This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_3LU-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2004.566 CPU sec, 83.068 real sec [M::process] read 679492 sequences (100000290 bp) in 1.318 CPU sec, 1.322 real sec... [M::process] read 679138 sequences (100000203 bp) in 2.578 CPU sec, 2.595 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 283129, 2, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 297, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (317.00, 145.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (237, 369, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041) [M::mem_pestat] mean and std.dev: (380.35, 178.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1309) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 260, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003) [M::mem_pestat] mean and std.dev: (283.28, 131.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1271) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678576 sequences (100000170 bp) in 2.378 CPU sec, 2.393 real sec... [M::process] read 678008 sequences (100000291 bp) in 2.020 CPU sec, 2.035 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 283189, 1, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 250, 1378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3774) [M::mem_pestat] mean and std.dev: (633.81, 701.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4972) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (240, 374, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050) [M::mem_pestat] mean and std.dev: (383.71, 178.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1320) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 354, 585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1445) [M::mem_pestat] mean and std.dev: (402.48, 211.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1875) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 679492 reads in 269.983 CPU sec, 8.652 real sec [M::mem_process_seqs] Processed 679138 reads in 148.018 CPU sec, 4.779 real sec sorting block 0, #reads = 1080550 mem = 536878481 peak = 588017664 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282750, 6, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 239, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (37, 502) [M::mem_pestat] mean and std.dev: (235.19, 80.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 379, 515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1059) [M::mem_pestat] mean and std.dev: (388.27, 180.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1331) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (265, 400, 547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1111) [M::mem_pestat] mean and std.dev: (328.86, 137.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1393) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677602 sequences (100000206 bp) in 2.772 CPU sec, 3.283 real sec... [M::process] read 677434 sequences (100000163 bp) in 1.864 CPU sec, 1.882 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 282144, 4, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 310, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (295.83, 156.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 384, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1070) [M::mem_pestat] mean and std.dev: (393.69, 182.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1344) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 295, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (296.21, 173.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 678576 reads in 210.676 CPU sec, 6.835 real sec [M::mem_process_seqs] Processed 678008 reads in 137.908 CPU sec, 4.583 real sec sorting block 1, #reads = 1078827 mem = 536879968 peak = 719089664 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281538, 5, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (247, 264, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (308.58, 106.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (251, 388, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1073) [M::mem_pestat] mean and std.dev: (396.59, 182.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1347) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 275, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1107) [M::mem_pestat] mean and std.dev: (325.36, 209.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1415) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677268 sequences (100000225 bp) in 2.730 CPU sec, 3.406 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 281928, 6, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 367, 620) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1514) [M::mem_pestat] mean and std.dev: (375.12, 295.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1961) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (253, 391, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1072) [M::mem_pestat] mean and std.dev: (398.12, 182.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1345) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 364, 1536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4152) [M::mem_pestat] mean and std.dev: (517.34, 465.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5460) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677602 reads in 218.631 CPU sec, 7.237 real sec [M::process] read 677350 sequences (100000007 bp) in 2.282 CPU sec, 2.313 real sec... [M::mem_process_seqs] Processed 677434 reads in 147.474 CPU sec, 4.946 real sec sorting block 2, #reads = 1079095 mem = 536880625 peak = 829759488 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 281439, 6, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 393, 563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1367) [M::mem_pestat] mean and std.dev: (328.89, 179.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1769) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (254, 392, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076) [M::mem_pestat] mean and std.dev: (399.11, 182.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1350) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (259, 383, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (338.61, 128.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676866 sequences (100000193 bp) in 3.469 CPU sec, 4.029 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 282503, 0, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 282, 1442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4002) [M::mem_pestat] mean and std.dev: (319.03, 250.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5282) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (255, 393, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1077) [M::mem_pestat] mean and std.dev: (399.93, 182.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1351) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (272, 347, 575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1181) [M::mem_pestat] mean and std.dev: (410.48, 204.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1484) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677268 reads in 223.209 CPU sec, 7.379 real sec [M::mem_process_seqs] Processed 677350 reads in 147.468 CPU sec, 4.853 real sec [M::process] read 676574 sequences (100000035 bp) in 2.677 CPU sec, 2.886 real sec... sorting block 3, #reads = 1079703 mem = 536880201 peak = 829759488 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 281313, 5, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 316, 611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1371) [M::mem_pestat] mean and std.dev: (334.15, 186.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1751) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (256, 394, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075) [M::mem_pestat] mean and std.dev: (400.39, 181.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1348) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 337, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969) [M::mem_pestat] mean and std.dev: (322.38, 162.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1227) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676384 sequences (100000038 bp) in 3.266 CPU sec, 3.696 real sec... sorting block 4, #reads = 1080567 mem = 536880911 peak = 849596416 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 281460, 0, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (256, 290, 478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (312.21, 161.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (259, 397, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1081) [M::mem_pestat] mean and std.dev: (403.79, 182.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (273, 415, 704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1566) [M::mem_pestat] mean and std.dev: (396.15, 174.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1997) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676866 reads in 225.150 CPU sec, 7.661 real sec [M::mem_process_seqs] Processed 676574 reads in 150.407 CPU sec, 5.122 real sec [M::process] read 676274 sequences (100000055 bp) in 2.692 CPU sec, 3.228 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 281402, 11, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 223, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (230.73, 96.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (262, 402, 539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093) [M::mem_pestat] mean and std.dev: (407.89, 184.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1370) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (964, 964, 6468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17476) [M::mem_pestat] mean and std.dev: (2889.91, 2706.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22980) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 381, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833) [M::mem_pestat] mean and std.dev: (325.88, 110.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 5, #reads = 1079527 mem = 536880040 peak = 849596416 [M::process] read 677470 sequences (100000137 bp) in 3.500 CPU sec, 4.092 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 281324, 1, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (252, 274, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (170, 375) [M::mem_pestat] mean and std.dev: (265.56, 37.76) [M::mem_pestat] low and high boundaries for proper pairs: (115, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (263, 403, 541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1097) [M::mem_pestat] mean and std.dev: (408.99, 184.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1375) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 291, 561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1303) [M::mem_pestat] mean and std.dev: (326.78, 189.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1674) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676384 reads in 229.301 CPU sec, 7.889 real sec [M::mem_process_seqs] Processed 676274 reads in 153.045 CPU sec, 5.328 real sec [M::process] read 677402 sequences (100000289 bp) in 3.845 CPU sec, 4.649 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 281950, 1, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (244, 434, 548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1156) [M::mem_pestat] mean and std.dev: (384.11, 141.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (254, 394, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1082) [M::mem_pestat] mean and std.dev: (400.77, 183.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1358) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 456, 864) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2206) [M::mem_pestat] mean and std.dev: (396.38, 264.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2877) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677470 reads in 131.620 CPU sec, 4.634 real sec sorting block 6, #reads = 1079249 mem = 536879454 peak = 849596416 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 281615, 2, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 312, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1179) [M::mem_pestat] mean and std.dev: (310.10, 169.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (252, 392, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1086) [M::mem_pestat] mean and std.dev: (399.75, 183.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1364) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (266, 342, 588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1232) [M::mem_pestat] mean and std.dev: (380.46, 200.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1554) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676966 sequences (100000086 bp) in 3.550 CPU sec, 4.195 real sec... [M::mem_process_seqs] Processed 677402 reads in 132.041 CPU sec, 4.634 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 281147, 6, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (222, 364, 687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1617) [M::mem_pestat] mean and std.dev: (389.91, 195.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2082) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (253, 393, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1087) [M::mem_pestat] mean and std.dev: (400.40, 183.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1365) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (244, 310, 551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1165) [M::mem_pestat] mean and std.dev: (353.96, 205.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1472) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677066 sequences (100000199 bp) in 3.444 CPU sec, 4.271 real sec... sorting block 7, #reads = 1079842 mem = 536879055 peak = 849596416 [M::mem_process_seqs] Processed 676966 reads in 132.665 CPU sec, 4.748 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 281640, 4, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 246, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1105) [M::mem_pestat] mean and std.dev: (288.48, 155.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (255, 394, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1083) [M::mem_pestat] mean and std.dev: (400.87, 183.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1359) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 296, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076) [M::mem_pestat] mean and std.dev: (322.48, 137.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1370) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676908 sequences (100000274 bp) in 3.367 CPU sec, 4.428 real sec... [M::mem_process_seqs] Processed 677066 reads in 132.060 CPU sec, 4.805 real sec sorting block 8, #reads = 1080253 mem = 536879278 peak = 849596416 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281548, 2, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (284, 291, 1114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2774) [M::mem_pestat] mean and std.dev: (417.23, 357.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (256, 394, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1084) [M::mem_pestat] mean and std.dev: (401.19, 183.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1360) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 229, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (220.52, 100.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676912 sequences (100000274 bp) in 3.496 CPU sec, 4.041 real sec... [M::mem_process_seqs] Processed 676908 reads in 135.156 CPU sec, 4.756 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 280996, 11, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 220, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (212.21, 89.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (256, 393, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075) [M::mem_pestat] mean and std.dev: (400.59, 182.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1348) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1901, 1901, 1901) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1901, 1901) [M::mem_pestat] mean and std.dev: (1901.00, 0.00) [M::mem_pestat] low and high boundaries for proper pairs: (1901, 1901) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 233, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (265.50, 130.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 676904 sequences (100000274 bp) in 3.431 CPU sec, 4.122 real sec... [M::mem_process_seqs] Processed 676912 reads in 136.716 CPU sec, 4.780 real sec sorting block 9, #reads = 1080177 mem = 536876862 peak = 849596416 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 280605, 3, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 230, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (233.47, 82.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (255, 392, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1077) [M::mem_pestat] mean and std.dev: (399.59, 182.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1351) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 294, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (331.77, 132.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1001) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676808 sequences (100000062 bp) in 3.405 CPU sec, 3.982 real sec... [M::mem_process_seqs] Processed 676904 reads in 135.024 CPU sec, 4.722 real sec sorting block 10, #reads = 1079878 mem = 536877518 peak = 849596416 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 280385, 4, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 213, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004) [M::mem_pestat] mean and std.dev: (286.79, 151.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1278) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (255, 391, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1077) [M::mem_pestat] mean and std.dev: (399.73, 182.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1351) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 488, 640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1504) [M::mem_pestat] mean and std.dev: (468.53, 289.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1936) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676840 sequences (100000112 bp) in 3.801 CPU sec, 4.311 real sec... [M::mem_process_seqs] Processed 676808 reads in 132.460 CPU sec, 4.544 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 281260, 4, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 286, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (257.11, 110.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (254, 387, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1061) [M::mem_pestat] mean and std.dev: (395.54, 179.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1330) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (420, 485, 6727) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 19341) [M::mem_pestat] mean and std.dev: (2507.28, 2892.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 25648) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676840 reads in 131.197 CPU sec, 4.497 real sec [M::process] read 676892 sequences (100000184 bp) in 3.977 CPU sec, 4.585 real sec... sorting block 11, #reads = 1079068 mem = 536879524 peak = 870223872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 280940, 0, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 269, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (274.87, 150.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (252, 384, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053) [M::mem_pestat] mean and std.dev: (393.31, 178.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1320) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 303, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (277.08, 104.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677338 sequences (100000274 bp) in 3.668 CPU sec, 4.120 real sec... [M::mem_process_seqs] Processed 676892 reads in 131.428 CPU sec, 4.432 real sec sorting block 12, #reads = 1078949 mem = 536881073 peak = 870223872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282015, 1, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 317, 680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1642) [M::mem_pestat] mean and std.dev: (277.81, 146.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2123) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 378, 512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040) [M::mem_pestat] mean and std.dev: (387.41, 176.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1304) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (293, 393, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (379.03, 132.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677338 reads in 128.866 CPU sec, 4.323 real sec [M::process] read 679610 sequences (100000246 bp) in 4.024 CPU sec, 4.431 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 283152, 4, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 234, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (241.55, 125.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (234, 359, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990) [M::mem_pestat] mean and std.dev: (368.44, 168.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1242) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (251, 325, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797) [M::mem_pestat] mean and std.dev: (314.02, 133.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 679610 reads in 130.641 CPU sec, 4.358 real sec [M::process] read 677222 sequences (100000086 bp) in 4.094 CPU sec, 4.577 real sec... sorting block 13, #reads = 1079011 mem = 536877634 peak = 870223872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 280830, 0, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 336, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804) [M::mem_pestat] mean and std.dev: (328.00, 152.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1000) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 378, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035) [M::mem_pestat] mean and std.dev: (387.89, 176.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1297) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 287, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (252.26, 93.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677222 reads in 120.441 CPU sec, 4.031 real sec [M::process] read 677220 sequences (100000150 bp) in 3.796 CPU sec, 4.192 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 280220, 1, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 275, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (279.15, 146.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 378, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035) [M::mem_pestat] mean and std.dev: (388.19, 176.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1297) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 332, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978) [M::mem_pestat] mean and std.dev: (310.58, 140.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1233) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 14, #reads = 1077549 mem = 536878781 peak = 870223872 [M::mem_process_seqs] Processed 677220 reads in 124.571 CPU sec, 4.149 real sec [M::process] read 677160 sequences (100000082 bp) in 3.883 CPU sec, 4.270 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 280462, 2, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 316, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964) [M::mem_pestat] mean and std.dev: (280.68, 118.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1214) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 378, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044) [M::mem_pestat] mean and std.dev: (389.70, 178.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1309) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (274, 424, 605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1267) [M::mem_pestat] mean and std.dev: (440.00, 181.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1598) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676836 sequences (100000231 bp) in 3.260 CPU sec, 3.614 real sec... [M::mem_process_seqs] Processed 677160 reads in 124.927 CPU sec, 4.191 real sec sorting block 15, #reads = 1076988 mem = 536881981 peak = 870223872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 280869, 3, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 252, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (276.41, 115.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (250, 380, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051) [M::mem_pestat] mean and std.dev: (391.39, 179.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1318) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 292, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (296.24, 131.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676836 reads in 117.852 CPU sec, 3.930 real sec [M::process] read 677128 sequences (100000252 bp) in 4.228 CPU sec, 4.784 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 281067, 0, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (243, 314, 3225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9189) [M::mem_pestat] mean and std.dev: (1690.91, 2072.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12171) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 377, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043) [M::mem_pestat] mean and std.dev: (389.01, 178.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1308) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 252, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1089) [M::mem_pestat] mean and std.dev: (289.25, 179.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1398) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 16, #reads = 1079637 mem = 536878261 peak = 870223872 [M::mem_process_seqs] Processed 677128 reads in 116.272 CPU sec, 3.863 real sec [M::process] read 676878 sequences (100000164 bp) in 3.901 CPU sec, 4.423 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 281408, 1, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 259, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (313.58, 168.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (250, 379, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042) [M::mem_pestat] mean and std.dev: (389.96, 178.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1306) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 314, 571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1303) [M::mem_pestat] mean and std.dev: (336.05, 171.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1669) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676878 reads in 118.247 CPU sec, 3.920 real sec [M::process] read 676606 sequences (100000083 bp) in 4.140 CPU sec, 4.616 real sec... sorting block 17, #reads = 1079230 mem = 536878179 peak = 870223872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 280997, 1, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 279, 900) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2408) [M::mem_pestat] mean and std.dev: (331.55, 270.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3162) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (252, 382, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053) [M::mem_pestat] mean and std.dev: (393.47, 179.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1320) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (247, 349, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (278.62, 104.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676606 reads in 113.076 CPU sec, 3.715 real sec [M::process] read 676648 sequences (100000009 bp) in 3.326 CPU sec, 3.939 real sec... sorting block 18, #reads = 1078945 mem = 536880517 peak = 870223872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 281052, 1, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 281, 555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1235) [M::mem_pestat] mean and std.dev: (285.12, 145.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (252, 382, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047) [M::mem_pestat] mean and std.dev: (392.66, 179.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1312) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (291, 563, 1921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5181) [M::mem_pestat] mean and std.dev: (1052.41, 1339.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6811) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676648 reads in 108.543 CPU sec, 3.564 real sec [M::process] read 676504 sequences (100000163 bp) in 4.296 CPU sec, 4.768 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 281043, 1, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 261, 539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1289) [M::mem_pestat] mean and std.dev: (342.14, 178.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (252, 382, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050) [M::mem_pestat] mean and std.dev: (393.37, 179.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1316) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (261, 361, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (341.88, 141.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 19, #reads = 1078820 mem = 536877734 peak = 870223872 [M::mem_process_seqs] Processed 676504 reads in 104.904 CPU sec, 3.416 real sec [M::process] read 676488 sequences (100000288 bp) in 4.273 CPU sec, 4.841 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 281266, 1, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 264, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (312.15, 150.