Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/92/b72bf64435ce078778748c20d26323/1136_3U6-T1-TDNA-1_A23MHWWLT4_1/0001.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/27/d64f238e9bbb689492f100167f3603/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/92/b72bf64435ce078778748c20d26323/1136_3U6-T1-TDNA-1_A23MHWWLT4_1/0001.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/27/d64f238e9bbb689492f100167f3603/.command.run
==> STAGING COMPLETE (7 inputs)

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_3U6-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1689.122 CPU sec, 180.174 real sec
[M::process] read 686728 sequences (100000212 bp) in 0.988 CPU sec, 0.991 real sec...
[M::process] read 685026 sequences (100000237 bp) in 1.197 CPU sec, 1.246 real sec...
[M::process] read 684016 sequences (100000299 bp) in 1.228 CPU sec, 1.223 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 284433, 4, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (122, 199, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (238.33, 160.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 299, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (318.84, 157.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 345, 2026)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5580)
[M::mem_pestat] mean and std.dev: (1047.46, 1056.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7357)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683258 sequences (100000130 bp) in 1.149 CPU sec, 1.157 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 283034, 4, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (263, 308, 645)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1409)
[M::mem_pestat] mean and std.dev: (432.04, 304.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1791)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 310, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (328.20, 161.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 382, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (334.52, 135.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 283457, 4, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (259, 481, 612)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1318)
[M::mem_pestat] mean and std.dev: (367.25, 170.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1671)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 317, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (334.26, 163.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 528, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1246)
[M::mem_pestat] mean and std.dev: (509.21, 364.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1968)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686728 reads in 233.117 CPU sec, 7.488 real sec
[M::mem_process_seqs] Processed 685026 reads in 148.526 CPU sec, 4.828 real sec
[M::mem_process_seqs] Processed 684016 reads in 160.437 CPU sec, 5.237 real sec
sorting block 0, #reads = 1081780 mem = 536882184 peak = 587227136
[M::process] read 682924 sequences (100000248 bp) in 1.783 CPU sec, 1.945 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282972, 3, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 249, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696)
[M::mem_pestat] mean and std.dev: (242.50, 108.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 869)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 322, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (338.74, 165.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 273, 1258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3442)
[M::mem_pestat] mean and std.dev: (726.62, 929.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4534)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683074 sequences (100000052 bp) in 1.297 CPU sec, 1.311 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282713, 9, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 304, 680)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1690)
[M::mem_pestat] mean and std.dev: (401.56, 298.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2195)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 325, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (341.01, 166.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 321, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699)
[M::mem_pestat] mean and std.dev: (291.25, 72.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683142 sequences (100000042 bp) in 1.190 CPU sec, 1.188 real sec...
[M::mem_process_seqs] Processed 683258 reads in 141.227 CPU sec, 4.497 real sec
sorting block 1, #reads = 1081422 mem = 536877173 peak = 805855232
[M::mem_process_seqs] Processed 682924 reads in 88.104 CPU sec, 2.790 real sec
sorting block 2, #reads = 1080950 mem = 536881244 peak = 904589312
[M::process] read 682568 sequences (100000264 bp) in 1.633 CPU sec, 1.738 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 281865, 5, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (243, 396, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (373.88, 159.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 321, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (338.32, 165.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 287, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512)
[M::mem_pestat] mean and std.dev: (234.82, 67.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 615)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682014 sequences (100000198 bp) in 1.270 CPU sec, 1.275 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 282852, 1, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (224, 286, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (321.59, 151.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 319, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (335.91, 163.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 318, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (291.87, 95.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683074 reads in 141.696 CPU sec, 4.525 real sec
[M::mem_process_seqs] Processed 683142 reads in 87.995 CPU sec, 2.813 real sec
sorting block 3, #reads = 1079243 mem = 536879048 peak = 904589312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 282412, 0, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 218, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439)
[M::mem_pestat] mean and std.dev: (194.16, 67.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 531)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 321, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (338.58, 164.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (137, 241, 629)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1613)
[M::mem_pestat] mean and std.dev: (371.04, 312.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2105)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681754 sequences (100000224 bp) in 1.892 CPU sec, 1.990 real sec...
[M::process] read 681354 sequences (100000027 bp) in 1.280 CPU sec, 1.285 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 281346, 1, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 389, 1013)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2705)
[M::mem_pestat] mean and std.dev: (490.47, 380.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3551)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 326, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (341.07, 163.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 198, 617)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1547)
[M::mem_pestat] mean and std.dev: (313.15, 207.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2012)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682568 reads in 143.195 CPU sec, 4.571 real sec
[M::mem_process_seqs] Processed 682014 reads in 88.547 CPU sec, 2.830 real sec
sorting block 4, #reads = 1079859 mem = 536877779 peak = 904589312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 281431, 2, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 334, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (289.88, 110.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 324, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (339.71, 162.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 298, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (311.36, 130.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681000 sequences (100000066 bp) in 1.844 CPU sec, 1.988 real sec...
