and std.dev: (1343.55, 1015.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8461)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 369, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (347.52, 163.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683646 reads in 229.139 CPU sec, 7.525 real sec
sorting block 46, #reads = 1065878 mem = 536879945 peak = 1147334656
[M::mem_process_seqs] Processed 684540 reads in 166.474 CPU sec, 5.341 real sec
[M::mem_process_seqs] Processed 683348 reads in 181.910 CPU sec, 5.858 real sec
sorting block 47, #reads = 1063721 mem = 536880272 peak = 1153224704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 279053, 3, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 221, 593)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1475)
[M::mem_pestat] mean and std.dev: (321.03, 209.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1916)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 345, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (357.85, 169.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 282, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (291.94, 144.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684624 sequences (100000052 bp) in 2.400 CPU sec, 2.584 real sec...
[M::mem_process_seqs] Processed 682358 reads in 85.466 CPU sec, 2.821 real sec
sorting block 48, #reads = 1069340 mem = 536881039 peak = 1153224704
[M::process] read 683860 sequences (100000068 bp) in 2.227 CPU sec, 2.472 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 280252, 5, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 398, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (334.34, 161.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 325, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (340.85, 163.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 289, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (334.00, 154.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681812 sequences (100000007 bp) in 1.573 CPU sec, 1.611 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 279506, 4, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 212, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (256.52, 160.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1337)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 333, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (347.99, 167.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 203, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (248.37, 108.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683400 sequences (100000183 bp) in 1.320 CPU sec, 1.323 real sec...
[M::mem_process_seqs] Processed 684624 reads in 157.976 CPU sec, 5.011 real sec
[M::mem_process_seqs] Processed 683860 reads in 100.386 CPU sec, 3.174 real sec
sorting block 49, #reads = 1069058 mem = 536877664 peak = 1153224704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 278489, 1, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 253, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (222.25, 80.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 346, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (359.45, 170.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 205, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543)
[M::mem_pestat] mean and std.dev: (238.47, 104.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 666)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682874 sequences (100000029 bp) in 2.014 CPU sec, 2.144 real sec...
[M::process] read 682534 sequences (100000131 bp) in 1.360 CPU sec, 1.357 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 278743, 5, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (203, 243, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (265.89, 93.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 335, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (350.05, 168.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 343, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1058)
[M::mem_pestat] mean and std.dev: (333.39, 181.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1334)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681812 reads in 157.892 CPU sec, 5.091 real sec
sorting block 50, #reads = 1068160 mem = 536879416 peak = 1153224704
[M::mem_process_seqs] Processed 683400 reads in 99.077 CPU sec, 3.264 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 278888, 5, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (212, 301, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (285.46, 119.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 340, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (355.48, 170.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 254, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684)
[M::mem_pestat] mean and std.dev: (278.85, 103.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682608 sequences (100000290 bp) in 1.974 CPU sec, 2.116 real sec...
[M::process] read 683544 sequences (100000058 bp) in 1.370 CPU sec, 1.368 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 278818, 2, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 217, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (248.43, 132.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 341, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (358.05, 172.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 311, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (335.54, 146.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1301)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682874 reads in 158.641 CPU sec, 5.148 real sec
sorting block 51, #reads = 1067791 mem = 536883628 peak = 1174872064
[M::mem_process_seqs] Processed 682534 reads in 102.807 CPU sec, 3.279 real sec
sorting block 52, #reads = 1068187 mem = 536877867 peak = 1175130112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 279155, 12, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (112, 225, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1096)
[M::mem_pestat] mean and std.dev: (268.14, 176.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1424)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 342, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (359.41, 174.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (653, 1846, 2107)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5015)
[M::mem_pestat] mean and std.dev: (1093.70, 748.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6469)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 259, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (233.55, 92.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 686668 sequences (100000086 bp) in 1.963 CPU sec, 2.102 real sec...
[M::process] read 686772 sequences (100000135 bp) in 1.460 CPU sec, 1.452 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 279800, 6, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (212, 307, 583)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1325)
[M::mem_pestat] mean and std.dev: (336.33, 239.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1696)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 333, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (353.64, 175.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 310, 591)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1357)
[M::mem_pestat] mean and std.dev: (388.08, 279.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1740)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682608 reads in 162.492 CPU sec, 5.261 real sec
[M::mem_process_seqs] Processed 683544 reads in 106.750 CPU sec, 3.401 real sec
sorting block 53, #reads = 1065364 mem = 536881878 peak = 1175130112
[M::process] read 685394 sequences (100000112 bp) in 1.923 CPU sec, 2.025 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 280113, 7, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (125, 176, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (219.02, 130.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 321, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (337.38, 164.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 294, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (281.14, 109.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687288 sequences (100000056 bp) in 1.420 CPU sec, 1.419 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 280889, 7, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 196, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (267.55, 139.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 316, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (331.33, 158.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 284, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (264.09, 98.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 280114, 4, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 256, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (249.20, 127.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 331, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (343.64, 164.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 253, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (374.26, 259.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1410)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686668 reads in 248.293 CPU sec, 8.026 real sec
sorting block 54, #reads = 1060679 mem = 536879639 peak = 1175130112
[M::mem_process_seqs] Processed 686772 reads in 184.558 CPU sec, 5.935 real sec
[M::mem_process_seqs] Processed 685394 reads in 202.630 CPU sec, 6.534 real sec
sorting block 55, #reads = 1060875 mem = 536879052 peak = 1175130112
[M::process] read 685442 sequences (100000145 bp) in 2.175 CPU sec, 2.351 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 281070, 6, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 213, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705)
[M::mem_pestat] mean and std.dev: (245.14, 134.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 889)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 311, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (328.42, 159.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 263, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (266.58, 113.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683912 sequences (100000153 bp) in 1.819 CPU sec, 1.916 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 280079, 4, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 245, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1149)
[M::mem_pestat] mean and std.dev: (249.62, 133.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1475)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 322, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (336.77, 160.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (129, 229, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (233.74, 122.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 761)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683806 sequences (100000167 bp) in 1.330 CPU sec, 1.339 real sec...