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (253, 382, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048) [M::mem_pestat] mean and std.dev: (393.85, 179.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1313) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 283, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (247.59, 101.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676488 reads in 98.974 CPU sec, 3.196 real sec [M::process] read 676218 sequences (100000185 bp) in 3.084 CPU sec, 3.396 real sec... sorting block 20, #reads = 1079280 mem = 536880459 peak = 870223872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 281684, 5, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 394, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (369.62, 157.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (256, 386, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1057) [M::mem_pestat] mean and std.dev: (397.07, 180.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1324) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 273, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (283.66, 148.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1143) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676218 reads in 95.395 CPU sec, 3.085 real sec [M::process] read 676984 sequences (100000128 bp) in 3.796 CPU sec, 4.178 real sec... sorting block 21, #reads = 1079692 mem = 536882018 peak = 870223872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282276, 1, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 402, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969) [M::mem_pestat] mean and std.dev: (328.71, 146.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1222) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (250, 380, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048) [M::mem_pestat] mean and std.dev: (391.01, 179.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1314) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 373, 535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1187) [M::mem_pestat] mean and std.dev: (352.11, 187.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1513) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678518 sequences (100000079 bp) in 2.723 CPU sec, 2.967 real sec... [M::mem_process_seqs] Processed 676984 reads in 97.990 CPU sec, 3.148 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282507, 1, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 197, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (213.89, 98.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (242, 377, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055) [M::mem_pestat] mean and std.dev: (387.17, 180.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1326) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 310, 862) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2282) [M::mem_pestat] mean and std.dev: (405.80, 318.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2992) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 678518 reads in 99.162 CPU sec, 3.189 real sec [M::process] read 678242 sequences (100000056 bp) in 3.367 CPU sec, 3.714 real sec... sorting block 22, #reads = 1079996 mem = 536878046 peak = 870223872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281532, 0, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 387, 547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1235) [M::mem_pestat] mean and std.dev: (428.26, 255.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 380, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056) [M::mem_pestat] mean and std.dev: (390.30, 181.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1326) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 252, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (228.69, 94.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 678242 reads in 98.446 CPU sec, 3.183 real sec [M::process] read 677718 sequences (100000245 bp) in 3.103 CPU sec, 3.351 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 282394, 1, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 323, 735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1825) [M::mem_pestat] mean and std.dev: (406.32, 300.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2370) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 385, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068) [M::mem_pestat] mean and std.dev: (394.36, 182.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (271, 365, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012) [M::mem_pestat] mean and std.dev: (395.44, 179.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1259) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677258 sequences (100000191 bp) in 2.747 CPU sec, 3.011 real sec... [M::mem_process_seqs] Processed 677718 reads in 97.997 CPU sec, 3.156 real sec sorting block 23, #reads = 1080591 mem = 536880720 peak = 892809216 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 281720, 1, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 288, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (244.78, 101.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (253, 390, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1078) [M::mem_pestat] mean and std.dev: (399.29, 184.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1353) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 292, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (287.84, 102.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 993) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677258 reads in 103.241 CPU sec, 3.362 real sec [M::process] read 677108 sequences (100000042 bp) in 3.662 CPU sec, 3.991 real sec... sorting block 24, #reads = 1081606 mem = 536878479 peak = 892809216 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 281793, 4, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 233, 571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1383) [M::mem_pestat] mean and std.dev: (298.05, 180.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (255, 395, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1092) [M::mem_pestat] mean and std.dev: (403.49, 186.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1371) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 263, 783) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1989) [M::mem_pestat] mean and std.dev: (254.92, 165.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2592) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677108 reads in 109.759 CPU sec, 3.559 real sec [M::process] read 677060 sequences (100000109 bp) in 3.276 CPU sec, 3.681 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282001, 9, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 272, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (256.82, 105.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (256, 398, 536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1096) [M::mem_pestat] mean and std.dev: (404.98, 186.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1376) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (248, 311, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980) [M::mem_pestat] mean and std.dev: (308.40, 119.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1224) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676674 sequences (100000196 bp) in 3.089 CPU sec, 3.443 real sec... [M::mem_process_seqs] Processed 677060 reads in 108.450 CPU sec, 3.509 real sec sorting block 25, #reads = 1080347 mem = 536878674 peak = 969011200 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 281341, 1, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 167, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (212.90, 123.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (260, 402, 541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1103) [M::mem_pestat] mean and std.dev: (409.07, 187.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1384) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 269, 578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1362) [M::mem_pestat] mean and std.dev: (302.47, 146.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1754) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676674 reads in 109.987 CPU sec, 3.581 real sec [M::process] read 676606 sequences (100000035 bp) in 3.961 CPU sec, 4.435 real sec... sorting block 26, #reads = 1080266 mem = 536880130 peak = 969011200 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 281114, 3, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 223, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (266.76, 133.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (259, 400, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093) [M::mem_pestat] mean and std.dev: (406.81, 186.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1371) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 385, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (317.94, 165.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1136) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676340 sequences (100000122 bp) in 2.945 CPU sec, 3.277 real sec... [M::mem_process_seqs] Processed 676606 reads in 109.503 CPU sec, 3.533 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 281513, 1, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 313, 583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1299) [M::mem_pestat] mean and std.dev: (372.73, 198.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (261, 402, 540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1098) [M::mem_pestat] mean and std.dev: (408.61, 186.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1377) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (265, 333, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994) [M::mem_pestat] mean and std.dev: (376.46, 144.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1237) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676340 reads in 108.348 CPU sec, 3.504 real sec [M::process] read 676212 sequences (100000044 bp) in 3.722 CPU sec, 4.028 real sec... sorting block 27, #reads = 1080770 mem = 536879174 peak = 969011200 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 280987, 5, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (242, 339, 1031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2609) [M::mem_pestat] mean and std.dev: (455.06, 449.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3398) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (264, 405, 540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1092) [M::mem_pestat] mean and std.dev: (410.46, 186.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1368) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (343, 510, 550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964) [M::mem_pestat] mean and std.dev: (444.78, 134.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1171) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676212 reads in 109.211 CPU sec, 3.531 real sec [M::process] read 675936 sequences (100000130 bp) in 4.176 CPU sec, 4.579 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 281035, 12, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 328, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (268.76, 89.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (265, 408, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1105) [M::mem_pestat] mean and std.dev: (413.83, 188.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1385) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (629, 662, 1079) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1979) [M::mem_pestat] mean and std.dev: (681.45, 246.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2429) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 339, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (337.37, 125.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 675936 reads in 108.914 CPU sec, 3.522 real sec sorting block 28, #reads = 1079489 mem = 536878524 peak = 969011200 [M::process] read 676044 sequences (100000207 bp) in 4.338 CPU sec, 4.757 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 281709, 2, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 344, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (309.89, 129.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (267, 408, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1101) [M::mem_pestat] mean and std.dev: (413.60, 187.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 276, 580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1322) [M::mem_pestat] mean and std.dev: (334.03, 182.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1693) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676044 reads in 109.087 CPU sec, 3.535 real sec [M::process] read 676190 sequences (100000174 bp) in 3.750 CPU sec, 4.287 real sec... sorting block 29, #reads = 1079273 mem = 536879806 peak = 969011200 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 281302, 1, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (253, 357, 556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1162) [M::mem_pestat] mean and std.dev: (356.58, 165.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (264, 405, 544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1104) [M::mem_pestat] mean and std.dev: (411.58, 187.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1384) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (289, 303, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (7, 712) [M::mem_pestat] mean and std.dev: (309.29, 104.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676190 reads in 110.082 CPU sec, 3.566 real sec [M::process] read 677022 sequences (100000135 bp) in 4.022 CPU sec, 4.425 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 281903, 1, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 350, 564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1242) [M::mem_pestat] mean and std.dev: (349.48, 191.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (255, 396, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1101) [M::mem_pestat] mean and std.dev: (404.44, 187.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1383) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 363, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (329.05, 142.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677022 reads in 109.972 CPU sec, 3.548 real sec sorting block 30, #reads = 1080675 mem = 536879573 peak = 969011200 [M::process] read 676888 sequences (100000015 bp) in 3.819 CPU sec, 4.269 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281234, 2, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 223, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (220.89, 70.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (256, 396, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1090) [M::mem_pestat] mean and std.dev: (403.77, 185.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1368) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (257, 413, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (375.23, 146.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677054 sequences (100000136 bp) in 3.174 CPU sec, 3.502 real sec... [M::mem_process_seqs] Processed 676888 reads in 111.416 CPU sec, 3.594 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 281254, 2, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 311, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (260.51, 121.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1072) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (256, 396, 535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093) [M::mem_pestat] mean and std.dev: (403.52, 185.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1372) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (265, 387, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943) [M::mem_pestat] mean and std.dev: (343.57, 131.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1169) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 31, #reads = 1080326 mem = 536878652 peak = 969011200 [M::mem_process_seqs] Processed 677054 reads in 110.749 CPU sec, 3.572 real sec [M::process] read 676622 sequences (100000120 bp) in 3.474 CPU sec, 3.797 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 281364, 5, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 318, 567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1339) [M::mem_pestat] mean and std.dev: (328.15, 160.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1725) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (257, 396, 535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1091) [M::mem_pestat] mean and std.dev: (404.08, 185.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1369) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 256, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (277.76, 149.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676622 reads in 113.484 CPU sec, 3.677 real sec [M::process] read 676700 sequences (100000114 bp) in 3.632 CPU sec, 3.980 real sec... sorting block 32, #reads = 1079740 mem = 536880144 peak = 969011200 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 281459, 6, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (287, 359, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794) [M::mem_pestat] mean and std.dev: (354.00, 115.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (255, 393, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1086) [M::mem_pestat] mean and std.dev: (402.43, 185.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1363) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 276, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (255.07, 92.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676904 sequences (100000079 bp) in 3.165 CPU sec, 3.530 real sec... [M::mem_process_seqs] Processed 676700 reads in 109.346 CPU sec, 3.538 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 281480, 7, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 347, 1035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2633) [M::mem_pestat] mean and std.dev: (338.37, 222.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (254, 389, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076) [M::mem_pestat] mean and std.dev: (399.28, 183.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1350) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 281, 540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1168) [M::mem_pestat] mean and std.dev: (371.23, 199.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1482) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 33, #reads = 1079891 mem = 536878011 peak = 969011200 [M::process] read 676858 sequences (100000116 bp) in 3.236 CPU sec, 3.467 real sec... [M::mem_process_seqs] Processed 676904 reads in 110.310 CPU sec, 3.563 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 281825, 11, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (227, 334, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995) [M::mem_pestat] mean and std.dev: (320.86, 137.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1251) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (255, 392, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1080) [M::mem_pestat] mean and std.dev: (401.28, 184.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1355) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (2055, 2055, 2055) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (2055, 2055) [M::mem_pestat] mean and std.dev: (2055.00, 0.00) [M::mem_pestat] low and high boundaries for proper pairs: (2055, 2055) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 289, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (285.08, 91.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676858 reads in 111.442 CPU sec, 3.606 real sec [M::process] read 676976 sequences (100000128 bp) in 3.784 CPU sec, 4.108 real sec... sorting block 34, #reads = 1080332 mem = 536881849 peak = 969011200 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 281242, 0, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 278, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (289.89, 167.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (252, 387, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1071) [M::mem_pestat] mean and std.dev: (396.69, 182.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1344) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 301, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1067) [M::mem_pestat] mean and std.dev: (350.21, 192.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1358) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676976 reads in 113.051 CPU sec, 3.664 real sec [M::process] read 677252 sequences (100000068 bp) in 4.692 CPU sec, 5.195 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 281731, 3, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (234.74, 121.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 384, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1070) [M::mem_pestat] mean and std.dev: (394.09, 182.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1344) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 337, 536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1176) [M::mem_pestat] mean and std.dev: (343.11, 169.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1496) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677252 reads in 114.911 CPU sec, 3.742 real sec sorting block 35, #reads = 1079394 mem = 536877757 peak = 969011200 [M::process] read 677374 sequences (100000222 bp) in 4.414 CPU sec, 4.915 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 281693, 1, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 285, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041) [M::mem_pestat] mean and std.dev: (281.53, 128.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1321) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 383, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1070) [M::mem_pestat] mean and std.dev: (394.10, 182.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1344) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 331, 616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1426) [M::mem_pestat] mean and std.dev: (353.28, 185.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1831) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677374 reads in 111.238 CPU sec, 3.586 real sec [M::process] read 677328 sequences (100000209 bp) in 4.010 CPU sec, 4.381 real sec... sorting block 36, #reads = 1079156 mem = 536879622 peak = 969011200 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 281964, 1, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 257, 1023) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2725) [M::mem_pestat] mean and std.dev: (369.42, 311.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 381, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1063) [M::mem_pestat] mean and std.dev: (392.26, 181.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1335) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 444, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033) [M::mem_pestat] mean and std.dev: (382.60, 158.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1305) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677328 reads in 111.206 CPU sec, 3.588 real sec [M::process] read 679288 sequences (100000168 bp) in 4.228 CPU sec, 4.682 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282860, 6, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 265, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (239.03, 81.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (237, 367, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026) [M::mem_pestat] mean and std.dev: (377.29, 175.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1289) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 241, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (255.14, 113.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677216 sequences (100000234 bp) in 3.355 CPU sec, 3.591 real sec... [M::mem_process_seqs] Processed 679288 reads in 119.193 CPU sec, 3.825 real sec sorting block 37, #reads = 1077727 mem = 536879425 peak = 969011200 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 281807, 0, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 228, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978) [M::mem_pestat] mean and std.dev: (240.48, 143.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1233) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 382, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1059) [M::mem_pestat] mean and std.dev: (392.66, 181.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 329, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (292.15, 102.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677216 reads in 111.117 CPU sec, 3.581 real sec [M::process] read 676994 sequences (100000000 bp) in 3.840 CPU sec, 4.213 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 281371, 6, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 304, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1229) [M::mem_pestat] mean and std.dev: (326.48, 155.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (250, 384, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1069) [M::mem_pestat] mean and std.dev: (395.44, 183.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1342) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 331, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976) [M::mem_pestat] mean and std.dev: (313.53, 126.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1229) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 38, #reads = 1078517 mem = 536880040 peak = 969011200 [M::mem_process_seqs] Processed 676994 reads in 110.008 CPU sec, 3.552 real sec [M::process] read 677032 sequences (100000259 bp) in 3.839 CPU sec, 4.241 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 281884, 4, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 286, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (263.84, 125.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (252, 385, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068) [M::mem_pestat] mean and std.dev: (396.04, 182.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1340) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 456, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1168) [M::mem_pestat] mean and std.dev: (385.94, 201.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1493) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677032 reads in 105.198 CPU sec, 3.419 real sec [M::process] read 677250 sequences (100000008 bp) in 3.946 CPU sec, 4.385 real sec... sorting block 39, #reads = 1079466 mem = 536879239 peak = 969011200 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282220, 3, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 246, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1129) [M::mem_pestat] mean and std.dev: (297.81, 171.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 380, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055) [M::mem_pestat] mean and std.dev: (392.02, 181.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1324) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 280, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (312.58, 142.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676542 sequences (100000191 bp) in 3.405 CPU sec, 3.821 real sec... [M::mem_process_seqs] Processed 677250 reads in 119.334 CPU sec, 3.900 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 281609, 0, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 375, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1178) [M::mem_pestat] mean and std.dev: (335.61, 163.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (252, 385, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1059) [M::mem_pestat] mean and std.dev: (395.49, 181.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1328) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 323, 661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1539) [M::mem_pestat] mean and std.dev: (363.88, 259.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1978) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676542 reads in 117.268 CPU sec, 3.810 real sec [M::process] read 676696 sequences (100000192 bp) in 4.185 CPU sec, 4.704 real sec... sorting block 40, #reads = 1079857 mem = 536886252 peak = 969011200 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 281032, 3, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 270, 778) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1942) [M::mem_pestat] mean and std.dev: (463.86, 389.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (252, 382, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050) [M::mem_pestat] mean and std.dev: (393.67, 180.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1316) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 329, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (312.73, 121.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676696 reads in 119.350 CPU sec, 3.928 real sec [M::process] read 676740 sequences (100000205 bp) in 3.506 CPU sec, 4.024 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282085, 6, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 243, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (258.37, 121.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (253, 383, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048) [M::mem_pestat] mean and std.dev: (394.07, 180.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1313) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (254, 444, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052) [M::mem_pestat] mean and std.dev: (376.11, 146.