[M::process] read 686176 sequences (100000163 bp) in 1.330 CPU sec, 1.328 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 282290, 3, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (230, 399, 711)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1673)
[M::mem_pestat] mean and std.dev: (473.00, 376.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2154)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 327, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (342.21, 163.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 397, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (307.13, 145.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1327)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681754 reads in 141.484 CPU sec, 4.522 real sec
[M::mem_process_seqs] Processed 681354 reads in 87.313 CPU sec, 2.783 real sec
sorting block 5, #reads = 1079644 mem = 536878850 peak = 904589312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282040, 4, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 343, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045)
[M::mem_pestat] mean and std.dev: (258.92, 121.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1330)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 332, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (347.17, 166.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 337, 768)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1882)
[M::mem_pestat] mean and std.dev: (381.50, 251.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2439)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684862 sequences (100000195 bp) in 2.145 CPU sec, 2.279 real sec...
[M::process] read 684050 sequences (100000131 bp) in 1.350 CPU sec, 1.352 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283404, 8, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 220, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (272.78, 132.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 305, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (325.45, 162.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 392, 1010)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2614)
[M::mem_pestat] mean and std.dev: (512.18, 348.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3416)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681000 reads in 141.404 CPU sec, 4.512 real sec
sorting block 6, #reads = 1079587 mem = 536879952 peak = 904589312
[M::mem_process_seqs] Processed 686176 reads in 88.258 CPU sec, 2.808 real sec
sorting block 7, #reads = 1080705 mem = 536878682 peak = 933191680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283655, 4, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (284, 363, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (349.67, 120.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 315, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (333.10, 165.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 393, 886)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2162)
[M::mem_pestat] mean and std.dev: (544.00, 501.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2800)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683432 sequences (100000228 bp) in 1.815 CPU sec, 1.938 real sec...
[M::process] read 682708 sequences (100000066 bp) in 1.340 CPU sec, 1.338 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 283294, 13, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 278, 1016)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2682)
[M::mem_pestat] mean and std.dev: (559.31, 569.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3515)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 321, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (337.55, 165.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (340, 456, 1053)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2479)
[M::mem_pestat] mean and std.dev: (547.00, 535.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3192)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 276, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (66, 511)
[M::mem_pestat] mean and std.dev: (247.05, 79.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 600)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684862 reads in 140.015 CPU sec, 4.481 real sec
[M::mem_process_seqs] Processed 684050 reads in 86.791 CPU sec, 2.768 real sec
sorting block 8, #reads = 1082796 mem = 536877346 peak = 933191680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 282851, 3, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 250, 589)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1469)
[M::mem_pestat] mean and std.dev: (327.77, 242.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1909)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 324, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (340.35, 166.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 407, 901)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2223)
[M::mem_pestat] mean and std.dev: (513.38, 296.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2884)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682768 sequences (100000074 bp) in 2.116 CPU sec, 2.225 real sec...
[M::process] read 683204 sequences (100000147 bp) in 1.360 CPU sec, 1.364 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283059, 3, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 307, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (242.13, 106.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 327, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (344.26, 168.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 289, 703)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1713)
[M::mem_pestat] mean and std.dev: (370.36, 262.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2218)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683432 reads in 140.691 CPU sec, 4.489 real sec
[M::mem_process_seqs] Processed 682708 reads in 87.387 CPU sec, 2.789 real sec
sorting block 9, #reads = 1081860 mem = 536880196 peak = 933191680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282481, 5, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 262, 642)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1620)
[M::mem_pestat] mean and std.dev: (421.82, 375.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2109)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 323, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (340.30, 166.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 263, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611)
[M::mem_pestat] mean and std.dev: (270.59, 115.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 739)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683188 sequences (100000123 bp) in 2.004 CPU sec, 2.148 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283167, 13, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 379, 649)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1647)
[M::mem_pestat] mean and std.dev: (284.21, 176.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2146)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 682458 sequences (100000239 bp) in 1.420 CPU sec, 1.413 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (201, 321, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (339.24, 167.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (350, 998, 7713)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 22439)
[M::mem_pestat] mean and std.dev: (2786.77, 2995.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 29802)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 269, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (257.30, 138.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1094)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682768 reads in 142.042 CPU sec, 4.539 real sec
sorting block 10, #reads = 1081095 mem = 536879628 peak = 933191680
[M::mem_process_seqs] Processed 683204 reads in 88.566 CPU sec, 2.815 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282686, 7, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 201, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (247.52, 119.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 321, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (338.29, 165.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 268, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (29, 584)
[M::mem_pestat] mean and std.dev: (262.60, 71.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 695)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 11, #reads = 1080147 mem = 536876586 peak = 933191680
[M::process] read 682336 sequences (100000160 bp) in 2.219 CPU sec, 2.342 real sec...
[M::process] read 681662 sequences (100000111 bp) in 1.410 CPU sec, 1.411 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282764, 1, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 235, 575)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1411)
[M::mem_pestat] mean and std.dev: (308.75, 190.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1829)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 322, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (339.39, 165.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 275, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765)
[M::mem_pestat] mean and std.dev: (275.60, 140.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 964)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683188 reads in 142.496 CPU sec, 4.548 real sec
[M::mem_process_seqs] Processed 682458 reads in 88.634 CPU sec, 2.819 real sec
sorting block 12, #reads = 1080091 mem = 536879559 peak = 933191680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 282346, 8, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (225, 352, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (381.29, 228.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 326, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (343.04, 167.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 681260 sequences (100000018 bp) in 2.044 CPU sec, 2.171 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282143, 14, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 209, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718)
[M::mem_pestat] mean and std.dev: (231.11, 100.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 327, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (343.11, 165.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (438, 1245, 1617)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3975)
[M::mem_pestat] mean and std.dev: (1028.00, 909.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5154)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 229, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062)
[M::mem_pestat] mean and std.dev: (270.82, 113.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1350)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 686346 sequences (100000196 bp) in 1.450 CPU sec, 1.450 real sec...