[M::mem_process_seqs] Processed 687288 reads in 168.237 CPU sec, 5.363 real sec
[M::mem_process_seqs] Processed 685442 reads in 108.232 CPU sec, 3.616 real sec
sorting block 56, #reads = 1064983 mem = 536880617 peak = 1175130112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 279318, 3, 50)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (201, 297, 578)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1332)
[M::mem_pestat] mean and std.dev: (304.90, 161.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1709)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 331, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (344.44, 162.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 324, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (331.56, 173.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683718 sequences (100000129 bp) in 2.107 CPU sec, 2.273 real sec...
[M::process] read 683426 sequences (100000257 bp) in 1.330 CPU sec, 1.335 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 278997, 3, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 199, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (201.00, 89.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 330, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (343.97, 162.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 259, 1245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3403)
[M::mem_pestat] mean and std.dev: (546.43, 549.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4482)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683912 reads in 165.684 CPU sec, 5.467 real sec
sorting block 57, #reads = 1065286 mem = 536879488 peak = 1175130112
[M::mem_process_seqs] Processed 683806 reads in 103.630 CPU sec, 3.354 real sec
sorting block 58, #reads = 1066347 mem = 536877238 peak = 1175130112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 278755, 10, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 216, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1239)
[M::mem_pestat] mean and std.dev: (223.52, 109.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1605)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 332, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (346.57, 164.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 181, 1054)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2900)
[M::mem_pestat] mean and std.dev: (349.89, 381.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3823)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 273, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (270.37, 93.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 682990 sequences (100000293 bp) in 2.037 CPU sec, 2.181 real sec...
[M::process] read 497840 sequences (72897995 bp) in 1.100 CPU sec, 1.104 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 278828, 10, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 241, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (254.07, 125.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 857)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 332, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (348.19, 167.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (403, 955, 1503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3703)
[M::mem_pestat] mean and std.dev: (1003.40, 660.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4803)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 206, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (233.93, 111.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683718 reads in 164.950 CPU sec, 5.315 real sec
[M::mem_process_seqs] Processed 683426 reads in 103.762 CPU sec, 3.422 real sec
sorting block 59, #reads = 1065821 mem = 536878542 peak = 1175130112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 278569, 7, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 180, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1130)
[M::mem_pestat] mean and std.dev: (269.07, 174.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1463)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 337, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (353.63, 170.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 250, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (244.22, 115.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 203325, 5, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (238, 302, 844)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2056)
[M::mem_pestat] mean and std.dev: (481.59, 365.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2662)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 336, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (354.20, 172.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 294, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (245.54, 121.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1322)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682990 reads in 138.189 CPU sec, 4.579 real sec
[M::mem_process_seqs] Processed 497840 reads in 78.826 CPU sec, 2.578 real sec
sorting block 60, #reads = 1067065 mem = 536877928 peak = 1175130112
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 65310394
overall: 70173417472 mem 9529.837 user 346.012 sys 1594.745 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1029_N5D-T1-TDNA-1_A23MHWWLT4_1.L008\tPU:L008\tSM:1029_N5D-T1-TDNA-1_A23MHWWLT4_1\tLB:1029_N5D-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0002.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0002.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 1594.783 sec; CPU: 9875.852 sec
sorting block 61, #reads = 532308 mem = 267902522 peak = 1175130112
uniq header count 1
merging 62 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3973365862
output reads: 65693311
bam_mem_sort: 62 calls 22.798 user 0.176 sys 23.111 real
bam_write: 62 calls 49.803 user 0.943 sys 51.098 real
execute: 1 calls 12.136 user 0.781 sys 1617.126 real
merge_files: 1 calls 12.119 user 0.755 sys 21.566 real
parse_chunk: 984 calls 30.225 user 8.970 sys 39.867 real
read_chunk: 2658 calls 26.321 user 24.545 sys 272.799 real
sort_block: 1 calls 20.177 user 24.072 sys 264.002 real
write_chunk: 1672 calls 11.824 user 0.551 sys 20.851 real
overall: 2906095616 mem 810.099 user 42.373 sys 1617.148 real
ls: cannot access '*.cram': No such file or directory