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1318) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676740 reads in 113.496 CPU sec, 3.736 real sec sorting block 41, #reads = 1078667 mem = 536883494 peak = 987090944 [M::process] read 676552 sequences (100000048 bp) in 3.936 CPU sec, 4.341 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 281969, 4, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (310, 355, 637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1291) [M::mem_pestat] mean and std.dev: (427.83, 195.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (253, 384, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051) [M::mem_pestat] mean and std.dev: (395.38, 181.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1317) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (238, 312, 548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1168) [M::mem_pestat] mean and std.dev: (295.31, 134.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1478) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676396 sequences (100000220 bp) in 3.159 CPU sec, 3.527 real sec... [M::mem_process_seqs] Processed 676552 reads in 116.196 CPU sec, 3.860 real sec sorting block 42, #reads = 1080219 mem = 536879838 peak = 987090944 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282115, 0, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 231, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (247.17, 94.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (255, 386, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056) [M::mem_pestat] mean and std.dev: (397.65, 182.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1323) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 337, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797) [M::mem_pestat] mean and std.dev: (306.08, 117.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676396 reads in 113.526 CPU sec, 3.749 real sec [M::process] read 676200 sequences (100000259 bp) in 3.915 CPU sec, 4.472 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282497, 6, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 247, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1087) [M::mem_pestat] mean and std.dev: (299.56, 201.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1400) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (257, 389, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1067) [M::mem_pestat] mean and std.dev: (400.82, 184.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 309, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (256.08, 109.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676200 reads in 109.090 CPU sec, 3.622 real sec [M::process] read 676190 sequences (100000121 bp) in 3.735 CPU sec, 4.132 real sec... sorting block 43, #reads = 1078411 mem = 536879327 peak = 987090944 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 282017, 6, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 202, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (216.03, 99.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (258, 392, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1071) [M::mem_pestat] mean and std.dev: (402.70, 185.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1342) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 318, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050) [M::mem_pestat] mean and std.dev: (325.78, 157.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1333) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676190 reads in 114.348 CPU sec, 3.790 real sec [M::process] read 678598 sequences (100000219 bp) in 3.747 CPU sec, 4.171 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 282310, 0, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 262, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (264.77, 108.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (242, 378, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1067) [M::mem_pestat] mean and std.dev: (389.48, 183.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1342) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 248, 624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1476) [M::mem_pestat] mean and std.dev: (350.81, 241.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1902) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 678598 reads in 111.863 CPU sec, 3.726 real sec sorting block 44, #reads = 1078291 mem = 536879979 peak = 987090944 [M::process] read 678622 sequences (100000103 bp) in 3.799 CPU sec, 4.192 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 282721, 4, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 353, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (323.97, 125.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 380, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068) [M::mem_pestat] mean and std.dev: (390.62, 183.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1343) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 182, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (224.09, 98.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 678622 reads in 115.243 CPU sec, 3.843 real sec [M::process] read 677840 sequences (100000288 bp) in 4.003 CPU sec, 4.484 real sec... sorting block 45, #reads = 1079321 mem = 536884236 peak = 987090944 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282851, 0, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 270, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (269.46, 111.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 385, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068) [M::mem_pestat] mean and std.dev: (395.36, 184.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 274, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (296.64, 111.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677840 reads in 110.808 CPU sec, 3.674 real sec [M::process] read 677432 sequences (100000160 bp) in 3.509 CPU sec, 3.808 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282236, 1, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 559, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1261) [M::mem_pestat] mean and std.dev: (396.57, 199.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (253, 391, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1078) [M::mem_pestat] mean and std.dev: (399.74, 185.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1353) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 464, 1069) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2793) [M::mem_pestat] mean and std.dev: (346.83, 217.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3655) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677432 reads in 116.060 CPU sec, 3.902 real sec [M::process] read 677166 sequences (100000276 bp) in 3.940 CPU sec, 4.566 real sec... sorting block 46, #reads = 1079778 mem = 536879876 peak = 987090944 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 282366, 2, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 236, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (270.57, 127.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (254, 390, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076) [M::mem_pestat] mean and std.dev: (400.09, 185.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1350) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (317, 621, 4975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14291) [M::mem_pestat] mean and std.dev: (1615.27, 2177.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18949) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677166 reads in 110.557 CPU sec, 3.696 real sec [M::process] read 676628 sequences (100000119 bp) in 3.736 CPU sec, 4.120 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 281810, 3, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 287, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054) [M::mem_pestat] mean and std.dev: (265.83, 110.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1346) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (258, 397, 535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1089) [M::mem_pestat] mean and std.dev: (405.75, 186.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1366) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (264, 306, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (142, 447) [M::mem_pestat] mean and std.dev: (283.86, 55.30) [M::mem_pestat] low and high boundaries for proper pairs: (63, 508) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676628 reads in 111.700 CPU sec, 3.731 real sec sorting block 47, #reads = 1079075 mem = 536879160 peak = 987090944 [M::process] read 676438 sequences (100000244 bp) in 4.031 CPU sec, 4.468 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 282026, 9, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (266, 394, 478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (347.57, 131.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (260, 400, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1091) [M::mem_pestat] mean and std.dev: (407.23, 186.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1368) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 195, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1320) [M::mem_pestat] mean and std.dev: (308.04, 214.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1711) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676438 reads in 114.119 CPU sec, 3.808 real sec [M::process] read 676188 sequences (100000194 bp) in 3.807 CPU sec, 4.161 real sec... sorting block 48, #reads = 1080032 mem = 536878350 peak = 987090944 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282037, 1, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 276, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017) [M::mem_pestat] mean and std.dev: (312.37, 149.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1292) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (263, 403, 542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1100) [M::mem_pestat] mean and std.dev: (410.56, 187.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 600, 626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1480) [M::mem_pestat] mean and std.dev: (385.79, 212.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1907) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676188 reads in 109.461 CPU sec, 3.651 real sec [M::process] read 676168 sequences (100000192 bp) in 3.778 CPU sec, 4.184 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 282225, 3, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 260, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (264.53, 104.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (264, 404, 540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1092) [M::mem_pestat] mean and std.dev: (410.03, 186.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1368) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (302, 359, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (359.55, 100.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 994) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676168 reads in 109.619 CPU sec, 3.678 real sec [M::process] read 675650 sequences (100000081 bp) in 3.829 CPU sec, 4.230 real sec... sorting block 49, #reads = 1079265 mem = 536877958 peak = 987090944 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 281841, 7, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 340, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1065) [M::mem_pestat] mean and std.dev: (339.10, 156.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1356) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (267, 408, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1101) [M::mem_pestat] mean and std.dev: (414.24, 187.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (355, 467, 616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1138) [M::mem_pestat] mean and std.dev: (466.14, 148.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1399) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 675650 reads in 106.369 CPU sec, 3.573 real sec [M::process] read 675472 sequences (100000154 bp) in 3.669 CPU sec, 4.171 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 281444, 1, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 300, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (356.94, 193.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1155) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (270, 413, 554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1122) [M::mem_pestat] mean and std.dev: (420.10, 191.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1406) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 613, 812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2108) [M::mem_pestat] mean and std.dev: (428.14, 253.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2756) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 50, #reads = 1078567 mem = 536878382 peak = 987090944 [M::mem_process_seqs] Processed 675472 reads in 112.686 CPU sec, 3.783 real sec [M::process] read 675550 sequences (100000100 bp) in 3.808 CPU sec, 4.204 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 281727, 4, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 389, 602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1354) [M::mem_pestat] mean and std.dev: (422.10, 279.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1730) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (271, 415, 552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1114) [M::mem_pestat] mean and std.dev: (419.69, 189.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1395) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 305, 1597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4335) [M::mem_pestat] mean and std.dev: (627.33, 636.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5704) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 675550 reads in 114.992 CPU sec, 3.848 real sec [M::process] read 675654 sequences (100000150 bp) in 3.688 CPU sec, 4.105 real sec... sorting block 51, #reads = 1080368 mem = 536877678 peak = 987090944 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 281628, 2, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (258, 392, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999) [M::mem_pestat] mean and std.dev: (399.91, 206.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1246) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (269, 410, 550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1112) [M::mem_pestat] mean and std.dev: (417.30, 189.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1393) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (314, 618, 936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2180) [M::mem_pestat] mean and std.dev: (610.87, 298.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2802) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 675654 reads in 113.893 CPU sec, 3.819 real sec [M::process] read 676708 sequences (100000136 bp) in 3.523 CPU sec, 3.891 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 281621, 3, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 298, 735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1865) [M::mem_pestat] mean and std.dev: (382.53, 264.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2430) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (258, 400, 540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1104) [M::mem_pestat] mean and std.dev: (408.12, 188.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1386) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 378, 625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1543) [M::mem_pestat] mean and std.dev: (368.39, 192.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2002) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676708 reads in 118.775 CPU sec, 3.976 real sec sorting block 52, #reads = 1078980 mem = 536878625 peak = 987090944 [M::process] read 677394 sequences (100000154 bp) in 4.288 CPU sec, 4.712 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282651, 1, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 244, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (258.86, 128.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (251, 391, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1097) [M::mem_pestat] mean and std.dev: (401.57, 187.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (269, 374, 592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1238) [M::mem_pestat] mean and std.dev: (410.74, 241.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1561) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677394 reads in 117.952 CPU sec, 3.967 real sec [M::process] read 676842 sequences (100000166 bp) in 3.542 CPU sec, 3.976 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282109, 1, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 264, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (256.61, 72.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (257, 397, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1100) [M::mem_pestat] mean and std.dev: (405.97, 187.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1381) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 291, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054) [M::mem_pestat] mean and std.dev: (303.05, 145.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1352) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 53, #reads = 1078093 mem = 536879211 peak = 987090944 [M::mem_process_seqs] Processed 676842 reads in 114.384 CPU sec, 3.836 real sec [M::process] read 676620 sequences (100000200 bp) in 4.118 CPU sec, 4.567 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 281852, 2, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 357, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (284.35, 99.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (257, 396, 535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1091) [M::mem_pestat] mean and std.dev: (405.11, 186.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1369) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (262, 311, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (335.79, 146.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676664 sequences (100000175 bp) in 3.304 CPU sec, 3.677 real sec... [M::mem_process_seqs] Processed 676620 reads in 117.194 CPU sec, 3.940 real sec sorting block 54, #reads = 1080643 mem = 536878874 peak = 987090944 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 281781, 0, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (238, 310, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994) [M::mem_pestat] mean and std.dev: (356.24, 163.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1246) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (256, 396, 536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1096) [M::mem_pestat] mean and std.dev: (405.82, 188.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1376) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (246, 311, 2805) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7923) [M::mem_pestat] mean and std.dev: (1506.63, 2141.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10482) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676484 sequences (100000094 bp) in 3.479 CPU sec, 3.714 real sec... [M::mem_process_seqs] Processed 676664 reads in 115.602 CPU sec, 3.923 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281483, 4, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 235, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (264.96, 136.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1136) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (256, 394, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1087) [M::mem_pestat] mean and std.dev: (403.77, 186.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1364) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (310, 382, 959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2257) [M::mem_pestat] mean and std.dev: (496.37, 481.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2906) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 55, #reads = 1079774 mem = 536880077 peak = 1032904704 [M::process] read 676546 sequences (100000127 bp) in 3.538 CPU sec, 3.777 real sec... [M::mem_process_seqs] Processed 676484 reads in 119.334 CPU sec, 4.004 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 281401, 10, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 297, 585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1371) [M::mem_pestat] mean and std.dev: (317.07, 170.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1764) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (256, 393, 536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1096) [M::mem_pestat] mean and std.dev: (404.53, 187.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1376) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (679, 679, 823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (391, 1111) [M::mem_pestat] mean and std.dev: (743.00, 71.55) [M::mem_pestat] low and high boundaries for proper pairs: (247, 1255) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 423, 548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1210) [M::mem_pestat] mean and std.dev: (387.46, 215.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1541) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 676682 sequences (100000164 bp) in 3.330 CPU sec, 3.586 real sec... [M::mem_process_seqs] Processed 676546 reads in 117.560 CPU sec, 3.947 real sec sorting block 56, #reads = 1078996 mem = 536879385 peak = 1032904704 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 281198, 1, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 285, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923) [M::mem_pestat] mean and std.dev: (320.64, 206.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1185) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (253, 390, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1084) [M::mem_pestat] mean and std.dev: (401.29, 186.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1361) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 246, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (255.00, 118.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676948 sequences (100000062 bp) in 3.305 CPU sec, 3.497 real sec... [M::mem_process_seqs] Processed 676682 reads in 115.070 CPU sec, 3.844 real sec sorting block 57, #reads = 1079568 mem = 536879045 peak = 1036087296 [M::process] read 676830 sequences (100000295 bp) in 3.321 CPU sec, 3.510 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 282537, 3, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 343, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (294.12, 164.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1150) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (252, 388, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1080) [M::mem_pestat] mean and std.dev: (399.27, 185.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1356) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 237, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (268.39, 113.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 990) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 282076, 7, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 282, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (281.05, 134.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (252, 389, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1080) [M::mem_pestat] mean and std.dev: (399.61, 185.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1356) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 270, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1195) [M::mem_pestat] mean and std.dev: (250.68, 125.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1541) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677392 sequences (100000166 bp) in 3.122 CPU sec, 3.385 real sec... [M::mem_process_seqs] Processed 676948 reads in 212.084 CPU sec, 6.915 real sec [M::mem_process_seqs] Processed 676830 reads in 140.273 CPU sec, 4.527 real sec sorting block 58, #reads = 1079619 mem = 536878570 peak = 1036087296 [M::process] read 677700 sequences (100000060 bp) in 2.930 CPU sec, 3.084 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282231, 5, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 152, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975) [M::mem_pestat] mean and std.dev: (239.79, 141.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1256) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 384, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074) [M::mem_pestat] mean and std.dev: (395.79, 184.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1349) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 241, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (242.41, 77.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676970 sequences (100000068 bp) in 2.855 CPU sec, 3.052 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282252, 3, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 240, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975) [M::mem_pestat] mean and std.dev: (320.52, 231.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1256) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 380, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1065) [M::mem_pestat] mean and std.dev: (391.21, 182.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 373, 515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1063) [M::mem_pestat] mean and std.dev: (374.21, 187.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1337) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677392 reads in 218.359 CPU sec, 7.027 real sec [M::mem_process_seqs] Processed 677700 reads in 147.761 CPU sec, 4.707 real sec sorting block 59, #reads = 1080501 mem = 536879826 peak = 1036087296 [M::process] read 676814 sequences (100000187 bp) in 2.993 CPU sec, 3.148 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 282081, 0, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 263, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011) [M::mem_pestat] mean and std.dev: (268.34, 129.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1297) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (251, 387, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076) [M::mem_pestat] mean and std.dev: (398.18, 184.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1351) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 296, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (290.08, 138.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 60, #reads = 1078191 mem = 536877419 peak = 1131065344 [M::process] read 676488 sequences (100000061 bp) in 3.318 CPU sec, 3.689 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 282085, 0, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (229, 278, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (296.26, 122.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (254, 390, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088) [M::mem_pestat] mean and std.dev: (402.18, 187.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1366) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (297, 412, 647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1347) [M::mem_pestat] mean and std.dev: (427.52, 251.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1697) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676970 reads in 214.889 CPU sec, 6.941 real sec [M::mem_process_seqs] Processed 676814 reads in 146.351 CPU sec, 4.691 real sec [M::process] read 676816 sequences (100000106 bp) in 2.905 CPU sec, 3.075 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 282066, 7, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 264, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (245.81, 69.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (254, 391, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1094) [M::mem_pestat] mean and std.dev: (403.38, 188.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1374) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 485, 610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1450) [M::mem_pestat] mean and std.dev: (386.04, 203.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1870) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 61, #reads = 1079434 mem = 536879850 peak = 1131065344 [M::process] read 676578 sequences (100000285 bp) in 3.297 CPU sec, 3.479 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 281195, 6, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 413, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1100) [M::mem_pestat] mean and std.dev: (320.03, 143.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (254, 390, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1094) [M::mem_pestat] mean and std.dev: (402.77, 188.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1374) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 389, 540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1198) [M::mem_pestat] mean and std.dev: (357.94, 180.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1527) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676488 reads in 210.070 CPU sec, 6.745 real sec [M::mem_process_seqs] Processed 676816 reads in 139.391 CPU sec, 4.413 real sec [M::process] read 676416 sequences (100000137 bp) in 2.770 CPU sec, 2.830 real sec... sorting block 62, #reads = 1079375 mem = 536877260 peak = 1131065344 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 281724, 5, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (250, 340, 619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1357) [M::mem_pestat] mean and std.dev: (302.03, 135.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1726) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (254, 389, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1082) [M::mem_pestat] mean and std.dev: (401.67, 187.