[M::mem_process_seqs] Processed 682336 reads in 142.331 CPU sec, 4.548 real sec
[M::mem_process_seqs] Processed 681662 reads in 88.389 CPU sec, 2.808 real sec
sorting block 13, #reads = 1079680 mem = 536879594 peak = 933191680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282445, 7, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 249, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (290.00, 138.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 333, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (349.14, 169.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 284, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 529)
[M::mem_pestat] mean and std.dev: (264.75, 91.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 632)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685114 sequences (100000085 bp) in 2.561 CPU sec, 2.726 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284217, 9, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (255, 315, 2438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6804)
[M::mem_pestat] mean and std.dev: (1140.73, 1482.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8987)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 303, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (321.65, 158.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 215, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (243.78, 103.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681260 reads in 144.527 CPU sec, 4.609 real sec
[M::process] read 683938 sequences (100000211 bp) in 1.685 CPU sec, 1.707 real sec...
[M::mem_process_seqs] Processed 686346 reads in 90.884 CPU sec, 2.873 real sec
sorting block 14, #reads = 1079066 mem = 536878569 peak = 933191680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283887, 11, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (93, 148, 1373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3933)
[M::mem_pestat] mean and std.dev: (497.26, 590.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5213)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 311, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (328.28, 160.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (336, 694, 1608)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4152)
[M::mem_pestat] mean and std.dev: (639.00, 459.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5424)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 271, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (272.62, 97.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 683266 sequences (100000030 bp) in 2.335 CPU sec, 2.515 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 282646, 3, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 193, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (244.88, 120.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 317, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (332.16, 160.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (127, 173, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (195.08, 87.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685114 reads in 146.738 CPU sec, 4.691 real sec
[M::mem_process_seqs] Processed 683938 reads in 91.764 CPU sec, 2.903 real sec
sorting block 15, #reads = 1080659 mem = 536879808 peak = 933191680
[M::process] read 682600 sequences (100000298 bp) in 1.834 CPU sec, 1.875 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282454, 7, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (224, 294, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (287.79, 95.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 321, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (335.94, 162.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (381, 575, 1322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3204)
[M::mem_pestat] mean and std.dev: (693.00, 437.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4145)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 16, #reads = 1081222 mem = 536883755 peak = 933191680
[M::process] read 682574 sequences (100000033 bp) in 2.604 CPU sec, 2.778 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 282082, 2, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 303, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (312.50, 150.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 326, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (340.49, 164.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 438, 760)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1748)
[M::mem_pestat] mean and std.dev: (533.71, 404.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2242)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683266 reads in 149.194 CPU sec, 4.763 real sec
[M::process] read 682882 sequences (100000211 bp) in 1.761 CPU sec, 1.789 real sec...
[M::mem_process_seqs] Processed 682600 reads in 93.613 CPU sec, 2.960 real sec
sorting block 17, #reads = 1080519 mem = 536878642 peak = 933191680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 282645, 5, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 451, 1711)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4763)
[M::mem_pestat] mean and std.dev: (976.14, 1104.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6289)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 325, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (340.63, 164.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (283, 308, 665)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1429)
[M::mem_pestat] mean and std.dev: (427.81, 280.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1811)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682678 sequences (100000049 bp) in 2.413 CPU sec, 2.567 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282523, 6, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (274, 402, 746)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1690)
[M::mem_pestat] mean and std.dev: (456.36, 230.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2162)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 322, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (338.87, 164.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 282, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (388.04, 286.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1534)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682574 reads in 149.893 CPU sec, 4.793 real sec
[M::mem_process_seqs] Processed 682882 reads in 93.478 CPU sec, 2.955 real sec
[M::process] read 682414 sequences (100000031 bp) in 1.767 CPU sec, 1.795 real sec...
sorting block 18, #reads = 1079804 mem = 536879075 peak = 933191680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 282441, 3, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 242, 587)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1425)
[M::mem_pestat] mean and std.dev: (323.67, 217.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1844)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 321, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (337.91, 164.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 286, 556)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1190)
[M::mem_pestat] mean and std.dev: (292.94, 127.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1507)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682006 sequences (100000194 bp) in 2.659 CPU sec, 2.840 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281856, 13, 26)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 324, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (340.61, 165.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (417, 1138, 2618)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7020)
[M::mem_pestat] mean and std.dev: (1532.77, 1359.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9221)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 364, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (297.50, 108.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682678 reads in 146.987 CPU sec, 4.689 real sec
[M::mem_process_seqs] Processed 682414 reads in 91.283 CPU sec, 2.881 real sec
[M::process] read 681258 sequences (100000222 bp) in 1.851 CPU sec, 1.915 real sec...