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1358) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 289, 585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1337) [M::mem_pestat] mean and std.dev: (329.00, 157.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1713) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676278 sequences (100000225 bp) in 3.740 CPU sec, 4.210 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 281637, 4, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 311, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037) [M::mem_pestat] mean and std.dev: (314.91, 155.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1320) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (256, 392, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1087) [M::mem_pestat] mean and std.dev: (403.45, 187.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1364) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 323, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (259.35, 100.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676578 reads in 207.246 CPU sec, 6.680 real sec [M::mem_process_seqs] Processed 676416 reads in 138.391 CPU sec, 4.419 real sec sorting block 63, #reads = 1079358 mem = 536880154 peak = 1131065344 [M::process] read 676496 sequences (100000301 bp) in 3.130 CPU sec, 3.321 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 281813, 4, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 252, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (276.68, 136.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (256, 391, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1087) [M::mem_pestat] mean and std.dev: (403.46, 187.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1364) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (277, 494, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045) [M::mem_pestat] mean and std.dev: (425.77, 223.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1318) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 281821, 2, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 290, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (308.28, 135.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (255, 390, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1086) [M::mem_pestat] mean and std.dev: (402.84, 187.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1363) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 234, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1175) [M::mem_pestat] mean and std.dev: (297.97, 170.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1510) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676372 sequences (100000183 bp) in 3.169 CPU sec, 3.450 real sec... [M::mem_process_seqs] Processed 676278 reads in 201.386 CPU sec, 6.490 real sec sorting block 64, #reads = 1079399 mem = 536878933 peak = 1131065344 [M::mem_process_seqs] Processed 676496 reads in 136.329 CPU sec, 4.365 real sec [M::process] read 676632 sequences (100000233 bp) in 3.171 CPU sec, 3.376 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 281752, 7, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 271, 594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1362) [M::mem_pestat] mean and std.dev: (345.52, 224.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (256, 391, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1090) [M::mem_pestat] mean and std.dev: (403.90, 187.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1368) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 305, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (345.86, 150.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 65, #reads = 1079320 mem = 536877714 peak = 1131065344 [M::process] read 676116 sequences (100000125 bp) in 3.158 CPU sec, 3.341 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282901, 4, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 293, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 500) [M::mem_pestat] mean and std.dev: (265.12, 66.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (255, 391, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1092) [M::mem_pestat] mean and std.dev: (403.78, 188.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1371) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 205, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017) [M::mem_pestat] mean and std.dev: (251.63, 136.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1306) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676372 reads in 199.087 CPU sec, 6.403 real sec [M::mem_process_seqs] Processed 676632 reads in 136.385 CPU sec, 4.361 real sec [M::process] read 676380 sequences (100000186 bp) in 2.769 CPU sec, 2.905 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 281695, 0, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 279, 649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1563) [M::mem_pestat] mean and std.dev: (343.76, 199.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2020) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (258, 395, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1095) [M::mem_pestat] mean and std.dev: (407.31, 189.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1374) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 372, 570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1288) [M::mem_pestat] mean and std.dev: (393.26, 192.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1647) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 66, #reads = 1079399 mem = 536879622 peak = 1131065344 [M::process] read 678304 sequences (100000145 bp) in 2.880 CPU sec, 3.219 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282034, 1, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 368, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1121) [M::mem_pestat] mean and std.dev: (353.64, 176.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (255, 390, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074) [M::mem_pestat] mean and std.dev: (401.28, 185.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1347) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 287, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (291.00, 135.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676116 reads in 203.255 CPU sec, 6.587 real sec [M::mem_process_seqs] Processed 676380 reads in 142.654 CPU sec, 4.581 real sec [M::process] read 677564 sequences (100000220 bp) in 3.034 CPU sec, 3.160 real sec... sorting block 67, #reads = 1079455 mem = 536879391 peak = 1131065344 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282357, 1, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 254, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837) [M::mem_pestat] mean and std.dev: (225.15, 87.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1063) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 379, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1069) [M::mem_pestat] mean and std.dev: (391.12, 183.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1344) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 256, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (295.05, 107.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677144 sequences (100000064 bp) in 3.128 CPU sec, 3.443 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 281961, 2, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 257, 536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1156) [M::mem_pestat] mean and std.dev: (346.10, 175.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (250, 388, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1087) [M::mem_pestat] mean and std.dev: (399.16, 186.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1366) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 358, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026) [M::mem_pestat] mean and std.dev: (338.14, 136.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1291) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 678304 reads in 209.937 CPU sec, 6.770 real sec [M::mem_process_seqs] Processed 677564 reads in 142.178 CPU sec, 4.547 real sec sorting block 68, #reads = 1078417 mem = 536879711 peak = 1131065344 [M::process] read 676990 sequences (100000135 bp) in 2.878 CPU sec, 3.011 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 281840, 4, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 318, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (289.13, 122.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (254, 392, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088) [M::mem_pestat] mean and std.dev: (402.28, 187.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1366) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (298, 298, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (256, 361) [M::mem_pestat] mean and std.dev: (301.08, 15.45) [M::mem_pestat] low and high boundaries for proper pairs: (235, 382) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676644 sequences (100000090 bp) in 3.101 CPU sec, 3.535 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281742, 8, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 375, 653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1637) [M::mem_pestat] mean and std.dev: (390.41, 211.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2129) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (255, 395, 536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1098) [M::mem_pestat] mean and std.dev: (405.01, 188.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (299, 388, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (360.10, 102.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1019) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677144 reads in 208.490 CPU sec, 6.746 real sec sorting block 69, #reads = 1081468 mem = 536877526 peak = 1131065344 [M::mem_process_seqs] Processed 676990 reads in 141.735 CPU sec, 4.552 real sec [M::process] read 676318 sequences (100000036 bp) in 2.539 CPU sec, 2.669 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 281076, 3, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 234, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (342.62, 227.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1253) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (258, 399, 541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1107) [M::mem_pestat] mean and std.dev: (408.42, 189.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1390) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (304, 410, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (378.71, 131.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 70, #reads = 1080413 mem = 536881276 peak = 1131065344 [M::process] read 676354 sequences (100000087 bp) in 3.123 CPU sec, 3.424 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 281402, 2, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 290, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (268.00, 117.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (262, 404, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1111) [M::mem_pestat] mean and std.dev: (412.10, 189.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1394) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 338, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (322.17, 131.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676644 reads in 213.038 CPU sec, 6.848 real sec [M::process] read 676212 sequences (100000112 bp) in 2.336 CPU sec, 2.445 real sec... [M::mem_process_seqs] Processed 676318 reads in 144.089 CPU sec, 4.607 real sec sorting block 71, #reads = 1080702 mem = 536877799 peak = 1131065344 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 281644, 8, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 288, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959) [M::mem_pestat] mean and std.dev: (317.89, 149.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1212) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (264, 405, 547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1113) [M::mem_pestat] mean and std.dev: (412.93, 189.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1396) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (249, 332, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960) [M::mem_pestat] mean and std.dev: (335.38, 118.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1197) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 675714 sequences (100000175 bp) in 3.339 CPU sec, 3.702 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 281356, 3, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 415, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041) [M::mem_pestat] mean and std.dev: (296.56, 137.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1332) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (261, 403, 544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1110) [M::mem_pestat] mean and std.dev: (411.94, 190.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1393) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 332, 599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1367) [M::mem_pestat] mean and std.dev: (375.00, 178.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1751) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676354 reads in 206.455 CPU sec, 6.639 real sec [M::mem_process_seqs] Processed 676212 reads in 138.648 CPU sec, 4.412 real sec [M::process] read 675400 sequences (100000108 bp) in 2.858 CPU sec, 2.972 real sec... sorting block 72, #reads = 1081368 mem = 536878499 peak = 1131065344 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 281153, 6, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (222, 359, 556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1224) [M::mem_pestat] mean and std.dev: (373.17, 235.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (266, 407, 547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1109) [M::mem_pestat] mean and std.dev: (414.65, 189.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1390) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 312, 580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1318) [M::mem_pestat] mean and std.dev: (347.15, 169.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1687) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 675214 sequences (100000287 bp) in 3.445 CPU sec, 3.857 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 281309, 2, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (291, 441, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (412.83, 160.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1075) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (269, 411, 550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1112) [M::mem_pestat] mean and std.dev: (417.31, 189.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1393) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 359, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1237) [M::mem_pestat] mean and std.dev: (345.71, 199.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1595) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 675714 reads in 213.803 CPU sec, 6.881 real sec [M::process] read 675452 sequences (100000286 bp) in 2.007 CPU sec, 2.082 real sec... [M::mem_process_seqs] Processed 675400 reads in 143.278 CPU sec, 4.565 real sec sorting block 73, #reads = 1080275 mem = 536880732 peak = 1131065344 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 281322, 6, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 317, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1120) [M::mem_pestat] mean and std.dev: (371.59, 206.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (271, 413, 550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1108) [M::mem_pestat] mean and std.dev: (418.85, 188.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1387) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 347, 1166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3124) [M::mem_pestat] mean and std.dev: (431.41, 435.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4103) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 74, #reads = 1079298 mem = 536880049 peak = 1131065344 [M::process] read 675194 sequences (100000020 bp) in 3.343 CPU sec, 3.662 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 280958, 7, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 302, 813) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2013) [M::mem_pestat] mean and std.dev: (347.93, 211.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (272, 413, 552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1112) [M::mem_pestat] mean and std.dev: (419.61, 189.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1392) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 287, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (299.57, 135.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1081) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 675214 reads in 209.727 CPU sec, 6.757 real sec [M::mem_process_seqs] Processed 675452 reads in 142.034 CPU sec, 4.519 real sec [M::process] read 676744 sequences (100000058 bp) in 3.060 CPU sec, 3.229 real sec... sorting block 75, #reads = 1079659 mem = 536878171 peak = 1131065344 [M::process] read 676888 sequences (100000192 bp) in 3.277 CPU sec, 3.534 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 281668, 3, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 352, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984) [M::mem_pestat] mean and std.dev: (323.00, 169.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1256) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (258, 401, 542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1110) [M::mem_pestat] mean and std.dev: (409.10, 189.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1394) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 318, 565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1375) [M::mem_pestat] mean and std.dev: (274.17, 164.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1780) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676744 reads in 94.728 CPU sec, 2.984 real sec [M::process] read 676326 sequences (100000215 bp) in 1.955 CPU sec, 1.989 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 280628, 3, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (269, 346, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (73, 563) [M::mem_pestat] mean and std.dev: (301.62, 88.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (274, 419, 557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1123) [M::mem_pestat] mean and std.dev: (423.42, 190.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1406) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (260, 323, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028) [M::mem_pestat] mean and std.dev: (388.62, 207.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1284) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 281463, 4, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 332, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (300.25, 147.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (255, 398, 540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1110) [M::mem_pestat] mean and std.dev: (406.60, 188.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1395) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 332, 605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1355) [M::mem_pestat] mean and std.dev: (347.00, 178.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1730) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 675194 reads in 278.096 CPU sec, 8.865 real sec sorting block 76, #reads = 1079933 mem = 536879140 peak = 1131065344 [M::mem_process_seqs] Processed 676888 reads in 122.696 CPU sec, 3.873 real sec [M::process] read 676368 sequences (100000020 bp) in 3.035 CPU sec, 3.105 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 281471, 10, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (247, 391, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033) [M::mem_pestat] mean and std.dev: (394.42, 210.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1295) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (259, 402, 542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1108) [M::mem_pestat] mean and std.dev: (409.42, 188.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1391) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (347, 557, 648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1250) [M::mem_pestat] mean and std.dev: (506.50, 192.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1551) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (265, 349, 936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2278) [M::mem_pestat] mean and std.dev: (390.05, 245.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2949) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 77, #reads = 1079979 mem = 536877394 peak = 1131065344 [M::process] read 676622 sequences (100000048 bp) in 2.883 CPU sec, 3.013 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 281236, 1, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 328, 566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1248) [M::mem_pestat] mean and std.dev: (370.08, 187.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (258, 400, 540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1104) [M::mem_pestat] mean and std.dev: (408.48, 188.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1386) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 560, 653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1559) [M::mem_pestat] mean and std.dev: (416.35, 213.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2012) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676326 reads in 165.158 CPU sec, 5.264 real sec [M::mem_process_seqs] Processed 676368 reads in 109.623 CPU sec, 3.461 real sec [M::process] read 676538 sequences (100000117 bp) in 3.022 CPU sec, 3.182 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 280996, 2, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 173, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (191.59, 80.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (257, 397, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1100) [M::mem_pestat] mean and std.dev: (405.79, 187.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1381) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (343, 389, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (139, 649) [M::mem_pestat] mean and std.dev: (365.69, 91.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 78, #reads = 1079445 mem = 536878403 peak = 1131065344 [M::process] read 676824 sequences (100000301 bp) in 3.388 CPU sec, 3.605 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 280576, 1, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 391, 799) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1945) [M::mem_pestat] mean and std.dev: (427.83, 258.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (254, 393, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088) [M::mem_pestat] mean and std.dev: (402.18, 185.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1366) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 315, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (321.15, 125.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676622 reads in 221.280 CPU sec, 7.091 real sec [M::mem_process_seqs] Processed 676538 reads in 148.610 CPU sec, 4.719 real sec [M::process] read 676974 sequences (100000283 bp) in 2.737 CPU sec, 2.818 real sec... sorting block 79, #reads = 1079474 mem = 536878247 peak = 1131065344 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 281442, 4, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (319, 409, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (61, 706) [M::mem_pestat] mean and std.dev: (368.32, 145.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (253, 391, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1084) [M::mem_pestat] mean and std.dev: (400.47, 184.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1361) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 347, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1098) [M::mem_pestat] mean and std.dev: (386.64, 242.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1385) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676860 sequences (100000298 bp) in 3.255 CPU sec, 3.413 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 281720, 7, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 264, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (276.50, 108.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (252, 389, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1083) [M::mem_pestat] mean and std.dev: (399.34, 184.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1360) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (288, 387, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (345.89, 124.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676824 reads in 214.290 CPU sec, 6.862 real sec [M::mem_process_seqs] Processed 676974 reads in 142.877 CPU sec, 4.517 real sec [M::process] read 676880 sequences (100000146 bp) in 2.714 CPU sec, 2.779 real sec... sorting block 80, #reads = 1078902 mem = 536883138 peak = 1131065344 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 281141, 0, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 193, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931) [M::mem_pestat] mean and std.dev: (237.82, 127.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1197) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (252, 387, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074) [M::mem_pestat] mean and std.dev: (397.45, 182.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1348) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 279, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1175) [M::mem_pestat] mean and std.dev: (331.75, 202.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1500) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676898 sequences (100000168 bp) in 3.230 CPU sec, 3.393 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 281459, 2, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 182, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 366) [M::mem_pestat] mean and std.dev: (182.07, 47.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (253, 388, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1072) [M::mem_pestat] mean and std.dev: (397.83, 182.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1345) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 322, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1100) [M::mem_pestat] mean and std.dev: (346.30, 178.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1401) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676602 sequences (100000079 bp) in 1.740 CPU sec, 1.740 real sec... [M::mem_process_seqs] Processed 676860 reads in 192.261 CPU sec, 6.130 real sec sorting block 81, #reads = 1079683 mem = 536881726 peak = 1152262144 [M::mem_process_seqs] Processed 676880 reads in 118.514 CPU sec, 3.760 real sec sorting block 82, #reads = 1078877 mem = 536879796 peak = 1152262144 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 281272, 1, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 269, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (278.00, 118.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (251, 387, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1073) [M::mem_pestat] mean and std.dev: (396.41, 182.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1347) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (315, 377, 1302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3276) [M::mem_pestat] mean and std.dev: (761.39, 747.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4263) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676922 sequences (100000263 bp) in 3.062 CPU sec, 3.203 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 281397, 2, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 257, 573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1407) [M::mem_pestat] mean and std.dev: (369.62, 256.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1824) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (254, 389, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076) [M::mem_pestat] mean and std.dev: (399.03, 183.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1350) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (379, 477, 2263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6031) [M::mem_pestat] mean and std.dev: (1427.81, 1898.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9021) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676898 reads in 176.909 CPU sec, 5.600 real sec [M::mem_process_seqs] Processed 676602 reads in 115.335 CPU sec, 3.628 real sec [M::process] read 676630 sequences (100000021 bp) in 2.602 CPU sec, 2.630 real sec... sorting block 83, #reads = 1079296 mem = 536880105 peak = 1152262144 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281220, 1, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 204, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (290.27, 156.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (254, 391, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1082) [M::mem_pestat] mean and std.dev: (400.05, 184.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1358) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 256, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (288.07, 143.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676954 sequences (100000248 bp) in 3.315 CPU sec, 3.473 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 281120, 0, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 273, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041) [M::mem_pestat] mean and std.dev: (304.33, 167.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1318) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (252, 389, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1089) [M::mem_pestat] mean and std.dev: (399.79, 184.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1368) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (262, 339, 584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1228) [M::mem_pestat] mean and std.dev: (387.12, 214.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1550) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676922 reads in 178.457 CPU sec, 5.676 real sec [M::mem_process_seqs] Processed 676630 reads in 119.342 CPU sec, 3.756 real sec [M::process] read 676642 sequences (100000224 bp) in 2.771 CPU sec, 2.819 real sec... sorting block 84, #reads = 1080091 mem = 536879557 peak = 1152262144 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 281946, 2, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 219, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (217.72, 79.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (252, 389, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1092) [M::mem_pestat] mean and std.dev: (400.38, 185.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1372) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 234, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (261.97, 100.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676812 sequences (100000000 bp) in 3.004 CPU sec, 3.138 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 281347, 13, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (279, 382, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (354.77, 162.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1006) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (253, 391, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093) [M::mem_pestat] mean and std.dev: (401.75, 186.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1373) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (2267, 2267, 2527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1747, 3047) [M::mem_pestat] mean and std.dev: (2337.91, 115.79) [M::mem_pestat] low and high boundaries for proper pairs: (1487, 3307) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 309, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (303.86, 114.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676954 reads in 175.654 CPU sec, 5.580 real sec [M::mem_process_seqs] Processed 676642 reads in 115.810 CPU sec, 3.646 real sec sorting block 85, #reads = 1079504 mem = 536877596 peak = 1152262144 [M::process] read 676410 sequences (100000157 bp) in 2.873 CPU sec, 2.918 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 281743, 5, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (282, 397, 5202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15042) [M::mem_pestat] mean and std.dev: (1713.24, 2197.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19962) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (251, 385, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1073) [M::mem_pestat] mean and std.dev: (396.44, 183.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1347) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (286, 384, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931) [M::mem_pestat] mean and std.dev: (386.15, 139.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676400 sequences (100000262 bp) in 2.984 CPU sec, 3.106 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 281442, 3, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 314, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968) [M::mem_pestat] mean and std.dev: (298.88, 142.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1216) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (254, 390, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1073) [M::mem_pestat] mean and std.dev: (399.49, 183.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1346) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 271, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (254.40, 118.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676812 reads in 173.622 CPU sec, 5.518 real sec sorting block 86, #reads = 1079376 mem = 536879525 peak = 1152262144 [M::mem_process_seqs] Processed 676410 reads in 116.769 CPU sec, 3.687 real sec [M::process] read 676178 sequences (100000079 bp) in 2.841 CPU sec, 2.899 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 282330, 1, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 212, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972) [M::mem_pestat] mean and std.dev: (253.94, 156.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1253) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (256, 391, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1072) [M::mem_pestat] mean and std.dev: (400.35, 183.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1344) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 319, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1187) [M::mem_pestat] mean and std.dev: (340.00, 190.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1514) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 87, #reads = 1078363 mem = 536878226 peak = 1152262144 [M::process] read 675908 sequences (100000159 bp) in 3.049 CPU sec, 3.187 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281342, 1, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 262, 539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1279) [M::mem_pestat] mean and std.dev: (326.54, 230.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (258, 392, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074) [M::mem_pestat] mean and std.dev: (401.63, 183.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1346) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (271, 363, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (364.29, 134.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676400 reads in 174.138 CPU sec, 5.550 real sec [M::mem_process_seqs] Processed 676178 reads in 115.711 CPU sec, 3.651 real sec [M::process] read 676022 sequences (100000056 bp) in 2.807 CPU sec, 2.894 real sec... sorting block 88, #reads = 1080114 mem = 536878205 peak = 1152262144 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282010, 3, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 258, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1058) [M::mem_pestat] mean and std.dev: (290.83, 133.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1352) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (260, 394, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076) [M::mem_pestat] mean and std.dev: (403.68, 183.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1348) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 305, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (276.12, 85.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 675808 sequences (100000008 bp) in 2.891 CPU sec, 3.006 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282063, 2, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 328, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (302.81, 108.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (258, 392, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074) [M::mem_pestat] mean and std.dev: (402.65, 184.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1346) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 307, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1069) [M::mem_pestat] mean and std.dev: (317.27, 141.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1350) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 675908 reads in 167.575 CPU sec, 5.338 real sec [M::mem_process_seqs] Processed 676022 reads in 110.941 CPU sec, 3.506 real sec [M::process] read 675938 sequences (100000099 bp) in 2.529 CPU sec, 2.587 real sec... sorting block 89, #reads = 1078637 mem = 536883372 peak = 1152262144 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 282248, 2, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 239, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (259.29, 146.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (261, 395, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1080) [M::mem_pestat] mean and std.dev: (405.39, 184.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1353) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 257, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (261.77, 84.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681772 sequences (100000146 bp) in 2.677 CPU sec, 2.805 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282103, 2, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 240, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (282.73, 149.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (260, 395, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076) [M::mem_pestat] mean and std.dev: (404.70, 184.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1348) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 265, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (267.55, 80.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 675808 reads in 162.138 CPU sec, 5.171 real sec [M::mem_process_seqs] Processed 675938 reads in 107.187 CPU sec, 3.381 real sec sorting block 90, #reads = 1079289 mem = 536882421 peak = 1152262144 [M::process] read 681712 sequences (100000258 bp) in 3.273 CPU sec, 3.533 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 284317, 4, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 347, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (293.73, 144.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (221, 357, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1067) [M::mem_pestat] mean and std.dev: (371.85, 181.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1349) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 302, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (313.04, 130.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1021) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 91, #reads = 1079521 mem = 536878556 peak = 1152262144 [M::process] read 681202 sequences (100000193 bp) in 3.022 CPU sec, 3.115 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283938, 0, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 255, 637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1597) [M::mem_pestat] mean and std.dev: (256.96, 170.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2077) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (223, 362, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1081) [M::mem_pestat] mean and std.dev: (375.45, 182.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1367) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (238, 270, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (288.73, 94.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681772 reads in 185.779 CPU sec, 5.934 real sec [M::mem_process_seqs] Processed 681712 reads in 123.686 CPU sec, 3.906 real sec [M::process] read 681002 sequences (100000184 bp) in 2.772 CPU sec, 2.863 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 283279, 1, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 270, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (229.00, 83.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (225, 366, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1089) [M::mem_pestat] mean and std.dev: (378.27, 183.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1377) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 232, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970) [M::mem_pestat] mean and std.dev: (267.98, 141.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1240) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 92, #reads = 1078373 mem = 536881099 peak = 1152262144 [M::process] read 681208 sequences (100000279 bp) in 2.963 CPU sec, 3.068 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 283713, 5, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 347, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (277.18, 112.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (227, 369, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1085) [M::mem_pestat] mean and std.dev: (379.20, 181.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1371) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (302, 520, 4028) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11480) [M::mem_pestat] mean and std.dev: (1681.75, 2035.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15206) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681202 reads in 185.351 CPU sec, 5.893 real sec [M::mem_process_seqs] Processed 681002 reads in 122.780 CPU sec, 3.870 real sec [M::process] read 680320 sequences (100000112 bp) in 2.490 CPU sec, 2.531 real sec... sorting block 93, #reads = 1078726 mem = 536877935 peak = 1152262144 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 283595, 2, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 271, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1107) [M::mem_pestat] mean and std.dev: (275.30, 160.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (225, 364, 506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068) [M::mem_pestat] mean and std.dev: (374.14, 178.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1349) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 322, 548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1262) [M::mem_pestat] mean and std.dev: (310.60, 157.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1619) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679746 sequences (100000163 bp) in 2.882 CPU sec, 3.012 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 283351, 2, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 354, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848) [M::mem_pestat] mean and std.dev: (336.40, 169.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1069) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (231, 372, 512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074) [M::mem_pestat] mean and std.dev: (380.25, 179.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 285, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (266.65, 121.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1017) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679046 sequences (100000246 bp) in 2.110 CPU sec, 2.108 real sec... [M::mem_process_seqs] Processed 681208 reads in 192.727 CPU sec, 6.113 real sec [M::mem_process_seqs] Processed 680320 reads in 124.126 CPU sec, 3.926 real sec sorting block 94, #reads = 1077292 mem = 536880266 peak = 1152262144 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 282614, 2, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 269, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (274.83, 99.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (234, 377, 515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1077) [M::mem_pestat] mean and std.dev: (383.62, 179.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1358) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (258, 346, 611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1317) [M::mem_pestat] mean and std.dev: (392.38, 215.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1670) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678930 sequences (100000156 bp) in 2.888 CPU sec, 3.045 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282323, 0, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 367, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1105) [M::mem_pestat] mean and std.dev: (326.38, 159.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (240, 383, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1077) [M::mem_pestat] mean and std.dev: (387.80, 179.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1356) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 306, 591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1433) [M::mem_pestat] mean and std.dev: (292.89, 168.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1854) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678752 sequences (100000004 bp) in 2.310 CPU sec, 2.307 real sec... [M::mem_process_seqs] Processed 679746 reads in 184.469 CPU sec, 5.837 real sec sorting block 95, #reads = 1075629 mem = 536879002 peak = 1152262144 [M::mem_process_seqs] Processed 679046 reads in 116.564 CPU sec, 3.676 real sec sorting block 96, #reads = 1074746 mem = 536877407 peak = 1152262144 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 282769, 9, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 346, 699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1779) [M::mem_pestat] mean and std.dev: (344.39, 208.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2319) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (242, 385, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076) [M::mem_pestat] mean and std.dev: (389.78, 180.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1354) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 333, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (319.79, 90.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679776 sequences (100000163 bp) in 3.178 CPU sec, 3.268 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282890, 5, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 320, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990) [M::mem_pestat] mean and std.dev: (290.96, 148.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1274) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 385, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1077) [M::mem_pestat] mean and std.dev: (390.53, 180.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 283, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (296.55, 101.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 678930 reads in 185.930 CPU sec, 5.882 real sec [M::process] read 680792 sequences (100000001 bp) in 2.601 CPU sec, 2.613 real sec... [M::process] read 681656 sequences (100000057 bp) in 1.879 CPU sec, 1.886 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 283588, 0, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 250, 591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1463) [M::mem_pestat] mean and std.dev: (317.66, 192.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1899) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (228, 365, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1059) [M::mem_pestat] mean and std.dev: (374.84, 177.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1336) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 264, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (263.12, 124.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680792 reads in 101.964 CPU sec, 3.417 real sec sorting block 97, #reads = 1075954 mem = 536877808 peak = 1152262144 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 283477, 2, 45) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (234, 373, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1059) [M::mem_pestat] mean and std.dev: (380.10, 177.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 297, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1194) [M::mem_pestat] mean and std.dev: (323.86, 185.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1536) [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 679776 reads in 205.589 CPU sec, 7.149 real sec [M::mem_process_seqs] Processed 678752 reads in 384.790 CPU sec, 12.785 real sec sorting block 98, #reads = 1078106 mem = 536878750 peak = 1152262144 [M::process] read 681252 sequences (100000013 bp) in 2.484 CPU sec, 2.580 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 284756, 5, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 345, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1077) [M::mem_pestat] mean and std.dev: (299.50, 143.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1381) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (224, 359, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052) [M::mem_pestat] mean and std.dev: (370.38, 176.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1328) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 250, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (299.85, 134.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681188 sequences (100000201 bp) in 2.752 CPU sec, 2.870 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 283987, 0, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (352, 359, 575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021) [M::mem_pestat] mean and std.dev: (359.00, 134.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1244) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (225, 361, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053) [M::mem_pestat] mean and std.dev: (371.13, 175.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (273, 492, 972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2370) [M::mem_pestat] mean and std.dev: (393.08, 170.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3069) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681656 reads in 219.258 CPU sec, 6.911 real sec [M::mem_process_seqs] Processed 681252 reads in 134.053 CPU sec, 4.229 real sec sorting block 99, #reads = 1077938 mem = 536879167 peak = 1152262144 [M::process] read 681384 sequences (100000193 bp) in 2.967 CPU sec, 3.020 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 283723, 7, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 208, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (225.38, 95.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (226, 363, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060) [M::mem_pestat] mean and std.dev: (372.55, 176.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 244, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (293.44, 150.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680036 sequences (100000191 bp) in 3.258 CPU sec, 3.462 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 283635, 9, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (246, 368, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (310.46, 111.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (224, 362, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055) [M::mem_pestat] mean and std.dev: (370.86, 175.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1332) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 307, 512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1194) [M::mem_pestat] mean and std.dev: (327.49, 184.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1535) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681188 reads in 206.249 CPU sec, 6.547 real sec sorting block 100, #reads = 1075579 mem = 536874362 peak = 1152262144 [M::process] read 679338 sequences (100000236 bp) in 2.610 CPU sec, 2.638 real sec... [M::mem_process_seqs] Processed 681384 reads in 135.742 CPU sec, 4.273 real sec sorting block 101, #reads = 1075262 mem = 536876521 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 282862, 4, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 264, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (282.11, 109.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1163) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (233, 371, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1064) [M::mem_pestat] mean and std.dev: (379.57, 177.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 344, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (324.37, 128.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679264 sequences (100000034 bp) in 3.402 CPU sec, 3.546 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 282955, 1, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 301, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (270.69, 101.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (237, 374, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068) [M::mem_pestat] mean and std.dev: (382.77, 177.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1345) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (300, 379, 630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1290) [M::mem_pestat] mean and std.dev: (459.38, 302.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1671) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680520 sequences (100000233 bp) in 2.410 CPU sec, 2.404 real sec... [M::mem_process_seqs] Processed 680036 reads in 206.645 CPU sec, 6.542 real sec [M::mem_process_seqs] Processed 679338 reads in 135.165 CPU sec, 4.268 real sec sorting block 102, #reads = 1075254 mem = 536877186 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 283203, 2, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 527, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1179) [M::mem_pestat] mean and std.dev: (410.80, 199.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (238, 375, 512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060) [M::mem_pestat] mean and std.dev: (383.02, 177.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 357, 640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1540) [M::mem_pestat] mean and std.dev: (424.55, 306.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1990) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682476 sequences (100000265 bp) in 2.801 CPU sec, 2.897 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 283564, 4, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 305, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1305) [M::mem_pestat] mean and std.dev: (262.55, 120.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (230, 363, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040) [M::mem_pestat] mean and std.dev: (372.27, 173.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1310) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 344, 572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1330) [M::mem_pestat] mean and std.dev: (314.30, 165.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1709) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680240 sequences (100000070 bp) in 2.450 CPU sec, 2.454 real sec... [M::mem_process_seqs] Processed 679264 reads in 195.528 CPU sec, 6.188 real sec [M::mem_process_seqs] Processed 680520 reads in 130.965 CPU sec, 4.136 real sec sorting block 103, #reads = 1075832 mem = 536879120 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 284783, 3, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 276, 685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1737) [M::mem_pestat] mean and std.dev: (297.90, 187.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2263) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (219, 350, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017) [M::mem_pestat] mean and std.dev: (360.50, 170.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1283) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 312, 544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1222) [M::mem_pestat] mean and std.dev: (308.39, 142.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1561) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679824 sequences (100000078 bp) in 3.543 CPU sec, 3.769 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 283941, 5, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 336, 600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1444) [M::mem_pestat] mean and std.dev: (308.89, 194.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1866) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (230, 362, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028) [M::mem_pestat] mean and std.dev: (370.15, 170.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1294) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 330, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975) [M::mem_pestat] mean and std.dev: (313.23, 122.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1230) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678894 sequences (100000139 bp) in 2.500 CPU sec, 2.498 real sec... [M::mem_process_seqs] Processed 682476 reads in 209.508 CPU sec, 6.621 real sec sorting block 104, #reads = 1071115 mem = 536879937 peak = 1169416192 [M::mem_process_seqs] Processed 680240 reads in 135.522 CPU sec, 4.285 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 283054, 0, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 318, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (291.69, 110.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (232, 366, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045) [M::mem_pestat] mean and std.dev: (374.86, 173.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1316) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 286, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (269.85, 108.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 105, #reads = 1070929 mem = 536880450 peak = 1169416192 [M::process] read 679116 sequences (100000229 bp) in 3.084 CPU sec, 3.295 real sec... [M::process] read 678722 sequences (100000077 bp) in 1.840 CPU sec, 1.832 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 283080, 4, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 215, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020) [M::mem_pestat] mean and std.dev: (286.73, 170.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1314) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (238, 374, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051) [M::mem_pestat] mean and std.dev: (381.48, 175.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1322) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 361, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (315.74, 160.