sorting block 19, #reads = 1079644 mem = 536879837 peak = 933191680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 281765, 7, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 331, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (270.40, 121.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1075)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 327, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (343.54, 166.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (135, 250, 282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (222.33, 120.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 723)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682030 sequences (100000036 bp) in 2.349 CPU sec, 2.511 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282038, 1, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (295, 526, 1041)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2533)
[M::mem_pestat] mean and std.dev: (679.47, 408.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3279)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 331, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (346.95, 167.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 282, 1122)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2902)
[M::mem_pestat] mean and std.dev: (542.50, 491.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3792)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682006 reads in 141.347 CPU sec, 4.527 real sec
sorting block 20, #reads = 1079189 mem = 536878988 peak = 933191680
[M::mem_process_seqs] Processed 681258 reads in 87.949 CPU sec, 2.775 real sec
[M::process] read 686278 sequences (100000289 bp) in 1.897 CPU sec, 2.009 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282154, 6, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 262, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1129)
[M::mem_pestat] mean and std.dev: (258.33, 133.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1457)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 327, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (343.76, 167.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 192, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (260.14, 146.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 21, #reads = 1079766 mem = 536877206 peak = 933191680
[M::process] read 685368 sequences (100000016 bp) in 2.126 CPU sec, 2.264 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283844, 2, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 271, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (259.00, 88.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 304, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (325.46, 163.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (287, 316, 723)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1595)
[M::mem_pestat] mean and std.dev: (447.96, 262.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2031)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682030 reads in 139.509 CPU sec, 4.439 real sec
[M::process] read 684072 sequences (100000076 bp) in 1.503 CPU sec, 1.521 real sec...
[M::mem_process_seqs] Processed 686278 reads in 88.805 CPU sec, 2.808 real sec
sorting block 22, #reads = 1079498 mem = 536879171 peak = 933191680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 282939, 5, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 348, 1129)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3037)
[M::mem_pestat] mean and std.dev: (333.75, 310.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3991)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 313, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (332.41, 165.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (282, 306, 801)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1839)
[M::mem_pestat] mean and std.dev: (402.60, 279.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2358)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684224 sequences (100000286 bp) in 2.427 CPU sec, 2.617 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 282148, 6, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 190, 275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509)
[M::mem_pestat] mean and std.dev: (179.00, 65.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 626)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 318, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (336.07, 166.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 264, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608)
[M::mem_pestat] mean and std.dev: (236.60, 73.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685368 reads in 143.614 CPU sec, 4.577 real sec
[M::mem_process_seqs] Processed 684072 reads in 90.620 CPU sec, 2.861 real sec
[M::process] read 683202 sequences (100000084 bp) in 1.805 CPU sec, 1.864 real sec...
sorting block 23, #reads = 1083597 mem = 536879865 peak = 933191680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 282714, 6, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 339, 799)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2039)
[M::mem_pestat] mean and std.dev: (426.50, 334.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2659)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 318, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (335.96, 165.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 352, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (290.55, 84.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683046 sequences (100000018 bp) in 2.486 CPU sec, 2.639 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 281982, 5, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (224, 267, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (308.94, 159.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 324, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (340.13, 165.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (125, 240, 597)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1541)
[M::mem_pestat] mean and std.dev: (327.83, 257.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2013)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684224 reads in 143.814 CPU sec, 4.576 real sec
[M::process] read 682890 sequences (100000227 bp) in 1.665 CPU sec, 1.691 real sec...
[M::mem_process_seqs] Processed 683202 reads in 90.844 CPU sec, 2.868 real sec
sorting block 24, #reads = 1081340 mem = 536878623 peak = 933191680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 281867, 4, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (268, 474, 938)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2278)
[M::mem_pestat] mean and std.dev: (629.72, 429.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2948)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 325, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (340.53, 165.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 409, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1138)
[M::mem_pestat] mean and std.dev: (378.71, 169.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1445)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 25, #reads = 1080650 mem = 536880041 peak = 933191680
[M::process] read 682840 sequences (100000252 bp) in 2.063 CPU sec, 2.232 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 282194, 7, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (209, 258, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (274.29, 106.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1073)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 321, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (337.55, 163.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 481, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054)
[M::mem_pestat] mean and std.dev: (391.68, 205.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1319)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682408 sequences (100000163 bp) in 1.460 CPU sec, 1.463 real sec...
[M::mem_process_seqs] Processed 683046 reads in 143.849 CPU sec, 4.588 real sec
[M::mem_process_seqs] Processed 682890 reads in 89.590 CPU sec, 2.834 real sec
sorting block 26, #reads = 1080325 mem = 536879335 peak = 933191680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 282503, 7, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (331, 402, 904)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2050)
[M::mem_pestat] mean and std.dev: (614.46, 419.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2623)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 322, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (338.10, 164.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 447, 633)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1587)
[M::mem_pestat] mean and std.dev: (362.29, 180.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2064)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681768 sequences (100000140 bp) in 2.424 CPU sec, 2.543 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282072, 3, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (113, 267, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (300.53, 224.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 322, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (338.30, 163.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 329, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (293.56, 116.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681578 sequences (100000146 bp) in 1.490 CPU sec, 1.483 real sec...