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1069) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 679824 reads in 202.647 CPU sec, 6.439 real sec [M::mem_process_seqs] Processed 678894 reads in 131.760 CPU sec, 4.179 real sec sorting block 106, #reads = 1072065 mem = 536877338 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 283551, 4, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (242, 312, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995) [M::mem_pestat] mean and std.dev: (352.11, 189.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1246) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (236, 372, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055) [M::mem_pestat] mean and std.dev: (380.37, 175.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1328) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (264, 517, 536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1080) [M::mem_pestat] mean and std.dev: (360.83, 151.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1352) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678908 sequences (100000060 bp) in 3.469 CPU sec, 3.596 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 283524, 2, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 262, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (306.21, 140.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (239, 377, 515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1067) [M::mem_pestat] mean and std.dev: (384.65, 177.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1343) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (126, 248, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (272.83, 146.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1198) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679654 sequences (100000119 bp) in 2.470 CPU sec, 2.469 real sec... [M::mem_process_seqs] Processed 679116 reads in 208.143 CPU sec, 6.580 real sec [M::mem_process_seqs] Processed 678722 reads in 137.962 CPU sec, 4.353 real sec sorting block 107, #reads = 1072846 mem = 536879608 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 283398, 9, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 213, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (235.09, 112.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (239, 377, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1073) [M::mem_pestat] mean and std.dev: (385.53, 178.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1351) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 296, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (289.56, 106.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678986 sequences (100000295 bp) in 3.159 CPU sec, 3.273 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 283791, 4, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 214, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000) [M::mem_pestat] mean and std.dev: (211.95, 112.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1289) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (234, 370, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062) [M::mem_pestat] mean and std.dev: (379.98, 178.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (255, 328, 752) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1746) [M::mem_pestat] mean and std.dev: (407.62, 260.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2243) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682656 sequences (100000211 bp) in 2.580 CPU sec, 2.583 real sec... [M::mem_process_seqs] Processed 678908 reads in 202.721 CPU sec, 6.405 real sec [M::mem_process_seqs] Processed 679654 reads in 134.390 CPU sec, 4.243 real sec sorting block 108, #reads = 1073799 mem = 536879556 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 283380, 1, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (266, 409, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (353.76, 129.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (237, 375, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074) [M::mem_pestat] mean and std.dev: (385.21, 181.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1353) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 355, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (337.14, 127.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686990 sequences (100000093 bp) in 3.337 CPU sec, 3.429 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 285153, 6, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 228, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (270.50, 145.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1086) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (217, 350, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054) [M::mem_pestat] mean and std.dev: (365.60, 178.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (263, 298, 539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1091) [M::mem_pestat] mean and std.dev: (349.67, 165.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1367) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683538 sequences (100000220 bp) in 2.510 CPU sec, 2.506 real sec... [M::mem_process_seqs] Processed 678986 reads in 197.694 CPU sec, 6.244 real sec sorting block 109, #reads = 1073003 mem = 536886622 peak = 1169416192 [M::mem_process_seqs] Processed 682656 reads in 133.887 CPU sec, 4.237 real sec sorting block 110, #reads = 1072990 mem = 536878016 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 286284, 6, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 265, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988) [M::mem_pestat] mean and std.dev: (301.38, 172.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1252) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 322, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999) [M::mem_pestat] mean and std.dev: (340.48, 169.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1266) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 327, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (273.53, 101.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682444 sequences (100000201 bp) in 3.069 CPU sec, 3.154 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 285308, 3, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 271, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (278.20, 119.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 342, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019) [M::mem_pestat] mean and std.dev: (355.96, 172.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1288) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 348, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972) [M::mem_pestat] mean and std.dev: (346.56, 192.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1233) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682864 sequences (100000219 bp) in 2.570 CPU sec, 2.569 real sec... [M::mem_process_seqs] Processed 686990 reads in 210.562 CPU sec, 6.662 real sec [M::mem_process_seqs] Processed 683538 reads in 136.748 CPU sec, 4.328 real sec sorting block 111, #reads = 1072620 mem = 536879040 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 284945, 2, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (262, 415, 3912) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11212) [M::mem_pestat] mean and std.dev: (1387.74, 1752.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14862) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (218, 352, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043) [M::mem_pestat] mean and std.dev: (364.40, 175.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1318) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (264, 391, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (354.39, 147.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1000) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681618 sequences (100000108 bp) in 3.352 CPU sec, 3.431 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 284695, 5, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 268, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1114) [M::mem_pestat] mean and std.dev: (277.26, 143.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (217, 350, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033) [M::mem_pestat] mean and std.dev: (361.71, 173.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1305) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 321, 632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1546) [M::mem_pestat] mean and std.dev: (355.42, 203.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2003) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680730 sequences (100000055 bp) in 2.450 CPU sec, 2.451 real sec... [M::mem_process_seqs] Processed 682444 reads in 199.348 CPU sec, 6.301 real sec [M::mem_process_seqs] Processed 682864 reads in 133.090 CPU sec, 4.201 real sec sorting block 112, #reads = 1074181 mem = 536880963 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 284474, 1, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (279, 448, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (358.62, 135.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (223, 359, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042) [M::mem_pestat] mean and std.dev: (368.08, 174.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1315) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 378, 646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1506) [M::mem_pestat] mean and std.dev: (339.15, 183.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1936) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680488 sequences (100000224 bp) in 3.159 CPU sec, 3.252 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 284215, 3, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (227, 419, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088) [M::mem_pestat] mean and std.dev: (362.50, 160.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (228, 365, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047) [M::mem_pestat] mean and std.dev: (372.82, 174.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1320) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 307, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (303.47, 137.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679446 sequences (100000166 bp) in 2.310 CPU sec, 2.308 real sec... [M::mem_process_seqs] Processed 681618 reads in 194.569 CPU sec, 6.153 real sec sorting block 113, #reads = 1073466 mem = 536881834 peak = 1169416192 [M::mem_process_seqs] Processed 680730 reads in 127.516 CPU sec, 4.023 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 284131, 6, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 317, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056) [M::mem_pestat] mean and std.dev: (288.16, 153.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (230, 367, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1049) [M::mem_pestat] mean and std.dev: (374.79, 175.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1322) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 322, 603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1375) [M::mem_pestat] mean and std.dev: (327.67, 144.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1761) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680256 sequences (100000268 bp) in 3.356 CPU sec, 3.433 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282999, 5, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 175, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (181.30, 73.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (236, 373, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055) [M::mem_pestat] mean and std.dev: (380.49, 176.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1328) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 321, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942) [M::mem_pestat] mean and std.dev: (377.77, 193.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1177) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680488 reads in 189.475 CPU sec, 5.996 real sec [M::process] read 680982 sequences (100000290 bp) in 2.150 CPU sec, 2.147 real sec... sorting block 114, #reads = 1073943 mem = 536876956 peak = 1169416192 [M::mem_process_seqs] Processed 679446 reads in 123.533 CPU sec, 3.897 real sec sorting block 115, #reads = 1073839 mem = 536878918 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283599, 1, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (328, 432, 1072) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2560) [M::mem_pestat] mean and std.dev: (526.68, 326.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3304) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (230, 367, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052) [M::mem_pestat] mean and std.dev: (376.28, 177.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1326) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (238, 350, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (314.66, 98.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680644 sequences (100000041 bp) in 3.178 CPU sec, 3.262 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 284079, 3, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 295, 677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1611) [M::mem_pestat] mean and std.dev: (412.27, 331.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2078) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (226, 362, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051) [M::mem_pestat] mean and std.dev: (372.57, 177.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1326) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 321, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (290.39, 117.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680256 reads in 186.292 CPU sec, 5.908 real sec [M::mem_process_seqs] Processed 680982 reads in 121.084 CPU sec, 3.805 real sec [M::process] read 680476 sequences (100000022 bp) in 2.656 CPU sec, 2.684 real sec... sorting block 116, #reads = 1076246 mem = 536881212 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 284024, 1, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 299, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1247) [M::mem_pestat] mean and std.dev: (294.55, 140.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (227, 364, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055) [M::mem_pestat] mean and std.dev: (373.93, 177.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1331) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 215, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (274.43, 173.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680328 sequences (100000031 bp) in 3.465 CPU sec, 3.577 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 283478, 0, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 373, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1145) [M::mem_pestat] mean and std.dev: (331.78, 168.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (228, 363, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050) [M::mem_pestat] mean and std.dev: (373.69, 176.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1324) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (288, 342, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (354.06, 143.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680644 reads in 191.599 CPU sec, 6.081 real sec [M::mem_process_seqs] Processed 680476 reads in 128.094 CPU sec, 4.030 real sec [M::process] read 680008 sequences (100000240 bp) in 2.781 CPU sec, 2.805 real sec... sorting block 117, #reads = 1076654 mem = 536877948 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 283803, 2, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 159, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051) [M::mem_pestat] mean and std.dev: (252.09, 160.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1349) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (230, 366, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052) [M::mem_pestat] mean and std.dev: (376.18, 178.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1326) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 324, 782) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1942) [M::mem_pestat] mean and std.dev: (552.94, 479.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2522) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680696 sequences (100000191 bp) in 3.167 CPU sec, 3.321 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 283160, 0, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 232, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (283.60, 171.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 999) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (231, 368, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1065) [M::mem_pestat] mean and std.dev: (378.31, 178.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1343) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 209, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (264.35, 135.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680328 reads in 188.072 CPU sec, 5.977 real sec [M::mem_process_seqs] Processed 680008 reads in 127.745 CPU sec, 4.020 real sec sorting block 118, #reads = 1076537 mem = 536878366 peak = 1169416192 [M::process] read 680158 sequences (100000093 bp) in 2.468 CPU sec, 2.508 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 283780, 5, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 291, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (281.74, 113.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (227, 362, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1049) [M::mem_pestat] mean and std.dev: (372.54, 175.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1323) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 288, 585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1361) [M::mem_pestat] mean and std.dev: (341.26, 237.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1749) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 119, #reads = 1076465 mem = 536885681 peak = 1169416192 [M::process] read 679632 sequences (100000222 bp) in 3.199 CPU sec, 3.333 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 284240, 0, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 255, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (306.02, 120.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (231, 367, 506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056) [M::mem_pestat] mean and std.dev: (376.76, 176.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1331) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 290, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1089) [M::mem_pestat] mean and std.dev: (290.59, 141.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1387) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680696 reads in 197.172 CPU sec, 6.259 real sec [M::mem_process_seqs] Processed 680158 reads in 129.707 CPU sec, 4.076 real sec [M::process] read 683198 sequences (100000162 bp) in 2.748 CPU sec, 2.759 real sec... sorting block 120, #reads = 1073899 mem = 536877408 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 283248, 0, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (257, 311, 606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1304) [M::mem_pestat] mean and std.dev: (327.97, 172.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (234, 372, 512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068) [M::mem_pestat] mean and std.dev: (381.31, 178.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1346) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 237, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (316.28, 164.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678334 sequences (100000001 bp) in 2.994 CPU sec, 3.113 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 284872, 4, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 296, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075) [M::mem_pestat] mean and std.dev: (358.09, 204.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1362) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 344, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019) [M::mem_pestat] mean and std.dev: (357.96, 172.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1287) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 254, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (234.91, 78.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677916 sequences (100000100 bp) in 2.300 CPU sec, 2.301 real sec... [M::mem_process_seqs] Processed 679632 reads in 211.924 CPU sec, 6.720 real sec [M::mem_process_seqs] Processed 683198 reads in 147.154 CPU sec, 4.662 real sec sorting block 121, #reads = 1072959 mem = 536879768 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 282608, 3, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 300, 611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1475) [M::mem_pestat] mean and std.dev: (376.60, 263.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1907) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 384, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1095) [M::mem_pestat] mean and std.dev: (393.39, 184.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (277, 419, 646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1384) [M::mem_pestat] mean and std.dev: (417.62, 194.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1753) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678182 sequences (100000101 bp) in 3.372 CPU sec, 3.519 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 282285, 1, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 285, 664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1680) [M::mem_pestat] mean and std.dev: (414.05, 362.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2188) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 387, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1103) [M::mem_pestat] mean and std.dev: (396.64, 185.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1389) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 381, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1116) [M::mem_pestat] mean and std.dev: (382.55, 176.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1407) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677768 sequences (100000255 bp) in 2.409 CPU sec, 2.407 real sec... [M::mem_process_seqs] Processed 678334 reads in 195.749 CPU sec, 6.174 real sec [M::mem_process_seqs] Processed 677916 reads in 128.336 CPU sec, 4.054 real sec sorting block 122, #reads = 1071883 mem = 536877926 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 282774, 1, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 245, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1174) [M::mem_pestat] mean and std.dev: (314.09, 221.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 385, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1104) [M::mem_pestat] mean and std.dev: (395.00, 185.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1391) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 324, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943) [M::mem_pestat] mean and std.dev: (328.44, 142.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1177) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677326 sequences (100000075 bp) in 3.143 CPU sec, 3.265 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282126, 11, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 428, 642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1604) [M::mem_pestat] mean and std.dev: (360.66, 266.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2085) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 390, 535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1113) [M::mem_pestat] mean and std.dev: (398.75, 186.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1402) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1684, 1684, 1684) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1684, 1684) [M::mem_pestat] mean and std.dev: (1684.00, 0.00) [M::mem_pestat] low and high boundaries for proper pairs: (1684, 1684) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 365, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1095) [M::mem_pestat] mean and std.dev: (353.66, 160.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1384) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 678182 reads in 196.944 CPU sec, 6.241 real sec [M::process] read 677272 sequences (100000076 bp) in 2.507 CPU sec, 2.510 real sec... sorting block 123, #reads = 1075909 mem = 536878267 peak = 1169416192 [M::mem_process_seqs] Processed 677768 reads in 129.738 CPU sec, 4.088 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 282284, 2, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 277, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1072) [M::mem_pestat] mean and std.dev: (280.42, 127.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1356) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 395, 542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1128) [M::mem_pestat] mean and std.dev: (404.85, 190.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1421) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 294, 642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1514) [M::mem_pestat] mean and std.dev: (293.27, 148.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1950) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 124, #reads = 1075842 mem = 536879334 peak = 1169416192 [M::process] read 677176 sequences (100000050 bp) in 3.501 CPU sec, 3.648 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 282360, 4, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 318, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076) [M::mem_pestat] mean and std.dev: (267.75, 111.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (251, 399, 547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1139) [M::mem_pestat] mean and std.dev: (408.49, 192.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1435) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 336, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (316.58, 148.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677326 reads in 190.235 CPU sec, 6.021 real sec [M::process] read 677056 sequences (100000145 bp) in 2.593 CPU sec, 2.638 real sec... [M::mem_process_seqs] Processed 677272 reads in 124.384 CPU sec, 3.910 real sec sorting block 125, #reads = 1076139 mem = 536880226 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 282229, 7, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 276, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (232.50, 84.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (252, 400, 549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1143) [M::mem_pestat] mean and std.dev: (409.84, 193.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1440) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 308, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009) [M::mem_pestat] mean and std.dev: (318.63, 169.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1270) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677086 sequences (100000008 bp) in 3.132 CPU sec, 3.267 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 282785, 0, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 324, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (330.57, 157.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (253, 402, 551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1147) [M::mem_pestat] mean and std.dev: (411.74, 194.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1445) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 332, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997) [M::mem_pestat] mean and std.dev: (330.58, 149.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1259) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677176 reads in 189.647 CPU sec, 6.026 real sec [M::mem_process_seqs] Processed 677056 reads in 128.418 CPU sec, 4.043 real sec [M::process] read 676992 sequences (100000000 bp) in 2.874 CPU sec, 2.908 real sec... sorting block 126, #reads = 1075503 mem = 536878012 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282544, 5, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 240, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (250.57, 118.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (253, 400, 550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1144) [M::mem_pestat] mean and std.dev: (411.51, 195.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1441) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (244, 400, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042) [M::mem_pestat] mean and std.dev: (376.06, 151.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1308) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680360 sequences (100000076 bp) in 3.384 CPU sec, 3.542 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 282679, 3, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 518, 889) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2283) [M::mem_pestat] mean and std.dev: (499.69, 350.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2980) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (251, 399, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1157) [M::mem_pestat] mean and std.dev: (412.71, 198.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1459) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 272, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (282.14, 128.