[M::mem_process_seqs] Processed 682840 reads in 143.858 CPU sec, 4.569 real sec
[M::mem_process_seqs] Processed 682408 reads in 90.596 CPU sec, 2.866 real sec
sorting block 27, #reads = 1079621 mem = 536880020 peak = 933191680
[M::process] read 684560 sequences (100000183 bp) in 2.548 CPU sec, 2.735 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 282370, 2, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 199, 1242)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3424)
[M::mem_pestat] mean and std.dev: (542.92, 570.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4515)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 327, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (342.27, 163.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 312, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (293.90, 115.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1296)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681578 reads in 76.381 CPU sec, 2.405 real sec
[M::process] read 688658 sequences (100000186 bp) in 1.809 CPU sec, 1.872 real sec...
sorting block 28, #reads = 1078611 mem = 536882541 peak = 933191680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 281886, 6, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 306, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (285.12, 120.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 323, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (339.01, 162.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 333, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (318.23, 132.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 283043, 3, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (256, 356, 910)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2218)
[M::mem_pestat] mean and std.dev: (571.60, 557.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2872)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 316, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (335.43, 167.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 300, 938)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2306)
[M::mem_pestat] mean and std.dev: (448.12, 307.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2990)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681768 reads in 216.708 CPU sec, 6.894 real sec
[M::mem_process_seqs] Processed 684560 reads in 102.143 CPU sec, 3.238 real sec
[M::process] read 686944 sequences (100000170 bp) in 2.338 CPU sec, 2.468 real sec...
sorting block 29, #reads = 1078864 mem = 536878453 peak = 933191680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 284967, 8, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 435, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (356.36, 129.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 290, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (317.82, 164.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 234, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (256.50, 122.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685652 sequences (100000060 bp) in 2.307 CPU sec, 2.446 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 284653, 2, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (108, 236, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (220.50, 107.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 972)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 302, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (325.21, 164.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 289, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (300.25, 115.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 868)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688658 reads in 145.085 CPU sec, 4.625 real sec
[M::mem_process_seqs] Processed 686944 reads in 92.472 CPU sec, 2.918 real sec
[M::process] read 684660 sequences (100000067 bp) in 1.867 CPU sec, 1.949 real sec...
sorting block 30, #reads = 1081833 mem = 536878567 peak = 933191680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283150, 3, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 262, 550)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1260)
[M::mem_pestat] mean and std.dev: (334.12, 166.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1615)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 311, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (331.88, 167.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 280, 859)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2253)
[M::mem_pestat] mean and std.dev: (487.54, 471.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2950)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684246 sequences (100000118 bp) in 2.473 CPU sec, 2.632 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 283461, 5, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 342, 631)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1523)
[M::mem_pestat] mean and std.dev: (417.83, 282.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1969)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 318, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (337.51, 168.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 406, 531)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1099)
[M::mem_pestat] mean and std.dev: (372.50, 205.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1383)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685652 reads in 146.916 CPU sec, 4.674 real sec
[M::process] read 683624 sequences (100000087 bp) in 1.615 CPU sec, 1.639 real sec...
[M::mem_process_seqs] Processed 684660 reads in 91.433 CPU sec, 2.884 real sec
sorting block 31, #reads = 1080972 mem = 536882143 peak = 933191680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 283268, 5, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (197, 486, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1154)
[M::mem_pestat] mean and std.dev: (307.75, 158.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1473)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 322, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (340.26, 169.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (318, 378, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (28, 753)
[M::mem_pestat] mean and std.dev: (342.39, 114.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 898)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 32, #reads = 1079298 mem = 536879524 peak = 933191680
[M::process] read 683344 sequences (100000221 bp) in 2.580 CPU sec, 2.710 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 282328, 5, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 229, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (237.85, 96.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 322, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (339.76, 166.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 292, 943)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2389)
[M::mem_pestat] mean and std.dev: (407.79, 305.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3112)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683060 sequences (100000240 bp) in 1.400 CPU sec, 1.405 real sec...
[M::mem_process_seqs] Processed 684246 reads in 148.816 CPU sec, 4.726 real sec
[M::mem_process_seqs] Processed 683624 reads in 94.325 CPU sec, 2.986 real sec
sorting block 33, #reads = 1077364 mem = 536881757 peak = 933191680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282464, 12, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 534, 544)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1218)
[M::mem_pestat] mean and std.dev: (386.83, 263.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1555)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 323, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (339.81, 166.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (930, 1815, 1815)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3585)
[M::mem_pestat] mean and std.dev: (1227.20, 753.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4470)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 274, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (241.12, 105.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 683030 sequences (100000195 bp) in 2.768 CPU sec, 2.913 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 282576, 4, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 364, 1209)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3253)
[M::mem_pestat] mean and std.dev: (680.50, 778.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4275)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 323, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (340.14, 165.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 355, 609)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1427)
[M::mem_pestat] mean and std.dev: (389.83, 214.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1836)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683070 sequences (100000073 bp) in 1.480 CPU sec, 1.481 real sec...
[M::mem_process_seqs] Processed 683344 reads in 150.847 CPU sec, 4.786 real sec
[M::mem_process_seqs] Processed 683060 reads in 95.882 CPU sec, 3.029 real sec
sorting block 34, #reads = 1078480 mem = 536880055 peak = 933191680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282488, 7, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 275, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (252.46, 91.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 320, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (336.78, 163.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 524, 887)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2235)
[M::mem_pestat] mean and std.dev: (461.75, 295.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2909)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684694 sequences (100000289 bp) in 2.576 CPU sec, 2.708 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282488, 3, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (221, 320, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (327.00, 131.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 321, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (337.44, 163.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 295, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863)
[M::mem_pestat] mean and std.dev: (277.81, 125.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1089)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 690516 sequences (100000223 bp) in 1.590 CPU sec, 1.596 real sec...
[M::mem_process_seqs] Processed 683030 reads in 148.213 CPU sec, 4.700 real sec
[M::mem_process_seqs] Processed 683070 reads in 93.003 CPU sec, 2.941 real sec
sorting block 35, #reads = 1076583 mem = 536881836 peak = 933191680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 283709, 8, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 350, 1043)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2765)
[M::mem_pestat] mean and std.dev: (682.44, 718.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3626)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 314, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (334.45, 167.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 321, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (241.40, 88.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 688266 sequences (100000071 bp) in 2.546 CPU sec, 2.712 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 286286, 4, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 231, 559)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1301)
[M::mem_pestat] mean and std.dev: (387.40, 351.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1795)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 280, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (307.23, 157.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (116, 274, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860)
[M::mem_pestat] mean and std.dev: (252.07, 142.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687898 sequences (100000135 bp) in 1.460 CPU sec, 1.454 real sec...
[M::mem_process_seqs] Processed 684694 reads in 149.754 CPU sec, 4.757 real sec
sorting block 36, #reads = 1077240 mem = 536877094 peak = 933191680
[M::mem_process_seqs] Processed 690516 reads in 94.700 CPU sec, 2.998 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 285428, 5, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (209, 245, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (267.25, 85.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 293, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (316.77, 160.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 37, #reads = 1077305 mem = 536879168 peak = 999763968
[M::process] read 686242 sequences (100000243 bp) in 2.593 CPU sec, 2.720 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 285333, 8, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (230, 410, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1118)
[M::mem_pestat] mean and std.dev: (327.00, 147.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1414)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 295, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (317.81, 160.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 357, 661)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1527)
[M::mem_pestat] mean and std.dev: (384.23, 202.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1960)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685324 sequences (100000263 bp) in 1.570 CPU sec, 1.573 real sec...
[M::mem_process_seqs] Processed 688266 reads in 149.346 CPU sec, 4.738 real sec
[M::mem_process_seqs] Processed 687898 reads in 94.829 CPU sec, 3.000 real sec
sorting block 38, #reads = 1079310 mem = 536879331 peak = 999763968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 285072, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (201, 215, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (238.17, 94.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 733)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 307, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (326.96, 162.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 684292 sequences (100000074 bp) in 2.749 CPU sec, 2.924 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 284298, 2, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 305, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (320.64, 173.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 315, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (333.88, 166.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 391, 1619)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4381)
[M::mem_pestat] mean and std.dev: (1266.72, 1523.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7360)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686242 reads in 145.721 CPU sec, 4.630 real sec
[M::process] read 683710 sequences (100000257 bp) in 1.697 CPU sec, 1.720 real sec...
[M::mem_process_seqs] Processed 685324 reads in 93.426 CPU sec, 2.942 real sec
sorting block 39, #reads = 1079030 mem = 536878303 peak = 999763968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283510, 9, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 290, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (293.17, 163.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 318, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (337.19, 167.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 321, 700)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1796)
[M::mem_pestat] mean and std.dev: (396.15, 305.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2344)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683364 sequences (100000043 bp) in 2.553 CPU sec, 2.704 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283634, 10, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 280, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1057)
[M::mem_pestat] mean and std.dev: (372.45, 270.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1456)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 320, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (338.78, 167.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 877, 9063)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 26891)
[M::mem_pestat] mean and std.dev: (3151.10, 3923.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 35805)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 404, 937)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2329)
[M::mem_pestat] mean and std.dev: (506.45, 325.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3025)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 682928 sequences (100000294 bp) in 1.560 CPU sec, 1.555 real sec...
[M::mem_process_seqs] Processed 684292 reads in 149.672 CPU sec, 4.754 real sec
sorting block 40, #reads = 1077097 mem = 536878657 peak = 999763968
[M::mem_process_seqs] Processed 683710 reads in 94.595 CPU sec, 2.989 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 283110, 6, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 317, 678)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1752)
[M::mem_pestat] mean and std.dev: (392.94, 313.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2289)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 324, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (342.07, 168.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 275, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (268.17, 102.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 962)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 41, #reads = 1077121 mem = 536879460 peak = 999763968
[M::process] read 682598 sequences (100000148 bp) in 2.754 CPU sec, 2.874 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282819, 6, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 210, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (250.33, 112.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 889)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 326, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (345.39, 171.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 235, 597)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1405)
[M::mem_pestat] mean and std.dev: (362.20, 268.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1809)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683364 reads in 145.575 CPU sec, 4.620 real sec
[M::process] read 687808 sequences (100000166 bp) in 1.469 CPU sec, 1.466 real sec...
[M::mem_process_seqs] Processed 682928 reads in 92.121 CPU sec, 2.907 real sec
sorting block 42, #reads = 1077845 mem = 536879015 peak = 999763968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 282756, 5, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (223, 255, 292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (85, 430)
[M::mem_pestat] mean and std.dev: (224.00, 41.24)
[M::mem_pestat] low and high boundaries for proper pairs: (16, 499)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 332, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (352.12, 177.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 462, 560)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1190)
[M::mem_pestat] mean and std.dev: (412.73, 237.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1505)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687530 sequences (100000227 bp) in 2.454 CPU sec, 2.583 real sec...