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677086 reads in 193.946 CPU sec, 6.170 real sec [M::mem_process_seqs] Processed 676992 reads in 133.832 CPU sec, 4.222 real sec [M::process] read 680544 sequences (100000147 bp) in 2.746 CPU sec, 2.777 real sec... sorting block 127, #reads = 1076246 mem = 536879398 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 283587, 3, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 301, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (282.48, 128.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (230, 371, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1106) [M::mem_pestat] mean and std.dev: (386.54, 190.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1398) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 341, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (330.18, 137.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679626 sequences (100000077 bp) in 2.814 CPU sec, 2.940 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 283626, 5, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 297, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (270.97, 122.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (228, 373, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1119) [M::mem_pestat] mean and std.dev: (387.55, 191.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1416) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 244, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (288.00, 184.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679034 sequences (100000153 bp) in 2.400 CPU sec, 2.395 real sec... [M::mem_process_seqs] Processed 680360 reads in 212.285 CPU sec, 6.745 real sec sorting block 128, #reads = 1072977 mem = 536878124 peak = 1169416192 [M::mem_process_seqs] Processed 680544 reads in 130.192 CPU sec, 4.128 real sec sorting block 129, #reads = 1072460 mem = 536879658 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 282870, 1, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 247, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019) [M::mem_pestat] mean and std.dev: (292.12, 177.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1305) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (236, 383, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1130) [M::mem_pestat] mean and std.dev: (395.62, 192.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1428) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 324, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837) [M::mem_pestat] mean and std.dev: (337.16, 178.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679266 sequences (100000230 bp) in 3.565 CPU sec, 3.736 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282737, 6, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 212, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (213.21, 85.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (240, 387, 536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1128) [M::mem_pestat] mean and std.dev: (398.69, 193.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1424) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 235, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (249.13, 90.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 679626 reads in 185.077 CPU sec, 5.900 real sec [M::mem_process_seqs] Processed 679034 reads in 121.210 CPU sec, 3.858 real sec [M::process] read 679034 sequences (100000169 bp) in 2.853 CPU sec, 2.921 real sec... sorting block 130, #reads = 1080467 mem = 536882686 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283242, 4, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 432, 616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1502) [M::mem_pestat] mean and std.dev: (368.18, 228.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1945) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (239, 384, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1118) [M::mem_pestat] mean and std.dev: (395.82, 191.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1411) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (325, 421, 747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1591) [M::mem_pestat] mean and std.dev: (441.11, 251.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2013) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678384 sequences (100000248 bp) in 3.022 CPU sec, 3.125 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 283076, 3, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 246, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (220.18, 99.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (239, 383, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1109) [M::mem_pestat] mean and std.dev: (394.55, 189.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1399) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 350, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (318.81, 95.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 679266 reads in 177.638 CPU sec, 5.653 real sec [M::mem_process_seqs] Processed 679034 reads in 116.597 CPU sec, 3.668 real sec [M::process] read 677680 sequences (100000148 bp) in 2.973 CPU sec, 3.049 real sec... sorting block 131, #reads = 1080040 mem = 536878696 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 282426, 4, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (297, 297, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (265, 345) [M::mem_pestat] mean and std.dev: (298.86, 4.29) [M::mem_pestat] low and high boundaries for proper pairs: (249, 361) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 390, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1107) [M::mem_pestat] mean and std.dev: (398.86, 188.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1394) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 308, 478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054) [M::mem_pestat] mean and std.dev: (267.18, 118.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1342) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677470 sequences (100000276 bp) in 3.332 CPU sec, 3.502 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282933, 2, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 231, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (185.11, 67.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (251, 396, 536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1106) [M::mem_pestat] mean and std.dev: (403.27, 188.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1391) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (271, 370, 586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1216) [M::mem_pestat] mean and std.dev: (388.63, 163.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1531) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 678384 reads in 169.964 CPU sec, 5.414 real sec [M::mem_process_seqs] Processed 677680 reads in 108.936 CPU sec, 3.440 real sec sorting block 132, #reads = 1078973 mem = 536879294 peak = 1169416192 [M::process] read 677258 sequences (100000144 bp) in 2.609 CPU sec, 2.712 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282534, 0, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 329, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (22, 547) [M::mem_pestat] mean and std.dev: (304.26, 99.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (251, 395, 536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1106) [M::mem_pestat] mean and std.dev: (403.44, 189.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1391) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 553, 744) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1814) [M::mem_pestat] mean and std.dev: (445.90, 243.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2349) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 133, #reads = 1079850 mem = 536880386 peak = 1169416192 [M::process] read 677286 sequences (100000169 bp) in 2.432 CPU sec, 2.586 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 282074, 2, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 341, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016) [M::mem_pestat] mean and std.dev: (333.96, 190.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1283) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (252, 398, 542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1122) [M::mem_pestat] mean and std.dev: (406.18, 189.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1412) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 287, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (252.41, 108.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677470 reads in 174.891 CPU sec, 5.560 real sec [M::mem_process_seqs] Processed 677258 reads in 115.146 CPU sec, 3.629 real sec [M::process] read 677364 sequences (100000067 bp) in 2.172 CPU sec, 2.185 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 282615, 2, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (240, 240, 544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1152) [M::mem_pestat] mean and std.dev: (317.81, 162.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (255, 402, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1125) [M::mem_pestat] mean and std.dev: (410.24, 192.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1415) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 260, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (300.04, 156.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1167) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 134, #reads = 1077604 mem = 536879599 peak = 1169416192 [M::process] read 678708 sequences (100000139 bp) in 3.112 CPU sec, 3.291 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282887, 6, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 349, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940) [M::mem_pestat] mean and std.dev: (307.14, 133.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1187) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (253, 400, 542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1120) [M::mem_pestat] mean and std.dev: (408.43, 192.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1409) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 214, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (251.64, 122.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677286 reads in 166.999 CPU sec, 5.312 real sec [M::mem_process_seqs] Processed 677364 reads in 108.083 CPU sec, 3.410 real sec [M::process] read 680206 sequences (100000189 bp) in 2.862 CPU sec, 2.944 real sec... sorting block 135, #reads = 1078600 mem = 536880506 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 283197, 2, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 272, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (270.20, 107.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (240, 384, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1107) [M::mem_pestat] mean and std.dev: (394.91, 188.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1396) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 314, 1022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2588) [M::mem_pestat] mean and std.dev: (595.42, 524.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3371) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678980 sequences (100000159 bp) in 2.635 CPU sec, 2.733 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283590, 1, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (227, 301, 908) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2270) [M::mem_pestat] mean and std.dev: (323.96, 216.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2951) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (230, 370, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1094) [M::mem_pestat] mean and std.dev: (384.37, 187.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1382) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (247, 324, 613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1345) [M::mem_pestat] mean and std.dev: (357.48, 170.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1711) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 678708 reads in 174.127 CPU sec, 5.538 real sec [M::mem_process_seqs] Processed 680206 reads in 117.130 CPU sec, 3.691 real sec [M::process] read 678646 sequences (100000272 bp) in 2.790 CPU sec, 2.832 real sec... sorting block 136, #reads = 1079292 mem = 536880166 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 283212, 4, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 192, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1110) [M::mem_pestat] mean and std.dev: (347.45, 306.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (239, 383, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1103) [M::mem_pestat] mean and std.dev: (393.54, 187.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1391) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 275, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848) [M::mem_pestat] mean and std.dev: (332.41, 186.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1077) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678396 sequences (100000199 bp) in 3.212 CPU sec, 3.355 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282915, 3, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 320, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (289.14, 119.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 388, 536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1122) [M::mem_pestat] mean and std.dev: (399.58, 191.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1415) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 398, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (355.37, 159.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 678980 reads in 179.292 CPU sec, 5.700 real sec [M::mem_process_seqs] Processed 678646 reads in 116.128 CPU sec, 3.664 real sec sorting block 137, #reads = 1079788 mem = 536876809 peak = 1169416192 [M::process] read 678234 sequences (100000156 bp) in 2.932 CPU sec, 2.988 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 283507, 0, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 393, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1071) [M::mem_pestat] mean and std.dev: (337.96, 159.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1367) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 389, 536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1120) [M::mem_pestat] mean and std.dev: (399.86, 190.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1412) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (271, 273, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 517) [M::mem_pestat] mean and std.dev: (291.32, 89.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 138, #reads = 1078987 mem = 536882835 peak = 1169416192 [M::process] read 677872 sequences (100000026 bp) in 2.735 CPU sec, 2.967 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 283223, 0, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 159, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (193.59, 90.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 390, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1112) [M::mem_pestat] mean and std.dev: (399.15, 188.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1401) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (259, 326, 610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1312) [M::mem_pestat] mean and std.dev: (376.52, 209.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1663) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 678396 reads in 186.716 CPU sec, 5.939 real sec [M::mem_process_seqs] Processed 678234 reads in 127.115 CPU sec, 4.008 real sec [M::process] read 677052 sequences (100000108 bp) in 2.734 CPU sec, 2.780 real sec... sorting block 139, #reads = 1077408 mem = 536879094 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283365, 4, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 227, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1191) [M::mem_pestat] mean and std.dev: (258.81, 188.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 396, 544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1134) [M::mem_pestat] mean and std.dev: (406.30, 193.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1429) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (268, 333, 540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1084) [M::mem_pestat] mean and std.dev: (400.70, 195.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1356) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677322 sequences (100000246 bp) in 3.291 CPU sec, 3.442 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282156, 5, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 414, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034) [M::mem_pestat] mean and std.dev: (309.33, 146.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1316) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (252, 400, 547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1137) [M::mem_pestat] mean and std.dev: (409.31, 193.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1432) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (263, 299, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (302.97, 130.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677872 reads in 184.292 CPU sec, 5.854 real sec [M::mem_process_seqs] Processed 677052 reads in 124.460 CPU sec, 3.921 real sec [M::process] read 677050 sequences (100000190 bp) in 2.882 CPU sec, 2.940 real sec... sorting block 140, #reads = 1077812 mem = 536880336 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 282080, 5, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (255, 382, 607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1311) [M::mem_pestat] mean and std.dev: (351.06, 147.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1663) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (252, 400, 547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1137) [M::mem_pestat] mean and std.dev: (410.01, 195.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1432) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 378, 535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1131) [M::mem_pestat] mean and std.dev: (337.43, 146.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1429) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678206 sequences (100000034 bp) in 3.035 CPU sec, 3.142 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 282220, 3, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 256, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1206) [M::mem_pestat] mean and std.dev: (288.11, 171.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (253, 399, 550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1144) [M::mem_pestat] mean and std.dev: (410.88, 195.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1441) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (267, 367, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1065) [M::mem_pestat] mean and std.dev: (354.68, 156.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1331) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677322 reads in 187.595 CPU sec, 5.961 real sec [M::mem_process_seqs] Processed 677050 reads in 127.485 CPU sec, 4.011 real sec [M::process] read 678700 sequences (100000225 bp) in 3.011 CPU sec, 3.069 real sec... sorting block 141, #reads = 1078646 mem = 536879201 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 282969, 6, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 328, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (278.86, 101.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1009) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 387, 535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1117) [M::mem_pestat] mean and std.dev: (399.41, 191.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1408) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 335, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (306.28, 147.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1014) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677664 sequences (100000229 bp) in 3.068 CPU sec, 3.193 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 283708, 1, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 243, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (288.04, 138.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (241, 383, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1108) [M::mem_pestat] mean and std.dev: (395.71, 190.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1397) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 501, 856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2098) [M::mem_pestat] mean and std.dev: (447.51, 283.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2719) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 678206 reads in 198.093 CPU sec, 6.287 real sec [M::process] read 677658 sequences (100000017 bp) in 2.423 CPU sec, 2.428 real sec... sorting block 142, #reads = 1077665 mem = 536880155 peak = 1169416192 [M::mem_process_seqs] Processed 678700 reads in 131.187 CPU sec, 4.137 real sec sorting block 143, #reads = 1073690 mem = 536879140 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 282582, 2, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 316, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (312.67, 141.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1072) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 395, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1141) [M::mem_pestat] mean and std.dev: (406.39, 194.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1439) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 375, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1099) [M::mem_pestat] mean and std.dev: (399.27, 279.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1519) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677450 sequences (100000110 bp) in 2.654 CPU sec, 2.799 real sec... [M::process] read 677576 sequences (100000205 bp) in 1.760 CPU sec, 1.759 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 283296, 0, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 372, 515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1125) [M::mem_pestat] mean and std.dev: (337.06, 179.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1430) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 393, 546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1144) [M::mem_pestat] mean and std.dev: (406.19, 195.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1443) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (278, 423, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (362.56, 141.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677664 reads in 192.085 CPU sec, 6.114 real sec [M::mem_process_seqs] Processed 677658 reads in 124.623 CPU sec, 3.960 real sec sorting block 144, #reads = 1075688 mem = 536878165 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 282150, 0, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 279, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953) [M::mem_pestat] mean and std.dev: (317.55, 182.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1213) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 396, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1137) [M::mem_pestat] mean and std.dev: (407.05, 193.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1433) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 396, 563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1225) [M::mem_pestat] mean and std.dev: (386.46, 166.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1556) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678086 sequences (100000294 bp) in 3.270 CPU sec, 3.385 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 282964, 5, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 178, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (197.83, 86.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 393, 548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1150) [M::mem_pestat] mean and std.dev: (407.11, 196.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1451) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 326, 565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1277) [M::mem_pestat] mean and std.dev: (330.55, 157.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1633) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677450 reads in 186.477 CPU sec, 5.886 real sec [M::process] read 677482 sequences (100000099 bp) in 2.340 CPU sec, 2.344 real sec... [M::mem_process_seqs] Processed 677576 reads in 121.264 CPU sec, 3.820 real sec sorting block 145, #reads = 1076636 mem = 536879494 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 283103, 3, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (260, 400, 615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1325) [M::mem_pestat] mean and std.dev: (394.14, 201.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 390, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1149) [M::mem_pestat] mean and std.dev: (404.21, 195.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1451) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 244, 699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1625) [M::mem_pestat] mean and std.dev: (293.57, 156.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2088) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677650 sequences (100000206 bp) in 3.201 CPU sec, 3.365 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 282892, 2, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (292, 433, 605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1231) [M::mem_pestat] mean and std.dev: (459.29, 243.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 397, 555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1169) [M::mem_pestat] mean and std.dev: (412.36, 201.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1476) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (283, 398, 594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1216) [M::mem_pestat] mean and std.dev: (342.91, 135.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1527) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 678086 reads in 186.620 CPU sec, 5.921 real sec [M::mem_process_seqs] Processed 677482 reads in 122.186 CPU sec, 3.842 real sec [M::process] read 678668 sequences (100000066 bp) in 2.695 CPU sec, 2.753 real sec... sorting block 146, #reads = 1076501 mem = 536878962 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 283098, 2, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 313, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1065) [M::mem_pestat] mean and std.dev: (312.68, 157.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1350) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 394, 549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1153) [M::mem_pestat] mean and std.dev: (408.72, 198.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1455) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (279, 301, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (340.03, 151.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678022 sequences (100000247 bp) in 3.313 CPU sec, 3.457 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283419, 3, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 230, 1690) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4738) [M::mem_pestat] mean and std.dev: (362.25, 427.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6262) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (238, 381, 535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1129) [M::mem_pestat] mean and std.dev: (398.18, 195.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1426) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 303, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (335.05, 165.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1169) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677650 reads in 199.377 CPU sec, 6.326 real sec sorting block 147, #reads = 1076082 mem = 536877222 peak = 1169416192 [M::process] read 680938 sequences (100000028 bp) in 2.637 CPU sec, 2.658 real sec... [M::mem_process_seqs] Processed 678668 reads in 134.455 CPU sec, 4.232 real sec sorting block 148, #reads = 1074415 mem = 536882694 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 282642, 0, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 237, 610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1488) [M::mem_pestat] mean and std.dev: (257.09, 158.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1927) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 386, 539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1131) [M::mem_pestat] mean and std.dev: (401.33, 194.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1427) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 407, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1080) [M::mem_pestat] mean and std.dev: (344.83, 163.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1377) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683390 sequences (100000139 bp) in 2.941 CPU sec, 3.090 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 283775, 1, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (299, 410, 563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1091) [M::mem_pestat] mean and std.dev: (404.94, 187.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (226, 364, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1111) [M::mem_pestat] mean and std.dev: (383.88, 191.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1406) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (300, 371, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969) [M::mem_pestat] mean and std.dev: (334.20, 140.