[M::process] read 686600 sequences (100000191 bp) in 1.488 CPU sec, 1.484 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 284136, 4, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 221, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (238.21, 111.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 296, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (321.88, 165.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 281, 852)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2022)
[M::mem_pestat] mean and std.dev: (457.95, 402.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2607)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682598 reads in 150.479 CPU sec, 4.775 real sec
[M::mem_process_seqs] Processed 687808 reads in 97.544 CPU sec, 3.092 real sec
sorting block 43, #reads = 1076364 mem = 536879784 peak = 999763968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 284940, 3, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (245, 357, 1020)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2570)
[M::mem_pestat] mean and std.dev: (464.77, 351.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3345)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 298, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (319.59, 160.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (384, 416, 542)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 858)
[M::mem_pestat] mean and std.dev: (425.25, 135.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685272 sequences (100000233 bp) in 2.743 CPU sec, 2.877 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 284248, 6, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 292, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (310.59, 204.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1296)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 302, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (322.72, 161.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 408, 970)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2434)
[M::mem_pestat] mean and std.dev: (477.83, 372.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3166)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687530 reads in 143.253 CPU sec, 4.544 real sec
[M::process] read 685328 sequences (100000084 bp) in 1.649 CPU sec, 1.659 real sec...
[M::mem_process_seqs] Processed 686600 reads in 90.177 CPU sec, 2.842 real sec
sorting block 44, #reads = 1078933 mem = 536879008 peak = 999763968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283426, 4, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 223, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (269.06, 124.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1086)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 311, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (330.51, 164.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (338, 605, 765)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1619)
[M::mem_pestat] mean and std.dev: (523.93, 288.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2046)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684584 sequences (100000183 bp) in 2.349 CPU sec, 2.457 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283817, 3, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 292, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046)
[M::mem_pestat] mean and std.dev: (317.18, 162.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1337)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 313, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (330.88, 163.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 321, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (355.84, 156.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683688 sequences (100000085 bp) in 1.430 CPU sec, 1.432 real sec...
[M::mem_process_seqs] Processed 685272 reads in 146.754 CPU sec, 4.664 real sec
sorting block 45, #reads = 1080768 mem = 536879526 peak = 999763968
[M::mem_process_seqs] Processed 685328 reads in 92.273 CPU sec, 2.921 real sec
sorting block 46, #reads = 1078745 mem = 536881969 peak = 999763968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 283867, 2, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (221, 253, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866)
[M::mem_pestat] mean and std.dev: (293.67, 146.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1081)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 317, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (335.29, 165.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 226, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (270.06, 140.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683340 sequences (100000182 bp) in 2.420 CPU sec, 2.523 real sec...
[M::process] read 683390 sequences (100000161 bp) in 1.430 CPU sec, 1.428 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282708, 3, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 328, 830)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2130)
[M::mem_pestat] mean and std.dev: (479.16, 408.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2780)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 322, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (338.74, 165.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 217, 596)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1478)
[M::mem_pestat] mean and std.dev: (291.24, 243.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1919)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684584 reads in 148.561 CPU sec, 4.718 real sec
[M::mem_process_seqs] Processed 683688 reads in 95.171 CPU sec, 3.017 real sec
sorting block 47, #reads = 1078947 mem = 536877952 peak = 999763968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 283040, 2, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (245, 472, 552)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1166)
[M::mem_pestat] mean and std.dev: (376.57, 204.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1473)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 321, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (338.86, 165.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 278, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (337.44, 201.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683144 sequences (100000264 bp) in 2.378 CPU sec, 2.502 real sec...
[M::process] read 683116 sequences (100000128 bp) in 1.460 CPU sec, 1.458 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 283174, 0, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 331, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1141)
[M::mem_pestat] mean and std.dev: (335.93, 197.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1458)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 319, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (337.94, 166.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (280, 415, 774)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1762)
[M::mem_pestat] mean and std.dev: (498.32, 331.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2256)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683340 reads in 151.776 CPU sec, 4.821 real sec
[M::mem_process_seqs] Processed 683390 reads in 96.700 CPU sec, 3.068 real sec
sorting block 48, #reads = 1076643 mem = 536882296 peak = 999763968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283136, 5, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 227, 534)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1182)
[M::mem_pestat] mean and std.dev: (367.59, 269.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1506)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 322, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (339.97, 166.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 352, 613)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1313)
[M::mem_pestat] mean and std.dev: (333.08, 133.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1663)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 691090 sequences (100000028 bp) in 3.292 CPU sec, 3.462 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 283213, 8, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (233, 566, 684)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1586)
[M::mem_pestat] mean and std.dev: (487.88, 266.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2037)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 323, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (342.44, 169.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 487, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1061)
[M::mem_pestat] mean and std.dev: (399.59, 220.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1340)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683144 reads in 153.518 CPU sec, 4.880 real sec
[M::mem_process_seqs] Processed 683116 reads in 100.102 CPU sec, 3.153 real sec
sorting block 49, #reads = 1074795 mem = 536879877 peak = 999763968
[M::process] read 689652 sequences (100000245 bp) in 2.217 CPU sec, 2.329 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 285926, 6, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (212, 246, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (26, 491)
[M::mem_pestat] mean and std.dev: (243.76, 92.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 613)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 275, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (303.42, 156.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 281, 576)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1268)
[M::mem_pestat] mean and std.dev: (398.74, 305.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1621)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 690072 sequences (100000292 bp) in 2.423 CPU sec, 2.562 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 286073, 4, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 206, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (232.07, 105.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 284, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (309.21, 156.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 466, 1123)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2831)
[M::mem_pestat] mean and std.dev: (641.22, 449.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3685)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 691090 reads in 155.754 CPU sec, 4.967 real sec
sorting block 50, #reads = 1071910 mem = 536879078 peak = 999763968
[M::mem_process_seqs] Processed 689652 reads in 96.643 CPU sec, 3.044 real sec
[M::process] read 688002 sequences (100000161 bp) in 1.841 CPU sec, 1.878 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 286566, 4, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 216, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564)
[M::mem_pestat] mean and std.dev: (272.73, 133.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 281, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (307.24, 155.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (136, 179, 552)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1384)
[M::mem_pestat] mean and std.dev: (251.05, 183.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1800)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 51, #reads = 1077606 mem = 536878389 peak = 999763968
[M::process] read 686334 sequences (100000097 bp) in 2.644 CPU sec, 2.801 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 284913, 4, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 232, 831)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2125)
[M::mem_pestat] mean and std.dev: (347.47, 275.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2772)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 293, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (317.96, 161.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 304, 550)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1198)
[M::mem_pestat] mean and std.dev: (391.85, 291.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1558)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 690072 reads in 154.401 CPU sec, 4.905 real sec
[M::process] read 684784 sequences (100000042 bp) in 1.520 CPU sec, 1.516 real sec...