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1192) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681674 sequences (100000276 bp) in 2.480 CPU sec, 2.477 real sec... [M::mem_process_seqs] Processed 678022 reads in 213.102 CPU sec, 6.764 real sec [M::mem_process_seqs] Processed 680938 reads in 141.159 CPU sec, 4.470 real sec sorting block 149, #reads = 1070110 mem = 536876760 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 285600, 3, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 326, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1078) [M::mem_pestat] mean and std.dev: (357.66, 163.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1365) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 349, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1086) [M::mem_pestat] mean and std.dev: (369.97, 187.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1377) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 250, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (279.00, 149.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680300 sequences (100000109 bp) in 3.408 CPU sec, 3.541 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 284475, 4, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 260, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (253.90, 101.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (224, 367, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1103) [M::mem_pestat] mean and std.dev: (381.06, 188.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1396) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 273, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (298.20, 149.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679970 sequences (100000286 bp) in 2.530 CPU sec, 2.532 real sec... [M::mem_process_seqs] Processed 683390 reads in 198.150 CPU sec, 6.253 real sec [M::mem_process_seqs] Processed 681674 reads in 131.357 CPU sec, 4.145 real sec sorting block 150, #reads = 1074187 mem = 536878795 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 284023, 0, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 272, 630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1566) [M::mem_pestat] mean and std.dev: (344.00, 263.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2034) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (231, 375, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1107) [M::mem_pestat] mean and std.dev: (386.82, 187.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1399) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (299, 445, 1180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2942) [M::mem_pestat] mean and std.dev: (415.07, 259.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3823) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678942 sequences (100000117 bp) in 3.073 CPU sec, 3.192 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 283694, 0, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 229, 700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1780) [M::mem_pestat] mean and std.dev: (340.48, 242.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2320) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (234, 381, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1113) [M::mem_pestat] mean and std.dev: (390.64, 188.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1406) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 313, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (273.84, 97.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680300 reads in 200.435 CPU sec, 6.351 real sec [M::process] read 679356 sequences (100000265 bp) in 2.635 CPU sec, 2.639 real sec... sorting block 151, #reads = 1075927 mem = 536877464 peak = 1169416192 [M::mem_process_seqs] Processed 679970 reads in 133.616 CPU sec, 4.217 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 284037, 0, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 465, 856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2148) [M::mem_pestat] mean and std.dev: (550.53, 465.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2794) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (241, 385, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1099) [M::mem_pestat] mean and std.dev: (393.92, 186.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1385) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (305, 388, 852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1946) [M::mem_pestat] mean and std.dev: (524.08, 364.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2493) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 152, #reads = 1075905 mem = 536879829 peak = 1169416192 [M::process] read 678294 sequences (100000177 bp) in 3.438 CPU sec, 3.559 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 283839, 3, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (269, 286, 665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1457) [M::mem_pestat] mean and std.dev: (382.24, 248.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1853) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (237, 381, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1104) [M::mem_pestat] mean and std.dev: (391.42, 187.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1393) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 296, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848) [M::mem_pestat] mean and std.dev: (283.91, 92.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 678942 reads in 195.572 CPU sec, 6.191 real sec [M::process] read 678046 sequences (100000251 bp) in 2.761 CPU sec, 2.784 real sec... [M::mem_process_seqs] Processed 679356 reads in 130.971 CPU sec, 4.115 real sec sorting block 153, #reads = 1075320 mem = 536882894 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282980, 1, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 283, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (324.34, 181.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1169) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 391, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1099) [M::mem_pestat] mean and std.dev: (397.67, 185.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1383) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 458, 601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1349) [M::mem_pestat] mean and std.dev: (395.00, 207.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1723) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677624 sequences (100000091 bp) in 3.300 CPU sec, 3.482 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 283465, 4, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 182, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (246.00, 154.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 393, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1099) [M::mem_pestat] mean and std.dev: (397.43, 183.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1383) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 346, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (277.96, 131.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1091) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 678294 reads in 194.161 CPU sec, 6.169 real sec [M::process] read 677788 sequences (100000098 bp) in 2.389 CPU sec, 2.421 real sec... [M::mem_process_seqs] Processed 678046 reads in 127.358 CPU sec, 4.008 real sec sorting block 154, #reads = 1074493 mem = 536881415 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 283115, 5, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 381, 717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1625) [M::mem_pestat] mean and std.dev: (395.14, 247.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2079) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (252, 398, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1107) [M::mem_pestat] mean and std.dev: (403.04, 185.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1392) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 262, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (253.36, 95.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678642 sequences (100000049 bp) in 3.314 CPU sec, 3.453 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282588, 7, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 284, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (313.56, 164.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (250, 397, 535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1105) [M::mem_pestat] mean and std.dev: (401.20, 185.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1390) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 287, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (287.53, 160.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1168) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677624 reads in 183.295 CPU sec, 5.813 real sec [M::mem_process_seqs] Processed 677788 reads in 120.454 CPU sec, 3.790 real sec [M::process] read 678416 sequences (100000227 bp) in 2.538 CPU sec, 2.575 real sec... sorting block 155, #reads = 1074966 mem = 536879361 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 283145, 0, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (229, 331, 658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1516) [M::mem_pestat] mean and std.dev: (331.50, 163.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1945) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (242, 384, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1100) [M::mem_pestat] mean and std.dev: (392.85, 184.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1386) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 338, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (311.39, 128.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677804 sequences (100000185 bp) in 2.919 CPU sec, 3.063 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 283035, 0, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 296, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (254.26, 92.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 992) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 383, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1083) [M::mem_pestat] mean and std.dev: (391.80, 183.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1363) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (286, 364, 849) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1975) [M::mem_pestat] mean and std.dev: (446.94, 329.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2538) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 678642 reads in 181.926 CPU sec, 5.776 real sec [M::mem_process_seqs] Processed 678416 reads in 118.191 CPU sec, 3.720 real sec sorting block 156, #reads = 1074602 mem = 536880360 peak = 1169416192 [M::process] read 677696 sequences (100000102 bp) in 2.734 CPU sec, 2.758 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 282143, 1, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 316, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (283.18, 124.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 387, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1090) [M::mem_pestat] mean and std.dev: (395.81, 183.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1371) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 279, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (285.28, 98.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 157, #reads = 1077260 mem = 536877388 peak = 1169416192 [M::process] read 677270 sequences (100000064 bp) in 3.408 CPU sec, 3.567 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282568, 1, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 344, 2754) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7908) [M::mem_pestat] mean and std.dev: (801.39, 1219.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 390, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1092) [M::mem_pestat] mean and std.dev: (397.67, 184.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1373) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 266, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (259.07, 102.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677804 reads in 187.801 CPU sec, 5.959 real sec [M::mem_process_seqs] Processed 677696 reads in 118.861 CPU sec, 3.739 real sec [M::process] read 677608 sequences (100000247 bp) in 2.270 CPU sec, 2.300 real sec... sorting block 158, #reads = 1075696 mem = 536878943 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282589, 1, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 212, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (246.29, 91.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (250, 390, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1090) [M::mem_pestat] mean and std.dev: (398.04, 182.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1370) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 339, 557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1239) [M::mem_pestat] mean and std.dev: (365.27, 244.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1580) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 678022 sequences (100000087 bp) in 3.171 CPU sec, 3.299 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282649, 9, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 315, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1126) [M::mem_pestat] mean and std.dev: (315.93, 139.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1444) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 386, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1073) [M::mem_pestat] mean and std.dev: (392.91, 179.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1348) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (303, 584, 995) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2379) [M::mem_pestat] mean and std.dev: (494.21, 330.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3071) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677270 reads in 188.402 CPU sec, 5.983 real sec [M::mem_process_seqs] Processed 677608 reads in 125.201 CPU sec, 3.934 real sec [M::process] read 677498 sequences (100000176 bp) in 2.836 CPU sec, 2.872 real sec... sorting block 159, #reads = 1076148 mem = 536879076 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 282854, 0, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 165, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (177.89, 74.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (242, 378, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055) [M::mem_pestat] mean and std.dev: (385.77, 176.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1326) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 367, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (350.62, 125.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677480 sequences (100000199 bp) in 2.766 CPU sec, 2.865 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 282259, 0, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (268, 295, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (334.91, 145.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 380, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062) [M::mem_pestat] mean and std.dev: (390.16, 179.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 275, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998) [M::mem_pestat] mean and std.dev: (317.58, 201.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1271) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677406 sequences (100000161 bp) in 1.810 CPU sec, 1.814 real sec... [M::mem_process_seqs] Processed 678022 reads in 187.143 CPU sec, 5.949 real sec [M::mem_process_seqs] Processed 677498 reads in 124.613 CPU sec, 3.941 real sec sorting block 160, #reads = 1074063 mem = 536877524 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282490, 3, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 316, 546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1212) [M::mem_pestat] mean and std.dev: (361.76, 217.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 380, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062) [M::mem_pestat] mean and std.dev: (390.69, 180.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 336, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (332.07, 124.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1181) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677716 sequences (100000026 bp) in 2.964 CPU sec, 3.123 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 281419, 3, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 299, 560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1280) [M::mem_pestat] mean and std.dev: (363.05, 220.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 380, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066) [M::mem_pestat] mean and std.dev: (391.64, 181.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1339) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 285, 588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1354) [M::mem_pestat] mean and std.dev: (340.41, 160.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1737) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677480 reads in 184.378 CPU sec, 5.845 real sec sorting block 161, #reads = 1073795 mem = 536877666 peak = 1169416192 [M::mem_process_seqs] Processed 677406 reads in 119.873 CPU sec, 3.771 real sec [M::process] read 677200 sequences (100000055 bp) in 2.725 CPU sec, 2.749 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281550, 5, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 286, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1112) [M::mem_pestat] mean and std.dev: (320.00, 170.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1404) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 380, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076) [M::mem_pestat] mean and std.dev: (391.93, 182.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1353) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 339, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (349.65, 168.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 162, #reads = 1075279 mem = 536882439 peak = 1169416192 [M::process] read 677310 sequences (100000200 bp) in 2.976 CPU sec, 3.073 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 281815, 2, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 264, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (260.28, 124.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 380, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066) [M::mem_pestat] mean and std.dev: (391.58, 181.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1339) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 286, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (281.00, 91.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677716 reads in 184.473 CPU sec, 5.850 real sec [M::process] read 677460 sequences (100000280 bp) in 2.503 CPU sec, 2.517 real sec... [M::mem_process_seqs] Processed 677200 reads in 121.256 CPU sec, 3.816 real sec sorting block 163, #reads = 1076756 mem = 536879459 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 281607, 3, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 379, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000) [M::mem_pestat] mean and std.dev: (348.50, 162.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1263) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 377, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052) [M::mem_pestat] mean and std.dev: (388.72, 179.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1321) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 342, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (279.73, 112.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 677610 sequences (100000275 bp) in 3.136 CPU sec, 3.293 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 282132, 3, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 351, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (304.39, 102.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 379, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1079) [M::mem_pestat] mean and std.dev: (393.08, 184.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1357) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 344, 606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1414) [M::mem_pestat] mean and std.dev: (347.36, 200.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1818) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677310 reads in 188.255 CPU sec, 5.960 real sec [M::process] read 677150 sequences (100000251 bp) in 2.599 CPU sec, 2.611 real sec... [M::mem_process_seqs] Processed 677460 reads in 123.329 CPU sec, 3.878 real sec sorting block 164, #reads = 1075973 mem = 536881632 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 281977, 1, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (242, 351, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (300.42, 97.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 376, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066) [M::mem_pestat] mean and std.dev: (389.78, 182.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1340) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 345, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954) [M::mem_pestat] mean and std.dev: (333.88, 137.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1193) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676948 sequences (100000125 bp) in 3.376 CPU sec, 3.520 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 282021, 3, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (256, 374, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (353.91, 159.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1104) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 382, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1082) [M::mem_pestat] mean and std.dev: (395.67, 185.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1360) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 387, 596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1454) [M::mem_pestat] mean and std.dev: (325.18, 193.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1883) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 677610 reads in 187.923 CPU sec, 5.950 real sec [M::mem_process_seqs] Processed 677150 reads in 122.740 CPU sec, 3.861 real sec [M::process] read 677002 sequences (100000108 bp) in 2.728 CPU sec, 2.767 real sec... sorting block 165, #reads = 1077917 mem = 536883705 peak = 1169416192 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 281668, 5, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 340, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044) [M::mem_pestat] mean and std.dev: (317.11, 161.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1328) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 380, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1067) [M::mem_pestat] mean and std.dev: (392.80, 181.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1340) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 309, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992) [M::mem_pestat] mean and std.dev: (285.86, 118.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1257) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 676958 sequences (100000012 bp) in 3.145 CPU sec, 3.306 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281186, 4, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 312, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (285.52, 150.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1001) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 382, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068) [M::mem_pestat] mean and std.dev: (394.36, 183.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 309, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1203) [M::mem_pestat] mean and std.dev: (321.29, 140.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1536) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676948 reads in 189.997 CPU sec, 6.037 real sec sorting block 166, #reads = 1078111 mem = 536880281 peak = 1169416192 [M::mem_process_seqs] Processed 677002 reads in 124.770 CPU sec, 3.930 real sec [M::process] read 677172 sequences (100000271 bp) in 2.861 CPU sec, 2.897 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282038, 1, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 281, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (281.64, 111.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 382, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1073) [M::mem_pestat] mean and std.dev: (394.99, 184.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1348) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 351, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016) [M::mem_pestat] mean and std.dev: (293.94, 125.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1281) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 167, #reads = 1077332 mem = 536877435 peak = 1169416192 [M::process] read 676886 sequences (100000034 bp) in 3.186 CPU sec, 3.309 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 281955, 1, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 227, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (296.22, 176.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1187) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 384, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1094) [M::mem_pestat] mean and std.dev: (398.25, 187.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1376) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 232, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (248.82, 103.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676958 reads in 185.059 CPU sec, 5.874 real sec [M::process] read 676822 sequences (100000112 bp) in 2.771 CPU sec, 2.810 real sec... [M::process] read 220036 sequences (32408992 bp) in 1.020 CPU sec, 1.020 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 281842, 0, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 208, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984) [M::mem_pestat] mean and std.dev: (261.10, 107.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1244) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (250, 388, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1096) [M::mem_pestat] mean and std.dev: (400.82, 188.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1378) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 276, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (309.47, 159.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1030) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676886 reads in 102.353 CPU sec, 3.264 real sec sorting block 168, #reads = 1078600 mem = 536880153 peak = 1169416192 [M::mem_process_seqs] Processed 677172 reads in 254.754 CPU sec, 8.091 real sec sorting block 169, #reads = 1078339 mem = 536882513 peak = 1231437824 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 281900, 0, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 341, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (304.86, 147.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1096) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (250, 388, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1099) [M::mem_pestat] mean and std.dev: (400.96, 188.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1382) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 316, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003) [M::mem_pestat] mean and std.dev: (321.28, 125.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1271) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 91754, 1, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 190, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (232.50, 166.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (238, 371, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1063) [M::mem_pestat] mean and std.dev: (385.77, 183.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 231, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (84, 449) [M::mem_pestat] mean and std.dev: (244.47, 59.56) [M::mem_pestat] low and high boundaries for proper pairs: (6, 522) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 676822 reads in 151.645 CPU sec, 4.907 real sec [M::mem_process_seqs] Processed 220036 reads in 55.410 CPU sec, 1.796 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000606a6 features: 1f8bfbff fffa320b extended: f1bf07ab 00407f5a amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8375C CPU @ 2.90GHz threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 183249522 overall: 69923004416 mem 31090.034 user 801.617 sys 1093.868 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_3LU-T1-TDNA-1_A23MHWWLT4_1.L001\tPU:L001\tSM:1136_3LU-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_3LU-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1136_3LU-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 1136_3LU-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 1093.903 sec; CPU: 31891.656 sec sorting block 170, #reads = 1016083 mem = 507066674 peak = 1231437824 uniq header count 1 merging 171 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000606a6 features: 1f8bfbff fffa320b extended: f1bf07ab 00407f5a amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8375C CPU @ 2.90GHz threads: 31 max 128 algo: util-sort license: sentieon:util=1 output file size: 10281516743 output reads: 184244651 bam_mem_sort: 171 calls 178.497 user 3.446 sys 182.766 real bam_write: 171 calls 83.716 user 6.277 sys 119.253 real execute: 1 calls 58.868 user 1.769 sys 1175.865 real merge_files: 1 calls 58.818 user 1.760 sys 80.631 real parse_chunk: 2736 calls 173.497 user 39.038 sys 222.581 real read_chunk: 7372 calls 89.566 user 54.180 sys 1039.708 real sort_block: 1 calls 61.673 user 52.423 sys 1002.162 real write_chunk: 4634 calls 58.313 user 1.368 sys 79.335 real overall: 3325202432 mem 3269.959 user 133.419 sys 1175.889 real ls: cannot access '*.cram': No such file or directory