[M::mem_process_seqs] Processed 688002 reads in 96.833 CPU sec, 3.063 real sec
sorting block 52, #reads = 1077758 mem = 536878265 peak = 999763968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284075, 6, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (271, 420, 573)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1177)
[M::mem_pestat] mean and std.dev: (369.64, 165.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1479)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 306, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (327.05, 163.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 282, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (115, 460)
[M::mem_pestat] mean and std.dev: (262.24, 75.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 564)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684156 sequences (100000198 bp) in 2.249 CPU sec, 2.399 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 283655, 4, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (218, 582, 582)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1310)
[M::mem_pestat] mean and std.dev: (485.92, 306.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1712)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 314, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (333.11, 163.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 308, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (324.00, 144.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683272 sequences (100000138 bp) in 1.900 CPU sec, 1.897 real sec...
[M::mem_process_seqs] Processed 686334 reads in 153.048 CPU sec, 4.855 real sec
[M::mem_process_seqs] Processed 684784 reads in 97.299 CPU sec, 3.072 real sec
sorting block 53, #reads = 1076969 mem = 536878675 peak = 999763968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 283211, 6, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (260, 402, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (395.35, 166.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 317, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (336.02, 166.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 339, 1032)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2646)
[M::mem_pestat] mean and std.dev: (521.81, 455.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3453)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682684 sequences (100000277 bp) in 2.405 CPU sec, 2.560 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 283319, 4, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 292, 2297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6513)
[M::mem_pestat] mean and std.dev: (796.38, 991.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8621)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 321, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (338.39, 165.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (325, 484, 2326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6328)
[M::mem_pestat] mean and std.dev: (1068.26, 1150.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8329)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682242 sequences (100000108 bp) in 1.390 CPU sec, 1.388 real sec...
[M::mem_process_seqs] Processed 684156 reads in 148.216 CPU sec, 4.712 real sec
sorting block 54, #reads = 1075056 mem = 536880741 peak = 999763968
[M::mem_process_seqs] Processed 683272 reads in 93.282 CPU sec, 2.953 real sec
[M::process] read 216316 sequences (31685676 bp) in 1.277 CPU sec, 1.330 real sec...
sorting block 55, #reads = 1077218 mem = 536879755 peak = 1006178304
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282371, 18, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 174, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1155)
[M::mem_pestat] mean and std.dev: (281.40, 199.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1494)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 324, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (341.52, 166.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (349, 499, 621)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1165)
[M::mem_pestat] mean and std.dev: (446.89, 165.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1437)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 776, 1331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3453)
[M::mem_pestat] mean and std.dev: (890.75, 782.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4514)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282538, 5, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 290, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (270.36, 143.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 329, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (346.05, 169.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 682684 reads in 143.074 CPU sec, 4.619 real sec
[M::mem_process_seqs] Processed 682242 reads in 89.387 CPU sec, 2.893 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 89518, 0, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (234, 419, 645)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1467)
[M::mem_pestat] mean and std.dev: (432.00, 211.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1878)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 56, #reads = 1077047 mem = 536877962 peak = 1006178304
[M::mem_pestat] (25, 50, 75) percentile: (202, 323, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (341.78, 167.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 216316 reads in 26.779 CPU sec, 0.902 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 61792826
overall: 69866012672 mem 8508.422 user 383.512 sys 418.252 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_3U6-T1-TDNA-1_A23MHWWLT4_1.L002\tPU:L002\tSM:1136_3U6-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_3U6-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0001.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0001.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 418.288 sec; CPU: 8891.937 sec
sorting block 57, #reads = 613541 mem = 306324225 peak = 1058267136
uniq header count 1
merging 58 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3599048812
output reads: 62123012
bam_mem_sort: 58 calls 28.818 user 0.293 sys 29.248 real
bam_write: 58 calls 14.756 user 0.603 sys 15.302 real
execute: 1 calls 13.494 user 0.743 sys 439.021 real
merge_files: 1 calls 13.479 user 0.713 sys 19.840 real
parse_chunk: 922 calls 37.975 user 8.716 sys 47.217 real
read_chunk: 2487 calls 19.716 user 19.960 sys 238.046 real
sort_block: 1 calls 15.489 user 19.340 sys 230.500 real
write_chunk: 1563 calls 13.167 user 0.543 sys 19.060 real
overall: 2654203904 mem 774.465 user 38.275 sys 439.041 real
ls: cannot access '*.cram': No such file or directory